Rmu_sc0003198.1_g000015

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003198.1
OG0000080
Physical Location & Seq
Forward (+)
54735 .. 57725
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003198.1_g000015.1.cds

Sequence Viewer

Length: 2991 bp
atggcgatagttcagaaatatggtaaacctgacatattgctaaccatgacgtgtaatccagcatgggaggaaatagcaaatgaattactccctggtcaaacgtcacaagaccgaccagatttgctaactcgagtttttagagcaaaatatgaacttctaaagaaagatatttacaaaactggagttcttggaaaggtcatcgctcatgtgcatgtcattgagtttcaaaaaagaggtttgccacattgtcacatggttatcattcttgatgagaatgacaaattgaacacacccgatgattatgatcacattgtaagggcggaaattccattcagagatgaagaaccggagctctacaatattgtacttaaacatatgatacatggaccatgtggaactcttaatgaaaactctccatgtatgaaaaatgggagttgcaagagaaattatccaaagcaattttctgaatttacagtgcaaggaaatgattgttaccctatatataggaggcgcaatgatggaagatctgttgtattggatagaaatactgaagtagaaattgataacagatgggttgtcccatataatccatggttgttattgaagtatgattgccatataaatttggaggtatgtagcagcataaagtgtgtgaaatatctatataagtatgtttataagggacctgatcgtgtttctttagaggtgcgcttgggaccaaactatgatgaaatatcgaagtatttagatgcaagatggatctgtgctcctgaggcattgtggaagcttttcacatttccaattaatcgtatatatccttctgttgagcgcctacaaattcatcttccaaataggcatcaagtcaggtactacagtcataccccaataagggaaattttggaagagcccacgaattctatgaccatgcttacacaattttttcaaatgaattctattagtgaatttgcaagactatggttatatagagaattccctcagcattttagatggttgggatctcaaagaacttggcagaggagagtatctacacaaaaagttattgggcgaatttatactgtgtgtccatctgaaggtgagcgtttttacttgcgtattctattaaatcatgttagaggtccaaagtcatttgatgatcttctgactgttaatggaattgcattcccaacttttaagcaagtagctgaaaggatgggtttacttgaggatgatgggagtatacgtcagtgtttattggaagtcgagtggttattgatgcagtctgctttacggagattatttgcaaccatcttggtgtattgtgaaccagttggagtccgcatattatgggaggaatttcatttatttatggcacaagactactcaacatctgcctcttcaagtaaccatgttgtcatcaacctattgttgagagacctgaatggattgctacaacaacatggtaaaagaattactgattatgatcttccccaaattgatactcagtttgcagatgacacaagaattggaagaaatatacaagatgagttgtccgtgcatgttccacccgaagacttggcctctattgatacattaaatgcagatcaacgtaatgcatttaacataatcatggcagcaattagacgtaatgaatcagcatcattcttcatagatggccccggtggaactggaaagacttatctctaccgtgcaattcttgcttcactaaggagtaatggtcatatagcaattgccacagctacgtcgggaattgcagctactattttgcctggtggaaggactgcacattcacgatttaaaattccaattgacttgctgtccacaaacgcatgctctataagtaagcaatcagatttggcagatcttctacgacgtgcggttgttcttatttgggatgaagctacaatgactcacagaaaagcttttgaggctttggatacaactcttagagatataactggtgtcgaaatgccatttggtgggaaagttatgattcttggcggagactttcggcaagttcttcctgttgtaccacatggtagcaaagcacaaatgatagatgcatgcattgtaaaatctctgttatggaatgcgatcagaatactccctttgaaacacaatatgagggcccaacaagacccggaatttgcagattttctacttcgtgtcggtgatggaaatgagccgtttgtgcaagatgatatgattaggatcccagatgcattggttattccttgggttggtgatgaatctgttggccaactaattgcttctatatttccaggtttggaggacaatgcatttgatagttcttatatggtggatagagctattataacgcctaggaatgaggatgtggacaagctaaatgaagtggtattgaattcctttccaggtgaagatcaaatttattattcttttgatagagttgaagatgaccctcacaacttatatcaacaagaattcctaaattctatagctccaggtggtttgccctctcataagttgactcttaaattgggtgcacccataatgctgcttagaaatattgatccaaaaagtgggctatgcaacgggacaaggttaatatgtcgcggattctttcctaattttattgatgcagagattctcaccggccatttcaagggtacaagagttttcctaccacgaattcctctaaagccttcagaaaatgtgaagcttccgttctctctcatacgtcggcagtttcctattatattaagctttgcactaacaataaacaaagctcaaggccaaacaattccaaatgttggtgtgtacctccctaatcacgtcttcagccatggccagctttatgtggctttatcaaggggagtatccatacaaacaaccaaggtcctaatcaagaatagtgccattgaaggagaacctggagctcacacaaggaatgttgtatttaaagatgttctaacgtcatgccaaaggcaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

996

Amino Acids

113.58

Weight (kDa)

7.15

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 678, 2354
AccB7I CCANNNNNTGG 3 cut(s) 1997, 2579, 2810
AccI GTMKAC 1 cut(s) 1237
AccII CGCG 1 cut(s) 2613
AciI CCGC 5 cut(s) 320, 1336, 1895, 2019, 2613
AclWI GGATC 5 cut(s) 767, 1024, 2224, 2237, 2564
AcoI YGGCCR 3 cut(s) 2275, 2653, 2845
AcuI CTGAAG 4 cut(s) 570, 1110, 2688, 2821
AfaI GTAC 5 cut(s) 366, 869, 2049, 2668, 2819
AfiI CCNNNNNNNGG 8 cut(s) 888, 889, 1091, 1997, 2258, 2579, 2662, 2810
AflIII ACRYGT 1 cut(s) 50
AhdI GACNNNNNGTC 1 cut(s) 1834
AjiI CACGTC 3 cut(s) 51, 1892, 2833
AjnI CCWGG 6 cut(s) 91, 1786, 2299, 2410, 2500, 2929
AjuI GAANNNNNNNTTGG 4 cut(s) 445, 477, 1977, 2009
Alw21I GWGCWC 4 cut(s) 354, 769, 2545, 2938
Alw26I GTCTC 2 cut(s) 1425, 2016
Alw44I GTGCAC 1 cut(s) 2541
AlwI GGATC 5 cut(s) 767, 1024, 2224, 2237, 2564
Ama87I CYCGRG 1 cut(s) 129
AoxI GGCC 7 cut(s) 1575, 1672, 2145, 2275, 2653, 2791, 2845
ApaI GGGCCC 1 cut(s) 2149
ApaLI GTGCAC 1 cut(s) 2541
ApeKI GCWGC 4 cut(s) 639, 1631, 1772, 2554
ArsI GACNNNNNNTTYG 4 cut(s) 2147, 2179, 2303, 2335
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 1 cut(s) 788
AspA2I CCTAGG 1 cut(s) 2360
AspLEI GCGC 3 cut(s) 513, 711, 831
AspS9I GGNCC 8 cut(s) 386, 683, 716, 1136, 1673, 2145, 2146, 2896
AsuC2I CCSGG 2 cut(s) 1677, 2159
AsuHPI GGTGA 5 cut(s) 1106, 2201, 2273, 2426, 2641
AvaI CYCGRG 1 cut(s) 129
AvaII GGWCC 5 cut(s) 386, 683, 716, 1136, 2896
