Rw6G017850

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000080
Physical Location & Seq
Forward (+)
35298469 .. 35302630
4162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017850.1

Sequence Viewer

Length: 3978 bp
ATGTGTTGCTTGAATGGGAAAATTGTAATTCCTCCAGTTCAATCTCCTCCAGAAATGGCCGAGCTATTTTCTGCAGAAACTCCAAGAGGCGCCCATTTTAGGCAGAATATTCGTGCTTATAATCATGTATTTTCATTCACATCAATGGGCGTGCATGTCGACCAAAATCTAGCGACTGGTGGTGGCACATTCACTTTTCGTGCTCAAGGATCCATATATCATAAGATAGGAACTCTTCTACCAAATGAGGATAACAGACCAAGATTTCTACAACTGTATATATATGATACAGACCATGAAGTACAAAATCGTATGTTAGAGAATGAATCATTGCATAGAGATGTGGTTGAAACTATACAAGAAATCTTGAATCGGCATAATCCATTTGTGCGGACACTTCATCACCTAGCACAACGCGAAGATTTGGAAACTTGCAGACTCATCATTAGAGAGCAACCTACAAACCAACGTCAGTATAGTTTGCCTACAGAGTCTCAGGTTGCTGCAGTAATAGTGGGTGCAGATGATGCAGAAAATTTGAGAGGTCGTGATATTGTAGTACAGACAAGAGAGGGTCAACTTTTAAATGTCCAAGACTGTGCGGGCTACTATGATCCTTTACAATATCCATTGCTTTTACCTTATGGCACATATGGATGGGATGTGAATAGTCGGAATAATAATGGTACAAAATTGACGTGTCGTGACTATTACGCATATATTTTGCAGATTCGTCCAGATGATGAATCAATTTTACTTCGAGGAGGCCGCCTGTTACAACAATATGTCGTCGATAATTATGTGAAAATTGAGACACAAAAGCTTAGATGGATTCGCAGTAATCAAGATACCCTGCGACGTGAACTTTATAATGGACTCCAAGACTCGCTAAATGCCGGTGAAAACAATGCAGGTAACATTGGTCGCAGAACTATTTTGCCTTCCTCATTTATCGGTAGTCCACGTGATATGCACCAGAGATACCAAGATGCAATGGCTTTGGTCCAAAAATACGGGAAGCCAGATATTTTTCTCACAATGACATGCAACCCGAATTGGGAAGAAATAAAGGCAGAGTTATTACCAGGTCAAGTGGCACAAGATCGCCCAGATTTATGTACCCGGATTTTTCGTGCCAAATTTGAAGAGCTGAAGGAAGACGTTATCAAAAAAGGGGTCTTAGGTATTGTTGTTGCACATGTTTATGTCATCGAATTTCAAAAACGTGGTCTCCCACATGTTCATATGCTTCTAATGTTAAATGAAGATGATAAGTTGAATAACCCGGATGATTATGACAAAATTGTTCGAGTTGAAATACCAAGTCAAGATGAAGAACTGGAGTTGTACAATGCCGTATTGAGGCACATGATACATGGTCCATGTGGAGTACATAAGCGTAATGCCCCATGTATGAAGCGTGGGAGTTGTAAACGAGGTTATCCTAAGCCCTTTTCAGCCAACACATTTCAAGGGAATGACTCTTATCCCATTTACCGTAGGCGAGATGTTCACCCAAATGCGGAATCTGATTTCAATCAAAGCAGACAAGTAGATAACAGTTGGGTTATTCCGTACAACCCTTGGCTACTCACAAAATATGATTGTCATATCAATGTCGAAGTCTGTTGCAGCATTAAGAGTGTTAAGTATTTGTACAAATATGTCTACAAAGGTCCAGATAGAGTAACATTTGAAGTTCGACCCGAAGCTAATGAAGATGAAATAAGGAATTTTGTTGATGCAAGATGGGTTTGTGCACCAGAGGCTTTGTGGAGAATATTCAAATTTGTAATGAATCGTATGTATCCTTCAGTGGAACGATTGCAAATTCATCTTCCAGATAGGCAAAGTGTTTTCTTTAATGCCAATGAAAGGGTAACAGATGTTCTCGCAAATGATAGAAACTCAATGACGATGCTCACAGAGTTTTTTACTAAGAACATGTATTATGAAGGTGCGCGTCAATACTTATATCGAGAGTTTCCTGAAAGATTTAGATGGATTGGCAAACATAAGACATGGGAGGAAAGGCAGACTAACCAAAAAGTAATTGGTAGAATATATACGGTATCTCCTTCAGAAGGAGAAAAGTTCTACTTACGTGTTCTTTTGAACCATGTTAGAGGGCCTACATCATACGAGGATTTAAGGACAGTAGATGGCATATTGCAACCAACATTTAAGAAAGCGGCTGAACAACGAGGTCTGTTAGAAGACGATGATAGTATCCGACAATGTTTACATGAGGCTTCAAGCATACATATGCCTTCATCTTTAAGGAGATTGTTTGTCACTATCTTGGTTTATTGCGAGCCTCATGGGGTACGAAGTTTATGGGATGAATTTTACCAATTTATGATAGAAGATTATGCTTCTTCAAGCATTGCAGTCAGTGCACGTATCACAAACAGACTTTTACGAGACTTAAACGGATTGCTGGTGCAACATAGTAAATCTGTTTGTGATTATGATTTGCCTGAAATGGCTGAAGATTATGGTGAAGATTCGTCAATGCCTAGGCTAATACAAGATGAAGTTTCAATTATCATTCCTCAAGAAGACTGCGATGCAGTTCATCACTTGAATGAGGATCAGACATTTGCATACAATGCAATAATATCTGCTATTGAGTGCAATGCCAATGCCATATTTTTCGTTGATGGTCCAGGAGGGACTGGAAAAACATACTTATATCGTGCACTTTTAGCAACCTTGAGAAGCAATGATCAAATTGTACTGGCAACAGCAACTTCTGGAATAGCAGCAACAATAATGCCTGGTGGGAGAACGGCACACTCCAGGTTTAAAATTCCACTCAATCTTGATGCATCTTCTACATGCTCTATTAGTAAACAATCTAATTTAGCAGAGTTGATACGAAGATCATCAGCGATTATTTGTGATGAGGCACCAATGATGCATAGATTTGCATTTGAAGCTCTTGATCGGACTTTTAAAGACATAATAGGTGTTAATTTGCCATTTGGGGGCAAAGTGATTATTTTTGGGGGAGATTTCCGACAAGTTCTGCCCGTTGTCCATAAGGGTACAAGGTCTCAGATGGTCCAAGCCAGTCTGGTTAACTCTTCATTTTGGAAAGATGTGAGGATTCTTCGTTTGCGGCAAAATATGAGATCCTTCAATGATCCAGAGTTCTCAGAGTTTTTACTTAATGTTGGTAATGGAGAACAACCATCTAGGGTTGAAGACATGATACGATTACCGTCGTGTATGGCCATACCATGGGAGGGTGAAGAGTCAATCAACCGATTAGTTAATGAAGTTTTTCCTAATTTGGAATCTCATGTAAATGATGCAGCGTACATGGTTGAAAGGGCAATAATCACTCCAAAGAATGATGATGTTGACGTGCTTAATGAGATGATTATAAAGCAATTTCCAGGGCCTGAACGGATCCTGTACTCTTTCGACTCAGTTGAGGATGACACCAAAAATATGTACCAACAAGAATTCTTAAATTCAATTTCACCTTCTGGTATGCCCCCACATCAGTTAACTATAAAAAAAGGTGCTCCCATTATGCTTTTGAGAAATATTGACCCTACGATGGGATTGTGCAATGGTACACGATTGACTTGCCGTGGATCATATAACAACCTTATTGATGCAGAGATTTTAACTGGTCAATTTGCTGGAACTAGAGTATTCTTACCAAGAATATCTCTAAAGAGTGCCGAGAGTTCTGGACTCCCTTTTGAGATGACAAGAAAACAATTCCCTGTAAAGTTGAGTTTTGCACTTACTATAAATAAATCACAAGGGCAAACGATACCGAATGTTGGCATCTACCTTCCAGATCATGTATTCAGTCATGGGCAGTTATATGTCGCTTTATCACGAGGAATTTCAGAGGCTACCACCAAAGTGTTAGTCAAGAAAGGCTCATTTGAAGGCGAGGAAGGGGTGTTCACAACAAATGTTGTTTATAAAGAGATTTTGCTTCCAGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1325

Amino Acids

151.59

Weight (kDa)

6.23

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 237 - 418 9.5e-65 Helitron helicase-like domain at N-terminus
PIF1 PF05970 862 - 1068 6e-75 PIF1-like helicase
