Belongs to the helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 585 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 516 |
| AccB1I | GGYRCC | 1 cut(s) | 1810 |
| AccI | GTMKAC | 2 cut(s) | 675, 825 |
| AciI | CCGC | 3 cut(s) | 307, 1487, 1557 |
| AclI | AACGTT | 1 cut(s) | 1171 |
| AcoI | YGGCCR | 1 cut(s) | 1554 |
| AcuI | CTGAAG | 1 cut(s) | 315 |
| AfaI | GTAC | 7 cut(s) | 249, 1609, 1622, 1997, 2036, 2161, 2186 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 64, 1016, 1719, 2143, 2144 |
| AflIII | ACRYGT | 4 cut(s) | 50, 244, 1036, 1828 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 743 |
| AjiI | CACGTC | 1 cut(s) | 51 |
| AjnI | CCWGG | 2 cut(s) | 91, 1600 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2031, 2063 |
| AleI | CACNNNNGTG | 1 cut(s) | 557 |
| AluBI | AGCT | 5 cut(s) | 85, 283, 1208, 2328, 2364 |
| AluI | AGCT | 5 cut(s) | 85, 283, 1208, 2328, 2364 |
| Alw21I | GWGCWC | 2 cut(s) | 769, 2110 |
| Alw26I | GTCTC | 3 cut(s) | 543, 875, 1854 |
| Alw44I | GTGCAC | 1 cut(s) | 765 |
| AoxI | GGCC | 2 cut(s) | 235, 1554 |
| ApaLI | GTGCAC | 1 cut(s) | 765 |
| ApeKI | GCWGC | 1 cut(s) | 2074 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 707, 739, 1694, 1726 |
| AseI | ATTAAT | 1 cut(s) | 1092 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 894 |
| AspS9I | GGNCC | 5 cut(s) | 235, 386, 683, 1061, 1598 |
| AsuC2I | CCSGG | 1 cut(s) | 719 |
| AsuHPI | GGTGA | 4 cut(s) | 934, 970, 999, 1031 |
| AsuII | TTCGAA | 1 cut(s) | 1261 |
| AsuNHI | GCTAGC | 1 cut(s) | 1639 |
| AvaII | GGWCC | 4 cut(s) | 386, 683, 1061, 1598 |
| BaeGI | GKGCMC | 2 cut(s) | 769, 1394 |
| BanI | GGYRCC | 1 cut(s) | 1810 |
| BbsI | GAAGAC | 6 cut(s) | 171, 405, 514, 897, 1470, 1622 |
| Bbv12I | GWGCWC | 2 cut(s) | 769, 2110 |
| BbvI | GCAGC | 1 cut(s) | 2061 |
| BccI | CCATC | 3 cut(s) | 1210, 1589, 1904 |
| BciT130I | CCWGG | 2 cut(s) | 93, 1602 |
| BciVI | GTATCC | 1 cut(s) | 825 |
| BcnI | CCSGG | 1 cut(s) | 719 |
| BcoDI | GTCTC | 3 cut(s) | 543, 875, 1854 |
| BfuI | GTATCC | 1 cut(s) | 825 |
| BglII | AGATCT | 2 cut(s) | 542, 1357 |
| BisI | GCNGC | 2 cut(s) | 1557, 2075 |
| BlsI | GCNGC | 2 cut(s) | 1558, 2076 |
| BmcAI | AGTACT | 1 cut(s) | 2161 |
| Bme1390I | CCNGG | 3 cut(s) | 93, 719, 1602 |
| Bme18I | GGWCC | 4 cut(s) | 386, 683, 1061, 1598 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 743 |
| BmgBI | CACGTC | 1 cut(s) | 51 |
| BmgT120I | GGNCC | 5 cut(s) | 235, 386, 683, 1061, 1598 |
