Rw1G021180

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000080
Physical Location & Seq
Reverse (-)
45270213 .. 45273200
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021180.1

Sequence Viewer

Length: 2925 bp
ATGGCTTTGGTCCAAAAATATGGAAAGCCGGATTTTTTCCTCACAATGACATGTAATCCAAATTGGGAAGAAATAACTGCAGAGTTATTACCTGGTCAAGCGGCACAAGATCGCCCGGATCTAACTACGCGAATTTTTCACGCCAAATTTGAAGAGTTAAAGAATGATGTTATTAAAAAAGGAGTCTTGGGTACTGTGGTAACATACGTATATGTCATCGAGTTTCAAAAACGTGGTCTCCCACACGTTTGTATGCTTCTCATGTTAAGTGATGATGATAAACTAAACAACCCAGATGATTATAACCACATTGTTCGGGCTGAAATTTCAGATCAAAATGAAGAGCCAGAGTTATACAATGCGGTGTTGGGGCACATGATACACGGTCCATGTGGGGTACATAGGCAAACTGCTCCATGTATGAAGCGTGGGAGCTGCAAACGAGGCTATCCAAAGCCATTTTCAGCAAACACATTTCAAGGAAATGATTCTTATCCCGTTTATCGTAGACGAGACAATCACTCAGATATGCAATCTAATGGCAATCAAAATAGACCACTGGATAACAGTTGGGTTATCCCCTATAACCCATGGTTACTCACAAAATATGATTGTCATATCAATGTGGAAATCTGTTGCAGCATTAAGAGTGTCAAATATTTGTATAAATATGTCTATAAAGGTCCAGACAGAGTAATATTTGAAGTTCAACCTGAAGCAAATCAAGATGAAATAAGAAACTTTGTTGATGCAAGATGGGTTTGTGCACCAGAAGCTTTATGGAGGATGTTTAAATTCGTGATGAATCGAATGTATCCATCAGTGGAACGATTGCAAATTCATCTTCCAGATGGACACAATGTTTTCTTTAATGCCACTGAAAGTAATACTTATTTTGAAGGTGCGCGTCAATACTTATATCGAGAATTTCCTGAGCATTTCAGATGGGATGGGAGACATAAAACATGGGAAGCAAGGGAGACCAACCAAAAAGTCATTGGAAGAATTTACACAGTGTCACCTTCAGAAGGAGAAAAATTTTACTTGCGTGTTCTTCTTAATCATGTTCGAGGACCTAAGTCATTTGAGGATTTAAGGACATTTGATGGTGTCTTACAACCGACATTCAAGAAAGCAGTTGAACAACGTGGTTTGTTAGAAGATGATGAAAGTATTAGACAATGTCTACTTGAGGCCTCTAATAATATTCGTATGCCGTCAAATTTAAGAAGATTGTTTGTCACCATCCTAGTTTATTGCATGCCTCATGGTGTACGCAGTTTGTGGGATGAATTTTATCCATTCATGATAGAGGATTATCCTTCTTCGAGCACCACAGACAATGTACGTATCACAAATAGACTTTTACGAGACTTTAACCAGTTGCTGGTGCAACACAGTAAGTCTGTCTTTGATTATGACTTGCCAGAAATGACTCAAGATGGTGGTGAAAATTCTTCAATGCTGAGGCTAATACAAGATGAAATTTCAATTAACATTCCTCAAGAAGACTGTGATGCAGTTCATCATTTGAATGAGGATCAAACTTTTGCATACAATTCCATAATATCTGCTATTGAGTGTCATGACAATGCCATATATTTTGTTGATGGTCTAGGAGGAACTGGAAAGACATACTTATATCGTGCATTATTAGCAAGCTTGAGAAGCAATGATCATATTGTATTGGCAACAACAACTTCTGGCATAGCAGCAACAATACTACCTGGTGGGAGGACAACACACTCTAGGTTTAAAATTCCTCTTAATCTTGATGCATCTTCGACATGCTCCATTAGTAAACAGTCTGATTTAGCAGAGTTGATAAGAAAGTCATCGGCAATTATTTGGGATGAAGCACCAATGATGAATCGATATGGGTTTGAAGCTCTTGATCGAACATTTAGAGACATAACAAGTGTCGATTTGCCATTTGGAGGAAAGGTGATGATCTTCGGGGGCGATTTCCGACAAGTTCTTCCTGTTATCCATAAGGGTACGAGATCTGAGATGGTGCAAGCCAGTTTGATTAATGCTTCATTTTGGAAGGATGTGAAGATTCTACGTTTGACACAAAATATGAGATCCATTAATGATCCAAAGTTCTCAGAGTTCTTACTTCGTGTTGGTAATGCAGAACAACCAACTGTCGTTGAAGACATAATACAAATACCTTCGCCCATGGTCATATCATGGGAGAATGAAGAATCAATCAATCAGTTGGTTAATCAAGTCTTCCCTAATTTGGATCGTCATGTAAATGATTCCAAGTACATGGTTGAAAGGGCAATAATTACTCCAAAAAATGATGATGTGGACGTACTCAATGAAAGAATCATACAACAATTTCCGGGGCCTGAACGAATCTTATACTCTTTTGACTCAGTTGAGGATGACACCAGAAATATGTATCAACAAGAATTTTTAAATTCGATTTCACCTTCTGGTATGCCGCCACATCAGTTGATTATAAAGAAAGGTGCCCCAATTATGCTTTTGATAAATATTGACCCAAAGATGGGGTTGTGCAACGGTACAAGATTGACTTGCCGAGGAGCATATAACAATCTAATTGATGCAGAGATTTTAACTGGACAATTTTCGGGAACTAGAGTTTTCTTGCCAAGAATTGCTCTTAAGAGTGCCGAAAGTTCTGGACTTCCTTTTGAGATGACAAGAAAGAAGTTTCCCGTAAAGTTGAGTTTTGCACTTACCATAAATAAATCACAAGGGCAGACAATACCAAATGTTGGTATTTACCTTCCTGATCATGTGTTTAGCCATGGACAACTGTACGTGGCTTTATCGAGAGGAGTTTCAGAGGCAAGCACCAAAGTTTTGGTAAAAAAAGGTTCATTAGAGGGTGAGGAAGGGGTATTCACAGCAAACGTTGTGTATAAAGAAATTTTGCTTCCTTCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

974

Amino Acids

111.32

Weight (kDa)

6.13

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 1 - 87 4.1e-33 Helitron helicase-like domain at N-terminus
PIF1 PF05970 511 - 714 3e-73 PIF1-like helicase
Pif1_2B_dom PF21530 807 - 849 6.9e-18 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 303, 2471
AccB1I GGYRCC 1 cut(s) 2480
AccB7I CCANNNNNTGG 2 cut(s) 1387, 2750
AccI GTMKAC 2 cut(s) 508, 1186
AccII CGCG 2 cut(s) 130, 907
AciI CCGC 3 cut(s) 101, 362, 2453
AclI AACGTT 1 cut(s) 2889
AclWI GGATC 5 cut(s) 126, 1548, 2079, 2090, 2256
AcuI CTGAAG 2 cut(s) 735, 1008
AfaI GTAC 9 cut(s) 193, 399, 1275, 1347, 1999, 2273, 2322, 2536, 2795
AfiI CCNNNNNNNGG 7 cut(s) 1028, 1387, 1937, 2245, 2518, 2519, 2750
AflII CTTAAG 1 cut(s) 2636
AflIII ACRYGT 2 cut(s) 50, 244
AjnI CCWGG 2 cut(s) 91, 1726
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 4 cut(s) 435, 776, 1662, 1889
AluI AGCT 4 cut(s) 435, 776, 1662, 1889
Alw21I GWGCWC 2 cut(s) 769, 1334
Alw26I GTCTC 6 cut(s) 242, 507, 949, 974, 1365, 1902
Alw44I GTGCAC 1 cut(s) 765
AlwI GGATC 5 cut(s) 126, 1548, 2079, 2090, 2256
AlwNI CAGNNNCTG 1 cut(s) 1387
AoxI GGCC 2 cut(s) 1194, 2354
ApaLI GTGCAC 1 cut(s) 765