AvrII CCTAGG 1 cut(s) 2360
AxyI CCTNAGG 1 cut(s) 771
BaeGI GKGCMC 2 cut(s) 2149, 2545
BaeI ACNNNNGTAYC 4 cut(s) 859, 892, 2658, 2691
BalI TGGCCA 2 cut(s) 2277, 2847
BamHI GGATCC 1 cut(s) 2229
BanII GRGCYC 4 cut(s) 354, 909, 2149, 2938
BarI GAAGNNNNNNTAC 2 cut(s) 802, 834
BbsI GAAGAC 2 cut(s) 1575, 2827
Bbv12I GWGCWC 4 cut(s) 354, 769, 2545, 2938
BbvCI CCTCAGC 1 cut(s) 996
BbvI GCAGC 4 cut(s) 651, 1643, 1784, 2541
BceAI ACGGC 1 cut(s) 2188
BciT130I CCWGG 6 cut(s) 93, 1788, 2301, 2412, 2502, 2931
BciVI GTATCC 2 cut(s) 1948, 2887
BclI TGATCA 1 cut(s) 304
BcnI CCSGG 2 cut(s) 1677, 2159
BcoDI GTCTC 2 cut(s) 1425, 2016
BfaI CTAG 1 cut(s) 2361
BfmI CTRYAG 2 cut(s) 871, 2493
BfoI RGCGCY 1 cut(s) 832
BfuI GTATCC 2 cut(s) 1948, 2887
BglII AGATCT 2 cut(s) 524, 1879
BisI GCNGC 4 cut(s) 640, 1632, 1773, 2555
BlnI CCTAGG 1 cut(s) 2360
BlsI GCNGC 4 cut(s) 641, 1633, 1774, 2556
Bme1390I CCNGG 8 cut(s) 93, 1677, 1788, 2159, 2301, 2412, 2502, 2931
Bme18I GGWCC 5 cut(s) 386, 683, 716, 1136, 2896
BmeRI GACNNNNNGTC 1 cut(s) 1834
BmeT110I CYCGRG 1 cut(s) 129
BmgBI CACGTC 3 cut(s) 51, 1892, 2833
BmgT120I GGNCC 8 cut(s) 386, 683, 716, 1136, 1673, 2145, 2146, 2896
BmiI GGNNCC 5 cut(s) 684, 717, 1675, 2147, 2231
BmrFI CCNGG 8 cut(s) 93, 1677, 1788, 2159, 2301, 2412, 2502, 2931
BmsI GCATC 7 cut(s) 739, 865, 1263, 1664, 2068, 2227, 2626
BpiI GAAGAC 2 cut(s) 1575, 2827
BpmI CTGGAG 3 cut(s) 201, 2484, 2952
Bpu10I CCTNAGC 1 cut(s) 996
BpuEI CTTGAG 2 cut(s) 1241, 2772
BpuMI CCSGG 2 cut(s) 1677, 2159
BsaBI GATNNNNATC 3 cut(s) 303, 1479, 2228
BsaI GGTCTC 1 cut(s) 1425
BsaJI CCNNGG 7 cut(s) 91, 590, 1675, 2252, 2360, 2842, 2892
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 4 cut(s) 1225, 1255, 1720, 1750
Bsc4I CCNNNNNNNGG 8 cut(s) 888, 889, 1091, 1997, 2258, 2579, 2662, 2810
Bse118I RCCGGY 1 cut(s) 2651
Bse1I ACTGG 4 cut(s) 184, 1325, 1690, 1981
Bse21I CCTNAGG 1 cut(s) 771
Bse3DI GCAATG 1 cut(s) 520
Bse8I GATNNNNATC 3 cut(s) 303, 1479, 2228
BseBI CCWGG 6 cut(s) 93, 1788, 2301, 2412, 2502, 2931
BseDI CCNNGG 7 cut(s) 91, 590, 1675, 2252, 2360, 2842, 2892
BseGI GGATG 4 cut(s) 1215, 1231, 1918, 2377
BseJI GATNNNNATC 3 cut(s) 303, 1479, 2228
BseLI CCNNNNNNNGG 8 cut(s) 888, 889, 1091, 1997, 2258, 2579, 2662, 2810
BseMI GCAATG 1 cut(s) 520
BseMII CTCAG 3 cut(s) 762, 1010, 1514
BseNI ACTGG 4 cut(s) 184, 1325, 1690, 1981
BseRI GAGGAG 1 cut(s) 1051
BseSI GKGCMC 2 cut(s) 2149, 2545
