Rh6BG261200

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000203
Physical Location & Seq
Reverse (-)
47571368 .. 47577646
6279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG261200.1

Sequence Viewer

Length: 4344 bp
ATGGCAGATATATGTATTTCAGTTGTTGCAAAATTAGCAGAGTACACAGTCACACCTGTTCTGCGACAGTTTGGTTGTCTGATTAACTACAAGAGAAATGTTCAAGATCTCACCGAGAAGGTTACAACTTTGACTTCCAAAAGAGATGGGGTACAACTACAACTGGAAGCAGCAAACAATAACTTACAGGCTATTCTTCCCCAAGTTCAGGACTGGTTGGACCGAGTAAACCGGACCATACAAGAAAAGGAGACATGTTTTGAACAAGGAAGAGTAGCCAAGGCAACAACATGCTGCAGCTCCAATGGGTGGTGTCCAAATCTGAAGGTTCGGCATTCCTTGAGTAGGAAAGCAGCGAAGATGGCTCCAAAGGTAGATGTCCTCCTTACAGAAGGAGATTTCGGGACAATATCATGTCCTGCTCGTGCGCAGAAGATTGATTTTCCAGCCATGCGATATTTTGAGGATTCCACTGAGGCACCGGCCATGCCACATGAAGGCGCTAGCTCCTCCTCAGGAACATCCGATCCTGCTAAACCACCTGCACTTATGGATCTCTCTGAGCGTCTAAAATATAGGTTGCCTTATACAAAGGATCTTTTGACGGCTCTGCAAGATGACAAGATCAGTATGATTGGCATTTGTGGCACAATCAGAGAGATAGATACAGTAACAACTAAAGAGTTCATGAAAAGAATGAAACAAAAAAAGCTCTTCGAAAAGGTTTCGATGGCAGTTGTGTCCCAGGGGCCAGACTTGAAACGAATACAAGGTGAAATTGCAGAAATGCTAGACCTGAAATTGGAGAATGAGAGTTTGGTTGAACGGGCAGAGTGGCTACGTGCTGTTTTACAGGCATCCGATTCTAAGAGGATTCTTGTAATACTGACTGATGTCTGTGGAGTGCCTGATTTGGAAGCAATTGGAATTTCTCATGTTGATATCAAGAAAAGCTGCAAGATCATGTTGATTTCACAACTATCAAATGTGTTCAGTGAAATGGGGACTCAGAGCAATTTCGAATTGTTATCACAATCCACTCCCATAGCAAGACCTGTTGAGTTTGAATCAAGAATTTCAGTCATCAGAGATGTAATGGATGCTCTGACAGATGATGAATCCAACCCCATTGTAATATGTGGCATGGGGGGAATTGGCAAAACAACCTTGGTGATGGAAATTGGTGCAAAAGCGAGTGAAGTAGATCTATTTGATGATGTTGCAATTGCAGAGTTTACTCAAGAACCTGACTTGATCAAAATCCAAGGTAAAATTGCTAATGCTCTAGGTCTGGAACTTAAAGCAGGGGATGATAGAGCAGCCAAGTTACGAGAAAGATTATCGGGAGCTGCAAAAAGGAAACTTGTGATATTGGATAATGTTTGGACGCAACTAGATTTGTGGGATCTAGGGATTCCAATTTCCCGTGATCCAAAGAGTTGTAAACTTTTGGTGTCATCACGCAACCAAGATATCTTCAAAGAGATAAAAACCAAAAAGAATTTTCCCATTGATGGTTTGCCAAAACACGATGCTTGGAGTTTGTTTAAAAAGGTAGCAGGAGGTTCCATTGAATTCGATCACGAGCTGCGTCCTATAGCAGAACAGGTTCTGGATGAATGTGGTGGGTTACCAGTTGCAATTTCTACGGTTGGAAGGGCACTACAAGGTAAAGAGATTTCAAATTGGAGAAACGCACTCAGACAACTAAAACAAGCTTGCCCAGAAGACGTTCCAGGAGTGATAGAGCATGTGTATAAGAAAATAGAGTTCAGCTATAATTGTTTACCAAGCGAGCAAGTCAAATCATGTTTTTTGCTATGTTGTCTATTCCCAGAGAGTGAAGAATTTTCAACTGGATTGTTGGTTGCTTTTGGGATTGGGCTTGAGCTTTTTAAACGCATTGATTCAATATCTGAAGGAAGGGACTGCATAAAAGCTTTGCTTGACACACTCAAAGATCGTTCTTTATTGTTAGACGGTGATGAGATAGACAGCTTTAAAATGCATGATGTGGTGCGCGATGTAGCCGTGTATATAGGCTCGGAAGAGTTGAGACGGGAGAAGAATGATCGGAGGAGAGTTGTGCTAAGACAGGGAGAAGAATTGAACCATTGGCGCAAGAATAACGTTAACAAGGAATCTATCTATTGTAGTTCACTGACTACCAATATGAGTACTACTGAGCTTATACTGTTGATATCCACTGATGGAAGTAAACTGGAGCTGCCAGCCACAATTTTCAATGGAATGGAAGATCTCAAGGTTTTATTCATTACAAGTTCAATCCTCTCATCCCTTCAACTTTTGAAAAACCTTCAAACACTTGTTCTACTAAACTGCCAGCTTTTTAATATTGATGTCATTGGAGAGCTACGGACACTCGTGATGCTTAGCTTACGTGGATCTGACATCAAACAGTTGCCTGATACATTCAAACATTTATCTAATCTGAGGGGGTTAGAGTTGTCGAAATGTACGGAGCTTAAAGTAATCTCACCCGGTGTTATATCGAGTTTATCTCGTCTAGAAGAATTGTACATGTGGGATAGCTTTGAAGGTTGGGTGATTGAGAAACTAGGCTCAACAGAAACAATCAATTGGATCTCTCAATTTGAAGCTGATGCCCAACCAGAAGAAGTCAACATGTGGAAGAAATTCAACAGGTTGGCGGTTGAGAAACTAGCCTCAACAGAAACAACAACTGATCAGGTCTCTCAACGGGAGGAGGATACGGAAAGAAGTGAACCAGAGGAACACGACAACCTCGGAGATCTGTTCGCAAAGCATGTTCGAGGTTTACGGGGAAACTACGACCATCTCACAAGGTGGGCCAACTCTATGGACTGGAGAGTTGGGTTACTTTCAGAGCTACTTTCCTTGTCCTGTTTGACTACTTTGCAAGTGGTTTTACCACCAATTGACATTCTGCGAACCAGTAAGCTATTTCATAAGCTTGAAAGATTTAAGATCAATATAGGATGGAATGAGCATGAAGACTTCGAGGACGTTAAGGGTAACTACTTGCGAGTTCATGATCTTGAAGCAAGCTCTCTTGCGGATACTGGAATCGGTTTGTTGTTAAAGAAAACTAACAAGCTCGAGTTAAAAATGAAGAATTTGACCGACCCTCTCAATGTCTTAGATTTAAACTGCAGTCCAAACTTGGAGTCGCTTGCCCTTAAAGACTGTGATTCTCTGGAATATCTGATTGACATGACTGTTAAGCAAGATACTCTGCGAAGCGTCTTTCCTGTCTTGAATTATCTGAAAATCCGTAGAGCAAGGAGGCTGAAAGAGATTTTTCATGGTGAACTGCCACAAGAGTCGCTTCAGAAACTAGAAAGGCTAACCTTACGGCATTGCCAGTCATTGGAAGCAATTTTTGCCTTGGAAGGATCGGGACATCACGTCAAAGAGATCAACTTCCTTAGTTTGAGGTGGTTGAAACTTGAAGGTCTACCAAGCTTCACTGGAATGAGCAAAAGTACTTGTAAGGGGATGGAAAGGCAACTGGAAGTCAGCAGTCTCCATCACAATAGTTTGACCGTTGAACATTCACTTTTCTCTGACCCCAAGGTTGTCTTTCCCGTCTTGGAATCCCTGACAATTAGCCGTCTGGGCAACTTGAAAGAGATATGGAATAGCCGACTTTCACCCAACTCCTTCTCTGAATTAAGAAGGCTCACTGTCGAGGATTGTGACAAACTATTGCATTTGGTACCAACACAAATGCAAAATAGATTGCAGAGATTGGAGACCATACGGGTAGTCAACTGTTCTTCACTGGAAGAGATTTTTGAAGTAGGAAGATTGACCGTCAATGAAGGGAATGCTTCAATTTCTCAGTCAGACAAAATACCTTCAAATATTTCCCAACCTGATCAAGAGATGCAAATAAATGATATCATGGATTTCAAACAATCTTGTCAAGGCTTTCAGAATTTAACCAGACTATATGTCAGATCTTGTGGCAGTTTGAGATACCTGCTCTCTCCTTCGATTGCCAGAGGTCTTGTCAAGCTCGAACGTCTAGCTATAAGCTCTTGTGAGATGATCGAAGCAATAGTCGCAGCAGATGAGGGTGAAGAAACAGGGGATGAAAGCATGCTCCCTCAGCTTTTTGATTTGTCTCTTGTAGATCTACCCAATCTTGGAAGCTTTTCGCAAGGCAAATATACTTTTAATTGGCCACTGGTGAGATGGATCGAAATTAACAAATGCAACAAAATGAACAACTTCTGTCCAGGATCCCTCAGCATCTCAAGGGAAGTGTGGATATATGTCTATGATTCTGGTGAGAATCTCGAGCAGGAGCTGAATGATAGCAAGAAAGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1447

