Rw1G014320

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000203
Physical Location & Seq
Forward (+)
31994683 .. 31999609
4927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014320.1

Sequence Viewer

Length: 3924 bp
ATGCAAGATATTATTCTAGCAGTTGCTACACGATTGGCAGAATGGACGGTGAGACCAATTTGGCGGCAGTTCAGTTATGTGGTTCAGTACAAGAGCAACATTGAGCATCTCACCAGACAGGTTCAGGAACTGCGTGATAAAAGATATGGAGTGGACATGGAGGTGAAACCTGCTACAGAAGCTTCAAAGATTATTGATCCTGGAGTTAAACATTGGCTGGCTGAGGTAGACAAGCTGATAGGAGAAAAGGAGAAGTGTTTCAATGAAGAGACTGTAGCAACAAAGGCAGCATGCTGTAATGGGTCTTTTCCAGATTTGAAGTGCCGTTATTCTCTAGGAAGGAAAGCTAAGAAGATGACTATGGTTTTTGATTACCTCCTCGCTAAAGATCTCGGGACTATAGCGCATCCTGCTCTTCCCCCAGAGGTGGAATTTCAACCCACACAATATTCTGAGGATTCTTCTCAGCTTGCAACCAACTTCCAAGAAGGTGCCAGCTCCTCAGAAACATCTAACCAAATTCAGCCGCCTACATCTACAGGAAAAAATATTGATTTTGAATCGAGGAGATCGACCATTAGAAGTGTAATGGAAGCTGTGAAGGGGAATGAGATCAATCCGGTTGTCATATGTGGCATGGGAGGTATTGGGAAGACAACATTGATGGGGGAAGTTGTTAACAGAGCAAAGAAAGAGGGTCTGTTTGATGAGTATACAAAGGCAACTGTTAAAGAAACTCTTGACAAAACTCCTGACATTTGTCTGATTCAAGATGAACTTGCTCAATATCTAGGTCTTCCACTTGAAGGAAAAGAAATAGATGCAAGAGCAAACAAGATACGTCAAAGATTATCTGCAGGTGCCAAGAAGGTTCTTGTAATATTAGACAATGTTTCGACAACAAACCCAGACTTTTTGTGGGAAGTAGGGATTCCTCGTGATCAGAATAGTTGTAAGCTTTTAGTAACATCAAGAGAACGAGATGTATTCAAGGACATTGACACAAAAAAGAATTTCCCCATCAGTGATTTGCCAGAAAATGAGGCCTGGAGGTTGTTCAAGAAGACAGCAGGAGGTTTGATTGAATCTGATCCCAATTTGCATTTGGTAGCAAAACAGGTTGTGAAGGAATGTGCAGGGTTGCCAATTGCAATTTCAACTGTTGGAAGGGCACTACATGGTGAAGGAATTGCAATTTGGAAGAATGCACTTAGGGAAATGGAAAAGGCTTGCCCAGAAAATGTTCCAGGAGTGATAGATCATGTGTATGGAAAAATAAAATTCAGTTATGAATGTTTACAAAGCAAGCAAGCCAAATCATGCTTTCTGTTATGTTGCATATTTGCAGAAAGCGTTGATATACCGATTGAATATTTTGTTAGATTTGGGCTTGGGCTTGGGATCTTTAAAGGCATTGATTCAATGGCTGAAGGAAGAAACTGTATAGAAACATTGGTTCACACATTAAAAAGTCGCTTTCTATTGTTGGACAGTGATAAGAAAGAGTGTGTGAGAATGCATGATGTGGTGCGCGATGTAGCCTTGCATATAGCCTCTGAAGGGTTGACTCAAAAGAAAAGTGATGAGGTTAAAGAGAGAATTGTGGTAAGACCCTCGGTGGAAACTGTGAAGTCTATGGATTGGCCAAAGAGTAGTGTTTCCAAGCAATCTACTTACTGCAGTTCACTGTGTATTGGTGGGCTTTCGGAGCTCTTACTGTTAAGATGCATAACTGAAAGTGGATTGGAGCCGCCACCCACAATTTTCAATAGAATGGAAGATCTCAAGGTTCTCGTAATTGCAGGTATTTCTCTATTGTCAATACTGCCATCACTTCCAGTTTTGAGAAACCTTCAAACATTGTGTTTGGAGGATTGCTATCTAAACTTTGATGTAGTATCTGTCATTGGGGAGATAAGGACACTCATGATACTCAGTTTACGTGGATCTGACATCAAACAACTGCCTGATACATTTAAAAATTTATCTAATCTAAGATTACTTGATTTGTCTAGATGTATGAAACTTGAGATAATCTCACCAGGTGTCATATCAAGTTTATCCCGACTAGAAGAGTTGTATATGTGGGATAGCTTTGAAGATTGGGTGGTTGAGAAACTAGCCTCAACAGAAACAACCAATTGGATTTCTCAATTGGAAGCTGAGACAAGAGCTAAACCAGAAGAACTCCATGTGGAACTGGAGATGTGGAAGAGATTCAACAGATTGGCGGTTGAGCAACCAGCATATCTAGGAAAGATGTACAGAGAGAAGGTTCAAAATTTATATACGAAAGACGAGGAGCACACATACAGAAGGTGGGCCAGCTCCATGGACTGGAAAATTGGGATACTTTCAGAGCTACTTTCCTTGTCCAGTTTGACTACTTTAGAAGTTGTTCTACCACCGGTTGATAGTCTGGTAACCAGCAAGCTATTTGACAAGCTTGAAAGATTTAAGATCTCTATAGGATGGAAGGAGCATATATATAATTGGGAATTAGAGGCTTATGGAAACTACTTGAGAGTTCATGATCTTGATGCAAGCTCTCTTGTGAGTACTGGAATCACTTTGTTGCTAAAGAAAACCAAGACTTTTGAGTTAAAAATGAAGAATTTGACAGAACCTCTGAATGTCTTAGATACAGAGTGCTGTTTTGCAAACTTGAAGTCTCTTGCACTCGAGGAGTGTGAATCTTTGGAATATCTGATTGACATGACACTTGTAAAACCTACACCACATAGTAGTGTCTTTCCTGTCTTGGATCGTTTGGTTGTATTTGGAGGAAGTAGGCTGAAGGAGATTTTTCATGGTGAGCTGCCAATGGGATCTCTTAAGAAACTACGTGAGATGGATTTACAATGTCTACCTTCATTGACCTATATTTGGAAGACTGAAAGTCATTCTGAATGCTTGAGAAATATTCTAGGACTCATTGAAGTCCTAAAAATAAAAAACTGCCCGTCGTTGGAAGAGATCGTTGCCTTGGAAGGATCAAGACATCAGGAGGAAGAAGCAACCAAAGAAATCAACTTCCAAAGATTGACAGAGTTGACTCTTGCAGATCTACCAAGTTTCACTGGGATAAGGCAAAATATCTATAAAAAGGAGTTGGAAAGGCTACCGGATGTTATCAGTCTCTATCCAAATGTTTTGACAGTGGAACATTCTAATGTGTCCATACAACATCTGTTTGACACCAAGGTTCAATTTCCAGTTATACGAAGACTAAAAATTGAAGGCATGGATAACTTAAAAGGGATATGGAACAGCGAACTTTCACCTGATTCCTTCTGTGAACTAAAATTTCTAATAGTGAACAGATGTAACAAACTATTGCATTTGGTGCCAACACATATGCAAAATAGATTGCAGAAAATGGAGTTCATATGTGCATCACACTGTTCTTCACTAGAAGAGATCTTTGATTTCAGGAGATCACCTTCACATATATCCCAACCTGGCGAAGGGATGCAAAGGAGCAAGATCATGGATTTCAAACAATCTTGTCAAGCCTTTCAGAATCTAACAGATATATCACTCTGGGAATGTAACAGTGTTAGAATTGTGCTCTCTCCTTCAATTTCCAAGAGTCTTGTGAAACTGAAAGTACTAAGGATATGGCGGTGCCAGAAGATTGAAGAAATAGTAGCAGCAGCAGAAGAGGGTGAAGAAACAGAGGATAACAACATGTTTCCTCAGCTGAGTAAATTGGTACTTGAGGAGCTACCAAATCTTCGAAGCTTTTCAGAAGGCAAATATAATTTTGAATGGCCACTGCTGAAATTTATATATATTGGAAAATGCTATATGAAGCATTTTTGTTTTGGATCCCTCAGCATTCCAAGGGAAGTGATTATATCTGTTAGTGATGCTGGTGAGAATCTTTTGCAGGAGCTCAATAACAGTAGAAAAGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1307

Amino Acids

148.59

Weight (kDa)

5.98

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATPase_2 PF01637 188 - 301 1e-06 ATPase domain predominantly from Archaea
NB-ARC PF00931 193 - 357 2.8e-21 NB-ARC domain
LRR_14 PF23598 609 - 706 4.8e-06 Leucine-rich repeat region
LRR_8 PF13855 640 - 695 7.8e-08 Leucine rich repeat
LRR_RPS2 PF23247 867 - 969 1.9e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1077 - 1147 3.5e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1167 - 1269 3.2e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 848
Acc36I ACCTGC 3 cut(s) 178, 848, 1793