BseXI GCAGC 4 cut(s) 651, 1643, 1784, 2541
BsgI GTGCAG 1 cut(s) 1785
Bsh1236I CGCG 1 cut(s) 2613
BshFI GGCC 7 cut(s) 1577, 1674, 2147, 2277, 2655, 2793, 2847
BsiHKAI GWGCWC 4 cut(s) 354, 769, 2545, 2938
BsiHKCI CYCGRG 1 cut(s) 129
BsiSI CCGG 4 cut(s) 347, 1677, 2159, 2652
BslFI GGGAC 4 cut(s) 563, 696, 729, 2608
BslI CCNNNNNNNGG 8 cut(s) 888, 889, 1091, 1997, 2258, 2579, 2662, 2810
BsmAI GTCTC 2 cut(s) 1425, 2016
BsmFI GGGAC 4 cut(s) 563, 696, 729, 2608
BsmI GAATGC 2 cut(s) 1178, 2113
BsnI GGCC 7 cut(s) 1577, 1674, 2147, 2277, 2655, 2793, 2847
Bso31I GGTCTC 1 cut(s) 1425
BsoBI CYCGRG 1 cut(s) 129
Bsp120I GGGCCC 1 cut(s) 2145
Bsp1286I GDGCHC 6 cut(s) 354, 769, 909, 2149, 2545, 2938
Bsp19I CCATGG 2 cut(s) 590, 2842
BspACI CCGC 5 cut(s) 320, 1336, 1895, 2019, 2613
BspANI GGCC 7 cut(s) 1577, 1674, 2147, 2277, 2655, 2793, 2847
BspCNI CTCAG 3 cut(s) 763, 1009, 1513
BspFNI CGCG 1 cut(s) 2613
BspLI GGNNCC 5 cut(s) 684, 717, 1675, 2147, 2231
BspPI GGATC 5 cut(s) 767, 1024, 2224, 2237, 2564
BspQI GCTCTTC 1 cut(s) 897
BspTNI GGTCTC 1 cut(s) 1425
BsrDI GCAATG 1 cut(s) 520
BsrFI RCCGGY 1 cut(s) 2651
BsrI ACTGG 4 cut(s) 184, 1325, 1690, 1981
BssAI RCCGGY 1 cut(s) 2651
BssECI CCNNGG 7 cut(s) 91, 590, 1675, 2252, 2360, 2842, 2892
BssNAI GTATAC 1 cut(s) 1238
BssT1I CCWWGG 5 cut(s) 590, 2252, 2360, 2842, 2892
Bst1107I GTATAC 1 cut(s) 1238
Bst2UI CCWGG 6 cut(s) 93, 1788, 2301, 2412, 2502, 2931
Bst4CI ACNGT 5 cut(s) 475, 875, 1078, 1165, 1706
Bst6I CTCTTC 2 cut(s) 897, 1399
BstAPI GCANNNNNTGC 1 cut(s) 1715
BstC8I GCNNGC 3 cut(s) 1849, 2083, 2849
BstDEI CTNAG 6 cut(s) 771, 996, 1500, 1724, 1964, 2558
BstDSI CCRYGG 2 cut(s) 590, 2842
BstF5I GGATG 4 cut(s) 1215, 1231, 1918, 2377
BstFNI CGCG 1 cut(s) 2613
BstH2I RGCGCY 1 cut(s) 832
BstHHI GCGC 3 cut(s) 513, 711, 831
BstMAI GTCTC 2 cut(s) 1425, 2016
BstMWI GCNNNNNNNGC 4 cut(s) 773, 1715, 1946, 2209
BstNI CCWGG 6 cut(s) 93, 1788, 2301, 2412, 2502, 2931
BstNSI RCATGY 4 cut(s) 215, 1559, 1851, 2085
BstSCI CCNGG 8 cut(s) 91, 1675, 1786, 2157, 2299, 2410, 2500, 2929
BstSFI CTRYAG 2 cut(s) 871, 2493
BstSLI GKGCMC 2 cut(s) 2149, 2545
BstUI CGCG 1 cut(s) 2613
BstV1I GCAGC 4 cut(s) 651, 1643, 1784, 2541
BstV2I GAAGAC 2 cut(s) 1575, 2827
BstX2I RGATCY 5 cut(s) 524, 759, 1016, 1879, 2229
BstYI RGATCY 5 cut(s) 524, 759, 1016, 1879, 2229
BstZ17I GTATAC 1 cut(s) 1238
Bsu36I CCTNAGG 1 cut(s) 771
BsuI GTATCC 2 cut(s) 1948, 2887
