FvH4_6g02760

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000203
Physical Location & Seq
Reverse (-)
1563728 .. 1573554
9827 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g02760.t1

Sequence Viewer

Length: 5643 bp
ATGGCAGATATTGTCATTTCAGTTGTTGGAAAGTTAGCAGAGTACACAATCGCACCTCTTCTGCGACAGTGTGGTTATCTGATTTACTACGAGAGGAATGTTGAAAATCTCACCGAGAAGGTTACCACTCTGACTGCTACAAGAGACGGGTTACAGTTACGTGTGGATGGAGCAGAGAGGAACTTGGAGGTTATTCTTCCCGAAGTTGAGAACTGGTTGGAACGAGTACAGCAGACCATACAAGAAAAGGAGACATGTTTTGAAAAAGGAAGAGTAGCCAAGGCAACAACATGCTGCAGCTCCAATGGGTGGTGTCCAAATCTGAAGGGTCGTCATTCCTTGAGTAGGAAAGCAGCGAAGATGGCTGAAAAGCTTGATAAGCTTATTACAGATGGAAATTTCCCCACAATATCATGTCCTGCTCCTCCGCCGAAGCTTGATTACCCAGCTATGCAATTTTATGAGGATTCCACTCAGGCCCTGGTGAAGCCACATGAAGGGGCTAGCTCCTCCTCTGGAACATCCAAACCTGCTGAAGTACATGTGCCTATGGATCTCTCTGAGCGTCTGGAATACAGGCTGCCTTATACAAAGGATCTCTTGACGGCTCTGCAAGATGACAAGATCAGCATGATTGGCATTTCTGGGACATTCAGAGAGATAGATACGGTAACAACCAAAGAGTTCACCAAAAGAATGAAACACAGAAATCTCTTTGACGAGGTTGTGATGGCAGTTGTGTCTCAGCATCCAGACTTGAAACGAATACAAGATGAGATTGCAGAAATGCTGGACCTGAAATTAGAGGAGAACGGTAGTTTGGTGGAACGGGCAGAGAGGCTACAAGCTCTTTTACTGGCGTCAGGTTCTAAGAGGATTCTTGTAATACTGACTGATGTCTGTGGAATACTTGATTTGGATGCAATTGGAATTCCTCATCTTGATATCATGGAAAGCTGCAAGATCGTGTTGATTTCAGAACTACAAGATGTGTTTGGTGAAATGGAGACTCAGAGCAATTTCAGATTGATATTACATTCCACTCCCAGAGCAAGACCTGTTAAGTTTGAGTCAAGAATTTCAGTCATCAGAGATGTAATGGATGCACTGACAGATGATGAATCCAATCCCATTGTAATATGGGGGATGGGGGGTATCGGGAAAACAACTTTGGTGAAGGAGATTGGTGCAAAAGCAGGTGAAGTACAACTATTTGATGAAATTGCAGTTGCAGAGTTCACCCAAGAACCTGATCTGTTCAAAATTCAAGGTCAAATTGCTAAAGCTCTAGGCCTGCAACTTACCGGAGAGGACAACAGAGCAGCCAAGTTACGTGAAAGATTATCACAGGGTAACAAAAATGTCCTTTTAATATTGGACAATGTCTGGACACAGCTAGATTTGTGGGAAATAGGGATTCCAATTTCCCGTGATCAAAAATGTTGTAAACTTCTGGTAACATCACGGGACCGAGATATTTTCAAAGAGATGAAAACCAGAAAACATTTCCACATTGGTGGTTTGCCAAAACATGATGCCTGGAGTTTGTTTAAGAAGGTGGCAGGAGGTTCCATTGAATCCGATCTCAAGCTGCGTCCAATAGCAGAACAAGTTCTTGCTGAATGTGATGGTTTACCAGTTGCAATTTCTACAGTTGGAAGGGCACTACAAGGTAAAAGTATTTCAAGCTGGAGAGATGCATGGAGAGAACAAAGGTATGCTTGTCCCGAAAACGTGCCTGGAGTGATAAAGCATGTGTATAGACCAATAATGCTCAGCTACGAATGTCTACCAAGCCAGCAAGCCAAGTCGTGTTTTTTGCTATGTTGTCTATTTCGAGAAAGTGAAGATTTTCATATTGAAATGTTGGTTAGAATTGCGATTGGGCTTGGGCTTTTGAAACGCATTGATTCAATATCTCAAGGAAGGGATTGCGTAGAATCATTGGTTGACACACTCAAAAGTCGTTCCTTATTGTTAGACGGGGATTGGAAACAGTGTGTCAGAATGCATGATGTGGTGCGTGATGTAGCCGTGTATATAGCCTCCCAAGAGTTTGCACAAAAGAAGAATGATGAGTCTGAAAAGAGAATCGTGTTAAGACATGGACCGGAAGTGAACAAGAATAATTTTGTCGATGAATCTGGATCTGAGGAATCTGCATATTGTCGTTCACTGACAGCCTGTACAAGTTCTACTGAACTTTTACTAATAATAGGGGTCAAAGATCGGTTGAAGCTGCGGGCCACAATTTTCGATGGAATGGAAGACCTTAAGGTTTTATTGATCTGGCATTCAATCCTCCCATCCCTTCCACTTTTGAAAAATATTCAAACACTTGTTCTAGCGGAGTGCCATCTCAATTTTAATGTTGATGTGATTGGAGAGCTACGGGCACTCATGATACTCGATTTAAGTGAGTCTAACATCAAGCAGTTGCCTGATACATTCAAAAATTTATCCAATCTCAGAGTGTTAAATTTATCGGAATGTGGGGAGCTGAAAGTAATCTCACCCGGTGTTATATCGAGTTTATCTCGACTAGAAGAATTGCGCATGTGGCGTAGCTTTCAAGATTGGGTGGTTGAGAAACTGGGCTCAGCAGAAACAACCAATTGGATCTCTCAATTGGAGGACGAGGATGCCGAACCAGAACAAGTCGACAGGTGGAAGAGACTCAACAGATTGGCGGTTGAGAAACCAGCCTCAACAGAAACAACAACTGATCGGGTGTCTCAACTGGAGGATGACGAAAGCAGCAAACAAGAGGAAGTCAACAAGCTGTTCCGAGACTTTGTTGATGATGTTTATAAGGCAGAAGGAAACTACGACCAGGTCACAAGGTGGGCCAACTCTATGGACTGGAGAGTCGGGTTACTTTCAGAGCTGCTCTCCTTGTCCCGTTTGACTACTTTGGAAGTGTTTTTACCACCTATTGAGATTGTGCGGACCAGTAAGCTGTTTCATAATCTTGACAGATTTGACATCTCTATAGGGTGGGGGAGTCATGCTTATCGGGTAGAGGGTAATTACTTGAGGGTTAAGGATCTAGATCTTACGGGTACTGGAATCAGCTTGTTGTTAAAGAAAACCAAGAGACTCCGGTTAAAGATGAAGAGTTTGACAGATCCTCTCAATGTGTGGGATGTAAACTGTTGTGCAACCTTGAAGTCACTTACCCTTGAACACTGTGATTCTCTAGAATATCTGATTGACACGACACTTGAGAAACATACTCCACAAAGTGTCTTCCCTGTCTTGGGTTATTTGAGAATCGAGAGAGCAAAGAGGCTGAAAGAGATTTTTCATGGTGACCTGCCACCAGGCTCGCTTCAGAAACTAGAAACGCTATGGTTGTATGGTCTTCCTGAATTGACGAACATTTGGACTATTGAAAGTCAATCTAGATGGATGGGAGATCAACTGGCAGTGCTCGAGTCCATAACAATAAGCGATTGCCCGTCCGTAGAAGCAATTTTTGGCTTGGAAGGATCGGAACATCATGTCAAAGAGATCAGCTTCCTTAGTTTGAAATCCTTGCGACTCCAAGGTCTACCATGCTTCACCGGGATCAGCAAAAGTAGGCTGAATAGGCATCAGGAAGTCAGCAGTCTCTACCGGGATAGTTTGACTGATGAGCATTCACTTTTCTCCGACCCCAAGGTTATCTTTCCAGTATTGAGAATCCTATCCATTGAACGCCTGGGCGAGTTGAAAAAGATATGGAATGGCCAACTTTCACCCACCTCCTTCTCTAAATTAGAAAGACTCACTGTCTCGGGATGTGACAAACTACTGCATTTGGTACCGACACAAATGCAAAATAGATTGCAGACATTGGAGTCCATACGAGTAGAGGAATGTTCTTCACTGGAAGGGATTTTTGAAGTGAGCAGATTGACCGTCAATGAAAGGAATGCTTCAGTTTCTCGGTCAGACAAATTACCTTCAAATATTTCCCAACCTGATCAAGGAATGCAAATAAACGATATCATGGGTTGCAGACAATCTTGCCAAGGCTTTCAGAATTTAACTGGACTGGATATTACATCTTGTGGTAGGTTGAGATACCTGCTCTCTCCTTCGATTGCTAGAGGTCTTGTCAAGCTCCAAATACTAGAGATAAAGGATTGCCAGAAGATGGAAGCAATAGTCACAGCAGATGAAGGTGAAGAAACTGAGAATGAAAGCATGCTCCCTCGGCTAAATTTTTTGGATCTTCGGAATCTACCAAATCTTGGAAGCTTTTCGCAAGGCAGATATACTTTTGATTGGCCACTAGTGAAATGGATCCGTATTATCAAGTGCAACAAAATGAACAAATTTTGTTCAGGATCCCTCCGCATATCCAGGGCAGTGGACATAACTGTCCGATCTTCTGGTAAGAGTAGTAGCTTGGTACAGGTCAATTTCTCAAGTGATTATATACTGAGGGAGCAGTATAAGGATCCTTGGAATGAGGTCAGAATTGGGAAGCTGCTTGAAGACCTTGATGCTTTAGCTGGTACCATCTCTGTCAAGCACTGCTATGCTGTTGATACCACAACAAGGCCACTCTTACTGGTCACTGCATCTGTTGACAAAATTGTCCTGAAGAAGCCAATTAGTGTTGACATTGACCTGGAAATTGTTGGTGCTGTCATTTGGGTGGGGCGGTCATCTATTGAGATCCAACTAGAAGTTACTCAATCCACAAAAGACATTTATCAATTGATATGTGATGAGAAACAGACTGATGTACCCAGGCCGGACCATAATTTGCAAGATATCAGGGCCAAGATATTTCCTCTTAAAAGACGTAAAATTAATGCACCCGAATCACCTTCAGTTTCATTACCGGTGAAAAGGAAGGAGAGGTCATTATCATCATTAGTAGTCAGCACCCGCAAAGTTCCAGTGCAGGCAGGCTTGACTGGAAAGAGACCAAGAGCCTATCTGAGTTCTCTAGCAACTTATTTAAGTTCTAGGAAGTCAGGTCTTTCTCCTAAGGAAACCCTGGATTCTTCCATGCCTGAGAATTCCCATGAGCAGAAGCCTAAGAAAGATGATGTTGAAGTAATGGAAGGCAAAGCTGATCTGTGGACACCCTTAAACTGTCTTGTAGAGGCTGCTAACCGAACCAAGTCCTCTAAGTTAAATCCACAAGGGCAGTTGCCTCTCAATGCCCTTGATAGTATTTCTGAACTTTATATGCCTGAAAATAACAGAGACATAGAATCGCCAGATGCTCTAGAAAGTCATGTACATGTGTCTAAGGTGAAAATCAAGGAGCCCGAGAACAGTACTAGAGGAAAAAAGAACATTAATGCGGCAGCAGTCACAGGATCCATGAAACGGAAGAGGTTTCATGCAGGTAATCGTAATAGGGGAGCTCCATCTGGGGAGTCAGGTGATTCAACTCAAGATGTACGTGGGGCTAAGCATAGCAGAAGAAATAATCCTATTTGGTTCTTGTTGGTTGCAGCTGAAAACTGGAAAAGAGAGGCCTCCTTGCCCCAGATATCATCATGTTACCTGAGAATAAAGGATGTTAAAATGCCTGTAACATTTATTCAGAAGTACCTTGTGAAGAAACTTGAACTTACCAGTGAAGCTGAGACTCTCAGTGCCTTCCTCTTACTCTGCAACGCTTTTGTTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1881

Amino Acids

212.01

Weight (kDa)

6.61

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 222 - 339 1e-08 NB-ARC domain
NB-ARC PF00931 363 - 521 3.8e-26 NB-ARC domain
LRR_14 PF23598 793 - 867 3.8e-08 Leucine-rich repeat region
LRR_8 PF13855 799 - 852 1.8e-08 Leucine rich repeat
LRR_RPS2 PF23247 1237 - 1379 2.5e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1338 - 1440 1.4e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2811
AarI CACCTGC 1 cut(s) 1187
AasI GACNNNNNNGTC 5 cut(s) 2867, 3549, 3841, 4367, 4586
Acc16I TGCGCA 1 cut(s) 2555
Acc36I ACCTGC 5 cut(s) 538, 1187, 3324, 4080, 5342
Acc65I GGTACC 2 cut(s) 3805, 4505
AccB1I GGYRCC 2 cut(s) 3805, 4505
AccB7I CCANNNNNTGG 3 cut(s) 309, 4141, 4238
AccI GTMKAC 3 cut(s) 1789, 2661, 3553
AciI CCGC 9 cut(s) 428, 2242, 2348, 2690, 2947, 4342, 4654, 4885, 5309
AcoI YGGCCR 2 cut(s) 3730, 4274