AAA_30 PF13604 862 - 1025 2.7e-06 AAA domain
Pif1_2B_dom PF21530 1158 - 1200 7.1e-20 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 120, 870, 3392, 3951
Acc36I ACCTGC 1 cut(s) 902
AccB1I GGYRCC 2 cut(s) 89, 2911
AccB7I CCANNNNNTGG 1 cut(s) 3246
AccI GTMKAC 2 cut(s) 159, 1668
AccII CGCG 2 cut(s) 417, 1963
AciI CCGC 6 cut(s) 391, 602, 769, 1523, 2192, 3124
AclWI GGATC 9 cut(s) 204, 217, 608, 2601, 3132, 3143, 3412, 3425, 3616
AcoI YGGCCR 2 cut(s) 57, 3237
AcuI CTGAAG 4 cut(s) 1172, 1797, 2064, 2511
AcvI CACGTG 1 cut(s) 965
AcyI GRCGYC 1 cut(s) 90
AflIII ACRYGT 5 cut(s) 698, 1198, 1237, 1944, 2104
AjiI CACGTC 3 cut(s) 699, 860, 3373
AjnI CCWGG 5 cut(s) 1084, 2668, 2779, 2801, 3403
AleI CACNNNNGTG 1 cut(s) 3887
AloI GAACNNNNNNTCC 2 cut(s) 3914, 3946
AluBI AGCT 5 cut(s) 64, 823, 1150, 1712, 2942
AluI AGCT 5 cut(s) 64, 823, 1150, 1712, 2942
Alw21I GWGCWC 5 cut(s) 205, 1762, 2401, 2704, 3538
Alw26I GTCTC 5 cut(s) 498, 806, 1235, 2418, 3063
Alw44I GTGCAC 3 cut(s) 1758, 2397, 2700
AlwI GGATC 9 cut(s) 204, 217, 608, 2601, 3132, 3143, 3412, 3425, 3616
AlwNI CAGNNNCTG 1 cut(s) 3410
AoxI GGCC 5 cut(s) 57, 766, 2129, 3237, 3407
ApaLI GTGCAC 3 cut(s) 1758, 2397, 2700
ApeKI GCWGC 4 cut(s) 503, 1632, 2765, 3320
ArsI GACNNNNNNTTYG 6 cut(s) 1161, 1193, 1213, 1245, 3879, 3911
Asp700I GAANNNNTTC 1 cut(s) 3288
AspA2I CCTAGG 1 cut(s) 2519
AspLEI GCGC 2 cut(s) 92, 1963
AspS9I GGNCC 7 cut(s) 1003, 1379, 1676, 2129, 2666, 3067, 3407
AsuC2I CCSGG 2 cut(s) 1123, 1286
AsuHPI GGTGA 6 cut(s) 395, 911, 1505, 2513, 3266, 3483
AvaII GGWCC 5 cut(s) 1003, 1379, 1676, 2666, 3067
AvrII CCTAGG 1 cut(s) 2519
BaeGI GKGCMC 3 cut(s) 1762, 2401, 2704
BalI TGGCCA 1 cut(s) 3239
BamHI GGATCC 2 cut(s) 209, 3417
BanI GGYRCC 2 cut(s) 89, 2911
BarI GAAGNNNNNNTAC 4 cut(s) 1795, 1827, 3201, 3233
BauI CACGAG 1 cut(s) 3861
BbrPI CACGTG 1 cut(s) 965
BbsI GAAGAC 4 cut(s) 1164, 2223, 2568, 3216
Bbv12I GWGCWC 5 cut(s) 205, 1762, 2401, 2704, 3538
BbvI GCAGC 4 cut(s) 490, 1644, 2777, 3332
BccI CCATC 9 cut(s) 651, 822, 1743, 1995, 2156, 2657, 3058, 3205, 3565
BceAI ACGGC 3 cut(s) 1340, 2808, 3588
BcgI CGANNNNNNTGC 4 cut(s) 92, 126, 3582, 3616
BciT130I CCWGG 5 cut(s) 1086, 2670, 2781, 2803, 3405
BciVI GTATCC 2 cut(s) 1818, 2240
BclI TGATCA 1 cut(s) 2728