AccB1I GGYRCC 4 cut(s) 491, 860, 3346, 3627
AccB7I CCANNNNNTGG 1 cut(s) 2337
AccI GTMKAC 3 cut(s) 228, 713, 2866
AccII CGCG 1 cut(s) 1533
AciI CCGC 5 cut(s) 64, 527, 1751, 2231, 3625
AclWI GGATC 9 cut(s) 191, 1085, 1409, 1954, 2772, 2836, 3001, 3825, 3838
AcoI YGGCCR 2 cut(s) 1643, 3773
AcsI RAATTY 9 cut(s) 431, 519, 1012, 1280, 1981, 2281, 2614, 3305, 3785
AcuI CTGAAG 3 cut(s) 1449, 1578, 2816
AdeI CACNNNGTG 1 cut(s) 2045
AfaI GTAC 5 cut(s) 89, 2264, 2560, 3612, 3717
AfiI CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2886, 2968, 3467
AflII CTTAAG 1 cut(s) 2834
AflIII ACRYGT 1 cut(s) 3690
AgeI ACCGGT 1 cut(s) 2407
AhdI GACNNNNNGTC 1 cut(s) 2898
AjnI CCWGG 5 cut(s) 199, 1046, 1246, 2041, 3460
AjuI GAANNNNNNNTTGG 4 cut(s) 1138, 1170, 1180, 1212
Alw21I GWGCWC 4 cut(s) 1713, 2307, 3573, 3900
Alw26I GTCTC 5 cut(s) 46, 263, 2159, 2676, 3143
AlwI GGATC 9 cut(s) 191, 1085, 1409, 1954, 2772, 2836, 3001, 3825, 3838
AlwNI CAGNNNCTG 1 cut(s) 130
Ama87I CYCGRG 2 cut(s) 392, 2681
AoxI GGCC 4 cut(s) 1044, 1643, 2322, 3773
ApeKI GCWGC 4 cut(s) 287, 2818, 3653, 3656
ApoI RAATTY 9 cut(s) 431, 519, 1012, 1280, 1981, 2281, 2614, 3305, 3785
ArsI GACNNNNNNTTYG 2 cut(s) 2654, 2686
AsiGI ACCGGT 1 cut(s) 2407
Asp700I GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3745
AspLEI GCGC 2 cut(s) 406, 1533
AspS9I GGNCC 1 cut(s) 2322
AsuII TTCGAA 1 cut(s) 3739
AvaI CYCGRG 2 cut(s) 392, 2681
BaeGI GKGCMC 1 cut(s) 1174
BalI TGGCCA 2 cut(s) 1645, 3775
BamHI GGATCC 1 cut(s) 3830
BanI GGYRCC 4 cut(s) 491, 860, 3346, 3627
BanII GRGCYC 2 cut(s) 1713, 3900
BarI GAAGNNNNNNTAC 2 cut(s) 2385, 2417
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 5 cut(s) 659, 788, 1070, 2897, 3232
Bbv12I GWGCWC 4 cut(s) 1713, 2307, 3573, 3900
BbvCI CCTCAGC 3 cut(s) 222, 3699, 3836
BbvI GCAGC 4 cut(s) 299, 2805, 3665, 3668
BccI CCATC 5 cut(s) 658, 1028, 1837, 2466, 2845
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 5 cut(s) 201, 1048, 1248, 2043, 3462
BciVI GTATCC 1 cut(s) 2343
BclI TGATCA 1 cut(s) 940
BcoDI GTCTC 5 cut(s) 46, 263, 2159, 2676, 3143
BfaI CTAG 9 cut(s) 17, 335, 791, 2013, 2069, 2120, 2252, 2927, 3413
BfmI CTRYAG 7 cut(s) 174, 273, 399, 537, 855, 1678, 2466
BfrI CTTAAG 1 cut(s) 2834
BfuAI ACCTGC 3 cut(s) 178, 848, 1793
BfuI GTATCC 1 cut(s) 2343
BglII AGATCT 5 cut(s) 388, 1780, 2460, 3064, 3420
BisI GCNGC 7 cut(s) 65, 288, 527, 1751, 2819, 3654, 3657