BsuRI GGCC 7 cut(s) 1577, 1674, 2147, 2277, 2655, 2793, 2847
BtgI CCRYGG 2 cut(s) 590, 2842
BtgZI GCGATG 1 cut(s) 184
BtrI CACGTC 3 cut(s) 51, 1892, 2833
BtsCI GGATG 4 cut(s) 1215, 1231, 1918, 2377
BtsIMutI CAGTG 2 cut(s) 480, 1250
Cac8I GCNNGC 3 cut(s) 1849, 2083, 2849
CfoI GCGC 3 cut(s) 513, 711, 831
Cfr10I RCCGGY 1 cut(s) 2651
Cfr13I GGNCC 8 cut(s) 386, 683, 716, 1136, 1673, 2145, 2146, 2896
Csp6I GTAC 5 cut(s) 365, 868, 2048, 2667, 2818
CviQI GTAC 5 cut(s) 365, 868, 2048, 2667, 2818
DdeI CTNAG 6 cut(s) 771, 996, 1500, 1724, 1964, 2558
DraI TTTAAA 2 cut(s) 1816, 2959
DriI GACNNNNNGTC 1 cut(s) 1834
EaeI YGGCCR 3 cut(s) 2275, 2653, 2845
Eam1104I CTCTTC 2 cut(s) 897, 1399
Eam1105I GACNNNNNGTC 1 cut(s) 1834
EarI CTCTTC 2 cut(s) 897, 1399
EciI GGCGGA 2 cut(s) 335, 2034
Ecl136II GAGCTC 2 cut(s) 352, 2936
Eco130I CCWWGG 5 cut(s) 590, 2252, 2360, 2842, 2892
Eco24I GRGCYC 4 cut(s) 354, 909, 2149, 2938
Eco31I GGTCTC 1 cut(s) 1425
Eco47I GGWCC 5 cut(s) 386, 683, 716, 1136, 2896
Eco53kI GAGCTC 2 cut(s) 352, 2936
Eco57I CTGAAG 4 cut(s) 570, 1110, 2688, 2821
Eco81I CCTNAGG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 129
EcoICRI GAGCTC 2 cut(s) 352, 2936
EcoO109I RGGNCCY 3 cut(s) 683, 2145, 2896
EcoRI GAATTC 6 cut(s) 913, 949, 989, 2401, 2480, 2688
EcoRII CCWGG 6 cut(s) 91, 1786, 2299, 2410, 2500, 2929
EcoT14I CCWWGG 5 cut(s) 590, 2252, 2360, 2842, 2892
EcoT22I ATGCAT 5 cut(s) 1615, 2083, 2087, 2242, 2320
EcoT38I GRGCYC 4 cut(s) 354, 909, 2149, 2938
ErhI CCWWGG 5 cut(s) 590, 2252, 2360, 2842, 2892
FaqI GGGAC 4 cut(s) 563, 696, 729, 2608
FauNDI CATATG 1 cut(s) 375
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 1 cut(s) 1237
Fnu4HI GCNGC 4 cut(s) 640, 1632, 1773, 2555
FokI GGATG 4 cut(s) 1222, 1238, 1925, 2384
FriOI GRGCYC 4 cut(s) 354, 909, 2149, 2938
Fsp4HI GCNGC 4 cut(s) 640, 1632, 1773, 2555
FspBI CTAG 1 cut(s) 2361
GlaI GCGC 3 cut(s) 512, 710, 830
GluI GCNGC 4 cut(s) 640, 1632, 1773, 2555
GsuI CTGGAG 3 cut(s) 201, 2484, 2952
HaeII RGCGCY 1 cut(s) 832
HaeIII GGCC 7 cut(s) 1577, 1674, 2147, 2277, 2655, 2793, 2847
HapII CCGG 4 cut(s) 347, 1677, 2159, 2652
HhaI GCGC 3 cut(s) 513, 711, 831
Hin6I GCGC 3 cut(s) 511, 709, 829
HinP1I GCGC 3 cut(s) 511, 709, 829
HincII GTYRAC 1 cut(s) 2526
HindII GTYRAC 1 cut(s) 2526
HindIII AAGCTT 4 cut(s) 785, 1938, 2717, 2761
HinfI GANTC 8 cut(s) 1332, 1649, 1927, 2011, 2267, 2527, 2616, 2644
