Rw6G019620

Helicase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000080
Physical Location & Seq
Reverse (-)
39194132 .. 39198445
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G019620.1

Sequence Viewer

Length: 3609 bp
ATGTCTTATAATAGCGGGGTTGCGCAAGTATGTCGTAGACAGAGACGACGCAAACCTCATAATTGTGCCCGAAATTTTCATGAAAATATTGGAATAAAAAAGTATCATTTGCCACCACCAAAGACATGTCCGTATTGTCATGCCCGTCTGTTTCATCATGAAACCAAAGATATGTGTTGCTTAAATGGGAAAACTGTCATTCCTCCAATTCAATCTCCTCCAGAAATGGTTGAGTTATTTTTTGCTGAAACTCCACAAGGTGCCCATTTTAGACAAAATATTCGTGCTTATAACCATGTGTTTTCATTTACATCAATGGGTGTGCACATCGATCAAAGTTTAGCGACTGGTGGTAGTACTTTCACTTTTCGTGCTCAAGGATCCATATATCATAAGATAGGAAGTCTTCTACCAGATGAAGATAACAGACCAAGATTTCTACAATTGTATATATATGACACAGACCATGAAGTAGACAATCGTATGTTAGAGAATGAAGCATTGCATAGAGATGTGGTTGTAAACATACAACAAATCTTAAACCGACATAATCCATTTGTGCACGTATTTCATCATCTAGCTCGACGCCAAGATTTGGAAACTTGCAAACTCATCATTAGAGAGCAACCTGCAAACCAACTTCAATATAGTTTGCCCTCAGAGTCTCAGGTTGCTGCAATAATAGTGGGTGCAGATGATGTGGAAAATCTGAAAGGTCGTGATATAATGATTCGTCCACATGAGGAATCGATTTTACTTCGAGGAGGCCGCCTGTTGCAACAATATTGGCACAAGATCGTCCAGATTTATGAAGATGTTATTAACAAAGGAGTCTTAGGTAACATTATTGCATATGTTTATGTCATCGAATTTCAAAAACGTGGTCTCCCGCATGTTCATATGCTCCTTATGTTAAATGACGATGATAAACTGAATAACCCTGATGATTATGACAAAATTGTTCGAGCTGAGATACCAAGTCAAAATGAAGAGCCAAAGTTGCACAATGCAGTATTGAGGCACATGATACATGGTCCATGTGGAATACATAAGCGAAATGCCCCATGTATGAAGCGTGGAAGTTGCAAACGAGGTTATCCTAAGCCTTTTTCAACCAATACAATTCAAGGGAATGACTCTTATCCCATTTACCACAGGCGAGACATTCACTCAAATGCAGAAGTTGATAACAGTTGGGTTATTCCGTATAATCCTTGGCTTCTTGCAAAATATGATTGTCATATTAATGTTGAAATTTGTTGCAGCATTAAGAGCGTTAAGTATTTGTACAAATATGTATACAAAGGTCCAGATAAAGTAACATTTGAAGTTCGAAGAGAAGCCAATGAAGATGAAATAAGGAATTTTGTTGATGCGAGATGGGTCTGTGCACCAGAGGCTTTGTGGAAAATATTCAAATTCGTGATGAACCGAATGTATCCTTCCGTGGAACGATTGCAAATCCATCTTCCAGACAGACAAAATGTTTTTTTTAATGCATATGAAAGAGTAACAAATATCCTCGCTAATGATAGGAACTCGATGACAATGCTTACAGAGTTTTTTACAAAGAACAAGTACCATGAAAGTGCGCGTCAATACTTATATCGAGAATTTCCAGAAAGATTCAGATGGATTGACAACCAACGGACATGGGAAGAAAGGCAAAATAACCAAAAGGTAATTGGTAGAATATACACAGTATCCCCTTCAGAAGGAGAAAAGTTCTACTTACGTGTTCTTTTAAACCATGTTAGAGGACCTACATCATACGAGGATTTAAGGACAGTAGATGACATCTTGCAACCAACATTTAAGAAGGCAGTTGAAAAACGAGGTTTGTTGGAAGAGGATGATAGTATCCGACAGTGTTTAAATGAAGCATCAAACATACGTATGCCTTCATCTTTAAGAAGATTGTTTGCCACCATCTTGGTTTATTGTGAGCCTATTGGGGTACGAAGTCTATGGGATGAATTTTATAAATTCATGATAGAAGACTATACTTCCTCAAGCACCATAGACAATGCACATATCAGGAACAGACTTTTGCGAGACTTAAATGAATTGTTGGCGCAACATAATAAATCTATTTTTGATTATGATTTGCCGCAAATGACTGAAGATTACTGTGAAGATTCTACACTGCCTAGACTAATACAAGATGAAGTTTCAATTATCATTCCTCAACAAGACCGCAATGCAGTTCATCATCTGAATGAGGATCAGACATCTGCGTACAACACAATAATATCTGCTATTGAGTGTAAAAACAATGCCATATTTTTCATCGATGGTCCAGGAGGGACTGGAAAAACATACTTATATCGTGCATTATTAGCCACCTTGAGAAGCAATGATCATATTGTACTAGCAACAGCAACTTCTGGAATAGCGGCAACTATACTTCCTGGTGGGAGAACAGCACACTCAAGGTTTAAAATTCCACTCAATTCTGATGTATCTTCCACATGCTCCATTAGTAAACAATCCAGTTTAGCAAAATTGATACAGAGATCATCAGCAATTGTTTGTGACGAAGCACCAATGATGCATAAATATGCGTTTGAAGCTCTTGATCGAACTTTTAAAGACATAACAGGAGTTGATTTACCATTTGGAGGTAAAGTCATGATCTTCGGGGGAGATTTCCGACAAGTTCTGCCAGTTATCCATAAAGGTACAAGGTCAGAGATGATCCAGGCCAGTCTGATTAATGCTTCATTTTGGAAAAATGTAAAAATTCTTCGTTTGAGACAAAATATGAGATCCATCAATGATCCAGAGTTCTCAAAGTTTTTACTTAATGTTGGTAATGGAGAACAACCATATGTGACTGAAGACATGATAAAATTACCATCATGTATGGTGATACCATGGGAGGATGAACAGTCAATCAACCAATTAATTAGTGAAATTTTTCCAAATTTAGAATCTCATATAAATGACGCAACATACTTGGTTGAGAGGGCAATAATCACTCCAAAGAATGATGATGTTGATGTACTTAATGAGATGATTATAAGGCGATTTCCGGGGCCTGAACACATTATGTACTCTTTTGACTCAGTTGAAGATGACACCAGAAATATGTACCAACAAGAATTCTTAAATTCAATTTCACCTTCTGGTATGCCCCCACATCAATTAACTATAAAAAGAGGTGCCCCAATTATACTTTTGAGAAACATTGACCCAAAAATAGGATTATGCAATGGTACAAGATTGGCTTGCCGTGGATCATATAAGAACCTTATTGACGCAGAAATTTTAAATGGTCAGTTTGCCAAAACGAGAATATTTCTACCAAGAATCGCTCTGAAGAGTGCCAAGACTTCTGGACTTCCTTTTGAGATGACAAGGAAGCAATTCCCTGTAAAGTTAAGTTTCGCACTTACTATAAACAAATCACAAGGGCAAACAATACCAAATGTTGGCATCTACCTTCCAGATCATGTATTCAGTCACGGGCAACTATACGTAGCTTTATCACGAGGAATTTCAAGAGCTACCACAAAAGTGTTGGTAAAGAAAGGTTCATTAGAAGGGGAGGAAGGGGTGTTCACAAGAAATGTTGTGTATAAAGAGATTTTACTTCCTTCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helitron_like_N PIF1

Protein Analysis

1202

Amino Acids

138.47

Weight (kDa)

8.51

Isoelectric Point (pI)

54.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helitron_like_N PF14214 262 - 303 7.4e-12 Helitron helicase-like domain at N-terminus
AAA_30 PF13604 738 - 890 2.7e-06 AAA domain
PIF1 PF05970 739 - 943 1.2e-73 PIF1-like helicase
Pif1_2B_dom PF21530 1035 - 1076 5.9e-18 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G30739
fragaria_vesca FvH4_1g22680 FvH4_1g27610 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g06413 FvH4_2g09121 FvH4_3g12510 FvH4_3g14390 FvH4_3g14390 FvH4_3g22271 FvH4_3g22301 FvH4_3g27562 FvH4_3g32073 FvH4_3g32074 FvH4_3g36631 FvH4_3g36632 FvH4_4g22471 FvH4_4g27840 FvH4_4g34213 FvH4_5g07390 FvH4_5g19921 FvH4_5g24553 FvH4_5g25262 FvH4_5g25262 FvH4_5g25551 FvH4_5g25552 FvH4_5g26291 FvH4_5g30531 FvH4_5g33791 FvH4_6g00281 FvH4_6g14351 FvH4_6g29181 FvH4_6g38512 FvH4_6g38513 FvH4_7g14272 FvH4_7g16900 FvH4_7g16900 FvH4_7g16900
malus_domestica MD05G1321100.v1.1 MD11G1142100.v1.1 MD15G1301000.v1.1
prunus_persica Prupe.4G269900_v2.0.a1 Prupe.4G273400_v2.0.a1
pyrus_communis pycom01g00220 pycom01g00230 pycom01g00510 pycom01g12380 pycom04g01450 pycom11g26740 pycom12g05830 pycom15g02410 pycom15g39190 pycom16g21480 pycom5153684g00230
rosa_chinensis RchiOBHm_Chr0c36g0502891 RchiOBHm_Chr1g0343901 RchiOBHm_Chr1g0354911 RchiOBHm_Chr1g0354931 RchiOBHm_Chr2g0095281 RchiOBHm_Chr2g0130601 RchiOBHm_Chr2g0138981 RchiOBHm_Chr2g0140301 RchiOBHm_Chr3g0473521 RchiOBHm_Chr4g0421031 RchiOBHm_Chr4g0444461 RchiOBHm_Chr5g0006651 RchiOBHm_Chr5g0007701 RchiOBHm_Chr5g0010451 RchiOBHm_Chr5g0013161 RchiOBHm_Chr5g0039821 RchiOBHm_Chr5g0039831 RchiOBHm_Chr5g0051601 RchiOBHm_Chr5g0053341 RchiOBHm_Chr5g0066551 RchiOBHm_Chr5g0066561 RchiOBHm_Chr6g0270051 RchiOBHm_Chr7g0221811