AcuI CTGAAG 6 cut(s) 344, 555, 3317, 3906, 4613, 4809
AcyI GRCGYC 1 cut(s) 860
AdeI CACNNNGTG 3 cut(s) 2519, 2844, 3245
AflII CTTAAG 1 cut(s) 2273
AflIII ACRYGT 4 cut(s) 160, 254, 541, 5245
AgeI ACCGGT 1 cut(s) 4837
AhdI GACNNNNNGTC 1 cut(s) 3773
AhlI ACTAGT 1 cut(s) 4279
AjuI GAANNNNNNNTTGG 6 cut(s) 803, 835, 1154, 1186, 3462, 3494
AleI CACNNNNGTG 1 cut(s) 1515
Alw21I GWGCWC 2 cut(s) 3435, 5374
AlwNI CAGNNNCTG 1 cut(s) 481
Ama87I CYCGRG 3 cut(s) 3434, 3778, 5273
AseI ATTAAT 2 cut(s) 4806, 5304
AsiGI ACCGGT 1 cut(s) 4837
Asp700I GAANNNNTTC 3 cut(s) 1611, 1851, 4246
Asp718I GGTACC 2 cut(s) 3805, 4505
AspLEI GCGC 1 cut(s) 2556
AspS9I GGNCC 9 cut(s) 478, 793, 1468, 2108, 2244, 2847, 2949, 4750, 4773
AsuC2I CCSGG 3 cut(s) 2517, 3568, 3620
AsuNHI GCTAGC 1 cut(s) 503
AvaI CYCGRG 3 cut(s) 3434, 3778, 5273
AvaII GGWCC 5 cut(s) 793, 1468, 2108, 2949, 4750
AxyI CCTNAGG 1 cut(s) 4986
BaeGI GKGCMC 2 cut(s) 1666, 2398
BaeI ACNNNNGTAYC 2 cut(s) 4722, 4755
BalI TGGCCA 2 cut(s) 3732, 4276
BamHI GGATCC 4 cut(s) 4290, 4334, 4447, 5324
BanI GGYRCC 2 cut(s) 3805, 4505
BanII GRGCYC 3 cut(s) 2600, 5274, 5374
BarI GAAGNNNNNNTAC 4 cut(s) 425, 457, 2910, 2942
BbsI GAAGAC 4 cut(s) 2274, 3241, 3356, 4491
Bbv12I GWGCWC 2 cut(s) 3435, 5374
BceAI ACGGC 2 cut(s) 621, 2018
BcgI CGANNNNNNTGC 2 cut(s) 3799, 3833
BclI TGATCA 2 cut(s) 1432, 3967
BcnI CCSGG 3 cut(s) 2517, 3568, 3620
BcuI ACTAGT 1 cut(s) 4279
BfmI CTRYAG 3 cut(s) 295, 1650, 2991
BfrI CTTAAG 1 cut(s) 2273
BfuAI ACCTGC 5 cut(s) 538, 1187, 3324, 4080, 5342
BglII AGATCT 1 cut(s) 3052
BlpI GCTNAGC 3 cut(s) 1775, 2599, 5418
BmcAI AGTACT 1 cut(s) 5284
Bme18I GGWCC 5 cut(s) 793, 1468, 2108, 2949, 4750
BmeRI GACNNNNNGTC 1 cut(s) 3773
BmeT110I CYCGRG 3 cut(s) 3434, 3778, 5273
BmgT120I GGNCC 9 cut(s) 478, 793, 1468, 2108, 2244, 2847, 2949, 4750, 4773
BmiI GGNNCC 9 cut(s) 1469, 1570, 3807, 4292, 4336, 4449, 4507, 5271, 5326
BmrI ACTGGG 1 cut(s) 2603
BmtI GCTAGC 1 cut(s) 507
BmuI ACTGGG 1 cut(s) 2603
BoxI GACNNNNGTC 1 cut(s) 896
BpiI GAAGAC 4 cut(s) 2274, 3241, 3356, 4491
BplI GAGNNNNNCTC 4 cut(s) 2521, 2553, 2876, 2908
BpmI CTGGAG 5 cut(s) 1561, 1711, 1761, 2762, 2884
Bpu1102I GCTNAGC 3 cut(s) 1775, 2599, 5418
BpuEI CTTGAG 7 cut(s) 361, 1571, 1905, 3055, 3245, 4399, 5385
