Rw3G024360

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000080
Physical Location & Seq
Reverse (-)
31274048 .. 31279337
5290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024360.1

Sequence Viewer

Length: 3846 bp
ATGTGTTGCTCCAATGGAAAGATTGTCTTGCCTCCCATACATTCTCCTCCAGATATGATCGAGCTATTCTCCTCTGAATCTCCACAAGGAGCCCATTTTAGGCAAAACATTCGAGCCTACAACCATGTATTTTCATTCACATCAATGGGTGTGCATGTAGATGAAAGTCTTGCAACCGGGGGTGGTACTTTCACATTTCGTGCTCAGGGATCCATATATCATAAGATAGGAAGTCTTCTACCAAATGTCAATGATAGACCAAGATATCTACAATTGTATATATATGATACTGACCATGAAGTGGACAATCGTATGCTGGAGAATGCAGCATTACATAGAGATGTGGTTGAAAAAATACAGAGAATTTTGAATCAACATAACCCATTTGTGCAAACATTTCATCACCTAGCTCAGCGTCCGGATTTGCAAAATTGCAGACTCATCATTAGAGAGCAAGCTGCAAACCAACATCAGTATAGTTTGCCTTCAGCATCCCAGGTTGCTGCAATAATAGTGGGTACAAATGATGTGGAAAATCTGACAGGTCGTGATATTGTTGTGCAAACAAAGCAAGGCCAGCTTCTAAATATTCGAGATTGTGTTGGGTACTATGATCCCTTACAGTATCCATTATTGTTCCCATATGGAACATATGGATGGGATGTGAATAGTAGGAATAATAATGGTCAAAAATTGACGTGTCGTGACTATTATGCGTATATTTTGCAGATCCGTCAATATGATGATTCACTTTTACTCCGAGGGGGCCGTCTATTGCAACAATATGTCGTGGATAATTACGTCAAAATTGAAACACAAAAGTTGAGATGGATTCGTAACATTGGTCGCAAGACTATTTTACCATCATCTTTTATTGGAAGTCCACGTGATATGCACCAAAGATATCAGGATGCAATGGCTTTAGTCCAAAAATATGGAAAACCGGATATTTTTCTCACAATGACATGTAATCCAAATTGGGAAGAAATAACTGCAGAGTTATTACCTGGTCAAGTGGCACAAGATCGCCCGGATCTAACTACTCGAATTTTTCATGCCAAATTTGAAGAGTTAAAGAATGATGTTATTAAAAAAGGAGTCTTGGGTACTATTGTTGCATACGTATATGTCATCGAGTTTCAAAAACGTGGTCTCCCACATGTTCATATGCTTCTCATGTTAAGTGACGATGATAAACTAAACAACCCAGATGATTATGACCACATTGTTCGGGCTGAAATTCCAAATCAAAATGAAGAGCCAGAGTTATACAATGCAGTGTTGGGGCACATGATACACGGTCCATGTGGGGTACATAGGCGAACTGCTCCATGTATGAAGCGTGGGAGCTGCAAACGAGGCTATCCAAAGCCATTTTCAGTAACCACATTTCAAGGAAATGATTCTTATCCTGTTTATCGTAGACGAGATAATCAATCAGATATGCAATCTAATGGCAATCAAAATAGACCACTGGATAATAGTTGGGTTATCCCCTATAATCCATGGTTACTCACAAAATATGATTGTCATATCGATGTGGAAATTTGTTGCAGCATTAAGAGCGTCAAGTATTTGTATAAATATGTCTACAAAGGTCCAGACAGAGTAACATTTGAAGTTCGGCCTGAAGCAAATCAAGATGAAATAAGAAACTTTGTTGATGCAAGATGGGTTTGTGCACCAGAAGCTTTGTGGAGGATGTTTAAATTCGTGATGAATCGAATGTATCCATCAGTGGAACGATTGCAAATTCATCTTCCAAATCGACACAATGTTTTCTTTAATGCCACTGAAAGTGTAACAGCAATTCTGGCAAATGAAAGAAGCTCGATGACAATGCTCACAGAATTTTTTACTAAGAATGCTTATTTTGAAAGTGCACGTCAATACTTATATCGAGAGTTTCCTGAGCATTACAAATGGGATGGGAGACTTAAAACATGGGAAGCAAGGGAGACCAACCAAAAAGTCATTGGAAGAATTTACACAGTGTCACCTTCAGAAGGAGAAAATTTTTACTTGCGTGTTCTTCTTAATCATGTTCGAGGACCTAAGTCATTTGAGGATTTAAGGACAGTTGATGGGGTCTTACAACCGACATTCAAGAAAGCAGTTGAAGAACGTGGTTTGTTAGAAGATGATGAAAGTATTAGACAATGTCTACGTGAGGCCTCTAATATACGTATGCCGTCATCTTTAAGAAGATTGTTTGTTACCATCCTGGTTTATTGCATGCCCCATGGAGTACGCAGTTTGTGGGATGAATTTTATCCATTCATGATAGAGGATTATCCTTCTTCGAGCACCGCGGACAATGTACGTATCACAAATAGACTTTTACGGGACTTGAACGAGTTGTTGGTGCAGCACAGTAAGTCCGTCTTTGATTATGACTTGCCAGAAATGACTCAAGATGGTGGTGAAAATTCTTCAATGCCAAGGCTAATACAAGATGAAATTTCAATTAACATTCCTCAAGAAGACCGGGATGCAGTTCATCATTTGAATGAGGATCAAACTTTTGCATACAATTCCATAATATCTGCTATTGAGTGTCATGACAATGCCATATTTTTTGTTGATGGTCCAGGAGGAACTGGAAAGACATACTTATATCGTGCATTATTAGCAAGCTTGAGAAGCAATGATCATATTGTATTGGCAACAGCAACTTCTGGCATAGCAGCAATAATACTACCTGGTGGGAGGACGGCACACTCTAGGTTTAAAATTCCTCTTAATCTTGATGCATCTTCAACATGTTGTATTAGTAAACAATCTGATTTAGCAGAGTTGATAAGAAAGTCATCGGCAATTGTTTGGGATGAAGCACCAATGATGAATCGATATGGGTTTGAAGCTCTTGATCGAACATTTAGAGACATAACAAGTGTCGATTTGCCATTTGGAGGAAAGGTGATGATCTTCGGGGGTGATTTCCGACAAGTTCTTCCTGTTATCCATAAGGGTACGAGATCTGAGATGGTGCAAGCCAGTTTGATTAATGCTTCATTTTGGAAGGATGTGAAGATTCTTCGTTTGACACAGAATATGAGATCCATCAACGATCCAGATTTCTCAGAGTTCTTACTTCGTGTTGGTAATGGAGAACAACCAACTGTGGTTGAAGACATAATACAAATACCTTGGCCCATGATCATACCATGGGAGAATGAAGAATCAATCAACCAGTTAGTTAATCAAGTCTTCCCTAATTTGGATCGTCATGTAAATGATGCCGGGTACATGGTTGAAAGAGCAATAATTACTCCAAAAAATGATGATGTTGACGTACTCAATGAAAGAATCATACAACACTTTCCAGGGCCTGAACGAATCTTATACTCTTTCGACTCAGTTGAGGATGACACCAGAAATATGTATCAACAAGAATTTTTAAATTCGATTTCACCTTCTGGTATGCCGCCACATCAGTTGATTATAAAGAAAGGTGCCCCAATTATGCTTTTGAGGAATATTGACCCAAAGATGGGGTTGTGCAACGGTACAAGATTGACTTGCCGCGGAGCATATAACAATCTAATTGATGCAGAGATTTTAACAGGACATTTTTCGGGAACTAGAATTTTCTTGCCAAGAATCGCTCTTAAGAGTGCCGAAAGTTCTGGACTCCCGTTTGAGATGACAAGAAAGCAGTTCCCCGTAAAGTTGAGTTTTGCACTTACCATAAATAAATCACAAGGCCAGACAATACCAAATGTTGGTATTTACCTTCCTGATCATGTGTTCAGCCATGGACAACTGTACGTGGCTTTATCGAGAGGATTAAAAAAAAAATTTTGTAGGAAATATGGATGGAAATTTTACCAAACTTTTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1281

Amino Acids

147.34

Weight (kDa)

6.98

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 279 - 392 5.7e-47 Helitron helicase-like domain at N-terminus
PIF1 PF05970 836 - 1041 9.5e-76 PIF1-like helicase
Pif1_2B_dom PF21530 1132 - 1174 1.7e-19 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3446, 3844
AccB1I GGYRCC 1 cut(s) 3455
AccB7I CCANNNNNTGG 2 cut(s) 301, 3725
AccI GTMKAC 3 cut(s) 1423, 1590, 2164
