Prupe.4G273400_v2.0.a1

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000080
Physical Location & Seq
Reverse (-)
22612142 .. 22614984
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G273400.1

Sequence Viewer

Length: 2700 bp
ATGACTTTGGTTCAAAGATTTGGCAGGCCAGATCTATTCATTACCATGACATGCAACCCAAATTGGGAAGAAATTAAGAGAGAATTACTCCCTGGACAATCGCCGCAAGATCGTCCCGACTTACTCACTAGAGTATTTCATGCAAAATTGGAGGAACTAAAAAAAGACATAAATGGAAAAGGGGTATTAGGTAATGTTGCTGCTTATGCATACGTTATTGAGTTTCAAAAACGGGGTCTTCCACATGTGCATATGTTGGTTGTGTTAGATGAAAATGATAAGCTCAATAACCCAGATGATTATGACCAAATTGTTAAAGCTGAAATACCAAACGAACATGAAGAGCCTCACCTTCACAATGTGGTATTAAAACATATGATTCATGGTTCATGTGGAATTCAAAACCCAAAATCTCCCTGTATGAAAAATGGAAGGTGTAAAAGAAATTACCCTAAATCATTTGCTGCATCCACTATTCAACGAAATAAATCGCGAGGAAATTGCATGCCTGTCCCACTTGATCGACGAGGAAATATAACGGTTGATAATAGTTGGGTTTTTCCATATAATCCTTGGTTGTTGTTGAGGTATGACTGCCACATTGATGTTGAAATATGTGCAAGCATAAAAAGCATCAAATATTTATATAAGTATGTTTACAAAGGTCTAGACAGAGAGGCATTCGAGTTTGTTGATGCAAGATGGGTTTGTGCACTAGATGCATTATGGAGGATATTCAAGTTTGTAATCAATTGGATATACCCAACTGTGCAGCGCTTACAAATACATCTTCCTAATATGCATCAAGTTCGATTTCAATCTGACCAGACTATTGCAAATATTCTGATAGACGAGAGGCTTAACAAGACAATGCTCACTAAATTTTTCAGGGTTATTGGGCGAATATATACAGTCTCACCCACTGAAGGTGAGAAATTTTACCTACGCATACTTCTGAATCATGTCAGAGGAGCCACATCATTTCTAAATTTAATGACAATTGGTGGCGTTTTGCAACCAACGTTTAAGGAAGCAGCAGAACAACAAGGTTTGTTAGAAGAAGACGACAGTATTCGACAATGTTTGCTTGAGGCCTTTACAATTCGGATGCCATCGGCCTTAAGAAGATTATTTGTCACCATTTTGGTATATTGTGAACCAGTTGGTGTCCGGACATTGTGGGATGAATTCTATCCATTCATGATTGAAGATTATGCTTTCTCGAGTAACTTACGTTGCAATGTTATTGTGAACAGACTTTTACGTGACTTAAACACACTTTTGGTACAATTTAACAAGAATATCAAGGACTTTGATTTGCCCCAAATGACAACAAAAGTAGTTTCTGCAATGCCTAGATATATTGAAGACGAACTCTCAATAGGTATTTCACAAGAAGATCTTCTTGCAATTGAATGTTTTAATGACAACCAAAAGTCTGAATTTAACATAATAATGGACATAATTGAACGATGTCATAATTCAATTTTTTTTGTGACTGGTCCAGGTGGAACTGGAAAAACTTACTTATACCATGCATTGTTAGCAAACATGAGAGGGTTGGGTTATATAGTATTAGCAACATCAACATTTGGAATAGCAGCTACAATATTACCTAGTGGGAGAACAACACATTCTAGATTCAAGATACCACTTAGCACAGATTCATCATCTACATGTTCGATCAGTAAACAAGCTAATTTGGCAAAGTTAATACAACAGGCAAAGACAATTATTTGGGATGAATTAACGATGGCACATCGTTATGCCTTTGAAGCGCTAGATCGAACTTTTAGAGACATAATTGATGTTGACTTAACATTTGGGGGGAAAACAATGATCTTTGAGGGAGATTTTCAACAAGTACTTCCCGTTATTCCTAAAGGAAAGAAGTTGGAACTAATCCAGGCCAGTGTGATTTTGAAACTTAGACAAAACATGAGGAATGATCAACAATTTTCACAATTTCTACTTCATGTTGGTAATGGGGAAGCACTTGTTGTTGGAGATGATATGATAAGAGTACCTGAATGCATGGTAATACCATGGGAGAATGAGTTATCCATCAATGAATTTATTTATCAAGTTTTCCCAAACTTGGAGGATTACATGGTGGAAAGGGCAGTGATAAGTCCTACAAATGAAGATGTCGACATGTTGAATGAAAAGATGATCAATATGTTTCCAGGCAAAAAAAAGACAATGTATTCGTTTGATTCAATTGAGGCGGAATTCTTGAACTCAATTTGTATTGGTGGGTTGCCACCACATAAGTTAACTTTGAAGAGAGATGCTCCAATCATGCTTCTTAGAAATATTGACCCAACATTGGGATTGTATAATGGAACAAGATTATTATGTCGTGGGTCGTACCAAAATCTCATTGATGCTGAAATTCTAACTGGACAATCTGTTGGGACCAGGGTTTTTCTGCCATGTATCCCTCTAAAGACAACTGAGACTGCTGGGCTACCATTTGAACTAACAAGAAAACAATTTCCAATGAAGTTGTGTTTTGCTCTCAGTATAAATAAATCTCAAGATCACATTTTCAGTCATGGACAATTATACGTGGATTTATCGATGGATGTTTCAAAGTCGACTACAAAAGTGTTCGTGAAAAAAGGGTCAATAACCGGTCAAGAAGACGTTTTCACTTGGGATGTGATGTACAAAGAAGTCCTACTTCATTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

900

Amino Acids

103.11

Weight (kDa)

7.05

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 2151, 2600
AccII CGCG 1 cut(s) 493
AccIII TCCGGA 1 cut(s) 1170
AciI CCGC 2 cut(s) 104, 2228
AclI AACGTT 1 cut(s) 1022
AcsI RAATTY 9 cut(s) 396, 881, 935, 988, 1187, 1442, 2072, 2231, 2394
AcuI CTGAAG 1 cut(s) 945
AdeI CACNNNGTG 1 cut(s) 361
AfaI GTAC 5 cut(s) 1287, 1866, 2025, 2372, 2672
AfeI AGCGCT 2 cut(s) 776, 1779
AfiI CCNNNNNNNGG 5 cut(s) 64, 926, 2098, 2329, 2330
AflII CTTAAG 1 cut(s) 1120
AflIII ACRYGT 3 cut(s) 244, 1676, 2154
AgeI ACCGGT 1 cut(s) 2636
AjnI CCWGG 5 cut(s) 91, 1504, 1905, 2185, 2420
AluBI AGCT 4 cut(s) 283, 320, 1604, 1697
AluI AGCT 4 cut(s) 283, 320, 1604, 1697
Alw21I GWGCWC 1 cut(s) 715
Alw26I GTCTC 3 cut(s) 919, 1791, 2453
Alw44I GTGCAC 1 cut(s) 711
Ama87I CYCGRG 1 cut(s) 1222
Aor13HI TCCGGA 1 cut(s) 1170
Aor51HI AGCGCT 2 cut(s) 776, 1779
AoxI GGCC 4 cut(s) 26, 1092, 1116, 1908
ApaLI GTGCAC 1 cut(s) 711
ApeKI GCWGC 5 cut(s) 200, 464, 772, 1034, 1601
ApoI RAATTY 9 cut(s) 396, 881, 935, 988, 1187, 1442, 2072, 2231, 2394
ArsI GACNNNNNNTTYG 6 cut(s) 220, 252, 1805, 1837, 2191, 2223
AsiGI ACCGGT 1 cut(s) 2636
Asp700I GAANNNNTTC 1 cut(s) 1401
AspLEI GCGC 2 cut(s) 777, 1780
AspS9I GGNCC 2 cut(s) 1502, 2418
AsuHPI GGTGA 4 cut(s) 341, 909, 941, 1129
AvaI CYCGRG 1 cut(s) 1222
AvaII GGWCC 2 cut(s) 1502, 2418