| BmiI | GGNNCC | 4 cut(s) | 236, 397, 1812, 1968 |
| BmrFI | CCNGG | 3 cut(s) | 93, 719, 1602 |
| BmrI | ACTGGG | 1 cut(s) | 1620 |
| BmsI | GCATC | 7 cut(s) | 425, 478, 739, 1170, 1492, 1691, 2191 |
| BmtI | GCTAGC | 1 cut(s) | 1643 |
| BmuI | ACTGGG | 1 cut(s) | 1620 |
| BpiI | GAAGAC | 6 cut(s) | 171, 405, 514, 897, 1470, 1622 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1711, 1743 |
| BpmI | CTGGAG | 3 cut(s) | 567, 1416, 1623 |
| Bpu14I | TTCGAA | 1 cut(s) | 1261 |
| BpuEI | CTTGAG | 2 cut(s) | 170, 2337 |
| BpuMI | CCSGG | 1 cut(s) | 719 |
| Bsa29I | ATCGAT | 1 cut(s) | 1593 |
| BsaAI | YACGTR | 2 cut(s) | 539, 1037 |
| BsaBI | GATNNNNATC | 1 cut(s) | 492 |
| BsaJI | CCNNGG | 3 cut(s) | 91, 590, 1970 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 64, 1016, 1719, 2143, 2144 |
| Bse1I | ACTGG | 5 cut(s) | 527, 1258, 1399, 1615, 2315 |
| Bse3DI | GCAATG | 1 cut(s) | 1634 |
| Bse8I | GATNNNNATC | 1 cut(s) | 492 |
| BseBI | CCWGG | 2 cut(s) | 93, 1602 |
| BseCI | ATCGAT | 1 cut(s) | 1593 |
| BseDI | CCNNGG | 3 cut(s) | 91, 590, 1970 |
| BseGI | GGATG | 6 cut(s) | 274, 1161, 1279, 1766, 1810, 1876 |
| BseJI | GATNNNNATC | 1 cut(s) | 492 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 64, 1016, 1719, 2143, 2144 |
| BseMI | GCAATG | 1 cut(s) | 1634 |
| BseMII | CTCAG | 3 cut(s) | 1442, 2196, 2315 |
| BseNI | ACTGG | 5 cut(s) | 527, 1258, 1399, 1615, 2315 |
| BseSI | GKGCMC | 2 cut(s) | 769, 1394 |
| BseXI | GCAGC | 1 cut(s) | 2061 |
| BseYI | CCCAGC | 2 cut(s) | 2228, 2279 |
| BsgI | GTGCAG | 1 cut(s) | 2289 |
| BshFI | GGCC | 2 cut(s) | 237, 1556 |
| BshNI | GGYRCC | 1 cut(s) | 1810 |
| BshVI | ATCGAT | 1 cut(s) | 1593 |
| BsiHKAI | GWGCWC | 2 cut(s) | 769, 2110 |
| BsiSI | CCGG | 2 cut(s) | 719, 1713 |
| BslFI | GGGAC | 2 cut(s) | 515, 2245 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 64, 1016, 1719, 2143, 2144 |
| BsmAI | GTCTC | 3 cut(s) | 543, 875, 1854 |
| BsmFI | GGGAC | 2 cut(s) | 515, 2245 |
| BsmI | GAATGC | 4 cut(s) | 1203, 1374, 1538, 1961 |
| BsnI | GGCC | 2 cut(s) | 237, 1556 |
| Bsp119I | TTCGAA | 1 cut(s) | 1261 |
| Bsp1286I | GDGCHC | 3 cut(s) | 769, 1394, 2110 |
| Bsp1407I | TGTACA | 1 cut(s) | 247 |
| Bsp143I | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| Bsp19I | CCATGG | 1 cut(s) | 1970 |
| BspACI | CCGC | 3 cut(s) | 307, 1487, 1557 |
| BspANI | GGCC | 2 cut(s) | 237, 1556 |
| BspCNI | CTCAG | 3 cut(s) | 1443, 2197, 2316 |