Amino Acids

163.2

Weight (kDa)

5.5

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 211 - 338 1.8e-08 NB-ARC domain
NB-ARC PF00931 362 - 520 7.2e-22 NB-ARC domain
LRR_8 PF13855 794 - 848 4e-06 Leucine rich repeat
LRR_RPS2 PF23247 1082 - 1158 1.6e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1201 - 1351 8.7e-28 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 550
Acc16I TGCGCA 1 cut(s) 429
Acc36I ACCTGC 2 cut(s) 550, 3996
Acc65I GGTACC 1 cut(s) 3721
AccB1I GGYRCC 2 cut(s) 478, 3721
AccB7I CCANNNNNTGG 2 cut(s) 309, 3373
AccI GTMKAC 1 cut(s) 3460
AccII CGCG 1 cut(s) 2022
AciI CCGC 2 cut(s) 2672, 3059
AclI AACGTT 1 cut(s) 2130
AcoI YGGCCR 2 cut(s) 483, 4190
AcsI RAATTY 8 cut(s) 927, 1074, 1501, 1574, 1847, 2657, 3118, 3943
AcuI CTGAAG 3 cut(s) 344, 1938, 3317
AdeI CACNNNGTG 2 cut(s) 2504, 2829
AfaI GTAC 7 cut(s) 44, 153, 2179, 2479, 2540, 3490, 3723
AfiI CCNNNNNNNGG 8 cut(s) 208, 309, 345, 497, 802, 1514, 3373, 4154
AflIII ACRYGT 3 cut(s) 254, 2541, 2646
AhdI GACNNNNNGTC 2 cut(s) 3410, 3959
AjiI CACGTC 1 cut(s) 3412
AjnI CCWGG 3 cut(s) 744, 1735, 4246
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
Alw26I GTCTC 6 cut(s) 245, 2050, 2719, 3533, 3752, 4137
AlwNI CAGNNNCTG 2 cut(s) 1612, 4318
Ama87I CYCGRG 2 cut(s) 3101, 4307
AoxI GGCC 4 cut(s) 483, 749, 2832, 4190
ApoI RAATTY 8 cut(s) 927, 1074, 1501, 1574, 1847, 2657, 3118, 3943
ArsI GACNNNNNNTTYG 4 cut(s) 3155, 3187, 4053, 4085
Asp700I GAANNNNTTC 5 cut(s) 1608, 1731, 2657, 4162, 4238
Asp718I GGTACC 1 cut(s) 3721
AspLEI GCGC 4 cut(s) 430, 503, 2022, 2121
AspS9I GGNCC 4 cut(s) 220, 234, 749, 2832
AsuC2I CCSGG 1 cut(s) 2502
AsuII TTCGAA 2 cut(s) 717, 1020
AsuNHI GCTAGC 1 cut(s) 503
AvaI CYCGRG 2 cut(s) 3101, 4307
AvaII GGWCC 2 cut(s) 220, 234
AxyI CCTNAGG 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 1663
BalI TGGCCA 1 cut(s) 4192
BamHI GGATCC 1 cut(s) 4250
BanI GGYRCC 2 cut(s) 478, 3721
BauI CACGAG 3 cut(s) 423, 1583, 2384
BbsI GAAGAC 2 cut(s) 1734, 3003
BbvCI CCTCAGC 2 cut(s) 4116, 4256
BceAI ACGGC 4 cut(s) 621, 2015, 3374, 3600
BciT130I CCWGG 3 cut(s) 746, 1737, 4248
BciVI GTATCC 2 cut(s) 2725, 3055
BclI TGATCA 3 cut(s) 1254, 2707, 3883
BcnI CCSGG 1 cut(s) 2502
BcoDI GTCTC 6 cut(s) 245, 2050, 2719, 3533, 3752, 4137
BfmI CTRYAG 3 cut(s) 295, 1596, 3154
BfoI RGCGCY 1 cut(s) 504
BfuAI ACCTGC 2 cut(s) 550, 3996
BfuI GTATCC 2 cut(s) 2725, 3055
BglI GCCNNNNNGGC 1 cut(s) 3621
BglII AGATCT 6 cut(s) 106, 1204, 2257, 2773, 3965, 4141
BlpI GCTNAGC 1 cut(s) 2393
BmcAI AGTACT 2 cut(s) 2179, 3490
Bme1390I CCNGG 4 cut(s) 746, 1737, 2502, 4248
