FvH4_5g24553

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000080
Physical Location & Seq
Forward (+)
15932543 .. 15935757
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24553.t1

Sequence Viewer

Length: 2649 bp
ATGGCTTTGGTTCAAAAATATGGGAGGCCAGATCTTTTTATCACCATGACATGTAACCCAAATTGGGAAGAAGTTAAAAGTCAACTGCTCCAAGGACAAGTGCCACAAGATTGCCCTGATCTGGTCACCAGGGTATTTCACGCAAAATTGGAACAATTGAAAGAAGACGTAATTAAAAAAGGGGTGCTTGGAAAAGTAGCCGCACATGCTTTCGTTGTGGAATTTCAAAAACGAGGCTTACCGCATGTTCACATGCTGATTATGTTAGAACCAAATGACAAGCTTAACAACCCTGATGAATATGATCGCATTGTTAAAGCTGAAATACCAGATCAGAATGAAGAGCCTCGACTATATGATGCAGTGTGTACACATATGATTCATGGCCCATGTGGACCTCTCAATCCACGACAATCATGTATCAAAAAAGGCACTTGCAATAAGGGTTACCCAAAATCATTTGCGAATTTCACAGTGCAAGGCAAAGATGCATATCCTATGTATCAAAGACGACCAAACCGTCAACCAGTATATTTGCGTGGTCAAGGAAATATGACTGTGGATAACCGCTGGGTGGTTCCCTACAACCCATGGTTGTTATTACGGTATAATTGTCACATCAATGTCGAAGTATGCGGTAGTATAAAGAGTGTCAAGTACTTATATAAGTATATCTACAAAGGTCCTGACAGAGTGTCACTTGAGGTGCAGTCCAACCCAGAGTTCGATGAAATTAGCCAATTTGTTGATGCAAGGTGGGTTATAGGGAGAATATACAGCGTGTCCCCCTCTGAAGGTGAGAAATTTTACTTACGTGTTCTTTTGAATCATGTAAGAGGTCCAAAATCATTTTTGGACTTGCTTACTGTTAATGGGGCTTTACAACCAACATTCAAGCAGGCAGCGGAGAACCGAGGTTTGCTTGAAAATGACAATAGTATTCGAATGTGTCTGGCGGAGGCATCTGCAATAAGGATGCCATCAGCTTTGAGGAGATTATTCGTTACACTCTTGGTGTATGGGGTGCCAGTTGGTGTTCGAGCATTGTGGGATGAATTTGCGCATTTTATGGTAGAAGATTATACTTCATCTGATGATATTGCAAACAGAAACAGGCTCTTACGAGACCTAAATAGAATACTTCAACAATTTGGCAAGAACATTAGAGATTATGACCTCCCTCAATTAACCGGTGAGCTAGAAGATAATATCGGAATGCCAAGATGTATTGAAGATGAACTTTTTATCCATGTTCCTCAAGAAGATATTGATGCAATTGATCGTCTTAACAATGACCAAATGGTTGCCTTCAACACCATAATGTCTGTTGTGGATGGAAAAGAAAGTGCTTTATTTTTTGTTGATGGTCCCGGAGGAACTGGGAAAACGTATCTATACCGTGCGCTGTTAGCAAATTTAAGAAGAATGAATCATATAGTCTTAGCAACAACATCATTTGGAATAGCGGCAAACATATTGCCCGGCGGGAGGACAGCTCACTCCAGATTCAAGATTCCCCTAAAAGTACTACCTTCATCCATGTGTTCCATAGGTAAACAGTCTGCACTGGCACAATTGGTACGACAATCCACTGCAATTATATGGGATGAGGCACCTATGACAAATCGAGATGTGTTTGAATTAGTTGATCGAACTTTTCAGGACATAATGGACGTGAAAATTCCTTTTGGTGGTAAAATAATGATTTTCGGGGGAGATTTTCGTCAAGTCCTTCCTGTCATTCCTAAAGGAACAAAGTCTCAACTCATCGAAGCCTCGATTATGAGATCATCGTTTTGGCCAGCTACAAGGATATTGAGACTGAGGCAGAATATGAGATCCATAAATGACCACCGATTTGCGGAGTTTCTATTACGTGTTGGCGACGGCGATGCAAGATACATGGTGGATAGAGCATTGATTACTCCTTTGAACGACGATGCAGACATGTTGAATGAAAAGATCATCAGTATGTTTCCTGGTGAAGAAATTTCATTTTACTCATTTGACTCTGTTGAAGATGACATCCGAAATCTGTACCAGCCTGAGTTTTTAAATTCAATAACCGCAAGCGGTTTACCGCCGCACAGGTTAACTCTAAAAACAGGTGCTCTAATCATGCTCTTGAGAAATATAGACCCAAAATTAGGGTTGTGTAATGGTACTAGATTGTTATGTCGTGGTTCCTACCAGAATTTGATTGATGCAGAAATTATGACAGGCGAGTTTGTTGGGTCTAGAGTTTTTCTGTCAAGAATTCCTTTGCAAAGTGCAGAAAATGCGGGACTACTGTTTCAACTTACAAGAAAGCAATTTCCAGTCAAGTTGAGCTTTGCTTTGACCATAAATAAATCTCAAGGTCAAACCATCCCACATGTTGGAGTTTACCTCCCAGATCATGTTTTTAGTCATGGACAATTATATGTGGCATTGTCGAGAGGAGTATCAGAGTCTACCACAAAGGTGTTGGTTAAAAAAGGGTCCATAGTTGGTGAAGATGGCATATTTACTAAGAACGTCGTAAGTACTGTGACATGGAAGCTCTCAACTACAGAAGAAGCTACAAATAGGCGATGGCTTCAGGCGGCATGTTTCTTTATTTTCATGTGGTTGGCACAAGGGAATATCAGTGACAATGAACACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

883

Amino Acids

99.96

Weight (kDa)

8.55

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 1 - 87 3.6e-34 Helitron helicase-like domain at N-terminus
PIF1 PF05970 429 - 633 1.7e-68 PIF1-like helicase
Pif1_2B_dom PF21530 683 - 725 4.6e-20 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 519
Acc16I TGCGCA 1 cut(s) 1062
AccB1I GGYRCC 2 cut(s) 1024, 1612
AccB7I CCANNNNNTGG 1 cut(s) 2378
AccI GTMKAC 1 cut(s) 2453
AclWI GGATC 1 cut(s) 1833
AcoI YGGCCR 1 cut(s) 1799
AcuI CTGAAG 2 cut(s) 813, 2564
AfaI GTAC 7 cut(s) 370, 659, 1527, 1581, 2039, 2164, 2527
AfiI CCNNNNNNNGG 9 cut(s) 64, 121, 574, 794, 1488, 1691, 1861, 2147, 2378
AflIII ACRYGT 5 cut(s) 50, 814, 1876, 1947, 2374
AgeI ACCGGT 1 cut(s) 1190
AhdI GACNNNNNGTC 1 cut(s) 694
AjiI CACGTC 1 cut(s) 1675
AjnI CCWGG 2 cut(s) 128, 1978
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
AleI CACNNNNGTG 1 cut(s) 2462
AloI GAACNNNNNNTCC 2 cut(s) 1230, 1262
AluBI AGCT 9 cut(s) 283, 320, 986, 1198, 1496, 1805, 2331, 2542, 2561
AluI AGCT 9 cut(s) 283, 320, 986, 1198, 1496, 1805, 2331, 2542, 2561
Alw21I GWGCWC 1 cut(s) 2113
Alw26I GTCTC 3 cut(s) 1119, 1764, 1813
AlwI GGATC 1 cut(s) 1833
AoxI GGCC 3 cut(s) 26, 385, 1799
ApeKI GCWGC 1 cut(s) 902
ArsI GACNNNNNNTTYG 2 cut(s) 1841, 1873
