Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000052.1

Orthogroup ID

OG0000314

Physical Location & Seq

Genomic Coordinates Forward (+)

64125 .. 65468

Gene Structure 1344 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_ssc0000052.1_g000016.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1287 bp

atggagaatgtcgagaaccatcgcaagaagatggacgcggatcttgccagagaaactgcaaaatcagaaaggctccaccgcgagtttgaacagcgtatgactctatacgtggagaataccaggcagcaggatgagcgtcataagacaattttgaatgctatagcggaacaaaacaagcatacctcattagaattcgcgaccttgtgcaaggaaggtcccagcttgactactcaagtcagttcacagcaaggcgaattggaaagccaaaaatcttcgcgcgaagaggttgtttctgaggttgaattgcctacagatggaaataaacctaaatgcctcattgatgagtcacggccttacatagaggctttgcatggagaagatcgtcaacaagattgttcctctgacaatataattgtttcagaacaaatatatgatttctccactgatcaagccaaatacgtggatgttaatcagatgcatggggggcatgatattgtccaggttcacccccttagtaaccagaagcagctgtatgatcaatttaattatggttatgctgctggacgtcatcatgaccaaggtaatcaaagccacaatcaacttaattatgaattaagttgttatatggctgctggacgtcctcatcatgcagaaggtcaagagtgtggtccccttaattatcaattaaggcatgggccacttgattatcaattatgttttggtcttgagaagccaatttgtggctttattaatcaattcaatttgaagatcttcatatatgtttcaaggccagttagtggctttgagatttgtaaagaaggattcggtgaccccaagtccagtcttgacatggatccaatctatgatgaatatgatgataattataatttaattattggttccaaaaatcatatgcaggagtacaagttctttgaagctcttgatagggagaatttagaccaacaagtggtcaaatacaaaagccaacctgcagtggctggttttgaagacaccatgttctatgacatggtagttggcgacaaagccgatcttgggacaacgtcatctccaaaatttcaattgaagatctcgcttcgtcaaccaacaaagatacttggggggcaatactccttctacgagccaaatttctttcaagttttcaacgcgaagtatctttgttattttcctacaagctctcacaagagggattttcaggctataaattttgacacatcggagcttggagtgaagcatagatggcctcccccgtggccttctagaggttag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004652 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006276 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016740 GO:0016772 GO:0016779 GO:0031123 GO:0031124 GO:0034641 GO:0043170 GO:0043412 GO:0043631 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0070566 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PolyA_pol PolyA_pol_RNAbd

Protein Analysis

428

Amino Acids

49.37

Weight (kDa)

5.41

Isoelectric Point (pI)