ApeKI GCWGC 3 cut(s) 435, 639, 1712
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
AseI ATTAAT 2 cut(s) 2031, 2091
Asp700I GAANNNNTTC 1 cut(s) 487
AspLEI GCGC 1 cut(s) 907
AspS9I GGNCC 5 cut(s) 10, 386, 683, 1073, 2354
AsuC2I CCSGG 2 cut(s) 116, 2352
AsuHPI GGTGA 6 cut(s) 1011, 1234, 1460, 1957, 2430, 2876
AvaII GGWCC 4 cut(s) 10, 386, 683, 1073
BaeGI GKGCMC 3 cut(s) 375, 769, 2485
BaeI ACNNNNGTAYC 2 cut(s) 183, 216
BanI GGYRCC 1 cut(s) 2480
BarI GAAGNNNNNNTAC 2 cut(s) 2148, 2180
BbsI GAAGAC 3 cut(s) 1515, 2163, 2227
Bbv12I GWGCWC 2 cut(s) 769, 1334
BbvCI CCTCAGC 1 cut(s) 1466
BbvI GCAGC 3 cut(s) 422, 651, 1724
BceAI ACGGC 1 cut(s) 1201
BciT130I CCWGG 2 cut(s) 93, 1728
BciVI GTATCC 1 cut(s) 825
BclI TGATCA 2 cut(s) 1675, 2767
BcnI CCSGG 2 cut(s) 116, 2352
BcoDI GTCTC 6 cut(s) 242, 507, 949, 974, 1365, 1902
BfaI CTAG 4 cut(s) 1250, 1616, 1749, 2610
BfmI CTRYAG 1 cut(s) 78
BfrI CTTAAG 1 cut(s) 2636
BfuI GTATCC 1 cut(s) 825
BglII AGATCT 1 cut(s) 2003
BisI GCNGC 5 cut(s) 102, 436, 640, 1713, 2453
BlsI GCNGC 5 cut(s) 103, 437, 641, 1714, 2454
Bme1390I CCNGG 4 cut(s) 93, 116, 1728, 2352
Bme18I GGWCC 4 cut(s) 10, 386, 683, 1073
BmgT120I GGNCC 5 cut(s) 10, 386, 683, 1073, 2354
BmiI GGNNCC 2 cut(s) 2355, 2482
BmrFI CCNGG 4 cut(s) 93, 116, 1728, 2352
BmsI GCATC 5 cut(s) 739, 1507, 1765, 1787, 2566
BoxI GACNNNNGTC 1 cut(s) 1078
BpiI GAAGAC 3 cut(s) 1515, 2163, 2227
Bpu10I CCTNAGC 2 cut(s) 933, 1466
BpuEI CTTGAG 4 cut(s) 1211, 1422, 1488, 1684
BpuMI CCSGG 2 cut(s) 116, 2352
Bsa29I ATCGAT 1 cut(s) 1873
BsaAI YACGTR 3 cut(s) 208, 1349, 2797
BsaBI GATNNNNATC 1 cut(s) 492
BsaI GGTCTC 2 cut(s) 242, 974
BsaJI CCNNGG 5 cut(s) 590, 2181, 2351, 2551, 2782
BsaXI ACNNNNNCTCC 4 cut(s) 222, 252, 1929, 1959
Bsc4I CCNNNNNNNGG 7 cut(s) 1028, 1387, 1937, 2245, 2518, 2519, 2750
Bse1I ACTGG 5 cut(s) 564, 1381, 1630, 2022, 2596
Bse3DI GCAATG 1 cut(s) 1678
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 2 cut(s) 93, 1728
BseCI ATCGAT 1 cut(s) 1873
BseDI CCNNGG 5 cut(s) 590, 2181, 2351, 2551, 2782
BseGI GGATG 7 cut(s) 792, 955, 1245, 1294, 1858, 2056, 2398
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 7 cut(s) 1028, 1387, 1937, 2245, 2518, 2519, 2750
