Rh6CG461200

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000203
Physical Location & Seq
Reverse (-)
63503238 .. 63509656
6419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG461200.1

Sequence Viewer

Length: 3591 bp
ATGGACATTATTCTAGCATTGGTACAACCATTGGCAGAATGGGCAGTGAGACCAATTCGGCGACAGTTGAGTTATGTGTTTCACTACAAGAGCAACATTGAGCATCTCAGCGAACAGGTTCTGCTGCTGCGTGACAAAAGAGGAGGAGTAAACCTGGACGTGACAGATGCGAGACGAGCTCTACAGGAGATTAGTCCCGGAGTTGATCGCTGGCTCGGTGATGTAGACAAGATCATAGCAGAAGAGGAAACGAGTTTCAATGAAGAAACTGTAGCAGCAAAGGCAGCGTGCTGTAATGGGTGGTTGCCAAATTTGAAGTGTCGTCATTCTTTGGGAAGGAAAGCTAAGAAGATGACTGAGAAGGTGGATAATCTTCTTGCTAATGAACCCAGGGCTATAGCGCATCCTGCTGCTCCCCCAAAGGTGGAATTCAAACCCACAGAGCACTCTACTCTGCCCGGAACCAACTTCCATGAAGGTGCCAGCTCCTCAGGAACATCAACCCAAGAACAGCCACCTGCATCTGAAGGATATAATATTAATTTTGATTCAAGAAGATCAACCATTAGAGATGTGATGGAAGCTCTCAAGGGGAATCAATTCAATCCGATTATCATATGCGGCATGGGCGGCATCGGGAAGACGACATTGATGGGGCAAGTTTTTGAGAAAGCAAAGGAAGAAGGTCTGTTCGATGAGTATACAAAGGCAACCTTATCTCTTGAAAAGGCTCCTGATGTGATTCAGATTCAAGATGAACTTGCTCGATGTCTAGGCCTACCACTTGAAGGAAAAGAGAGTGGTCTAAGAGCAAGTAAGCTACGTGAAAGATTGTCTCAAGGCAGTAAGAAGATCCTTGTAATGTTAGACAATGTTTCCACAACAACCCCAGACTTTTTGTGGGACATAGGGATTCCCCTTAGTTGTAAACTTTTGGTGACATCAAGACAACAAGATCTATTCAAGGACATGGACACAAGAAAGAATTTCCCCATTCATGGTTTGCCGGATACTGATGCATGGAGTCTGTTTAAGAAGACAGCAGGAATTTCTATCGAATCTGATCGCGAGTTAGAAGTTGTAGCAAAAGAGGTTTTGAAGGAATGTGCTGGGTTGCCCATTGCAATTTCAACTGTTGGAACGGCACTAAAAGGTGAAAACATTGCAGTTTGGACGAATGCACTAAGGGAACTGAAGAAGGCTAGTCCAGAAAACGTTCCAGGAGTGATAGAACATGTGTATGGCAAAATCAAGTTCAGCTATAAATGTTTACCAAGCGAGCAAGCCAAATCATGCTTTTTGTTGTGTTGCATGTTTGAAGAAAGCGCTTATATAGAGATAGAAGATTTGGTTAGATATGGGCTCGGTCTCAAGCTCTTTAAAGGCATTGATTCAATGGTTGAAGGAAGAAACTGTATAGAAACATTGGTTCAGACACTCAAATATCGCTTTTTATTGTTAGAGAGCGATGAGCCAGAGTGTGTGAGAATGCATGATGTGGTGCGTGATGTTGCCGTGCATATAGCTTCTGAAGAGTTTGTAGAAAAGAAAAATGATGGGGTTGAAAAGAGAATTGTGGTGAGAGACAAAGCAGAAACTGTGAAGTTGATCGATTGGCATAAGGATGGTGATTCTAAGCGATCTATTTATTGCAGTTCACTGAGTATCAATGGACTTTGTCAACTTTTACTGTTGAGAAATCTATCTGGATTTCAAATATTGGAGCCTCCACCAACAATTTTCAATAGAATGGAAGATCTCAAGGTTCTAGCAATAAATGAGAAGGACACGAGGTTTCAAGGTATCAAAATGCATATGTTGTCACTAGTGTCATCACTTGCCGTTTTGAAAAACCTTCAAACACTCTGTCTAGAGGGGTGCGATCTGGAGTGTGATGTATCTGTCATTGGGGAGCTAAGGACACTCATGATACTCAGTTTGCGTGAATCCAACATCAAGCAATTGCCTGATACATTTAAAAATTTGTCTAATCTGAGATTGCTTGATTTGTCCGAATGTACGGAGCTTCAAATAATCTCACCAAGTGTGATATCAAGTTTATCCCCACTAGAAGAATTGTACATGTTGGACAGCTTTGCTAATTGGGTGATTGATAAACTAGCCTCGATCGAAACAACCAATTGGATCTCTCAATTGGAGGCTGAAGAAAGAGCTGGATCTGAAGAAGTGAACAAGTGGAAGAGGATCAACACATTGGCGGTTGAACAACCAGCCAACATAAGGGAGATTGATGGGCTTCTTGAAGAATGTGAAGGGAGGAGAAGGTGGGCCAAGTCCATGGACTGGACAAGTGGGATACTTTCAGAACTACTTTCCTTGTCCGGTTTGACTCGTTTAAGTGTTTTGCTACCACCGCTTGATATTCTGCTAACAACCGATGTACTGGGCAAGAAGCTTGAAAGATTTCAGATCTCTTTAGGATGGACTAATCCATGGATACTTTTTGTCCCGCCTTCTGAGAACTACTTGAGAGTTTTTGATCTTGATGCAAGCTCACTCGTGGGTAGTAATATCAGTTGGTTGCTAAACAAAACTAACACTCTTGTGTTACGCATGAAGAATTTGACAGATCCTCTGAATGTCTTCGATTTTGCAAACTTGAAGAGACTTGAACTCAAGGACTGTGATGCTCTGGAATATCTGATTGACGTGACACGTGTAAACCCTGCTACTCCACGCGGTACCGTCTTTCCCGTCTTGGTGTCACTGGAAGTCAGTGGTGCAACAAGGCTGAAGGAGATTTGTCATGGTGAGCTGCTCCACACCAAGGTTCATTTTCCAGTCTTTAGTGATGACAACATCTTCCCTCAGCTAACAAGTTTGAAGCTTGTTAATCTTCCAAATCTCAGAACTCTGCTCTCTCCTTCAATCGCCAGAGGTCTTGTGAAGCTCAAAAGACTAAGGATCGTTGGCTGCGAGAAGATTGAAGAGATAGTAGCAGCAGCAGAGGGTGATGAAGAAACAGAGGATGACAACATCTTCCCTCAGCTAACATATTTGCAGCTTGAGGATCTACCCAATCTCAGAACTCTGCTCTCCCCTTCAATCGCCAGAGGTCTTCTGAAGCTCGAAACACTATGGATCGAAAACTGCGAGAAGATTGAAGAGATAGTAGCAGCAGCAGAGGGTGATGAAGAAACAGAGGATGACAACATCTTCCCTCAGCTAGCACATTTGTATCTTATTAATCTTCCCAATCTCAGAACTCTGCTCTCCCCTTCAATCGCCAGAGGTCTTGTGAAGCTCGAAACACTAAGGATCTTTGACTGCGAGAAGATTGAAGAGATAGTAGCAGCAGCAGAGGGTGATGAAGAAACAGAGGATGACAACATCTTCCCTCAGCTAACATATTTGTATCTTATTAATCTTCCAAATCTCGGAAGCTTTTCGCAAGGCAAATATAATTTCAAATGGCCACTAGCGAAAAACATAGAAATCGAAGGATGCTGCAATATGAAGAAGTTTTGTTTCGGATCCGTCAGCATACCAAGGGAAGTGGAATTGGACGTCAGGGATGATGTGGGCGAGAATGTTTTGGAGGAGCTGAATAACAGTAGAAAAGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1196

Amino Acids

134.43

Weight (kDa)

5.11

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 187 - 348 6.3e-19 NB-ARC domain
LRR_14 PF23598 608 - 743 1.6e-07 Leucine-rich repeat region
LRR_8 PF13855 640 - 695 1.5e-06 Leucine rich repeat
LRR_RPS2 PF23247 871 - 980 5e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 918 - 1019 2.1e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 996 - 1077 9.1e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 526
AatII GACGTC 1 cut(s) 3531
Acc36I ACCTGC 1 cut(s) 526