| BspDI | ATCGAT | 1 cut(s) | 1593 |
| BspHI | TCATGA | 2 cut(s) | 1075, 1290 |
| BspLI | GGNNCC | 4 cut(s) | 236, 397, 1812, 1968 |
| BspOI | GCTAGC | 1 cut(s) | 1643 |
| BspQI | GCTCTTC | 1 cut(s) | 336 |
| BspT104I | TTCGAA | 1 cut(s) | 1261 |
| BspT107I | GGYRCC | 1 cut(s) | 1810 |
| BsrDI | GCAATG | 1 cut(s) | 1634 |
| BsrGI | TGTACA | 1 cut(s) | 247 |
| BsrI | ACTGG | 5 cut(s) | 527, 1258, 1399, 1615, 2315 |
| BssECI | CCNNGG | 3 cut(s) | 91, 590, 1970 |
| BssMI | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| BssT1I | CCWWGG | 1 cut(s) | 1970 |
| Bst2UI | CCWGG | 2 cut(s) | 93, 1602 |
| Bst4CI | ACNGT | 8 cut(s) | 386, 411, 475, 606, 823, 886, 1884, 2223 |
| Bst6I | CTCTTC | 3 cut(s) | 187, 336, 1564 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2276 |
| BstAUI | TGTACA | 1 cut(s) | 247 |
| BstBAI | YACGTR | 2 cut(s) | 539, 1037 |
| BstBI | TTCGAA | 1 cut(s) | 1261 |
| BstC8I | GCNNGC | 2 cut(s) | 912, 1641 |
| BstDEI | CTNAG | 4 cut(s) | 1451, 1672, 2205, 2324 |
| BstDSI | CCRYGG | 2 cut(s) | 590, 1970 |
| BstF5I | GGATG | 6 cut(s) | 274, 1161, 1279, 1766, 1810, 1876 |
| BstKTI | GATC | 7 cut(s) | 112, 361, 545, 1070, 1360, 1408, 1849 |
| BstMAI | GTCTC | 3 cut(s) | 543, 875, 1854 |
| BstMBI | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 773, 2276 |
| BstNI | CCWGG | 2 cut(s) | 93, 1602 |
| BstNSI | RCATGY | 4 cut(s) | 248, 256, 914, 1832 |
| BstSCI | CCNGG | 3 cut(s) | 91, 717, 1600 |
| BstSLI | GKGCMC | 2 cut(s) | 769, 1394 |
| BstSNI | TACGTA | 1 cut(s) | 539 |
| BstV1I | GCAGC | 1 cut(s) | 2061 |
| BstV2I | GAAGAC | 6 cut(s) | 171, 405, 514, 897, 1470, 1622 |
| BstX2I | RGATCY | 2 cut(s) | 542, 1357 |
| BstYI | RGATCY | 2 cut(s) | 542, 1357 |
| Bsu15I | ATCGAT | 1 cut(s) | 1593 |
| BsuI | GTATCC | 1 cut(s) | 825 |
| BsuRI | GGCC | 2 cut(s) | 237, 1556 |
| BsuTUI | ATCGAT | 1 cut(s) | 1593 |
| BtgI | CCRYGG | 2 cut(s) | 590, 1970 |
| BtgZI | GCGATG | 1 cut(s) | 1923 |
| BtrI | CACGTC | 1 cut(s) | 51 |
| BtsCI | GGATG | 6 cut(s) | 274, 1161, 1279, 1766, 1810, 1876 |
| BtsIMutI | CAGTG | 2 cut(s) | 471, 1392 |
| Cac8I | GCNNGC | 2 cut(s) | 912, 1641 |
| CciI | TCATGA | 2 cut(s) | 1075, 1290 |
| Cfr13I | GGNCC | 5 cut(s) | 235, 386, 683, 1061, 1598 |
| ClaI | ATCGAT | 1 cut(s) | 1593 |
| Csp6I | GTAC | 7 cut(s) | 248, 1608, 1621, 1996, 2035, 2160, 2185 |
| CviQI | GTAC | 7 cut(s) | 248, 1608, 1621, 1996, 2035, 2160, 2185 |
| DdeI | CTNAG | 4 cut(s) | 1451, 1672, 2205, 2324 |