BcnI CCSGG 2 cut(s) 1123, 1286
BcoDI GTCTC 5 cut(s) 498, 806, 1235, 2418, 3063
BfaI CTAG 5 cut(s) 170, 407, 2520, 3201, 3663
BfmI CTRYAG 3 cut(s) 72, 486, 504
BfoI RGCGCY 1 cut(s) 93
BfuAI ACCTGC 1 cut(s) 902
BfuI GTATCC 2 cut(s) 1818, 2240
BisI GCNGC 7 cut(s) 504, 769, 1633, 2193, 2766, 3125, 3321
BlnI CCTAGG 1 cut(s) 2519
BlsI GCNGC 7 cut(s) 505, 770, 1634, 2194, 2767, 3126, 3322
Bme1390I CCNGG 7 cut(s) 1086, 1123, 1286, 2670, 2781, 2803, 3405
Bme18I GGWCC 5 cut(s) 1003, 1379, 1676, 2666, 3067
BmgBI CACGTC 3 cut(s) 699, 860, 3373
BmgT120I GGNCC 7 cut(s) 1003, 1379, 1676, 2129, 2666, 3067, 3407
BmiI GGNNCC 4 cut(s) 91, 211, 2913, 3419
BmrFI CCNGG 7 cut(s) 1086, 1123, 1286, 2670, 2781, 2803, 3405
BpiI GAAGAC 4 cut(s) 1164, 2223, 2568, 3216
BpmI CTGGAG 4 cut(s) 18, 33, 1361, 2785
Bpu10I CCTNAGC 1 cut(s) 1446
BpuEI CTTGAG 3 cut(s) 189, 2541, 2737
BpuMI CCSGG 2 cut(s) 1123, 1286
BsaAI YACGTR 3 cut(s) 965, 2105, 2402
BsaBI GATNNNNATC 1 cut(s) 1536
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 2 cut(s) 1235, 3063
BsaJI CCNNGG 5 cut(s) 1583, 2519, 3245, 3404, 3604
BsaXI ACNNNNNCTCC 4 cut(s) 1215, 1245, 2059, 2089
Bse118I RCCGGY 1 cut(s) 896
Bse1I ACTGG 7 cut(s) 35, 181, 1344, 2683, 2745, 3075, 3649
Bse3DI GCAATG 7 cut(s) 329, 629, 999, 2385, 2644, 2731, 3589
Bse8I GATNNNNATC 1 cut(s) 1536
BseBI CCWGG 5 cut(s) 1086, 2670, 2781, 2803, 3405
BseDI CCNNGG 5 cut(s) 1583, 2519, 3245, 3404, 3604
BseGI GGATG 5 cut(s) 662, 667, 1294, 2347, 3451
BseJI GATNNNNATC 1 cut(s) 1536
BseMI GCAATG 7 cut(s) 329, 629, 999, 2385, 2644, 2731, 3589
BseMII CTCAG 4 cut(s) 509, 3074, 3174, 3450
BseNI ACTGG 7 cut(s) 35, 181, 1344, 2683, 2745, 3075, 3649
BseRI GAGGAG 2 cut(s) 36, 777
BseSI GKGCMC 3 cut(s) 1762, 2401, 2704
BseXI GCAGC 4 cut(s) 490, 1644, 2777, 3332
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 2 cut(s) 417, 1963
BshFI GGCC 5 cut(s) 59, 768, 2131, 3239, 3409
BshNI GGYRCC 2 cut(s) 89, 2911
BsiHKAI GWGCWC 5 cut(s) 205, 1762, 2401, 2704, 3538
BsiSI CCGG 3 cut(s) 897, 1123, 1286
BslFI GGGAC 1 cut(s) 2689
BsmAI GTCTC 5 cut(s) 498, 806, 1235, 2418, 3063
BsmFI GGGAC 1 cut(s) 2689
BsnI GGCC 5 cut(s) 59, 768, 2131, 3239, 3409
Bso31I GGTCTC 2 cut(s) 1235, 3063
Bsp1286I GDGCHC 5 cut(s) 205, 1762, 2401, 2704, 3538