AsiGI ACCGGT 1 cut(s) 1190
AspLEI GCGC 2 cut(s) 1063, 1405
AspS9I GGNCC 6 cut(s) 386, 395, 683, 839, 1367, 2481
AsuC2I CCSGG 2 cut(s) 1371, 1482
AsuHPI GGTGA 6 cut(s) 34, 118, 809, 1205, 1994, 2504
AsuII TTCGAA 1 cut(s) 943
AvaII GGWCC 5 cut(s) 395, 683, 839, 1367, 2481
BalI TGGCCA 1 cut(s) 1801
BanI GGYRCC 2 cut(s) 1024, 1612
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 2113
BbvI GCAGC 1 cut(s) 914
BccI CCATC 6 cut(s) 988, 1328, 1358, 2375, 2492, 2568
BceAI ACGGC 1 cut(s) 1903
BcgI CGANNNNNNTGC 2 cut(s) 1874, 1908
BciT130I CCWGG 2 cut(s) 130, 1980
BcnI CCSGG 2 cut(s) 1371, 1482
BcoDI GTCTC 3 cut(s) 1119, 1764, 1813
BfaI CTAG 3 cut(s) 1199, 2166, 2238
BfmI CTRYAG 1 cut(s) 2550
BglII AGATCT 1 cut(s) 31
BisI GCNGC 5 cut(s) 201, 903, 1467, 2084, 2586
BlsI GCNGC 5 cut(s) 202, 904, 1468, 2085, 2587
BmcAI AGTACT 3 cut(s) 659, 1527, 2527
Bme1390I CCNGG 4 cut(s) 130, 1371, 1482, 1980
Bme18I GGWCC 5 cut(s) 395, 683, 839, 1367, 2481
BmeRI GACNNNNNGTC 1 cut(s) 694
BmgBI CACGTC 1 cut(s) 1675
BmgT120I GGNCC 6 cut(s) 386, 395, 683, 839, 1367, 2481
BmiI GGNNCC 6 cut(s) 579, 1026, 1369, 1614, 2185, 2482
BmrFI CCNGG 4 cut(s) 130, 1371, 1482, 1980
BmrI ACTGGG 1 cut(s) 1389
BmsI GCATC 9 cut(s) 349, 478, 739, 966, 971, 1261, 1882, 1930, 2194
BmuI ACTGGG 1 cut(s) 1389
BpiI GAAGAC 1 cut(s) 171
BplI GAGNNNNNCTC 4 cut(s) 1480, 1512, 2373, 2405
BpmI CTGGAG 1 cut(s) 1486
Bpu14I TTCGAA 1 cut(s) 943
BpuEI CTTGAG 4 cut(s) 722, 1242, 2146, 2340
BpuMI CCSGG 2 cut(s) 1371, 1482
BsaAI YACGTR 2 cut(s) 815, 1877
BsaBI GATNNNNATC 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 1119
BsaJI CCNNGG 4 cut(s) 91, 129, 590, 913
BsaWI WCCGGW 1 cut(s) 1190
Bsc4I CCNNNNNNNGG 9 cut(s) 64, 121, 574, 794, 1488, 1691, 1861, 2147, 2378
Bse118I RCCGGY 1 cut(s) 1190
Bse1I ACTGG 5 cut(s) 527, 1028, 1384, 1572, 2318
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 2 cut(s) 130, 1980
BseDI CCNNGG 4 cut(s) 91, 129, 590, 913
BseGI GGATG 7 cut(s) 981, 1057, 1339, 1535, 1612, 2025, 2367
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 9 cut(s) 64, 121, 574, 794, 1488, 1691, 1861, 2147, 2378