46.48

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000314)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02272 FvH4_1g22661 FvH4_2g10242 FvH4_2g34051 FvH4_3g19221 FvH4_3g29651 FvH4_3g30891 FvH4_4g11061 FvH4_5g09250 FvH4_5g15302 FvH4_5g15303 FvH4_5g15304 FvH4_5g28521 FvH4_6g23591 FvH4_7g05710 FvH4_7g19520
pyrus_communis	pycom07g06330
rosa_chinensis	RchiOBHm_Chr5g0027301 RchiOBHm_Chr6g0251451 RchiOBHm_Chr6g0274861
rosa_laevigata	RLG00000007850
rosa_multiflora	Rmu_co8230721.1_g000001 Rmu_sc0000271.1_g000027 Rmu_sc0000303.1_g000024 Rmu_sc0000368.1_g000024 Rmu_sc0000621.1_g000037 Rmu_sc0000693.1_g000081 Rmu_sc0001229.1_g000006 Rmu_sc0001373.1_g000003 Rmu_sc0001459.1_g000001 Rmu_sc0001744.1_g000002 Rmu_sc0001909.1_g000002 Rmu_sc0002053.1_g000012 Rmu_sc0002060.1_g000015 Rmu_sc0002119.1_g000036 Rmu_sc0002177.1_g000007 Rmu_sc0002822.1_g000024 Rmu_sc0002965.1_g000015 Rmu_sc0003363.1_g000051 Rmu_sc0003500.1_g000013 Rmu_sc0004191.1_g000020 Rmu_sc0004904.1_g000032 Rmu_sc0005325.1_g000008 Rmu_sc0005947.1_g000040 Rmu_sc0006475.1_g000001 Rmu_sc0006522.1_g000019 Rmu_sc0007102.1_g000005 Rmu_sc0007810.1_g000003 Rmu_sc0008339.1_g000025 Rmu_sc0008698.1_g000004 Rmu_sc0009313.1_g000006 Rmu_sc0009850.1_g000006 Rmu_sc0010598.1_g000003 Rmu_sc0011162.1_g000002 Rmu_sc0011824.1_g000004 Rmu_sc0011900.1_g000010 Rmu_sc0012291.1_g000006 Rmu_sc0012619.1_g000003 Rmu_sc0014679.1_g000001 Rmu_sc0019053.1_g000001 Rmu_sc0021462.1_g000003 Rmu_sc0024172.1_g000001 Rmu_sc0024173.1_g000001 Rmu_ssc0000052.1_g000016 Rmu_ssc0000392.1_g000010 Rmu_ssc0000421.1_g000062 Rmu_ssc0000479.1_g000006
rosa_roxburghii	Rroxscaffold_153G00436600 Rroxscaffold_1G00008580 Rroxscaffold_1G00016770 Rroxscaffold_1G00029550 Rroxscaffold_1G00036450 Rroxscaffold_1G00040410 Rroxscaffold_1G00047110 Rroxscaffold_1G00060570 Rroxscaffold_2G00081500 Rroxscaffold_2G00081510 Rroxscaffold_2G00086080 Rroxscaffold_2G00099620 Rroxscaffold_2G00099630 Rroxscaffold_2G00099640 Rroxscaffold_2G00109170 Rroxscaffold_2G00127750 Rroxscaffold_2G00128590 Rroxscaffold_2G00130780 Rroxscaffold_2G00131040 Rroxscaffold_2G00143160 Rroxscaffold_3G00229280 Rroxscaffold_3G00229290 Rroxscaffold_3G00229970 Rroxscaffold_3G00242860 Rroxscaffold_3G00242890 Rroxscaffold_3G00242900 Rroxscaffold_3G00248200 Rroxscaffold_4G00284550 Rroxscaffold_4G00288780 Rroxscaffold_4G00298620 Rroxscaffold_4G00298630 Rroxscaffold_4G00312420 Rroxscaffold_4G00320310 Rroxscaffold_4G00322220 Rroxscaffold_4G00323600 Rroxscaffold_4G00331830 Rroxscaffold_4G00332010 Rroxscaffold_4G00332020 Rroxscaffold_5G00344470 Rroxscaffold_5G00345310 Rroxscaffold_5G00356120 Rroxscaffold_5G00368700 Rroxscaffold_5G00379820 Rroxscaffold_5G00382480 Rroxscaffold_6G00395080 Rroxscaffold_6G00405520 Rroxscaffold_6G00428210 Rroxscaffold_7G00193460
