Rw6G022330

ADP binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000203
Physical Location & Seq
Reverse (-)
44167701 .. 44176713
9013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G022330.1

Sequence Viewer

Length: 4662 bp
ATGGCAGAAATATGTATTTCAGTTGTGGCCAAATTAGCGGAGTACACAGTCGCTCCTGTTCTGCGACCGTTTGGTTATCTGATTAACTACAAGAGCAATGTTCAAGATCTCACCGAGAAGGTTAGAACTTTGACTGCCAAAAGAGATGGGGTACTACGACGTGTAGATGCAGCGAAGAGGAACTTGGAGGGTATTCGTCCCGAAGTTGAGCTCTGGTTGGAGAGAGTAGACCAAACCATACAAGAAAAGGAGACATGTTTTGAAGAAGGAAGAGTAACCGAGGCAACAACATGCTGCAGCTCCAATGGGTGGTGTCCAAATCTGAAGGTCCGTTATTCCTTGAGTAGGACAGCAACGAGGATGGTTCCAAGGGTTGATGACCTTATTACAGGTGGAAATTTCACCACAATATCATGTCCTCCTCCTCCGCTGAAGATTGAATATCCAGCCATACCATATATTGAGGATTCCACTGAGGCACTGGCCAAGCCACATGAAGGGGCTAGCTCCTCCTCAGGAACATCCGATCCTGCTAAACCACCTGCACCTATGGATCTCTCTGAGCGTCTGAAATATAGGTTGCCTTATACAAAGGATCTCTTGACGGCTCTGCAAGATGACAAGATCAGCATGATCGGCATTTCTGGGACAATCAGAGTGATAGATACAGTAACAACTAAAGAGTTCATGAAAAGAATGAAACAGAAAAAGCTCTTTGAAGAGGTTGCGATGGCAGTTGTGTCCCAGGGTCCAGACTTGAAACGAATACAAGGTGAAATTGCAGAAATGCTAGACCTGAAATTGGAGAATGGGAGTTTGGTTGAACGGGCAGAGAGGCTACGTGCTGTTTTACAGGCATCCGATTCTAAGAGGATTCTTGTAATACTGACTGATATCTGTGAAGTACCTGATTTGGAAGCAATTGGAATTTCTCGTGTTGATATCAAGAAAAGCTGCAAGATCATGTTGATTTCACAACTATCAAATGTGTTTGGTGAAATGGGGACTCAGAGCAATTTCAAATTGTTATTACAATCCACTCCCAGAGCAAGACCTGTTGAGTTTAGATCAAGGATCTCAGTCATCAGAGATGTAATGGATGCTGTGACAGATGATGAATCCAACCCCATTGTAATATGTGGGATGGGGGGAGTTGGCAAAACAACCTTGGTGATGGAAATTGGTGCAAAAGCGAGTGAAGTAGATCTATTTGATGATGTTGCAATTGCAGAGTTTACTCAAGAACCTGACTTGATCAAAATCCAAGGAAAAATTGCTCAAGCTCTAGGCCTGAAACTTGAAGCAGAGGATGACCGAGCAGCCAAGTTACGAGAAAGATTATCAATGGATACGAAAAAGGTCCTTGTAATATTGGACAATGTTTGGGGACAACTAGATCTGTGGGAACTAGGAATTCCAATTTCCCGGGATCCAAAAAGTTGCAAACTTCTGGTATCATCACGGAATCAAGATATCTTTAAAGATATGAAAACCAAAAAGAACTTCCTCATTGGTAGTTTGCTAAAACATGATGCCTGGAGTTTGTTTAAGAAGGTAGCAGGATGTTCCATTGAATCCGATTCCGAGCTGCGTCCTATAGCAGAGCAGGTTCTGGATGAATGTGATGGCTTACCGGTTGCAATTTCTACTGTTGGAAGGGCACTACAAGATGAAAAAATTCCAGTATGGAGAAATGCACTTAGACAACTAAGAATGGCTAGCCCAAAAGATGTTCCAGGAGTTATAGAGCGTGTGTACGGAAAAATAGAGTTCAGTTATGAATGTCTACCGAGCGAGGAAGCCAAATCATGTTTTTTGCTGTGTTGCATATTTCCAGAAAGTGAAGAGTTTCCAATTGAATGGTTGGTTATGTTTGCGATTGGGCTTGGACTTTTTAAAGACATTGATTCAATATCTGGAGCAAGGGATTACGTAATAACAGTGGTTGAAACACTCAAATGTCGTTCTCTGTTGTTAGACGGCGATGAGAAAGATTGTTTTAGAATGCATGATGTGGTGCGTGACGTAGCCGTGTATATAGCCTCCAAAGAGTTAGCACAAAAGAAGAGTGATAAGCTTGAAAGGAGATTTATGGAAAGAGGAGGAGTGGAATTGAACGATTGGCTCAAGAGCAGTTCACTGAGAGCCGATATGAGTACTACTGAGCTTTTAGTGTTGGCAGCCACAGATGGAATTGAACTGGAGCTGCCAGCCACAATTTTCGATGGAACGGAAAATCTCAAGGTTTTATTGATCTGGCGTTCAATCCTCCCATCCCTTTCACTTCTGAAGAATCTTCAAACACTCATTCTAGTGGATGTCTGTCGCAATATTGATGTCATTGGAGAGCTACGGTCACTCATGATACTCTGTTTCCTCGAATGTGACGACGTCAAACAGTTGCCTGATACATTCAAAAATTTGTCTAATCTGAGGTTGTTAAACTTGACAGGATGTACAGAGCTTAAAGTAATCTCACCAGGTGTTATATCGAGTTTATCTCGTCTAGAAGAATTGTACATGGGGAATGGCTTTCAAGATTGGGTGGTTGAGAAACTGGGCTCAACAGAAACAATCAATTGGATCTCTCAATTGAAGGTTGATGCCCAACCAGAAGAAGTCAACATGTGGAAGAAATTCAACAGGTTGGCGGTTGAGAAACTAGCCTCAACAGAAACAACAACTGATCAGGTCTCTCAACGGGAGGAGGATGAGGAAAGAAGTGAACCAGAGGAACTCGACAGCCTTGGACATCTGTTCGCAGAGTATGTTGAAGATTTAGAAAACTACGACCATCTCACAAGTTGGGCCAACTCTATGGACTGGAGAGTTGGATTACTTTCAGAGCTACTTTCCTTGTCCCGTTTGACTACTTTAGAAGTGGTTTTACCACCAATTGAGATTCTGCGAACCAGTAAGCTATTTCATAAGCTTGAAAGATTTAAGATCTCTATTGGATGGGATGAGCATGAAGACTTGGAGGACTTAAAGGGTAACTACTTGAGAGTTCATGATCTTGAAGCAAGCTCTCTTGCGGATACTGGAATCGGTTTGTTGTTAAAGAAAACTACCAAGCTTGAATTAAAAATGAAGAATTTGACTGACCCTCTCAATGTCTTAGATGTAAACTGCTGTGCAAACTTGGAGTCACTTACCCTCAAAGACTGTGATTCTCTGGAATATCTGATTGACATGACTCTTAAGCAAGATACTCCACGAAGCGTCTTTCCTGTCTTGAATTATCTGGAAATCAATGGAGCAAGGAGGCTGAAAGAGATTTTTCATGGTGACCTGCCACTTGGATCGCTTCAGAAACTAGAAAGGCTAACTTTACAGGATTGCCAGTCATTGGAAGCAATTTTTGCCTTGGAAGGATCGGAACATCATGTCAAAGAGATCAACTTCCCTAGTTTGACAAAGTTGGAACTTAAAGGTCTCCCGAGCTTCACTTTCCAGTCATTGGATGTAAACTGCTGTGCAAACTTGGAGTCACTTACCCTCAAAGACTGTGATTCTCTGGAATATCTGATTGACATGACTCTTAAGCAAGATACTCCACGAAGCGTCTTTCCTGTCTTGAATTATCTGGAAATCAATGGAGCAAGCAGGTTGAAAGGGATTTTTCATGGTGACCTGCCACTGGAGTCGCTTCAGAAACTAGAAGAGCTAACTTTACAGGATTGCCAGTCATTGGAAGCAATTTTTTCCATGGAAGGATCGGGACATCATGTCAAAGAGATCAACTTCCTTAGTTTGACACGGTTGATACTTGAAGGTCTACCAAGTTTCACTGGAATGAGCAAAAGTACTTGTAAGGGGCTGGAAAAGCAACTGGAAGTCGGCAGTCTCCATCCCAATAGCTTGACCGCTGAACATTCACTTTTCTCTGACCCCAAGGTTGTCTTTCCCGTCTTGAGAGACCTGGACATTAGCCGTCTGGGCAACTTGAAAGAGATATGGAATAGCCGACTTTCACCCAACTCCTTCTCTGAATTAAGAACACTCACTGTCGAGGATTGTGACAAACTATTGCATTTGGTACCAACACAAATGCAAAATAGATTGCAGAGATTGGAGACCATACGGGTAGAGAACTGTTCTTCACTGGAAGAGATTTTTGAAGTCGGAAGATTGACCGTCAATGAAGGGAATGCTTCAATTTCTCAGTCAGACAAAATACCTTCAAATATTTCCCAACCTGATCAAGAGATGCAAATAAATGATATCATGGATTTCAAACAATCTTGTCAAGGCTTTCAGAATTTAACCCAACTAGAAGTTAGTTCTTGTGGCAGTTTGAGATACCTGCTCTCTCCTTCGATTGCCAGAGGTCTTGTCAAGCTCAAACGTCTAAATATAAGATCTTGCAAGATGATCGAAGCAATAGTCGCAGCAGATGAGGGTGAAGAAACAGAGAATGAAAGCATGCTCCCTCAGCTTTTTGATTTGTATCTTGGGGATCTACCCAATCTTGGAAGCTTTTCGCAAGGCAAATATACTTTTGATTGGCCACGAGTGAGATGGATCGAAATTAACAAATGCAACAAAATGAACAACTTTTGTTCAGGATCCCTCAGCATCCCAAGGGAAGTGAGCATAGATGTCTCTAATTCTGGTGAGAATCTTGAGCAGGAGCTGAATGATAGCAAGAAAGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1553

Amino Acids

174.64

Weight (kDa)

4.99

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 203 - 338 1.1e-10 NB-ARC domain