AccII CGCG 2 cut(s) 2312, 3528
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 5 cut(s) 2310, 2312, 3428, 3526, 3528
AclWI GGATC 9 cut(s) 204, 217, 608, 724, 1041, 2523, 3054, 3065, 3231
AcuI CTGAAG 3 cut(s) 471, 1650, 1986
AcvI CACGTG 1 cut(s) 887
AfiI CCNNNNNNNGG 8 cut(s) 99, 301, 2006, 2912, 3220, 3493, 3494, 3725
AflII CTTAAG 1 cut(s) 3611
AflIII ACRYGT 4 cut(s) 698, 965, 1159, 2762
AjiI CACGTC 2 cut(s) 699, 1886
AjnI CCWGG 6 cut(s) 495, 1006, 2223, 2590, 2701, 3325
AjuI GAANNNNNNNTTGG 4 cut(s) 1360, 1392, 2345, 2377
AluBI AGCT 9 cut(s) 64, 410, 458, 580, 1350, 1691, 1830, 2637, 2864
AluI AGCT 9 cut(s) 64, 410, 458, 580, 1350, 1691, 1830, 2637, 2864
Alw21I GWGCWC 4 cut(s) 205, 1684, 1885, 2309
Alw26I GTCTC 4 cut(s) 1157, 1927, 1952, 2877
Alw44I GTGCAC 2 cut(s) 1680, 1881
AlwI GGATC 9 cut(s) 204, 217, 608, 724, 1041, 2523, 3054, 3065, 3231
AlwNI CAGNNNCTG 1 cut(s) 3332
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 7 cut(s) 574, 766, 1625, 2172, 3152, 3329, 3706
ApaLI GTGCAC 2 cut(s) 1680, 1881
ApeKI GCWGC 7 cut(s) 326, 458, 503, 1350, 1554, 2368, 2687
ArsI GACNNNNNNTTYG 2 cut(s) 1135, 1167
AseI ATTAAT 1 cut(s) 3006
Asp700I GAANNNNTTC 1 cut(s) 1402
AspS9I GGNCC 7 cut(s) 766, 1301, 1598, 2051, 2588, 3153, 3329
AsuC2I CCSGG 4 cut(s) 178, 1031, 2489, 3244
AsuHPI GGTGA 6 cut(s) 395, 1989, 2435, 2932, 2948, 3405
AvaII GGWCC 4 cut(s) 1301, 1598, 2051, 2588
BaeGI GKGCMC 4 cut(s) 1290, 1684, 1885, 3460
BaeI ACNNNNGTAYC 2 cut(s) 1098, 1131
BamHI GGATCC 1 cut(s) 209
BanI GGYRCC 1 cut(s) 3455
BanII GRGCYC 1 cut(s) 94
BarI GAAGNNNNNNTAC 2 cut(s) 3123, 3155
BbrPI CACGTG 1 cut(s) 887
BbsI GAAGAC 4 cut(s) 227, 2490, 3138, 3202
Bbv12I GWGCWC 4 cut(s) 205, 1684, 1885, 2309
BbvI GCAGC 7 cut(s) 338, 445, 490, 1337, 1566, 2380, 2699
BceAI ACGGC 3 cut(s) 753, 2176, 2730
BcgI CGANNNNNNTGC 4 cut(s) 92, 126, 1822, 1856
BciT130I CCWGG 6 cut(s) 497, 1008, 2225, 2592, 2703, 3327
BciVI GTATCC 2 cut(s) 636, 1740
BclI TGATCA 3 cut(s) 2650, 3159, 3742
BcnI CCSGG 4 cut(s) 178, 1031, 2489, 3244
BcoDI GTCTC 4 cut(s) 1157, 1927, 1952, 2877
BfaI CTAG 3 cut(s) 407, 2724, 3585
BfmI CTRYAG 1 cut(s) 993
BfrI CTTAAG 1 cut(s) 3611
BfuI GTATCC 2 cut(s) 636, 1740
BglII AGATCT 1 cut(s) 2978
BisI GCNGC 9 cut(s) 327, 459, 504, 1351, 1555, 2369, 2688, 3428, 3526
BlpI GCTNAGC 1 cut(s) 411
