Rw5G027960

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000080
Physical Location & Seq
Forward (+)
41758297 .. 41765465
7169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027960.1

Sequence Viewer

Length: 2937 bp
ATGGAGCGTGTGCGTGCTCGGCACCGACAATGGCAAGAGTCAATGAGTTCTTCACAATGTCAAGCAAACTTGGCACGACGACGCATGATAGCTCAAGAAAAGAATTTCCTCCAAAAAAATGCTAATTCATTTGTGTTAATAGAAGAATTTGACAATCAAGGCACAAGTTCAAGAATTTCTTCAAATTCCAACAACCCCGAACAAGACTACTCAAGGGTTCGCTTAACTCGCATAAGACAAATGGCACGTTCGAATAGATCAGAAACAAGCGATGGAGTTGTCCGAATTTCTACTCGCTATGGTGAGCTTAATATATTAAACTTATTGATAGCGAATCATTTACCTAATGCTACCATGAATAATCATGAGGAGCATAACAACAACAATGTGGCCATGAATAATGATGAGGAACATACCAACAACATTGAGGAAGACAATGAAAGTGAGCCCATGAATAATCATGAGGAGCGCCAGACCTTTAGTTTGTGTTGCTTGAAGGGGAATATTGTTCTACCACTTATACAGTCTCCCCCAGAAATGGTTCACCTCTTTTCTAATCAAACTGATGAGGGCAGAAAATTCAGACAAAATATTAGGGCCTACAACAATGGATTTCACACTATATTCGTCGCATTTATGCTCCTATCAATTGGTGTACATGTTGATGAAAGAATTAATATCCAAGGCCGTGGACCTTATACGTTTCGTGCACAAGGTTCTATATATCATAAAATTGGTGATCTGTTACCAATTGACGAGAGAAGACCACGATATCTACAGGCTTATATATATGACACTGAGAATGAACTTGAGAATAGATTGTTTGAAAATGAAGTTTTGGAGAGGGGAATGGTTGAAAAGATACAACAAATTCTCAACCAACACAATCCATTTGTTCAAACATTTCAAAGTCTCGGACAACGTCAAGATTTGCCCAATTGCAGACTCATCATCAGAGAGCAGCCTTCCGACCACCGTCAGTATAATTTGCCATCTACATCACAAGTTTCTGCTATAATAGTAGGGGTGACAACATCAATCCAAATGGGAGAGATTTTATTGTTCAAACAATTAGTAGACAACTTTGGAATGTCAAAGACTCAGTTGGATATTATGACCCCATGCAGTATCCACATTAATACTCATGGCAATAATGGAAGACCAATGTCATGTCATGAAAATACTCTACATCGAGGAGGACGCCTCTTACAACAGTACGTTGTAGATAATTATGTAAAGATTGAATCACAGAAGCTCAGATGGCTTAGTCGTAATCAAGAAAAATTTCGGAGAGAATTTAGTCAACGACTTCAAGACTCTCTACATGCAGGAGAAAATGATGCAGAAGAAAATGACAAGCTTAATAGCCCTGATGAATATGATCGCATTGTTAGAGCTGAAATACCATATGAGGACAAGGAGCCTCAACTTTATGATGCAGTATGTACACATATGATTCATGGTCCATGTGGAACTCTTAACCCAAGGCAGTCATGTATGAAAAATGGGAGTTGCAATAAAGGCTACCCAAAACCATTTGCAAATTTCACTGTGCAAGGGAATGATGCATATCTTGTATATCGGAGACTAGCAAGTCGTTTACCGGTACCTTCGCGTAGACGAGGAGATGTGATGGTTGATAACAGTTGGGTGGTTCCCTACAACCCATGGTTGCTATTACGGTATAATTGTCACATCAATGTTGAAATATGTGGGAGCATAAATAGTGCCAAGTACTTATATAAGTATATCTATAAAGGTCCAGGCAGATTTGATGAAATTAGACAATTTGTTGATGCAAGGTGGGTCTGTGCACCAGAGGGTTTATGGAGGATCTTCAAATTTGTAATGAACAAAATATATCCAACAGTAGAGCGATTACAAATACATCTTCCCAACATGCAACAGATAACATTCAATGTTGATGAAACAGTAGAGAATATTATGGTAGATGAGCACGCACAAATGTCAATGCTTACTGAAGCATATTTGTATCGAGAAATTCCTGAACATTACAGGTGGGATAATAGCAACAAAATTTGGGTGAAAAGAAGGAGAAACTACAAGGTTATTGGGAGAATATACAGTATCTCACCGTATGAAGGTGAGAAATTTTACCTGCGTGTCTTTCTTGATCATTTCAGAGGTCCAGGATCATTTCATGATTTGCTTACTGTTAATGGTGTTCTACAACCAACTTTCAAGCAAGCAGCAGAGAAACAATGTTTGTTTGAGGCCTCTGCAATTAGGATGCCATCAACTTTGATGAGATTATTCGTCACAATTTTGGTGTACAATGAGCCAATTGGTGTCCGATCATTGTGGGATGAATTTTTTCCTTTTATGGTGGAAAATTATCCTTCATCTAGTGATATTGCAAACCGAAACAGGCTCTTACAAGATTTAAATGGAGTACTTGAACAATTTAACAGGAACATTAGAGATTTTGACCTGCCTGAAATGACAATGGAGCCAAATGAAAATTCAAGAATGCCAAGGTGTATTGAAGACGAACTTTCCGTACATATCTCTCAAGAATACATTGATGCAATTAACTGCCTTAATGATGACCAAAGGATCGCCTTTAACATGATCATGGATGCAGTTCAGAGAAAAAGAAGTGCATTATTTTTTGTTGATGGTCCTGGTGGAACTAGAAAGACATATTTATACATCTTAGCCACAACATCATCTGGAATAGCTGCAAATATACTGCCCGGCGGGAGGACAGCTCACTCTAGATTTAAGATTCCACTCAAAGTAGATGCGTCATCTATGTGTTCCATAAGTAAACAGTCTTCTCTGGCACAACTGATACAAGATTCGACTGCAATTATTTGGGATGAGGCACCTATGACACATCGACATAAGTTTTGCTTCATTCAAACACAAATGATAGATCGTCTTCATAGGTATTTTGACATTTTTATTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

978

Amino Acids

114.01

Weight (kDa)

7.02

Isoelectric Point (pI)

56.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 394 - 456 8.3e-08 Helitron helicase-like domain at N-terminus
PIF1 PF05970 853 - 962 9.1e-32 PIF1-like helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1593
Acc36I ACCTGC 2 cut(s) 2127, 2460
Acc65I GGTACC 1 cut(s) 1606
AccB1I GGYRCC 3 cut(s) 21, 1606, 2848
AccI GTMKAC 2 cut(s) 1077, 1618
AccII CGCG 1 cut(s) 1615
AciI CCGC 1 cut(s) 2721
AclWI GGATC 3 cut(s) 1841, 2161, 2585
AcoI YGGCCR 1 cut(s) 390
AcuI CTGAAG 1 cut(s) 2001
AcyI GRCGYC 1 cut(s) 1201
AfaI GTAC 8 cut(s) 657, 1217, 1447, 1608, 1736, 2294, 2415, 2523
AfiI CCNNNNNNNGG 3 cut(s) 538, 2102, 2724
AflIII ACRYGT 1 cut(s) 658
AgeI ACCGGT 1 cut(s) 1603
AjnI CCWGG 3 cut(s) 1762, 2149, 2644
AjuI GAANNNNNNNTTGG 2 cut(s) 2467, 2499
AluBI AGCT 7 cut(s) 92, 307, 1255, 1360, 1397, 2702, 2732
AluI AGCT 7 cut(s) 92, 307, 1255, 1360, 1397, 2702, 2732
Alw21I GWGCWC 4 cut(s) 19, 712, 1816, 1959
Alw26I GTCTC 3 cut(s) 531, 917, 1579
Alw44I GTGCAC 2 cut(s) 708, 1812
AlwI GGATC 3 cut(s) 1841, 2161, 2585
AoxI GGCC 4 cut(s) 390, 597, 685, 2235
ApaLI GTGCAC 2 cut(s) 708, 1812