HpaII CCGG 4 cut(s) 347, 1677, 2159, 2652
HphI GGTGA 5 cut(s) 1106, 2201, 2273, 2426, 2641
Hpy166II GTNNAC 9 cut(s) 26, 1217, 1238, 1322, 1839, 2377, 2526, 2543, 2818
Hpy188I TCNGA 8 cut(s) 15, 335, 466, 1090, 1161, 1870, 2117, 2707
Hpy188III TCNNGA 5 cut(s) 266, 770, 1764, 1809, 2905
Hpy8I GTNNAC 9 cut(s) 26, 1217, 1238, 1322, 1839, 2377, 2526, 2543, 2818
Hpy99I CGWCG 3 cut(s) 1765, 1893, 2742
HpyAV CCTTC 5 cut(s) 828, 1085, 1788, 2712, 2915
HpyCH4III ACNGT 5 cut(s) 475, 875, 1078, 1165, 1706
HpyF10VI GCNNNNNNNGC 4 cut(s) 773, 1715, 1946, 2209
HpyF3I CTNAG 6 cut(s) 771, 996, 1500, 1724, 1964, 2558
HspAI GCGC 3 cut(s) 511, 709, 829
Ksp22I TGATCA 1 cut(s) 304
LguI GCTCTTC 1 cut(s) 897
LmnI GCTCC 4 cut(s) 349, 772, 2503, 2933
Lsp1109I GCAGC 4 cut(s) 651, 1643, 1784, 2541
LweI GCATC 7 cut(s) 739, 865, 1263, 1664, 2068, 2227, 2626
MaeI CTAG 1 cut(s) 2361
MaeIII GTNAC 4 cut(s) 102, 248, 491, 1400
MfeI CAATTG 2 cut(s) 1746, 1824
MflI RGATCY 5 cut(s) 524, 759, 1016, 1879, 2229
MhlI GDGCHC 6 cut(s) 354, 769, 909, 2149, 2545, 2938
MlsI TGGCCA 2 cut(s) 2277, 2847
MluNI TGGCCA 2 cut(s) 2277, 2847
MlyI GAGTC 3 cut(s) 1341, 1921, 2521
MmeI TCCRAC 1 cut(s) 1309
Mox20I TGGCCA 2 cut(s) 2277, 2847
Mph1103I ATGCAT 5 cut(s) 1615, 2083, 2087, 2242, 2320
MroXI GAANNNNTTC 1 cut(s) 788
MscI TGGCCA 2 cut(s) 2277, 2847
MslI CAYNNNNRTG 3 cut(s) 210, 1310, 1625
Msp20I TGGCCA 2 cut(s) 2277, 2847
MspI CCGG 4 cut(s) 347, 1677, 2159, 2652
MspR9I CCNGG 8 cut(s) 93, 1677, 1788, 2159, 2301, 2412, 2502, 2931
MunI CAATTG 2 cut(s) 1746, 1824
Mva1269I GAATGC 2 cut(s) 1178, 2113
MvaI CCWGG 6 cut(s) 93, 1788, 2301, 2412, 2502, 2931
MvnI CGCG 1 cut(s) 2613
MwoI GCNNNNNNNGC 4 cut(s) 773, 1715, 1946, 2209
NciI CCSGG 2 cut(s) 1677, 2159
NcoI CCATGG 2 cut(s) 590, 2842
NdeI CATATG 1 cut(s) 375
NlaIV GGNNCC 5 cut(s) 684, 717, 1675, 2147, 2231
NmuCI GTSAC 2 cut(s) 102, 248
NsiI ATGCAT 5 cut(s) 1615, 2083, 2087, 2242, 2320
NspI RCATGY 4 cut(s) 215, 1559, 1851, 2085
PaeI GCATGC 2 cut(s) 1851, 2085
PaeR7I CTCGAG 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 897
PctI GAATGC 2 cut(s) 1178, 2113
PdmI GAANNNNTTC 1 cut(s) 788
PfeI GAWTC 5 cut(s) 1649, 2011, 2267, 2616, 2644
PflMI CCANNNNNTGG 3 cut(s) 1997, 2579, 2810
PkrI GCNGC 4 cut(s) 641, 1633, 1774, 2556
PleI GAGTC 3 cut(s) 1340, 1921, 2521
PpsI GAGTC 3 cut(s) 1340, 1921, 2521
PpuMI RGGWCCY 2 cut(s) 683, 2896
