Rw4G018870

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000080
Physical Location & Seq
Forward (+)
41739798 .. 41741177
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018870.1

Sequence Viewer

Length: 1380 bp
ATGACTGCAGTTGAGGGAAGAGAAAGTGCATTATTTTTTATCAATGGTCCTGGAGGTACTGGGAAGACGTACTTATATCGTGCATTGCTAGCAAAGTTGAGAAGAATGAATCATATAGTCTTAGCCACAACATCATCTGGAATTTCTGCAAATTTATTGTCCGGCGGGAGGACAGCTCACTCTAGATTCAAGATTCCACTCAATATAGATGCATCATCCATGTGTTCCATAAGTAAACAGTCTGCTCTAGCACGACTGATACAAGATTCCACTGCAATTATTTGGGATGAGACACCTATGACACATCGACATGTGTTTGAAGCACTTGATCGAACATTTCGAGACATAATGGATGTCGAATTACCGTTTGGTGGAAAGATAATGATTTTTGGGGGAGATTTTCGTCAAGTTCTCCCTGTTATTTCTAAAGGAACGAAGTCTGAACTCATTCAAGCCTCCATTGTGAAAGCAACATTTTGGGCACAAACAAAGATATTGAGATTGAGACAGAATATGAGATCCATAAATGACCACCAATTTGCAGAATTTCTACTTCGTGTTGGCGATGGGAGTAAACAAGTCATTGACGACGAAATGATGAGATTACCAGAATGCATGGTGGTTCCATGGGAAAGTGACCAATCTATCAATCAAATTATTGATGAAGTTTTCCCCAATTTGAGTGATCATGTAAATGATGTAAGATACATGGTGGACAGAGCATTGATTACTCCTATAAACGATGATGTAGACGTGTTGAATGAAAAGATAATCAATATGTTTCCAGGTGAAGAAATTACATTGTACTCATTTGACTCTGTTGAAGATGACATGAGAAATTCGTACCAACCAGAGTTTCTGAACTCAATAACCGCAGGTGGGCTGCAACCCCACAAGTTAACTTTGAAAGTAGGTGCTCCAATCATGCTTTTAAGGAATATTGACCCTATATTGGGATTGTGTAACGATACTAGATTGTTGTGTCGTGGTTCGTACCAAAATCTGATTGATGCTGAGATACTAACAGGACAGTTTGCTGGGACTAGAGTTTTTCTACCTAGAATTCCTTTGAAAAGTGCAGAAAATGCCGGGCTACCATTTCAACTAACAAGAAAGCAATTTCCAGTCAAACTGAGCTTCTCTTTGACCATAAACAAATCTCAAGGTCAGACTATCCCACATGTAGGTGTTTACCTCCTAGATCATGTTTTTAGTCATGGACAATTATATGTTGCATTGTCGAGAGGAGTATCAGAGTCAACCACAAAAGTTCTGGTTAAAAAAGGATCCATAGTTGGTGAAGATGGCGTATTTACGAAGAATGTCGTATTTAAAGAAGTTTTCCTTCATTCAAACACAAATGATCGATCGTCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

459

Amino Acids

51.22

Weight (kDa)