BaeGI GKGCMC 1 cut(s) 715
BbsI GAAGAC 4 cut(s) 230, 1068, 1374, 2652
Bbv12I GWGCWC 1 cut(s) 715
BbvI GCAGC 5 cut(s) 187, 451, 784, 1046, 1613
BccI CCATC 5 cut(s) 696, 1120, 1747, 2072, 2578
BcgI CGANNNNNNTGC 4 cut(s) 483, 517, 791, 825
BciT130I CCWGG 5 cut(s) 93, 1506, 1907, 2187, 2422
BciVI GTATCC 1 cut(s) 2450
BclI TGATCA 2 cut(s) 1948, 2172
BcoDI GTCTC 3 cut(s) 919, 1791, 2453
BfaI CTAG 7 cut(s) 129, 668, 716, 1356, 1617, 1638, 1781
BfoI RGCGCY 2 cut(s) 778, 1781
BfrI CTTAAG 1 cut(s) 1120
BfuI GTATCC 1 cut(s) 2450
BglII AGATCT 2 cut(s) 31, 1399
BisI GCNGC 6 cut(s) 104, 201, 465, 773, 1035, 1602
BlsI GCNGC 6 cut(s) 105, 202, 466, 774, 1036, 1603
BmcAI AGTACT 1 cut(s) 1866
Bme1390I CCNGG 5 cut(s) 93, 1506, 1907, 2187, 2422
Bme18I GGWCC 2 cut(s) 1502, 2418
BmeT110I CYCGRG 1 cut(s) 1222
BmgT120I GGNCC 2 cut(s) 1502, 2418
BmiI GGNNCC 2 cut(s) 973, 2419
BmrFI CCNGG 5 cut(s) 93, 1506, 1907, 2187, 2422
BmsI GCATC 8 cut(s) 476, 642, 685, 709, 811, 1098, 2281, 2377
BpiI GAAGAC 4 cut(s) 230, 1068, 1374, 2652
BplI GAGNNNNNCTC 4 cut(s) 72, 104, 2278, 2310
BpuEI CTTGAG 2 cut(s) 1109, 2523
Bsa29I ATCGAT 1 cut(s) 2582
BsaAI YACGTR 2 cut(s) 1265, 2572
BsaBI GATNNNNATC 1 cut(s) 817
BsaJI CCNNGG 4 cut(s) 91, 572, 2045, 2421
BsaWI WCCGGW 2 cut(s) 1170, 2636
BsaXI ACNNNNNCTCC 2 cut(s) 2042, 2072
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 926, 2098, 2329, 2330
Bse118I RCCGGY 1 cut(s) 2636
Bse1I ACTGG 5 cut(s) 1160, 1504, 1519, 1911, 2407
Bse3DI GCAATG 2 cut(s) 1246, 1356
Bse8I GATNNNNATC 1 cut(s) 817
BseAI TCCGGA 1 cut(s) 1170
BseBI CCWGG 5 cut(s) 93, 1506, 1907, 2187, 2422
BseCI ATCGAT 1 cut(s) 2582
BseDI CCNNGG 4 cut(s) 91, 572, 2045, 2421
BseGI GGATG 6 cut(s) 467, 1113, 1189, 1747, 2593, 2668
BseJI GATNNNNATC 1 cut(s) 817
BseLI CCNNNNNNNGG 5 cut(s) 64, 926, 2098, 2329, 2330
BseMI GCAATG 2 cut(s) 1246, 1356
BseMII CTCAG 2 cut(s) 2448, 2536
BseNI ACTGG 5 cut(s) 1160, 1504, 1519, 1911, 2407
BseRI GAGGAG 1 cut(s) 984
BseSI GKGCMC 1 cut(s) 715
BseXI GCAGC 5 cut(s) 187, 451, 784, 1046, 1613
BseYI CCCAGC 1 cut(s) 2465
BsgI GTGCAG 1 cut(s) 791
Bsh1236I CGCG 1 cut(s) 493
BshFI GGCC 4 cut(s) 28, 1094, 1118, 1910