Acc65I GGTACC 1 cut(s) 2702
AccB1I GGYRCC 2 cut(s) 479, 2702
AccB7I CCANNNNNTGG 1 cut(s) 2304
AccI GTMKAC 2 cut(s) 225, 701
AccII CGCG 2 cut(s) 1068, 2700
AciI CCGC 6 cut(s) 621, 630, 2219, 2375, 2471, 2700
AclI AACGTT 1 cut(s) 1215
AcoI YGGCCR 1 cut(s) 3434
AcsI RAATTY 6 cut(s) 310, 428, 985, 1047, 1981, 2581
AcuI CTGAAG 7 cut(s) 546, 1214, 1551, 2184, 2202, 2774, 3104
AcvI CACGTG 1 cut(s) 2678
AcyI GRCGYC 1 cut(s) 3528
AdeI CACNNNGTG 1 cut(s) 2045
AfaI GTAC 5 cut(s) 24, 2020, 2081, 2403, 2704
AfeI AGCGCT 1 cut(s) 1327
AfiI CCNNNNNNNGG 7 cut(s) 424, 788, 998, 2241, 2304, 2788, 3398
AflIII ACRYGT 4 cut(s) 1234, 2082, 2675, 2677
AhlI ACTAGT 1 cut(s) 1825
AjiI CACGTC 2 cut(s) 160, 2671
AjnI CCWGG 3 cut(s) 153, 389, 1219
AleI CACNNNNGTG 1 cut(s) 2564
Alw21I GWGCWC 2 cut(s) 181, 447
Alw26I GTCTC 6 cut(s) 43, 166, 840, 1373, 1578, 2620
AlwNI CAGNNNCTG 2 cut(s) 121, 1598
Aor51HI AGCGCT 1 cut(s) 1327
AoxI GGCC 3 cut(s) 775, 2289, 3434
ApoI RAATTY 6 cut(s) 310, 428, 985, 1047, 1981, 2581
ArsI GACNNNNNNTTYG 2 cut(s) 1852, 1884
AseI ATTAAT 3 cut(s) 540, 3207, 3384
Asp700I GAANNNNTTC 6 cut(s) 117, 599, 1215, 2424, 2603, 3406
Asp718I GGTACC 1 cut(s) 2702
AspLEI GCGC 2 cut(s) 403, 1328
AspS9I GGNCC 1 cut(s) 2289
AsuC2I CCSGG 2 cut(s) 198, 459
AsuNHI GCTAGC 1 cut(s) 3187
AxyI CCTNAGG 1 cut(s) 490
BaeI ACNNNNGTAYC 2 cut(s) 3182, 3215
BalI TGGCCA 1 cut(s) 3436
BamHI GGATCC 1 cut(s) 3494
BanI GGYRCC 2 cut(s) 479, 2702
BanII GRGCYC 2 cut(s) 181, 1365
BauI CACGAG 2 cut(s) 1789, 2519
BbrPI CACGTG 1 cut(s) 2678
BbsI GAAGAC 4 cut(s) 647, 1043, 2596, 3071
Bbv12I GWGCWC 2 cut(s) 181, 447
BbvCI CCTCAGC 4 cut(s) 2829, 3006, 3183, 3360
BccI CCATC 6 cut(s) 571, 646, 1550, 1619, 2246, 2436
BceAI ACGGC 3 cut(s) 1158, 1499, 1826
BciT130I CCWGG 3 cut(s) 155, 391, 1221
BciVI GTATCC 3 cut(s) 1003, 2310, 2451
BcnI CCSGG 2 cut(s) 198, 459
BcoDI GTCTC 6 cut(s) 43, 166, 840, 1373, 1578, 2620
BcuI ACTAGT 1 cut(s) 1825
BfmI CTRYAG 3 cut(s) 182, 270, 396
BfoI RGCGCY 1 cut(s) 1329
BfuAI ACCTGC 1 cut(s) 526
BfuI GTATCC 3 cut(s) 1003, 2310, 2451
BglII AGATCT 3 cut(s) 955, 1756, 2430
Bme1390I CCNGG 5 cut(s) 155, 198, 391, 459, 1221
BmgBI CACGTC 2 cut(s) 160, 2671
BmgT120I GGNCC 1 cut(s) 2289
BmiI GGNNCC 6 cut(s) 463, 481, 732, 1725, 2704, 3496
