Rw2G008590

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000080
Physical Location & Seq
Forward (+)
8646364 .. 8651393
5030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G008590.1

Sequence Viewer

Length: 3876 bp
ATGATCCAGCTCTTTTCTGACCAAACAGATGTGGGTAGAAAATTCAGACAAAATATCCGAGCCTATAATAATGTGTTTGCGTTCACTTCAATGGGTGTGCATGTTGATGAATCCATTAATCTTCAAGGTCGTGGAATTTATACTTTTCGTGCACAAGGTTCTATATATCATAAGATTGGTGGGCTTTTACCAAGTGAAGGGGCGAGACCACGATATCTACAAGCTTACATATATGACACCGAGCATGAACTTGACAATCGATTGTCTGAAAGTGTAGTTTTAGACAGGGCACTGGTTGAAAAGATACAACAGATATTGAATCACTATAATCCATTTGTCCATCAATTTCGAAGCCTCGGACAGCGCCAAGACTTGCCCAATTGCAGGCTCATCATAAGAGAGCAGCCTTCTAATCAACGTCAGTATTGTTTACCATCTGCATCACAAGTTGCTGCTATTATAGTAGGAGGGGGCGACATCAATCCGAATGGAAGAGATTTAATTGTCCAAACAATTAGTGGAAGACTCTGGAATGTTAAAGACTCGGTCGGATATTACGACCCCATGCAGTATCCTTTGTTATTACCTTATGGAACATATGGGTGGGATATTAATAGTCATGATGAGAATGGAAGACCTATGTCATGTTGCGACTATTATGCATATATGTTACAGATGCGCCCAGGTGATGAAAATCCTCTACTTCGAGGCGGACGGCTCTTACAACAGTATGTTGTAGATAATTACGTGAAAATTGAATCCCAAAAACTCAGATGGCTTCGCAGTAACCAAGATACAATTAGGAAAGAATTTTATCAAGGACTTCAAGACTCTTTTATTGCCGGGGAAAACAATGCAGGTAACGTCGGTCATAGGACAATTCTGCCATCATCATTTGTTGGAAGTCCACGTGATATGTACCAACGGTACCAAGATGCGATGGCTTTGGTTCAAAAGTATGGCAGACCAGATTTGTTTATCACTATGACGTGTAACCCGAATTGGGAAGAAATTAAAAGTGAGCTACTCCCTGGACAAACATCACAAGATCGTCCAGATTTAGAGGTCAGGGTATTTCGTTCAAAACTTGAGCAGCTAAAAGAAGACATCATCACCAAAGGGGTATTGGGAAGAGTTATTGCTCATGCTTTTGTCGTTGAATTTCAGAAACGGGGCCTACCACATGTACACATGCTGGTCATGTTGGATGAAAATGATAAGCTTAATAACCCTGAAGATTATGATCGCATTGTTAGAGCAGAAATACCAGATAAAGATGAAGAGCCTCAACTTTATGATCTGGTGTTGAAACATATGATTCACGGTCCATGTGGAACCTTAAACCAGCAGTCTTCATGTATGAAGAATGGGAGATGCAATAAAAGTTATCCAAAATCATTTGCAAATTTCACTGTGCAAGGAAACGATGCATATCCTATATATCGAAGACGAGCAAGTCGCTTACCAGTCCCTCTACGTAGATCTGGAGACACAATGGTGGATAATAGTTGGGTTGTTCCTTATAACCCGTGGTTGTTGCTACGGTATGATTGTCACATCAATGTTGAAATATGTGGGAGCATAAAGAGTGTCAAATACTTATATAAGTATGTCTACAAAGGTCCTGATAGAGTTGCAGTTCAAGTGCAATCTGACCCGGAGTTCGATGAAATAAGACAATTTGTTGATGCAAGGTGGGTTTGTGCACCAGAGGCTTTTTGGAGGATATTTAAATTTGTAATGAACAGAATATATCCAACAGTCGACAGATTACAAATACATCTTCCAAACATGCAACAAATCAGATTCGATGCAAATGAGACTGTAGAAGACATTCTAGCAGATGAGCATGCACAGAAGTCTATGCTTACTGAATATTTCACCATGAACCGTATGGATCCGGAAGCAAGACGATATCTGTATCGAGAATTCCCTCAATACTATAGGTGGGATAGTAAGCAAAAAGCTTGGGTGAAAAGAAGGAACAACTACAAGGTAATTGGAAGAATATACAGTGTTTCACCCTCTGAAGGTGAGAAATTTTACCTACGTGTCCTTCTCAATCATATAAGAGGACCGAGATCATTTCGTGATTTGCTTACTATTAATGGAGTTCTGCAAACTACTTTTAAGGAAGCGGCAGAGAAACGAGGTTTGCTGGAAGATGACAATAGCATCCGACAATGTCTGTTAGAGGCCTCTGCAATTAGAATGCCATCAGCTTTGAGAAGATTATTCGTCACAATTTTGGTGTATAGTCAACCAACTGGTGTTCGAAGATTGTGGGATGAATTTTATCCTTTCATGATTGAAGATTATTCTTCTTCAAGTGATATTGTCGATATTTCTGTCAGAAACAGGCTACTTCGAGATCTAAATAGAATGCTAGAACAATTTAATAAGGGCACTGGAGAATTTGATCTGCCTGAAATGACAAGTGAGCCAGAAGATAGTCTAGGAATACCGAGATGTATTGAAGACGAAATTTCTATAAATATCCCGCAAGAAGATATTGATGCAATTGACCACCTTAACAACGACCAAATGATTGCATTCAACACCATAATGGCCGCAGTTGAGGGAAGAGAAAGTGCATTATTTTTTATCGATGGTCCTGGAGGTACTGGGAAGACGTACTTATATCGTGCATTGCTAGCAAAGTTGAGAAGAATGAATCATATAGTCTTAGCCACAACATCATCTGGAATTGCTGCAAATTTATTGTCCGGCGGGAGGACAGCTCACTCTAGATTCAAGATTCCACTCAATGTAGATGCATCATCCATGTGTTCCATAAGTAAACAGTCTGCTCTAGCACGACTGATACAAGATTCCACTGCAATTATTTGGGATGAGGCACCTATGACACATCGACATGTGTTTGAAGCACTTGATCGAACATTTCGAGACATAATGGATGTCGAATTACCGTTTGGTGGAAAGATAATGATTTTTGGGGGAGATTTTCGTCAAGTTCTCCCTGTTATTCCTAAAGGAACGAAGTCTGAACTCATTCAAGCCTCCATTGTGAAAGCAACATTTTGGGCACAAACAAAGATATTGAGATTGAGACAGAATATGAGATCCATAAATGACCACCAATTTGCAGAATTTCTACTTCGTGTTGGCGATGGGAGTGAACAAGTCATTGACGACGAAATGATGAGATTACCAGAACGCATGGTGGTTCCATGGGAAAGTGACCAATCTATCAATCAAATTATTGATGAAGTTTTCCCCAATTTGGGTGATCATGTAAATGATGCAAGATACATGGTGGACAGAGCATTGATTACTCCTATAAACGATGATGTGGACGTGTTGAATGAAAAAATAATCAATATGTTTCCGGGTGAAGAAATTACATTGTACTCATTTGACTCTGTTGAAGATGACATGAGAAATTTGTACCAACCAGAGTTTCTGAACTCAATAACCGCAGGTGGGCTGCCACCCCACAAGTTAACTTTGAAAGTAGGTGCTCCAATCATGCTTTTAAGGAATATTGACCCTAAATTGGGATTGTGTAACGGTACTAGATTGTTGTGTCGTGGTTCGTACCTAAATCTGATTGATGCTGAGATACTAACAGGACAGTTTGCTGGGACTAGAGTTTTTCTACCTAGAATTCCTTTGAAAAGTGCAGAAAATGCTGGGCTACCATTTCAACTAACAAGAAAGCAATTTCCAGTCAAACTGAGCTTCTCTTTGACCATAAACAAATCTCAAGGTCAGACTATCCCACATGTAGGTGTTTACCTCCCAGATCATGTTTTTAGTCATGGACAATTATATGTTGCATTGTCGAGAGGAGTATCAGATTTTGAACCCATTAAAGAAGAATCAGGATCTTGTTGGATTCCTTATTTCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1291

Amino Acids

148.37

Weight (kDa)

6.42

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 219 - 400 4.7e-63 Helitron helicase-like domain at N-terminus
PIF1 PF05970 844 - 1048 1.9e-73 PIF1-like helicase
Pif1_2B_dom PF21530 1140 - 1182 9.4e-21 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1524
AarI CACCTGC 1 cut(s) 3431
AasI GACNNNNNNGTC 1 cut(s) 1455
Acc36I ACCTGC 2 cut(s) 848, 3431
Acc65I GGTACC 1 cut(s) 927