BshTI ACCGGT 1 cut(s) 2636
BshVI ATCGAT 1 cut(s) 2582
BsiHKAI GWGCWC 1 cut(s) 715
BsiHKCI CYCGRG 1 cut(s) 1222
BsiSI CCGG 2 cut(s) 1171, 2637
BslFI GGGAC 3 cut(s) 99, 497, 2431
BslI CCNNNNNNNGG 5 cut(s) 64, 926, 2098, 2329, 2330
BsmAI GTCTC 3 cut(s) 919, 1791, 2453
BsmFI GGGAC 3 cut(s) 99, 497, 2431
BsmI GAATGC 2 cut(s) 680, 2036
BsnI GGCC 4 cut(s) 28, 1094, 1118, 1910
BsoBI CYCGRG 1 cut(s) 1222
Bsp1286I GDGCHC 1 cut(s) 715
Bsp13I TCCGGA 1 cut(s) 1170
Bsp1407I TGTACA 1 cut(s) 2670
Bsp19I CCATGG 1 cut(s) 2045
Bsp68I TCGCGA 1 cut(s) 493
BspACI CCGC 2 cut(s) 104, 2228
BspANI GGCC 4 cut(s) 28, 1094, 1118, 1910
BspCNI CTCAG 2 cut(s) 2449, 2535
BspDI ATCGAT 1 cut(s) 2582
BspEI TCCGGA 1 cut(s) 1170
BspFNI CGCG 1 cut(s) 493
BspHI TCATGA 1 cut(s) 1200
BspLI GGNNCC 2 cut(s) 973, 2419
BspQI GCTCTTC 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 1120
BsrDI GCAATG 2 cut(s) 1246, 1356
BsrFI RCCGGY 1 cut(s) 2636
BsrGI TGTACA 1 cut(s) 2670
BsrI ACTGG 5 cut(s) 1160, 1504, 1519, 1911, 2407
BssAI RCCGGY 1 cut(s) 2636
BssECI CCNNGG 4 cut(s) 91, 572, 2045, 2421
BssT1I CCWWGG 2 cut(s) 572, 2045
Bst2UI CCWGG 5 cut(s) 93, 1506, 1907, 2187, 2422
Bst4CI ACNGT 4 cut(s) 541, 769, 913, 1070
Bst6I CTCTTC 2 cut(s) 336, 2279
BstAFI CTTAAG 1 cut(s) 1120
BstAPI GCANNNNNTGC 1 cut(s) 719
BstAUI TGTACA 1 cut(s) 2670
BstBAI YACGTR 2 cut(s) 1265, 2572
BstC8I GCNNGC 3 cut(s) 26, 506, 622
BstDEI CTNAG 5 cut(s) 1655, 1928, 2309, 2457, 2522
BstDSI CCRYGG 1 cut(s) 2045
BstF5I GGATG 6 cut(s) 467, 1113, 1189, 1747, 2593, 2668
BstFNI CGCG 1 cut(s) 493
BstH2I RGCGCY 2 cut(s) 778, 1781
BstHHI GCGC 2 cut(s) 777, 1780
BstMAI GTCTC 3 cut(s) 919, 1791, 2453
BstMWI GCNNNNNNNGC 6 cut(s) 206, 630, 719, 1544, 1703, 1775
BstNI CCWGG 5 cut(s) 93, 1506, 1907, 2187, 2422
BstNSI RCATGY 5 cut(s) 54, 248, 508, 1680, 2158
BstSCI CCNGG 5 cut(s) 91, 1504, 1905, 2185, 2420
BstSLI GKGCMC 1 cut(s) 715
BstUI CGCG 1 cut(s) 493
BstV1I GCAGC 5 cut(s) 187, 451, 784, 1046, 1613
BstV2I GAAGAC 4 cut(s) 230, 1068, 1374, 2652
BstX2I RGATCY 2 cut(s) 31, 1399
BstYI RGATCY 2 cut(s) 31, 1399
Bsu15I ATCGAT 1 cut(s) 2582
BsuI GTATCC 1 cut(s) 2450
BsuRI GGCC 4 cut(s) 28, 1094, 1118, 1910
BsuTUI ATCGAT 1 cut(s) 2582