| DpnI | GATC | 7 cut(s) | 111, 360, 544, 1069, 1359, 1407, 1848 |
| DpnII | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| DraI | TTTAAA | 1 cut(s) | 793 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 516 |
| DriI | GACNNNNNGTC | 1 cut(s) | 743 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 516 |
| EaeI | YGGCCR | 1 cut(s) | 1554 |
| Eam1104I | CTCTTC | 3 cut(s) | 187, 336, 1564 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 743 |
| EarI | CTCTTC | 3 cut(s) | 187, 336, 1564 |
| Eco105I | TACGTA | 1 cut(s) | 539 |
| Eco130I | CCWWGG | 1 cut(s) | 1970 |
| Eco47I | GGWCC | 4 cut(s) | 386, 683, 1061, 1598 |
| Eco57I | CTGAAG | 1 cut(s) | 315 |
| EcoO109I | RGGNCCY | 2 cut(s) | 235, 683 |
| EcoRI | GAATTC | 1 cut(s) | 2253 |
| EcoRII | CCWGG | 2 cut(s) | 91, 1600 |
| EcoT14I | CCWWGG | 1 cut(s) | 1970 |
| EcoT22I | ATGCAT | 2 cut(s) | 493, 1961 |
| ErhI | CCWWGG | 1 cut(s) | 1970 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1607, 1639 |
| FaqI | GGGAC | 2 cut(s) | 515, 2245 |
| FauI | CCCGC | 1 cut(s) | 1494 |
| FauNDI | CATATG | 1 cut(s) | 375 |
| FblI | GTMKAC | 2 cut(s) | 675, 825 |
| Fnu4HI | GCNGC | 2 cut(s) | 1557, 2075 |
| FokI | GGATG | 6 cut(s) | 281, 1148, 1286, 1753, 1817, 1883 |
| Fsp4HI | GCNGC | 2 cut(s) | 1557, 2075 |
| GluI | GCNGC | 2 cut(s) | 1557, 2075 |
| GsaI | CCCAGC | 2 cut(s) | 2232, 2283 |
| GsuI | CTGGAG | 3 cut(s) | 567, 1416, 1623 |
| HaeIII | GGCC | 2 cut(s) | 237, 1556 |
| HapII | CCGG | 2 cut(s) | 719, 1713 |
| HincII | GTYRAC | 3 cut(s) | 826, 1247, 2091 |
| HindII | GTYRAC | 3 cut(s) | 826, 1247, 2091 |
| HindIII | AAGCTT | 1 cut(s) | 281 |
| HinfI | GANTC | 6 cut(s) | 379, 867, 1660, 1737, 1744, 2006 |
| HpaI | GTTAAC | 1 cut(s) | 2091 |
| HpaII | CCGG | 2 cut(s) | 719, 1713 |
| HphI | GGTGA | 4 cut(s) | 934, 970, 999, 1031 |
| Hpy166II | GTNNAC | 7 cut(s) | 250, 676, 767, 826, 1247, 1919, 2091 |
| Hpy188I | TCNGA | 8 cut(s) | 228, 715, 866, 1015, 1166, 1340, 2052, 2196 |
| Hpy8I | GTNNAC | 7 cut(s) | 250, 676, 767, 826, 1247, 1919, 2091 |
| Hpy99I | CGWCG | 1 cut(s) | 218 |
| HpyAV | CCTTC | 3 cut(s) | 960, 1010, 1052 |
| HpyCH4III | ACNGT | 8 cut(s) | 386, 411, 475, 606, 823, 886, 1884, 2223 |
| HpyCH4IV | ACGT | 6 cut(s) | 50, 213, 538, 1036, 1171, 1619 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 773, 2276 |
| HpyF3I | CTNAG | 4 cut(s) | 1451, 1672, 2205, 2324 |
| HpySE526I | ACGT | 6 cut(s) | 50, 213, 538, 1036, 1171, 1619 |