BseMI GCAATG 1 cut(s) 1678
BseMII CTCAG 6 cut(s) 537, 924, 1457, 1998, 2121, 2397
BseNI ACTGG 5 cut(s) 564, 1381, 1630, 2022, 2596
BseRI GAGGAG 2 cut(s) 2568, 2826
BseSI GKGCMC 3 cut(s) 375, 769, 2485
BseXI GCAGC 3 cut(s) 422, 651, 1724
Bsh1236I CGCG 2 cut(s) 130, 907
BshFI GGCC 2 cut(s) 1196, 2356
BshNI GGYRCC 1 cut(s) 2480
BshVI ATCGAT 1 cut(s) 1873
BsiHKAI GWGCWC 2 cut(s) 769, 1334
BsiSI CCGG 3 cut(s) 29, 116, 2351
BslI CCNNNNNNNGG 7 cut(s) 1028, 1387, 1937, 2245, 2518, 2519, 2750
BsmAI GTCTC 6 cut(s) 242, 507, 949, 974, 1365, 1902
BsnI GGCC 2 cut(s) 1196, 2356
Bso31I GGTCTC 2 cut(s) 242, 974
Bsp1286I GDGCHC 4 cut(s) 375, 769, 1334, 2485
Bsp19I CCATGG 3 cut(s) 590, 2181, 2782
BspACI CCGC 3 cut(s) 101, 362, 2453
BspANI GGCC 2 cut(s) 1196, 2356
BspCNI CTCAG 6 cut(s) 536, 925, 1458, 1999, 2120, 2396
BspDI ATCGAT 1 cut(s) 1873
BspFNI CGCG 2 cut(s) 130, 907
BspHI TCATGA 2 cut(s) 1305, 1585
BspLI GGNNCC 2 cut(s) 2355, 2482
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 5 cut(s) 126, 1548, 2079, 2090, 2256
BspQI GCTCTTC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 2480
BspTI CTTAAG 1 cut(s) 2636
BspTNI GGTCTC 2 cut(s) 242, 974
BsrDI GCAATG 1 cut(s) 1678
BsrI ACTGG 5 cut(s) 564, 1381, 1630, 2022, 2596
BssECI CCNNGG 5 cut(s) 590, 2181, 2351, 2551, 2782
BssT1I CCWWGG 3 cut(s) 590, 2181, 2782
Bst2UI CCWGG 2 cut(s) 93, 1728
Bst6I CTCTTC 2 cut(s) 147, 336
BstAFI CTTAAG 1 cut(s) 2636
BstBAI YACGTR 3 cut(s) 208, 1349, 2797
BstC8I GCNNGC 4 cut(s) 1262, 1660, 2019, 2827
BstDEI CTNAG 7 cut(s) 523, 933, 1077, 1466, 2007, 2107, 2383
BstDSI CCRYGG 3 cut(s) 590, 2181, 2782
BstENI CCTNNNNNAGG 1 cut(s) 1026
BstF5I GGATG 7 cut(s) 792, 955, 1245, 1294, 1858, 2056, 2398
BstFNI CGCG 2 cut(s) 130, 907
BstHHI GCGC 1 cut(s) 907
BstMAI GTCTC 6 cut(s) 242, 507, 949, 974, 1365, 1902
BstMWI GCNNNNNNNGC 4 cut(s) 444, 773, 1655, 1668
BstNI CCWGG 2 cut(s) 93, 1728
BstNSI RCATGY 3 cut(s) 54, 1264, 1791
BstPAI GACNNNNGTC 1 cut(s) 1078
BstSCI CCNGG 4 cut(s) 91, 114, 1726, 2350
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 3 cut(s) 375, 769, 2485
BstSNI TACGTA 2 cut(s) 208, 1349
BstUI CGCG 2 cut(s) 130, 907
BstV1I GCAGC 3 cut(s) 422, 651, 1724