NB-ARC PF00931 365 - 520 1.8e-22 NB-ARC domain
LRR_RPS2 PF23247 1071 - 1182 4.5e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1185 - 1264 6.5e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1307 - 1457 1.1e-28 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1409 - 1520 3.1e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 550
AatII GACGTC 1 cut(s) 2394
Acc36I ACCTGC 6 cut(s) 550, 1597, 3300, 3597, 3642, 4314
Acc65I GGTACC 1 cut(s) 4039
AccB1I GGYRCC 1 cut(s) 4039
AccB7I CCANNNNNTGG 4 cut(s) 309, 3349, 3460, 3691
AccI GTMKAC 3 cut(s) 228, 1786, 3778
AciI CCGC 5 cut(s) 38, 428, 2651, 3035, 3867
AcoI YGGCCR 3 cut(s) 27, 483, 4508
AcsI RAATTY 8 cut(s) 397, 927, 1413, 1677, 2419, 2636, 3094, 4261
AcuI CTGAAG 5 cut(s) 344, 452, 2309, 3293, 3635
AcyI GRCGYC 1 cut(s) 2391
AdeI CACNNNGTG 1 cut(s) 2483
AfaI GTAC 9 cut(s) 44, 153, 906, 1757, 2158, 2458, 2519, 3808, 4041
AfiI CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 3349, 3460, 3640, 3691, 4472
AflII CTTAAG 2 cut(s) 3200, 3542
AflIII ACRYGT 3 cut(s) 160, 254, 2625
AgeI ACCGGT 1 cut(s) 1633
AhdI GACNNNNNGTC 1 cut(s) 3728
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 5 cut(s) 744, 1535, 1735, 2479, 3921
AjuI GAANNNNNNNTTGG 6 cut(s) 167, 199, 800, 832, 1487, 1519
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 7 cut(s) 245, 2698, 3440, 3851, 3912, 4070, 4609
AlwNI CAGNNNCTG 2 cut(s) 1612, 4636
Ama87I CYCGRG 2 cut(s) 1425, 3439
AoxI GGCC 5 cut(s) 27, 483, 1288, 2808, 4508
ApeKI GCWGC 9 cut(s) 170, 294, 297, 954, 1319, 1588, 2180, 2206, 4391
ApoI RAATTY 8 cut(s) 397, 927, 1413, 1677, 2419, 2636, 3094, 4261
ArsI GACNNNNNNTTYG 6 cut(s) 3131, 3163, 3473, 3505, 4371, 4403
AsiGI ACCGGT 1 cut(s) 1633
Asp700I GAANNNNTTC 4 cut(s) 1677, 1848, 2636, 4480
Asp718I GGTACC 1 cut(s) 4039
AspS9I GGNCC 4 cut(s) 328, 749, 1360, 2808
AsuC2I CCSGG 2 cut(s) 1426, 1427
AsuNHI GCTAGC 2 cut(s) 503, 1718
AvaI CYCGRG 2 cut(s) 1425, 3439
AvaII GGWCC 3 cut(s) 328, 749, 1360
AxyI CCTNAGG 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 1663
BalI TGGCCA 3 cut(s) 29, 485, 4510
BamHI GGATCC 2 cut(s) 1429, 4568
BanI GGYRCC 1 cut(s) 4039
BanII GRGCYC 2 cut(s) 213, 2564
BarI GAAGNNNNNNTAC 2 cut(s) 2871, 2903
BauI CACGAG 2 cut(s) 933, 4512
BbsI GAAGAC 1 cut(s) 2979
Bbv12I GWGCWC 1 cut(s) 213
BbvCI CCTCAGC 2 cut(s) 4434, 4574
BbvI GCAGC 9 cut(s) 182, 281, 309, 941, 1331, 1575, 2192, 2193, 4403
BceAI ACGGC 4 cut(s) 621, 1996, 2015, 3918
BcgI CGANNNNNNTGC 2 cut(s) 4357, 4391
BciT130I CCWGG 5 cut(s) 746, 1537, 1737, 2481, 3923