BpuMI CCSGG 3 cut(s) 2517, 3568, 3620
BsaAI YACGTR 3 cut(s) 161, 1334, 5411
BsaBI GATNNNNATC 1 cut(s) 3051
BsaHI GRCGYC 1 cut(s) 860
BsaI GGTCTC 1 cut(s) 4915
BsaWI WCCGGW 4 cut(s) 1304, 2110, 3102, 4837
Bse118I RCCGGY 1 cut(s) 4837
Bse21I CCTNAGG 1 cut(s) 4986
Bse8I GATNNNNATC 1 cut(s) 3051
BseJI GATNNNNATC 1 cut(s) 3051
BseRI GAGGAG 4 cut(s) 414, 499, 502, 821
BseSI GKGCMC 2 cut(s) 1666, 2398
BseYI CCCAGC 1 cut(s) 445
BsgI GTGCAG 1 cut(s) 4919
BshNI GGYRCC 2 cut(s) 3805, 4505
BshTI ACCGGT 1 cut(s) 4837
BsiHKAI GWGCWC 2 cut(s) 3435, 5374
BsiHKCI CYCGRG 3 cut(s) 3434, 3778, 5273
BsiSI CCGG 8 cut(s) 1305, 2111, 2517, 3103, 3567, 3619, 4748, 4838
BslFI GGGAC 4 cut(s) 661, 1481, 1710, 2884
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 4 cut(s) 661, 1481, 1710, 2884
BsmI GAATGC 5 cut(s) 2013, 2293, 3640, 3922, 3981
Bso31I GGTCTC 1 cut(s) 4915
BsoBI CYCGRG 3 cut(s) 3434, 3778, 5273
Bsp1286I GDGCHC 6 cut(s) 1666, 2398, 2600, 3435, 5274, 5374
Bsp1407I TGTACA 2 cut(s) 2186, 5242
Bsp1720I GCTNAGC 3 cut(s) 1775, 2599, 5418
BspACI CCGC 9 cut(s) 428, 2242, 2348, 2690, 2947, 4342, 4654, 4885, 5309
BspHI TCATGA 1 cut(s) 2400
BspLI GGNNCC 9 cut(s) 1469, 1570, 3807, 4292, 4336, 4449, 4507, 5271, 5326
BspMAI CTGCAG 1 cut(s) 299
BspMI ACCTGC 5 cut(s) 538, 1187, 3324, 4080, 5342
BspOI GCTAGC 1 cut(s) 507
BspT107I GGYRCC 2 cut(s) 3805, 4505
BspTI CTTAAG 1 cut(s) 2273
BspTNI GGTCTC 1 cut(s) 4915
BsrFI RCCGGY 1 cut(s) 4837
BsrGI TGTACA 2 cut(s) 2186, 5242
BssAI RCCGGY 1 cut(s) 4837
BssNI GRCGYC 1 cut(s) 860
BssT1I CCWWGG 5 cut(s) 279, 3547, 3660, 4015, 4451
Bst6I CTCTTC 5 cut(s) 63, 265, 2666, 3110, 5333
BstACI GRCGYC 1 cut(s) 860
BstAFI CTTAAG 1 cut(s) 2273
BstAUI TGTACA 2 cut(s) 2186, 5242
BstBAI YACGTR 3 cut(s) 161, 1334, 5411
BstC8I GCNNGC 9 cut(s) 505, 1295, 1799, 1803, 2244, 3329, 4193, 4902, 4906
BstEII GGTNACC 2 cut(s) 121, 3311
BstENI CCTNNNNNAGG 2 cut(s) 343, 3969
BstHHI GCGC 1 cut(s) 2556
BstMWI GCNNNNNNNGC 6 cut(s) 362, 379, 636, 2560, 3592, 4201
BstNSI RCATGY 7 cut(s) 258, 294, 545, 1757, 2560, 4195, 5249
BstPAI GACNNNNGTC 1 cut(s) 896
BstPI GGTNACC 2 cut(s) 121, 3311
BstSFI CTRYAG 3 cut(s) 295, 1650, 2991
BstSLI GKGCMC 2 cut(s) 1666, 2398
BstV2I GAAGAC 4 cut(s) 2274, 3241, 3356, 4491
BstXI CCANNNNNNTGG 3 cut(s) 1517, 2857, 4357