rosa_multiflora Rmu_sc0000293.1_g000031 Rmu_sc0000315.1_g000010 Rmu_sc0000342.1_g000007 Rmu_sc0000368.1_g000031 Rmu_sc0000383.1_g000027 Rmu_sc0000383.1_g000028 Rmu_sc0000446.1_g000008 Rmu_sc0000642.1_g000009 Rmu_sc0000708.1_g000015 Rmu_sc0000733.1_g000005 Rmu_sc0000733.1_g000006 Rmu_sc0001074.1_g000029 Rmu_sc0001074.1_g000030 Rmu_sc0001083.1_g000002 Rmu_sc0001083.1_g000003 Rmu_sc0001400.1_g000026 Rmu_sc0001466.1_g000001 Rmu_sc0001590.1_g000012 Rmu_sc0001590.1_g000013 Rmu_sc0001593.1_g000007 Rmu_sc0001648.1_g000007 Rmu_sc0001648.1_g000008 Rmu_sc0001861.1_g000046 Rmu_sc0001861.1_g000047 Rmu_sc0002131.1_g000033 Rmu_sc0002153.1_g000001 Rmu_sc0002153.1_g000002 Rmu_sc0002284.1_g000017 Rmu_sc0002351.1_g000030 Rmu_sc0002495.1_g000040 Rmu_sc0002495.1_g000041 Rmu_sc0002540.1_g000006 Rmu_sc0002822.1_g000028 Rmu_sc0002822.1_g000029 Rmu_sc0002822.1_g000030 Rmu_sc0003074.1_g000028 Rmu_sc0003198.1_g000015 Rmu_sc0003207.1_g000042 Rmu_sc0003441.1_g000022 Rmu_sc0003880.1_g000015 Rmu_sc0004301.1_g000006 Rmu_sc0004368.1_g000014 Rmu_sc0004467.1_g000037 Rmu_sc0004467.1_g000038 Rmu_sc0004815.1_g000007 Rmu_sc0004836.1_g000008 Rmu_sc0005065.1_g000038 Rmu_sc0005400.1_g000007 Rmu_sc0005693.1_g000018 Rmu_sc0005956.1_g000001 Rmu_sc0005999.1_g000010 Rmu_sc0005999.1_g000011 Rmu_sc0006101.1_g000002 Rmu_sc0006101.1_g000003 Rmu_sc0006101.1_g000004 Rmu_sc0006119.1_g000014 Rmu_sc0006314.1_g000002 Rmu_sc0006478.1_g000019 Rmu_sc0006478.1_g000020 Rmu_sc0006729.1_g000003 Rmu_sc0006824.1_g000018 Rmu_sc0006898.1_g000009 Rmu_sc0006898.1_g000010 Rmu_sc0007131.1_g000012 Rmu_sc0007131.1_g000013 Rmu_sc0008829.1_g000003 Rmu_sc0008921.1_g000003 Rmu_sc0009472.1_g000019 Rmu_sc0009472.1_g000020 Rmu_sc0012301.1_g000003 Rmu_sc0015523.1_g000009 Rmu_sc0019288.1_g000001 Rmu_sc0019670.1_g000001 Rmu_sc0026849.1_g000001 Rmu_sc0026849.1_g000002 Rmu_sc0031895.1_g000001
rosa_roxburghii Rroxscaffold_176G00431570 Rroxscaffold_1G00011120 Rroxscaffold_2G00105630 Rroxscaffold_2G00122600 Rroxscaffold_2G00128220 Rroxscaffold_2G00129710 Rroxscaffold_3G00218940 Rroxscaffold_3G00247370 Rroxscaffold_4G00310060 Rroxscaffold_4G00313710 Rroxscaffold_4G00319150 Rroxscaffold_5G00346740 Rroxscaffold_5G00359690 Rroxscaffold_5G00365700 Rroxscaffold_6G00394170 Rroxscaffold_6G00404040 Rroxscaffold_7G00205320 Rroxscaffold_7G00211550 Rroxscaffold_7G00211560 Rroxscaffold_7G00215100
rosa_rugosa Rorug03G0285900 Rorug03G0286000 Rorug03G0286100 Rorug03G0286200 Rorug04G0098600 Rorug04G0098700 Rorug04G0098800 Rorug04G0139000 Rorug04G0209800 Rorug05G0101300 Rorug05G0101400 Rorug05G0101500 Rorug05G0415400 Rorug05G0466200 Rorug06G0017400 Rorug06G0017500 Rorug06G0151300 Rorug06G0318500 Rorug06G0318600 Rorug06G0318700.1
rosa_samantha Rh1CG085700 Rh1CG254600 Rh2BG228400 Rh2BG351000 Rh2CG225500 Rh2CG304800 Rh2DG104100 Rh2DG341000 Rh2DG380500 Rh3AG184300 Rh3AG184400 Rh3CG208500 Rh3CG208700 Rh3CG208900 Rh4AG207400 Rh4AG413200 Rh4BG424700 Rh4CG301500 Rh4CG439000 Rh4CG439100 Rh4CG439200 Rh5AG462900 Rh5BG193500 Rh5CG089900 Rh5CG300900 Rh5CG303800 Rh5CG343800 Rh5CG431500 Rh5CG431600 Rh5DG385700 Rh5DG396200 Rh6AG400600 Rh6AG400700 Rh6BG120400 Rh6BG293100 Rh6CG020800 Rh6DG504700 Rh7AG170200 Rh7AG364500 Rh7BG310700 Rh7BG326900 Rh7CG361900 Rh7DG342300
rosa_wichuraiana Rw0G001510 Rw0G002770 Rw0G002780 Rw0G008190 Rw0G012290 Rw0G013020 Rw0G019710 Rw0G022900 Rw1G005030 Rw1G006850 Rw1G007150 Rw1G008640 Rw1G010010 Rw1G014140 Rw1G014760 Rw1G021180 Rw1G024370 Rw2G008590 Rw2G008860 Rw2G013860 Rw2G018530 Rw2G019600 Rw2G021720 Rw2G022370 Rw3G005800 Rw3G024360 Rw3G027780 Rw3G028480 Rw3G029500 Rw3G029510 Rw4G000390 Rw4G008860 Rw4G013650 Rw4G016890 Rw4G016900 Rw4G018860 Rw4G018870 Rw4G032970 Rw5G004750 Rw5G004760 Rw5G004770 Rw5G011070 Rw5G011930 Rw5G015240 Rw5G015930 Rw5G019260 Rw5G021060 Rw5G027960 Rw5G028040 Rw5G028940 Rw5G031990 Rw5G044560 Rw5G049680 Rw6G014950 Rw6G015560 Rw6G016280 Rw6G017850 Rw6G018010 Rw6G019620 Rw6G028280 Rw7G010650 Rw7G016380 Rw7G020980 Rw7G025640 Rw7G027600 Rw7G029340 Rw7G035690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 9, 291, 1983, 3023
Acc16I TGCGCA 1 cut(s) 24
Acc36I ACCTGC 1 cut(s) 637
AccB1I GGYRCC 2 cut(s) 260, 3164
AccB7I CCANNNNNTGG 2 cut(s) 595, 3434
AccI GTMKAC 3 cut(s) 37, 474, 1299
AccII CGCG 1 cut(s) 1594
AciI CCGC 6 cut(s) 15, 769, 890, 2111, 2197, 2396
AclWI GGATC 7 cut(s) 375, 388, 2232, 2692, 2763, 2774, 3247
AcuI CTGAAG 4 cut(s) 1695, 2142, 2859, 3341
AcyI GRCGYC 1 cut(s) 586
AdeI CACNNNGTG 1 cut(s) 260
AfiI CCNNNNNNNGG 5 cut(s) 595, 1715, 2621, 3203, 3434
AflIII ACRYGT 2 cut(s) 125, 1735
AjnI CCWGG 3 cut(s) 2299, 2410, 2700
AleI CACNNNNGTG 1 cut(s) 3518
AloI GAACNNNNNNTCC 2 cut(s) 3545, 3577
AluBI AGCT 5 cut(s) 581, 968, 2573, 3485, 3509
AluI AGCT 5 cut(s) 581, 968, 2573, 3485, 3509
Alw21I GWGCWC 4 cut(s) 327, 376, 564, 1393
Alw26I GTCTC 6 cut(s) 37, 669, 890, 1155, 2049, 2749
Alw44I GTGCAC 3 cut(s) 323, 560, 1389
AlwI GGATC 7 cut(s) 375, 388, 2232, 2692, 2763, 2774, 3247
AoxI GGCC 3 cut(s) 766, 2703, 3038
ApaLI GTGCAC 3 cut(s) 323, 560, 1389
ApeKI GCWGC 2 cut(s) 674, 1263
ArsI GACNNNNNNTTYG 4 cut(s) 264, 296, 868, 900
AseI ATTAAT 3 cut(s) 1245, 2715, 2906
Asp700I GAANNNNTTC 3 cut(s) 1412, 2919, 3368
AspLEI GCGC 3 cut(s) 25, 1594, 2077
AspS9I GGNCC 5 cut(s) 1034, 1307, 1760, 2297, 3038
