Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1557 |
| AatII | GACGTC | 4 cut(s) | 727, 847, 967, 1087 |
| Acc36I | ACCTGC | 1 cut(s) | 220 |
| AccB1I | GGYRCC | 1 cut(s) | 248 |
| AccB7I | CCANNNNNTGG | 4 cut(s) | 1157, 1214, 1639, 1663 |
| AccII | CGCG | 9 cut(s) | 95, 140, 183, 212, 356, 436, 438, 1302, 1835 |
| AciI | CCGC | 7 cut(s) | 140, 178, 181, 212, 323, 1212, 1245 |
| AclWI | GGATC | 4 cut(s) | 150, 792, 912, 1032 |
| AcoI | YGGCCR | 7 cut(s) | 3, 829, 949, 1069, 1111, 1264, 1285 |
| AcsI | RAATTY | 6 cut(s) | 1310, 1373, 1492, 1813, 1876, 1891 |
| AcuI | CTGAAG | 1 cut(s) | 1695 |
| AcyI | GRCGYC | 6 cut(s) | 724, 844, 964, 1084, 1409, 1422 |
| AfaI | GTAC | 3 cut(s) | 780, 900, 1595 |
| AjnI | CCWGG | 3 cut(s) | 221, 657, 1424 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1281, 1313 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1583, 1615 |
| AluBI | AGCT | 2 cut(s) | 266, 1863 |
| AluI | AGCT | 2 cut(s) | 266, 1863 |
| Alw21I | GWGCWC | 1 cut(s) | 221 |
| Alw26I | GTCTC | 3 cut(s) | 80, 580, 1416 |
| AlwI | GGATC | 4 cut(s) | 150, 792, 912, 1032 |
| ApeKI | GCWGC | 3 cut(s) | 193, 226, 278 |
| ApoI | RAATTY | 6 cut(s) | 1310, 1373, 1492, 1813, 1876, 1891 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1295, 1327 |
| AseI | ATTAAT | 2 cut(s) | 1167, 1482 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1376 |
| AspLEI | GCGC | 2 cut(s) | 262, 438 |
| AspS9I | GGNCC | 5 cut(s) | 374, 800, 920, 1040, 1247 |
| AsuC2I | CCSGG | 1 cut(s) | 65 |
| AsuHPI | GGTGA | 5 cut(s) | 656, 806, 926, 1046, 1511 |
| AsuII | TTCGAA | 1 cut(s) | 53 |
| AvaII | GGWCC | 1 cut(s) | 374 |
| BalI | TGGCCA | 7 cut(s) | 5, 831, 951, 1071, 1113, 1266, 1287 |
| BanI | GGYRCC | 1 cut(s) | 248 |
| BbsI | GAAGAC | 1 cut(s) | 1683 |
| Bbv12I | GWGCWC | 1 cut(s) | 221 |
| BbvI | GCAGC | 3 cut(s) | 205, 238, 265 |
| BccI | CCATC | 9 cut(s) | 127, 129, 281, 467, 533, 811, 931, 1051, 1920 |
| BceAI | ACGGC | 1 cut(s) | 524 |
| BciT130I | CCWGG | 3 cut(s) | 223, 659, 1426 |
| BciVI | GTATCC | 1 cut(s) | 1229 |
| BclI | TGATCA | 4 cut(s) | 604, 628, 1099, 1615 |
| BcnI | CCSGG | 1 cut(s) | 65 |
| BcoDI | GTCTC | 3 cut(s) | 80, 580, 1416 |
| BfaI | CTAG | 2 cut(s) | 1281, 1947 |
| BfmI | CTRYAG | 2 cut(s) | 318, 468 |
| BfuAI | ACCTGC | 1 cut(s) | 220 |
| BfuI | GTATCC | 1 cut(s) | 1229 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 257 |
| BglII | AGATCT | 1 cut(s) | 1755 |
| BisI | GCNGC | 5 cut(s) | 181, 194, 227, 279, 1246 |
| BlsI | GCNGC | 5 cut(s) | 182, 195, 228, 280, 1247 |
| Bme1390I | CCNGG | 4 cut(s) | 65, 223, 659, 1426 |
| Bme18I | GGWCC | 1 cut(s) | 374 |
| BmgT120I | GGNCC | 5 cut(s) | 374, 800, 920, 1040, 1247 |
