Rh6CG260500

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000203
Physical Location & Seq
Reverse (-)
43290106 .. 43298343
8238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG260500.1

Sequence Viewer

Length: 4470 bp
ATGGCAGAAATATGTATTTCAGTTGTGGAAAAATTAGCAGAGTGCACAGTCTCACCTGTTCTGCGACAGTTTGGTTATCTGATTAACTACAAGAGCAATGTTGAAGATCTCACCGTGAAGGTTACAACTCTGACTTCCAAAAGAGATGGGGTACAACAACGTGTGGAAGCAGCAAACAGAAACTTGGAGGCTATTCTGCCCGAAGTTCAGAACTGGTTGGACCAAGTAAACCAGACCATACAAGAAAAGGAGGCGCGTTTTGAAAAAGGAAGATTATCGAAGGCAACGACATGCTGCAGCTCCAATGGGTGGTGTCGAAATCTAAAGGTCCGCCATTCCTTGAGTAGGAAAGCAACGAAGATGGCTCCAAAGGTTGATGTCCTCCTTACAGAAGGAGATTTCCCCATAATATCATGTCCTGCTCCTCCGCGGAAGATTGAATATCCAGCCATACAATATTTTGAGGATTCCACCGAGGCCCCGGCCAAGCCACATGAAGGGGCAAGCTCCTCCTTAGGAACATCTAATCCTGCTAAACCACCTGCACCTATGGATCTCTATGCGCGTCTGCAATATAGGTTGCCTTACACAAAGGATCTCTTGGCGGCTCTGCAAGATGACAAGATCAGTATGATTGGCATTTCTGGGACAATCAGAGAGATAGATACAGTAACAACTAAAGAGTTCATGAAAAGAATGAAACAAAAAAAGCTCTTTGAAGAGGTTGCCATGGCAGTTGTGTCCCAGGATCCAGACTTGAAACGAATACAAGGTGAAATTGCAGAAATGCTAGACCTGAAATTGGAGATTGGGAATTTGGTTGAACGGGCAGATAGGCTACGTGCTGTATTATTGGCATCCGATTCTAAGAGGATTCTTGTAATACTGACTGATGTCTGTGGAATACTTGATTTGGATGCAATTGGAATTTCTCATGTTGATATCAAGAAAAGCTGCAAGATCATGTTGATTTCACAACTATCAAACGTATTTGGTGAAATGGGGACTCAGAGCAATTTCAAATTGTTATTACAATCCACTCCCATAGCAAGACCCGTTGAGTTTGAATCAAGAATTTCAATCATCAGAGATGTAATGGATGCTCTGACAGACGATGAATCCAACCCCATTGTAATCTGTGGCATGGGGGGAATTGGCAAAACAACCTTGGTGATGGAAATTGGTGCAAAAGCGAGTGAAGTAGATCTATTTGATGATGTTGCTATTGCAGAATTTACTCAAGAACCAGACTTGATCAAAATCCAAGGTAAAATTGCTAATGCTCTAGGTCTGGAACTTAAAGCAGAGGATGATAGAGCAGCCAAGTTACGAGAAAGATTATCAATGGGTATCAAAAAGGTCCTTATAATATTGGACAATGTTTGGACACAACTAAATTTGTGGGAAATAGGGATTCCAATTTCCCGTGATCCAAAATGTTGTAAACTTCTAGTATCATCACGGAACCAAGATATCTTCAAAGAGATGAAAACCAAAAGAAATTTCCTCATTGCTGGTTTGTCAAAACATGATGCTTGGAGTTTGTTTAAGAAGGTAGCAGGAGGGTCCATAGAATTTGATCTCGAGCTGCGTCCTATAGCAAAACAGGTTCTGGATGAATGTGATGGCTTACCAGTTGCAATTTCTACAGTTGGAGGGGCACTACAAGGTGAAAGCATTCCAAAATGGAGAAATGCACTTAGACAACTAAGACAGGCTTGCCCAGAAGACGTTCCAGGAGTCATAGAGCATGTGTACGGAAAAATAGCGTTCAGCTTTGAATGTCTACCAAGCGAGCAAGCCAAATCATGTTTTTTGCTATGTTGTGCATTTCCAGAAAGTGAAGAGTTTCCAATTGAATGGTTGGTTATGTTTGGGATTGGGCTTGGACTTTTTAAAGACATTGATTCAATATCTGAAGCAAGGGACTACTTAACAACATTGGTTGACACACTCAAAAATCGTTCTTTGTTGTTAGAGGACGATGAGAAAGATTATGTTAGAATGCATGATGTGGTGCGTGATGTAGCCGTGTATATAGCCTCCAAAGAGTTGGCACAAAAGAAGAGTGATAAGCTTGAAAGGAGATTTATGTGTAGAGAAGCTGTGGAATTGAACCATTTGCTCAAGAGTAGTTCACTGACAACCGGTATGAGTACTATTGAGCTTTTAGTGTTGGTACCCACAGATGGAATTGAACTGGAGCTGCCGGCCACAATTTTCGATGGAATGGAAAATCTCAAGGTTTTAGAGATCTGGAGATCAATCCTCCCATCCCTTTCACTTCTGAAGAATCTGCAAACGCTCATTCTAGTGGGTTGCCGTCTCAATATTGATGTCATTGGAGAGCTACGGTCACTCATGATACTCCATTTACAGGAATCTGACGTCAAACAGTTGCCTGATGCATTCAAAAATTTATCTAATCTGAGGTCGTTAAATTTGACAGGATGTACAAAACTTAAAGTAATCTCACCAGGCGTTCTGTCGAGTTTATCTCATCTAGAAGAATTGTACATGTGGGATAGCTTTGAAGATTGGGTGGTTGAGAAACTGGGCTCAACAGAAACAATCAATTGGATCTCTAAATTGGAGGTTAATGCCCAACAAGAAGAAGTAGACATATGGAAGAAATTGGGCTCAACAAAAACAATCTATTGGATCTCTCAATGGGAGGTTGATGCCCAACCAGAAAAAGTCGACATATGGAAGAAACTCAACAGGTTGGCAGTTGAGAAACTAGCCTCAACAGAAACAACAACTGATCAGGAGGATGAGGATGAGGATGCAGATAGTAGCGAATTAGAGGAACTCGACAACCTTGGAGATCTGTTTGCAGGGTATGTTCGAGATTTAGAAGGAGACTACGACGATCTCACAAGGTGGGCCAACTCTATGGACTGGAGAGTTGGATTACTTTCAGAGCTACTTTCCTTGTCCCGTTTGACTACTTTAGAAGTGGTTTTACCACCAATTGAGATTCTGCGAACCAGTAAGCTATTTCATAAGCTTGAAAGATTTAAGATCTCTATTGGATGGGATGAGCATGAAGACTTGGAGGACTTAGAGGGTAACTACTTGAGAGTTTATAGTCTTGATGGAAGCTCTCTTGCGGATACTGAAATCAGTTTGTTGTTGAGGAAAACTAACAAGCTTGAGTTAAAAATGAAGAATTTGAGAGACCCTCTCAATGTCTTAGATGTAAACTGCTGTGCAAACTTGGAGTCACTTGCCCTCAAAGACTGTGATTCTTTGGAATATCTGATTGACATGACTGTTAAACAAGATACTCCACGAAGCGTCTTTCCTGCATTGAATTATCTGGAAATCGATGGAGCAAGGAGGCTGAAAGAGATTTTTCATGGTGACCTGCCACTAGGGTCGCTTCAGAAACTAGAAACGTTAGATTTACTGAATCTACCTGCATTGACGACTATTTGGACTATTAGAAGCCAATCTAGATGGTTGGGAGATCTGGCAGTTGTCAATCGCATATCAATAAGCAATTGCCAGTCATTGGAAGCAATTTTTGCCTTGGAACGATTGGGACATCATGTCAAAGAGATCAACTTCCTTAGTTTGACAGGGTTGAAACTTGAAGGTCTACCAAGCTTCACTGGAATGAGCAAAAGTACTTGTAAGGGGATGGAAAAGCAACTGCAAGTCAGCAGTCTCTATCCCAAAAGTTTGACCGTTGAACATTTACTTTTCTCTGACCCTAAGGTTGTCTTTCCCGTCTTGAGAGATCTGAACATTCAAGGCCTAGGCAACTTGAAAGAGATATGGAATAACCAACTTTCACCCAACTCCTTCTCTGAATTAAGAACGCTCACTGTCGAGGATTGTCACAGACTATTGCATTTGGGACCAACACAAATGCAAAATAGATTGCAGAGATTGGAGACCATACGGGTAAAGAACTGTTCTTCACTGGAAGAGATTTTTGAAGTCGGAAGATTGACCATCAATGAAGGGAATGCTTCAATTTCTCAGTCAGACAAAATATCTTCAAATATTTCCCAACCAGATCAAGGGATGCAAATAAATGATATCATGGATTTTGAACAATCTTGTCAAGGCTTTCAAAATTTAACTAAACTAGATGTCAGTTGGTGTCGCAGTTTGAGATACCTGCTCACTCCTTCGATTGCCAGAGGTCTTGTCAAGCTCAAAAGTCTAGAGATAAGATTTTGCAAGAAGATCGAAACAATAGTCGCAGCAGATGAGGGTGAAGAAACAGAGGATGAAAGCATGCTCCCTCAGCTGTCTTCTTTGATTCTTTTGGATCTACCAAATCTTGGAAGCTTTTCGCAAGGCAAATATACTTTTGATTGGCCACTAGTGGAAAAGATCATCATTTACGATTGCATCAATATGAACAACTTTTGTTCAGGATCCCTCAAGATCCCATGGGAAGTGAGCATAGATGTATCTAATTCTGGTGAGAATCTTGAGCAGGAGTTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1489

Amino Acids

167.6

Weight (kDa)

5.02

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 209 - 338 7.3e-09 NB-ARC domain
NB-ARC PF00931 362 - 520 4.1e-23 NB-ARC domain