BciVI GTATCC 2 cut(s) 1342, 3031
BclI TGATCA 3 cut(s) 1254, 2686, 4201
BcnI CCSGG 2 cut(s) 1426, 1427
BcoDI GTCTC 7 cut(s) 245, 2698, 3440, 3851, 3912, 4070, 4609
BfmI CTRYAG 2 cut(s) 295, 1596
BfrI CTTAAG 2 cut(s) 3200, 3542
BfuAI ACCTGC 6 cut(s) 550, 1597, 3300, 3597, 3642, 4314
BfuI GTATCC 2 cut(s) 1342, 3031
BglI GCCNNNNNGGC 1 cut(s) 3939
BglII AGATCT 5 cut(s) 106, 1204, 1396, 2946, 4361
BisI GCNGC 9 cut(s) 171, 295, 298, 955, 1320, 1589, 2181, 2207, 4392
BlsI GCNGC 9 cut(s) 172, 296, 299, 956, 1321, 1590, 2182, 2208, 4393
BmcAI AGTACT 2 cut(s) 2158, 3808
Bme1390I CCNGG 7 cut(s) 746, 1426, 1427, 1537, 1737, 2481, 3923
Bme18I GGWCC 3 cut(s) 328, 749, 1360
BmeRI GACNNNNNGTC 1 cut(s) 3728
BmeT110I CYCGRG 2 cut(s) 1425, 3439
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 4 cut(s) 328, 749, 1360, 2808
BmiI GGNNCC 5 cut(s) 366, 750, 1431, 4041, 4570
BmrFI CCNGG 7 cut(s) 746, 1426, 1427, 1537, 1737, 2481, 3923
BmrI ACTGGG 1 cut(s) 2567
BmsI GCATC 7 cut(s) 157, 866, 1090, 1522, 2593, 4200, 4587
BmtI GCTAGC 2 cut(s) 507, 1722
BmuI ACTGGG 1 cut(s) 2567
BpiI GAAGAC 1 cut(s) 2979
BplI GAGNNNNNCTC 2 cut(s) 2485, 2517
BpmI CTGGAG 5 cut(s) 1558, 1938, 2222, 2845, 3662
Bpu10I CCTNAGC 2 cut(s) 4434, 4574
BpuEI CTTGAG 8 cut(s) 361, 1224, 1263, 2111, 2225, 3022, 3934, 4646
BpuMI CCSGG 2 cut(s) 1426, 1427
BsaAI YACGTR 2 cut(s) 842, 1933
BsaBI GATNNNNATC 2 cut(s) 1259, 4449
BsaHI GRCGYC 1 cut(s) 2391
BsaI GGTCTC 4 cut(s) 2698, 3440, 3912, 4070
BsaWI WCCGGW 1 cut(s) 1633
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bsc4I CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 3349, 3460, 3640, 3691, 4472
Bse118I RCCGGY 1 cut(s) 1633
Bse21I CCTNAGG 1 cut(s) 514
Bse3DI GCAATG 1 cut(s) 103
Bse8I GATNNNNATC 2 cut(s) 1259, 4449
BseBI CCWGG 5 cut(s) 746, 1537, 1737, 2481, 3923
BseJI GATNNNNATC 2 cut(s) 1259, 4449
BseLI CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 3349, 3460, 3640, 3691, 4472
BseMI GCAATG 1 cut(s) 103
BseRI GAGGAG 7 cut(s) 411, 414, 499, 502, 2115, 2118, 2720
BseSI GKGCMC 1 cut(s) 1663
BseXI GCAGC 9 cut(s) 182, 281, 309, 941, 1331, 1575, 2192, 2193, 4403
BsgI GTGCAG 1 cut(s) 528
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 5 cut(s) 29, 485, 1290, 2810, 4510
BshNI GGYRCC 1 cut(s) 4039
BshTI ACCGGT 1 cut(s) 1633
BsiEI CGRYCG 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 2 cut(s) 1425, 3439
BsiSI CCGG 2 cut(s) 1426, 1634
BslFI GGGAC 7 cut(s) 183, 661, 727, 1018, 1401, 2845, 3735
BslI CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 3349, 3460, 3640, 3691, 4472
