ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 588 |
| AccB1I | GGYRCC | 1 cut(s) | 478 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 309 |
| AccBSI | CCGCTC | 1 cut(s) | 2111 |
| AccI | GTMKAC | 1 cut(s) | 1786 |
| AciI | CCGC | 4 cut(s) | 149, 500, 2111, 2220 |
| AclWI | GGATC | 2 cut(s) | 603, 2422 |
| AcoI | YGGCCR | 1 cut(s) | 2244 |
| AcsI | RAATTY | 5 cut(s) | 683, 1074, 1260, 1501, 2449 |
| AcuI | CTGAAG | 4 cut(s) | 344, 1319, 1938, 2067 |
| AfaI | GTAC | 7 cut(s) | 44, 153, 768, 1843, 1935, 2194, 2400 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 309, 499, 802 |
| AgeI | ACCGGT | 1 cut(s) | 1633 |
| AjnI | CCWGG | 2 cut(s) | 1535, 1735 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 800, 832, 1151, 1183, 1487, 1519 |
| AleI | CACNNNNGTG | 1 cut(s) | 1512 |
| Alw21I | GWGCWC | 1 cut(s) | 847 |
| Alw26I | GTCTC | 2 cut(s) | 747, 2458 |
| AlwI | GGATC | 2 cut(s) | 603, 2422 |
| Ama87I | CYCGRG | 1 cut(s) | 1583 |
| AoxI | GGCC | 2 cut(s) | 793, 2244 |
| ApeKI | GCWGC | 8 cut(s) | 294, 297, 954, 1319, 1588, 1824, 2128, 2239 |
| ApoI | RAATTY | 5 cut(s) | 683, 1074, 1260, 1501, 2449 |
| AsiGI | ACCGGT | 1 cut(s) | 1633 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1608 |
| AspLEI | GCGC | 1 cut(s) | 2133 |
| AspS9I | GGNCC | 2 cut(s) | 220, 234 |
| AsuHPI | GGTGA | 5 cut(s) | 103, 785, 1007, 1183, 2227 |
| AsuNHI | GCTAGC | 1 cut(s) | 503 |
| AvaI | CYCGRG | 1 cut(s) | 1583 |
| AvaII | GGWCC | 2 cut(s) | 220, 234 |
| AxyI | CCTNAGG | 1 cut(s) | 514 |
| BaeGI | GKGCMC | 1 cut(s) | 1663 |
| BanI | GGYRCC | 1 cut(s) | 478 |
| BanII | GRGCYC | 1 cut(s) | 847 |
| BauI | CACGAG | 1 cut(s) | 1425 |
| Bbv12I | GWGCWC | 1 cut(s) | 847 |
| BbvI | GCAGC | 8 cut(s) | 281, 309, 941, 1331, 1575, 1811, 2140, 2226 |
| BccI | CCATC | 7 cut(s) | 355, 430, 1168, 1619, 1680, 2252, 2314 |
| BceAI | ACGGC | 2 cut(s) | 621, 2008 |
| BciT130I | CCWGG | 2 cut(s) | 1537, 1737 |
| BclI | TGATCA | 3 cut(s) | 1429, 2071, 2286 |
| BcoDI | GTCTC | 2 cut(s) | 747, 2458 |
| BfaI | CTAG | 2 cut(s) | 504, 791 |
| BfmI | CTRYAG | 4 cut(s) | 295, 1044, 1596, 1647 |
| BglII | AGATCT | 3 cut(s) | 106, 1204, 2269 |
| BisI | GCNGC | 9 cut(s) | 295, 298, 501, 955, 1320, 1589, 1825, 2129, 2240 |
| BlpI | GCTNAGC | 1 cut(s) | 486 |
| BlsI | GCNGC | 9 cut(s) | 296, 299, 502, 956, 1321, 1590, 1826, 2130, 2241 |
| Bme1390I | CCNGG | 2 cut(s) | 1537, 1737 |
| Bme18I | GGWCC | 2 cut(s) | 220, 234 |