ApeKI GCWGC 3 cut(s) 961, 2210, 2702
ArsI GACNNNNNNTTYG 4 cut(s) 466, 498, 2856, 2888
AseI ATTAAT 2 cut(s) 675, 1137
AsiGI ACCGGT 1 cut(s) 1603
Asp700I GAANNNNTTC 4 cut(s) 178, 540, 1284, 2334
Asp718I GGTACC 1 cut(s) 1606
AspLEI GCGC 1 cut(s) 471
AspS9I GGNCC 6 cut(s) 597, 692, 1463, 1760, 2147, 2642
AsuC2I CCSGG 1 cut(s) 2718
AsuHPI GGTGA 7 cut(s) 314, 536, 749, 1039, 2056, 2085, 2117
AsuII TTCGAA 1 cut(s) 251
AvaII GGWCC 5 cut(s) 692, 1463, 1760, 2147, 2642
BaeGI GKGCMC 2 cut(s) 712, 1816
BalI TGGCCA 1 cut(s) 392
BanI GGYRCC 3 cut(s) 21, 1606, 2848
BanII GRGCYC 1 cut(s) 450
BarI GAAGNNNNNNTAC 2 cut(s) 2782, 2814
BbsI GAAGAC 6 cut(s) 438, 769, 1165, 2514, 2790, 2897
Bbv12I GWGCWC 4 cut(s) 19, 712, 1816, 1959
BbvI GCAGC 3 cut(s) 973, 2222, 2689
BccI CCATC 6 cut(s) 266, 1000, 1254, 1627, 2263, 2633
BceAI ACGGC 1 cut(s) 672
BciT130I CCWGG 3 cut(s) 1764, 2151, 2646
BciVI GTATCC 1 cut(s) 1139
BclI TGATCA 2 cut(s) 2134, 2592
BcnI CCSGG 1 cut(s) 2718
BcoDI GTCTC 3 cut(s) 531, 917, 1579
BfaI CTAG 4 cut(s) 1589, 2367, 2655, 2739
BfmI CTRYAG 1 cut(s) 776
BfoI RGCGCY 1 cut(s) 472
BfuAI ACCTGC 2 cut(s) 2127, 2460
BfuI GTATCC 1 cut(s) 1139
BisI GCNGC 3 cut(s) 962, 2211, 2703
BlsI GCNGC 3 cut(s) 963, 2212, 2704
BmcAI AGTACT 2 cut(s) 1736, 2415
Bme1390I CCNGG 4 cut(s) 1764, 2151, 2646, 2718
Bme18I GGWCC 5 cut(s) 692, 1463, 1760, 2147, 2642
BmgT120I GGNCC 6 cut(s) 597, 692, 1463, 1760, 2147, 2642
BmiI GGNNCC 6 cut(s) 23, 1422, 1608, 1656, 2472, 2850
BmrFI CCNGG 4 cut(s) 1764, 2151, 2646, 2718
BmsI GCATC 8 cut(s) 1330, 1426, 1555, 1786, 2241, 2536, 2590, 2755
BoxI GACNNNNGTC 2 cut(s) 975, 1165
BpiI GAAGAC 6 cut(s) 438, 769, 1165, 2514, 2790, 2897
BplI GAGNNNNNCTC 4 cut(s) 1188, 1220, 2716, 2748
Bpu14I TTCGAA 1 cut(s) 251
BpuEI CTTGAG 4 cut(s) 78, 196, 830, 2517
BpuMI CCSGG 1 cut(s) 2718
BsaBI GATNNNNATC 1 cut(s) 1569
BsaHI GRCGYC 1 cut(s) 1201
BsaJI CCNNGG 5 cut(s) 682, 688, 1484, 1667, 2495
BsaWI WCCGGW 1 cut(s) 1603
Bsc4I CCNNNNNNNGG 3 cut(s) 538, 2102, 2724
Bse118I RCCGGY 1 cut(s) 1603
Bse8I GATNNNNATC 1 cut(s) 1569
BseBI CCWGG 3 cut(s) 1764, 2151, 2646
BseDI CCNNGG 5 cut(s) 682, 688, 1484, 1667, 2495
BseGI GGATG 4 cut(s) 2256, 2332, 2605, 2848
BseJI GATNNNNATC 1 cut(s) 1569