LRR_14 PF23598 780 - 861 3.6e-06 Leucine-rich repeat region
LRR_8 PF13855 785 - 840 2.6e-07 Leucine rich repeat
LRR_RPS2 PF23247 1052 - 1178 5.9e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1256 - 1399 3.6e-26 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1359 - 1461 2.9e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1367
AarI CACCTGC 1 cut(s) 550
AatII GACGTC 1 cut(s) 2391
Acc36I ACCTGC 4 cut(s) 550, 3378, 3430, 4140
Acc65I GGTACC 1 cut(s) 2179
AccB1I GGYRCC 1 cut(s) 2179
AccB7I CCANNNNNTGG 3 cut(s) 309, 3517, 4298
AccI GTMKAC 4 cut(s) 1786, 2619, 2700, 3604
AccII CGCG 3 cut(s) 256, 430, 565
AciI CCGC 5 cut(s) 331, 428, 430, 605, 3113
AclI AACGTT 1 cut(s) 3401
AcoI YGGCCR 3 cut(s) 483, 2211, 4334
AcuI CTGAAG 3 cut(s) 1938, 2309, 3371
AcyI GRCGYC 1 cut(s) 2388
AdeI CACNNNGTG 1 cut(s) 2883
AfaI GTAC 7 cut(s) 153, 1757, 2158, 2181, 2455, 2516, 3634
AfiI CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 2189, 2377, 3517, 4031, 4298
AflIII ACRYGT 2 cut(s) 160, 2517
AgeI ACCGGT 1 cut(s) 2147
AhdI GACNNNNNGTC 1 cut(s) 3554
AhlI ACTAGT 1 cut(s) 4339
AjnI CCWGG 3 cut(s) 744, 1735, 2476
AjuI GAANNNNNNNTTGG 2 cut(s) 1487, 1519
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 6 cut(s) 55, 2330, 2856, 3174, 3677, 3896
Alw44I GTGCAC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 1612
Ama87I CYCGRG 1 cut(s) 1583
AoxI GGCC 6 cut(s) 477, 483, 2211, 2886, 3760, 4334
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 8 cut(s) 170, 294, 297, 954, 1319, 1588, 2206, 4217
ArsI GACNNNNNNTTYG 4 cut(s) 3209, 3241, 4197, 4229
AsiGI ACCGGT 1 cut(s) 2147
Asp700I GAANNNNTTC 4 cut(s) 1677, 1731, 1848, 4306
Asp718I GGTACC 1 cut(s) 2179
AspA2I CCTAGG 1 cut(s) 3763
AspLEI GCGC 2 cut(s) 256, 565
AspS9I GGNCC 7 cut(s) 220, 328, 478, 1360, 1566, 2886, 3866
AsuC2I CCSGG 1 cut(s) 482
AvaI CYCGRG 1 cut(s) 1583
AvaII GGWCC 5 cut(s) 220, 328, 1360, 1566, 3866
AvrII CCTAGG 1 cut(s) 3763
AxyI CCTNAGG 2 cut(s) 514, 3720
BaeGI GKGCMC 2 cut(s) 47, 1663
BalI TGGCCA 1 cut(s) 4336
BamHI GGATCC 2 cut(s) 748, 4394
BanI GGYRCC 1 cut(s) 2179
BanII GRGCYC 2 cut(s) 2561, 2642
BarI GAAGNNNNNNTAC 2 cut(s) 2949, 2981
BbsI GAAGAC 3 cut(s) 1734, 3057, 4260
Bbv12I GWGCWC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 4260
BbvI GCAGC 8 cut(s) 182, 281, 309, 941, 1331, 1575, 2193, 4229
BceAI ACGGC 2 cut(s) 2015, 2307
BcgI CGANNNNNNTGC 2 cut(s) 4183, 4217
BciT130I CCWGG 3 cut(s) 746, 1737, 2478
BciVI GTATCC 1 cut(s) 3109
BclI TGATCA 2 cut(s) 1254, 2764