BlsI GCNGC 9 cut(s) 328, 460, 505, 1352, 1556, 2370, 2689, 3429, 3527
Bme18I GGWCC 4 cut(s) 1301, 1598, 2051, 2588
BmgBI CACGTC 2 cut(s) 699, 1886
BmgT120I GGNCC 7 cut(s) 766, 1301, 1598, 2051, 2588, 3153, 3329
BmiI GGNNCC 4 cut(s) 91, 211, 767, 3457
BmsI GCATC 8 cut(s) 500, 901, 1654, 2482, 2740, 2762, 3229, 3541
BoxI GACNNNNGTC 1 cut(s) 2056
BpiI GAAGAC 4 cut(s) 227, 2490, 3138, 3202
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpmI CTGGAG 2 cut(s) 33, 338
Bpu10I CCTNAGC 2 cut(s) 204, 1911
Bpu1102I GCTNAGC 1 cut(s) 411
BpuEI CTTGAG 3 cut(s) 2397, 2463, 2659
BpuMI CCSGG 4 cut(s) 178, 1031, 2489, 3244
Bsa29I ATCGAT 2 cut(s) 1536, 2848
BsaAI YACGTR 6 cut(s) 887, 1123, 2168, 2186, 2324, 3772
BsaBI GATNNNNATC 1 cut(s) 1407
BsaI GGTCTC 2 cut(s) 1157, 1952
BsaWI WCCGGW 2 cut(s) 418, 943
BsaXI ACNNNNNCTCC 6 cut(s) 741, 771, 1137, 1167, 2904, 2934
Bsc4I CCNNNNNNNGG 8 cut(s) 99, 301, 2006, 2912, 3220, 3493, 3494, 3725
Bse1I ACTGG 4 cut(s) 1479, 2605, 2997, 3193
Bse3DI GCAATG 2 cut(s) 921, 2653
Bse8I GATNNNNATC 1 cut(s) 1407
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 6 cut(s) 497, 1008, 2225, 2592, 2703, 3327
BseCI ATCGAT 2 cut(s) 1536, 2848
BseJI GATNNNNATC 1 cut(s) 1407
BseLI CCNNNNNNNGG 8 cut(s) 99, 301, 2006, 2912, 3220, 3493, 3494, 3725
BseMI GCAATG 2 cut(s) 921, 2653
BseMII CTCAG 6 cut(s) 218, 425, 1902, 2973, 3096, 3372
BseNI ACTGG 4 cut(s) 1479, 2605, 2997, 3193
BseRI GAGGAG 2 cut(s) 36, 61
BseSI GKGCMC 4 cut(s) 1290, 1684, 1885, 3460
BseXI GCAGC 7 cut(s) 338, 445, 490, 1337, 1566, 2380, 2699
BsgI GTGCAG 1 cut(s) 2387
Bsh1236I CGCG 2 cut(s) 2312, 3528
BshFI GGCC 7 cut(s) 576, 768, 1627, 2174, 3154, 3331, 3708
BshNI GGYRCC 1 cut(s) 3455
BshVI ATCGAT 2 cut(s) 1536, 2848
BsiHKAI GWGCWC 4 cut(s) 205, 1684, 1885, 2309
BsiSI CCGG 6 cut(s) 177, 419, 944, 1031, 2488, 3243
BslFI GGGAC 1 cut(s) 2360
BslI CCNNNNNNNGG 8 cut(s) 99, 301, 2006, 2912, 3220, 3493, 3494, 3725
BsmAI GTCTC 4 cut(s) 1157, 1927, 1952, 2877
BsmFI GGGAC 1 cut(s) 2360
BsmI GAATGC 2 cut(s) 328, 1870
BsnI GGCC 7 cut(s) 576, 768, 1627, 2174, 3154, 3331, 3708
Bso31I GGTCTC 2 cut(s) 1157, 1952
Bsp1286I GDGCHC 7 cut(s) 94, 205, 1290, 1684, 1885, 2309, 3460
Bsp13I TCCGGA 1 cut(s) 418
Bsp1720I GCTNAGC 1 cut(s) 411
Bsp19I CCATGG 4 cut(s) 1505, 2242, 3167, 3757