BmrFI CCNGG 5 cut(s) 155, 198, 391, 459, 1221
BmrI ACTGGG 1 cut(s) 2414
BmsI GCATC 9 cut(s) 112, 157, 412, 530, 642, 1006, 2497, 2638, 3455
BmtI GCTAGC 1 cut(s) 3191
BmuI ACTGGG 1 cut(s) 2414
BpiI GAAGAC 4 cut(s) 647, 1043, 2596, 3071
BplI GAGNNNNNCTC 2 cut(s) 163, 195
BpmI CTGGAG 1 cut(s) 1907
Bpu10I CCTNAGC 5 cut(s) 1916, 2829, 3006, 3183, 3360
BpuEI CTTGAG 7 cut(s) 572, 822, 1355, 1745, 2509, 2621, 3047
BpuMI CCSGG 2 cut(s) 198, 459
Bsa29I ATCGAT 1 cut(s) 1611
BsaAI YACGTR 2 cut(s) 824, 2678
BsaHI GRCGYC 1 cut(s) 3528
BsaI GGTCTC 2 cut(s) 43, 1373
BsaJI CCNNGG 6 cut(s) 389, 390, 2298, 2453, 2787, 3509
BsaWI WCCGGW 1 cut(s) 2342
Bsc4I CCNNNNNNNGG 7 cut(s) 424, 788, 998, 2241, 2304, 2788, 3398
Bse1I ACTGG 4 cut(s) 2309, 2409, 2733, 2801
Bse21I CCTNAGG 1 cut(s) 490
Bse3DI GCAATG 2 cut(s) 1119, 1161
BseBI CCWGG 3 cut(s) 155, 391, 1221
BseCI ATCGAT 1 cut(s) 1611
BseDI CCNNGG 6 cut(s) 389, 390, 2298, 2453, 2787, 3509
BseGI GGATG 8 cut(s) 403, 1630, 2447, 2995, 3172, 3349, 3470, 3541
BseLI CCNNNNNNNGG 7 cut(s) 424, 788, 998, 2241, 2304, 2788, 3398
BseMI GCAATG 2 cut(s) 1119, 1161
BseNI ACTGG 4 cut(s) 2309, 2409, 2733, 2801
BseRI GAGGAG 5 cut(s) 156, 159, 478, 2293, 3575
BseYI CCCAGC 1 cut(s) 1109
Bsh1236I CGCG 2 cut(s) 1068, 2700
Bsh1285I CGRYCG 1 cut(s) 2130
BshFI GGCC 3 cut(s) 777, 2291, 3436
BshNI GGYRCC 2 cut(s) 479, 2702
BshVI ATCGAT 1 cut(s) 1611
BsiEI CGRYCG 1 cut(s) 2130
BsiHKAI GWGCWC 2 cut(s) 181, 447
BsiSI CCGG 4 cut(s) 198, 459, 1007, 2343
BslFI GGGAC 3 cut(s) 180, 917, 2453
BslI CCNNNNNNNGG 7 cut(s) 424, 788, 998, 2241, 2304, 2788, 3398
BsmAI GTCTC 6 cut(s) 43, 166, 840, 1373, 1578, 2620
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 3 cut(s) 180, 917, 2453
BsmI GAATGC 2 cut(s) 1183, 1494
BsnI GGCC 3 cut(s) 777, 2291, 3436
Bso31I GGTCTC 2 cut(s) 43, 1373
Bsp1286I GDGCHC 3 cut(s) 181, 447, 1365
Bsp1407I TGTACA 1 cut(s) 2079
Bsp19I CCATGG 2 cut(s) 2298, 2453
Bsp68I TCGCGA 1 cut(s) 1068
BspACI CCGC 6 cut(s) 621, 630, 2219, 2375, 2471, 2700
BspANI GGCC 3 cut(s) 777, 2291, 3436
BspDI ATCGAT 1 cut(s) 1611
BspFNI CGCG 2 cut(s) 1068, 2700
BspHI TCATGA 1 cut(s) 1926
BspLI GGNNCC 6 cut(s) 463, 481, 732, 1725, 2704, 3496
BspMI ACCTGC 1 cut(s) 526