AccB1I GGYRCC 2 cut(s) 927, 2857
AccI GTMKAC 2 cut(s) 1614, 1764
AccIII TCCGGA 1 cut(s) 1900
AciI CCGC 6 cut(s) 711, 2138, 2501, 2571, 2730, 3438
AclWI GGATC 4 cut(s) 1892, 1905, 3078, 3856
AcoI YGGCCR 1 cut(s) 2568
AcuI CTGAAG 2 cut(s) 1254, 2049
AcvI CACGTG 1 cut(s) 911
AfaI GTAC 9 cut(s) 920, 929, 1188, 2623, 2636, 3371, 3410, 3535, 3560
AflIII ACRYGT 6 cut(s) 989, 1183, 2050, 2875, 3318, 3745
AjiI CACGTC 2 cut(s) 990, 3319
AjnI CCWGG 3 cut(s) 682, 1030, 2614
AjuI GAANNNNNNNTTGG 4 cut(s) 2916, 2948, 3405, 3437
AleI CACNNNNGTG 1 cut(s) 1496
AluBI AGCT 9 cut(s) 10, 224, 1024, 1096, 1222, 1967, 2222, 2741, 3702
AluI AGCT 9 cut(s) 10, 224, 1024, 1096, 1222, 1967, 2222, 2741, 3702
Alw21I GWGCWC 3 cut(s) 154, 1708, 3484
Alw26I GTCTC 5 cut(s) 199, 1482, 1814, 2901, 3064
Alw44I GTGCAC 2 cut(s) 150, 1704
AlwI GGATC 4 cut(s) 1892, 1905, 3078, 3856
Aor13HI TCCGGA 1 cut(s) 1900
AoxI GGCC 3 cut(s) 1174, 2196, 2568
ApaLI GTGCAC 2 cut(s) 150, 1704
ApeKI GCWGC 5 cut(s) 403, 452, 1093, 2711, 3448
ArsI GACNNNNNNTTYG 6 cut(s) 1646, 1678, 2708, 2740, 3086, 3118
AseI ATTAAT 3 cut(s) 117, 612, 2106
Asp700I GAANNNNTTC 2 cut(s) 1833, 3012
Asp718I GGTACC 1 cut(s) 927
AspLEI GCGC 2 cut(s) 366, 681
AspS9I GGNCC 5 cut(s) 1174, 1325, 1622, 2075, 2612
AsuC2I CCSGG 3 cut(s) 844, 1658, 3351
AsuHPI GGTGA 8 cut(s) 698, 1105, 1873, 1984, 2013, 2045, 3260, 3365
AsuII TTCGAA 2 cut(s) 349, 2275
AsuNHI GCTAGC 1 cut(s) 2653
AvaII GGWCC 4 cut(s) 1325, 1622, 2075, 2612
BaeGI GKGCMC 5 cut(s) 154, 292, 1708, 2408, 3049
BamHI GGATCC 1 cut(s) 1897
BanI GGYRCC 2 cut(s) 927, 2857
BbrPI CACGTG 1 cut(s) 911
BbsI GAAGAC 8 cut(s) 529, 640, 1110, 1344, 1453, 1836, 2484, 2636
Bbv12I GWGCWC 3 cut(s) 154, 1708, 3484
BbvI GCAGC 5 cut(s) 415, 439, 1105, 2698, 3435
BccI CCATC 8 cut(s) 348, 442, 768, 895, 934, 2224, 2603, 3125
BceAI ACGGC 1 cut(s) 731
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 3 cut(s) 684, 1032, 2616
BciVI GTATCC 1 cut(s) 582
BclI TGATCA 1 cut(s) 3250
BcnI CCSGG 3 cut(s) 844, 1658, 3351
BcoDI GTCTC 5 cut(s) 199, 1482, 1814, 2901, 3064
BfaI CTAG 9 cut(s) 1838, 2387, 2456, 2654, 2748, 2813, 3537, 3609, 3624
BfmI CTRYAG 3 cut(s) 1824, 1942, 3872
BfoI RGCGCY 1 cut(s) 367
BfuAI ACCTGC 2 cut(s) 848, 3431
BfuI GTATCC 1 cut(s) 582
BglII AGATCT 2 cut(s) 1481, 2371