| KspAI | GTTAAC | 1 cut(s) | 2091 |
| Kzo9I | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| LguI | GCTCTTC | 1 cut(s) | 336 |
| LmnI | GCTCC | 2 cut(s) | 639, 2113 |
| Lsp1109I | GCAGC | 1 cut(s) | 2061 |
| LweI | GCATC | 7 cut(s) | 425, 478, 739, 1170, 1492, 1691, 2191 |
| MaeII | ACGT | 6 cut(s) | 50, 213, 538, 1036, 1171, 1619 |
| MaeIII | GTNAC | 3 cut(s) | 53, 614, 1225 |
| MalI | GATC | 7 cut(s) | 111, 360, 544, 1069, 1359, 1407, 1848 |
| MboI | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| MfeI | CAATTG | 1 cut(s) | 1506 |
| MflI | RGATCY | 2 cut(s) | 542, 1357 |
| MhlI | GDGCHC | 3 cut(s) | 769, 1394, 2110 |
| MlyI | GAGTC | 1 cut(s) | 2000 |
| MmeI | TCCRAC | 3 cut(s) | 246, 842, 1189 |
| Mph1103I | ATGCAT | 2 cut(s) | 493, 1961 |
| MroXI | GAANNNNTTC | 1 cut(s) | 894 |
| MslI | CAYNNNNRTG | 5 cut(s) | 557, 621, 1550, 1771, 1827 |
| MspI | CCGG | 2 cut(s) | 719, 1713 |
| MspR9I | CCNGG | 3 cut(s) | 93, 719, 1602 |
| MunI | CAATTG | 1 cut(s) | 1506 |
| Mva1269I | GAATGC | 4 cut(s) | 1203, 1374, 1538, 1961 |
| MvaI | CCWGG | 2 cut(s) | 93, 1602 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 773, 2276 |
| NciI | CCSGG | 1 cut(s) | 719 |
| NcoI | CCATGG | 1 cut(s) | 1970 |
| NdeI | CATATG | 1 cut(s) | 375 |
| NdeII | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| NheI | GCTAGC | 1 cut(s) | 1639 |
| NlaIV | GGNNCC | 4 cut(s) | 236, 397, 1812, 1968 |
| NmuCI | GTSAC | 2 cut(s) | 614, 1225 |
| NsiI | ATGCAT | 2 cut(s) | 493, 1961 |
| NspI | RCATGY | 4 cut(s) | 248, 256, 914, 1832 |
| NspV | TTCGAA | 1 cut(s) | 1261 |
| OliI | CACNNNNGTG | 1 cut(s) | 557 |
| PaeI | GCATGC | 1 cut(s) | 914 |
| PagI | TCATGA | 2 cut(s) | 1075, 1290 |
| PciI | ACATGT | 2 cut(s) | 244, 1828 |
| PciSI | GCTCTTC | 1 cut(s) | 336 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 56, 1855 |
| PctI | GAATGC | 4 cut(s) | 1203, 1374, 1538, 1961 |
| PdmI | GAANNNNTTC | 1 cut(s) | 894 |
| PfeI | GAWTC | 5 cut(s) | 379, 867, 1660, 1737, 1744 |
| PfoI | TCCNGGA | 1 cut(s) | 1600 |
| PkrI | GCNGC | 2 cut(s) | 1558, 2076 |
| PleI | GAGTC | 1 cut(s) | 2000 |
| PpsI | GAGTC | 1 cut(s) | 2000 |
| Ppu21I | YACGTR | 2 cut(s) | 539, 1037 |
| PpuMI | RGGWCCY | 1 cut(s) | 683 |
| PscI | ACATGT | 2 cut(s) | 244, 1828 |
| PshBI | ATTAAT | 1 cut(s) | 1092 |
| PsiI | TTATAA | 1 cut(s) | 585 |
| Psp1406I | AACGTT | 1 cut(s) | 1171 |
| Psp5II | RGGWCCY | 1 cut(s) | 683 |
| Psp6I | CCWGG | 2 cut(s) | 91, 1600 |