BspACI CCGC 5 cut(s) 2310, 2312, 3428, 3526, 3528
BspANI GGCC 7 cut(s) 576, 768, 1627, 2174, 3154, 3331, 3708
BspCNI CTCAG 6 cut(s) 217, 424, 1903, 2974, 3095, 3371
BspDI ATCGAT 2 cut(s) 1536, 2848
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 2 cut(s) 2312, 3528
BspHI TCATGA 2 cut(s) 2280, 2560
BspLI GGNNCC 4 cut(s) 91, 211, 767, 3457
BspMAI CTGCAG 1 cut(s) 997
BspPI GGATC 9 cut(s) 204, 217, 608, 724, 1041, 2523, 3054, 3065, 3231
BspQI GCTCTTC 1 cut(s) 1251
BspT107I GGYRCC 1 cut(s) 3455
BspTI CTTAAG 1 cut(s) 3611
BspTNI GGTCTC 2 cut(s) 1157, 1952
BsrDI GCAATG 2 cut(s) 921, 2653
BsrI ACTGG 4 cut(s) 1479, 2605, 2997, 3193
BssT1I CCWWGG 6 cut(s) 1505, 2242, 2441, 3149, 3167, 3757
Bst2UI CCWGG 6 cut(s) 497, 1008, 2225, 2592, 2703, 3327
Bst4CI ACNGT 8 cut(s) 624, 1301, 1993, 2080, 2375, 3124, 3509, 3768
Bst6I CTCTTC 2 cut(s) 1062, 1251
BstAFI CTTAAG 1 cut(s) 3611
BstBAI YACGTR 6 cut(s) 887, 1123, 2168, 2186, 2324, 3772
BstC8I GCNNGC 5 cut(s) 456, 578, 2237, 2635, 2994
BstDEI CTNAG 8 cut(s) 204, 411, 1860, 1911, 2055, 2982, 3082, 3358
BstDSI CCRYGG 6 cut(s) 1505, 2242, 2310, 3167, 3526, 3757
BstENI CCTNNNNNAGG 1 cut(s) 2004
BstFNI CGCG 2 cut(s) 2312, 3528
BstMAI GTCTC 4 cut(s) 1157, 1927, 1952, 2877
BstMWI GCNNNNNNNGC 8 cut(s) 568, 577, 1359, 1563, 1688, 1814, 2630, 2643
BstNI CCWGG 6 cut(s) 497, 1008, 2225, 2592, 2703, 3327
BstNSI RCATGY 5 cut(s) 158, 969, 1163, 2239, 2766
BstPAI GACNNNNGTC 1 cut(s) 2056
BstSFI CTRYAG 1 cut(s) 993
BstSLI GKGCMC 4 cut(s) 1290, 1684, 1885, 3460
BstSNI TACGTA 3 cut(s) 1123, 2186, 2324
BstUI CGCG 2 cut(s) 2312, 3528
BstV1I GCAGC 7 cut(s) 338, 445, 490, 1337, 1566, 2380, 2699
BstV2I GAAGAC 4 cut(s) 227, 2490, 3138, 3202
BstX2I RGATCY 5 cut(s) 209, 729, 1033, 2978, 3059
BstXI CCANNNNNNTGG 1 cut(s) 935
BstYI RGATCY 5 cut(s) 209, 729, 1033, 2978, 3059
Bsu15I ATCGAT 2 cut(s) 1536, 2848
BsuI GTATCC 2 cut(s) 636, 1740
BsuRI GGCC 7 cut(s) 576, 768, 1627, 2174, 3154, 3331, 3708
BsuTUI ATCGAT 2 cut(s) 1536, 2848
BtgI CCRYGG 6 cut(s) 1505, 2242, 2310, 3167, 3526, 3757
BtrI CACGTC 2 cut(s) 699, 1886
BtsI GCAGTG 1 cut(s) 1284
BtsIMutI CAGTG 5 cut(s) 1284, 1472, 1743, 1791, 1998
Cac8I GCNNGC 5 cut(s) 456, 578, 2237, 2635, 2994
CaiI CAGNNNCTG 1 cut(s) 3332
CciI TCATGA 2 cut(s) 2280, 2560
Cfr13I GGNCC 7 cut(s) 766, 1301, 1598, 2051, 2588, 3153, 3329