BlsI GCNGC 7 cut(s) 66, 289, 528, 1752, 2820, 3655, 3658
BmcAI AGTACT 2 cut(s) 2560, 3612
Bme1390I CCNGG 5 cut(s) 201, 1048, 1248, 2043, 3462
BmeRI GACNNNNNGTC 1 cut(s) 2898
BmeT110I CYCGRG 2 cut(s) 392, 2681
BmgT120I GGNCC 1 cut(s) 2322
BmiI GGNNCC 6 cut(s) 493, 862, 1749, 3348, 3629, 3832
BmrFI CCNGG 5 cut(s) 201, 1048, 1248, 2043, 3462
BmrI ACTGGG 1 cut(s) 3090
BmsI GCATC 8 cut(s) 115, 415, 811, 1715, 2530, 3404, 3462, 3862
BmuI ACTGGG 1 cut(s) 3090
BoxI GACNNNNGTC 1 cut(s) 759
BpiI GAAGAC 5 cut(s) 659, 788, 1070, 2897, 3232
BplI GAGNNNNNCTC 2 cut(s) 2021, 2053
BpmI CTGGAG 3 cut(s) 222, 1069, 2222
Bpu10I CCTNAGC 3 cut(s) 222, 3699, 3836
Bpu14I TTCGAA 1 cut(s) 3739
BpuEI CTTGAG 5 cut(s) 1769, 2048, 2542, 2935, 3740
BsaAI YACGTR 2 cut(s) 1943, 2846
BsaBI GATNNNNATC 1 cut(s) 1878
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 5 cut(s) 1614, 2331, 2985, 3201, 3845
BsaWI WCCGGW 3 cut(s) 619, 2407, 3124
Bsc4I CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2886, 2968, 3467
Bse118I RCCGGY 1 cut(s) 2407
Bse1I ACTGG 7 cut(s) 1838, 2205, 2342, 2376, 2566, 3085, 3215
Bse8I GATNNNNATC 1 cut(s) 1878
BseBI CCWGG 5 cut(s) 201, 1048, 1248, 2043, 3462
BseDI CCNNGG 5 cut(s) 1614, 2331, 2985, 3201, 3845
BseGI GGATG 4 cut(s) 406, 2477, 3133, 3477
BseJI GATNNNNATC 1 cut(s) 1878
BseLI CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2886, 2968, 3467
BseMII CTCAG 9 cut(s) 213, 444, 479, 516, 1948, 2154, 3695, 3713, 3850
BseNI ACTGG 7 cut(s) 1838, 2205, 2342, 2376, 2566, 3085, 3215
BseRI GAGGAG 6 cut(s) 368, 490, 580, 2315, 2699, 3737
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 4 cut(s) 299, 2805, 3665, 3668
BsgI GTGCAG 1 cut(s) 1155
Bsh1236I CGCG 1 cut(s) 1533
BshFI GGCC 4 cut(s) 1046, 1645, 2324, 3775
BshNI GGYRCC 4 cut(s) 491, 860, 3346, 3627
BshTI ACCGGT 1 cut(s) 2407
BsiHKAI GWGCWC 4 cut(s) 1713, 2307, 3573, 3900
BsiHKCI CYCGRG 2 cut(s) 392, 2681
BsiSI CCGG 3 cut(s) 620, 2408, 3125
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2886, 2968, 3467
BsmAI GTCTC 5 cut(s) 46, 263, 2159, 2676, 3143
BsmFI GGGAC 1 cut(s) 409
BsmI GAATGC 4 cut(s) 1210, 1521, 2915, 3840
BsnI GGCC 4 cut(s) 1046, 1645, 2324, 3775
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 2 cut(s) 392, 2681
Bsp119I TTCGAA 1 cut(s) 3739
Bsp1286I GDGCHC 5 cut(s) 1174, 1713, 2307, 3573, 3900
Bsp1407I TGTACA 1 cut(s) 2262
Bsp19I CCATGG 1 cut(s) 2331
BspACI CCGC 5 cut(s) 64, 527, 1751, 2231, 3625