| BmiI | GGNNCC | 4 cut(s) | 176, 250, 376, 1573 |
| BmrFI | CCNGG | 4 cut(s) | 65, 223, 659, 1426 |
| BmsI | GCATC | 2 cut(s) | 624, 1510 |
| BpiI | GAAGAC | 1 cut(s) | 1683 |
| Bpu14I | TTCGAA | 1 cut(s) | 53 |
| BpuEI | CTTGAG | 1 cut(s) | 375 |
| BpuMI | CCSGG | 1 cut(s) | 65 |
| BsaAI | YACGTR | 1 cut(s) | 619 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1104 |
| BsaHI | GRCGYC | 6 cut(s) | 724, 844, 964, 1084, 1409, 1422 |
| BsaJI | CCNNGG | 5 cut(s) | 657, 736, 856, 976, 1937 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1583, 1613 |
| Bse1I | ACTGG | 4 cut(s) | 724, 844, 964, 1084 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1104 |
| BseBI | CCWGG | 3 cut(s) | 223, 659, 1426 |
| BseDI | CCNNGG | 5 cut(s) | 657, 736, 856, 976, 1937 |
| BseGI | GGATG | 1 cut(s) | 295 |
| BseJI | GATNNNNATC | 1 cut(s) | 1104 |
| BseMII | CTCAG | 2 cut(s) | 444, 591 |
| BseNI | ACTGG | 4 cut(s) | 724, 844, 964, 1084 |
| BseRI | GAGGAG | 3 cut(s) | 98, 1490, 1923 |
| BseXI | GCAGC | 3 cut(s) | 205, 238, 265 |
| Bsh1236I | CGCG | 9 cut(s) | 95, 140, 183, 212, 356, 436, 438, 1302, 1835 |
| BshNI | GGYRCC | 1 cut(s) | 248 |
| BsiHKAI | GWGCWC | 1 cut(s) | 221 |
| BsiSI | CCGG | 1 cut(s) | 65 |
| BslFI | GGGAC | 2 cut(s) | 360, 1738 |
| BsmAI | GTCTC | 3 cut(s) | 80, 580, 1416 |
| BsmBI | CGTCTC | 1 cut(s) | 1416 |
| BsmFI | GGGAC | 2 cut(s) | 360, 1738 |
| Bsp119I | TTCGAA | 1 cut(s) | 53 |
| Bsp1286I | GDGCHC | 1 cut(s) | 221 |
| Bsp68I | TCGCGA | 3 cut(s) | 95, 356, 1302 |
| BspACI | CCGC | 7 cut(s) | 140, 178, 181, 212, 323, 1212, 1245 |
| BspCNI | CTCAG | 2 cut(s) | 445, 590 |
| BspFNI | CGCG | 9 cut(s) | 95, 140, 183, 212, 356, 436, 438, 1302, 1835 |
| BspHI | TCATGA | 1 cut(s) | 1618 |
| BspLI | GGNNCC | 4 cut(s) | 176, 250, 376, 1573 |
| BspMI | ACCTGC | 1 cut(s) | 220 |
| BspPI | GGATC | 4 cut(s) | 150, 792, 912, 1032 |
| BspT104I | TTCGAA | 1 cut(s) | 53 |
| BspT107I | GGYRCC | 1 cut(s) | 248 |
| BsrI | ACTGG | 4 cut(s) | 724, 844, 964, 1084 |
| BssECI | CCNNGG | 5 cut(s) | 657, 736, 856, 976, 1937 |
| BssNI | GRCGYC | 6 cut(s) | 724, 844, 964, 1084, 1409, 1422 |
| BssT1I | CCWWGG | 3 cut(s) | 736, 856, 976 |
| Bst2UI | CCWGG | 3 cut(s) | 223, 659, 1426 |
| Bst4CI | ACNGT | 3 cut(s) | 1196, 1349, 1398 |
| Bst6I | CTCTTC | 2 cut(s) | 33, 435 |
| BstACI | GRCGYC | 6 cut(s) | 724, 844, 964, 1084, 1409, 1422 |
| BstBAI | YACGTR | 1 cut(s) | 619 |
| BstBI | TTCGAA | 1 cut(s) | 53 |
| BstC8I | GCNNGC | 1 cut(s) | 264 |
| BstDEI | CTNAG | 2 cut(s) | 453, 577 |
| BstDSI | CCRYGG | 1 cut(s) | 1937 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1947 |
| BstF5I | GGATG | 1 cut(s) | 295 |
