Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 296 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 911, 1333 |
| AccI | GTMKAC | 1 cut(s) | 1041 |
| AccII | CGCG | 9 cut(s) | 95, 183, 254, 287, 999, 1505, 1684, 1704, 1713 |
| AciI | CCGC | 5 cut(s) | 226, 909, 1682, 1702, 1713 |
| AclWI | GGATC | 2 cut(s) | 91, 1430 |
| AcoI | YGGCCR | 3 cut(s) | 3, 982, 1679 |
| AcsI | RAATTY | 5 cut(s) | 869, 1054, 1070, 1189, 1483 |
| AcyI | GRCGYC | 1 cut(s) | 1347 |
| AfaI | GTAC | 1 cut(s) | 564 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 404, 497, 911, 1333, 1389, 1542 |
| AflIII | ACRYGT | 1 cut(s) | 559 |
| AgsI | TTSAA | 9 cut(s) | 55, 392, 874, 880, 900, 1013, 1053, 1294, 1304 |
| AjnI | CCWGG | 4 cut(s) | 63, 149, 613, 1121 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 648, 680 |
| AluBI | AGCT | 6 cut(s) | 175, 312, 632, 1034, 1533, 1579 |
| AluI | AGCT | 6 cut(s) | 175, 312, 632, 1034, 1533, 1579 |
| Alw21I | GWGCWC | 1 cut(s) | 510 |
| Alw26I | GTCTC | 1 cut(s) | 556 |
| Alw44I | GTGCAC | 1 cut(s) | 506 |
| AlwI | GGATC | 2 cut(s) | 91, 1430 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 503, 1677 |
| AoxI | GGCC | 7 cut(s) | 3, 246, 902, 982, 1598, 1610, 1679 |
| ApaLI | GTGCAC | 1 cut(s) | 506 |
| ApeKI | GCWGC | 1 cut(s) | 1718 |
| ApoI | RAATTY | 5 cut(s) | 869, 1054, 1070, 1189, 1483 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 706, 718, 738, 750, 992, 1024 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 429, 884, 1073 |
| AspLEI | GCGC | 2 cut(s) | 297, 1713 |
| AspS9I | GGNCC | 2 cut(s) | 305, 734 |
| AsuHPI | GGTGA | 4 cut(s) | 491, 644, 806, 1208 |
| AvaII | GGWCC | 2 cut(s) | 305, 734 |
| BaeGI | GKGCMC | 1 cut(s) | 510 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 181, 214 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BauI | CACGAG | 1 cut(s) | 1475 |
| BbsI | GAAGAC | 1 cut(s) | 522 |
| Bbv12I | GWGCWC | 1 cut(s) | 510 |
| BccI | CCATC | 5 cut(s) | 86, 127, 129, 257, 1590 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1408, 1442 |
| BciT130I | CCWGG | 4 cut(s) | 65, 151, 615, 1123 |
| BciVI | GTATCC | 1 cut(s) | 211 |
| BclI | TGATCA | 3 cut(s) | 580, 604, 649 |
| BcoDI | GTCTC | 1 cut(s) | 556 |
| BfaI | CTAG | 3 cut(s) | 645, 839, 978 |
| BfmI | CTRYAG | 2 cut(s) | 399, 1042 |
| BfuI | GTATCC | 1 cut(s) | 211 |
| BisI | GCNGC | 3 cut(s) | 227, 1682, 1719 |
| BlsI | GCNGC | 3 cut(s) | 228, 1683, 1720 |
| Bme1390I | CCNGG | 4 cut(s) | 65, 151, 615, 1123 |
| Bme18I | GGWCC | 2 cut(s) | 305, 734 |
| BmgT120I | GGNCC | 2 cut(s) | 305, 734 |
| BmiI | GGNNCC | 3 cut(s) | 307, 1224, 1270 |
| BmrFI | CCNGG | 4 cut(s) | 65, 151, 615, 1123 |
| BmrI | ACTGGG | 1 cut(s) | 705 |
| BmsI | GCATC | 4 cut(s) | 600, 1108, 1558, 1580 |