BseLI CCNNNNNNNGG 3 cut(s) 538, 2102, 2724
BseMII CTCAG 3 cut(s) 789, 1115, 1270
BseRI GAGGAG 4 cut(s) 383, 479, 1209, 1638
BseSI GKGCMC 2 cut(s) 712, 1816
BseXI GCAGC 3 cut(s) 973, 2222, 2689
Bsh1236I CGCG 1 cut(s) 1615
BshFI GGCC 4 cut(s) 392, 599, 687, 2237
BshNI GGYRCC 3 cut(s) 21, 1606, 2848
BshTI ACCGGT 1 cut(s) 1603
BsiHKAI GWGCWC 4 cut(s) 19, 712, 1816, 1959
BsiSI CCGG 2 cut(s) 1604, 2718
BslI CCNNNNNNNGG 3 cut(s) 538, 2102, 2724
BsmAI GTCTC 3 cut(s) 531, 917, 1579
BsmI GAATGC 1 cut(s) 2496
BsnI GGCC 4 cut(s) 392, 599, 687, 2237
Bsp119I TTCGAA 1 cut(s) 251
Bsp1286I GDGCHC 5 cut(s) 19, 450, 712, 1816, 1959
Bsp1407I TGTACA 3 cut(s) 655, 1445, 2292
Bsp19I CCATGG 1 cut(s) 1667
BspACI CCGC 1 cut(s) 2721
BspANI GGCC 4 cut(s) 392, 599, 687, 2237
BspCNI CTCAG 3 cut(s) 790, 1114, 1269
BspFNI CGCG 1 cut(s) 1615
BspHI TCATGA 4 cut(s) 364, 460, 1174, 2161
BspLI GGNNCC 6 cut(s) 23, 1422, 1608, 1656, 2472, 2850
BspMI ACCTGC 2 cut(s) 2127, 2460
BspPI GGATC 3 cut(s) 1841, 2161, 2585
BspT104I TTCGAA 1 cut(s) 251
BspT107I GGYRCC 3 cut(s) 21, 1606, 2848
BsrFI RCCGGY 1 cut(s) 1603
BsrGI TGTACA 3 cut(s) 655, 1445, 2292
BssAI RCCGGY 1 cut(s) 1603
BssECI CCNNGG 5 cut(s) 682, 688, 1484, 1667, 2495
BssNI GRCGYC 1 cut(s) 1201
BssT1I CCWWGG 4 cut(s) 682, 1484, 1667, 2495
Bst2UI CCWGG 3 cut(s) 1764, 2151, 2646
BstACI GRCGYC 1 cut(s) 1201
BstAUI TGTACA 3 cut(s) 655, 1445, 2292
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 3 cut(s) 15, 1959, 2208
BstDEI CTNAG 5 cut(s) 798, 1101, 1256, 1265, 2677
BstDSI CCRYGG 2 cut(s) 688, 1667
BstF5I GGATG 4 cut(s) 2256, 2332, 2605, 2848
BstFNI CGCG 1 cut(s) 1615
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 1 cut(s) 471
BstMAI GTCTC 3 cut(s) 531, 917, 1579
BstMWI GCNNNNNNNGC 5 cut(s) 19, 71, 228, 1261, 1521
BstNI CCWGG 3 cut(s) 1764, 2151, 2646
BstNSI RCATGY 3 cut(s) 662, 1328, 1903
BstPAI GACNNNNGTC 2 cut(s) 975, 1165
BstSCI CCNGG 4 cut(s) 1762, 2149, 2644, 2716
BstSFI CTRYAG 1 cut(s) 776
BstSLI GKGCMC 2 cut(s) 712, 1816
BstUI CGCG 1 cut(s) 1615
BstV1I GCAGC 3 cut(s) 973, 2222, 2689
BstV2I GAAGAC 6 cut(s) 438, 769, 1165, 2514, 2790, 2897
BstX2I RGATCY 1 cut(s) 1833
BstXI CCANNNNNNTGG 1 cut(s) 689
BstYI RGATCY 1 cut(s) 1833