BspOI GCTAGC 1 cut(s) 3191
BspT107I GGYRCC 2 cut(s) 479, 2702
BspTNI GGTCTC 2 cut(s) 43, 1373
BsrDI GCAATG 2 cut(s) 1119, 1161
BsrGI TGTACA 1 cut(s) 2079
BsrI ACTGG 4 cut(s) 2309, 2409, 2733, 2801
BssECI CCNNGG 6 cut(s) 389, 390, 2298, 2453, 2787, 3509
BssNAI GTATAC 1 cut(s) 702
BssNI GRCGYC 1 cut(s) 3528
BssSI CACGAG 2 cut(s) 1789, 2519
BssT1I CCWWGG 4 cut(s) 2298, 2453, 2787, 3509
Bst1107I GTATAC 1 cut(s) 702
Bst2BI CACGAG 2 cut(s) 1789, 2519
Bst2UI CCWGG 3 cut(s) 155, 391, 1221
Bst4CI ACNGT 9 cut(s) 66, 271, 1135, 1415, 1600, 1692, 2645, 2707, 3575
Bst6I CTCTTC 7 cut(s) 237, 1527, 2195, 2618, 2943, 3120, 3297
BstACI GRCGYC 1 cut(s) 3528
BstAUI TGTACA 1 cut(s) 2079
BstBAI YACGTR 2 cut(s) 824, 2678
BstC8I GCNNGC 7 cut(s) 212, 289, 484, 1280, 1284, 2512, 3189
BstDSI CCRYGG 2 cut(s) 2298, 2453
BstF5I GGATG 8 cut(s) 403, 1630, 2447, 2995, 3172, 3349, 3470, 3541
BstFNI CGCG 2 cut(s) 1068, 2700
BstH2I RGCGCY 1 cut(s) 1329
BstHHI GCGC 2 cut(s) 403, 1328
BstMAI GTCTC 6 cut(s) 43, 166, 840, 1373, 1578, 2620
BstMCI CGRYCG 1 cut(s) 2130
BstMWI GCNNNNNNNGC 8 cut(s) 41, 176, 281, 284, 407, 627, 630, 2374
BstNI CCWGG 3 cut(s) 155, 391, 1221
BstNSI RCATGY 3 cut(s) 1238, 1315, 2086
BstSCI CCNGG 5 cut(s) 153, 196, 389, 457, 1219
BstSFI CTRYAG 3 cut(s) 182, 270, 396
BstUI CGCG 2 cut(s) 1068, 2700
BstV2I GAAGAC 4 cut(s) 647, 1043, 2596, 3071
BstXI CCANNNNNNTGG 1 cut(s) 2299
BstZ17I GTATAC 1 cut(s) 702
Bsu15I ATCGAT 1 cut(s) 1611
Bsu36I CCTNAGG 1 cut(s) 490
BsuI GTATCC 3 cut(s) 1003, 2310, 2451
BsuRI GGCC 3 cut(s) 777, 2291, 3436
BsuTUI ATCGAT 1 cut(s) 1611
BtgI CCRYGG 2 cut(s) 2298, 2453
BtgZI GCGATG 1 cut(s) 1482
BtrI CACGTC 2 cut(s) 160, 2671
BtsCI GGATG 8 cut(s) 403, 1630, 2447, 2995, 3172, 3349, 3470, 3541
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 4 cut(s) 51, 1658, 2726, 2743
BtuMI TCGCGA 1 cut(s) 1068
BveI ACCTGC 1 cut(s) 526
Cac8I GCNNGC 7 cut(s) 212, 289, 484, 1280, 1284, 2512, 3189
CaiI CAGNNNCTG 2 cut(s) 121, 1598
CciI TCATGA 1 cut(s) 1926
CfoI GCGC 2 cut(s) 403, 1328
Cfr13I GGNCC 1 cut(s) 2289
ClaI ATCGAT 1 cut(s) 1611
Csp6I GTAC 5 cut(s) 23, 2019, 2080, 2402, 2703
CspCI CAANNNNNGTGG 2 cut(s) 3498, 3533
CviQI GTAC 5 cut(s) 23, 2019, 2080, 2402, 2703