BseMII CTCAG 2 cut(s) 1814, 2037
BseNI ACTGG 5 cut(s) 527, 1028, 1384, 1572, 2318
BseRI GAGGAG 2 cut(s) 1006, 2454
BseXI GCAGC 1 cut(s) 914
BseYI CCCAGC 1 cut(s) 570
BsgI GTGCAG 3 cut(s) 728, 1548, 2292
BshFI GGCC 3 cut(s) 28, 387, 1801
BshNI GGYRCC 2 cut(s) 1024, 1612
BshTI ACCGGT 1 cut(s) 1190
BsiHKAI GWGCWC 1 cut(s) 2113
BsiSI CCGG 3 cut(s) 1191, 1371, 1482
BslFI GGGAC 3 cut(s) 769, 1353, 2298
BslI CCNNNNNNNGG 9 cut(s) 64, 121, 574, 794, 1488, 1691, 1861, 2147, 2378
BsmAI GTCTC 3 cut(s) 1119, 1764, 1813
BsmFI GGGAC 3 cut(s) 769, 1353, 2298
BsmI GAATGC 1 cut(s) 1221
BsnI GGCC 3 cut(s) 28, 387, 1801
Bso31I GGTCTC 1 cut(s) 1119
Bsp119I TTCGAA 1 cut(s) 943
Bsp1286I GDGCHC 1 cut(s) 2113
Bsp1407I TGTACA 1 cut(s) 368
Bsp19I CCATGG 1 cut(s) 590
BspANI GGCC 3 cut(s) 28, 387, 1801
BspCNI CTCAG 2 cut(s) 1815, 2038
BspLI GGNNCC 6 cut(s) 579, 1026, 1369, 1614, 2185, 2482
BspPI GGATC 1 cut(s) 1833
BspQI GCTCTTC 1 cut(s) 336
BspT104I TTCGAA 1 cut(s) 943
BspT107I GGYRCC 2 cut(s) 1024, 1612
BspTNI GGTCTC 1 cut(s) 1119
BsrFI RCCGGY 1 cut(s) 1190
BsrGI TGTACA 1 cut(s) 368
BsrI ACTGG 5 cut(s) 527, 1028, 1384, 1572, 2318
BssAI RCCGGY 1 cut(s) 1190
BssECI CCNNGG 4 cut(s) 91, 129, 590, 913
BssT1I CCWWGG 2 cut(s) 91, 590
Bst2UI CCWGG 2 cut(s) 130, 1980
Bst6I CTCTTC 1 cut(s) 336
BstAPI GCANNNNNTGC 1 cut(s) 2279
BstAUI TGTACA 1 cut(s) 368
BstBAI YACGTR 2 cut(s) 815, 1877
BstBI TTCGAA 1 cut(s) 943
BstC8I GCNNGC 3 cut(s) 900, 1803, 2071
BstDEI CTNAG 4 cut(s) 1441, 1823, 2046, 2511
BstDSI CCRYGG 1 cut(s) 590
BstEII GGTNACC 2 cut(s) 124, 446
BstF5I GGATG 7 cut(s) 981, 1057, 1339, 1535, 1612, 2025, 2367
BstHHI GCGC 2 cut(s) 1063, 1405
BstMAI GTCTC 3 cut(s) 1119, 1764, 1813
BstMWI GCNNNNNNNGC 3 cut(s) 206, 1409, 2279
BstNI CCWGG 2 cut(s) 130, 1980
BstNSI RCATGY 7 cut(s) 54, 209, 248, 256, 1951, 2378, 2592
BstPI GGTNACC 2 cut(s) 124, 446
BstSCI CCNGG 4 cut(s) 128, 1369, 1480, 1978
BstSFI CTRYAG 1 cut(s) 2550
BstV1I GCAGC 1 cut(s) 914
BstV2I GAAGAC 1 cut(s) 171
BstX2I RGATCY 2 cut(s) 31, 1838
BstYI RGATCY 2 cut(s) 31, 1838
BsuRI GGCC 3 cut(s) 28, 387, 1801
BtgI CCRYGG 1 cut(s) 590
BtgZI GCGATG 2 cut(s) 1905, 2587