| BmeT110I | CYCGRG | 1 cut(s) | 1583 |
| BmgT120I | GGNCC | 2 cut(s) | 220, 234 |
| BmiI | GGNNCC | 3 cut(s) | 366, 480, 1567 |
| BmrFI | CCNGG | 2 cut(s) | 1537, 1737 |
| BmsI | GCATC | 5 cut(s) | 136, 866, 1090, 1522, 1686 |
| BmtI | GCTAGC | 1 cut(s) | 507 |
| BpmI | CTGGAG | 2 cut(s) | 286, 1558 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 486 |
| BpuEI | CTTGAG | 4 cut(s) | 361, 1224, 1666, 2258 |
| BsaAI | YACGTR | 1 cut(s) | 1331 |
| BsaJI | CCNNGG | 2 cut(s) | 279, 1167 |
| BsaWI | WCCGGW | 2 cut(s) | 231, 1633 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 309, 499, 802 |
| Bse118I | RCCGGY | 3 cut(s) | 481, 1633, 2242 |
| Bse1I | ACTGG | 6 cut(s) | 218, 303, 858, 891, 1398, 2129 |
| Bse21I | CCTNAGG | 1 cut(s) | 514 |
| Bse3DI | GCAATG | 1 cut(s) | 103 |
| BseBI | CCWGG | 2 cut(s) | 1537, 1737 |
| BseDI | CCNNGG | 2 cut(s) | 279, 1167 |
| BseGI | GGATG | 4 cut(s) | 521, 1105, 1990, 2306 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 309, 499, 802 |
| BseMI | GCAATG | 1 cut(s) | 103 |
| BseMII | CTCAG | 6 cut(s) | 552, 758, 1691, 2190, 2339, 2453 |
| BseNI | ACTGG | 6 cut(s) | 218, 303, 858, 891, 1398, 2129 |
| BseRI | GAGGAG | 1 cut(s) | 499 |
| BseSI | GKGCMC | 1 cut(s) | 1663 |
| BseXI | GCAGC | 8 cut(s) | 281, 309, 941, 1331, 1575, 1811, 2140, 2226 |
| BseYI | CCCAGC | 1 cut(s) | 1995 |
| BshFI | GGCC | 2 cut(s) | 795, 2246 |
| BshNI | GGYRCC | 1 cut(s) | 478 |
| BshTI | ACCGGT | 1 cut(s) | 1633 |
| BsiHKAI | GWGCWC | 1 cut(s) | 847 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1583 |
| BsiSI | CCGG | 4 cut(s) | 232, 482, 1634, 2243 |
| BslFI | GGGAC | 3 cut(s) | 661, 1018, 1940 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 309, 499, 802 |
| BsmAI | GTCTC | 2 cut(s) | 747, 2458 |
| BsmFI | GGGAC | 3 cut(s) | 661, 1018, 1940 |
| BsmI | GAATGC | 2 cut(s) | 1720, 2010 |
| BsnI | GGCC | 2 cut(s) | 795, 2246 |
| BsoBI | CYCGRG | 1 cut(s) | 1583 |
| Bsp1286I | GDGCHC | 2 cut(s) | 847, 1663 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 486 |
| BspACI | CCGC | 4 cut(s) | 149, 500, 2111, 2220 |
| BspANI | GGCC | 2 cut(s) | 795, 2246 |
| BspCNI | CTCAG | 6 cut(s) | 553, 757, 1692, 2191, 2340, 2454 |
| BspLI | GGNNCC | 3 cut(s) | 366, 480, 1567 |
| BspMAI | CTGCAG | 1 cut(s) | 299 |
| BspOI | GCTAGC | 1 cut(s) | 507 |
| BspPI | GGATC | 2 cut(s) | 603, 2422 |
| BspT107I | GGYRCC | 1 cut(s) | 478 |
| BsrBI | CCGCTC | 1 cut(s) | 2111 |