DraI TTTAAA 2 cut(s) 1381, 1978
DraIII CACNNNGTG 1 cut(s) 2045
EaeI YGGCCR 1 cut(s) 3434
Eam1104I CTCTTC 7 cut(s) 237, 1527, 2195, 2618, 2943, 3120, 3297
EarI CTCTTC 7 cut(s) 237, 1527, 2195, 2618, 2943, 3120, 3297
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 4 cut(s) 2298, 2453, 2787, 3509
Eco147I AGGCCT 1 cut(s) 777
Eco24I GRGCYC 2 cut(s) 181, 1365
Eco31I GGTCTC 2 cut(s) 43, 1373
Eco32I GATATC 1 cut(s) 2052
Eco47III AGCGCT 1 cut(s) 1327
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 7 cut(s) 546, 1214, 1551, 2184, 2202, 2774, 3104
Eco72I CACGTG 1 cut(s) 2678
Eco81I CCTNAGG 1 cut(s) 490
EcoICRI GAGCTC 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 3 cut(s) 153, 389, 1219
EcoRV GATATC 1 cut(s) 2052
EcoT14I CCWWGG 4 cut(s) 2298, 2453, 2787, 3509
EcoT22I ATGCAT 3 cut(s) 1021, 1494, 1815
EcoT38I GRGCYC 2 cut(s) 181, 1365
ErhI CCWWGG 4 cut(s) 2298, 2453, 2787, 3509
Esp3I CGTCTC 1 cut(s) 166
FalI AAGNNNNNCTT 4 cut(s) 698, 730, 744, 776
FaqI GGGAC 3 cut(s) 180, 917, 2453
FauI CCCGC 1 cut(s) 2478
FauNDI CATATG 2 cut(s) 617, 1815
FblI GTMKAC 2 cut(s) 225, 701
FokI GGATG 8 cut(s) 390, 1637, 2454, 3002, 3179, 3356, 3477, 3548
FriOI GRGCYC 2 cut(s) 181, 1365
GlaI GCGC 2 cut(s) 402, 1327
GsaI CCCAGC 1 cut(s) 1113
GsuI CTGGAG 1 cut(s) 1907
HaeII RGCGCY 1 cut(s) 1329
HaeIII GGCC 3 cut(s) 777, 2291, 3436
HapII CCGG 4 cut(s) 198, 459, 1007, 2343
HhaI GCGC 2 cut(s) 403, 1328
Hin1I GRCGYC 1 cut(s) 3528
Hin6I GCGC 2 cut(s) 401, 1326
HinP1I GCGC 2 cut(s) 401, 1326
HincII GTYRAC 1 cut(s) 1682
HindII GTYRAC 1 cut(s) 1682
HindIII AAGCTT 3 cut(s) 2414, 2846, 3403
HpaII CCGG 4 cut(s) 198, 459, 1007, 2343
Hpy166II GTNNAC 9 cut(s) 151, 226, 702, 929, 1271, 1658, 1682, 2191, 2683
Hpy8I GTNNAC 9 cut(s) 151, 226, 702, 929, 1271, 1658, 1682, 2191, 2683
HpyCH4III ACNGT 9 cut(s) 66, 271, 1135, 1415, 1600, 1692, 2645, 2707, 3575
HpyCH4IV ACGT 6 cut(s) 159, 823, 1215, 2670, 2677, 3528
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 176, 281, 284, 407, 627, 630, 2374
HpySE526I ACGT 6 cut(s) 159, 823, 1215, 2670, 2677, 3528
Hsp92I GRCGYC 1 cut(s) 3528
HspAI GCGC 2 cut(s) 401, 1326
KpnI GGTACC 1 cut(s) 2706
LmnI GCTCC 8 cut(s) 418, 491, 736, 1723, 1912, 2023, 2784, 3562