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 6 cut(s) 55, 2330, 2856, 3174, 3677, 3896
BcuI ACTAGT 1 cut(s) 4339
BfmI CTRYAG 3 cut(s) 295, 1596, 1647
BfuAI ACCTGC 4 cut(s) 550, 3378, 3430, 4140
BfuI GTATCC 1 cut(s) 3109
BglII AGATCT 7 cut(s) 106, 1204, 2253, 2827, 3024, 3472, 3745
BisI GCNGC 9 cut(s) 171, 295, 298, 606, 955, 1320, 1589, 2207, 4218
BlnI CCTAGG 1 cut(s) 3763
BlsI GCNGC 9 cut(s) 172, 296, 299, 607, 956, 1321, 1590, 2208, 4219
BmcAI AGTACT 2 cut(s) 2158, 3634
Bme1390I CCNGG 4 cut(s) 482, 746, 1737, 2478
Bme18I GGWCC 5 cut(s) 220, 328, 1360, 1566, 3866
BmeRI GACNNNNNGTC 1 cut(s) 3554
BmeT110I CYCGRG 1 cut(s) 1583
BmgT120I GGNCC 7 cut(s) 220, 328, 478, 1360, 1566, 2886, 3866
BmiI GGNNCC 8 cut(s) 366, 480, 750, 1466, 1567, 2181, 3867, 4396
BmrFI CCNGG 4 cut(s) 482, 746, 1737, 2478
BmrI ACTGGG 1 cut(s) 2564
BmsI GCATC 9 cut(s) 866, 907, 1090, 1522, 2395, 2671, 2776, 4026, 4377
BmuI ACTGGG 1 cut(s) 2564
BoxI GACNNNNGTC 1 cut(s) 893
BpiI GAAGAC 3 cut(s) 1734, 3057, 4260
BplI GAGNNNNNCTC 6 cut(s) 2482, 2514, 3169, 3201, 3171, 3203
BpmI CTGGAG 3 cut(s) 2222, 2278, 2923
Bpu10I CCTNAGC 1 cut(s) 4260
BpuEI CTTGAG 8 cut(s) 361, 1224, 2111, 2225, 3100, 3176, 3760, 4385
BpuMI CCSGG 1 cut(s) 482
Bsa29I ATCGAT 1 cut(s) 3330
BsaAI YACGTR 1 cut(s) 842
BsaBI GATNNNNATC 1 cut(s) 1259
BsaHI GRCGYC 1 cut(s) 2388
BsaI GGTCTC 2 cut(s) 3174, 3896
BsaWI WCCGGW 1 cut(s) 2147
Bsc4I CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 2189, 2377, 3517, 4031, 4298
Bse118I RCCGGY 2 cut(s) 2147, 2209
Bse1I ACTGG 9 cut(s) 218, 1634, 2205, 2559, 2906, 2991, 3511, 3622, 3936
Bse21I CCTNAGG 2 cut(s) 514, 3720
Bse3DI GCAATG 2 cut(s) 103, 1509
Bse8I GATNNNNATC 1 cut(s) 1259
BseBI CCWGG 3 cut(s) 746, 1737, 2478
BseCI ATCGAT 1 cut(s) 3330
BseJI GATNNNNATC 1 cut(s) 1259
BseLI CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 2189, 2377, 3517, 4031, 4298
BseMI GCAATG 2 cut(s) 103, 1509
BseMII CTCAG 4 cut(s) 1022, 2420, 4004, 4274
BseNI ACTGG 9 cut(s) 218, 1634, 2205, 2559, 2906, 2991, 3511, 3622, 3936
BseRI GAGGAG 2 cut(s) 414, 499
BseSI GKGCMC 2 cut(s) 47, 1663
BseXI GCAGC 8 cut(s) 182, 281, 309, 941, 1331, 1575, 2193, 4229
BsgI GTGCAG 1 cut(s) 528
Bsh1236I CGCG 3 cut(s) 256, 430, 565
BshFI GGCC 6 cut(s) 479, 485, 2213, 2888, 3762, 4336
BshNI GGYRCC 1 cut(s) 2179
BshTI ACCGGT 1 cut(s) 2147
BshVI ATCGAT 1 cut(s) 3330
BsiHKAI GWGCWC 1 cut(s) 47
BsiHKCI CYCGRG 1 cut(s) 1583
BsiSI CCGG 3 cut(s) 482, 2148, 2210
BslFI GGGAC 7 cut(s) 661, 727, 1018, 1940, 2923, 3561, 3879