BstV2I GAAGAC 3 cut(s) 1515, 2163, 2227
BstX2I RGATCY 3 cut(s) 118, 2003, 2084
BstXI CCANNNNNNTGG 3 cut(s) 20, 2275, 2839
BstYI RGATCY 3 cut(s) 118, 2003, 2084
Bsu15I ATCGAT 1 cut(s) 1873
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 2 cut(s) 1196, 2356
BsuTUI ATCGAT 1 cut(s) 1873
BtgI CCRYGG 3 cut(s) 590, 2181, 2782
BtsCI GGATG 7 cut(s) 792, 955, 1245, 1294, 1858, 2056, 2398
BtsIMutI CAGTG 4 cut(s) 557, 828, 876, 1020
Cac8I GCNNGC 4 cut(s) 1262, 1660, 2019, 2827
CaiI CAGNNNCTG 1 cut(s) 1387
CciI TCATGA 2 cut(s) 1305, 1585
CfoI GCGC 1 cut(s) 907
Cfr13I GGNCC 5 cut(s) 10, 386, 683, 1073, 2354
ClaI ATCGAT 1 cut(s) 1873
CseI GACGC 1 cut(s) 896
CsiI ACCWGGT 2 cut(s) 91, 1726
Csp6I GTAC 9 cut(s) 192, 398, 1274, 1346, 1998, 2272, 2321, 2535, 2794
CspCI CAANNNNNGTGG 2 cut(s) 2445, 2480
CviQI GTAC 9 cut(s) 192, 398, 1274, 1346, 1998, 2272, 2321, 2535, 2794
DdeI CTNAG 7 cut(s) 523, 933, 1077, 1466, 2007, 2107, 2383
DraI TTTAAA 3 cut(s) 793, 1756, 2427
Eam1104I CTCTTC 2 cut(s) 147, 336
EarI CTCTTC 2 cut(s) 147, 336
Eco105I TACGTA 2 cut(s) 208, 1349
Eco130I CCWWGG 3 cut(s) 590, 2181, 2782
Eco147I AGGCCT 1 cut(s) 1196
Eco31I GGTCTC 2 cut(s) 242, 974
Eco47I GGWCC 4 cut(s) 10, 386, 683, 1073
Eco57I CTGAAG 2 cut(s) 735, 1008
EcoNI CCTNNNNNAGG 1 cut(s) 1026
EcoO109I RGGNCCY 2 cut(s) 1073, 2354
EcoRII CCWGG 2 cut(s) 91, 1726
EcoT14I CCWWGG 3 cut(s) 590, 2181, 2782
EcoT22I ATGCAT 1 cut(s) 1780
ErhI CCWWGG 3 cut(s) 590, 2181, 2782
FalI AAGNNNNNCTT 8 cut(s) 874, 906, 1394, 1426, 1622, 1654, 2530, 2562
FbaI TGATCA 2 cut(s) 1675, 2767
FblI GTMKAC 2 cut(s) 508, 1186
Fnu4HI GCNGC 5 cut(s) 102, 436, 640, 1713, 2453
FokI GGATG 7 cut(s) 799, 962, 1232, 1301, 1865, 2063, 2405
Fsp4HI GCNGC 5 cut(s) 102, 436, 640, 1713, 2453
FspBI CTAG 4 cut(s) 1250, 1616, 1749, 2610
GlaI GCGC 1 cut(s) 906
GluI GCNGC 5 cut(s) 102, 436, 640, 1713, 2453
HaeIII GGCC 2 cut(s) 1196, 2356
HapII CCGG 3 cut(s) 29, 116, 2351
HgaI GACGC 1 cut(s) 896
HhaI GCGC 1 cut(s) 907
Hin6I GCGC 1 cut(s) 905
HinP1I GCGC 1 cut(s) 905
HindIII AAGCTT 2 cut(s) 774, 1660
HpaII CCGG 3 cut(s) 29, 116, 2351
HphI GGTGA 6 cut(s) 1011, 1234, 1460, 1957, 2430, 2876
Hpy166II GTNNAC 6 cut(s) 509, 767, 1187, 1274, 1802, 2317