| BmuI | ACTGGG | 1 cut(s) | 705 |
| BpiI | GAAGAC | 1 cut(s) | 522 |
| BpuEI | CTTGAG | 1 cut(s) | 306 |
| BsaHI | GRCGYC | 1 cut(s) | 1347 |
| BsaJI | CCNNGG | 2 cut(s) | 150, 614 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 404, 497, 911, 1333, 1389, 1542 |
| Bse1I | ACTGG | 2 cut(s) | 700, 1682 |
| BseBI | CCWGG | 4 cut(s) | 65, 151, 615, 1123 |
| BseDI | CCNNGG | 2 cut(s) | 150, 614 |
| BseGI | GGATG | 1 cut(s) | 779 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 404, 497, 911, 1333, 1389, 1542 |
| BseMII | CTCAG | 2 cut(s) | 204, 375 |
| BseNI | ACTGG | 2 cut(s) | 700, 1682 |
| BseRI | GAGGAG | 1 cut(s) | 1187 |
| BseSI | GKGCMC | 1 cut(s) | 510 |
| BseYI | CCCAGC | 1 cut(s) | 308 |
| Bsh1236I | CGCG | 9 cut(s) | 95, 183, 254, 287, 999, 1505, 1684, 1704, 1713 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1702 |
| BshFI | GGCC | 7 cut(s) | 5, 248, 904, 984, 1600, 1612, 1681 |
| BsiEI | CGRYCG | 1 cut(s) | 1702 |
| BsiHKAI | GWGCWC | 1 cut(s) | 510 |
| BslFI | GGGAC | 3 cut(s) | 291, 633, 1405 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 404, 497, 911, 1333, 1389, 1542 |
| BsmAI | GTCTC | 1 cut(s) | 556 |
| BsmFI | GGGAC | 3 cut(s) | 291, 633, 1405 |
| BsnI | GGCC | 7 cut(s) | 5, 248, 904, 984, 1600, 1612, 1681 |
| Bsp1286I | GDGCHC | 1 cut(s) | 510 |
| Bsp143I | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| Bsp68I | TCGCGA | 4 cut(s) | 95, 254, 287, 999 |
| BspACI | CCGC | 5 cut(s) | 226, 909, 1682, 1702, 1713 |
| BspANI | GGCC | 7 cut(s) | 5, 248, 904, 984, 1600, 1612, 1681 |
| BspCNI | CTCAG | 2 cut(s) | 205, 376 |
| BspFNI | CGCG | 9 cut(s) | 95, 183, 254, 287, 999, 1505, 1684, 1704, 1713 |
| BspLI | GGNNCC | 3 cut(s) | 307, 1224, 1270 |
| BspPI | GGATC | 2 cut(s) | 91, 1430 |
| BspQI | GCTCTTC | 1 cut(s) | 1701 |
| BsrI | ACTGG | 2 cut(s) | 700, 1682 |
| BssECI | CCNNGG | 2 cut(s) | 150, 614 |
| BssMI | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| BssNI | GRCGYC | 1 cut(s) | 1347 |
| BssSI | CACGAG | 1 cut(s) | 1475 |
| Bst2BI | CACGAG | 1 cut(s) | 1475 |
| Bst2UI | CCWGG | 4 cut(s) | 65, 151, 615, 1123 |
| Bst4CI | ACNGT | 5 cut(s) | 521, 1004, 1046, 1095, 1290 |
| Bst6I | CTCTTC | 2 cut(s) | 33, 1701 |
| BstACI | GRCGYC | 1 cut(s) | 1347 |
| BstC8I | GCNNGC | 6 cut(s) | 177, 181, 367, 474, 1686, 1723 |
| BstDEI | CTNAG | 3 cut(s) | 213, 384, 417 |
| BstEII | GGTNACC | 1 cut(s) | 794 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 402, 495, 1540 |
| BstF5I | GGATG | 1 cut(s) | 779 |
| BstFNI | CGCG | 9 cut(s) | 95, 183, 254, 287, 999, 1505, 1684, 1704, 1713 |
| BstHHI | GCGC | 2 cut(s) | 297, 1713 |
| BstKTI | GATC | 9 cut(s) | 99, 412, 583, 607, 652, 661, 1179, 1387, 1425 |
| BstMAI | GTCTC | 1 cut(s) | 556 |