rosa_samantha	Rh7CG413200 Rh7DG284900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	885
AatII	GACGTC	2 cut(s)	568, 640
Acc36I	ACCTGC	1 cut(s)	997
AccB7I	CCANNNNNTGG	3 cut(s)	740, 797, 967
AccII	CGCG	6 cut(s)	38, 81, 197, 277, 279, 1166
AciI	CCGC	3 cut(s)	38, 79, 164
AclWI	GGATC	3 cut(s)	48, 848, 861
AcsI	RAATTY	5 cut(s)	191, 952, 1073, 1144, 1222
AcyI	GRCGYC	2 cut(s)	565, 637
AfaI	GTAC	1 cut(s)	923
AfiI	CCNNNNNNNGG	6 cut(s)	314, 740, 797, 967, 1053, 1280
AhdI	GACNNNNNGTC	1 cut(s)	835
AjnI	CCWGG	2 cut(s)	119, 498
AloI	GAACNNNNNNTCC	2 cut(s)	911, 943
AluBI	AGCT	5 cut(s)	222, 529, 938, 1194, 1240
AluI	AGCT	5 cut(s)	222, 529, 938, 1194, 1240
AlwI	GGATC	3 cut(s)	48, 848, 861
AlwNI	CAGNNNCTG	1 cut(s)	998
AoxI	GGCC	5 cut(s)	350, 695, 788, 1259, 1271
ApeKI	GCWGC	4 cut(s)	124, 526, 557, 629
ApoI	RAATTY	5 cut(s)	191, 952, 1073, 1144, 1222
AseI	ATTAAT	1 cut(s)	750
Asp700I	GAANNNNTTC	1 cut(s)	770
AspLEI	GCGC	1 cut(s)	279
AspS9I	GGNCC	3 cut(s)	215, 668, 695
AsuHPI	GGTGA	2 cut(s)	497, 839
AvaII	GGWCC	2 cut(s)	215, 668
BamHI	GGATCC	1 cut(s)	853
BbsI	GAAGAC	1 cut(s)	1014
BbvI	GCAGC	4 cut(s)	136, 538, 544, 616
BccI	CCATC	4 cut(s)	25, 27, 308, 1251
BceAI	ACGGC	1 cut(s)	365
BciT130I	CCWGG	2 cut(s)	121, 500
BclI	TGATCA	2 cut(s)	445, 535
BfaI	CTAG	1 cut(s)	1278
BfmI	CTRYAG	3 cut(s)	159, 309, 990
BfuAI	ACCTGC	1 cut(s)	997
BglII	AGATCT	2 cut(s)	768, 1086
BisI	GCNGC	4 cut(s)	125, 527, 558, 630
BlsI	GCNGC	4 cut(s)	126, 528, 559, 631
Bme1390I	CCNGG	2 cut(s)	121, 500
Bme18I	GGWCC	2 cut(s)	215, 668
BmeRI	GACNNNNNGTC	1 cut(s)	835
BmgT120I	GGNCC	3 cut(s)	215, 668, 695
BmiI	GGNNCC	5 cut(s)	74, 217, 670, 855, 901
BmrFI	CCNGG	2 cut(s)	121, 500
BmsI	GCATC	1 cut(s)	465
BpiI	GAAGAC	1 cut(s)	1014
BpuEI	CTTGAG	2 cut(s)	216, 746
BsaAI	YACGTR	2 cut(s)	109, 460
BsaBI	GATNNNNATC	1 cut(s)	468
BsaHI	GRCGYC	2 cut(s)	565, 637
BsaJI	CCNNGG	2 cut(s)	577, 1268
BsaXI	ACNNNNNCTCC	4 cut(s)	911, 941, 1051, 1081
Bsc4I	CCNNNNNNNGG	6 cut(s)	314, 740, 797, 967, 1053, 1280
Bse1I	ACTGG	2 cut(s)	791, 840
Bse8I	GATNNNNATC	1 cut(s)	468
BseBI	CCWGG	2 cut(s)	121, 500
BseDI	CCNNGG	2 cut(s)	577, 1268
BseGI	GGATG	2 cut(s)	136, 469
BseJI	GATNNNNATC	1 cut(s)	468
BseLI	CCNNNNNNNGG	6 cut(s)	314, 740, 797, 967, 1053, 1280
BseMII	CTCAG	1 cut(s)	285
BseNI	ACTGG	2 cut(s)	791, 840
BseXI	GCAGC	4 cut(s)	136, 538, 544, 616
BseYI	CCCAGC	1 cut(s)	218
Bsh1236I	CGCG	6 cut(s)	38, 81, 197, 277, 279, 1166