Bsp1407I TGTACA 2 cut(s) 1347, 1656
Bsp19I CCATGG 1 cut(s) 3245
BspACI CCGC 6 cut(s) 391, 602, 769, 1523, 2192, 3124
BspANI GGCC 5 cut(s) 59, 768, 2131, 3239, 3409
BspCNI CTCAG 4 cut(s) 508, 3073, 3173, 3449
BspFNI CGCG 2 cut(s) 417, 1963
BspLI GGNNCC 4 cut(s) 91, 211, 2913, 3419
BspMAI CTGCAG 2 cut(s) 76, 508
BspMI ACCTGC 1 cut(s) 902
BspPI GGATC 9 cut(s) 204, 217, 608, 2601, 3132, 3143, 3412, 3425, 3616
BspQI GCTCTTC 1 cut(s) 1140
BspT107I GGYRCC 2 cut(s) 89, 2911
BspTNI GGTCTC 2 cut(s) 1235, 3063
BsrDI GCAATG 7 cut(s) 329, 629, 999, 2385, 2644, 2731, 3589
BsrFI RCCGGY 1 cut(s) 896
BsrGI TGTACA 2 cut(s) 1347, 1656
BsrI ACTGG 7 cut(s) 35, 181, 1344, 2683, 2745, 3075, 3649
BssAI RCCGGY 1 cut(s) 896
BssECI CCNNGG 5 cut(s) 1583, 2519, 3245, 3404, 3604
BssNI GRCGYC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 3861
BssT1I CCWWGG 3 cut(s) 1583, 2519, 3245
Bst2BI CACGAG 1 cut(s) 3861
Bst2UI CCWGG 5 cut(s) 1086, 2670, 2781, 2803, 3405
Bst4CI ACNGT 7 cut(s) 276, 599, 1499, 1562, 2071, 2158, 3228
Bst6I CTCTTC 4 cut(s) 240, 1140, 3094, 3252
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 3 cut(s) 527, 2396, 2612
BstAUI TGTACA 2 cut(s) 1347, 1656
BstBAI YACGTR 3 cut(s) 965, 2105, 2402
BstC8I GCNNGC 3 cut(s) 152, 604, 2315
BstDEI CTNAG 8 cut(s) 495, 824, 1180, 1446, 1938, 3060, 3160, 3436
BstDSI CCRYGG 2 cut(s) 3245, 3604
BstENI CCTNNNNNAGG 1 cut(s) 2082
BstF5I GGATG 5 cut(s) 662, 667, 1294, 2347, 3451
BstFNI CGCG 2 cut(s) 417, 1963
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 1963
BstMAI GTCTC 5 cut(s) 498, 806, 1235, 2418, 3063
BstMWI GCNNNNNNNGC 6 cut(s) 527, 1766, 2396, 2612, 2708, 2939
BstNI CCWGG 5 cut(s) 1086, 2670, 2781, 2803, 3405
BstNSI RCATGY 6 cut(s) 158, 1047, 1202, 1241, 1948, 2844
BstSCI CCNGG 7 cut(s) 1084, 1121, 1284, 2668, 2779, 2801, 3403
BstSFI CTRYAG 3 cut(s) 72, 486, 504
BstSLI GKGCMC 3 cut(s) 1762, 2401, 2704
BstUI CGCG 2 cut(s) 417, 1963
BstV1I GCAGC 4 cut(s) 490, 1644, 2777, 3332
BstV2I GAAGAC 4 cut(s) 1164, 2223, 2568, 3216
BstX2I RGATCY 3 cut(s) 209, 3137, 3417
BstYI RGATCY 3 cut(s) 209, 3137, 3417
BsuI GTATCC 2 cut(s) 1818, 2240
BsuRI GGCC 5 cut(s) 59, 768, 2131, 3239, 3409
BtgI CCRYGG 2 cut(s) 3245, 3604
BtgZI GCGATG 1 cut(s) 2583
BtrI CACGTC 3 cut(s) 699, 860, 3373