AsuC2I CCSGG 1 cut(s) 3036
AsuHPI GGTGA 2 cut(s) 2881, 3114
AsuII TTCGAA 1 cut(s) 1333
AvaII GGWCC 4 cut(s) 1034, 1307, 1760, 2297
BaeGI GKGCMC 6 cut(s) 70, 265, 327, 564, 1393, 3169
BamHI GGATCC 1 cut(s) 380
BanI GGYRCC 2 cut(s) 260, 3164
BarI GAAGNNNNNNTAC 2 cut(s) 2730, 2762
BauI CACGAG 1 cut(s) 3492
BbsI GAAGAC 3 cut(s) 398, 2004, 2847
Bbv12I GWGCWC 4 cut(s) 327, 376, 564, 1393
BbvI GCAGC 2 cut(s) 661, 1275
BccI CCATC 7 cut(s) 1374, 1473, 1626, 1937, 2288, 2780, 2866
BceAI ACGGC 1 cut(s) 3219
BcgI CGANNNNNNTGC 4 cut(s) 14, 48, 1531, 1565
BciT130I CCWGG 3 cut(s) 2301, 2412, 2702
BciVI GTATCC 3 cut(s) 1449, 1714, 1871
BclI TGATCA 1 cut(s) 2359
BcnI CCSGG 1 cut(s) 3036
BcoDI GTCTC 6 cut(s) 37, 669, 890, 1155, 2049, 2749
BfaI CTAG 3 cut(s) 578, 2151, 2372
BfuAI ACCTGC 1 cut(s) 637
BfuI GTATCC 3 cut(s) 1449, 1714, 1871
BisI GCNGC 5 cut(s) 675, 769, 1264, 2111, 2397
BlsI GCNGC 5 cut(s) 676, 770, 1265, 2112, 2398
BmcAI AGTACT 1 cut(s) 358
Bme1390I CCNGG 4 cut(s) 2301, 2412, 2702, 3036
Bme18I GGWCC 4 cut(s) 1034, 1307, 1760, 2297
BmgT120I GGNCC 5 cut(s) 1034, 1307, 1760, 2297, 3038
BmiI GGNNCC 4 cut(s) 262, 382, 3039, 3166
BmrFI CCNGG 4 cut(s) 2301, 2412, 2702, 3036
BmsI GCATC 4 cut(s) 1363, 1892, 2541, 3447
BpiI GAAGAC 3 cut(s) 398, 2004, 2847
BpmI CTGGAG 1 cut(s) 204
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 1333
BpuEI CTTGAG 4 cut(s) 360, 1996, 2368, 2416
BpuMI CCSGG 1 cut(s) 3036
Bsa29I ATCGAT 3 cut(s) 330, 749, 2292
BsaAI YACGTR 4 cut(s) 565, 1736, 1895, 3481
BsaHI GRCGYC 1 cut(s) 586
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 5 cut(s) 1214, 1446, 2876, 3035, 3235
BsaXI ACNNNNNCTCC 4 cut(s) 870, 900, 2613, 2643
Bsc4I CCNNNNNNNGG 5 cut(s) 595, 1715, 2621, 3203, 3434
Bse1I ACTGG 5 cut(s) 352, 2314, 2493, 2666, 2706
Bse3DI GCAATG 4 cut(s) 500, 2206, 2362, 3220
BseBI CCWGG 3 cut(s) 2301, 2412, 2702
BseCI ATCGAT 3 cut(s) 330, 749, 2292
BseDI CCNNGG 5 cut(s) 1214, 1446, 2876, 3035, 3235
BseGI GGATG 3 cut(s) 1858, 1978, 2890
BseLI CCNNNNNNNGG 5 cut(s) 595, 1715, 2621, 3203, 3434
BseMI GCAATG 4 cut(s) 500, 2206, 2362, 3220
BseMII CTCAG 4 cut(s) 672, 680, 960, 3081
BseNI ACTGG 5 cut(s) 352, 2314, 2493, 2666, 2706
BseRI GAGGAG 2 cut(s) 207, 777
BseSI GKGCMC 6 cut(s) 70, 265, 327, 564, 1393, 3169
BseXI GCAGC 2 cut(s) 661, 1275
BsgI GTGCAG 1 cut(s) 711
Bsh1236I CGCG 1 cut(s) 1594
BshFI GGCC 3 cut(s) 768, 2705, 3040
BshNI GGYRCC 2 cut(s) 260, 3164
BshVI ATCGAT 3 cut(s) 330, 749, 2292
BsiHKAI GWGCWC 4 cut(s) 327, 376, 564, 1393
BsiSI CCGG 1 cut(s) 3035
BslFI GGGAC 1 cut(s) 2320
BslI CCNNNNNNNGG 5 cut(s) 595, 1715, 2621, 3203, 3434
BsmAI GTCTC 6 cut(s) 37, 669, 890, 1155, 2049, 2749
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 2320
BsnI GGCC 3 cut(s) 768, 2705, 3040