| PspFI | CCCAGC | 2 cut(s) | 2228, 2279 |
| PspGI | CCWGG | 2 cut(s) | 91, 1600 |
| PspN4I | GGNNCC | 4 cut(s) | 236, 397, 1812, 1968 |
| PspPI | GGNCC | 5 cut(s) | 235, 386, 683, 1061, 1598 |
| PspPPI | RGGWCCY | 1 cut(s) | 683 |
| PsuI | RGATCY | 2 cut(s) | 542, 1357 |
| RsaI | GTAC | 7 cut(s) | 249, 1609, 1622, 1997, 2036, 2161, 2186 |
| RsaNI | GTAC | 7 cut(s) | 248, 1608, 1621, 1996, 2035, 2160, 2185 |
| RseI | CAYNNNNRTG | 5 cut(s) | 557, 621, 1550, 1771, 1827 |
| SalI | GTCGAC | 1 cut(s) | 824 |
| SapI | GCTCTTC | 1 cut(s) | 336 |
| SatI | GCNGC | 2 cut(s) | 1557, 2075 |
| Sau3AI | GATC | 7 cut(s) | 109, 358, 542, 1067, 1357, 1405, 1846 |
| Sau96I | GGNCC | 5 cut(s) | 235, 386, 683, 1061, 1598 |
| ScaI | AGTACT | 1 cut(s) | 2161 |
| SchI | GAGTC | 1 cut(s) | 2000 |
| ScrFI | CCNGG | 3 cut(s) | 93, 719, 1602 |
| SduI | GDGCHC | 3 cut(s) | 769, 1394, 2110 |
| SfaNI | GCATC | 7 cut(s) | 425, 478, 739, 1170, 1492, 1691, 2191 |
| SfuI | TTCGAA | 1 cut(s) | 1261 |
| SinI | GGWCC | 4 cut(s) | 386, 683, 1061, 1598 |
| SmiI | ATTTAAAT | 1 cut(s) | 793 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 557, 621, 1550, 1771, 1827 |
| SmlI | CTYRAG | 2 cut(s) | 149, 2352 |
| SmoI | CTYRAG | 2 cut(s) | 149, 2352 |
| SnaBI | TACGTA | 1 cut(s) | 539 |
| SphI | GCATGC | 1 cut(s) | 914 |
| SsiI | CCGC | 3 cut(s) | 307, 1487, 1557 |
| SspI | AATATT | 2 cut(s) | 938, 2131 |
| StyD4I | CCNGG | 3 cut(s) | 91, 717, 1600 |
| StyI | CCWWGG | 1 cut(s) | 1970 |
| SwaI | ATTTAAAT | 1 cut(s) | 793 |
| TaaI | ACNGT | 8 cut(s) | 386, 411, 475, 606, 823, 886, 1884, 2223 |
| TaiI | ACGT | 6 cut(s) | 53, 216, 541, 1039, 1174, 1622 |
| TaqII | GACCGA | 1 cut(s) | 1078 |
| TatI | WGTACW | 3 cut(s) | 247, 1995, 2159 |
| TauI | GCSGC | 1 cut(s) | 1559 |
| TfiI | GAWTC | 5 cut(s) | 379, 867, 1660, 1737, 1744 |
| TscAI | CASTG | 2 cut(s) | 478, 1399 |
| TseFI | GTSAC | 2 cut(s) | 614, 1225 |
| TseI | GCWGC | 1 cut(s) | 2074 |
| Tsp45I | GTSAC | 2 cut(s) | 614, 1225 |
| TspRI | CASTG | 2 cut(s) | 478, 1399 |
| VneI | GTGCAC | 1 cut(s) | 765 |
| VpaK11BI | GGWCC | 4 cut(s) | 386, 683, 1061, 1598 |
| VspI | ATTAAT | 1 cut(s) | 1092 |
| XbaI | TCTAGA | 1 cut(s) | 1733 |
| XceI | RCATGY | 4 cut(s) | 248, 256, 914, 1832 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 184, 777 |
| XmiI | GTMKAC | 2 cut(s) | 675, 825 |
| XmnI | GAANNNNTTC | 1 cut(s) | 894 |
| ZrmI | AGTACT | 1 cut(s) | 2161 |
| Zsp2I | ATGCAT | 2 cut(s) | 493, 1961 |