BtsCI GGATG 5 cut(s) 662, 667, 1294, 2347, 3451
BtsIMutI CAGTG 2 cut(s) 1821, 2401
BveI ACCTGC 1 cut(s) 902
Cac8I GCNNGC 3 cut(s) 152, 604, 2315
CaiI CAGNNNCTG 1 cut(s) 3410
CfoI GCGC 2 cut(s) 92, 1963
Cfr10I RCCGGY 1 cut(s) 896
Cfr13I GGNCC 7 cut(s) 1003, 1379, 1676, 2129, 2666, 3067, 3407
CseI GACGC 1 cut(s) 1952
CsiI ACCWGGT 1 cut(s) 1084
DdeI CTNAG 8 cut(s) 495, 824, 1180, 1446, 1938, 3060, 3160, 3436
DinI GGCGCC 1 cut(s) 91
DraI TTTAAA 3 cut(s) 585, 2809, 2959
EaeI YGGCCR 2 cut(s) 57, 3237
Eam1104I CTCTTC 4 cut(s) 240, 1140, 3094, 3252
EarI CTCTTC 4 cut(s) 240, 1140, 3094, 3252
Eco130I CCWWGG 3 cut(s) 1583, 2519, 3245
Eco31I GGTCTC 2 cut(s) 1235, 3063
Eco47I GGWCC 5 cut(s) 1003, 1379, 1676, 2666, 3067
Eco57I CTGAAG 4 cut(s) 1172, 1797, 2064, 2511
Eco72I CACGTG 1 cut(s) 965
EcoNI CCTNNNNNAGG 1 cut(s) 2082
EcoO109I RGGNCCY 2 cut(s) 2129, 3407
EcoRI GAATTC 1 cut(s) 3473
EcoRII CCWGG 5 cut(s) 1084, 2668, 2779, 2801, 3403
EcoT14I CCWWGG 3 cut(s) 1583, 2519, 3245
EcoT22I ATGCAT 2 cut(s) 2833, 2925
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
ErhI CCWWGG 3 cut(s) 1583, 2519, 3245
FalI AAGNNNNNCTT 2 cut(s) 2084, 2116
FaqI GGGAC 1 cut(s) 2689
FauI CCCGC 1 cut(s) 595
FauNDI CATATG 3 cut(s) 652, 1245, 2265
FbaI TGATCA 1 cut(s) 2728
FblI GTMKAC 2 cut(s) 159, 1668
Fnu4HI GCNGC 7 cut(s) 504, 769, 1633, 2193, 2766, 3125, 3321
FokI GGATG 5 cut(s) 669, 674, 1301, 2354, 3458
Fsp4HI GCNGC 7 cut(s) 504, 769, 1633, 2193, 2766, 3125, 3321
FspBI CTAG 5 cut(s) 170, 407, 2520, 3201, 3663
GlaI GCGC 2 cut(s) 91, 1962
GluI GCNGC 7 cut(s) 504, 769, 1633, 2193, 2766, 3125, 3321
GsuI CTGGAG 4 cut(s) 18, 33, 1361, 2785
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 5 cut(s) 59, 768, 2131, 3239, 3409
HapII CCGG 3 cut(s) 897, 1123, 1286
HgaI GACGC 1 cut(s) 1952
HhaI GCGC 2 cut(s) 92, 1963
Hin1I GRCGYC 1 cut(s) 90
Hin6I GCGC 2 cut(s) 90, 1961
HinP1I GCGC 2 cut(s) 90, 1961
HincII GTYRAC 5 cut(s) 160, 578, 3085, 3370, 3519
HindII GTYRAC 5 cut(s) 160, 578, 3085, 3370, 3519
HindIII AAGCTT 1 cut(s) 821
HpaI GTTAAC 2 cut(s) 3085, 3519
HpaII CCGG 3 cut(s) 897, 1123, 1286
HphI GGTGA 6 cut(s) 395, 911, 1505, 2513, 3266, 3483
Hpy99I CGWCG 3 cut(s) 794, 861, 3232
HpyCH4III ACNGT 7 cut(s) 276, 599, 1499, 1562, 2071, 2158, 3228