| BsrDI | GCAATG | 1 cut(s) | 103 |
| BsrFI | RCCGGY | 3 cut(s) | 481, 1633, 2242 |
| BsrI | ACTGG | 6 cut(s) | 218, 303, 858, 891, 1398, 2129 |
| BssAI | RCCGGY | 3 cut(s) | 481, 1633, 2242 |
| BssECI | CCNNGG | 2 cut(s) | 279, 1167 |
| BssSI | CACGAG | 1 cut(s) | 1425 |
| BssT1I | CCWWGG | 2 cut(s) | 279, 1167 |
| Bst2BI | CACGAG | 1 cut(s) | 1425 |
| Bst2UI | CCWGG | 2 cut(s) | 1537, 1737 |
| Bst4CI | ACNGT | 6 cut(s) | 49, 69, 1651, 2165, 2211, 2430 |
| Bst6I | CTCTTC | 3 cut(s) | 265, 714, 2043 |
| BstBAI | YACGTR | 1 cut(s) | 1331 |
| BstC8I | GCNNGC | 4 cut(s) | 505, 1720, 1800, 2244 |
| BstEII | GGTNACC | 1 cut(s) | 2215 |
| BstF5I | GGATG | 4 cut(s) | 521, 1105, 1990, 2306 |
| BstHHI | GCGC | 1 cut(s) | 2133 |
| BstMAI | GTCTC | 2 cut(s) | 747, 2458 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 2128, 2239, 2380 |
| BstNI | CCWGG | 2 cut(s) | 1537, 1737 |
| BstNSI | RCATGY | 2 cut(s) | 294, 654 |
| BstPI | GGTNACC | 1 cut(s) | 2215 |
| BstSCI | CCNGG | 2 cut(s) | 1535, 1735 |
| BstSFI | CTRYAG | 4 cut(s) | 295, 1044, 1596, 1647 |
| BstSLI | GKGCMC | 1 cut(s) | 1663 |
| BstV1I | GCAGC | 8 cut(s) | 281, 309, 941, 1331, 1575, 1811, 2140, 2226 |
| BstX2I | RGATCY | 5 cut(s) | 106, 595, 1204, 2269, 2414 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1514 |
| BstYI | RGATCY | 5 cut(s) | 106, 595, 1204, 2269, 2414 |
| Bsu36I | CCTNAGG | 1 cut(s) | 514 |
| BsuRI | GGCC | 2 cut(s) | 795, 2246 |
| BtsCI | GGATG | 4 cut(s) | 521, 1105, 1990, 2306 |
| BtsIMutI | CAGTG | 6 cut(s) | 310, 548, 1103, 1697, 2122, 2161 |
| Cac8I | GCNNGC | 4 cut(s) | 505, 1720, 1800, 2244 |
| CfoI | GCGC | 1 cut(s) | 2133 |
| Cfr10I | RCCGGY | 3 cut(s) | 481, 1633, 2242 |
| Cfr13I | GGNCC | 2 cut(s) | 220, 234 |
| CseI | GACGC | 1 cut(s) | 554 |
| Csp6I | GTAC | 7 cut(s) | 43, 152, 767, 1842, 1934, 2193, 2399 |
| CspAI | ACCGGT | 1 cut(s) | 1633 |
| CviQI | GTAC | 7 cut(s) | 43, 152, 767, 1842, 1934, 2193, 2399 |
| DraI | TTTAAA | 1 cut(s) | 1897 |
| EaeI | YGGCCR | 1 cut(s) | 2244 |
| Eam1104I | CTCTTC | 3 cut(s) | 265, 714, 2043 |
| EarI | CTCTTC | 3 cut(s) | 265, 714, 2043 |
| Ecl136II | GAGCTC | 1 cut(s) | 845 |
| Eco130I | CCWWGG | 2 cut(s) | 279, 1167 |
| Eco147I | AGGCCT | 1 cut(s) | 795 |
| Eco24I | GRGCYC | 1 cut(s) | 847 |
| Eco32I | GATATC | 2 cut(s) | 943, 1474 |
| Eco47I | GGWCC | 2 cut(s) | 220, 234 |
| Eco53kI | GAGCTC | 1 cut(s) | 845 |
| Eco57I | CTGAAG | 4 cut(s) | 344, 1319, 1938, 2067 |
| Eco81I | CCTNAGG | 1 cut(s) | 514 |
| Eco88I | CYCGRG | 1 cut(s) | 1583 |
| Eco91I | GGTNACC | 1 cut(s) | 2215 |
| EcoICRI | GAGCTC | 1 cut(s) | 845 |
| EcoO65I | GGTNACC | 1 cut(s) | 2215 |
| EcoRI | GAATTC | 1 cut(s) | 683 |
| EcoRII | CCWGG | 2 cut(s) | 1535, 1735 |
| EcoRV | GATATC | 2 cut(s) | 943, 1474 |
| EcoT14I | CCWWGG | 2 cut(s) | 279, 1167 |
| EcoT22I | ATGCAT | 1 cut(s) | 2010 |
| EcoT38I | GRGCYC | 1 cut(s) | 847 |
| ErhI | CCWWGG | 2 cut(s) | 279, 1167 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 584, 616, 1702, 1734 |
| FaqI | GGGAC | 3 cut(s) | 661, 1018, 1940 |
| FbaI | TGATCA | 3 cut(s) | 1429, 2071, 2286 |
| FblI | GTMKAC | 1 cut(s) | 1786 |
| Fnu4HI | GCNGC | 9 cut(s) | 295, 298, 501, 955, 1320, 1589, 1825, 2129, 2240 |
| FokI | GGATG | 4 cut(s) | 508, 1112, 1997, 2293 |
| FriOI | GRGCYC | 1 cut(s) | 847 |
| Fsp4HI | GCNGC | 9 cut(s) | 295, 298, 501, 955, 1320, 1589, 1825, 2129, 2240 |
| FspBI | CTAG | 2 cut(s) | 504, 791 |
| GlaI | GCGC | 1 cut(s) | 2132 |
| GluI | GCNGC | 9 cut(s) | 295, 298, 501, 955, 1320, 1589, 1825, 2129, 2240 |
| GsaI | CCCAGC | 1 cut(s) | 1999 |
| GsuI | CTGGAG | 2 cut(s) | 286, 1558 |
| HaeIII | GGCC | 2 cut(s) | 795, 2246 |
| HapII | CCGG | 4 cut(s) | 232, 482, 1634, 2243 |
| HgaI | GACGC | 1 cut(s) | 554 |
| HhaI | GCGC | 1 cut(s) | 2133 |
| Hin6I | GCGC | 1 cut(s) | 2131 |
| HinP1I | GCGC | 1 cut(s) | 2131 |
| HincII | GTYRAC | 1 cut(s) | 1948 |
| HindII | GTYRAC | 1 cut(s) | 1948 |
| HpaII | CCGG | 4 cut(s) | 232, 482, 1634, 2243 |
| HphI | GGTGA | 5 cut(s) | 103, 785, 1007, 1183, 2227 |
| Hpy166II | GTNNAC | 7 cut(s) | 45, 229, 1236, 1271, 1445, 1787, 1948 |
| Hpy8I | GTNNAC | 7 cut(s) | 45, 229, 1236, 1271, 1445, 1787, 1948 |
| HpyAV | CCTTC | 8 cut(s) | 112, 161, 319, 386, 1294, 1546, 1913, 2321 |
| HpyCH4III | ACNGT | 6 cut(s) | 49, 69, 1651, 2165, 2211, 2430 |
| HpyCH4IV | ACGT | 3 cut(s) | 204, 1330, 1731 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 2128, 2239, 2380 |
| HpySE526I | ACGT | 3 cut(s) | 204, 1330, 1731 |
| HspAI | GCGC | 1 cut(s) | 2131 |
| KroI | GCCGGC | 1 cut(s) | 2242 |
| KroNI | GCCGGC | 1 cut(s) | 2244 |
| Ksp22I | TGATCA | 3 cut(s) | 1429, 2071, 2286 |
| LmnI | GCTCC | 7 cut(s) | 170, 305, 370, 512, 1011, 1294, 2108 |
| Lsp1109I | GCAGC | 8 cut(s) | 281, 309, 941, 1331, 1575, 1811, 2140, 2226 |
| LweI | GCATC | 5 cut(s) | 136, 866, 1090, 1522, 1686 |