Bso31I GGTCTC 1 cut(s) 890
Bsp119I TTCGAA 1 cut(s) 1333
Bsp1286I GDGCHC 7 cut(s) 70, 265, 327, 376, 564, 1393, 3169
Bsp1407I TGTACA 1 cut(s) 1287
Bsp19I CCATGG 1 cut(s) 2876
BspACI CCGC 6 cut(s) 15, 769, 890, 2111, 2197, 2396
BspANI GGCC 3 cut(s) 768, 2705, 3040
BspCNI CTCAG 4 cut(s) 671, 679, 961, 3080
BspDI ATCGAT 3 cut(s) 330, 749, 2292
BspFNI CGCG 1 cut(s) 1594
BspHI TCATGA 4 cut(s) 79, 157, 1989, 2631
BspLI GGNNCC 4 cut(s) 262, 382, 3039, 3166
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 7 cut(s) 375, 388, 2232, 2692, 2763, 2774, 3247
BspQI GCTCTTC 1 cut(s) 984
BspT104I TTCGAA 1 cut(s) 1333
BspT107I GGYRCC 2 cut(s) 260, 3164
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 4 cut(s) 500, 2206, 2362, 3220
BsrGI TGTACA 1 cut(s) 1287
BsrI ACTGG 5 cut(s) 352, 2314, 2493, 2666, 2706
BssECI CCNNGG 5 cut(s) 1214, 1446, 2876, 3035, 3235
BssNAI GTATAC 1 cut(s) 1300
BssNI GRCGYC 1 cut(s) 586
BssSI CACGAG 1 cut(s) 3492
BssT1I CCWWGG 2 cut(s) 1214, 2876
Bst1107I GTATAC 1 cut(s) 1300
Bst2BI CACGAG 1 cut(s) 3492
Bst2UI CCWGG 3 cut(s) 2301, 2412, 2702
Bst4CI ACNGT 7 cut(s) 196, 1193, 1702, 1789, 1869, 2132, 2892
Bst6I CTCTTC 4 cut(s) 984, 1330, 1842, 3317
BstACI GRCGYC 1 cut(s) 586
BstAUI TGTACA 1 cut(s) 1287
BstBAI YACGTR 4 cut(s) 565, 1736, 1895, 3481
BstBI TTCGAA 1 cut(s) 1333
BstC8I GCNNGC 1 cut(s) 3232
BstDEI CTNAG 6 cut(s) 658, 666, 835, 969, 1101, 3067
BstDSI CCRYGG 3 cut(s) 1446, 2876, 3235
BstENI CCTNNNNNAGG 1 cut(s) 1713
BstF5I GGATG 3 cut(s) 1858, 1978, 2890
BstFNI CGCG 1 cut(s) 1594
BstHHI GCGC 3 cut(s) 25, 1594, 2077
BstMAI GTCTC 6 cut(s) 37, 669, 890, 1155, 2049, 2749
BstMWI GCNNNNNNNGC 5 cut(s) 1000, 1272, 1397, 2339, 2570
BstNI CCWGG 3 cut(s) 2301, 2412, 2702
BstNSI RCATGY 3 cut(s) 129, 896, 2475
BstSCI CCNGG 4 cut(s) 2299, 2410, 2700, 3034
BstSLI GKGCMC 6 cut(s) 70, 265, 327, 564, 1393, 3169
BstSNI TACGTA 2 cut(s) 1895, 3481
BstUI CGCG 1 cut(s) 1594
BstV1I GCAGC 2 cut(s) 661, 1275
BstV2I GAAGAC 3 cut(s) 398, 2004, 2847
BstX2I RGATCY 2 cut(s) 380, 2768
BstXI CCANNNNNNTGG 1 cut(s) 1933
BstYI RGATCY 2 cut(s) 380, 2768
BstZ17I GTATAC 1 cut(s) 1300
Bsu15I ATCGAT 3 cut(s) 330, 749, 2292
BsuI GTATCC 3 cut(s) 1449, 1714, 1871
BsuRI GGCC 3 cut(s) 768, 2705, 3040
BsuTUI ATCGAT 3 cut(s) 330, 749, 2292
BtgI CCRYGG 3 cut(s) 1446, 2876, 3235
BtsCI GGATG 3 cut(s) 1858, 1978, 2890
BtsI GCAGTG 1 cut(s) 2144
BtsIMutI CAGTG 2 cut(s) 1874, 2144
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 1 cut(s) 3232
CciI TCATGA 4 cut(s) 79, 157, 1989, 2631
CfoI GCGC 3 cut(s) 25, 1594, 2077
Cfr13I GGNCC 5 cut(s) 1034, 1307, 1760, 2297, 3038
ClaI ATCGAT 3 cut(s) 330, 749, 2292
CseI GACGC 5 cut(s) 57, 594, 1583, 2957, 3269
CspCI CAANNNNNGTGG 2 cut(s) 666, 701