BsmAI GTCTC 7 cut(s) 245, 2698, 3440, 3851, 3912, 4070, 4609
BsmFI GGGAC 7 cut(s) 183, 661, 727, 1018, 1401, 2845, 3735
BsmI GAATGC 2 cut(s) 2010, 4156
BsnI GGCC 5 cut(s) 29, 485, 1290, 2810, 4510
Bso31I GGTCTC 4 cut(s) 2698, 3440, 3912, 4070
BsoBI CYCGRG 2 cut(s) 1425, 3439
Bsp1286I GDGCHC 3 cut(s) 213, 1663, 2564
Bsp1407I TGTACA 2 cut(s) 2456, 2517
Bsp19I CCATGG 1 cut(s) 3708
BspACI CCGC 5 cut(s) 38, 428, 2651, 3035, 3867
BspANI GGCC 5 cut(s) 29, 485, 1290, 2810, 4510
BspHI TCATGA 3 cut(s) 687, 2361, 3010
BspLI GGNNCC 5 cut(s) 366, 750, 1431, 4041, 4570
BspMAI CTGCAG 1 cut(s) 299
BspMI ACCTGC 6 cut(s) 550, 1597, 3300, 3597, 3642, 4314
BspOI GCTAGC 2 cut(s) 507, 1722
BspQI GCTCTTC 1 cut(s) 3657
BspT107I GGYRCC 1 cut(s) 4039
BspTI CTTAAG 2 cut(s) 3200, 3542
BspTNI GGTCTC 4 cut(s) 2698, 3440, 3912, 4070
BsrDI GCAATG 1 cut(s) 103
BsrFI RCCGGY 1 cut(s) 1633
BsrGI TGTACA 2 cut(s) 2456, 2517
BssAI RCCGGY 1 cut(s) 1633
BssNI GRCGYC 1 cut(s) 2391
BssSI CACGAG 2 cut(s) 933, 4512
BssT1I CCWWGG 8 cut(s) 368, 1167, 1264, 2746, 3366, 3708, 3894, 4583
Bst2BI CACGAG 2 cut(s) 933, 4512
Bst2UI CCWGG 5 cut(s) 746, 1537, 1737, 2481, 3923
Bst6I CTCTTC 7 cut(s) 170, 265, 714, 1839, 2060, 3657, 4104
BstACI GRCGYC 1 cut(s) 2391
BstAFI CTTAAG 2 cut(s) 3200, 3542
BstAPI GCANNNNNTGC 1 cut(s) 3362
BstAUI TGTACA 2 cut(s) 2456, 2517
BstBAI YACGTR 2 cut(s) 842, 1933
BstC8I GCNNGC 6 cut(s) 505, 1720, 2211, 3025, 3604, 4427
BstDSI CCRYGG 1 cut(s) 3708
BstEII GGTNACC 2 cut(s) 3287, 3629
BstENI CCTNNNNNAGG 1 cut(s) 343
BstMAI GTCTC 7 cut(s) 245, 2698, 3440, 3851, 3912, 4070, 4609
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 7 cut(s) 35, 636, 3362, 3826, 3939, 4388, 4435
BstNI CCWGG 5 cut(s) 746, 1537, 1737, 2481, 3923
BstNSI RCATGY 4 cut(s) 258, 294, 2629, 4429
BstPI GGTNACC 2 cut(s) 3287, 3629
BstSCI CCNGG 7 cut(s) 744, 1424, 1425, 1535, 1735, 2479, 3921
BstSFI CTRYAG 2 cut(s) 295, 1596
BstSLI GKGCMC 1 cut(s) 1663
BstSNI TACGTA 1 cut(s) 1933
BstV1I GCAGC 9 cut(s) 182, 281, 309, 941, 1331, 1575, 2192, 2193, 4403
BstV2I GAAGAC 1 cut(s) 2979
BstXI CCANNNNNNTGG 2 cut(s) 1860, 2818
Bsu36I CCTNAGG 1 cut(s) 514
BsuI GTATCC 2 cut(s) 1342, 3031
BsuRI GGCC 5 cut(s) 29, 485, 1290, 2810, 4510
BtgI CCRYGG 1 cut(s) 3708
BtgZI GCGATG 2 cut(s) 743, 1998
BtrI CACGTC 1 cut(s) 161
BtsIMutI CAGTG 8 cut(s) 471, 479, 1947, 2138, 3638, 3789, 4005, 4103
BveI ACCTGC 6 cut(s) 550, 1597, 3300, 3597, 3642, 4314
Cac8I GCNNGC 6 cut(s) 505, 1720, 2211, 3025, 3604, 4427
CaiI CAGNNNCTG 2 cut(s) 1612, 4636