BisI GCNGC 7 cut(s) 404, 453, 1094, 2139, 2571, 2712, 3449
BlsI GCNGC 7 cut(s) 405, 454, 1095, 2140, 2572, 2713, 3450
Bme1390I CCNGG 6 cut(s) 684, 844, 1032, 1658, 2616, 3351
Bme18I GGWCC 4 cut(s) 1325, 1622, 2075, 2612
BmgBI CACGTC 2 cut(s) 990, 3319
BmgT120I GGNCC 5 cut(s) 1174, 1325, 1622, 2075, 2612
BmiI GGNNCC 6 cut(s) 929, 1175, 1336, 1899, 2859, 3189
BmrFI CCNGG 6 cut(s) 684, 844, 1032, 1658, 2616, 3351
BmrI ACTGGG 1 cut(s) 2634
BmtI GCTAGC 1 cut(s) 2657
BmuI ACTGGG 1 cut(s) 2634
BoxI GACNNNNGTC 1 cut(s) 640
BpiI GAAGAC 8 cut(s) 529, 640, 1110, 1344, 1453, 1836, 2484, 2636
BplI GAGNNNNNCTC 2 cut(s) 2725, 2757
BpmI CTGGAG 3 cut(s) 1506, 2430, 2637
Bpu14I TTCGAA 2 cut(s) 349, 2275
BpuEI CTTGAG 2 cut(s) 1109, 3711
BpuMI CCSGG 3 cut(s) 844, 1658, 3351
Bsa29I ATCGAT 2 cut(s) 259, 2607
BsaAI YACGTR 4 cut(s) 748, 911, 1478, 2051
BsaBI GATNNNNATC 3 cut(s) 693, 1242, 1431
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 6 cut(s) 355, 682, 843, 1030, 1529, 3191
BsaWI WCCGGW 1 cut(s) 1900
Bse1I ACTGG 6 cut(s) 297, 1466, 2272, 2413, 2629, 3689
Bse3DI GCAATG 1 cut(s) 2648
Bse8I GATNNNNATC 3 cut(s) 693, 1242, 1431
BseAI TCCGGA 1 cut(s) 1900
BseBI CCWGG 3 cut(s) 684, 1032, 2616
BseCI ATCGAT 2 cut(s) 259, 2607
BseDI CCNNGG 6 cut(s) 355, 682, 843, 1030, 1529, 3191
BseGI GGATG 6 cut(s) 1213, 2175, 2293, 2780, 2857, 2923
BseJI GATNNNNATC 3 cut(s) 693, 1242, 1431
BseMI GCAATG 1 cut(s) 2648
BseMII CTCAG 3 cut(s) 784, 3570, 3689
BseNI ACTGG 6 cut(s) 297, 1466, 2272, 2413, 2629, 3689
BseRI GAGGAG 1 cut(s) 3825
BseSI GKGCMC 5 cut(s) 154, 292, 1708, 2408, 3049
BseXI GCAGC 5 cut(s) 415, 439, 1105, 2698, 3435
BseYI CCCAGC 2 cut(s) 3602, 3653
BsgI GTGCAG 1 cut(s) 3663
Bsh1285I CGRYCG 1 cut(s) 549
BshFI GGCC 3 cut(s) 1176, 2198, 2570
BshNI GGYRCC 2 cut(s) 927, 2857
BshVI ATCGAT 2 cut(s) 259, 2607
BsiEI CGRYCG 1 cut(s) 549
BsiHKAI GWGCWC 3 cut(s) 154, 1708, 3484
BsiSI CCGG 5 cut(s) 843, 1658, 1901, 2727, 3350
BslFI GGGAC 2 cut(s) 1454, 3619
BsmAI GTCTC 5 cut(s) 199, 1482, 1814, 2901, 3064
BsmFI GGGAC 2 cut(s) 1454, 3619
BsmI GAATGC 3 cut(s) 2217, 2388, 2552
BsnI GGCC 3 cut(s) 1176, 2198, 2570
Bso31I GGTCTC 1 cut(s) 199
Bsp119I TTCGAA 2 cut(s) 349, 2275
Bsp1286I GDGCHC 6 cut(s) 154, 292, 1708, 2408, 3049, 3484
Bsp13I TCCGGA 1 cut(s) 1900
Bsp1407I TGTACA 1 cut(s) 1186
