Rroxscaffold_4G00322220

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000314
Physical Location & Seq
Forward (+)
52378830 .. 52380983
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00322220.1

Sequence Viewer

Length: 579 bp
ATGACGGCTTCGGTGCTCTCCTCCGGCTACGATCCGTGGCTCGCGCATTCGTATGAACCGTGGTTAAATGAACATGCGGACTTGAACCGCATTTTCCTCATAGGGGACAGTTCGGGAGGAAACATTGTCCATGAAGTGGCTGCTCGGGCGGGGAATGCGGATTTGGGTCGATTCAAGTTATCCGGTGGGATTCTTATCCAACTCCGGATTTGTCCGGGTAACGAGGAGCAAGTCGGAGTTGGAACAGCCGAGAGACATCATTTTTTAACGGAGAATATCGAGAACCATAACAAGAAGCCGAATGCGGATCTCGCCGAGAGACCGCAAAGTCGCAAGGCTCCTCCGCGAGTTCGAGCAGCGTATGACTCGCTATGCGGACGATACCGGGCAACGGGTCACACAGATAGAGGGCGCCTTGAAGGCACAAGCCGTAGTTATCGCCGCTGCACAAAACGAGCATCATACGACAATTCGAATGCTATAGCGGATCATACCGGGCAAACGACATCAAACATAGCAGATCTCCGCAAGGATGGGGCCAACTTGGCAACCGAAGTGGCTATGCGAGAGCCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

192

Amino Acids

21.12

Weight (kDa)