BtgI CCRYGG 1 cut(s) 2045
BtsCI GGATG 6 cut(s) 467, 1113, 1189, 1747, 2593, 2668
BtsI GCAGTG 1 cut(s) 2130
BtsIMutI CAGTG 3 cut(s) 921, 1918, 2130
BtuMI TCGCGA 1 cut(s) 493
Cac8I GCNNGC 3 cut(s) 26, 506, 622
CciI TCATGA 1 cut(s) 1200
CfoI GCGC 2 cut(s) 777, 1780
Cfr10I RCCGGY 1 cut(s) 2636
Cfr13I GGNCC 2 cut(s) 1502, 2418
ClaI ATCGAT 1 cut(s) 2582
Csp6I GTAC 5 cut(s) 1286, 1865, 2024, 2371, 2671
CspAI ACCGGT 1 cut(s) 2636
CviQI GTAC 5 cut(s) 1286, 1865, 2024, 2371, 2671
DdeI CTNAG 5 cut(s) 1655, 1928, 2309, 2457, 2522
DraIII CACNNNGTG 1 cut(s) 361
Eam1104I CTCTTC 2 cut(s) 336, 2279
EarI CTCTTC 2 cut(s) 336, 2279
EciI GGCGGA 1 cut(s) 2243
Eco130I CCWWGG 2 cut(s) 572, 2045
Eco147I AGGCCT 1 cut(s) 1094
Eco47I GGWCC 2 cut(s) 1502, 2418
Eco47III AGCGCT 2 cut(s) 776, 1779
Eco57I CTGAAG 1 cut(s) 945
Eco88I CYCGRG 1 cut(s) 1222
EcoRI GAATTC 3 cut(s) 396, 1187, 2231
EcoRII CCWGG 5 cut(s) 91, 1504, 1905, 2185, 2420
EcoT14I CCWWGG 2 cut(s) 572, 2045
EcoT22I ATGCAT 5 cut(s) 211, 724, 804, 1540, 2036
ErhI CCWWGG 2 cut(s) 572, 2045
FalI AAGNNNNNCTT 5 cut(s) 1386, 1418, 1389, 1421, 2670
FaqI GGGAC 3 cut(s) 99, 497, 2431
FauNDI CATATG 2 cut(s) 252, 375
FbaI TGATCA 2 cut(s) 1948, 2172
FblI GTMKAC 2 cut(s) 2151, 2600
Fnu4HI GCNGC 6 cut(s) 104, 201, 465, 773, 1035, 1602
FokI GGATG 6 cut(s) 454, 1120, 1196, 1754, 2600, 2675
Fsp4HI GCNGC 6 cut(s) 104, 201, 465, 773, 1035, 1602
FspBI CTAG 7 cut(s) 129, 668, 716, 1356, 1617, 1638, 1781
GlaI GCGC 2 cut(s) 776, 1779
GluI GCNGC 6 cut(s) 104, 201, 465, 773, 1035, 1602
GsaI CCCAGC 1 cut(s) 2469
HaeII RGCGCY 2 cut(s) 778, 1781
HaeIII GGCC 4 cut(s) 28, 1094, 1118, 1910
HapII CCGG 2 cut(s) 1171, 2637
HhaI GCGC 2 cut(s) 777, 1780
Hin6I GCGC 2 cut(s) 775, 1778
HinP1I GCGC 2 cut(s) 775, 1778
HincII GTYRAC 4 cut(s) 1813, 2152, 2277, 2601
HindII GTYRAC 4 cut(s) 1813, 2152, 2277, 2601
HinfI GANTC 5 cut(s) 379, 958, 1641, 1664, 2216
HpaI GTTAAC 1 cut(s) 2277
HpaII CCGG 2 cut(s) 1171, 2637
HphI GGTGA 4 cut(s) 341, 909, 941, 1129
Hpy166II GTNNAC 9 cut(s) 658, 713, 1157, 1252, 1691, 1813, 2152, 2277, 2601
Hpy188I TCNGA 6 cut(s) 823, 846, 957, 968, 1107, 1441
Hpy8I GTNNAC 9 cut(s) 658, 713, 1157, 1252, 1691, 1813, 2152, 2277, 2601