BslI CCNNNNNNNGG 9 cut(s) 309, 345, 497, 802, 2189, 2377, 3517, 4031, 4298
BsmAI GTCTC 6 cut(s) 55, 2330, 2856, 3174, 3677, 3896
BsmBI CGTCTC 1 cut(s) 2330
BsmFI GGGAC 7 cut(s) 661, 727, 1018, 1940, 2923, 3561, 3879
BsmI GAATGC 4 cut(s) 1677, 2010, 2408, 3982
BsnI GGCC 6 cut(s) 479, 485, 2213, 2888, 3762, 4336
Bso31I GGTCTC 2 cut(s) 3174, 3896
BsoBI CYCGRG 1 cut(s) 1583
Bsp1286I GDGCHC 4 cut(s) 47, 1663, 2561, 2642
Bsp1407I TGTACA 2 cut(s) 2453, 2514
Bsp19I CCATGG 2 cut(s) 729, 4409
BspACI CCGC 5 cut(s) 331, 428, 430, 605, 3113
BspANI GGCC 6 cut(s) 479, 485, 2213, 2888, 3762, 4336
BspCNI CTCAG 4 cut(s) 1021, 2421, 4003, 4273
BspDI ATCGAT 1 cut(s) 3330
BspFNI CGCG 3 cut(s) 256, 430, 565
BspHI TCATGA 2 cut(s) 687, 2361
BspLI GGNNCC 8 cut(s) 366, 480, 750, 1466, 1567, 2181, 3867, 4396
BspMAI CTGCAG 1 cut(s) 299
BspMI ACCTGC 4 cut(s) 550, 3378, 3430, 4140
BspT107I GGYRCC 1 cut(s) 2179
BspTNI GGTCTC 2 cut(s) 3174, 3896
BsrDI GCAATG 2 cut(s) 103, 1509
BsrFI RCCGGY 2 cut(s) 2147, 2209
BsrGI TGTACA 2 cut(s) 2453, 2514
BsrI ACTGG 9 cut(s) 218, 1634, 2205, 2559, 2906, 2991, 3511, 3622, 3936
BssAI RCCGGY 2 cut(s) 2147, 2209
BssNI GRCGYC 1 cut(s) 2388
BssT1I CCWWGG 7 cut(s) 729, 1167, 1264, 2821, 3534, 3763, 4409
Bst2UI CCWGG 3 cut(s) 746, 1737, 2478
Bst6I CTCTTC 4 cut(s) 714, 1839, 2060, 3930
BstACI GRCGYC 1 cut(s) 2388
BstAPI GCANNNNNTGC 1 cut(s) 3530
BstAUI TGTACA 2 cut(s) 2453, 2514
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 6 cut(s) 505, 1720, 1796, 1800, 2211, 4253
BstDSI CCRYGG 3 cut(s) 428, 729, 4409
BstEII GGTNACC 1 cut(s) 3365
BstENI CCTNNNNNAGG 1 cut(s) 343
BstFNI CGCG 3 cut(s) 256, 430, 565
BstHHI GCGC 2 cut(s) 256, 565
BstMAI GTCTC 6 cut(s) 55, 2330, 2856, 3174, 3677, 3896
BstMWI GCNNNNNNNGC 2 cut(s) 3530, 4261
BstNI CCWGG 3 cut(s) 746, 1737, 2478
BstNSI RCATGY 4 cut(s) 294, 1754, 2521, 4255
BstPAI GACNNNNGTC 1 cut(s) 893
BstPI GGTNACC 1 cut(s) 3365
BstSCI CCNGG 4 cut(s) 480, 744, 1735, 2476
BstSFI CTRYAG 3 cut(s) 295, 1596, 1647
BstSLI GKGCMC 2 cut(s) 47, 1663
BstUI CGCG 3 cut(s) 256, 430, 565
BstV1I GCAGC 8 cut(s) 182, 281, 309, 941, 1331, 1575, 2193, 4229
BstV2I GAAGAC 3 cut(s) 1734, 3057, 4260
BstXI CCANNNNNNTGG 3 cut(s) 1860, 2053, 2896
Bsu15I ATCGAT 1 cut(s) 3330
Bsu36I CCTNAGG 2 cut(s) 514, 3720
BsuI GTATCC 1 cut(s) 3109
BsuRI GGCC 6 cut(s) 479, 485, 2213, 2888, 3762, 4336
BsuTUI ATCGAT 1 cut(s) 3330
BtgI CCRYGG 3 cut(s) 428, 729, 4409
BtsIMutI CAGTG 4 cut(s) 2138, 3615, 3831, 3929