Cfr42I CCGCGG 2 cut(s) 2313, 3529
ClaI ATCGAT 2 cut(s) 1536, 2848
CseI GACGC 2 cut(s) 404, 1555
CsiI ACCWGGT 2 cut(s) 1006, 2701
CspCI CAANNNNNGTGG 6 cut(s) 495, 510, 530, 545, 3420, 3455
DdeI CTNAG 8 cut(s) 204, 411, 1860, 1911, 2055, 2982, 3082, 3358
DraI TTTAAA 3 cut(s) 1708, 2731, 3402
Eam1104I CTCTTC 2 cut(s) 1062, 1251
EarI CTCTTC 2 cut(s) 1062, 1251
Eco105I TACGTA 3 cut(s) 1123, 2186, 2324
Eco130I CCWWGG 6 cut(s) 1505, 2242, 2441, 3149, 3167, 3757
Eco147I AGGCCT 1 cut(s) 2174
Eco24I GRGCYC 1 cut(s) 94
Eco31I GGTCTC 2 cut(s) 1157, 1952
Eco32I GATATC 2 cut(s) 266, 905
Eco47I GGWCC 4 cut(s) 1301, 1598, 2051, 2588
Eco57I CTGAAG 3 cut(s) 471, 1650, 1986
Eco72I CACGTG 1 cut(s) 887
EcoNI CCTNNNNNAGG 1 cut(s) 2004
EcoO109I RGGNCCY 2 cut(s) 2051, 3329
EcoRII CCWGG 6 cut(s) 495, 1006, 2223, 2590, 2701, 3325
EcoRV GATATC 2 cut(s) 266, 905
EcoT14I CCWWGG 6 cut(s) 1505, 2242, 2441, 3149, 3167, 3757
EcoT22I ATGCAT 1 cut(s) 2755
EcoT38I GRGCYC 1 cut(s) 94
ErhI CCWWGG 6 cut(s) 1505, 2242, 2441, 3149, 3167, 3757
FaqI GGGAC 1 cut(s) 2360
FauNDI CATATG 3 cut(s) 643, 652, 1167
FbaI TGATCA 3 cut(s) 2650, 3159, 3742
FblI GTMKAC 3 cut(s) 1423, 1590, 2164
Fnu4HI GCNGC 9 cut(s) 327, 459, 504, 1351, 1555, 2369, 2688, 3428, 3526
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 9 cut(s) 327, 459, 504, 1351, 1555, 2369, 2688, 3428, 3526
FspBI CTAG 3 cut(s) 407, 2724, 3585
GluI GCNGC 9 cut(s) 327, 459, 504, 1351, 1555, 2369, 2688, 3428, 3526
GsuI CTGGAG 2 cut(s) 33, 338
HaeIII GGCC 7 cut(s) 576, 768, 1627, 2174, 3154, 3331, 3708
HapII CCGG 6 cut(s) 177, 419, 944, 1031, 2488, 3243
HgaI GACGC 2 cut(s) 404, 1555
HincII GTYRAC 1 cut(s) 3292
HindII GTYRAC 1 cut(s) 3292
HindIII AAGCTT 2 cut(s) 1689, 2635
HpaII CCGG 6 cut(s) 177, 419, 944, 1031, 2488, 3243
HphI GGTGA 6 cut(s) 395, 1989, 2435, 2932, 2948, 3405
Hpy166II GTNNAC 9 cut(s) 304, 884, 1424, 1591, 1682, 1883, 2165, 2777, 3292
Hpy188I TCNGA 9 cut(s) 76, 540, 761, 1441, 2005, 2785, 2945, 2983, 3085
Hpy8I GTNNAC 9 cut(s) 304, 884, 1424, 1591, 1682, 1883, 2165, 2777, 3292
HpyAV CCTTC 7 cut(s) 495, 2000, 2010, 2307, 3016, 3426, 3746
HpyCH4III ACNGT 8 cut(s) 624, 1301, 1993, 2080, 2375, 3124, 3509, 3768
HpyF10VI GCNNNNNNNGC 8 cut(s) 568, 577, 1359, 1563, 1688, 1814, 2630, 2643