Hpy99I CGWCG 1 cut(s) 528
HpyAV CCTTC 3 cut(s) 362, 426, 920
HpyCH4III ACNGT 4 cut(s) 541, 769, 913, 1070
HpyCH4IV ACGT 6 cut(s) 213, 1022, 1234, 1264, 2571, 2649
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 630, 719, 1544, 1703, 1775
HpyF3I CTNAG 5 cut(s) 1655, 1928, 2309, 2457, 2522
HpySE526I ACGT 6 cut(s) 213, 1022, 1234, 1264, 2571, 2649
HspAI GCGC 2 cut(s) 775, 1778
Kpn2I TCCGGA 1 cut(s) 1170
Ksp22I TGATCA 2 cut(s) 1948, 2172
KspAI GTTAAC 1 cut(s) 2277
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 2 cut(s) 971, 2299
Lsp1109I GCAGC 5 cut(s) 187, 451, 784, 1046, 1613
LweI GCATC 8 cut(s) 476, 642, 685, 709, 811, 1098, 2281, 2377
MaeI CTAG 7 cut(s) 129, 668, 716, 1356, 1617, 1638, 1781
MaeII ACGT 6 cut(s) 213, 1022, 1234, 1264, 2571, 2649
MaeIII GTNAC 4 cut(s) 1135, 1226, 1265, 1495
MfeI CAATTG 4 cut(s) 751, 999, 1410, 2220
MflI RGATCY 2 cut(s) 31, 1399
MhlI GDGCHC 1 cut(s) 715
MmeI TCCRAC 2 cut(s) 1875, 1984
Mph1103I ATGCAT 5 cut(s) 211, 724, 804, 1540, 2036
MroI TCCGGA 1 cut(s) 1170
MroXI GAANNNNTTC 1 cut(s) 1401
MslI CAYNNNNRTG 5 cut(s) 44, 603, 1454, 1675, 1764
MspCI CTTAAG 1 cut(s) 1120
MspI CCGG 2 cut(s) 1171, 2637
MspR9I CCNGG 5 cut(s) 93, 1506, 1907, 2187, 2422
MunI CAATTG 4 cut(s) 751, 999, 1410, 2220
Mva1269I GAATGC 2 cut(s) 680, 2036
MvaI CCWGG 5 cut(s) 93, 1506, 1907, 2187, 2422
MvnI CGCG 1 cut(s) 493
MwoI GCNNNNNNNGC 6 cut(s) 206, 630, 719, 1544, 1703, 1775
NcoI CCATGG 1 cut(s) 2045
NdeI CATATG 2 cut(s) 252, 375
NlaIV GGNNCC 2 cut(s) 973, 2419
NmuCI GTSAC 3 cut(s) 1135, 1265, 1495
NruI TCGCGA 1 cut(s) 493
NsiI ATGCAT 5 cut(s) 211, 724, 804, 1540, 2036
NspI RCATGY 5 cut(s) 54, 248, 508, 1680, 2158
PaeI GCATGC 1 cut(s) 508
PaeR7I CTCGAG 1 cut(s) 1222
PagI TCATGA 1 cut(s) 1200
PceI AGGCCT 1 cut(s) 1094
PciI ACATGT 3 cut(s) 244, 1676, 2154
PciSI GCTCTTC 1 cut(s) 336
PctI GAATGC 2 cut(s) 680, 2036
PdmI GAANNNNTTC 1 cut(s) 1401
PfeI GAWTC 5 cut(s) 379, 958, 1641, 1664, 2216
PinAI ACCGGT 1 cut(s) 2636
PkrI GCNGC 6 cut(s) 105, 202, 466, 774, 1036, 1603
Ppu21I YACGTR 2 cut(s) 1265, 2572
PscI ACATGT 3 cut(s) 244, 1676, 2154
Psp1406I AACGTT 1 cut(s) 1022