Bsp19I CCATGG 1 cut(s) 3191
BspACI CCGC 6 cut(s) 711, 2138, 2501, 2571, 2730, 3438
BspANI GGCC 3 cut(s) 1176, 2198, 2570
BspCNI CTCAG 3 cut(s) 783, 3571, 3690
BspDI ATCGAT 2 cut(s) 259, 2607
BspEI TCCGGA 1 cut(s) 1900
BspHI TCATGA 2 cut(s) 619, 2304
BspLI GGNNCC 6 cut(s) 929, 1175, 1336, 1899, 2859, 3189
BspMI ACCTGC 2 cut(s) 848, 3431
BspOI GCTAGC 1 cut(s) 2657
BspPI GGATC 4 cut(s) 1892, 1905, 3078, 3856
BspQI GCTCTTC 1 cut(s) 1275
BspT104I TTCGAA 2 cut(s) 349, 2275
BspT107I GGYRCC 2 cut(s) 927, 2857
BspTNI GGTCTC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 2648
BsrGI TGTACA 1 cut(s) 1186
BsrI ACTGG 6 cut(s) 297, 1466, 2272, 2413, 2629, 3689
BssECI CCNNGG 6 cut(s) 355, 682, 843, 1030, 1529, 3191
BssT1I CCWWGG 1 cut(s) 3191
Bst2UI CCWGG 3 cut(s) 684, 1032, 2616
Bst6I CTCTTC 4 cut(s) 487, 1126, 1275, 2578
BstAPI GCANNNNNTGC 1 cut(s) 3650
BstAUI TGTACA 1 cut(s) 1186
BstBAI YACGTR 4 cut(s) 748, 911, 1478, 2051
BstBI TTCGAA 2 cut(s) 349, 2275
BstC8I GCNNGC 3 cut(s) 386, 1851, 2655
BstDEI CTNAG 4 cut(s) 770, 2686, 3579, 3698
BstDSI CCRYGG 2 cut(s) 1529, 3191
BstF5I GGATG 6 cut(s) 1213, 2175, 2293, 2780, 2857, 2923
BstH2I RGCGCY 1 cut(s) 367
BstHHI GCGC 2 cut(s) 366, 681
BstMAI GTCTC 5 cut(s) 199, 1482, 1814, 2901, 3064
BstMCI CGRYCG 1 cut(s) 549
BstMWI GCNNNNNNNGC 3 cut(s) 1712, 2654, 3650
BstNI CCWGG 3 cut(s) 684, 1032, 2616
BstNSI RCATGY 7 cut(s) 104, 1187, 1195, 1795, 1853, 2879, 3749
BstPAI GACNNNNGTC 1 cut(s) 640
BstSCI CCNGG 6 cut(s) 682, 842, 1030, 1656, 2614, 3349
BstSFI CTRYAG 3 cut(s) 1824, 1942, 3872
BstSLI GKGCMC 5 cut(s) 154, 292, 1708, 2408, 3049
BstSNI TACGTA 1 cut(s) 1478
BstV1I GCAGC 5 cut(s) 415, 439, 1105, 2698, 3435
BstV2I GAAGAC 8 cut(s) 529, 640, 1110, 1344, 1453, 1836, 2484, 2636
BstX2I RGATCY 5 cut(s) 1481, 1897, 2371, 3083, 3848
BstYI RGATCY 5 cut(s) 1481, 1897, 2371, 3083, 3848
Bsu15I ATCGAT 2 cut(s) 259, 2607
BsuI GTATCC 1 cut(s) 582
BsuRI GGCC 3 cut(s) 1176, 2198, 2570
BsuTUI ATCGAT 2 cut(s) 259, 2607
BtgI CCRYGG 2 cut(s) 1529, 3191
BtgZI GCGATG 2 cut(s) 953, 3144
BtrI CACGTC 2 cut(s) 990, 3319
BtsCI GGATG 6 cut(s) 1213, 2175, 2293, 2780, 2857, 2923
BtsI GCAGTG 1 cut(s) 2835
BtsIMutI CAGTG 5 cut(s) 290, 1410, 2020, 2406, 2835
BveI ACCTGC 2 cut(s) 848, 3431
Cac8I GCNNGC 3 cut(s) 386, 1851, 2655
CciI TCATGA 2 cut(s) 619, 2304