Hpy188I TCNGA 9 cut(s) 331, 526, 944, 1027, 1810, 1970, 2008, 2110, 2821
Hpy8I GTNNAC 6 cut(s) 509, 767, 1187, 1274, 1802, 2317
HpyCH4IV ACGT 9 cut(s) 207, 232, 246, 1147, 1348, 2065, 2319, 2796, 2889
HpyF10VI GCNNNNNNNGC 4 cut(s) 444, 773, 1655, 1668
HpyF3I CTNAG 7 cut(s) 523, 933, 1077, 1466, 2007, 2107, 2383
HpySE526I ACGT 9 cut(s) 207, 232, 246, 1147, 1348, 2065, 2319, 2796, 2889
HspAI GCGC 1 cut(s) 905
Ksp22I TGATCA 2 cut(s) 1675, 2767
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 4 cut(s) 418, 432, 1796, 2555
Lsp1109I GCAGC 3 cut(s) 422, 651, 1724
LweI GCATC 5 cut(s) 739, 1507, 1765, 1787, 2566
MabI ACCWGGT 2 cut(s) 91, 1726
MaeI CTAG 4 cut(s) 1250, 1616, 1749, 2610
MaeII ACGT 9 cut(s) 207, 232, 246, 1147, 1348, 2065, 2319, 2796, 2889
MaeIII GTNAC 4 cut(s) 199, 594, 1017, 1240
MflI RGATCY 3 cut(s) 118, 2003, 2084
MhlI GDGCHC 4 cut(s) 375, 769, 1334, 2485
MlyI GAGTC 3 cut(s) 192, 1429, 2375
MmeI TCCRAC 1 cut(s) 1993
Mph1103I ATGCAT 1 cut(s) 1780
MroXI GAANNNNTTC 1 cut(s) 487
MslI CAYNNNNRTG 4 cut(s) 621, 1533, 1590, 2259
MspCI CTTAAG 1 cut(s) 2636
MspI CCGG 3 cut(s) 29, 116, 2351
MspR9I CCNGG 4 cut(s) 93, 116, 1728, 2352
MvaI CCWGG 2 cut(s) 93, 1728
MvnI CGCG 2 cut(s) 130, 907
MwoI GCNNNNNNNGC 4 cut(s) 444, 773, 1655, 1668
NciI CCSGG 2 cut(s) 116, 2352
NcoI CCATGG 3 cut(s) 590, 2181, 2782
NlaIV GGNNCC 2 cut(s) 2355, 2482
NmeAIII GCCGAG 1 cut(s) 2576
NmuCI GTSAC 2 cut(s) 1017, 1240
NsiI ATGCAT 1 cut(s) 1780
NspI RCATGY 3 cut(s) 54, 1264, 1791
PaeI GCATGC 1 cut(s) 1264
PagI TCATGA 2 cut(s) 1305, 1585
PceI AGGCCT 1 cut(s) 1196
PciI ACATGT 1 cut(s) 50
PciSI GCTCTTC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 487
PfeI GAWTC 8 cut(s) 488, 805, 1870, 2059, 2207, 2264, 2334, 2364
PflFI GACNNNGTC 1 cut(s) 1182
PflMI CCANNNNNTGG 2 cut(s) 1387, 2750
PkrI GCNGC 5 cut(s) 103, 437, 641, 1714, 2454
PleI GAGTC 3 cut(s) 191, 1429, 2375
PpsI GAGTC 3 cut(s) 191, 1429, 2375
Ppu21I YACGTR 3 cut(s) 208, 1349, 2797
PpuMI RGGWCCY 1 cut(s) 1073
PscI ACATGT 1 cut(s) 50
PshAI GACNNNNGTC 1 cut(s) 1078
PshBI ATTAAT 2 cut(s) 2031, 2091
PsiI TTATAA 2 cut(s) 303, 2471
Psp1406I AACGTT 1 cut(s) 2889
Psp5II RGGWCCY 1 cut(s) 1073
Psp6I CCWGG 2 cut(s) 91, 1726