Psp6I CCWGG 5 cut(s) 91, 1504, 1905, 2185, 2420
PspFI CCCAGC 1 cut(s) 2465
PspGI CCWGG 5 cut(s) 91, 1504, 1905, 2185, 2420
PspN4I GGNNCC 2 cut(s) 973, 2419
PspPI GGNCC 2 cut(s) 1502, 2418
PsuI RGATCY 2 cut(s) 31, 1399
RruI TCGCGA 1 cut(s) 493
RsaI GTAC 5 cut(s) 1287, 1866, 2025, 2372, 2672
RsaNI GTAC 5 cut(s) 1286, 1865, 2024, 2371, 2671
RseI CAYNNNNRTG 5 cut(s) 44, 603, 1454, 1675, 1764
SalI GTCGAC 2 cut(s) 2150, 2599
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 6 cut(s) 104, 201, 465, 773, 1035, 1602
Sau96I GGNCC 2 cut(s) 1502, 2418
ScaI AGTACT 1 cut(s) 1866
ScrFI CCNGG 5 cut(s) 93, 1506, 1907, 2187, 2422
SduI GDGCHC 1 cut(s) 715
SfaNI GCATC 8 cut(s) 476, 642, 685, 709, 811, 1098, 2281, 2377
Sfr274I CTCGAG 1 cut(s) 1222
SinI GGWCC 2 cut(s) 1502, 2418
SlaI CTCGAG 1 cut(s) 1222
SmiMI CAYNNNNRTG 5 cut(s) 44, 603, 1454, 1675, 1764
SmlI CTYRAG 4 cut(s) 1088, 1120, 1222, 2538
SmoI CTYRAG 4 cut(s) 1088, 1120, 1222, 2538
SphI GCATGC 1 cut(s) 508
SseBI AGGCCT 1 cut(s) 1094
SsiI CCGC 2 cut(s) 104, 2228
SspI AATATT 4 cut(s) 641, 841, 1611, 2317
SspMI CTAG 7 cut(s) 129, 668, 716, 1356, 1617, 1638, 1781
StuI AGGCCT 1 cut(s) 1094
StyD4I CCNGG 5 cut(s) 91, 1504, 1905, 2185, 2420
StyI CCWWGG 2 cut(s) 572, 2045
TaaI ACNGT 4 cut(s) 541, 769, 913, 1070
TaiI ACGT 6 cut(s) 216, 1025, 1237, 1267, 2574, 2652
TatI WGTACW 2 cut(s) 1864, 2670
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 5 cut(s) 379, 958, 1641, 1664, 2216
TscAI CASTG 3 cut(s) 928, 1918, 2130
TseFI GTSAC 3 cut(s) 1135, 1265, 1495
TseI GCWGC 5 cut(s) 200, 464, 772, 1034, 1601
Tsp45I GTSAC 3 cut(s) 1135, 1265, 1495
TspRI CASTG 3 cut(s) 928, 1918, 2130
Vha464I CTTAAG 1 cut(s) 1120
VneI GTGCAC 1 cut(s) 711
VpaK11BI GGWCC 2 cut(s) 1502, 2418
XapI RAATTY 9 cut(s) 396, 881, 935, 988, 1187, 1442, 2072, 2231, 2394
XbaI TCTAGA 2 cut(s) 667, 1637
XceI RCATGY 5 cut(s) 54, 248, 508, 1680, 2158
XcmI CCANNNNNNNNNTGG 1 cut(s) 570
XhoI CTCGAG 1 cut(s) 1222
XmiI GTMKAC 2 cut(s) 2151, 2600
XmnI GAANNNNTTC 1 cut(s) 1401
XspI CTAG 7 cut(s) 129, 668, 716, 1356, 1617, 1638, 1781
ZrmI AGTACT 1 cut(s) 1866
Zsp2I ATGCAT 5 cut(s) 211, 724, 804, 1540, 2036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.