HpyCH4IV ACGT 9 cut(s) 469, 698, 859, 964, 1161, 1225, 2104, 2401, 3372
HpyF10VI GCNNNNNNNGC 6 cut(s) 527, 1766, 2396, 2612, 2708, 2939
HpyF3I CTNAG 8 cut(s) 495, 824, 1180, 1446, 1938, 3060, 3160, 3436
HpySE526I ACGT 9 cut(s) 469, 698, 859, 964, 1161, 1225, 2104, 2401, 3372
Hsp92I GRCGYC 1 cut(s) 90
HspAI GCGC 2 cut(s) 90, 1961
KasI GGCGCC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 2728
KspAI GTTAAC 2 cut(s) 3085, 3519
LguI GCTCTTC 1 cut(s) 1140
LmnI GCTCC 1 cut(s) 3541
Lsp1109I GCAGC 4 cut(s) 490, 1644, 2777, 3332
MabI ACCWGGT 1 cut(s) 1084
MaeI CTAG 5 cut(s) 170, 407, 2520, 3201, 3663
MaeII ACGT 9 cut(s) 469, 698, 859, 964, 1161, 1225, 2104, 2401, 3372
MaeIII GTNAC 6 cut(s) 704, 774, 914, 1687, 1879, 2293
MflI RGATCY 3 cut(s) 209, 3137, 3417
MhlI GDGCHC 5 cut(s) 205, 1762, 2401, 2704, 3538
MlsI TGGCCA 1 cut(s) 3239
MluNI TGGCCA 1 cut(s) 3239
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 8 cut(s) 432, 500, 870, 878, 1475, 3269, 3428, 3705
MmeI TCCRAC 3 cut(s) 653, 2257, 3046
Mox20I TGGCCA 1 cut(s) 3239
Mph1103I ATGCAT 2 cut(s) 2833, 2925
MroXI GAANNNNTTC 1 cut(s) 3288
MscI TGGCCA 1 cut(s) 3239
Msp20I TGGCCA 1 cut(s) 3239
MspI CCGG 3 cut(s) 897, 1123, 1286
MspR9I CCNGG 7 cut(s) 1086, 1123, 1286, 2670, 2781, 2803, 3405
MvaI CCWGG 5 cut(s) 1086, 2670, 2781, 2803, 3405
MvnI CGCG 2 cut(s) 417, 1963
MwoI GCNNNNNNNGC 6 cut(s) 527, 1766, 2396, 2612, 2708, 2939
NarI GGCGCC 1 cut(s) 90
NciI CCSGG 2 cut(s) 1123, 1286
NcoI CCATGG 1 cut(s) 3245
NdeI CATATG 3 cut(s) 652, 1245, 2265
NlaIV GGNNCC 4 cut(s) 91, 211, 2913, 3419
NmeAIII GCCGAG 2 cut(s) 85, 3724
NmuCI GTSAC 2 cut(s) 704, 2293
NsiI ATGCAT 2 cut(s) 2833, 2925
NspI RCATGY 6 cut(s) 158, 1047, 1202, 1241, 1948, 2844
OliI CACNNNNGTG 1 cut(s) 3887
PciI ACATGT 3 cut(s) 1198, 1237, 1944
PciSI GCTCTTC 1 cut(s) 1140
PdmI GAANNNNTTC 1 cut(s) 3288
PflMI CCANNNNNTGG 1 cut(s) 3246
PfoI TCCNGGA 1 cut(s) 2668
PkrI GCNGC 7 cut(s) 505, 770, 1634, 2194, 2767, 3126, 3322
PleI GAGTC 8 cut(s) 432, 499, 870, 878, 1475, 3268, 3428, 3705
PluTI GGCGCC 1 cut(s) 93
PmaCI CACGTG 1 cut(s) 965
PmlI CACGTG 1 cut(s) 965
PpsI GAGTC 8 cut(s) 432, 499, 870, 878, 1475, 3268, 3428, 3705
Ppu21I YACGTR 3 cut(s) 965, 2105, 2402
PscI ACATGT 3 cut(s) 1198, 1237, 1944