BspANI GGCC 4 cut(s) 1046, 1645, 2324, 3775
BspCNI CTCAG 9 cut(s) 214, 445, 478, 515, 1947, 2155, 3696, 3712, 3849
BspFNI CGCG 1 cut(s) 1533
BspHI TCATGA 2 cut(s) 1926, 2530
BspLI GGNNCC 6 cut(s) 493, 862, 1749, 3348, 3629, 3832
BspMAI CTGCAG 2 cut(s) 859, 1682
BspMI ACCTGC 3 cut(s) 178, 848, 1793
BspPI GGATC 9 cut(s) 191, 1085, 1409, 1954, 2772, 2836, 3001, 3825, 3838
BspQI GCTCTTC 1 cut(s) 420
BspT104I TTCGAA 1 cut(s) 3739
BspT107I GGYRCC 4 cut(s) 491, 860, 3346, 3627
BspTI CTTAAG 1 cut(s) 2834
BspTNI GGTCTC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 2407
BsrGI TGTACA 1 cut(s) 2262
BsrI ACTGG 7 cut(s) 1838, 2205, 2342, 2376, 2566, 3085, 3215
BssAI RCCGGY 1 cut(s) 2407
BssECI CCNNGG 5 cut(s) 1614, 2331, 2985, 3201, 3845
BssNAI GTATAC 1 cut(s) 714
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 4 cut(s) 2331, 2985, 3201, 3845
Bst1107I GTATAC 1 cut(s) 714
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 5 cut(s) 201, 1048, 1248, 2043, 3462
Bst6I CTCTTC 7 cut(s) 261, 420, 2067, 2207, 2967, 3411, 3657
BstAFI CTTAAG 1 cut(s) 2834
BstAPI GCANNNNNTGC 1 cut(s) 3346
BstAUI TGTACA 1 cut(s) 2262
BstBAI YACGTR 2 cut(s) 1943, 2846
BstBI TTCGAA 1 cut(s) 3739
BstDSI CCRYGG 1 cut(s) 2331
BstEII GGTNACC 1 cut(s) 2422
BstENI CCTNNNNNAGG 1 cut(s) 3465
BstF5I GGATG 4 cut(s) 406, 2477, 3133, 3477
BstFNI CGCG 1 cut(s) 1533
BstHHI GCGC 2 cut(s) 406, 1533
BstMAI GTCTC 5 cut(s) 46, 263, 2159, 2676, 3143
BstMWI GCNNNNNNNGC 5 cut(s) 179, 284, 410, 1708, 3346
BstNI CCWGG 5 cut(s) 201, 1048, 1248, 2043, 3462
BstNSI RCATGY 2 cut(s) 294, 3694
BstPAI GACNNNNGTC 1 cut(s) 759
BstPI GGTNACC 1 cut(s) 2422
BstSCI CCNGG 5 cut(s) 199, 1046, 1246, 2041, 3460
BstSFI CTRYAG 7 cut(s) 174, 273, 399, 537, 855, 1678, 2466
BstSLI GKGCMC 1 cut(s) 1174
BstUI CGCG 1 cut(s) 1533
BstV1I GCAGC 4 cut(s) 299, 2805, 3665, 3668
BstV2I GAAGAC 5 cut(s) 659, 788, 1070, 2897, 3232
BstX2I RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2828, 3064, 3420, 3830
BstXI CCANNNNNNTGG 1 cut(s) 2332
BstYI RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2828, 3064, 3420, 3830
BstZ17I GTATAC 1 cut(s) 714
BsuI GTATCC 1 cut(s) 2343
BsuRI GGCC 4 cut(s) 1046, 1645, 2324, 3775
BtgI CCRYGG 1 cut(s) 2331
BtgZI GCGATG 1 cut(s) 1548
BtsCI GGATG 4 cut(s) 406, 2477, 3133, 3477
BtsI GCAGTG 1 cut(s) 3776
BtsIMutI CAGTG 8 cut(s) 1030, 1498, 1685, 3078, 3165, 3400, 3562, 3776