LweI GCATC 9 cut(s) 112, 157, 412, 530, 642, 1006, 2497, 2638, 3455
MaeII ACGT 6 cut(s) 159, 823, 1215, 2670, 2677, 3528
MaeIII GTNAC 7 cut(s) 131, 160, 937, 1821, 2568, 2671, 2724
MfeI CAATTG 3 cut(s) 1961, 2140, 2153
MhlI GDGCHC 3 cut(s) 181, 447, 1365
MlsI TGGCCA 1 cut(s) 3436
MluNI TGGCCA 1 cut(s) 3436
MlyI GAGTC 2 cut(s) 1033, 2344
MmeI TCCRAC 3 cut(s) 1117, 1974, 2067
Mox20I TGGCCA 1 cut(s) 3436
Mph1103I ATGCAT 3 cut(s) 1021, 1494, 1815
MroXI GAANNNNTTC 6 cut(s) 117, 599, 1215, 2424, 2603, 3406
MscI TGGCCA 1 cut(s) 3436
MseI TTAA 8 cut(s) 540, 1032, 1380, 1977, 2357, 2853, 3207, 3384
MslI CAYNNNNRTG 4 cut(s) 477, 1239, 1623, 2564
Msp20I TGGCCA 1 cut(s) 3436
MspI CCGG 4 cut(s) 198, 459, 1007, 2343
MspR9I CCNGG 5 cut(s) 155, 198, 391, 459, 1221
MunI CAATTG 3 cut(s) 1961, 2140, 2153
Mva1269I GAATGC 2 cut(s) 1183, 1494
MvaI CCWGG 3 cut(s) 155, 391, 1221
MvnI CGCG 2 cut(s) 1068, 2700
MwoI GCNNNNNNNGC 8 cut(s) 41, 176, 281, 284, 407, 627, 630, 2374
NciI CCSGG 2 cut(s) 198, 459
NcoI CCATGG 2 cut(s) 2298, 2453
NdeI CATATG 2 cut(s) 617, 1815
NheI GCTAGC 1 cut(s) 3187
NlaIV GGNNCC 6 cut(s) 463, 481, 732, 1725, 2704, 3496
NmuCI GTSAC 6 cut(s) 131, 160, 937, 1821, 2671, 2724
NruI TCGCGA 1 cut(s) 1068
NsiI ATGCAT 3 cut(s) 1021, 1494, 1815
NspI RCATGY 3 cut(s) 1238, 1315, 2086
OliI CACNNNNGTG 1 cut(s) 2564
PagI TCATGA 1 cut(s) 1926
PaqCI CACCTGC 1 cut(s) 526
PasI CCCWGGG 1 cut(s) 390
PceI AGGCCT 1 cut(s) 777
PciI ACATGT 2 cut(s) 1234, 2082
PcsI WCGNNNNNNNCGW 2 cut(s) 2934, 3111
PctI GAATGC 2 cut(s) 1183, 1494
PdmI GAANNNNTTC 6 cut(s) 117, 599, 1215, 2424, 2603, 3406
PfeI GAWTC 9 cut(s) 548, 595, 742, 748, 913, 1058, 1391, 1631, 1946
PflFI GACNNNGTC 1 cut(s) 1677
PflMI CCANNNNNTGG 1 cut(s) 2304
PfoI TCCNGGA 2 cut(s) 196, 1219
Ple19I CGATCG 1 cut(s) 2130
PleI GAGTC 2 cut(s) 1032, 2344
PmaCI CACGTG 1 cut(s) 2678
PmlI CACGTG 1 cut(s) 2678
PpsI GAGTC 2 cut(s) 1032, 2344
Ppu21I YACGTR 2 cut(s) 824, 2678
PscI ACATGT 2 cut(s) 1234, 2082
PshBI ATTAAT 3 cut(s) 540, 3207, 3384
Psp124BI GAGCTC 1 cut(s) 181
Psp1406I AACGTT 1 cut(s) 1215
Psp6I CCWGG 3 cut(s) 153, 389, 1219
PspCI CACGTG 1 cut(s) 2678
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 3 cut(s) 153, 389, 1219