BshFI	GGCC	5 cut(s)	352, 697, 790, 1261, 1273
BslFI	GGGAC	3 cut(s)	201, 654, 1069
BslI	CCNNNNNNNGG	6 cut(s)	314, 740, 797, 967, 1053, 1280
BsmFI	GGGAC	3 cut(s)	201, 654, 1069
BsmI	GAATGC	1 cut(s)	160
BsnI	GGCC	5 cut(s)	352, 697, 790, 1261, 1273
Bsp143I	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
Bsp68I	TCGCGA	1 cut(s)	197
BspACI	CCGC	3 cut(s)	38, 79, 164
BspANI	GGCC	5 cut(s)	352, 697, 790, 1261, 1273
BspCNI	CTCAG	1 cut(s)	286
BspFNI	CGCG	6 cut(s)	38, 81, 197, 277, 279, 1166
BspHI	TCATGA	1 cut(s)	571
BspLI	GGNNCC	5 cut(s)	74, 217, 670, 855, 901
BspMAI	CTGCAG	1 cut(s)	994
BspMI	ACCTGC	1 cut(s)	997
BspPI	GGATC	3 cut(s)	48, 848, 861
BsrI	ACTGG	2 cut(s)	791, 840
BssECI	CCNNGG	2 cut(s)	577, 1268
BssMI	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
BssNI	GRCGYC	2 cut(s)	565, 637
BssT1I	CCWWGG	1 cut(s)	577
Bst2UI	CCWGG	2 cut(s)	121, 500
Bst6I	CTCTTC	1 cut(s)	276
BstACI	GRCGYC	2 cut(s)	565, 637
BstBAI	YACGTR	2 cut(s)	109, 460
BstDEI	CTNAG	2 cut(s)	294, 512
BstDSI	CCRYGG	1 cut(s)	1268
BstEII	GGTNACC	1 cut(s)	827
BstENI	CCTNNNNNAGG	1 cut(s)	1278
BstF5I	GGATG	2 cut(s)	136, 469
BstFNI	CGCG	6 cut(s)	38, 81, 197, 277, 279, 1166
BstHHI	GCGC	1 cut(s)	279
BstKTI	GATC	8 cut(s)	43, 382, 448, 538, 771, 856, 1051, 1089
BstMBI	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
BstMWI	GCNNNNNNNGC	4 cut(s)	44, 133, 484, 1258
BstNI	CCWGG	2 cut(s)	121, 500
BstPI	GGTNACC	1 cut(s)	827
BstSCI	CCNGG	2 cut(s)	119, 498
BstSFI	CTRYAG	3 cut(s)	159, 309, 990
BstUI	CGCG	6 cut(s)	38, 81, 197, 277, 279, 1166
BstV1I	GCAGC	4 cut(s)	136, 538, 544, 616
BstV2I	GAAGAC	1 cut(s)	1014
BstX2I	RGATCY	4 cut(s)	40, 768, 853, 1086
BstXI	CCANNNNNNTGG	1 cut(s)	460
BstYI	RGATCY	4 cut(s)	40, 768, 853, 1086
BsuRI	GGCC	5 cut(s)	352, 697, 790, 1261, 1273
BtgI	CCRYGG	1 cut(s)	1268
BtgZI	GCGATG	1 cut(s)	5
BtsCI	GGATG	2 cut(s)	136, 469
BtsI	GCAGTG	1 cut(s)	999
BtsIMutI	CAGTG	2 cut(s)	441, 999
BtuMI	TCGCGA	1 cut(s)	197
BveI	ACCTGC	1 cut(s)	997
CaiI	CAGNNNCTG	1 cut(s)	998
CciI	TCATGA	1 cut(s)	571
CfoI	GCGC	1 cut(s)	279
Cfr13I	GGNCC	3 cut(s)	215, 668, 695
CseI	GACGC	2 cut(s)	44, 125
Csp6I	GTAC	1 cut(s)	922
CviAII	CATG	9 cut(s)	371, 479, 488, 572, 647, 692, 850, 1015, 1027
CviQI	GTAC	1 cut(s)	922
DdeI	CTNAG	2 cut(s)	294, 512
DpnI	GATC	8 cut(s)	42, 381, 447, 537, 770, 855, 1050, 1088
DpnII	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
DriI	GACNNNNNGTC	1 cut(s)	835
Eam1104I	CTCTTC	1 cut(s)	276
Eam1105I	GACNNNNNGTC	1 cut(s)	835
EarI	CTCTTC	1 cut(s)	276
Eco130I	CCWWGG	1 cut(s)	577
Eco47I	GGWCC	2 cut(s)	215, 668
Eco91I	GGTNACC	1 cut(s)	827