| BstFNI | CGCG | 9 cut(s) | 95, 140, 183, 212, 356, 436, 438, 1302, 1835 |
| BstHHI | GCGC | 2 cut(s) | 262, 438 |
| BstMAI | GTCTC | 3 cut(s) | 80, 580, 1416 |
| BstNI | CCWGG | 3 cut(s) | 223, 659, 1426 |
| BstSCI | CCNGG | 4 cut(s) | 63, 221, 657, 1424 |
| BstSFI | CTRYAG | 2 cut(s) | 318, 468 |
| BstUI | CGCG | 9 cut(s) | 95, 140, 183, 212, 356, 436, 438, 1302, 1835 |
| BstV1I | GCAGC | 3 cut(s) | 205, 238, 265 |
| BstV2I | GAAGAC | 1 cut(s) | 1683 |
| BstX2I | RGATCY | 2 cut(s) | 142, 1755 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 619, 839, 959, 1079 |
| BstYI | RGATCY | 2 cut(s) | 142, 1755 |
| BsuI | GTATCC | 1 cut(s) | 1229 |
| BtgI | CCRYGG | 1 cut(s) | 1937 |
| BtgZI | GCGATG | 1 cut(s) | 259 |
| BtsCI | GGATG | 1 cut(s) | 295 |
| BtsIMutI | CAGTG | 2 cut(s) | 600, 1201 |
| BtuMI | TCGCGA | 3 cut(s) | 95, 356, 1302 |
| BveI | ACCTGC | 1 cut(s) | 220 |
| Cac8I | GCNNGC | 1 cut(s) | 264 |
| CciI | TCATGA | 1 cut(s) | 1618 |
| CfoI | GCGC | 2 cut(s) | 262, 438 |
| Cfr13I | GGNCC | 5 cut(s) | 374, 800, 920, 1040, 1247 |
| CseI | GACGC | 4 cut(s) | 146, 284, 1398, 1430 |
| Csp6I | GTAC | 3 cut(s) | 779, 899, 1594 |
| CviQI | GTAC | 3 cut(s) | 779, 899, 1594 |
| DdeI | CTNAG | 2 cut(s) | 453, 577 |
| EaeI | YGGCCR | 7 cut(s) | 3, 829, 949, 1069, 1111, 1264, 1285 |
| Eam1104I | CTCTTC | 2 cut(s) | 33, 435 |
| EarI | CTCTTC | 2 cut(s) | 33, 435 |
| EciI | GGCGGA | 1 cut(s) | 167 |
| Eco130I | CCWWGG | 3 cut(s) | 736, 856, 976 |
| Eco47I | GGWCC | 1 cut(s) | 374 |
| Eco57I | CTGAAG | 1 cut(s) | 1695 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1947 |
| EcoO109I | RGGNCCY | 1 cut(s) | 374 |
| EcoRII | CCWGG | 3 cut(s) | 221, 657, 1424 |
| EcoT14I | CCWWGG | 3 cut(s) | 736, 856, 976 |
| EcoT22I | ATGCAT | 1 cut(s) | 639 |
| ErhI | CCWWGG | 3 cut(s) | 736, 856, 976 |
| Esp3I | CGTCTC | 1 cut(s) | 1416 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1704, 1736 |
| FaqI | GGGAC | 2 cut(s) | 360, 1738 |
| FauNDI | CATATG | 1 cut(s) | 1584 |
| FbaI | TGATCA | 4 cut(s) | 604, 628, 1099, 1615 |
| Fnu4HI | GCNGC | 5 cut(s) | 181, 194, 227, 279, 1246 |
| FokI | GGATG | 1 cut(s) | 302 |
| Fsp4HI | GCNGC | 5 cut(s) | 181, 194, 227, 279, 1246 |
| FspBI | CTAG | 2 cut(s) | 1281, 1947 |
| GlaI | GCGC | 2 cut(s) | 261, 437 |
| GluI | GCNGC | 5 cut(s) | 181, 194, 227, 279, 1246 |
| HapII | CCGG | 1 cut(s) | 65 |
| HgaI | GACGC | 4 cut(s) | 146, 284, 1398, 1430 |
| HhaI | GCGC | 2 cut(s) | 262, 438 |
| Hin1I | GRCGYC | 6 cut(s) | 724, 844, 964, 1084, 1409, 1422 |
| Hin6I | GCGC | 2 cut(s) | 260, 436 |
| HinP1I | GCGC | 2 cut(s) | 260, 436 |
| HincII | GTYRAC | 3 cut(s) | 545, 1335, 1769 |
| HindII | GTYRAC | 3 cut(s) | 545, 1335, 1769 |