CciI TCATGA 3 cut(s) 687, 2361, 3010
Cfr10I RCCGGY 1 cut(s) 1633
Cfr13I GGNCC 4 cut(s) 328, 749, 1360, 2808
Cfr9I CCCGGG 1 cut(s) 1425
CseI GACGC 4 cut(s) 554, 1580, 3211, 3553
CsiI ACCWGGT 1 cut(s) 2479
Csp6I GTAC 9 cut(s) 43, 152, 905, 1756, 2157, 2457, 2518, 3807, 4040
CspAI ACCGGT 1 cut(s) 1633
CviQI GTAC 9 cut(s) 43, 152, 905, 1756, 2157, 2457, 2518, 3807, 4040
DraI TTTAAA 2 cut(s) 1480, 1897
DraIII CACNNNGTG 1 cut(s) 2483
DriI GACNNNNNGTC 1 cut(s) 3728
EaeI YGGCCR 3 cut(s) 27, 483, 4508
Eam1104I CTCTTC 7 cut(s) 170, 265, 714, 1839, 2060, 3657, 4104
Eam1105I GACNNNNNGTC 1 cut(s) 3728
EarI CTCTTC 7 cut(s) 170, 265, 714, 1839, 2060, 3657, 4104
Ecl136II GAGCTC 1 cut(s) 211
Eco105I TACGTA 1 cut(s) 1933
Eco130I CCWWGG 8 cut(s) 368, 1167, 1264, 2746, 3366, 3708, 3894, 4583
Eco147I AGGCCT 1 cut(s) 1290
Eco24I GRGCYC 2 cut(s) 213, 2564
Eco31I GGTCTC 4 cut(s) 2698, 3440, 3912, 4070
Eco32I GATATC 4 cut(s) 895, 943, 1474, 4225
Eco47I GGWCC 3 cut(s) 328, 749, 1360
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 5 cut(s) 344, 452, 2309, 3293, 3635
Eco81I CCTNAGG 1 cut(s) 514
Eco88I CYCGRG 2 cut(s) 1425, 3439
Eco91I GGTNACC 2 cut(s) 3287, 3629
EcoICRI GAGCTC 1 cut(s) 211
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 1360
EcoO65I GGTNACC 2 cut(s) 3287, 3629
EcoRI GAATTC 1 cut(s) 1413
EcoRII CCWGG 5 cut(s) 744, 1535, 1735, 2479, 3921
EcoRV GATATC 4 cut(s) 895, 943, 1474, 4225
EcoT14I CCWWGG 8 cut(s) 368, 1167, 1264, 2746, 3366, 3708, 3894, 4583
EcoT22I ATGCAT 1 cut(s) 2010
EcoT38I GRGCYC 2 cut(s) 213, 2564
ErhI CCWWGG 8 cut(s) 368, 1167, 1264, 2746, 3366, 3708, 3894, 4583
FalI AAGNNNNNCTT 8 cut(s) 167, 199, 584, 616, 3313, 3345, 3887, 3919
FaqI GGGAC 7 cut(s) 183, 661, 727, 1018, 1401, 2845, 3735
FbaI TGATCA 3 cut(s) 1254, 2686, 4201
FblI GTMKAC 3 cut(s) 228, 1786, 3778
Fnu4HI GCNGC 9 cut(s) 171, 295, 298, 955, 1320, 1589, 2181, 2207, 4392
FriOI GRGCYC 2 cut(s) 213, 2564
Fsp4HI GCNGC 9 cut(s) 171, 295, 298, 955, 1320, 1589, 2181, 2207, 4392
GluI GCNGC 9 cut(s) 171, 295, 298, 955, 1320, 1589, 2181, 2207, 4392
GsuI CTGGAG 5 cut(s) 1558, 1938, 2222, 2845, 3662
HaeIII GGCC 5 cut(s) 29, 485, 1290, 2810, 4510
HapII CCGG 2 cut(s) 1426, 1634
HgaI GACGC 4 cut(s) 554, 1580, 3211, 3553
Hin1I GRCGYC 1 cut(s) 2391
HincII GTYRAC 1 cut(s) 2623
HindII GTYRAC 1 cut(s) 2623
HindIII AAGCTT 4 cut(s) 2075, 2930, 3074, 4477
HpaII CCGG 2 cut(s) 1426, 1634
Hpy99I CGWCG 2 cut(s) 162, 2393
HpyCH4IV ACGT 6 cut(s) 160, 841, 1932, 2025, 2391, 4348
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 636, 3362, 3826, 3939, 4388, 4435