PspGI CCWGG 2 cut(s) 91, 1726
PspN4I GGNNCC 2 cut(s) 2355, 2482
PspPI GGNCC 5 cut(s) 10, 386, 683, 1073, 2354
PspPPI RGGWCCY 1 cut(s) 1073
PstI CTGCAG 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 1387
PsuI RGATCY 3 cut(s) 118, 2003, 2084
PsyI GACNNNGTC 1 cut(s) 1182
RsaI GTAC 9 cut(s) 193, 399, 1275, 1347, 1999, 2273, 2322, 2536, 2795
RsaNI GTAC 9 cut(s) 192, 398, 1274, 1346, 1998, 2272, 2321, 2535, 2794
RseI CAYNNNNRTG 4 cut(s) 621, 1533, 1590, 2259
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 5 cut(s) 102, 436, 640, 1713, 2453
Sau96I GGNCC 5 cut(s) 10, 386, 683, 1073, 2354
SchI GAGTC 3 cut(s) 192, 1429, 2375
ScrFI CCNGG 4 cut(s) 93, 116, 1728, 2352
SduI GDGCHC 4 cut(s) 375, 769, 1334, 2485
SexAI ACCWGGT 2 cut(s) 91, 1726
SfaNI GCATC 5 cut(s) 739, 1507, 1765, 1787, 2566
SfcI CTRYAG 1 cut(s) 78
SinI GGWCC 4 cut(s) 10, 386, 683, 1073
SmiMI CAYNNNNRTG 4 cut(s) 621, 1533, 1590, 2259
SmlI CTYRAG 5 cut(s) 1190, 1437, 1503, 1663, 2636
SmoI CTYRAG 5 cut(s) 1190, 1437, 1503, 1663, 2636
SnaBI TACGTA 2 cut(s) 208, 1349
SphI GCATGC 1 cut(s) 1264
SseBI AGGCCT 1 cut(s) 1196
SsiI CCGC 3 cut(s) 101, 362, 2453
SspI AATATT 4 cut(s) 659, 699, 1207, 2506
SspMI CTAG 4 cut(s) 1250, 1616, 1749, 2610
StuI AGGCCT 1 cut(s) 1196
StyD4I CCNGG 4 cut(s) 91, 114, 1726, 2350
StyI CCWWGG 3 cut(s) 590, 2181, 2782
TaiI ACGT 9 cut(s) 210, 235, 249, 1150, 1351, 2068, 2322, 2799, 2892
TatI WGTACW 1 cut(s) 2271
TauI GCSGC 2 cut(s) 104, 2455
TfiI GAWTC 8 cut(s) 488, 805, 1870, 2059, 2207, 2264, 2334, 2364
TscAI CASTG 4 cut(s) 564, 828, 883, 1020
TseFI GTSAC 2 cut(s) 1017, 1240
TseI GCWGC 3 cut(s) 435, 639, 1712
Tsp45I GTSAC 2 cut(s) 1017, 1240
TspRI CASTG 4 cut(s) 564, 828, 883, 1020
Tth111I GACNNNGTC 1 cut(s) 1182
Van91I CCANNNNNTGG 2 cut(s) 1387, 2750
Vha464I CTTAAG 1 cut(s) 2636
VneI GTGCAC 1 cut(s) 765
VpaK11BI GGWCC 4 cut(s) 10, 386, 683, 1073
VspI ATTAAT 2 cut(s) 2031, 2091
XagI CCTNNNNNAGG 1 cut(s) 1026
XceI RCATGY 3 cut(s) 54, 1264, 1791
XcmI CCANNNNNNNNNTGG 2 cut(s) 777, 995
XmiI GTMKAC 2 cut(s) 508, 1186
XmnI GAANNNNTTC 1 cut(s) 487
XspI CTAG 4 cut(s) 1250, 1616, 1749, 2610
Zsp2I ATGCAT 1 cut(s) 1780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.