Bsu36I CCTNAGG 1 cut(s) 4986
BtsI GCAGTG 4 cut(s) 3435, 4362, 4522, 4566
BveI ACCTGC 5 cut(s) 538, 1187, 3324, 4080, 5342
Cac8I GCNNGC 9 cut(s) 505, 1295, 1799, 1803, 2244, 3329, 4193, 4902, 4906
CaiI CAGNNNCTG 1 cut(s) 481
CciI TCATGA 1 cut(s) 2400
CfoI GCGC 1 cut(s) 2556
Cfr10I RCCGGY 1 cut(s) 4837
Cfr13I GGNCC 9 cut(s) 478, 793, 1468, 2108, 2244, 2847, 2949, 4750, 4773
CseI GACGC 3 cut(s) 554, 849, 1583
CsiI ACCWGGT 1 cut(s) 2832
CspAI ACCGGT 1 cut(s) 4837
DraIII CACNNNGTG 3 cut(s) 2519, 2844, 3245
DrdI GACNNNNNNGTC 5 cut(s) 2867, 3549, 3841, 4367, 4586
DriI GACNNNNNGTC 1 cut(s) 3773
DseDI GACNNNNNNGTC 5 cut(s) 2867, 3549, 3841, 4367, 4586
EaeI YGGCCR 2 cut(s) 3730, 4274
Eam1104I CTCTTC 5 cut(s) 63, 265, 2666, 3110, 5333
Eam1105I GACNNNNNGTC 1 cut(s) 3773
EarI CTCTTC 5 cut(s) 63, 265, 2666, 3110, 5333
EciI GGCGGA 1 cut(s) 417
Ecl136II GAGCTC 1 cut(s) 5372
Eco130I CCWWGG 5 cut(s) 279, 3547, 3660, 4015, 4451
Eco147I AGGCCT 2 cut(s) 1293, 5486
Eco24I GRGCYC 3 cut(s) 2600, 5274, 5374
Eco31I GGTCTC 1 cut(s) 4915
Eco32I GATATC 4 cut(s) 946, 3991, 4768, 5502
Eco47I GGWCC 5 cut(s) 793, 1468, 2108, 2949, 4750
Eco53kI GAGCTC 1 cut(s) 5372
Eco57I CTGAAG 6 cut(s) 344, 555, 3317, 3906, 4613, 4809
Eco81I CCTNAGG 1 cut(s) 4986
Eco88I CYCGRG 3 cut(s) 3434, 3778, 5273
Eco91I GGTNACC 2 cut(s) 121, 3311
EcoICRI GAGCTC 1 cut(s) 5372
EcoNI CCTNNNNNAGG 2 cut(s) 343, 3969
EcoO109I RGGNCCY 1 cut(s) 478
EcoO65I GGTNACC 2 cut(s) 121, 3311
EcoRI GAATTC 2 cut(s) 930, 5017
EcoRV GATATC 4 cut(s) 946, 3991, 4768, 5502
EcoT14I CCWWGG 5 cut(s) 279, 3547, 3660, 4015, 4451
EcoT22I ATGCAT 2 cut(s) 1702, 2013
EcoT38I GRGCYC 3 cut(s) 2600, 5274, 5374
ErhI CCWWGG 5 cut(s) 279, 3547, 3660, 4015, 4451
Esp3I CGTCTC 1 cut(s) 138
FalI AAGNNNNNCTT 8 cut(s) 584, 616, 1705, 1737, 3653, 3685, 3904, 3936
FaqI GGGAC 4 cut(s) 661, 1481, 1710, 2884
FauI CCCGC 2 cut(s) 2235, 4892
FbaI TGATCA 2 cut(s) 1432, 3967
FblI GTMKAC 3 cut(s) 1789, 2661, 3553
FriOI GRGCYC 3 cut(s) 2600, 5274, 5374
FspI TGCGCA 1 cut(s) 2555
GlaI GCGC 1 cut(s) 2555
GsaI CCCAGC 1 cut(s) 449
GsuI CTGGAG 5 cut(s) 1561, 1711, 1761, 2762, 2884
HapII CCGG 8 cut(s) 1305, 2111, 2517, 3103, 3567, 3619, 4748, 4838
HgaI GACGC 3 cut(s) 554, 849, 1583
HhaI GCGC 1 cut(s) 2556
Hin1I GRCGYC 1 cut(s) 860