BtrI CACGTC 1 cut(s) 1675
BtsCI GGATG 7 cut(s) 981, 1057, 1339, 1535, 1612, 2025, 2367
BtsI GCAGTG 2 cut(s) 369, 1590
BtsIMutI CAGTG 5 cut(s) 369, 480, 1565, 1590, 2635
Cac8I GCNNGC 3 cut(s) 900, 1803, 2071
CfoI GCGC 2 cut(s) 1063, 1405
Cfr10I RCCGGY 1 cut(s) 1190
Cfr13I GGNCC 6 cut(s) 386, 395, 683, 839, 1367, 2481
Csp6I GTAC 7 cut(s) 369, 658, 1526, 1580, 2038, 2163, 2526
CspAI ACCGGT 1 cut(s) 1190
CviQI GTAC 7 cut(s) 369, 658, 1526, 1580, 2038, 2163, 2526
DdeI CTNAG 4 cut(s) 1441, 1823, 2046, 2511
DraI TTTAAA 1 cut(s) 2055
DrdI GACNNNNNNGTC 1 cut(s) 519
DriI GACNNNNNGTC 1 cut(s) 694
DseDI GACNNNNNNGTC 1 cut(s) 519
EaeI YGGCCR 1 cut(s) 1799
Eam1104I CTCTTC 1 cut(s) 336
Eam1105I GACNNNNNGTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 336
EciI GGCGGA 1 cut(s) 971
Eco130I CCWWGG 2 cut(s) 91, 590
Eco31I GGTCTC 1 cut(s) 1119
Eco47I GGWCC 5 cut(s) 395, 683, 839, 1367, 2481
Eco57I CTGAAG 2 cut(s) 813, 2564
Eco91I GGTNACC 2 cut(s) 124, 446
EcoO109I RGGNCCY 1 cut(s) 683
EcoO65I GGTNACC 2 cut(s) 124, 446
EcoRI GAATTC 1 cut(s) 2256
EcoRII CCWGG 2 cut(s) 128, 1978
EcoT14I CCWWGG 2 cut(s) 91, 590
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 2 cut(s) 91, 590
FalI AAGNNNNNCTT 8 cut(s) 171, 203, 1224, 1256, 2245, 2277, 2315, 2347
FaqI GGGAC 3 cut(s) 769, 1353, 2298
FauI CCCGC 2 cut(s) 1478, 2275
FauNDI CATATG 1 cut(s) 375
FblI GTMKAC 1 cut(s) 2453
Fnu4HI GCNGC 5 cut(s) 201, 903, 1467, 2084, 2586
FokI GGATG 7 cut(s) 988, 1064, 1346, 1522, 1619, 2012, 2354
Fsp4HI GCNGC 5 cut(s) 201, 903, 1467, 2084, 2586
FspBI CTAG 3 cut(s) 1199, 2166, 2238
FspI TGCGCA 1 cut(s) 1062
GlaI GCGC 2 cut(s) 1062, 1404
GluI GCNGC 5 cut(s) 201, 903, 1467, 2084, 2586
GsaI CCCAGC 1 cut(s) 574
GsuI CTGGAG 1 cut(s) 1486
HaeIII GGCC 3 cut(s) 28, 387, 1801
HapII CCGG 3 cut(s) 1191, 1371, 1482
HhaI GCGC 2 cut(s) 1063, 1405
Hin6I GCGC 2 cut(s) 1061, 1403
HinP1I GCGC 2 cut(s) 1061, 1403
HincII GTYRAC 3 cut(s) 83, 524, 2094
HindII GTYRAC 3 cut(s) 83, 524, 2094
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 7 cut(s) 379, 826, 1429, 1506, 1513, 2009, 2450
HpaI GTTAAC 1 cut(s) 2094