PspN4I GGNNCC 6 cut(s) 463, 481, 732, 1725, 2704, 3496
PspPI GGNCC 1 cut(s) 2289
PstNI CAGNNNCTG 2 cut(s) 121, 1598
PsyI GACNNNGTC 1 cut(s) 1677
PvuI CGATCG 1 cut(s) 2130
RruI TCGCGA 1 cut(s) 1068
RsaI GTAC 5 cut(s) 24, 2020, 2081, 2403, 2704
RsaNI GTAC 5 cut(s) 23, 2019, 2080, 2402, 2703
RseI CAYNNNNRTG 4 cut(s) 477, 1239, 1623, 2564
SacI GAGCTC 1 cut(s) 181
SaqAI TTAA 8 cut(s) 540, 1032, 1380, 1977, 2357, 2853, 3207, 3384
Sau96I GGNCC 1 cut(s) 2289
SchI GAGTC 2 cut(s) 1033, 2344
ScrFI CCNGG 5 cut(s) 155, 198, 391, 459, 1221
SduI GDGCHC 3 cut(s) 181, 447, 1365
SfaNI GCATC 9 cut(s) 112, 157, 412, 530, 642, 1006, 2497, 2638, 3455
SfcI CTRYAG 3 cut(s) 182, 270, 396
SmiMI CAYNNNNRTG 4 cut(s) 477, 1239, 1623, 2564
SmlI CTYRAG 7 cut(s) 587, 837, 1370, 1760, 2488, 2636, 3026
SmoI CTYRAG 7 cut(s) 587, 837, 1370, 1760, 2488, 2636, 3026
SpeI ACTAGT 1 cut(s) 1825
SseBI AGGCCT 1 cut(s) 777
SsiI CCGC 6 cut(s) 621, 630, 2219, 2375, 2471, 2700
SspI AATATT 2 cut(s) 538, 1719
SstI GAGCTC 1 cut(s) 181
StuI AGGCCT 1 cut(s) 777
StyD4I CCNGG 5 cut(s) 153, 196, 389, 457, 1219
StyI CCWWGG 4 cut(s) 2298, 2453, 2787, 3509
TaaI ACNGT 9 cut(s) 66, 271, 1135, 1415, 1600, 1692, 2645, 2707, 3575
TaiI ACGT 6 cut(s) 162, 826, 1218, 2673, 2680, 3531
TaqII GACCGA 1 cut(s) 1355
TatI WGTACW 2 cut(s) 2079, 2401
TauI GCSGC 2 cut(s) 624, 633
TfiI GAWTC 9 cut(s) 548, 595, 742, 748, 913, 1058, 1391, 1631, 1946
Tru1I TTAA 8 cut(s) 540, 1032, 1380, 1977, 2357, 2853, 3207, 3384
Tru9I TTAA 8 cut(s) 540, 1032, 1380, 1977, 2357, 2853, 3207, 3384
TscAI CASTG 4 cut(s) 51, 1665, 2733, 2743
TseFI GTSAC 6 cut(s) 131, 160, 937, 1821, 2671, 2724
Tsp45I GTSAC 6 cut(s) 131, 160, 937, 1821, 2671, 2724
TspGWI ACGGA 2 cut(s) 2036, 3487
TspRI CASTG 4 cut(s) 51, 1665, 2733, 2743
Tth111I GACNNNGTC 1 cut(s) 1677
Van91I CCANNNNNTGG 1 cut(s) 2304
VspI ATTAAT 3 cut(s) 540, 3207, 3384
XapI RAATTY 6 cut(s) 310, 428, 985, 1047, 1981, 2581
XbaI TCTAGA 1 cut(s) 1870
XceI RCATGY 3 cut(s) 1238, 1315, 2086
XcmI CCANNNNNNNNNTGG 2 cut(s) 36, 897
XmiI GTMKAC 2 cut(s) 225, 701
XmnI GAANNNNTTC 6 cut(s) 117, 599, 1215, 2424, 2603, 3406
ZraI GACGTC 1 cut(s) 3529
Zsp2I ATGCAT 3 cut(s) 1021, 1494, 1815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.