HpySE526I ACGT 6 cut(s) 160, 841, 1932, 2025, 2391, 4348
Hsp92I GRCGYC 1 cut(s) 2391
KpnI GGTACC 1 cut(s) 4043
Ksp22I TGATCA 3 cut(s) 1254, 2686, 4201
LguI GCTCTTC 1 cut(s) 3657
LmnI GCTCC 9 cut(s) 58, 305, 512, 1919, 2203, 3257, 3599, 4434, 4633
Lsp1109I GCAGC 9 cut(s) 182, 281, 309, 941, 1331, 1575, 2192, 2193, 4403
LweI GCATC 7 cut(s) 157, 866, 1090, 1522, 2593, 4200, 4587
MabI ACCWGGT 1 cut(s) 2479
MaeII ACGT 6 cut(s) 160, 841, 1932, 2025, 2391, 4348
MfeI CAATTG 6 cut(s) 921, 1224, 1854, 2578, 2591, 2895
MhlI GDGCHC 3 cut(s) 213, 1663, 2564
MlsI TGGCCA 3 cut(s) 29, 485, 4510
MluNI TGGCCA 3 cut(s) 29, 485, 4510
MlyI GAGTC 6 cut(s) 1000, 3155, 3190, 3497, 3532, 3653
MmeI TCCRAC 6 cut(s) 198, 1146, 1633, 2812, 3402, 4105
Mox20I TGGCCA 3 cut(s) 29, 485, 4510
Mph1103I ATGCAT 1 cut(s) 2010
MroXI GAANNNNTTC 4 cut(s) 1677, 1848, 2636, 4480
MscI TGGCCA 3 cut(s) 29, 485, 4510
MslI CAYNNNNRTG 3 cut(s) 1957, 2312, 3794
Msp20I TGGCCA 3 cut(s) 29, 485, 4510
MspA1I CMGCKG 2 cut(s) 430, 3869
MspCI CTTAAG 2 cut(s) 3200, 3542
MspI CCGG 2 cut(s) 1426, 1634
MspR9I CCNGG 7 cut(s) 746, 1426, 1427, 1537, 1737, 2481, 3923
MunI CAATTG 6 cut(s) 921, 1224, 1854, 2578, 2591, 2895
Mva1269I GAATGC 2 cut(s) 2010, 4156
MvaI CCWGG 5 cut(s) 746, 1537, 1737, 2481, 3923
MwoI GCNNNNNNNGC 7 cut(s) 35, 636, 3362, 3826, 3939, 4388, 4435
NciI CCSGG 2 cut(s) 1426, 1427
NcoI CCATGG 1 cut(s) 3708
NheI GCTAGC 2 cut(s) 503, 1718
NlaIV GGNNCC 5 cut(s) 366, 750, 1431, 4041, 4570
NmuCI GTSAC 9 cut(s) 1105, 2021, 2355, 2384, 3147, 3287, 3489, 3629, 4019
NsiI ATGCAT 1 cut(s) 2010
NspI RCATGY 4 cut(s) 258, 294, 2629, 4429
PaeI GCATGC 1 cut(s) 4429
PagI TCATGA 3 cut(s) 687, 2361, 3010
PaqCI CACCTGC 1 cut(s) 550
PasI CCCWGGG 1 cut(s) 745
PceI AGGCCT 1 cut(s) 1290
PciI ACATGT 2 cut(s) 254, 2625
PciSI GCTCTTC 1 cut(s) 3657
PcsI WCGNNNNNNNCGW 1 cut(s) 2385
PctI GAATGC 2 cut(s) 2010, 4156
PdmI GAANNNNTTC 4 cut(s) 1677, 1848, 2636, 4480
PflFI GACNNNGTC 1 cut(s) 2390
PflMI CCANNNNNTGG 4 cut(s) 309, 3349, 3460, 3691
PfoI TCCNGGA 1 cut(s) 1735
PinAI ACCGGT 1 cut(s) 1633
PkrI GCNGC 9 cut(s) 172, 296, 299, 956, 1321, 1590, 2182, 2208, 4393
PleI GAGTC 6 cut(s) 1000, 3154, 3190, 3496, 3532, 3652
PpsI GAGTC 6 cut(s) 1000, 3154, 3190, 3496, 3532, 3652
Ppu21I YACGTR 2 cut(s) 842, 1933
PpuMI RGGWCCY 1 cut(s) 1360
PscI ACATGT 2 cut(s) 254, 2625
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 1360
Psp6I CCWGG 5 cut(s) 744, 1535, 1735, 2479, 3921
PspEI GGTNACC 2 cut(s) 3287, 3629
PspGI CCWGG 5 cut(s) 744, 1535, 1735, 2479, 3921