PsiI TTATAA 4 cut(s) 120, 870, 3392, 3951
Psp6I CCWGG 5 cut(s) 1084, 2668, 2779, 2801, 3403
PspCI CACGTG 1 cut(s) 965
PspGI CCWGG 5 cut(s) 1084, 2668, 2779, 2801, 3403
PspN4I GGNNCC 4 cut(s) 91, 211, 2913, 3419
PspPI GGNCC 7 cut(s) 1003, 1379, 1676, 2129, 2666, 3067, 3407
PsrI GAACNNNNNNTAC 2 cut(s) 1872, 1904
PstI CTGCAG 2 cut(s) 76, 508
PstNI CAGNNNCTG 1 cut(s) 3410
PsuI RGATCY 3 cut(s) 209, 3137, 3417
SalI GTCGAC 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 1140
SatI GCNGC 7 cut(s) 504, 769, 1633, 2193, 2766, 3125, 3321
Sau96I GGNCC 7 cut(s) 1003, 1379, 1676, 2129, 2666, 3067, 3407
SchI GAGTC 8 cut(s) 432, 500, 870, 878, 1475, 3269, 3428, 3705
ScrFI CCNGG 7 cut(s) 1086, 1123, 1286, 2670, 2781, 2803, 3405
SduI GDGCHC 5 cut(s) 205, 1762, 2401, 2704, 3538
SexAI ACCWGGT 1 cut(s) 1084
SfcI CTRYAG 3 cut(s) 72, 486, 504
SfoI GGCGCC 1 cut(s) 91
SinI GGWCC 5 cut(s) 1003, 1379, 1676, 2666, 3067
SmlI CTYRAG 3 cut(s) 204, 2556, 2716
SmoI CTYRAG 3 cut(s) 204, 2556, 2716
SsiI CCGC 6 cut(s) 391, 602, 769, 1523, 2192, 3124
SspDI GGCGCC 1 cut(s) 89
SspI AATATT 3 cut(s) 109, 1782, 3559
SspMI CTAG 5 cut(s) 170, 407, 2520, 3201, 3663
StyD4I CCNGG 7 cut(s) 1084, 1121, 1284, 2668, 2779, 2801, 3403
StyI CCWWGG 3 cut(s) 1583, 2519, 3245
TaaI ACNGT 7 cut(s) 276, 599, 1499, 1562, 2071, 2158, 3228
TaiI ACGT 9 cut(s) 472, 701, 862, 967, 1164, 1228, 2107, 2404, 3375
TaqI TCGA 9 cut(s) 159, 760, 792, 1212, 1309, 1620, 1702, 1978, 3432
TatI WGTACW 7 cut(s) 301, 559, 1347, 1390, 1656, 2737, 3423
TauI GCSGC 3 cut(s) 771, 2195, 3127
TscAI CASTG 2 cut(s) 1821, 2401
TseFI GTSAC 2 cut(s) 704, 2293
TseI GCWGC 4 cut(s) 503, 1632, 2765, 3320
Tsp45I GTSAC 2 cut(s) 704, 2293
TspGWI ACGGA 3 cut(s) 1563, 2448, 3430
TspRI CASTG 2 cut(s) 1821, 2401
Van91I CCANNNNNTGG 1 cut(s) 3246
VneI GTGCAC 3 cut(s) 1758, 2397, 2700
VpaK11BI GGWCC 5 cut(s) 1003, 1379, 1676, 2666, 3067
XagI CCTNNNNNAGG 1 cut(s) 2082
XceI RCATGY 6 cut(s) 158, 1047, 1202, 1241, 1948, 2844
XcmI CCANNNNNNNNNTGG 2 cut(s) 1770, 2051
XmaJI CCTAGG 1 cut(s) 2519
XmiI GTMKAC 2 cut(s) 159, 1668
XmnI GAANNNNTTC 1 cut(s) 3288
XspI CTAG 5 cut(s) 170, 407, 2520, 3201, 3663
Zsp2I ATGCAT 2 cut(s) 2833, 2925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.