8.48

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 19 - 76 8.6e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000314)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02272 FvH4_1g22661 FvH4_2g10242 FvH4_2g34051 FvH4_3g19221 FvH4_3g29651 FvH4_3g30891 FvH4_4g11061 FvH4_5g09250 FvH4_5g15302 FvH4_5g15303 FvH4_5g15304 FvH4_5g28521 FvH4_6g23591 FvH4_7g05710 FvH4_7g19520
pyrus_communis pycom07g06330
rosa_chinensis RchiOBHm_Chr5g0027301 RchiOBHm_Chr6g0251451 RchiOBHm_Chr6g0274861
rosa_laevigata RLG00000007850
rosa_multiflora Rmu_co8230721.1_g000001 Rmu_sc0000271.1_g000027 Rmu_sc0000303.1_g000024 Rmu_sc0000368.1_g000024 Rmu_sc0000621.1_g000037 Rmu_sc0000693.1_g000081 Rmu_sc0001229.1_g000006 Rmu_sc0001373.1_g000003 Rmu_sc0001459.1_g000001 Rmu_sc0001744.1_g000002 Rmu_sc0001909.1_g000002 Rmu_sc0002053.1_g000012 Rmu_sc0002060.1_g000015 Rmu_sc0002119.1_g000036 Rmu_sc0002177.1_g000007 Rmu_sc0002822.1_g000024 Rmu_sc0002965.1_g000015 Rmu_sc0003363.1_g000051 Rmu_sc0003500.1_g000013 Rmu_sc0004191.1_g000020 Rmu_sc0004904.1_g000032 Rmu_sc0005325.1_g000008 Rmu_sc0005947.1_g000040 Rmu_sc0006475.1_g000001 Rmu_sc0006522.1_g000019 Rmu_sc0007102.1_g000005 Rmu_sc0007810.1_g000003 Rmu_sc0008339.1_g000025 Rmu_sc0008698.1_g000004 Rmu_sc0009313.1_g000006 Rmu_sc0009850.1_g000006 Rmu_sc0010598.1_g000003 Rmu_sc0011162.1_g000002 Rmu_sc0011824.1_g000004 Rmu_sc0011900.1_g000010 Rmu_sc0012291.1_g000006 Rmu_sc0012619.1_g000003 Rmu_sc0014679.1_g000001 Rmu_sc0019053.1_g000001 Rmu_sc0021462.1_g000003 Rmu_sc0024172.1_g000001 Rmu_sc0024173.1_g000001 Rmu_ssc0000052.1_g000016 Rmu_ssc0000392.1_g000010 Rmu_ssc0000421.1_g000062 Rmu_ssc0000479.1_g000006
rosa_roxburghii Rroxscaffold_153G00436600 Rroxscaffold_1G00008580 Rroxscaffold_1G00016770 Rroxscaffold_1G00029550 Rroxscaffold_1G00036450 Rroxscaffold_1G00040410 Rroxscaffold_1G00047110 Rroxscaffold_1G00060570 Rroxscaffold_2G00081500 Rroxscaffold_2G00081510 Rroxscaffold_2G00086080 Rroxscaffold_2G00099620 Rroxscaffold_2G00099630 Rroxscaffold_2G00099640 Rroxscaffold_2G00109170 Rroxscaffold_2G00127750 Rroxscaffold_2G00128590 Rroxscaffold_2G00130780 Rroxscaffold_2G00131040 Rroxscaffold_2G00143160 Rroxscaffold_3G00229280 Rroxscaffold_3G00229290 Rroxscaffold_3G00229970 Rroxscaffold_3G00242860 Rroxscaffold_3G00242890 Rroxscaffold_3G00242900 Rroxscaffold_3G00248200 Rroxscaffold_4G00284550 Rroxscaffold_4G00288780 Rroxscaffold_4G00298620 Rroxscaffold_4G00298630 Rroxscaffold_4G00312420 Rroxscaffold_4G00320310 Rroxscaffold_4G00322220 Rroxscaffold_4G00323600 Rroxscaffold_4G00331830 Rroxscaffold_4G00332010 Rroxscaffold_4G00332020 Rroxscaffold_5G00344470 Rroxscaffold_5G00345310 Rroxscaffold_5G00356120 Rroxscaffold_5G00368700 Rroxscaffold_5G00379820 Rroxscaffold_5G00382480 Rroxscaffold_6G00395080 Rroxscaffold_6G00405520 Rroxscaffold_6G00428210 Rroxscaffold_7G00193460
rosa_samantha Rh7CG413200 Rh7DG284900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 411
AccB7I CCANNNNNTGG 1 cut(s) 136
AccII CGCG 2 cut(s) 44, 346
AccIII TCCGGA 1 cut(s) 204
AclWI GGATC 3 cut(s) 26, 315, 495
AcyI GRCGYC 1 cut(s) 412
AfiI CCNNNNNNNGG 3 cut(s) 103, 136, 391
AgsI TTSAA 3 cut(s) 85, 175, 419
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 2 cut(s) 247, 313
AlwI GGATC 3 cut(s) 26, 315, 495
Ama87I CYCGRG 1 cut(s) 144
Aor13HI TCCGGA 1 cut(s) 204
AoxI GGCC 1 cut(s) 537
ApeKI GCWGC 3 cut(s) 140, 356, 444
AspLEI GCGC 2 cut(s) 46, 414
AspS9I GGNCC 1 cut(s) 537