| MaeI | CTAG | 2 cut(s) | 504, 791 |
| MaeII | ACGT | 3 cut(s) | 204, 1330, 1731 |
| MaeIII | GTNAC | 4 cut(s) | 121, 1326, 1458, 2215 |
| MbiI | CCGCTC | 1 cut(s) | 2111 |
| MfeI | CAATTG | 1 cut(s) | 921 |
| MflI | RGATCY | 5 cut(s) | 106, 595, 1204, 2269, 2414 |
| MhlI | GDGCHC | 2 cut(s) | 847, 1663 |
| MlyI | GAGTC | 3 cut(s) | 1000, 1973, 2460 |
| MmeI | TCCRAC | 5 cut(s) | 7, 198, 549, 1146, 1633 |
| Mph1103I | ATGCAT | 1 cut(s) | 2010 |
| MroNI | GCCGGC | 1 cut(s) | 2242 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1608 |
| MseI | TTAA | 8 cut(s) | 1028, 1548, 1614, 1896, 2082, 2282, 2321, 2408 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1484, 1512, 1957, 2183 |
| MspI | CCGG | 4 cut(s) | 232, 482, 1634, 2243 |
| MspR9I | CCNGG | 2 cut(s) | 1537, 1737 |
| MunI | CAATTG | 1 cut(s) | 921 |
| Mva1269I | GAATGC | 2 cut(s) | 1720, 2010 |
| MvaI | CCWGG | 2 cut(s) | 1537, 1737 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 2128, 2239, 2380 |
| NaeI | GCCGGC | 1 cut(s) | 2244 |
| NgoMIV | GCCGGC | 1 cut(s) | 2242 |
| NheI | GCTAGC | 1 cut(s) | 503 |
| NlaIV | GGNNCC | 3 cut(s) | 366, 480, 1567 |
| NmuCI | GTSAC | 2 cut(s) | 1458, 2215 |
| NsiI | ATGCAT | 1 cut(s) | 2010 |
| NspI | RCATGY | 2 cut(s) | 294, 654 |
| OliI | CACNNNNGTG | 1 cut(s) | 1512 |
| PaeR7I | CTCGAG | 1 cut(s) | 1583 |
| PceI | AGGCCT | 1 cut(s) | 795 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1349 |
| PctI | GAATGC | 2 cut(s) | 1720, 2010 |
| PdiI | GCCGGC | 1 cut(s) | 2244 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1608 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 309 |
| PinAI | ACCGGT | 1 cut(s) | 1633 |
| PkrI | GCNGC | 9 cut(s) | 296, 299, 502, 956, 1321, 1590, 1826, 2130, 2241 |
| PleI | GAGTC | 3 cut(s) | 1000, 1973, 2460 |
| PpsI | GAGTC | 3 cut(s) | 1000, 1973, 2460 |
| Ppu21I | YACGTR | 1 cut(s) | 1331 |
| PsiI | TTATAA | 1 cut(s) | 588 |
| Psp124BI | GAGCTC | 1 cut(s) | 847 |
| Psp6I | CCWGG | 2 cut(s) | 1535, 1735 |
| PspEI | GGTNACC | 1 cut(s) | 2215 |
| PspFI | CCCAGC | 1 cut(s) | 1995 |
| PspGI | CCWGG | 2 cut(s) | 1535, 1735 |
| PspN4I | GGNNCC | 3 cut(s) | 366, 480, 1567 |
| PspPI | GGNCC | 2 cut(s) | 220, 234 |
| PstI | CTGCAG | 1 cut(s) | 299 |
| PsuI | RGATCY | 5 cut(s) | 106, 595, 1204, 2269, 2414 |
| RsaI | GTAC | 7 cut(s) | 44, 153, 768, 1843, 1935, 2194, 2400 |