| BstMBI | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| BstMCI | CGRYCG | 1 cut(s) | 1702 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 11, 146, 232, 960, 1435, 1597, 1710 |
| BstNI | CCWGG | 4 cut(s) | 65, 151, 615, 1123 |
| BstNSI | RCATGY | 1 cut(s) | 563 |
| BstPI | GGTNACC | 1 cut(s) | 794 |
| BstSCI | CCNGG | 4 cut(s) | 63, 149, 613, 1121 |
| BstSFI | CTRYAG | 2 cut(s) | 399, 1042 |
| BstSLI | GKGCMC | 1 cut(s) | 510 |
| BstUI | CGCG | 9 cut(s) | 95, 183, 254, 287, 999, 1505, 1684, 1704, 1713 |
| BstV2I | GAAGAC | 1 cut(s) | 522 |
| BstX2I | RGATCY | 1 cut(s) | 1422 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 595 |
| BstYI | RGATCY | 1 cut(s) | 1422 |
| BsuI | GTATCC | 1 cut(s) | 211 |
| BsuRI | GGCC | 7 cut(s) | 5, 248, 904, 984, 1600, 1612, 1681 |
| BtgZI | GCGATG | 2 cut(s) | 107, 235 |
| BtsCI | GGATG | 1 cut(s) | 779 |
| BtsIMutI | CAGTG | 1 cut(s) | 1051 |
| BtuMI | TCGCGA | 4 cut(s) | 95, 254, 287, 999 |
| Cac8I | GCNNGC | 6 cut(s) | 177, 181, 367, 474, 1686, 1723 |
| CaiI | CAGNNNCTG | 2 cut(s) | 503, 1677 |
| CfoI | GCGC | 2 cut(s) | 297, 1713 |
| Cfr13I | GGNCC | 2 cut(s) | 305, 734 |
| CseI | GACGC | 2 cut(s) | 1355, 1511 |
| Csp6I | GTAC | 1 cut(s) | 563 |
| CviQI | GTAC | 1 cut(s) | 563 |
| DdeI | CTNAG | 3 cut(s) | 213, 384, 417 |
| DpnI | GATC | 9 cut(s) | 98, 411, 582, 606, 651, 660, 1178, 1386, 1424 |
| DpnII | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| EaeI | YGGCCR | 3 cut(s) | 3, 982, 1679 |
| Eam1104I | CTCTTC | 2 cut(s) | 33, 1701 |
| EarI | CTCTTC | 2 cut(s) | 33, 1701 |
| Eco47I | GGWCC | 2 cut(s) | 305, 734 |
| Eco91I | GGTNACC | 1 cut(s) | 794 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 402, 495, 1540 |
| EcoO109I | RGGNCCY | 1 cut(s) | 305 |
| EcoO65I | GGTNACC | 1 cut(s) | 794 |
| EcoRII | CCWGG | 4 cut(s) | 63, 149, 613, 1121 |
| EcoT22I | ATGCAT | 1 cut(s) | 1573 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 648, 680, 1371, 1403 |
| FaqI | GGGAC | 3 cut(s) | 291, 633, 1405 |
| FauI | CCCGC | 1 cut(s) | 1706 |
| FauNDI | CATATG | 1 cut(s) | 1281 |
| FbaI | TGATCA | 3 cut(s) | 580, 604, 649 |
| FblI | GTMKAC | 1 cut(s) | 1041 |
| Fnu4HI | GCNGC | 3 cut(s) | 227, 1682, 1719 |
| FokI | GGATG | 1 cut(s) | 786 |
| Fsp4HI | GCNGC | 3 cut(s) | 227, 1682, 1719 |
| FspBI | CTAG | 3 cut(s) | 645, 839, 978 |
| FspI | TGCGCA | 1 cut(s) | 296 |
| GlaI | GCGC | 2 cut(s) | 296, 1712 |
| GluI | GCNGC | 3 cut(s) | 227, 1682, 1719 |
| GsaI | CCCAGC | 1 cut(s) | 312 |
| HaeIII | GGCC | 7 cut(s) | 5, 248, 904, 984, 1600, 1612, 1681 |
| HgaI | GACGC | 2 cut(s) | 1355, 1511 |
| HhaI | GCGC | 2 cut(s) | 297, 1713 |
| Hin1I | GRCGYC | 1 cut(s) | 1347 |
| Hin6I | GCGC | 2 cut(s) | 295, 1711 |
| HinP1I | GCGC | 2 cut(s) | 295, 1711 |
| HinfI | GANTC | 6 cut(s) | 169, 352, 482, 893, 1049, 1199 |
| HphI | GGTGA | 4 cut(s) | 491, 644, 806, 1208 |
| Hpy166II | GTNNAC | 3 cut(s) | 332, 508, 1042 |
| Hpy188I | TCNGA | 4 cut(s) | 280, 556, 609, 1576 |
| Hpy188III | TCNNGA | 6 cut(s) | 94, 253, 286, 356, 437, 998 |
| Hpy8I | GTNNAC | 3 cut(s) | 332, 508, 1042 |
| Hpy99I | CGWCG | 1 cut(s) | 1505 |
| HpyAV | CCTTC | 5 cut(s) | 62, 157, 296, 365, 1480 |
| HpyCH4III | ACNGT | 5 cut(s) | 521, 1004, 1046, 1095, 1290 |
| HpyCH4IV | ACGT | 3 cut(s) | 627, 636, 1218 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 11, 146, 232, 960, 1435, 1597, 1710 |
| HpyF3I | CTNAG | 3 cut(s) | 213, 384, 417 |
| HpySE526I | ACGT | 3 cut(s) | 627, 636, 1218 |
| Hsp92I | GRCGYC | 1 cut(s) | 1347 |
| HspAI | GCGC | 2 cut(s) | 295, 1711 |
| Ksp22I | TGATCA | 3 cut(s) | 580, 604, 649 |
| Kzo9I | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| LguI | GCTCTTC | 1 cut(s) | 1701 |
| LmnI | GCTCC | 4 cut(s) | 71, 1222, 1538, 1576 |
| LweI | GCATC | 4 cut(s) | 600, 1108, 1558, 1580 |
| MaeI | CTAG | 3 cut(s) | 645, 839, 978 |
| MaeII | ACGT | 3 cut(s) | 627, 636, 1218 |
| MaeIII | GTNAC | 1 cut(s) | 794 |
| MalI | GATC | 9 cut(s) | 98, 411, 582, 606, 651, 660, 1178, 1386, 1424 |
| MboI | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| MflI | RGATCY | 1 cut(s) | 1422 |
| MhlI | GDGCHC | 1 cut(s) | 510 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 1 cut(s) | 361 |
| MmeI | TCCRAC | 1 cut(s) | 327 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| Mph1103I | ATGCAT | 1 cut(s) | 1573 |
| MroXI | GAANNNNTTC | 3 cut(s) | 429, 884, 1073 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MseI | TTAA | 5 cut(s) | 677, 816, 827, 1259, 1314 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspR9I | CCNGG | 4 cut(s) | 65, 151, 615, 1123 |
| MvaI | CCWGG | 4 cut(s) | 65, 151, 615, 1123 |
| MvnI | CGCG | 9 cut(s) | 95, 183, 254, 287, 999, 1505, 1684, 1704, 1713 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 11, 146, 232, 960, 1435, 1597, 1710 |
| NdeI | CATATG | 1 cut(s) | 1281 |
| NdeII | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| NlaIV | GGNNCC | 3 cut(s) | 307, 1224, 1270 |
| NmuCI | GTSAC | 1 cut(s) | 794 |
| NruI | TCGCGA | 4 cut(s) | 95, 254, 287, 999 |
| NsbI | TGCGCA | 1 cut(s) | 296 |
| NsiI | ATGCAT | 1 cut(s) | 1573 |
| NspI | RCATGY | 1 cut(s) | 563 |
| PciI | ACATGT | 1 cut(s) | 559 |
| PciSI | GCTCTTC | 1 cut(s) | 1701 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 633 |
| PdmI | GAANNNNTTC | 3 cut(s) | 429, 884, 1073 |
| PfeI | GAWTC | 5 cut(s) | 169, 482, 893, 1049, 1199 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 911, 1333 |
| PfoI | TCCNGGA | 1 cut(s) | 63 |