BsuI GTATCC 1 cut(s) 1139
BsuRI GGCC 4 cut(s) 392, 599, 687, 2237
BtgI CCRYGG 2 cut(s) 688, 1667
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 4 cut(s) 2256, 2332, 2605, 2848
BtsIMutI CAGTG 2 cut(s) 795, 1548
BveI ACCTGC 2 cut(s) 2127, 2460
Cac8I GCNNGC 3 cut(s) 15, 1959, 2208
CciI TCATGA 4 cut(s) 364, 460, 1174, 2161
CfoI GCGC 1 cut(s) 471
Cfr10I RCCGGY 1 cut(s) 1603
Cfr13I GGNCC 6 cut(s) 597, 692, 1463, 1760, 2147, 2642
CseI GACGC 3 cut(s) 90, 1209, 2757
Csp6I GTAC 8 cut(s) 656, 1216, 1446, 1607, 1735, 2293, 2414, 2522
CspAI ACCGGT 1 cut(s) 1603
CviQI GTAC 8 cut(s) 656, 1216, 1446, 1607, 1735, 2293, 2414, 2522
DdeI CTNAG 5 cut(s) 798, 1101, 1256, 1265, 2677
DraI TTTAAA 1 cut(s) 2406
DrdI GACNNNNNNGTC 1 cut(s) 1593
DseDI GACNNNNNNGTC 1 cut(s) 1593
EaeI YGGCCR 1 cut(s) 390
Eco130I CCWWGG 4 cut(s) 682, 1484, 1667, 2495
Eco147I AGGCCT 1 cut(s) 2237
Eco24I GRGCYC 1 cut(s) 450
Eco32I GATATC 1 cut(s) 773
Eco47I GGWCC 5 cut(s) 692, 1463, 1760, 2147, 2642
Eco57I CTGAAG 1 cut(s) 2001
EcoO109I RGGNCCY 1 cut(s) 597
EcoRII CCWGG 3 cut(s) 1762, 2149, 2644
EcoRV GATATC 1 cut(s) 773
EcoT14I CCWWGG 4 cut(s) 682, 1484, 1667, 2495
EcoT22I ATGCAT 1 cut(s) 1570
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 4 cut(s) 682, 1484, 1667, 2495
FalI AAGNNNNNCTT 6 cut(s) 163, 195, 2499, 2531, 2861, 2893
FauI CCCGC 1 cut(s) 2714
FauNDI CATATG 2 cut(s) 1408, 1452
FbaI TGATCA 2 cut(s) 2134, 2592
FblI GTMKAC 2 cut(s) 1077, 1618
Fnu4HI GCNGC 3 cut(s) 962, 2211, 2703
FokI GGATG 4 cut(s) 2263, 2339, 2612, 2855
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 3 cut(s) 962, 2211, 2703
FspBI CTAG 4 cut(s) 1589, 2367, 2655, 2739
GlaI GCGC 1 cut(s) 470
GluI GCNGC 3 cut(s) 962, 2211, 2703
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 4 cut(s) 392, 599, 687, 2237
HapII CCGG 2 cut(s) 1604, 2718
HgaI GACGC 3 cut(s) 90, 1209, 2757
HhaI GCGC 1 cut(s) 471
Hin1I GRCGYC 1 cut(s) 1201
Hin6I GCGC 1 cut(s) 469
HinP1I GCGC 1 cut(s) 469
HincII GTYRAC 1 cut(s) 1304
HindII GTYRAC 1 cut(s) 1304
HindIII AAGCTT 1 cut(s) 1358
HinfI GANTC 9 cut(s) 38, 334, 945, 1099, 1244, 1316, 1456, 2749, 2822
HpaII CCGG 2 cut(s) 1604, 2718
HphI GGTGA 7 cut(s) 314, 536, 749, 1039, 2056, 2085, 2117