DdeI CTNAG 6 cut(s) 658, 666, 835, 969, 1101, 3067
DraI TTTAAA 5 cut(s) 1746, 1875, 2440, 2590, 3273
DraIII CACNNNGTG 1 cut(s) 260
Eam1104I CTCTTC 4 cut(s) 984, 1330, 1842, 3317
EarI CTCTTC 4 cut(s) 984, 1330, 1842, 3317
Eco105I TACGTA 2 cut(s) 1895, 3481
Eco130I CCWWGG 2 cut(s) 1214, 2876
Eco31I GGTCTC 1 cut(s) 890
Eco47I GGWCC 4 cut(s) 1034, 1307, 1760, 2297
Eco57I CTGAAG 4 cut(s) 1695, 2142, 2859, 3341
EcoNI CCTNNNNNAGG 1 cut(s) 1713
EcoO109I RGGNCCY 2 cut(s) 1760, 3038
EcoRI GAATTC 1 cut(s) 3104
EcoRII CCWGG 3 cut(s) 2299, 2410, 2700
EcoT14I CCWWGG 2 cut(s) 1214, 2876
EcoT22I ATGCAT 2 cut(s) 1501, 2556
ErhI CCWWGG 2 cut(s) 1214, 2876
Esp3I CGTCTC 1 cut(s) 37
FalI AAGNNNNNCTT 4 cut(s) 1715, 1747, 3214, 3246
FaqI GGGAC 1 cut(s) 2320
FauI CCCGC 2 cut(s) 8, 897
FauNDI CATATG 4 cut(s) 853, 900, 1501, 2830
FbaI TGATCA 1 cut(s) 2359
FblI GTMKAC 3 cut(s) 37, 474, 1299
Fnu4HI GCNGC 5 cut(s) 675, 769, 1264, 2111, 2397
FokI GGATG 3 cut(s) 1865, 1985, 2897
Fsp4HI GCNGC 5 cut(s) 675, 769, 1264, 2111, 2397
FspBI CTAG 3 cut(s) 578, 2151, 2372
FspI TGCGCA 1 cut(s) 24
GlaI GCGC 3 cut(s) 24, 1593, 2076
GluI GCNGC 5 cut(s) 675, 769, 1264, 2111, 2397
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 3 cut(s) 768, 2705, 3040
HapII CCGG 1 cut(s) 3035
HgaI GACGC 5 cut(s) 57, 594, 1583, 2957, 3269
HhaI GCGC 3 cut(s) 25, 1594, 2077
Hin1I GRCGYC 1 cut(s) 586
Hin6I GCGC 3 cut(s) 23, 1592, 2075
HinP1I GCGC 3 cut(s) 23, 1592, 2075
HpaII CCGG 1 cut(s) 3035
HphI GGTGA 2 cut(s) 2881, 3114
Hpy99I CGWCG 2 cut(s) 51, 588
HpyCH4III ACNGT 7 cut(s) 196, 1193, 1702, 1789, 1869, 2132, 2892
HpyCH4IV ACGT 5 cut(s) 564, 880, 1735, 1894, 3480
HpyF10VI GCNNNNNNNGC 5 cut(s) 1000, 1272, 1397, 2339, 2570
HpyF3I CTNAG 6 cut(s) 658, 666, 835, 969, 1101, 3067
HpySE526I ACGT 5 cut(s) 564, 880, 1735, 1894, 3480
Hsp92I GRCGYC 1 cut(s) 586
HspAI GCGC 3 cut(s) 23, 1592, 2075
Ksp22I TGATCA 1 cut(s) 2359
LguI GCTCTTC 1 cut(s) 984
LmnI GCTCC 2 cut(s) 909, 2480
Lsp1109I GCAGC 2 cut(s) 661, 1275
LweI GCATC 4 cut(s) 1363, 1892, 2541, 3447
MaeI CTAG 3 cut(s) 578, 2151, 2372
MaeII ACGT 5 cut(s) 564, 880, 1735, 1894, 3480
MaeIII GTNAC 6 cut(s) 839, 1318, 1510, 2534, 2833, 3464
MfeI CAATTG 2 cut(s) 443, 2526
MflI RGATCY 2 cut(s) 380, 2768
MhlI GDGCHC 7 cut(s) 70, 265, 327, 376, 564, 1393, 3169
MlyI GAGTC 4 cut(s) 671, 840, 1130, 3059
MmeI TCCRAC 3 cut(s) 1824, 1888, 2677
Mph1103I ATGCAT 2 cut(s) 1501, 2556
MroXI GAANNNNTTC 3 cut(s) 1412, 2919, 3368
MspI CCGG 1 cut(s) 3035
MspR9I CCNGG 4 cut(s) 2301, 2412, 2702, 3036
MunI CAATTG 2 cut(s) 443, 2526
MvaI CCWGG 3 cut(s) 2301, 2412, 2702
MvnI CGCG 1 cut(s) 1594
MwoI GCNNNNNNNGC 5 cut(s) 1000, 1272, 1397, 2339, 2570
NciI CCSGG 1 cut(s) 3036
NcoI CCATGG 1 cut(s) 2876
NdeI CATATG 4 cut(s) 853, 900, 1501, 2830