BveI ACCTGC 3 cut(s) 178, 848, 1793
CaiI CAGNNNCTG 1 cut(s) 130
CciI TCATGA 2 cut(s) 1926, 2530
CfoI GCGC 2 cut(s) 406, 1533
Cfr10I RCCGGY 1 cut(s) 2407
Cfr13I GGNCC 1 cut(s) 2322
CsiI ACCWGGT 1 cut(s) 2041
Csp6I GTAC 5 cut(s) 88, 2263, 2559, 3611, 3716
CspAI ACCGGT 1 cut(s) 2407
CviQI GTAC 5 cut(s) 88, 2263, 2559, 3611, 3716
DraI TTTAAA 2 cut(s) 1408, 1978
DraIII CACNNNGTG 1 cut(s) 2045
DriI GACNNNNNGTC 1 cut(s) 2898
EaeI YGGCCR 2 cut(s) 1643, 3773
Eam1104I CTCTTC 7 cut(s) 261, 420, 2067, 2207, 2967, 3411, 3657
Eam1105I GACNNNNNGTC 1 cut(s) 2898
EarI CTCTTC 7 cut(s) 261, 420, 2067, 2207, 2967, 3411, 3657
Ecl136II GAGCTC 2 cut(s) 1711, 3898
Eco130I CCWWGG 4 cut(s) 2331, 2985, 3201, 3845
Eco147I AGGCCT 1 cut(s) 1046
Eco24I GRGCYC 2 cut(s) 1713, 3900
Eco31I GGTCTC 1 cut(s) 46
Eco53kI GAGCTC 2 cut(s) 1711, 3898
Eco57I CTGAAG 3 cut(s) 1449, 1578, 2816
Eco88I CYCGRG 2 cut(s) 392, 2681
Eco91I GGTNACC 1 cut(s) 2422
EcoICRI GAGCTC 2 cut(s) 1711, 3898
EcoNI CCTNNNNNAGG 1 cut(s) 3465
EcoO65I GGTNACC 1 cut(s) 2422
EcoRII CCWGG 5 cut(s) 199, 1046, 1246, 2041, 3460
EcoT14I CCWWGG 4 cut(s) 2331, 2985, 3201, 3845
EcoT22I ATGCAT 2 cut(s) 1521, 1730
EcoT38I GRGCYC 2 cut(s) 1713, 3900
ErhI CCWWGG 4 cut(s) 2331, 2985, 3201, 3845
FalI AAGNNNNNCTT 4 cut(s) 723, 755, 762, 794
FaqI GGGAC 1 cut(s) 409
FauNDI CATATG 3 cut(s) 629, 3357, 3389
FbaI TGATCA 1 cut(s) 940
FblI GTMKAC 3 cut(s) 228, 713, 2866
Fnu4HI GCNGC 7 cut(s) 65, 288, 527, 1751, 2819, 3654, 3657
FokI GGATG 4 cut(s) 393, 2484, 3140, 3484
FriOI GRGCYC 2 cut(s) 1713, 3900
Fsp4HI GCNGC 7 cut(s) 65, 288, 527, 1751, 2819, 3654, 3657
FspBI CTAG 9 cut(s) 17, 335, 791, 2013, 2069, 2120, 2252, 2927, 3413
GlaI GCGC 2 cut(s) 405, 1532
GluI GCNGC 7 cut(s) 65, 288, 527, 1751, 2819, 3654, 3657
GsuI CTGGAG 3 cut(s) 222, 1069, 2222
HaeIII GGCC 4 cut(s) 1046, 1645, 2324, 3775
HapII CCGG 3 cut(s) 620, 2408, 3125
HhaI GCGC 2 cut(s) 406, 1533
Hin6I GCGC 2 cut(s) 404, 1531
HinP1I GCGC 2 cut(s) 404, 1531
HincII GTYRAC 3 cut(s) 679, 1566, 3054
HindII GTYRAC 3 cut(s) 679, 1566, 3054
HindIII AAGCTT 4 cut(s) 180, 956, 2444, 3742
HpaI GTTAAC 1 cut(s) 679
HpaII CCGG 3 cut(s) 620, 2408, 3125
Hpy99I CGWCG 1 cut(s) 2968
HpyCH4IV ACGT 3 cut(s) 841, 1942, 2845
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 284, 410, 1708, 3346
HpySE526I ACGT 3 cut(s) 841, 1942, 2845
HspAI GCGC 2 cut(s) 404, 1531
Ksp22I TGATCA 1 cut(s) 940
KspAI GTTAAC 1 cut(s) 679