7.18

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 3 - 193 1.1e-68 PIF1-like helicase
Pif1_2B_dom PF21530 285 - 327 4.2e-17 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 866
Acc36I ACCTGC 1 cut(s) 866
AccI GTMKAC 1 cut(s) 750
AciI CCGC 2 cut(s) 165, 873
AclWI GGATC 3 cut(s) 513, 1281, 1294
AcsI RAATTY 5 cut(s) 141, 151, 545, 838, 1062
AfaI GTAC 5 cut(s) 58, 71, 806, 845, 995
AfiI CCNNNNNNNGG 6 cut(s) 168, 371, 879, 952, 953, 1184
AflIII ACRYGT 3 cut(s) 310, 753, 1180
AgsI TTSAA 9 cut(s) 190, 320, 452, 760, 824, 907, 1072, 1103, 1353
AjiI CACGTC 1 cut(s) 754
AjnI CCWGG 2 cut(s) 49, 784
AjuI GAANNNNNNNTTGG 4 cut(s) 351, 383, 840, 872
AluBI AGCT 2 cut(s) 176, 1137
AluI AGCT 2 cut(s) 176, 1137
Alw21I GWGCWC 1 cut(s) 919
Alw26I GTCTC 3 cut(s) 284, 336, 499
AlwI GGATC 3 cut(s) 513, 1281, 1294
ApeKI GCWGC 1 cut(s) 883
ApoI RAATTY 5 cut(s) 141, 151, 545, 838, 1062
ArsI GACNNNNNNTTYG 4 cut(s) 143, 175, 521, 553
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 47
AsuC2I CCSGG 1 cut(s) 1090
AsuHPI GGTGA 2 cut(s) 800, 1310
AsuNHI GCTAGC 1 cut(s) 88
AvaII GGWCC 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 484
BamHI GGATCC 1 cut(s) 1286
BbsI GAAGAC 2 cut(s) 71, 1365
Bbv12I GWGCWC 1 cut(s) 919
BbvI GCAGC 1 cut(s) 870
BccI CCATC 2 cut(s) 560, 1298
BciT130I CCWGG 2 cut(s) 51, 786
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 1090
BcoDI GTCTC 3 cut(s) 284, 336, 499
BfaI CTAG 7 cut(s) 89, 183, 248, 972, 1044, 1059, 1199
BfmI CTRYAG 1 cut(s) 6
BfuAI ACCTGC 1 cut(s) 866
BisI GCNGC 1 cut(s) 884
BlsI GCNGC 1 cut(s) 885
Bme1390I CCNGG 3 cut(s) 51, 786, 1090
Bme18I GGWCC 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 754
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 2 cut(s) 624, 1288
BmrFI CCNGG 3 cut(s) 51, 786, 1090
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 3 cut(s) 199, 221, 1000
BmtI GCTAGC 1 cut(s) 92
BmuI ACTGGG 1 cut(s) 69
BpiI GAAGAC 2 cut(s) 71, 1365
BplI GAGNNNNNCTC 2 cut(s) 160, 192
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 1146
BpuMI CCSGG 1 cut(s) 1090
Bsa29I ATCGAT 1 cut(s) 1366
BsaJI CCNNGG 1 cut(s) 626
Bsc4I CCNNNNNNNGG 6 cut(s) 168, 371, 879, 952, 953, 1184
Bse1I ACTGG 2 cut(s) 64, 1124
Bse3DI GCAATG 1 cut(s) 83
BseBI CCWGG 2 cut(s) 51, 786
BseCI ATCGAT 1 cut(s) 1366
BseDI CCNNGG 1 cut(s) 626
BseGI GGATG 3 cut(s) 215, 292, 358
BseLI CCNNNNNNNGG 6 cut(s) 168, 371, 879, 952, 953, 1184
BseMI GCAATG 1 cut(s) 83
BseMII CTCAG 2 cut(s) 1005, 1124
BseNI ACTGG 2 cut(s) 64, 1124
BseRI GAGGAG 1 cut(s) 1260
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 1 cut(s) 870
BseYI CCCAGC 1 cut(s) 1037
BsgI GTGCAG 1 cut(s) 1098
Bsh1285I CGRYCG 1 cut(s) 1370
BshVI ATCGAT 1 cut(s) 1366
BsiEI CGRYCG 1 cut(s) 1370
BsiHKAI GWGCWC 1 cut(s) 919
BsiSI CCGG 2 cut(s) 162, 1089
BslFI GGGAC 1 cut(s) 1054
BslI CCNNNNNNNGG 6 cut(s) 168, 371, 879, 952, 953, 1184
BsmAI GTCTC 3 cut(s) 284, 336, 499
BsmFI GGGAC 1 cut(s) 1054
BsmI GAATGC 1 cut(s) 617
Bsp1286I GDGCHC 2 cut(s) 484, 919
Bsp143I GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
Bsp19I CCATGG 1 cut(s) 626
BspACI CCGC 2 cut(s) 165, 873
BspCNI CTCAG 2 cut(s) 1006, 1125
BspDI ATCGAT 1 cut(s) 1366
BspLI GGNNCC 2 cut(s) 624, 1288