PshBI ATTAAT 1 cut(s) 804
PsiI TTATAA 2 cut(s) 678, 2354
Psp124BI GAGCTC 2 cut(s) 354, 2938
Psp5II RGGWCCY 2 cut(s) 683, 2896
Psp6I CCWGG 6 cut(s) 91, 1786, 2299, 2410, 2500, 2929
PspGI CCWGG 6 cut(s) 91, 1786, 2299, 2410, 2500, 2929
PspN4I GGNNCC 5 cut(s) 684, 717, 1675, 2147, 2231
PspOMI GGGCCC 1 cut(s) 2145
PspPI GGNCC 8 cut(s) 386, 683, 716, 1136, 1673, 2145, 2146, 2896
PspPPI RGGWCCY 2 cut(s) 683, 2896
PspXI VCTCGAGB 1 cut(s) 129
PsuI RGATCY 5 cut(s) 524, 759, 1016, 1879, 2229
RsaI GTAC 5 cut(s) 366, 869, 2049, 2668, 2819
RsaNI GTAC 5 cut(s) 365, 868, 2048, 2667, 2818
RseI CAYNNNNRTG 3 cut(s) 210, 1310, 1625
SacI GAGCTC 2 cut(s) 354, 2938
SapI GCTCTTC 1 cut(s) 897
SatI GCNGC 4 cut(s) 640, 1632, 1773, 2555
Sau96I GGNCC 8 cut(s) 386, 683, 716, 1136, 1673, 2145, 2146, 2896
SchI GAGTC 3 cut(s) 1341, 1921, 2521
ScrFI CCNGG 8 cut(s) 93, 1677, 1788, 2159, 2301, 2412, 2502, 2931
SduI GDGCHC 6 cut(s) 354, 769, 909, 2149, 2545, 2938
SfaNI GCATC 7 cut(s) 739, 865, 1263, 1664, 2068, 2227, 2626
SfcI CTRYAG 2 cut(s) 871, 2493
Sfr274I CTCGAG 1 cut(s) 129
SinI GGWCC 5 cut(s) 386, 683, 716, 1136, 2896
SlaI CTCGAG 1 cut(s) 129
SmiMI CAYNNNNRTG 3 cut(s) 210, 1310, 1625
SmlI CTYRAG 3 cut(s) 129, 1220, 2787
SmoI CTYRAG 3 cut(s) 129, 1220, 2787
SphI GCATGC 2 cut(s) 1851, 2085
SsiI CCGC 5 cut(s) 320, 1336, 1895, 2019, 2613
SspI AATATT 2 cut(s) 361, 2566
SspMI CTAG 1 cut(s) 2361
SstI GAGCTC 2 cut(s) 354, 2938
StyD4I CCNGG 8 cut(s) 91, 1675, 1786, 2157, 2299, 2410, 2500, 2929
StyI CCWWGG 5 cut(s) 590, 2252, 2360, 2842, 2892
TaaI ACNGT 5 cut(s) 475, 875, 1078, 1165, 1706
TaqI TCGA 4 cut(s) 130, 737, 1260, 1983
TaqII GACCGA 1 cut(s) 126
TatI WGTACW 1 cut(s) 364
TfiI GAWTC 5 cut(s) 1649, 2011, 2267, 2616, 2644
TscAI CASTG 2 cut(s) 480, 1250
TseFI GTSAC 2 cut(s) 102, 248
TseI GCWGC 4 cut(s) 639, 1631, 1772, 2554
Tsp45I GTSAC 2 cut(s) 102, 248
TspGWI ACGGA 3 cut(s) 1303, 1540, 2712
TspRI CASTG 2 cut(s) 480, 1250
Van91I CCANNNNNTGG 3 cut(s) 1997, 2579, 2810
VneI GTGCAC 1 cut(s) 2541
VpaK11BI GGWCC 5 cut(s) 386, 683, 716, 1136, 2896
VspI ATTAAT 1 cut(s) 804
XceI RCATGY 4 cut(s) 215, 1559, 1851, 2085
XcmI CCANNNNNNNNNTGG 1 cut(s) 588
XhoI CTCGAG 1 cut(s) 129
XmaJI CCTAGG 1 cut(s) 2360
XmiI GTMKAC 1 cut(s) 1237
XmnI GAANNNNTTC 1 cut(s) 788
XspI CTAG 1 cut(s) 2361
Zsp2I ATGCAT 5 cut(s) 1615, 2083, 2087, 2242, 2320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.