NlaIV GGNNCC 4 cut(s) 262, 382, 3039, 3166
NmuCI GTSAC 3 cut(s) 2534, 2833, 3464
NsbI TGCGCA 1 cut(s) 24
NsiI ATGCAT 2 cut(s) 1501, 2556
NspI RCATGY 3 cut(s) 129, 896, 2475
NspV TTCGAA 1 cut(s) 1333
OliI CACNNNNGTG 1 cut(s) 3518
PagI TCATGA 4 cut(s) 79, 157, 1989, 2631
PciI ACATGT 1 cut(s) 125
PciSI GCTCTTC 1 cut(s) 984
PdmI GAANNNNTTC 3 cut(s) 1412, 2919, 3368
PfeI GAWTC 6 cut(s) 730, 746, 1626, 2138, 2933, 3312
PflMI CCANNNNNTGG 2 cut(s) 595, 3434
PfoI TCCNGGA 1 cut(s) 2299
PkrI GCNGC 5 cut(s) 676, 770, 1265, 2112, 2398
PleI GAGTC 4 cut(s) 670, 839, 1130, 3059
PpsI GAGTC 4 cut(s) 670, 839, 1130, 3059
Ppu21I YACGTR 4 cut(s) 565, 1736, 1895, 3481
PpuMI RGGWCCY 1 cut(s) 1760
PscI ACATGT 1 cut(s) 125
PshBI ATTAAT 3 cut(s) 1245, 2715, 2906
PsiI TTATAA 4 cut(s) 9, 291, 1983, 3023
Psp5II RGGWCCY 1 cut(s) 1760
Psp6I CCWGG 3 cut(s) 2299, 2410, 2700
PspGI CCWGG 3 cut(s) 2299, 2410, 2700
PspN4I GGNNCC 4 cut(s) 262, 382, 3039, 3166
PspPI GGNCC 5 cut(s) 1034, 1307, 1760, 2297, 3038
PspPPI RGGWCCY 1 cut(s) 1760
PsuI RGATCY 2 cut(s) 380, 2768
SapI GCTCTTC 1 cut(s) 984
SatI GCNGC 5 cut(s) 675, 769, 1264, 2111, 2397
Sau96I GGNCC 5 cut(s) 1034, 1307, 1760, 2297, 3038
ScaI AGTACT 1 cut(s) 358
SchI GAGTC 4 cut(s) 671, 840, 1130, 3059
ScrFI CCNGG 4 cut(s) 2301, 2412, 2702, 3036
SduI GDGCHC 7 cut(s) 70, 265, 327, 376, 564, 1393, 3169
SfaNI GCATC 4 cut(s) 1363, 1892, 2541, 3447
SfuI TTCGAA 1 cut(s) 1333
SinI GGWCC 4 cut(s) 1034, 1307, 1760, 2297
SmlI CTYRAG 4 cut(s) 375, 2011, 2347, 2431
SmoI CTYRAG 4 cut(s) 375, 2011, 2347, 2431
SnaBI TACGTA 2 cut(s) 1895, 3481
SsiI CCGC 6 cut(s) 15, 769, 890, 2111, 2197, 2396
SspI AATATT 5 cut(s) 88, 280, 785, 1413, 3300
SspMI CTAG 3 cut(s) 578, 2151, 2372
StyD4I CCNGG 4 cut(s) 2299, 2410, 2700, 3034
StyI CCWWGG 2 cut(s) 1214, 2876
TaaI ACNGT 7 cut(s) 196, 1193, 1702, 1789, 1869, 2132, 2892
TaiI ACGT 5 cut(s) 567, 883, 1738, 1897, 3483
TatI WGTACW 5 cut(s) 356, 1287, 2368, 3004, 3054
TauI GCSGC 3 cut(s) 771, 2113, 2399
TfiI GAWTC 6 cut(s) 730, 746, 1626, 2138, 2933, 3312
TscAI CASTG 2 cut(s) 1874, 2151
TseFI GTSAC 3 cut(s) 2534, 2833, 3464
TseI GCWGC 2 cut(s) 674, 1263
Tsp45I GTSAC 3 cut(s) 2534, 2833, 3464
TspGWI ACGGA 4 cut(s) 120, 1194, 1435, 1663
TspRI CASTG 2 cut(s) 1874, 2151
Van91I CCANNNNNTGG 2 cut(s) 595, 3434
VneI GTGCAC 3 cut(s) 323, 560, 1389
VpaK11BI GGWCC 4 cut(s) 1034, 1307, 1760, 2297
VspI ATTAAT 3 cut(s) 1245, 2715, 2906
XagI CCTNNNNNAGG 1 cut(s) 1713
XceI RCATGY 3 cut(s) 129, 896, 2475
XcmI CCANNNNNNNNNTGG 3 cut(s) 1401, 1682, 3520
XmiI GTMKAC 3 cut(s) 37, 474, 1299
XmnI GAANNNNTTC 3 cut(s) 1412, 2919, 3368
XspI CTAG 3 cut(s) 578, 2151, 2372
ZrmI AGTACT 1 cut(s) 358
Zsp2I ATGCAT 2 cut(s) 1501, 2556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.