EcoNI	CCTNNNNNAGG	1 cut(s)	1278
EcoO109I	RGGNCCY	1 cut(s)	215
EcoO65I	GGTNACC	1 cut(s)	827
EcoRI	GAATTC	1 cut(s)	191
EcoRII	CCWGG	2 cut(s)	119, 498
EcoT14I	CCWWGG	1 cut(s)	577
EcoT22I	ATGCAT	1 cut(s)	480
ErhI	CCWWGG	1 cut(s)	577
FaeI	CATG	9 cut(s)	374, 482, 491, 575, 650, 695, 853, 1018, 1030
FalI	AAGNNNNNCTT	2 cut(s)	1035, 1067
FaqI	GGGAC	3 cut(s)	201, 654, 1069
FatI	CATG	9 cut(s)	370, 478, 487, 571, 646, 691, 849, 1014, 1026
FauNDI	CATATG	1 cut(s)	912
FbaI	TGATCA	2 cut(s)	445, 535
Fnu4HI	GCNGC	4 cut(s)	125, 527, 558, 630
FokI	GGATG	2 cut(s)	143, 476
Fsp4HI	GCNGC	4 cut(s)	125, 527, 558, 630
FspBI	CTAG	1 cut(s)	1278
GlaI	GCGC	1 cut(s)	278
GluI	GCNGC	4 cut(s)	125, 527, 558, 630
GsaI	CCCAGC	1 cut(s)	222
HaeIII	GGCC	5 cut(s)	352, 697, 790, 1261, 1273
HgaI	GACGC	2 cut(s)	44, 125
HhaI	GCGC	1 cut(s)	279
Hin1I	GRCGYC	2 cut(s)	565, 637
Hin1II	CATG	9 cut(s)	374, 482, 491, 575, 650, 695, 853, 1018, 1030
Hin6I	GCGC	1 cut(s)	277
HinP1I	GCGC	1 cut(s)	277
HincII	GTYRAC	2 cut(s)	386, 1100
HindII	GTYRAC	2 cut(s)	386, 1100
HinfI	GANTC	3 cut(s)	100, 344, 822
HphI	GGTGA	2 cut(s)	497, 839
Hpy166II	GTNNAC	4 cut(s)	242, 386, 505, 1100
Hpy188I	TCNGA	6 cut(s)	67, 295, 403, 421, 474, 1237
Hpy188III	TCNNGA	8 cut(s)	13, 196, 572, 659, 725, 845, 941, 1278
Hpy8I	GTNNAC	4 cut(s)	242, 386, 505, 1100
HpyAV	CCTTC	5 cut(s)	206, 647, 812, 1141, 1284
HpyCH4IV	ACGT	5 cut(s)	108, 459, 565, 637, 1061
HpyCH4V	TGCA	7 cut(s)	59, 207, 370, 478, 650, 916, 992
HpyF10VI	GCNNNNNNNGC	4 cut(s)	44, 133, 484, 1258
HpyF3I	CTNAG	2 cut(s)	294, 512
HpySE526I	ACGT	5 cut(s)	108, 459, 565, 637, 1061
Hsp92I	GRCGYC	2 cut(s)	565, 637
Hsp92II	CATG	9 cut(s)	374, 482, 491, 575, 650, 695, 853, 1018, 1030
HspAI	GCGC	1 cut(s)	277
Ksp22I	TGATCA	2 cut(s)	445, 535
Kzo9I	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
LmnI	GCTCC	2 cut(s)	78, 1237
Lsp1109I	GCAGC	4 cut(s)	136, 538, 544, 616
LweI	GCATC	1 cut(s)	465
MaeI	CTAG	1 cut(s)	1278
MaeII	ACGT	5 cut(s)	108, 459, 565, 637, 1061
MaeIII	GTNAC	3 cut(s)	345, 515, 827
MalI	GATC	8 cut(s)	42, 381, 447, 537, 770, 855, 1050, 1088
MboI	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
MboII	GAAGA	8 cut(s)	40, 264, 293, 389, 763, 778, 1019, 1096
MfeI	CAATTG	1 cut(s)	1079
MflI	RGATCY	4 cut(s)	40, 768, 853, 1086
MlyI	GAGTC	2 cut(s)	94, 353
Mph1103I	ATGCAT	1 cut(s)	480
MroXI	GAANNNNTTC	1 cut(s)	770
MseI	TTAA	8 cut(s)	468, 543, 603, 614, 675, 686, 750, 890
MspA1I	CMGCKG	1 cut(s)	529
MspR9I	CCNGG	2 cut(s)	121, 500
MunI	CAATTG	1 cut(s)	1079
Mva1269I	GAATGC	1 cut(s)	160
MvaI	CCWGG	2 cut(s)	121, 500
MvnI	CGCG	6 cut(s)	38, 81, 197, 277, 279, 1166