| RsaNI | GTAC | 7 cut(s) | 43, 152, 767, 1842, 1934, 2193, 2399 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1484, 1512, 1957, 2183 |
| SacI | GAGCTC | 1 cut(s) | 847 |
| SaqAI | TTAA | 8 cut(s) | 1028, 1548, 1614, 1896, 2082, 2282, 2321, 2408 |
| SatI | GCNGC | 9 cut(s) | 295, 298, 501, 955, 1320, 1589, 1825, 2129, 2240 |
| Sau96I | GGNCC | 2 cut(s) | 220, 234 |
| SchI | GAGTC | 3 cut(s) | 1000, 1973, 2460 |
| ScrFI | CCNGG | 2 cut(s) | 1537, 1737 |
| SduI | GDGCHC | 2 cut(s) | 847, 1663 |
| SfaNI | GCATC | 5 cut(s) | 136, 866, 1090, 1522, 1686 |
| SfcI | CTRYAG | 4 cut(s) | 295, 1044, 1596, 1647 |
| Sfr274I | CTCGAG | 1 cut(s) | 1583 |
| SinI | GGWCC | 2 cut(s) | 220, 234 |
| SlaI | CTCGAG | 1 cut(s) | 1583 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1484, 1512, 1957, 2183 |
| SmlI | CTYRAG | 5 cut(s) | 340, 1239, 1583, 1681, 2273 |
| SmoI | CTYRAG | 5 cut(s) | 340, 1239, 1583, 1681, 2273 |
| SseBI | AGGCCT | 1 cut(s) | 795 |
| SsiI | CCGC | 4 cut(s) | 149, 500, 2111, 2220 |
| SspI | AATATT | 1 cut(s) | 1371 |
| SspMI | CTAG | 2 cut(s) | 504, 791 |
| SstI | GAGCTC | 1 cut(s) | 847 |
| StuI | AGGCCT | 1 cut(s) | 795 |
| StyD4I | CCNGG | 2 cut(s) | 1535, 1735 |
| StyI | CCWWGG | 2 cut(s) | 279, 1167 |
| TaaI | ACNGT | 6 cut(s) | 49, 69, 1651, 2165, 2211, 2430 |
| TaiI | ACGT | 3 cut(s) | 207, 1333, 1734 |
| TaqI | TCGA | 4 cut(s) | 1584, 2256, 2403, 2424 |
| TaqII | GACCGA | 1 cut(s) | 237 |
| TatI | WGTACW | 4 cut(s) | 42, 766, 1841, 2192 |
| TauI | GCSGC | 1 cut(s) | 503 |
| Tru1I | TTAA | 8 cut(s) | 1028, 1548, 1614, 1896, 2082, 2282, 2321, 2408 |
| Tru9I | TTAA | 8 cut(s) | 1028, 1548, 1614, 1896, 2082, 2282, 2321, 2408 |
| TscAI | CASTG | 6 cut(s) | 310, 555, 1110, 1704, 2129, 2168 |
| TseFI | GTSAC | 2 cut(s) | 1458, 2215 |
| TseI | GCWGC | 8 cut(s) | 294, 297, 954, 1319, 1588, 1824, 2128, 2239 |
| Tsp45I | GTSAC | 2 cut(s) | 1458, 2215 |
| TspDTI | ATGAA | 9 cut(s) | 362, 510, 676, 713, 1131, 1502, 1632, 1794, 2078 |
| TspGWI | ACGGA | 2 cut(s) | 2165, 2401 |
| TspRI | CASTG | 6 cut(s) | 310, 555, 1110, 1704, 2129, 2168 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 309 |
| VpaK11BI | GGWCC | 2 cut(s) | 220, 234 |
| XapI | RAATTY | 5 cut(s) | 683, 1074, 1260, 1501, 2449 |
| XceI | RCATGY | 2 cut(s) | 294, 654 |
| XhoI | CTCGAG | 1 cut(s) | 1583 |
| XmiI | GTMKAC | 1 cut(s) | 1786 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1608 |
| XspI | CTAG | 2 cut(s) | 504, 791 |
| Zsp2I | ATGCAT | 1 cut(s) | 2010 |