AsuC2I CCSGG 3 cut(s) 216, 386, 496
AsuII TTCGAA 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 144
BanI GGYRCC 1 cut(s) 411
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 127, 368, 431
BccI CCATC 1 cut(s) 527
BceAI ACGGC 2 cut(s) 21, 414
BcnI CCSGG 3 cut(s) 216, 386, 496
BcoDI GTCTC 2 cut(s) 247, 313
BfmI CTRYAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 415
BglI GCCNNNNNGGC 2 cut(s) 420, 545
BglII AGATCT 1 cut(s) 520
BisI GCNGC 4 cut(s) 141, 357, 442, 445
BlsI GCNGC 4 cut(s) 142, 358, 443, 446
Bme1390I CCNGG 3 cut(s) 216, 386, 496
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 3 cut(s) 339, 413, 538
BmrFI CCNGG 3 cut(s) 216, 386, 496
BmsI GCATC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 473
BpuMI CCSGG 3 cut(s) 216, 386, 496
BsaBI GATNNNNATC 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 412
BsaI GGTCTC 1 cut(s) 313
BsaJI CCNNGG 2 cut(s) 35, 59
BsaWI WCCGGW 2 cut(s) 182, 204
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 136, 391
Bse8I GATNNNNATC 1 cut(s) 194
BseAI TCCGGA 1 cut(s) 204
BseDI CCNNGG 2 cut(s) 35, 59
BseGI GGATG 1 cut(s) 538
BseJI GATNNNNATC 1 cut(s) 194
BseLI CCNNNNNNNGG 3 cut(s) 103, 136, 391
BseRI GAGGAG 3 cut(s) 10, 239, 330
BseXI GCAGC 3 cut(s) 127, 368, 431
BsgI GTGCAG 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 44, 346
BshFI GGCC 1 cut(s) 539
BshNI GGYRCC 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 6 cut(s) 24, 183, 205, 215, 385, 495
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 3 cut(s) 103, 136, 391
BsmAI GTCTC 2 cut(s) 247, 313
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 4 cut(s) 46, 160, 307, 481
BsnI GGCC 1 cut(s) 539
Bso31I GGTCTC 1 cut(s) 313
BsoBI CYCGRG 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 18
Bsp13I TCCGGA 1 cut(s) 204
Bsp143I GATC 4 cut(s) 31, 307, 487, 520
BspANI GGCC 1 cut(s) 539
BspEI TCCGGA 1 cut(s) 204
BspFNI CGCG 2 cut(s) 44, 346
BspLI GGNNCC 3 cut(s) 339, 413, 538
BspPI GGATC 3 cut(s) 26, 315, 495
BspT104I TTCGAA 1 cut(s) 473
BspT107I GGYRCC 1 cut(s) 411
BspTNI GGTCTC 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 35, 59
BssMI GATC 4 cut(s) 31, 307, 487, 520
BssNI GRCGYC 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 60, 110
BstACI GRCGYC 1 cut(s) 412
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 1 cut(s) 42
BstDSI CCRYGG 2 cut(s) 35, 59
BstF5I GGATG 1 cut(s) 538
BstFNI CGCG 2 cut(s) 44, 346
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 2 cut(s) 46, 414
BstKTI GATC 4 cut(s) 34, 310, 490, 523
BstMAI GTCTC 2 cut(s) 247, 313
BstMBI GATC 4 cut(s) 31, 307, 487, 520
BstMWI GCNNNNNNNGC 5 cut(s) 146, 155, 311, 420, 545
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 3 cut(s) 214, 384, 494
BstSFI CTRYAG 1 cut(s) 480
BstUI CGCG 2 cut(s) 44, 346
BstV1I GCAGC 3 cut(s) 127, 368, 431
BstX2I RGATCY 2 cut(s) 307, 520
BstYI RGATCY 2 cut(s) 307, 520
BsuRI GGCC 1 cut(s) 539
BtgI CCRYGG 2 cut(s) 35, 59
BtsCI GGATG 1 cut(s) 538
Cac8I GCNNGC 1 cut(s) 42
CfoI GCGC 2 cut(s) 46, 414
Cfr13I GGNCC 1 cut(s) 537
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
CviAII CATG 2 cut(s) 74, 131
DinI GGCGCC 1 cut(s) 413
DpnI GATC 4 cut(s) 33, 309, 489, 522
DpnII GATC 4 cut(s) 31, 307, 487, 520
DrdI GACNNNNNNGTC 1 cut(s) 327
DseDI GACNNNNNNGTC 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 144
EgeI GGCGCC 1 cut(s) 413
EheI GGCGCC 1 cut(s) 413
FaeI CATG 2 cut(s) 77, 134
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 73, 130