HpyF3I CTNAG 8 cut(s) 204, 411, 1860, 1911, 2055, 2982, 3082, 3358
Kpn2I TCCGGA 1 cut(s) 418
Ksp22I TGATCA 3 cut(s) 2650, 3159, 3742
KspI CCGCGG 2 cut(s) 2313, 3529
LguI GCTCTTC 1 cut(s) 1251
LmnI GCTCC 5 cut(s) 14, 89, 1333, 1347, 3530
Lsp1109I GCAGC 7 cut(s) 338, 445, 490, 1337, 1566, 2380, 2699
LweI GCATC 8 cut(s) 500, 901, 1654, 2482, 2740, 2762, 3229, 3541
MabI ACCWGGT 2 cut(s) 1006, 2701
MaeI CTAG 3 cut(s) 407, 2724, 3585
MaeIII GTNAC 9 cut(s) 704, 836, 1184, 1381, 1509, 1609, 1801, 1995, 2215
MfeI CAATTG 2 cut(s) 272, 2817
MflI RGATCY 5 cut(s) 209, 729, 1033, 2978, 3059
MhlI GDGCHC 7 cut(s) 94, 205, 1290, 1684, 1885, 2309, 3460
MlyI GAGTC 5 cut(s) 432, 1107, 2404, 3350, 3627
MmeI TCCRAC 1 cut(s) 2968
Mph1103I ATGCAT 1 cut(s) 2755
MroI TCCGGA 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 1402
MslI CAYNNNNRTG 8 cut(s) 143, 159, 339, 655, 1536, 2508, 2565, 3234
MspA1I CMGCKG 2 cut(s) 2312, 3528
MspCI CTTAAG 1 cut(s) 3611
MspI CCGG 6 cut(s) 177, 419, 944, 1031, 2488, 3243
MunI CAATTG 2 cut(s) 272, 2817
Mva1269I GAATGC 2 cut(s) 328, 1870
MvaI CCWGG 6 cut(s) 497, 1008, 2225, 2592, 2703, 3327
MvnI CGCG 2 cut(s) 2312, 3528
MwoI GCNNNNNNNGC 8 cut(s) 568, 577, 1359, 1563, 1688, 1814, 2630, 2643
NciI CCSGG 4 cut(s) 178, 1031, 2489, 3244
NcoI CCATGG 4 cut(s) 1505, 2242, 3167, 3757
NdeI CATATG 3 cut(s) 643, 652, 1167
NlaIV GGNNCC 4 cut(s) 91, 211, 767, 3457
NmuCI GTSAC 3 cut(s) 704, 1184, 1995
NsiI ATGCAT 1 cut(s) 2755
NspI RCATGY 5 cut(s) 158, 969, 1163, 2239, 2766
PaeI GCATGC 1 cut(s) 2239
PagI TCATGA 2 cut(s) 2280, 2560
PceI AGGCCT 1 cut(s) 2174
PciI ACATGT 3 cut(s) 965, 1159, 2762
PciSI GCTCTTC 1 cut(s) 1251
PctI GAATGC 2 cut(s) 328, 1870
PdmI GAANNNNTTC 1 cut(s) 1402
PflFI GACNNNGTC 1 cut(s) 2160
PflMI CCANNNNNTGG 2 cut(s) 301, 3725
PfoI TCCNGGA 1 cut(s) 2590
PkrI GCNGC 9 cut(s) 328, 460, 505, 1352, 1556, 2370, 2689, 3429, 3527
PleI GAGTC 5 cut(s) 432, 1106, 2404, 3350, 3627
PmaCI CACGTG 1 cut(s) 887
PmlI CACGTG 1 cut(s) 887
PpsI GAGTC 5 cut(s) 432, 1106, 2404, 3350, 3627
Ppu21I YACGTR 6 cut(s) 887, 1123, 2168, 2186, 2324, 3772
PpuMI RGGWCCY 1 cut(s) 2051
PscI ACATGT 3 cut(s) 965, 1159, 2762
PshAI GACNNNNGTC 1 cut(s) 2056
PshBI ATTAAT 1 cut(s) 3006
PsiI TTATAA 2 cut(s) 3446, 3844
Psp5II RGGWCCY 1 cut(s) 2051
Psp6I CCWGG 6 cut(s) 495, 1006, 2223, 2590, 2701, 3325