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 9 cut(s) 503, 1708, 1747, 2302, 2333, 2479, 3480, 3724, 3895
Lsp1109I GCAGC 4 cut(s) 299, 2805, 3665, 3668
LweI GCATC 8 cut(s) 115, 415, 811, 1715, 2530, 3404, 3462, 3862
MabI ACCWGGT 1 cut(s) 2041
MaeI CTAG 9 cut(s) 17, 335, 791, 2013, 2069, 2120, 2252, 2927, 3413
MaeII ACGT 3 cut(s) 841, 1942, 2845
MaeIII GTNAC 4 cut(s) 964, 2422, 3326, 3551
MfeI CAATTG 3 cut(s) 1146, 2140, 2153
MflI RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2828, 3064, 3420, 3830
MhlI GDGCHC 5 cut(s) 1174, 1713, 2307, 3573, 3900
MlsI TGGCCA 2 cut(s) 1645, 3775
MluNI TGGCCA 2 cut(s) 1645, 3775
MlyI GAGTC 4 cut(s) 1561, 2925, 3049, 3601
MmeI TCCRAC 4 cut(s) 1144, 1467, 2949, 3093
Mox20I TGGCCA 2 cut(s) 1645, 3775
Mph1103I ATGCAT 2 cut(s) 1521, 1730
MroXI GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3745
MscI TGGCCA 2 cut(s) 1645, 3775
MslI CAYNNNNRTG 4 cut(s) 161, 1266, 2745, 3171
Msp20I TGGCCA 2 cut(s) 1645, 3775
MspA1I CMGCKG 1 cut(s) 3703
MspCI CTTAAG 1 cut(s) 2834
MspI CCGG 3 cut(s) 620, 2408, 3125
MspR9I CCNGG 5 cut(s) 201, 1048, 1248, 2043, 3462
MunI CAATTG 3 cut(s) 1146, 2140, 2153
Mva1269I GAATGC 4 cut(s) 1210, 1521, 2915, 3840
MvaI CCWGG 5 cut(s) 201, 1048, 1248, 2043, 3462
MvnI CGCG 1 cut(s) 1533
MwoI GCNNNNNNNGC 5 cut(s) 179, 284, 410, 1708, 3346
NcoI CCATGG 1 cut(s) 2331
NdeI CATATG 3 cut(s) 629, 3357, 3389
NlaIV GGNNCC 6 cut(s) 493, 862, 1749, 3348, 3629, 3832
NsiI ATGCAT 2 cut(s) 1521, 1730
NspI RCATGY 2 cut(s) 294, 3694
NspV TTCGAA 1 cut(s) 3739
PaeI GCATGC 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 2681
PagI TCATGA 2 cut(s) 1926, 2530
PaqCI CACCTGC 1 cut(s) 848
PceI AGGCCT 1 cut(s) 1046
PciI ACATGT 1 cut(s) 3690
PciSI GCTCTTC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PctI GAATGC 4 cut(s) 1210, 1521, 2915, 3840
PdmI GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3745
PflMI CCANNNNNTGG 1 cut(s) 2337
PfoI TCCNGGA 2 cut(s) 199, 1246
PinAI ACCGGT 1 cut(s) 2407
PkrI GCNGC 7 cut(s) 66, 289, 528, 1752, 2820, 3655, 3658
PleI GAGTC 4 cut(s) 1561, 2925, 3049, 3600
PpsI GAGTC 4 cut(s) 1561, 2925, 3049, 3600
Ppu21I YACGTR 2 cut(s) 1943, 2846
PscI ACATGT 1 cut(s) 3690
PshAI GACNNNNGTC 1 cut(s) 759
Psp124BI GAGCTC 2 cut(s) 1713, 3900
Psp6I CCWGG 5 cut(s) 199, 1046, 1246, 2041, 3460
PspEI GGTNACC 1 cut(s) 2422
PspGI CCWGG 5 cut(s) 199, 1046, 1246, 2041, 3460