BveI ACCTGC 4 cut(s) 550, 3378, 3430, 4140
Cac8I GCNNGC 6 cut(s) 505, 1720, 1796, 1800, 2211, 4253
CaiI CAGNNNCTG 1 cut(s) 1612
CciI TCATGA 2 cut(s) 687, 2361
CfoI GCGC 2 cut(s) 256, 565
Cfr10I RCCGGY 2 cut(s) 2147, 2209
Cfr13I GGNCC 7 cut(s) 220, 328, 478, 1360, 1566, 2886, 3866
Cfr42I CCGCGG 1 cut(s) 431
ClaI ATCGAT 1 cut(s) 3330
CseI GACGC 3 cut(s) 554, 1580, 3289
Csp6I GTAC 7 cut(s) 152, 1756, 2157, 2180, 2454, 2515, 3633
CspAI ACCGGT 1 cut(s) 2147
CviQI GTAC 7 cut(s) 152, 1756, 2157, 2180, 2454, 2515, 3633
DraI TTTAAA 1 cut(s) 1897
DraIII CACNNNGTG 1 cut(s) 2883
DriI GACNNNNNGTC 1 cut(s) 3554
EaeI YGGCCR 3 cut(s) 483, 2211, 4334
Eam1104I CTCTTC 4 cut(s) 714, 1839, 2060, 3930
Eam1105I GACNNNNNGTC 1 cut(s) 3554
EarI CTCTTC 4 cut(s) 714, 1839, 2060, 3930
EciI GGCGGA 1 cut(s) 320
Eco130I CCWWGG 7 cut(s) 729, 1167, 1264, 2821, 3534, 3763, 4409
Eco147I AGGCCT 1 cut(s) 3762
Eco24I GRGCYC 2 cut(s) 2561, 2642
Eco31I GGTCTC 2 cut(s) 3174, 3896
Eco32I GATATC 3 cut(s) 943, 1474, 4051
Eco47I GGWCC 5 cut(s) 220, 328, 1360, 1566, 3866
Eco57I CTGAAG 3 cut(s) 1938, 2309, 3371
Eco81I CCTNAGG 2 cut(s) 514, 3720
Eco88I CYCGRG 1 cut(s) 1583
Eco91I GGTNACC 1 cut(s) 3365
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoO109I RGGNCCY 2 cut(s) 478, 1360
EcoO65I GGTNACC 1 cut(s) 3365
EcoRII CCWGG 3 cut(s) 744, 1735, 2476
EcoRV GATATC 3 cut(s) 943, 1474, 4051
EcoT14I CCWWGG 7 cut(s) 729, 1167, 1264, 2821, 3534, 3763, 4409
EcoT22I ATGCAT 2 cut(s) 2010, 2410
EcoT38I GRGCYC 2 cut(s) 2561, 2642
ErhI CCWWGG 7 cut(s) 729, 1167, 1264, 2821, 3534, 3763, 4409
Esp3I CGTCTC 1 cut(s) 2330
FalI AAGNNNNNCTT 6 cut(s) 584, 616, 1702, 1734, 3713, 3745
FaqI GGGAC 7 cut(s) 661, 727, 1018, 1940, 2923, 3561, 3879
FauNDI CATATG 2 cut(s) 2624, 2705
FbaI TGATCA 2 cut(s) 1254, 2764
FblI GTMKAC 4 cut(s) 1786, 2619, 2700, 3604
Fnu4HI GCNGC 9 cut(s) 171, 295, 298, 606, 955, 1320, 1589, 2207, 4218
FriOI GRGCYC 2 cut(s) 2561, 2642
Fsp4HI GCNGC 9 cut(s) 171, 295, 298, 606, 955, 1320, 1589, 2207, 4218
GlaI GCGC 2 cut(s) 255, 564
GluI GCNGC 9 cut(s) 171, 295, 298, 606, 955, 1320, 1589, 2207, 4218
GsuI CTGGAG 3 cut(s) 2222, 2278, 2923
HaeIII GGCC 6 cut(s) 479, 485, 2213, 2888, 3762, 4336
HapII CCGG 3 cut(s) 482, 2148, 2210
HgaI GACGC 3 cut(s) 554, 1580, 3289
HhaI GCGC 2 cut(s) 256, 565
Hin1I GRCGYC 1 cut(s) 2388
Hin6I GCGC 2 cut(s) 254, 563
HinP1I GCGC 2 cut(s) 254, 563
HincII GTYRAC 2 cut(s) 1948, 2701
HindII GTYRAC 2 cut(s) 1948, 2701
HindIII AAGCTT 5 cut(s) 2075, 3008, 3152, 3610, 4303