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 4 cut(s) 141, 357, 442, 445
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 4 cut(s) 141, 357, 442, 445
GlaI GCGC 2 cut(s) 45, 413
GluI GCNGC 4 cut(s) 141, 357, 442, 445
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 1 cut(s) 539
HapII CCGG 6 cut(s) 24, 183, 205, 215, 385, 495
HhaI GCGC 2 cut(s) 46, 414
Hin1I GRCGYC 1 cut(s) 412
Hin1II CATG 2 cut(s) 77, 134
Hin6I GCGC 2 cut(s) 44, 412
HinP1I GCGC 2 cut(s) 44, 412
HinfI GANTC 3 cut(s) 171, 190, 365
HpaII CCGG 6 cut(s) 24, 183, 205, 215, 385, 495
Hpy188I TCNGA 1 cut(s) 236
Hpy188III TCNNGA 3 cut(s) 114, 205, 280
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 2 cut(s) 60, 110
HpyCH4V TGCA 1 cut(s) 447
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 155, 311, 420, 545
Hsp92I GRCGYC 1 cut(s) 412
Hsp92II CATG 2 cut(s) 77, 134
HspAI GCGC 2 cut(s) 44, 412
KasI GGCGCC 1 cut(s) 411
Kpn2I TCCGGA 1 cut(s) 204
Kzo9I GATC 4 cut(s) 31, 307, 487, 520
LmnI GCTCC 2 cut(s) 226, 343
LpnPI CCDG 6 cut(s) 37, 196, 218, 228, 398, 508
Lsp1109I GCAGC 3 cut(s) 127, 368, 431
LweI GCATC 1 cut(s) 467
MaeIII GTNAC 2 cut(s) 218, 395
MalI GATC 4 cut(s) 33, 309, 489, 522
MboI GATC 4 cut(s) 31, 307, 487, 520
MflI RGATCY 2 cut(s) 307, 520
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 2 cut(s) 469, 574
Mly113I GGCGCC 1 cut(s) 412
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 3 cut(s) 214, 220, 223
MnlI CCTC 6 cut(s) 31, 107, 110, 217, 351, 401
MroI TCCGGA 1 cut(s) 204
MseI TTAA 2 cut(s) 65, 266
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 444
MspI CCGG 6 cut(s) 24, 183, 205, 215, 385, 495
MspR9I CCNGG 3 cut(s) 216, 386, 496
Mva1269I GAATGC 4 cut(s) 46, 160, 307, 481
MvnI CGCG 2 cut(s) 44, 346
MwoI GCNNNNNNNGC 5 cut(s) 146, 155, 311, 420, 545
NarI GGCGCC 1 cut(s) 412
NciI CCSGG 3 cut(s) 216, 386, 496
NdeII GATC 4 cut(s) 31, 307, 487, 520
NlaIII CATG 2 cut(s) 77, 134
NlaIV GGNNCC 3 cut(s) 339, 413, 538
NmeAIII GCCGAG 2 cut(s) 274, 340
NmuCI GTSAC 1 cut(s) 395
NspI RCATGY 1 cut(s) 77
NspV TTCGAA 1 cut(s) 473
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PctI GAATGC 4 cut(s) 46, 160, 307, 481
PfeI GAWTC 2 cut(s) 171, 190
PflMI CCANNNNNTGG 1 cut(s) 136
PkrI GCNGC 4 cut(s) 142, 358, 443, 446
PleI GAGTC 1 cut(s) 359
PluTI GGCGCC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 339, 413, 538
PspPI GGNCC 1 cut(s) 537
PsuI RGATCY 2 cut(s) 307, 520
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 2 cut(s) 65, 266
SatI GCNGC 4 cut(s) 141, 357, 442, 445
Sau3AI GATC 4 cut(s) 31, 307, 487, 520
Sau96I GGNCC 1 cut(s) 537
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 3 cut(s) 216, 386, 496
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 1 cut(s) 467
SfcI CTRYAG 1 cut(s) 480
SfoI GGCGCC 1 cut(s) 413
SfuI TTCGAA 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 51
Sse9I AATT 2 cut(s) 469, 574
SspDI GGCGCC 1 cut(s) 411
StyD4I CCNGG 3 cut(s) 214, 384, 494
TaaI ACNGT 2 cut(s) 60, 110
TaqI TCGA 4 cut(s) 169, 279, 352, 473
TasI AATT 2 cut(s) 469, 574
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 2 cut(s) 171, 190
Tru1I TTAA 2 cut(s) 65, 266
Tru9I TTAA 2 cut(s) 65, 266
TseFI GTSAC 1 cut(s) 395
TseI GCWGC 3 cut(s) 140, 356, 444
Tsp45I GTSAC 1 cut(s) 395
TspDTI ATGAA 3 cut(s) 69, 84, 147
TspGWI ACGGA 2 cut(s) 24, 284
Van91I CCANNNNNTGG 1 cut(s) 136
XceI RCATGY 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.