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 866
BspOI GCTAGC 1 cut(s) 92
BspPI GGATC 3 cut(s) 513, 1281, 1294
BsrDI GCAATG 1 cut(s) 83
BsrI ACTGG 2 cut(s) 64, 1124
BssECI CCNNGG 1 cut(s) 626
BssMI GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
BssT1I CCWWGG 1 cut(s) 626
Bst2UI CCWGG 2 cut(s) 51, 786
Bst4CI ACNGT 3 cut(s) 240, 366, 1032
Bst6I CTCTTC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 1085
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 3 cut(s) 121, 1014, 1133
BstDSI CCRYGG 1 cut(s) 626
BstF5I GGATG 3 cut(s) 215, 292, 358
BstKTI GATC 7 cut(s) 331, 521, 688, 1204, 1289, 1366, 1370
BstMAI GTCTC 3 cut(s) 284, 336, 499
BstMBI GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
BstMCI CGRYCG 1 cut(s) 1370
BstMWI GCNNNNNNNGC 2 cut(s) 89, 1085
BstNI CCWGG 2 cut(s) 51, 786
BstNSI RCATGY 2 cut(s) 314, 1184
BstSCI CCNGG 3 cut(s) 49, 784, 1088
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 1 cut(s) 484
BstV1I GCAGC 1 cut(s) 870
BstV2I GAAGAC 2 cut(s) 71, 1365
BstX2I RGATCY 2 cut(s) 518, 1286
BstYI RGATCY 2 cut(s) 518, 1286
Bsu15I ATCGAT 1 cut(s) 1366
BsuTUI ATCGAT 1 cut(s) 1366
BtgI CCRYGG 1 cut(s) 626
BtgZI GCGATG 1 cut(s) 579
BtrI CACGTC 1 cut(s) 754
BtsCI GGATG 3 cut(s) 215, 292, 358
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 270
BveI ACCTGC 1 cut(s) 866
Cac8I GCNNGC 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 47
ClaI ATCGAT 1 cut(s) 1366
Csp6I GTAC 5 cut(s) 57, 70, 805, 844, 994
CviJI RGCY 6 cut(s) 125, 176, 455, 883, 1093, 1137
CviKI_1 RGCY 6 cut(s) 125, 176, 455, 883, 1093, 1137
CviQI GTAC 5 cut(s) 57, 70, 805, 844, 994
DdeI CTNAG 3 cut(s) 121, 1014, 1133
DpnI GATC 7 cut(s) 330, 520, 687, 1203, 1288, 1365, 1369
DpnII GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
DraI TTTAAA 1 cut(s) 1333
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 626
Eco47I GGWCC 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 1062
EcoRII CCWGG 2 cut(s) 49, 784
EcoT14I CCWWGG 1 cut(s) 626
EcoT22I ATGCAT 2 cut(s) 214, 617
ErhI CCWWGG 1 cut(s) 626
FalI AAGNNNNNCTT 4 cut(s) 56, 88, 1329, 1361
FaqI GGGAC 1 cut(s) 1054
FauI CCCGC 1 cut(s) 158
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 1 cut(s) 884
FokI GGATG 3 cut(s) 202, 299, 365
Fsp4HI GCNGC 1 cut(s) 884
FspBI CTAG 7 cut(s) 89, 183, 248, 972, 1044, 1059, 1199
GluI GCNGC 1 cut(s) 884
GsaI CCCAGC 1 cut(s) 1041
GsuI CTGGAG 1 cut(s) 72
HapII CCGG 2 cut(s) 162, 1089
HincII GTYRAC 2 cut(s) 900, 1260
HindII GTYRAC 2 cut(s) 900, 1260
HinfI GANTC 6 cut(s) 109, 186, 193, 266, 815, 1256
HpaI GTTAAC 1 cut(s) 900
HpaII CCGG 2 cut(s) 162, 1089
HphI GGTGA 2 cut(s) 800, 1310
Hpy166II GTNNAC 7 cut(s) 236, 575, 715, 751, 900, 1192, 1260
Hpy188I TCNGA 5 cut(s) 442, 861, 1005, 1170, 1255
Hpy188III TCNNGA 5 cut(s) 138, 183, 190, 341, 1242
Hpy8I GTNNAC 7 cut(s) 236, 575, 715, 751, 900, 1192, 1260
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 1 cut(s) 1355
HpyCH4III ACNGT 3 cut(s) 240, 366, 1032
HpyCH4IV ACGT 2 cut(s) 68, 753
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 1085
HpyF3I CTNAG 3 cut(s) 121, 1014, 1133
HpySE526I ACGT 2 cut(s) 68, 753
Ksp22I TGATCA 1 cut(s) 685
KspAI GTTAAC 1 cut(s) 900
Kzo9I GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