PspN4I GGNNCC 6 cut(s) 493, 862, 1749, 3348, 3629, 3832
PspPI GGNCC 1 cut(s) 2322
PspXI VCTCGAGB 1 cut(s) 2681
PstI CTGCAG 2 cut(s) 859, 1682
PstNI CAGNNNCTG 1 cut(s) 130
PsuI RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2828, 3064, 3420, 3830
PvuII CAGCTG 1 cut(s) 3703
RsaI GTAC 5 cut(s) 89, 2264, 2560, 3612, 3717
RsaNI GTAC 5 cut(s) 88, 2263, 2559, 3611, 3716
RseI CAYNNNNRTG 4 cut(s) 161, 1266, 2745, 3171
SacI GAGCTC 2 cut(s) 1713, 3900
SapI GCTCTTC 1 cut(s) 420
SatI GCNGC 7 cut(s) 65, 288, 527, 1751, 2819, 3654, 3657
Sau96I GGNCC 1 cut(s) 2322
ScaI AGTACT 2 cut(s) 2560, 3612
SchI GAGTC 4 cut(s) 1561, 2925, 3049, 3601
ScrFI CCNGG 5 cut(s) 201, 1048, 1248, 2043, 3462
SduI GDGCHC 5 cut(s) 1174, 1713, 2307, 3573, 3900
SexAI ACCWGGT 1 cut(s) 2041
SfaNI GCATC 8 cut(s) 115, 415, 811, 1715, 2530, 3404, 3462, 3862
SfcI CTRYAG 7 cut(s) 174, 273, 399, 537, 855, 1678, 2466
Sfr274I CTCGAG 1 cut(s) 2681
SfuI TTCGAA 1 cut(s) 3739
SlaI CTCGAG 1 cut(s) 2681
SmiMI CAYNNNNRTG 4 cut(s) 161, 1266, 2745, 3171
SmlI CTYRAG 7 cut(s) 1784, 2027, 2521, 2681, 2834, 2914, 3719
SmoI CTYRAG 7 cut(s) 1784, 2027, 2521, 2681, 2834, 2914, 3719
SphI GCATGC 1 cut(s) 294
SseBI AGGCCT 1 cut(s) 1046
SsiI CCGC 5 cut(s) 64, 527, 1751, 2231, 3625
SspI AATATT 5 cut(s) 449, 550, 882, 1373, 2923
SspMI CTAG 9 cut(s) 17, 335, 791, 2013, 2069, 2120, 2252, 2927, 3413
SstI GAGCTC 2 cut(s) 1713, 3900
StuI AGGCCT 1 cut(s) 1046
StyD4I CCNGG 5 cut(s) 199, 1046, 1246, 2041, 3460
StyI CCWWGG 4 cut(s) 2331, 2985, 3201, 3845
TaiI ACGT 3 cut(s) 844, 1945, 2848
TaqI TCGA 5 cut(s) 563, 572, 896, 2682, 3739
TatI WGTACW 4 cut(s) 87, 2262, 2558, 3610
TauI GCSGC 3 cut(s) 67, 529, 1753
TscAI CASTG 8 cut(s) 1030, 1498, 1692, 3085, 3165, 3407, 3562, 3783
TseI GCWGC 4 cut(s) 287, 2818, 3653, 3656
TspRI CASTG 8 cut(s) 1030, 1498, 1692, 3085, 3165, 3407, 3562, 3783
Van91I CCANNNNNTGG 1 cut(s) 2337
Vha464I CTTAAG 1 cut(s) 2834
XagI CCTNNNNNAGG 1 cut(s) 3465
XapI RAATTY 9 cut(s) 431, 519, 1012, 1280, 1981, 2281, 2614, 3305, 3785
XbaI TCTAGA 1 cut(s) 2012
XceI RCATGY 2 cut(s) 294, 3694
XcmI CCANNNNNNNNNTGG 2 cut(s) 915, 1102
XhoI CTCGAG 1 cut(s) 2681
XmiI GTMKAC 3 cut(s) 228, 713, 2866
XmnI GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3745
XspI CTAG 9 cut(s) 17, 335, 791, 2013, 2069, 2120, 2252, 2927, 3413
ZrmI AGTACT 2 cut(s) 2560, 3612
Zsp2I ATGCAT 2 cut(s) 1521, 1730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.