| HinfI | GANTC | 4 cut(s) | 97, 202, 503, 1502 |
| HpaI | GTTAAC | 1 cut(s) | 1335 |
| HpaII | CCGG | 1 cut(s) | 65 |
| HphI | GGTGA | 5 cut(s) | 656, 806, 926, 1046, 1511 |
| Hpy166II | GTNNAC | 6 cut(s) | 401, 545, 664, 1335, 1345, 1769 |
| Hpy188I | TCNGA | 9 cut(s) | 169, 349, 454, 562, 580, 633, 1474, 1675, 1906 |
| Hpy8I | GTNNAC | 6 cut(s) | 401, 545, 664, 1335, 1345, 1769 |
| Hpy99I | CGWCG | 1 cut(s) | 1906 |
| HpyAV | CCTTC | 6 cut(s) | 62, 88, 250, 365, 1810, 1953 |
| HpyCH4III | ACNGT | 3 cut(s) | 1196, 1349, 1398 |
| HpyCH4IV | ACGT | 6 cut(s) | 618, 724, 844, 964, 1084, 1901 |
| HpyF3I | CTNAG | 2 cut(s) | 453, 577 |
| HpySE526I | ACGT | 6 cut(s) | 618, 724, 844, 964, 1084, 1901 |
| Hsp92I | GRCGYC | 6 cut(s) | 724, 844, 964, 1084, 1409, 1422 |
| HspAI | GCGC | 2 cut(s) | 260, 436 |
| Ksp22I | TGATCA | 4 cut(s) | 604, 628, 1099, 1615 |
| KspAI | GTTAAC | 1 cut(s) | 1335 |
| LmnI | GCTCC | 2 cut(s) | 71, 180 |
| Lsp1109I | GCAGC | 3 cut(s) | 205, 238, 265 |
| LweI | GCATC | 2 cut(s) | 624, 1510 |
| MaeI | CTAG | 2 cut(s) | 1281, 1947 |
| MaeII | ACGT | 6 cut(s) | 618, 724, 844, 964, 1084, 1901 |
| MaeIII | GTNAC | 2 cut(s) | 232, 504 |
| MboII | GAAGA | 9 cut(s) | 50, 142, 423, 452, 548, 1180, 1688, 1765, 1810 |
| MfeI | CAATTG | 1 cut(s) | 1748 |
| MflI | RGATCY | 2 cut(s) | 142, 1755 |
| MhlI | GDGCHC | 1 cut(s) | 221 |
| MlsI | TGGCCA | 7 cut(s) | 5, 831, 951, 1071, 1113, 1266, 1287 |
| MluNI | TGGCCA | 7 cut(s) | 5, 831, 951, 1071, 1113, 1266, 1287 |
| MlyI | GAGTC | 2 cut(s) | 196, 512 |
| MmeI | TCCRAC | 1 cut(s) | 1884 |
| Mox20I | TGGCCA | 7 cut(s) | 5, 831, 951, 1071, 1113, 1266, 1287 |
| Mph1103I | ATGCAT | 1 cut(s) | 639 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1376 |
| MscI | TGGCCA | 7 cut(s) | 5, 831, 951, 1071, 1113, 1266, 1287 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1107 |
| Msp20I | TGGCCA | 7 cut(s) | 5, 831, 951, 1071, 1113, 1266, 1287 |
| MspI | CCGG | 1 cut(s) | 65 |
| MspR9I | CCNGG | 4 cut(s) | 65, 223, 659, 1426 |
| MunI | CAATTG | 1 cut(s) | 1748 |
| MvaI | CCWGG | 3 cut(s) | 223, 659, 1426 |
| MvnI | CGCG | 9 cut(s) | 95, 140, 183, 212, 356, 436, 438, 1302, 1835 |
| NciI | CCSGG | 1 cut(s) | 65 |
| NdeI | CATATG | 1 cut(s) | 1584 |
| NlaIV | GGNNCC | 4 cut(s) | 176, 250, 376, 1573 |
| NmuCI | GTSAC | 1 cut(s) | 504 |
| NruI | TCGCGA | 3 cut(s) | 95, 356, 1302 |
| NsiI | ATGCAT | 1 cut(s) | 639 |
| NspV | TTCGAA | 1 cut(s) | 53 |
| PagI | TCATGA | 1 cut(s) | 1618 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1376 |
| PfeI | GAWTC | 2 cut(s) | 97, 1502 |
| PflMI | CCANNNNNTGG | 4 cut(s) | 1157, 1214, 1639, 1663 |
| PfoI | TCCNGGA | 1 cut(s) | 63 |