LmnI GCTCC 1 cut(s) 922
Lsp1109I GCAGC 1 cut(s) 870
LweI GCATC 3 cut(s) 199, 221, 1000
MaeI CTAG 7 cut(s) 89, 183, 248, 972, 1044, 1059, 1199
MaeII ACGT 2 cut(s) 68, 753
MaeIII GTNAC 2 cut(s) 635, 962
MalI GATC 7 cut(s) 330, 520, 687, 1203, 1288, 1365, 1369
MboI GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
MboII GAAGA 8 cut(s) 30, 76, 114, 803, 836, 1313, 1330, 1365
MflI RGATCY 2 cut(s) 518, 1286
MhlI GDGCHC 2 cut(s) 484, 919
MlyI GAGTC 2 cut(s) 809, 1265
MnlI CCTC 6 cut(s) 7, 47, 162, 466, 1205, 1238
Mph1103I ATGCAT 2 cut(s) 214, 617
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 4 cut(s) 899, 932, 1278, 1332
MslI CAYNNNNRTG 4 cut(s) 220, 309, 693, 1185
MspI CCGG 2 cut(s) 162, 1089
MspR9I CCNGG 3 cut(s) 51, 786, 1090
Mva1269I GAATGC 1 cut(s) 617
MvaI CCWGG 2 cut(s) 51, 786
MwoI GCNNNNNNNGC 2 cut(s) 89, 1085
NciI CCSGG 1 cut(s) 1090
NcoI CCATGG 1 cut(s) 626
NdeII GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
NheI GCTAGC 1 cut(s) 88
NlaIV GGNNCC 2 cut(s) 624, 1288
NmuCI GTSAC 1 cut(s) 635
NsiI ATGCAT 2 cut(s) 214, 617
NspI RCATGY 2 cut(s) 314, 1184
PaqCI CACCTGC 1 cut(s) 866
PciI ACATGT 2 cut(s) 310, 1180
PcsI WCGNNNNNNNCGW 1 cut(s) 337
PctI GAATGC 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 4 cut(s) 109, 186, 193, 266
PfoI TCCNGGA 1 cut(s) 49
PkrI GCNGC 1 cut(s) 885
Ple19I CGATCG 1 cut(s) 1370
PleI GAGTC 2 cut(s) 809, 1264
PpsI GAGTC 2 cut(s) 809, 1264
PscI ACATGT 2 cut(s) 310, 1180
Psp6I CCWGG 2 cut(s) 49, 784
PspFI CCCAGC 1 cut(s) 1037
PspGI CCWGG 2 cut(s) 49, 784
PspN4I GGNNCC 2 cut(s) 624, 1288
PspPI GGNCC 1 cut(s) 47
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 518, 1286
PvuI CGATCG 1 cut(s) 1370
RsaI GTAC 5 cut(s) 58, 71, 806, 845, 995
RsaNI GTAC 5 cut(s) 57, 70, 805, 844, 994
RseI CAYNNNNRTG 4 cut(s) 220, 309, 693, 1185
SaqAI TTAA 4 cut(s) 899, 932, 1278, 1332
SatI GCNGC 1 cut(s) 884
Sau3AI GATC 7 cut(s) 328, 518, 685, 1201, 1286, 1363, 1367
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 2 cut(s) 809, 1265
ScrFI CCNGG 3 cut(s) 51, 786, 1090
SduI GDGCHC 2 cut(s) 484, 919
SfaNI GCATC 3 cut(s) 199, 221, 1000
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 4 cut(s) 220, 309, 693, 1185
SmlI CTYRAG 1 cut(s) 1161
SmoI CTYRAG 1 cut(s) 1161
SsiI CCGC 2 cut(s) 165, 873
SspI AATATT 1 cut(s) 940
SspMI CTAG 7 cut(s) 89, 183, 248, 972, 1044, 1059, 1199
StyD4I CCNGG 3 cut(s) 49, 784, 1088
StyI CCWWGG 1 cut(s) 626
TaaI ACNGT 3 cut(s) 240, 366, 1032
TaiI ACGT 2 cut(s) 71, 756
TaqI TCGA 6 cut(s) 307, 331, 340, 357, 1241, 1366
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 4 cut(s) 109, 186, 193, 266
Tru1I TTAA 4 cut(s) 899, 932, 1278, 1332
Tru9I TTAA 4 cut(s) 899, 932, 1278, 1332
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 1 cut(s) 883
Tsp45I GTSAC 1 cut(s) 635
TspDTI ATGAA 5 cut(s) 122, 678, 777, 1337, 1365
TspRI CASTG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 47
XapI RAATTY 5 cut(s) 141, 151, 545, 838, 1062
XbaI TCTAGA 1 cut(s) 182
XceI RCATGY 2 cut(s) 314, 1184
XcmI CCANNNNNNNNNTGG 1 cut(s) 1270
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 7 cut(s) 89, 183, 248, 972, 1044, 1059, 1199
Zsp2I ATGCAT 2 cut(s) 214, 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.