CfoI GCGC 2 cut(s) 366, 681
Cfr13I GGNCC 5 cut(s) 1174, 1325, 1622, 2075, 2612
ClaI ATCGAT 2 cut(s) 259, 2607
Csp6I GTAC 9 cut(s) 919, 928, 1187, 2622, 2635, 3370, 3409, 3534, 3559
CviQI GTAC 9 cut(s) 919, 928, 1187, 2622, 2635, 3370, 3409, 3534, 3559
DdeI CTNAG 4 cut(s) 770, 2686, 3579, 3698
DraI TTTAAA 1 cut(s) 1732
DrdI GACNNNNNNGTC 1 cut(s) 1455
DseDI GACNNNNNNGTC 1 cut(s) 1455
EaeI YGGCCR 1 cut(s) 2568
Eam1104I CTCTTC 4 cut(s) 487, 1126, 1275, 2578
EarI CTCTTC 4 cut(s) 487, 1126, 1275, 2578
EciI GGCGGA 1 cut(s) 726
Eco105I TACGTA 1 cut(s) 1478
Eco130I CCWWGG 1 cut(s) 3191
Eco147I AGGCCT 1 cut(s) 2198
Eco31I GGTCTC 1 cut(s) 199
Eco32I GATATC 2 cut(s) 215, 1916
Eco47I GGWCC 4 cut(s) 1325, 1622, 2075, 2612
Eco57I CTGAAG 2 cut(s) 1254, 2049
Eco72I CACGTG 1 cut(s) 911
EcoO109I RGGNCCY 2 cut(s) 1174, 1622
EcoRI GAATTC 2 cut(s) 1928, 3627
EcoRII CCWGG 3 cut(s) 682, 1030, 2614
EcoRV GATATC 2 cut(s) 215, 1916
EcoT14I CCWWGG 1 cut(s) 3191
EcoT22I ATGCAT 3 cut(s) 664, 1432, 2779
ErhI CCWWGG 1 cut(s) 3191
FalI AAGNNNNNCTT 2 cut(s) 2621, 2653
FaqI GGGAC 2 cut(s) 1454, 3619
FauI CCCGC 2 cut(s) 2508, 2723
FauNDI CATATG 2 cut(s) 598, 1314
FbaI TGATCA 1 cut(s) 3250
FblI GTMKAC 2 cut(s) 1614, 1764
Fnu4HI GCNGC 7 cut(s) 404, 453, 1094, 2139, 2571, 2712, 3449
FokI GGATG 6 cut(s) 1220, 2162, 2300, 2767, 2864, 2930
Fsp4HI GCNGC 7 cut(s) 404, 453, 1094, 2139, 2571, 2712, 3449
FspBI CTAG 9 cut(s) 1838, 2387, 2456, 2654, 2748, 2813, 3537, 3609, 3624
GlaI GCGC 2 cut(s) 365, 680
GluI GCNGC 7 cut(s) 404, 453, 1094, 2139, 2571, 2712, 3449
GsaI CCCAGC 2 cut(s) 3606, 3657
GsuI CTGGAG 3 cut(s) 1506, 2430, 2637
HaeII RGCGCY 1 cut(s) 367
HaeIII GGCC 3 cut(s) 1176, 2198, 2570
HapII CCGG 5 cut(s) 843, 1658, 1901, 2727, 3350
HhaI GCGC 2 cut(s) 366, 681
Hin6I GCGC 2 cut(s) 364, 679
HinP1I GCGC 2 cut(s) 364, 679
HincII GTYRAC 3 cut(s) 1765, 2261, 3465
HindII GTYRAC 3 cut(s) 1765, 2261, 3465
HindIII AAGCTT 3 cut(s) 222, 1220, 1965
HpaI GTTAAC 1 cut(s) 3465
HpaII CCGG 5 cut(s) 843, 1658, 1901, 2727, 3350
HphI GGTGA 8 cut(s) 698, 1105, 1873, 1984, 2013, 2045, 3260, 3365
Hpy99I CGWCG 2 cut(s) 869, 3158
HpyAV CCTTC 5 cut(s) 191, 417, 1974, 2024, 2066
HpyCH4IV ACGT 9 cut(s) 418, 747, 864, 910, 989, 1477, 2050, 2633, 3318
HpyF10VI GCNNNNNNNGC 3 cut(s) 1712, 2654, 3650