Hin6I GCGC 1 cut(s) 2554
HinP1I GCGC 1 cut(s) 2554
HincII GTYRAC 5 cut(s) 1951, 2662, 2776, 4579, 4612
HindII GTYRAC 5 cut(s) 1951, 2662, 2776, 4579, 4612
HindIII AAGCTT 4 cut(s) 371, 380, 434, 4243
HpaII CCGG 8 cut(s) 1305, 2111, 2517, 3103, 3567, 3619, 4748, 4838
HpyCH4IV ACGT 5 cut(s) 160, 1333, 1734, 4798, 5410
HpyF10VI GCNNNNNNNGC 6 cut(s) 362, 379, 636, 2560, 3592, 4201
HpySE526I ACGT 5 cut(s) 160, 1333, 1734, 4798, 5410
Hsp92I GRCGYC 1 cut(s) 860
HspAI GCGC 1 cut(s) 2554
KpnI GGTACC 2 cut(s) 3809, 4509
Ksp22I TGATCA 2 cut(s) 1432, 3967
MabI ACCWGGT 1 cut(s) 2832
MaeII ACGT 5 cut(s) 160, 1333, 1734, 4798, 5410
MfeI CAATTG 4 cut(s) 924, 2614, 2627, 4709
MhlI GDGCHC 6 cut(s) 1666, 2398, 2600, 3435, 5274, 5374
MlsI TGGCCA 2 cut(s) 3732, 4276
MluNI TGGCCA 2 cut(s) 3732, 4276
MmeI TCCRAC 5 cut(s) 7, 198, 1636, 3678, 4696
Mox20I TGGCCA 2 cut(s) 3732, 4276
Mph1103I ATGCAT 2 cut(s) 1702, 2013
MroXI GAANNNNTTC 3 cut(s) 1611, 1851, 4246
MscI TGGCCA 2 cut(s) 3732, 4276
MslI CAYNNNNRTG 4 cut(s) 1515, 4527, 4646, 5244
Msp20I TGGCCA 2 cut(s) 3732, 4276
MspA1I CMGCKG 1 cut(s) 5465
MspCI CTTAAG 1 cut(s) 2273
MspI CCGG 8 cut(s) 1305, 2111, 2517, 3103, 3567, 3619, 4748, 4838
MunI CAATTG 4 cut(s) 924, 2614, 2627, 4709
Mva1269I GAATGC 5 cut(s) 2013, 2293, 3640, 3922, 3981
MwoI GCNNNNNNNGC 6 cut(s) 362, 379, 636, 2560, 3592, 4201
NciI CCSGG 3 cut(s) 2517, 3568, 3620
NheI GCTAGC 1 cut(s) 503
NlaIV GGNNCC 9 cut(s) 1469, 1570, 3807, 4292, 4336, 4449, 4507, 5271, 5326
NmeAIII GCCGAG 1 cut(s) 4180
NmuCI GTSAC 7 cut(s) 2836, 3171, 3311, 3785, 4153, 4564, 5317
NsbI TGCGCA 1 cut(s) 2555
NsiI ATGCAT 2 cut(s) 1702, 2013
NspI RCATGY 7 cut(s) 258, 294, 545, 1757, 2560, 4195, 5249
OliI CACNNNNGTG 1 cut(s) 1515
PaeI GCATGC 1 cut(s) 4195
PaeR7I CTCGAG 1 cut(s) 3434
PagI TCATGA 1 cut(s) 2400
PaqCI CACCTGC 1 cut(s) 1187
PceI AGGCCT 2 cut(s) 1293, 5486
PciI ACATGT 3 cut(s) 254, 541, 5245
PcsI WCGNNNNNNNCGW 1 cut(s) 2643
PctI GAATGC 5 cut(s) 2013, 2293, 3640, 3922, 3981
PdmI GAANNNNTTC 3 cut(s) 1611, 1851, 4246
PflFI GACNNNGTC 2 cut(s) 1382, 2834
PflMI CCANNNNNTGG 3 cut(s) 309, 4141, 4238
PinAI ACCGGT 1 cut(s) 4837
Ppu21I YACGTR 3 cut(s) 161, 1334, 5411
PscI ACATGT 3 cut(s) 254, 541, 5245
PshAI GACNNNNGTC 1 cut(s) 896
PshBI ATTAAT 2 cut(s) 4806, 5304