Hpy99I CGWCG 3 cut(s) 81, 84, 632
HpyAV CCTTC 6 cut(s) 490, 975, 1620, 2046, 2096, 2370
HpyCH4IV ACGT 4 cut(s) 247, 701, 922, 1218
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 71, 228, 1261, 1521
HpyF3I CTNAG 5 cut(s) 798, 1101, 1256, 1265, 2677
HpySE526I ACGT 4 cut(s) 247, 701, 922, 1218
Hsp92I GRCGYC 1 cut(s) 1201
HspAI GCGC 1 cut(s) 469
KpnI GGTACC 1 cut(s) 1610
Ksp22I TGATCA 2 cut(s) 2134, 2592
LmnI GCTCC 7 cut(s) 4, 370, 466, 645, 1420, 1716, 2470
Lsp1109I GCAGC 3 cut(s) 973, 2222, 2689
LweI GCATC 8 cut(s) 1330, 1426, 1555, 1786, 2241, 2536, 2590, 2755
MaeI CTAG 4 cut(s) 1589, 2367, 2655, 2739
MaeII ACGT 4 cut(s) 247, 701, 922, 1218
MaeIII GTNAC 4 cut(s) 744, 1027, 1691, 2278
MfeI CAATTG 4 cut(s) 648, 750, 937, 2304
MflI RGATCY 1 cut(s) 1833
MhlI GDGCHC 5 cut(s) 19, 450, 712, 1816, 1959
MlsI TGGCCA 1 cut(s) 392
MluNI TGGCCA 1 cut(s) 392
MlyI GAGTC 4 cut(s) 47, 939, 1093, 1310
MmeI TCCRAC 4 cut(s) 213, 993, 1086, 1889
Mox20I TGGCCA 1 cut(s) 392
Mph1103I ATGCAT 1 cut(s) 1570
MroXI GAANNNNTTC 4 cut(s) 178, 540, 1284, 2334
MscI TGGCCA 1 cut(s) 392
MslI CAYNNNNRTG 4 cut(s) 663, 1698, 2594, 2776
Msp20I TGGCCA 1 cut(s) 392
MspI CCGG 2 cut(s) 1604, 2718
MspR9I CCNGG 4 cut(s) 1764, 2151, 2646, 2718
MunI CAATTG 4 cut(s) 648, 750, 937, 2304
Mva1269I GAATGC 1 cut(s) 2496
MvaI CCWGG 3 cut(s) 1764, 2151, 2646
MvnI CGCG 1 cut(s) 1615
MwoI GCNNNNNNNGC 5 cut(s) 19, 71, 228, 1261, 1521
NciI CCSGG 1 cut(s) 2718
NcoI CCATGG 1 cut(s) 1667
NdeI CATATG 2 cut(s) 1408, 1452
NlaIV GGNNCC 6 cut(s) 23, 1422, 1608, 1656, 2472, 2850
NmuCI GTSAC 3 cut(s) 1027, 1691, 2278
NsiI ATGCAT 1 cut(s) 1570
NspI RCATGY 3 cut(s) 662, 1328, 1903
NspV TTCGAA 1 cut(s) 251
PagI TCATGA 4 cut(s) 364, 460, 1174, 2161
PceI AGGCCT 1 cut(s) 2237
PciI ACATGT 1 cut(s) 658
PcsI WCGNNNNNNNCGW 1 cut(s) 2517
PctI GAATGC 1 cut(s) 2496
PdmI GAANNNNTTC 4 cut(s) 178, 540, 1284, 2334
PfeI GAWTC 5 cut(s) 334, 1244, 1456, 2749, 2822
PflFI GACNNNGTC 1 cut(s) 921
PfoI TCCNGGA 1 cut(s) 2149
PinAI ACCGGT 1 cut(s) 1603
PkrI GCNGC 3 cut(s) 963, 2212, 2704
PleI GAGTC 4 cut(s) 46, 939, 1093, 1310
PpsI GAGTC 4 cut(s) 46, 939, 1093, 1310
PscI ACATGT 1 cut(s) 658
PshAI GACNNNNGTC 2 cut(s) 975, 1165