HpaII CCGG 3 cut(s) 482, 2148, 2210
Hpy99I CGWCG 1 cut(s) 2873
HpyCH4IV ACGT 6 cut(s) 160, 841, 987, 1731, 2388, 3401
HpyF10VI GCNNNNNNNGC 2 cut(s) 3530, 4261
HpySE526I ACGT 6 cut(s) 160, 841, 987, 1731, 2388, 3401
Hsp92I GRCGYC 1 cut(s) 2388
HspAI GCGC 2 cut(s) 254, 563
KpnI GGTACC 1 cut(s) 2183
KroI GCCGGC 1 cut(s) 2209
KroNI GCCGGC 1 cut(s) 2211
Ksp22I TGATCA 2 cut(s) 1254, 2764
KspI CCGCGG 1 cut(s) 431
LmnI GCTCC 7 cut(s) 305, 370, 427, 512, 2203, 3335, 4260
Lsp1109I GCAGC 8 cut(s) 182, 281, 309, 941, 1331, 1575, 2193, 4229
LweI GCATC 9 cut(s) 866, 907, 1090, 1522, 2395, 2671, 2776, 4026, 4377
MaeII ACGT 6 cut(s) 160, 841, 987, 1731, 2388, 3401
MaeIII GTNAC 8 cut(s) 121, 670, 1326, 2355, 3071, 3225, 3365, 3845
MfeI CAATTG 5 cut(s) 921, 1854, 2575, 2973, 3505
MhlI GDGCHC 4 cut(s) 47, 1663, 2561, 2642
MlsI TGGCCA 1 cut(s) 4336
MluNI TGGCCA 1 cut(s) 4336
MlyI GAGTC 3 cut(s) 1000, 1749, 3233
MmeI TCCRAC 5 cut(s) 198, 1146, 1633, 2890, 3931
Mox20I TGGCCA 1 cut(s) 4336
Mph1103I ATGCAT 2 cut(s) 2010, 2410
MroNI GCCGGC 1 cut(s) 2209
MroXI GAANNNNTTC 4 cut(s) 1677, 1731, 1848, 4306
MscI TGGCCA 1 cut(s) 4336
MslI CAYNNNNRTG 3 cut(s) 2312, 3620, 4373
Msp20I TGGCCA 1 cut(s) 4336
MspA1I CMGCKG 2 cut(s) 430, 4264
MspI CCGG 3 cut(s) 482, 2148, 2210
MspR9I CCNGG 4 cut(s) 482, 746, 1737, 2478
MunI CAATTG 5 cut(s) 921, 1854, 2575, 2973, 3505
Mva1269I GAATGC 4 cut(s) 1677, 2010, 2408, 3982
MvaI CCWGG 3 cut(s) 746, 1737, 2478
MvnI CGCG 3 cut(s) 256, 430, 565
MwoI GCNNNNNNNGC 2 cut(s) 3530, 4261
NaeI GCCGGC 1 cut(s) 2211
NciI CCSGG 1 cut(s) 482
NcoI CCATGG 2 cut(s) 729, 4409
NdeI CATATG 2 cut(s) 2624, 2705
NgoMIV GCCGGC 1 cut(s) 2209
NlaIV GGNNCC 8 cut(s) 366, 480, 750, 1466, 1567, 2181, 3867, 4396
NmuCI GTSAC 4 cut(s) 2355, 3225, 3365, 3845
NsiI ATGCAT 2 cut(s) 2010, 2410
NspI RCATGY 4 cut(s) 294, 1754, 2521, 4255
PaeI GCATGC 1 cut(s) 4255
PaeR7I CTCGAG 1 cut(s) 1583
PagI TCATGA 2 cut(s) 687, 2361
PaqCI CACCTGC 1 cut(s) 550
PceI AGGCCT 1 cut(s) 3762
PciI ACATGT 1 cut(s) 2517
PcsI WCGNNNNNNNCGW 1 cut(s) 284
PctI GAATGC 4 cut(s) 1677, 2010, 2408, 3982
PdiI GCCGGC 1 cut(s) 2211
PdmI GAANNNNTTC 4 cut(s) 1677, 1731, 1848, 4306
PflMI CCANNNNNTGG 3 cut(s) 309, 3517, 4298
PfoI TCCNGGA 1 cut(s) 1735
PinAI ACCGGT 1 cut(s) 2147
PkrI GCNGC 9 cut(s) 172, 296, 299, 607, 956, 1321, 1590, 2208, 4219
PleI GAGTC 3 cut(s) 1000, 1748, 3232
PpsI GAGTC 3 cut(s) 1000, 1748, 3232
Ppu21I YACGTR 1 cut(s) 842
PpuMI RGGWCCY 1 cut(s) 1360
PscI ACATGT 1 cut(s) 2517