PsiI TTATAA 1 cut(s) 2811
Psp124BI GAGCTC 1 cut(s) 5374
PspEI GGTNACC 2 cut(s) 121, 3311
PspFI CCCAGC 1 cut(s) 445
PspN4I GGNNCC 9 cut(s) 1469, 1570, 3807, 4292, 4336, 4449, 4507, 5271, 5326
PspPI GGNCC 9 cut(s) 478, 793, 1468, 2108, 2244, 2847, 2949, 4750, 4773
PspXI VCTCGAGB 1 cut(s) 3434
PstI CTGCAG 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 481
PsyI GACNNNGTC 2 cut(s) 1382, 2834
PvuII CAGCTG 1 cut(s) 5465
RseI CAYNNNNRTG 4 cut(s) 1515, 4527, 4646, 5244
SacI GAGCTC 1 cut(s) 5374
SalI GTCGAC 1 cut(s) 2660
Sau96I GGNCC 9 cut(s) 478, 793, 1468, 2108, 2244, 2847, 2949, 4750, 4773
ScaI AGTACT 1 cut(s) 5284
SduI GDGCHC 6 cut(s) 1666, 2398, 2600, 3435, 5274, 5374
SexAI ACCWGGT 1 cut(s) 2832
SfcI CTRYAG 3 cut(s) 295, 1650, 2991
Sfr274I CTCGAG 1 cut(s) 3434
SinI GGWCC 5 cut(s) 793, 1468, 2108, 2949, 4750
SlaI CTCGAG 1 cut(s) 3434
SmiMI CAYNNNNRTG 4 cut(s) 1515, 4527, 4646, 5244
SmlI CTYRAG 9 cut(s) 340, 1586, 1920, 2273, 3034, 3224, 3434, 4414, 5400
SmoI CTYRAG 9 cut(s) 340, 1586, 1920, 2273, 3034, 3224, 3434, 4414, 5400
SpeI ACTAGT 1 cut(s) 4279
SphI GCATGC 1 cut(s) 4195
SseBI AGGCCT 2 cut(s) 1293, 5486
SsiI CCGC 9 cut(s) 428, 2242, 2348, 2690, 2947, 4342, 4654, 4885, 5309
SspI AATATT 3 cut(s) 1374, 2329, 3955
SstI GAGCTC 1 cut(s) 5374
StuI AGGCCT 2 cut(s) 1293, 5486
StyI CCWWGG 5 cut(s) 279, 3547, 3660, 4015, 4451
TaiI ACGT 5 cut(s) 163, 1336, 1737, 4801, 5413
TaqII GACCGA 2 cut(s) 1485, 3921
TatI WGTACW 7 cut(s) 42, 226, 538, 1204, 2186, 5242, 5282
TauI GCSGC 1 cut(s) 5312
TseFI GTSAC 7 cut(s) 2836, 3171, 3311, 3785, 4153, 4564, 5317
Tsp45I GTSAC 7 cut(s) 2836, 3171, 3311, 3785, 4153, 4564, 5317
TspGWI ACGGA 3 cut(s) 3454, 4283, 5350
Tth111I GACNNNGTC 2 cut(s) 1382, 2834
Van91I CCANNNNNTGG 3 cut(s) 309, 4141, 4238
Vha464I CTTAAG 1 cut(s) 2273
VpaK11BI GGWCC 5 cut(s) 793, 1468, 2108, 2949, 4750
VspI ATTAAT 2 cut(s) 4806, 5304
XagI CCTNNNNNAGG 2 cut(s) 343, 3969
XbaI TCTAGA 4 cut(s) 3049, 3199, 3404, 5230
XceI RCATGY 7 cut(s) 258, 294, 545, 1757, 2560, 4195, 5249
XcmI CCANNNNNNNNNTGG 3 cut(s) 478, 3700, 4284
XhoI CTCGAG 1 cut(s) 3434
XmiI GTMKAC 3 cut(s) 1789, 2661, 3553
XmnI GAANNNNTTC 3 cut(s) 1611, 1851, 4246
ZrmI AGTACT 1 cut(s) 5284
Zsp2I ATGCAT 2 cut(s) 1702, 2013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.