| PkrI | GCNGC | 5 cut(s) | 182, 195, 228, 280, 1247 |
| PleI | GAGTC | 2 cut(s) | 196, 511 |
| PpsI | GAGTC | 2 cut(s) | 196, 511 |
| Ppu21I | YACGTR | 1 cut(s) | 619 |
| PpuMI | RGGWCCY | 1 cut(s) | 374 |
| PshBI | ATTAAT | 2 cut(s) | 1167, 1482 |
| PsiI | TTATAA | 1 cut(s) | 1557 |
| Psp5II | RGGWCCY | 1 cut(s) | 374 |
| Psp6I | CCWGG | 3 cut(s) | 221, 657, 1424 |
| PspGI | CCWGG | 3 cut(s) | 221, 657, 1424 |
| PspN4I | GGNNCC | 4 cut(s) | 176, 250, 376, 1573 |
| PspPI | GGNCC | 5 cut(s) | 374, 800, 920, 1040, 1247 |
| PspPPI | RGGWCCY | 1 cut(s) | 374 |
| PsuI | RGATCY | 2 cut(s) | 142, 1755 |
| RruI | TCGCGA | 3 cut(s) | 95, 356, 1302 |
| RsaI | GTAC | 3 cut(s) | 780, 900, 1595 |
| RsaNI | GTAC | 3 cut(s) | 779, 899, 1594 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1107 |
| SatI | GCNGC | 5 cut(s) | 181, 194, 227, 279, 1246 |
| Sau96I | GGNCC | 5 cut(s) | 374, 800, 920, 1040, 1247 |
| SchI | GAGTC | 2 cut(s) | 196, 512 |
| ScrFI | CCNGG | 4 cut(s) | 65, 223, 659, 1426 |
| SduI | GDGCHC | 1 cut(s) | 221 |
| SfaNI | GCATC | 2 cut(s) | 624, 1510 |
| SfcI | CTRYAG | 2 cut(s) | 318, 468 |
| SfuI | TTCGAA | 1 cut(s) | 53 |
| SinI | GGWCC | 1 cut(s) | 374 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1107 |
| SmlI | CTYRAG | 1 cut(s) | 390 |
| SmoI | CTYRAG | 1 cut(s) | 390 |
| SsiI | CCGC | 7 cut(s) | 140, 178, 181, 212, 323, 1212, 1245 |
| SspI | AATATT | 1 cut(s) | 1452 |
| SspMI | CTAG | 2 cut(s) | 1281, 1947 |
| StyD4I | CCNGG | 4 cut(s) | 63, 221, 657, 1424 |
| StyI | CCWWGG | 3 cut(s) | 736, 856, 976 |
| TaaI | ACNGT | 3 cut(s) | 1196, 1349, 1398 |
| TaiI | ACGT | 6 cut(s) | 621, 727, 847, 967, 1087, 1904 |
| TaqI | TCGA | 4 cut(s) | 53, 81, 114, 1518 |
| TatI | WGTACW | 1 cut(s) | 1593 |
| TauI | GCSGC | 2 cut(s) | 183, 1248 |
| TfiI | GAWTC | 2 cut(s) | 97, 1502 |
| TscAI | CASTG | 2 cut(s) | 607, 1201 |
| TseFI | GTSAC | 1 cut(s) | 504 |
| TseI | GCWGC | 3 cut(s) | 193, 226, 278 |
| Tsp45I | GTSAC | 1 cut(s) | 504 |
| TspDTI | ATGAA | 3 cut(s) | 666, 1476, 1554 |
| TspGWI | ACGGA | 2 cut(s) | 1390, 1926 |
| TspRI | CASTG | 2 cut(s) | 607, 1201 |
| Van91I | CCANNNNNTGG | 4 cut(s) | 1157, 1214, 1639, 1663 |
| VpaK11BI | GGWCC | 1 cut(s) | 374 |
| VspI | ATTAAT | 2 cut(s) | 1167, 1482 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1947 |
| XapI | RAATTY | 6 cut(s) | 1310, 1373, 1492, 1813, 1876, 1891 |
| XbaI | TCTAGA | 1 cut(s) | 1946 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 824, 944, 1064 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1376 |
| XspI | CTAG | 2 cut(s) | 1281, 1947 |
| ZraI | GACGTC | 4 cut(s) | 725, 845, 965, 1085 |
| Zsp2I | ATGCAT | 1 cut(s) | 639 |