HpyF3I CTNAG 4 cut(s) 770, 2686, 3579, 3698
HpySE526I ACGT 9 cut(s) 418, 747, 864, 910, 989, 1477, 2050, 2633, 3318
HspAI GCGC 2 cut(s) 364, 679
Kpn2I TCCGGA 1 cut(s) 1900
KpnI GGTACC 1 cut(s) 931
Ksp22I TGATCA 1 cut(s) 3250
KspAI GTTAAC 1 cut(s) 3465
LguI GCTCTTC 1 cut(s) 1275
LmnI GCTCC 2 cut(s) 1578, 3487
Lsp1109I GCAGC 5 cut(s) 415, 439, 1105, 2698, 3435
MaeI CTAG 9 cut(s) 1838, 2387, 2456, 2654, 2748, 2813, 3537, 3609, 3624
MaeII ACGT 9 cut(s) 418, 747, 864, 910, 989, 1477, 2050, 2633, 3318
MaeIII GTNAC 8 cut(s) 669, 785, 860, 992, 1553, 2239, 3200, 3527
MfeI CAATTG 2 cut(s) 379, 2520
MflI RGATCY 5 cut(s) 1481, 1897, 2371, 3083, 3848
MhlI GDGCHC 6 cut(s) 154, 292, 1708, 2408, 3049, 3484
MlyI GAGTC 4 cut(s) 519, 536, 824, 3374
MmeI TCCRAC 6 cut(s) 529, 880, 1185, 1781, 2203, 3836
Mph1103I ATGCAT 3 cut(s) 664, 1432, 2779
MroI TCCGGA 1 cut(s) 1900
MroXI GAANNNNTTC 2 cut(s) 1833, 3012
MspI CCGG 5 cut(s) 843, 1658, 1901, 2727, 3350
MspR9I CCNGG 6 cut(s) 684, 844, 1032, 1658, 2616, 3351
MunI CAATTG 2 cut(s) 379, 2520
Mva1269I GAATGC 3 cut(s) 2217, 2388, 2552
MvaI CCWGG 3 cut(s) 684, 1032, 2616
MwoI GCNNNNNNNGC 3 cut(s) 1712, 2654, 3650
NciI CCSGG 3 cut(s) 844, 1658, 3351
NcoI CCATGG 1 cut(s) 3191
NdeI CATATG 2 cut(s) 598, 1314
NheI GCTAGC 1 cut(s) 2653
NlaIV GGNNCC 6 cut(s) 929, 1175, 1336, 1899, 2859, 3189
NmuCI GTSAC 3 cut(s) 1553, 2239, 3200
NsiI ATGCAT 3 cut(s) 664, 1432, 2779
NspI RCATGY 7 cut(s) 104, 1187, 1195, 1795, 1853, 2879, 3749
NspV TTCGAA 2 cut(s) 349, 2275
OliI CACNNNNGTG 1 cut(s) 1496
PaeI GCATGC 1 cut(s) 1853
PagI TCATGA 2 cut(s) 619, 2304
PaqCI CACCTGC 1 cut(s) 3431
PceI AGGCCT 1 cut(s) 2198
PciI ACATGT 3 cut(s) 1183, 2875, 3745
PciSI GCTCTTC 1 cut(s) 1275
PcsI WCGNNNNNNNCGW 3 cut(s) 555, 995, 2902
PctI GAATGC 3 cut(s) 2217, 2388, 2552
PdmI GAANNNNTTC 2 cut(s) 1833, 3012
PflFI GACNNNGTC 2 cut(s) 545, 2184
PfoI TCCNGGA 1 cut(s) 2614
PkrI GCNGC 7 cut(s) 405, 454, 1095, 2140, 2572, 2713, 3450
PleI GAGTC 4 cut(s) 519, 536, 824, 3374
PmaCI CACGTG 1 cut(s) 911
PmlI CACGTG 1 cut(s) 911
PpsI GAGTC 4 cut(s) 519, 536, 824, 3374
Ppu21I YACGTR 4 cut(s) 748, 911, 1478, 2051
PpuMI RGGWCCY 1 cut(s) 1622
PscI ACATGT 3 cut(s) 1183, 2875, 3745
PshAI GACNNNNGTC 1 cut(s) 640
PshBI ATTAAT 3 cut(s) 117, 612, 2106
PsiI TTATAA 1 cut(s) 1524
Psp5II RGGWCCY 1 cut(s) 1622