HpaII CCGG 3 cut(s) 1191, 1371, 1482
HphI GGTGA 6 cut(s) 34, 118, 809, 1205, 1994, 2504
Hpy188I TCNGA 6 cut(s) 336, 793, 1093, 1214, 2030, 2449
Hpy99I CGWCG 3 cut(s) 1889, 1940, 2522
HpyAV CCTTC 4 cut(s) 788, 1318, 1542, 1742
HpyCH4IV ACGT 6 cut(s) 168, 814, 1388, 1674, 1876, 2517
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 1409, 2279
HpyF3I CTNAG 4 cut(s) 1441, 1823, 2046, 2511
HpySE526I ACGT 6 cut(s) 168, 814, 1388, 1674, 1876, 2517
HspAI GCGC 2 cut(s) 1061, 1403
KspAI GTTAAC 1 cut(s) 2094
LguI GCTCTTC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 93
Lsp1109I GCAGC 1 cut(s) 914
LweI GCATC 9 cut(s) 349, 478, 739, 966, 971, 1261, 1882, 1930, 2194
MaeI CTAG 3 cut(s) 1199, 2166, 2238
MaeII ACGT 6 cut(s) 168, 814, 1388, 1674, 1876, 2517
MaeIII GTNAC 8 cut(s) 53, 124, 446, 614, 696, 1003, 2530, 2630
MfeI CAATTG 3 cut(s) 155, 1275, 1574
MflI RGATCY 2 cut(s) 31, 1838
MhlI GDGCHC 1 cut(s) 2113
MlsI TGGCCA 1 cut(s) 1801
MluNI TGGCCA 1 cut(s) 1801
MlyI GAGTC 2 cut(s) 2003, 2459
MmeI TCCRAC 2 cut(s) 738, 2359
Mox20I TGGCCA 1 cut(s) 1801
Mph1103I ATGCAT 1 cut(s) 493
MscI TGGCCA 1 cut(s) 1801
MslI CAYNNNNRTG 5 cut(s) 621, 1319, 1540, 1970, 2462
Msp20I TGGCCA 1 cut(s) 1801
MspA1I CMGCKG 2 cut(s) 570, 905
MspI CCGG 3 cut(s) 1191, 1371, 1482
MspR9I CCNGG 4 cut(s) 130, 1371, 1482, 1980
MunI CAATTG 3 cut(s) 155, 1275, 1574
Mva1269I GAATGC 1 cut(s) 1221
MvaI CCWGG 2 cut(s) 130, 1980
MwoI GCNNNNNNNGC 3 cut(s) 206, 1409, 2279
NciI CCSGG 2 cut(s) 1371, 1482
NcoI CCATGG 1 cut(s) 590
NdeI CATATG 1 cut(s) 375
NlaIV GGNNCC 6 cut(s) 579, 1026, 1369, 1614, 2185, 2482
NmuCI GTSAC 5 cut(s) 124, 614, 696, 2530, 2630
NsbI TGCGCA 1 cut(s) 1062
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 7 cut(s) 54, 209, 248, 256, 1951, 2378, 2592
NspV TTCGAA 1 cut(s) 943
OliI CACNNNNGTG 1 cut(s) 2462
PciI ACATGT 3 cut(s) 50, 1947, 2374
PciSI GCTCTTC 1 cut(s) 336
PcsI WCGNNNNNNNCGW 1 cut(s) 517
PctI GAATGC 1 cut(s) 1221
PfeI GAWTC 5 cut(s) 379, 826, 1429, 1506, 1513
PflMI CCANNNNNTGG 1 cut(s) 2378
PfoI TCCNGGA 1 cut(s) 1369
PinAI ACCGGT 1 cut(s) 1190
PkrI GCNGC 5 cut(s) 202, 904, 1468, 2085, 2587