| PkrI | GCNGC | 3 cut(s) | 228, 1683, 1720 |
| PleI | GAGTC | 1 cut(s) | 360 |
| PpsI | GAGTC | 1 cut(s) | 360 |
| PpuMI | RGGWCCY | 1 cut(s) | 305 |
| PscI | ACATGT | 1 cut(s) | 559 |
| Psp5II | RGGWCCY | 1 cut(s) | 305 |
| Psp6I | CCWGG | 4 cut(s) | 63, 149, 613, 1121 |
| PspEI | GGTNACC | 1 cut(s) | 794 |
| PspFI | CCCAGC | 1 cut(s) | 308 |
| PspGI | CCWGG | 4 cut(s) | 63, 149, 613, 1121 |
| PspN4I | GGNNCC | 3 cut(s) | 307, 1224, 1270 |
| PspPI | GGNCC | 2 cut(s) | 305, 734 |
| PspPPI | RGGWCCY | 1 cut(s) | 305 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 621, 653 |
| PstNI | CAGNNNCTG | 2 cut(s) | 503, 1677 |
| PsuI | RGATCY | 1 cut(s) | 1422 |
| RruI | TCGCGA | 4 cut(s) | 95, 254, 287, 999 |
| RsaI | GTAC | 1 cut(s) | 564 |
| RsaNI | GTAC | 1 cut(s) | 563 |
| SapI | GCTCTTC | 1 cut(s) | 1701 |
| SaqAI | TTAA | 5 cut(s) | 677, 816, 827, 1259, 1314 |
| SatI | GCNGC | 3 cut(s) | 227, 1682, 1719 |
| Sau3AI | GATC | 9 cut(s) | 96, 409, 580, 604, 649, 658, 1176, 1384, 1422 |
| Sau96I | GGNCC | 2 cut(s) | 305, 734 |
| SchI | GAGTC | 1 cut(s) | 361 |
| ScrFI | CCNGG | 4 cut(s) | 65, 151, 615, 1123 |
| SduI | GDGCHC | 1 cut(s) | 510 |
| SfaNI | GCATC | 4 cut(s) | 600, 1108, 1558, 1580 |
| SfcI | CTRYAG | 2 cut(s) | 399, 1042 |
| SinI | GGWCC | 2 cut(s) | 305, 734 |
| SmlI | CTYRAG | 1 cut(s) | 321 |
| SmoI | CTYRAG | 1 cut(s) | 321 |
| SsiI | CCGC | 5 cut(s) | 226, 909, 1682, 1702, 1713 |
| SspI | AATATT | 2 cut(s) | 1149, 1463 |
| SspMI | CTAG | 3 cut(s) | 645, 839, 978 |
| StyD4I | CCNGG | 4 cut(s) | 63, 149, 613, 1121 |
| TaaI | ACNGT | 5 cut(s) | 521, 1004, 1046, 1095, 1290 |
| TaiI | ACGT | 3 cut(s) | 630, 639, 1221 |
| TaqI | TCGA | 6 cut(s) | 81, 114, 438, 567, 1418, 1500 |
| TatI | WGTACW | 1 cut(s) | 562 |
| TauI | GCSGC | 2 cut(s) | 229, 1684 |
| TfiI | GAWTC | 5 cut(s) | 169, 482, 893, 1049, 1199 |
| Tru1I | TTAA | 5 cut(s) | 677, 816, 827, 1259, 1314 |
| Tru9I | TTAA | 5 cut(s) | 677, 816, 827, 1259, 1314 |
| TscAI | CASTG | 1 cut(s) | 1051 |
| TseFI | GTSAC | 1 cut(s) | 794 |
| TseI | GCWGC | 1 cut(s) | 1718 |
| Tsp45I | GTSAC | 1 cut(s) | 794 |
| TspDTI | ATGAA | 6 cut(s) | 422, 656, 692, 877, 1173, 1251 |
| TspGWI | ACGGA | 2 cut(s) | 525, 1087 |
| TspRI | CASTG | 1 cut(s) | 1051 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 911, 1333 |
| VneI | GTGCAC | 1 cut(s) | 506 |
| VpaK11BI | GGWCC | 2 cut(s) | 305, 734 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 402, 495, 1540 |
| XapI | RAATTY | 5 cut(s) | 869, 1054, 1070, 1189, 1483 |
| XceI | RCATGY | 1 cut(s) | 563 |
| XmiI | GTMKAC | 1 cut(s) | 1041 |
| XmnI | GAANNNNTTC | 3 cut(s) | 429, 884, 1073 |
| XspI | CTAG | 3 cut(s) | 645, 839, 978 |
| Zsp2I | ATGCAT | 1 cut(s) | 1573 |