Psp6I CCWGG 3 cut(s) 682, 1030, 2614
PspCI CACGTG 1 cut(s) 911
PspFI CCCAGC 2 cut(s) 3602, 3653
PspGI CCWGG 3 cut(s) 682, 1030, 2614
PspN4I GGNNCC 6 cut(s) 929, 1175, 1336, 1899, 2859, 3189
PspPI GGNCC 5 cut(s) 1174, 1325, 1622, 2075, 2612
PspPPI RGGWCCY 1 cut(s) 1622
PsuI RGATCY 5 cut(s) 1481, 1897, 2371, 3083, 3848
PsyI GACNNNGTC 2 cut(s) 545, 2184
RsaI GTAC 9 cut(s) 920, 929, 1188, 2623, 2636, 3371, 3410, 3535, 3560
RsaNI GTAC 9 cut(s) 919, 928, 1187, 2622, 2635, 3370, 3409, 3534, 3559
SalI GTCGAC 1 cut(s) 1763
SapI GCTCTTC 1 cut(s) 1275
SatI GCNGC 7 cut(s) 404, 453, 1094, 2139, 2571, 2712, 3449
Sau96I GGNCC 5 cut(s) 1174, 1325, 1622, 2075, 2612
SchI GAGTC 4 cut(s) 519, 536, 824, 3374
ScrFI CCNGG 6 cut(s) 684, 844, 1032, 1658, 2616, 3351
SduI GDGCHC 6 cut(s) 154, 292, 1708, 2408, 3049, 3484
SfcI CTRYAG 3 cut(s) 1824, 1942, 3872
SfuI TTCGAA 2 cut(s) 349, 2275
SinI GGWCC 4 cut(s) 1325, 1622, 2075, 2612
SmiI ATTTAAAT 1 cut(s) 1732
SmlI CTYRAG 2 cut(s) 1088, 3726
SmoI CTYRAG 2 cut(s) 1088, 3726
SnaBI TACGTA 1 cut(s) 1478
SphI GCATGC 1 cut(s) 1853
SseBI AGGCCT 1 cut(s) 2198
SsiI CCGC 6 cut(s) 711, 2138, 2501, 2571, 2730, 3438
SspI AATATT 2 cut(s) 1877, 3505
SspMI CTAG 9 cut(s) 1838, 2387, 2456, 2654, 2748, 2813, 3537, 3609, 3624
StuI AGGCCT 1 cut(s) 2198
StyD4I CCNGG 6 cut(s) 682, 842, 1030, 1656, 2614, 3349
StyI CCWWGG 1 cut(s) 3191
SwaI ATTTAAAT 1 cut(s) 1732
TaiI ACGT 9 cut(s) 421, 750, 867, 913, 992, 1480, 2053, 2636, 3321
TaqII GACCGA 3 cut(s) 535, 857, 2092
TatI WGTACW 2 cut(s) 1186, 3369
TauI GCSGC 2 cut(s) 2141, 2573
TscAI CASTG 5 cut(s) 297, 1417, 2020, 2413, 2842
TseFI GTSAC 3 cut(s) 1553, 2239, 3200
TseI GCWGC 5 cut(s) 403, 452, 1093, 2711, 3448
Tsp45I GTSAC 3 cut(s) 1553, 2239, 3200
TspRI CASTG 5 cut(s) 297, 1417, 2020, 2413, 2842
Tth111I GACNNNGTC 2 cut(s) 545, 2184
VneI GTGCAC 2 cut(s) 150, 1704
VpaK11BI GGWCC 4 cut(s) 1325, 1622, 2075, 2612
VspI ATTAAT 3 cut(s) 117, 612, 2106
XbaI TCTAGA 1 cut(s) 2747
XceI RCATGY 7 cut(s) 104, 1187, 1195, 1795, 1853, 2879, 3749
XcmI CCANNNNNNNNNTGG 4 cut(s) 515, 1123, 1716, 1891
XmiI GTMKAC 2 cut(s) 1614, 1764
XmnI GAANNNNTTC 2 cut(s) 1833, 3012
XspI CTAG 9 cut(s) 1838, 2387, 2456, 2654, 2748, 2813, 3537, 3609, 3624
Zsp2I ATGCAT 3 cut(s) 664, 1432, 2779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.