PleI GAGTC 2 cut(s) 2003, 2458
PpsI GAGTC 2 cut(s) 2003, 2458
Ppu21I YACGTR 2 cut(s) 815, 1877
PpuMI RGGWCCY 1 cut(s) 683
PscI ACATGT 3 cut(s) 50, 1947, 2374
Psp5II RGGWCCY 1 cut(s) 683
Psp6I CCWGG 2 cut(s) 128, 1978
PspEI GGTNACC 2 cut(s) 124, 446
PspFI CCCAGC 1 cut(s) 570
PspGI CCWGG 2 cut(s) 128, 1978
PspN4I GGNNCC 6 cut(s) 579, 1026, 1369, 1614, 2185, 2482
PspPI GGNCC 6 cut(s) 386, 395, 683, 839, 1367, 2481
PspPPI RGGWCCY 1 cut(s) 683
PsuI RGATCY 2 cut(s) 31, 1838
RsaI GTAC 7 cut(s) 370, 659, 1527, 1581, 2039, 2164, 2527
RsaNI GTAC 7 cut(s) 369, 658, 1526, 1580, 2038, 2163, 2526
RseI CAYNNNNRTG 5 cut(s) 621, 1319, 1540, 1970, 2462
SapI GCTCTTC 1 cut(s) 336
SatI GCNGC 5 cut(s) 201, 903, 1467, 2084, 2586
Sau96I GGNCC 6 cut(s) 386, 395, 683, 839, 1367, 2481
ScaI AGTACT 3 cut(s) 659, 1527, 2527
SchI GAGTC 2 cut(s) 2003, 2459
ScrFI CCNGG 4 cut(s) 130, 1371, 1482, 1980
SduI GDGCHC 1 cut(s) 2113
SfaNI GCATC 9 cut(s) 349, 478, 739, 966, 971, 1261, 1882, 1930, 2194
SfcI CTRYAG 1 cut(s) 2550
SfuI TTCGAA 1 cut(s) 943
SinI GGWCC 5 cut(s) 395, 683, 839, 1367, 2481
SmiMI CAYNNNNRTG 5 cut(s) 621, 1319, 1540, 1970, 2462
SmlI CTYRAG 4 cut(s) 701, 1257, 2125, 2355
SmoI CTYRAG 4 cut(s) 701, 1257, 2125, 2355
SspMI CTAG 3 cut(s) 1199, 2166, 2238
StyD4I CCNGG 4 cut(s) 128, 1369, 1480, 1978
StyI CCWWGG 2 cut(s) 91, 590
TaiI ACGT 6 cut(s) 171, 817, 1391, 1677, 1879, 2520
TatI WGTACW 4 cut(s) 368, 657, 1525, 2525
TauI GCSGC 4 cut(s) 203, 1469, 2086, 2588
TfiI GAWTC 5 cut(s) 379, 826, 1429, 1506, 1513
TscAI CASTG 5 cut(s) 369, 480, 1572, 1597, 2635
TseFI GTSAC 5 cut(s) 124, 614, 696, 2530, 2630
TseI GCWGC 1 cut(s) 902
Tsp45I GTSAC 5 cut(s) 124, 614, 696, 2530, 2630
TspRI CASTG 5 cut(s) 369, 480, 1572, 1597, 2635
Van91I CCANNNNNTGG 1 cut(s) 2378
VpaK11BI GGWCC 5 cut(s) 395, 683, 839, 1367, 2481
XbaI TCTAGA 1 cut(s) 2237
XceI RCATGY 7 cut(s) 54, 209, 248, 256, 1951, 2378, 2592
XcmI CCANNNNNNNNNTGG 1 cut(s) 2464
XmiI GTMKAC 1 cut(s) 2453
XspI CTAG 3 cut(s) 1199, 2166, 2238
ZrmI AGTACT 3 cut(s) 659, 1527, 2527
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.