PshBI ATTAAT 2 cut(s) 675, 1137
Psp6I CCWGG 3 cut(s) 1762, 2149, 2644
PspGI CCWGG 3 cut(s) 1762, 2149, 2644
PspN4I GGNNCC 6 cut(s) 23, 1422, 1608, 1656, 2472, 2850
PspPI GGNCC 6 cut(s) 597, 692, 1463, 1760, 2147, 2642
PsrI GAACNNNNNNTAC 2 cut(s) 2505, 2537
PsuI RGATCY 1 cut(s) 1833
PsyI GACNNNGTC 1 cut(s) 921
RsaI GTAC 8 cut(s) 657, 1217, 1447, 1608, 1736, 2294, 2415, 2523
RsaNI GTAC 8 cut(s) 656, 1216, 1446, 1607, 1735, 2293, 2414, 2522
RseI CAYNNNNRTG 4 cut(s) 663, 1698, 2594, 2776
SatI GCNGC 3 cut(s) 962, 2211, 2703
Sau96I GGNCC 6 cut(s) 597, 692, 1463, 1760, 2147, 2642
ScaI AGTACT 2 cut(s) 1736, 2415
SchI GAGTC 4 cut(s) 47, 939, 1093, 1310
ScrFI CCNGG 4 cut(s) 1764, 2151, 2646, 2718
SduI GDGCHC 5 cut(s) 19, 450, 712, 1816, 1959
SfaNI GCATC 8 cut(s) 1330, 1426, 1555, 1786, 2241, 2536, 2590, 2755
SfcI CTRYAG 1 cut(s) 776
SfuI TTCGAA 1 cut(s) 251
SinI GGWCC 5 cut(s) 692, 1463, 1760, 2147, 2642
SmiI ATTTAAAT 1 cut(s) 2406
SmiMI CAYNNNNRTG 4 cut(s) 663, 1698, 2594, 2776
SmlI CTYRAG 4 cut(s) 93, 211, 809, 2532
SmoI CTYRAG 4 cut(s) 93, 211, 809, 2532
SseBI AGGCCT 1 cut(s) 2237
SsiI CCGC 1 cut(s) 2721
SspI AATATT 3 cut(s) 505, 592, 1942
SspMI CTAG 4 cut(s) 1589, 2367, 2655, 2739
StuI AGGCCT 1 cut(s) 2237
StyD4I CCNGG 4 cut(s) 1762, 2149, 2644, 2716
StyI CCWWGG 4 cut(s) 682, 1484, 1667, 2495
SwaI ATTTAAAT 1 cut(s) 2406
TaiI ACGT 4 cut(s) 250, 704, 925, 1221
TaqI TCGA 5 cut(s) 251, 1192, 1996, 2825, 2863
TatI WGTACW 5 cut(s) 655, 1445, 1734, 2292, 2413
TfiI GAWTC 5 cut(s) 334, 1244, 1456, 2749, 2822
TscAI CASTG 2 cut(s) 802, 1555
TseFI GTSAC 3 cut(s) 1027, 1691, 2278
TseI GCWGC 3 cut(s) 961, 2210, 2702
Tsp45I GTSAC 3 cut(s) 1027, 1691, 2278
TspGWI ACGGA 1 cut(s) 2509
TspRI CASTG 2 cut(s) 802, 1555
Tth111I GACNNNGTC 1 cut(s) 921
VneI GTGCAC 2 cut(s) 708, 1812
VpaK11BI GGWCC 5 cut(s) 692, 1463, 1760, 2147, 2642
VspI ATTAAT 2 cut(s) 675, 1137
XbaI TCTAGA 1 cut(s) 2738
XceI RCATGY 3 cut(s) 662, 1328, 1903
XcmI CCANNNNNNNNNTGG 1 cut(s) 1824
XmiI GTMKAC 2 cut(s) 1077, 1618
XmnI GAANNNNTTC 4 cut(s) 178, 540, 1284, 2334
XspI CTAG 4 cut(s) 1589, 2367, 2655, 2739
ZrmI AGTACT 2 cut(s) 1736, 2415
Zsp2I ATGCAT 1 cut(s) 1570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.