PshAI GACNNNNGTC 1 cut(s) 893
PsiI TTATAA 1 cut(s) 1367
Psp1406I AACGTT 1 cut(s) 3401
Psp5II RGGWCCY 1 cut(s) 1360
Psp6I CCWGG 3 cut(s) 744, 1735, 2476
PspEI GGTNACC 1 cut(s) 3365
PspGI CCWGG 3 cut(s) 744, 1735, 2476
PspN4I GGNNCC 8 cut(s) 366, 480, 750, 1466, 1567, 2181, 3867, 4396
PspPI GGNCC 7 cut(s) 220, 328, 478, 1360, 1566, 2886, 3866
PspPPI RGGWCCY 1 cut(s) 1360
PstI CTGCAG 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 1612
PvuII CAGCTG 1 cut(s) 4264
RsaI GTAC 7 cut(s) 153, 1757, 2158, 2181, 2455, 2516, 3634
RsaNI GTAC 7 cut(s) 152, 1756, 2157, 2180, 2454, 2515, 3633
RseI CAYNNNNRTG 3 cut(s) 2312, 3620, 4373
SacII CCGCGG 1 cut(s) 431
SalI GTCGAC 1 cut(s) 2699
SatI GCNGC 9 cut(s) 171, 295, 298, 606, 955, 1320, 1589, 2207, 4218
Sau96I GGNCC 7 cut(s) 220, 328, 478, 1360, 1566, 2886, 3866
ScaI AGTACT 2 cut(s) 2158, 3634
SchI GAGTC 3 cut(s) 1000, 1749, 3233
ScrFI CCNGG 4 cut(s) 482, 746, 1737, 2478
SduI GDGCHC 4 cut(s) 47, 1663, 2561, 2642
SfaNI GCATC 9 cut(s) 866, 907, 1090, 1522, 2395, 2671, 2776, 4026, 4377
SfcI CTRYAG 3 cut(s) 295, 1596, 1647
Sfr274I CTCGAG 1 cut(s) 1583
Sfr303I CCGCGG 1 cut(s) 431
SgrBI CCGCGG 1 cut(s) 431
SinI GGWCC 5 cut(s) 220, 328, 1360, 1566, 3866
SlaI CTCGAG 1 cut(s) 1583
SmiMI CAYNNNNRTG 3 cut(s) 2312, 3620, 4373
SpeI ACTAGT 1 cut(s) 4339
SphI GCATGC 1 cut(s) 4255
SseBI AGGCCT 1 cut(s) 3762
SsiI CCGC 5 cut(s) 331, 428, 430, 605, 3113
SspI AATATT 4 cut(s) 458, 1371, 2332, 4015
StuI AGGCCT 1 cut(s) 3762
StyD4I CCNGG 4 cut(s) 480, 744, 1735, 2476
StyI CCWWGG 7 cut(s) 729, 1167, 1264, 2821, 3534, 3763, 4409
TaiI ACGT 6 cut(s) 163, 844, 990, 1734, 2391, 3404
TatI WGTACW 4 cut(s) 2156, 2453, 2514, 3632
TauI GCSGC 1 cut(s) 608
TscAI CASTG 4 cut(s) 2145, 3622, 3838, 3936
TseFI GTSAC 4 cut(s) 2355, 3225, 3365, 3845
TseI GCWGC 8 cut(s) 170, 294, 297, 954, 1319, 1588, 2206, 4217
Tsp45I GTSAC 4 cut(s) 2355, 3225, 3365, 3845
TspGWI ACGGA 2 cut(s) 1477, 1773
TspRI CASTG 4 cut(s) 2145, 3622, 3838, 3936
Van91I CCANNNNNTGG 3 cut(s) 309, 3517, 4298
VneI GTGCAC 1 cut(s) 43
VpaK11BI GGWCC 5 cut(s) 220, 328, 1360, 1566, 3866
XagI CCTNNNNNAGG 1 cut(s) 343
XbaI TCTAGA 3 cut(s) 2503, 3458, 4177
XceI RCATGY 4 cut(s) 294, 1754, 2521, 4255
XhoI CTCGAG 1 cut(s) 1583
XmaJI CCTAGG 1 cut(s) 3763
XmiI GTMKAC 4 cut(s) 1786, 2619, 2700, 3604
XmnI GAANNNNTTC 4 cut(s) 1677, 1731, 1848, 4306
ZraI GACGTC 1 cut(s) 2389
ZrmI AGTACT 2 cut(s) 2158, 3634
Zsp2I ATGCAT 2 cut(s) 2010, 2410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.