PspCI CACGTG 1 cut(s) 887
PspGI CCWGG 6 cut(s) 495, 1006, 2223, 2590, 2701, 3325
PspN4I GGNNCC 4 cut(s) 91, 211, 767, 3457
PspPI GGNCC 7 cut(s) 766, 1301, 1598, 2051, 2588, 3153, 3329
PspPPI RGGWCCY 1 cut(s) 2051
PstI CTGCAG 1 cut(s) 997
PstNI CAGNNNCTG 1 cut(s) 3332
PsuI RGATCY 5 cut(s) 209, 729, 1033, 2978, 3059
PsyI GACNNNGTC 1 cut(s) 2160
RseI CAYNNNNRTG 8 cut(s) 143, 159, 339, 655, 1536, 2508, 2565, 3234
SacII CCGCGG 2 cut(s) 2313, 3529
SapI GCTCTTC 1 cut(s) 1251
SatI GCNGC 9 cut(s) 327, 459, 504, 1351, 1555, 2369, 2688, 3428, 3526
Sau96I GGNCC 7 cut(s) 766, 1301, 1598, 2051, 2588, 3153, 3329
SchI GAGTC 5 cut(s) 432, 1107, 2404, 3350, 3627
SduI GDGCHC 7 cut(s) 94, 205, 1290, 1684, 1885, 2309, 3460
SexAI ACCWGGT 2 cut(s) 1006, 2701
SfaNI GCATC 8 cut(s) 500, 901, 1654, 2482, 2740, 2762, 3229, 3541
SfcI CTRYAG 1 cut(s) 993
Sfr303I CCGCGG 2 cut(s) 2313, 3529
SgrBI CCGCGG 2 cut(s) 2313, 3529
SinI GGWCC 4 cut(s) 1301, 1598, 2051, 2588
SmiMI CAYNNNNRTG 8 cut(s) 143, 159, 339, 655, 1536, 2508, 2565, 3234
SmlI CTYRAG 4 cut(s) 2412, 2478, 2638, 3611
SmoI CTYRAG 4 cut(s) 2412, 2478, 2638, 3611
SnaBI TACGTA 3 cut(s) 1123, 2186, 2324
SphI GCATGC 1 cut(s) 2239
SseBI AGGCCT 1 cut(s) 2174
SsiI CCGC 5 cut(s) 2310, 2312, 3428, 3526, 3528
SspI AATATT 2 cut(s) 589, 3481
SspMI CTAG 3 cut(s) 407, 2724, 3585
StuI AGGCCT 1 cut(s) 2174
StyI CCWWGG 6 cut(s) 1505, 2242, 2441, 3149, 3167, 3757
TaaI ACNGT 8 cut(s) 624, 1301, 1993, 2080, 2375, 3124, 3509, 3768
TauI GCSGC 2 cut(s) 3430, 3528
TscAI CASTG 5 cut(s) 1284, 1479, 1743, 1798, 1998
TseFI GTSAC 3 cut(s) 704, 1184, 1995
TseI GCWGC 7 cut(s) 326, 458, 503, 1350, 1554, 2368, 2687
Tsp45I GTSAC 3 cut(s) 704, 1184, 1995
TspGWI ACGGA 2 cut(s) 722, 2371
TspRI CASTG 5 cut(s) 1284, 1479, 1743, 1798, 1998
Tth111I GACNNNGTC 1 cut(s) 2160
Van91I CCANNNNNTGG 2 cut(s) 301, 3725
Vha464I CTTAAG 1 cut(s) 3611
VneI GTGCAC 2 cut(s) 1680, 1881
VpaK11BI GGWCC 4 cut(s) 1301, 1598, 2051, 2588
VspI ATTAAT 1 cut(s) 3006
XagI CCTNNNNNAGG 1 cut(s) 2004
XceI RCATGY 5 cut(s) 158, 969, 1163, 2239, 2766
XcmI CCANNNNNNNNNTGG 2 cut(s) 1692, 1973
XmiI GTMKAC 3 cut(s) 1423, 1590, 2164
XmnI GAANNNNTTC 1 cut(s) 1402
XspI CTAG 3 cut(s) 407, 2724, 3585
Zsp2I ATGCAT 1 cut(s) 2755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.