MwoI	GCNNNNNNNGC	4 cut(s)	44, 133, 484, 1258
NdeI	CATATG	1 cut(s)	912
NdeII	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
NlaIII	CATG	9 cut(s)	374, 482, 491, 575, 650, 695, 853, 1018, 1030
NlaIV	GGNNCC	5 cut(s)	74, 217, 670, 855, 901
NmuCI	GTSAC	2 cut(s)	345, 827
NruI	TCGCGA	1 cut(s)	197
NsiI	ATGCAT	1 cut(s)	480
PagI	TCATGA	1 cut(s)	571
PctI	GAATGC	1 cut(s)	160
PdmI	GAANNNNTTC	1 cut(s)	770
PfeI	GAWTC	1 cut(s)	822
PflFI	GACNNNGTC	1 cut(s)	1060
PflMI	CCANNNNNTGG	3 cut(s)	740, 797, 967
PkrI	GCNGC	4 cut(s)	126, 528, 559, 631
PleI	GAGTC	2 cut(s)	94, 352
PpsI	GAGTC	2 cut(s)	94, 352
Ppu21I	YACGTR	2 cut(s)	109, 460
PpuMI	RGGWCCY	1 cut(s)	215
PshBI	ATTAAT	1 cut(s)	750
PsiI	TTATAA	1 cut(s)	885
Psp5II	RGGWCCY	1 cut(s)	215
Psp6I	CCWGG	2 cut(s)	119, 498
PspEI	GGTNACC	1 cut(s)	827
PspFI	CCCAGC	1 cut(s)	218
PspGI	CCWGG	2 cut(s)	119, 498
PspN4I	GGNNCC	5 cut(s)	74, 217, 670, 855, 901
PspPI	GGNCC	3 cut(s)	215, 668, 695
PspPPI	RGGWCCY	1 cut(s)	215
PstI	CTGCAG	1 cut(s)	994
PstNI	CAGNNNCTG	1 cut(s)	998
PsuI	RGATCY	4 cut(s)	40, 768, 853, 1086
PsyI	GACNNNGTC	1 cut(s)	1060
PvuII	CAGCTG	1 cut(s)	529
RruI	TCGCGA	1 cut(s)	197
RsaI	GTAC	1 cut(s)	923
RsaNI	GTAC	1 cut(s)	922
SaqAI	TTAA	8 cut(s)	468, 543, 603, 614, 675, 686, 750, 890
SatI	GCNGC	4 cut(s)	125, 527, 558, 630
Sau3AI	GATC	8 cut(s)	40, 379, 445, 535, 768, 853, 1048, 1086
Sau96I	GGNCC	3 cut(s)	215, 668, 695
SchI	GAGTC	2 cut(s)	94, 353
ScrFI	CCNGG	2 cut(s)	121, 500
SfaNI	GCATC	1 cut(s)	465
SfcI	CTRYAG	3 cut(s)	159, 309, 990
SinI	GGWCC	2 cut(s)	215, 668
SmlI	CTYRAG	2 cut(s)	231, 725
SmoI	CTYRAG	2 cut(s)	231, 725
SsiI	CCGC	3 cut(s)	38, 79, 164
SspMI	CTAG	1 cut(s)	1278
StyD4I	CCNGG	2 cut(s)	119, 498
StyI	CCWWGG	1 cut(s)	577
TaiI	ACGT	5 cut(s)	111, 462, 568, 640, 1064
TaqI	TCGA	1 cut(s)	12
TatI	WGTACW	1 cut(s)	921
TfiI	GAWTC	1 cut(s)	822
Tru1I	TTAA	8 cut(s)	468, 543, 603, 614, 675, 686, 750, 890
Tru9I	TTAA	8 cut(s)	468, 543, 603, 614, 675, 686, 750, 890
TscAI	CASTG	2 cut(s)	448, 999
TseFI	GTSAC	2 cut(s)	345, 827
TseI	GCWGC	4 cut(s)	124, 526, 557, 629
Tsp45I	GTSAC	2 cut(s)	345, 827
TspDTI	ATGAA	3 cut(s)	624, 763, 882
TspRI	CASTG	2 cut(s)	448, 999
Tth111I	GACNNNGTC	1 cut(s)	1060
Van91I	CCANNNNNTGG	3 cut(s)	740, 797, 967
VpaK11BI	GGWCC	2 cut(s)	215, 668
VspI	ATTAAT	1 cut(s)	750
XagI	CCTNNNNNAGG	1 cut(s)	1278
XapI	RAATTY	5 cut(s)	191, 952, 1073, 1144, 1222
XbaI	TCTAGA	1 cut(s)	1277
XcmI	CCANNNNNNNNNTGG	1 cut(s)	847
XmnI	GAANNNNTTC	1 cut(s)	770
XspI	CTAG	1 cut(s)	1278
ZraI	GACGTC	2 cut(s)	566, 638
Zsp2I	ATGCAT	1 cut(s)	480

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.