PspN4I GGNNCC 5 cut(s) 366, 750, 1431, 4041, 4570
PspPI GGNCC 4 cut(s) 328, 749, 1360, 2808
PspPPI RGGWCCY 1 cut(s) 1360
PstI CTGCAG 1 cut(s) 299
PstNI CAGNNNCTG 2 cut(s) 1612, 4636
PsyI GACNNNGTC 1 cut(s) 2390
RsaI GTAC 9 cut(s) 44, 153, 906, 1757, 2158, 2458, 2519, 3808, 4041
RsaNI GTAC 9 cut(s) 43, 152, 905, 1756, 2157, 2457, 2518, 3807, 4040
RseI CAYNNNNRTG 3 cut(s) 1957, 2312, 3794
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 3657
SatI GCNGC 9 cut(s) 171, 295, 298, 955, 1320, 1589, 2181, 2207, 4392
Sau96I GGNCC 4 cut(s) 328, 749, 1360, 2808
ScaI AGTACT 2 cut(s) 2158, 3808
SchI GAGTC 6 cut(s) 1000, 3155, 3190, 3497, 3532, 3653
ScrFI CCNGG 7 cut(s) 746, 1426, 1427, 1537, 1737, 2481, 3923
SduI GDGCHC 3 cut(s) 213, 1663, 2564
SexAI ACCWGGT 1 cut(s) 2479
SfaNI GCATC 7 cut(s) 157, 866, 1090, 1522, 2593, 4200, 4587
SfcI CTRYAG 2 cut(s) 295, 1596
SinI GGWCC 3 cut(s) 328, 749, 1360
SmaI CCCGGG 1 cut(s) 1427
SmiMI CAYNNNNRTG 3 cut(s) 1957, 2312, 3794
SnaBI TACGTA 1 cut(s) 1933
SphI GCATGC 1 cut(s) 4429
SseBI AGGCCT 1 cut(s) 1290
SsiI CCGC 5 cut(s) 38, 428, 2651, 3035, 3867
SspI AATATT 3 cut(s) 1371, 2332, 4189
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 1290
StyD4I CCNGG 7 cut(s) 744, 1424, 1425, 1535, 1735, 2479, 3921
StyI CCWWGG 8 cut(s) 368, 1167, 1264, 2746, 3366, 3708, 3894, 4583
TaiI ACGT 6 cut(s) 163, 844, 1935, 2028, 2394, 4351
TaqI TCGA 8 cut(s) 2223, 2379, 2492, 2739, 4011, 4319, 4377, 4527
TaqII GACCGA 1 cut(s) 1329
TatI WGTACW 5 cut(s) 42, 2156, 2456, 2517, 3806
TscAI CASTG 8 cut(s) 478, 486, 1947, 2145, 3645, 3796, 4012, 4110
TseFI GTSAC 9 cut(s) 1105, 2021, 2355, 2384, 3147, 3287, 3489, 3629, 4019
TseI GCWGC 9 cut(s) 170, 294, 297, 954, 1319, 1588, 2180, 2206, 4391
Tsp45I GTSAC 9 cut(s) 1105, 2021, 2355, 2384, 3147, 3287, 3489, 3629, 4019
TspGWI ACGGA 4 cut(s) 320, 1477, 1773, 2246
TspMI CCCGGG 1 cut(s) 1425
TspRI CASTG 8 cut(s) 478, 486, 1947, 2145, 3645, 3796, 4012, 4110
Tth111I GACNNNGTC 1 cut(s) 2390
Van91I CCANNNNNTGG 4 cut(s) 309, 3349, 3460, 3691
Vha464I CTTAAG 2 cut(s) 3200, 3542
VpaK11BI GGWCC 3 cut(s) 328, 749, 1360
XagI CCTNNNNNAGG 1 cut(s) 343
XapI RAATTY 8 cut(s) 397, 927, 1413, 1677, 2419, 2636, 3094, 4261
XbaI TCTAGA 1 cut(s) 2506
XceI RCATGY 4 cut(s) 258, 294, 2629, 4429
XcmI CCANNNNNNNNNTGG 2 cut(s) 478, 4518
XmaI CCCGGG 1 cut(s) 1425
XmiI GTMKAC 3 cut(s) 228, 1786, 3778
XmnI GAANNNNTTC 4 cut(s) 1677, 1848, 2636, 4480
ZraI GACGTC 1 cut(s) 2392
ZrmI AGTACT 2 cut(s) 2158, 3808
Zsp2I ATGCAT 1 cut(s) 2010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.