Bme18I GGWCC 2 cut(s) 220, 234
BmeRI GACNNNNNGTC 2 cut(s) 3410, 3959
BmeT110I CYCGRG 2 cut(s) 3101, 4307
BmgBI CACGTC 1 cut(s) 3412
BmgT120I GGNCC 4 cut(s) 220, 234, 749, 2832
BmiI GGNNCC 6 cut(s) 366, 480, 750, 1567, 3723, 4252
BmrFI CCNGG 4 cut(s) 746, 1737, 2502, 4248
BmsI GCATC 7 cut(s) 866, 1090, 1522, 2379, 2614, 3882, 4269
BmtI GCTAGC 1 cut(s) 507
BoxI GACNNNNGTC 1 cut(s) 893
BpiI GAAGAC 2 cut(s) 1734, 3003
BplI GAGNNNNNCTC 2 cut(s) 2506, 2538
BpmI CTGGAG 2 cut(s) 2243, 2869
Bpu10I CCTNAGC 2 cut(s) 4116, 4256
Bpu1102I GCTNAGC 1 cut(s) 2393
Bpu14I TTCGAA 2 cut(s) 717, 1020
BpuEI CTTGAG 5 cut(s) 361, 1224, 1907, 2246, 4249
BpuMI CCSGG 1 cut(s) 2502
BsaAI YACGTR 2 cut(s) 842, 2402
BsaBI GATNNNNATC 1 cut(s) 1259
BsaI GGTCTC 2 cut(s) 2719, 3752
BsaJI CCNNGG 8 cut(s) 279, 744, 745, 1167, 1264, 2767, 3390, 3576
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 8 cut(s) 208, 309, 345, 497, 802, 1514, 3373, 4154
Bse118I RCCGGY 1 cut(s) 481
Bse21I CCTNAGG 1 cut(s) 514
Bse3DI GCAATG 1 cut(s) 3361
Bse8I GATNNNNATC 1 cut(s) 1259
BseBI CCWGG 3 cut(s) 746, 1737, 4248
BseDI CCNNGG 8 cut(s) 279, 744, 745, 1167, 1264, 2767, 3390, 3576
BseGI GGATG 9 cut(s) 521, 857, 1105, 1315, 1621, 2294, 2987, 3507, 4105
BseJI GATNNNNATC 1 cut(s) 1259
BseLI CCNNNNNNNGG 8 cut(s) 208, 309, 345, 497, 802, 1514, 3373, 4154
BseMI GCAATG 1 cut(s) 3361
BseRI GAGGAG 4 cut(s) 499, 502, 2092, 2741
BseSI GKGCMC 1 cut(s) 1663
BsgI GTGCAG 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 2022
BshFI GGCC 4 cut(s) 485, 751, 2834, 4192
BshNI GGYRCC 2 cut(s) 478, 3721
BsiHKCI CYCGRG 2 cut(s) 3101, 4307
BsiSI CCGG 3 cut(s) 232, 482, 2502
BslFI GGGAC 5 cut(s) 418, 727, 1018, 1940, 3417
BslI CCNNNNNNNGG 8 cut(s) 208, 309, 345, 497, 802, 1514, 3373, 4154
BsmAI GTCTC 6 cut(s) 245, 2050, 2719, 3533, 3752, 4137
BsmBI CGTCTC 1 cut(s) 2050
BsmFI GGGAC 5 cut(s) 418, 727, 1018, 1940, 3417
BsmI GAATGC 2 cut(s) 334, 3838
BsnI GGCC 4 cut(s) 485, 751, 2834, 4192
Bso31I GGTCTC 2 cut(s) 2719, 3752
BsoBI CYCGRG 2 cut(s) 3101, 4307
Bsp119I TTCGAA 2 cut(s) 717, 1020
Bsp1286I GDGCHC 1 cut(s) 1663
Bsp1407I TGTACA 1 cut(s) 2538
Bsp1720I GCTNAGC 1 cut(s) 2393
BspACI CCGC 2 cut(s) 2672, 3059
BspANI GGCC 4 cut(s) 485, 751, 2834, 4192
BspFNI CGCG 1 cut(s) 2022
BspHI TCATGA 2 cut(s) 687, 3034
BspLI GGNNCC 6 cut(s) 366, 480, 750, 1567, 3723, 4252
BspMAI CTGCAG 2 cut(s) 299, 3158
BspMI ACCTGC 2 cut(s) 550, 3996
BspOI GCTAGC 1 cut(s) 507
BspQI GCTCTTC 1 cut(s) 719
BspT104I TTCGAA 2 cut(s) 717, 1020
BspT107I GGYRCC 2 cut(s) 478, 3721
BspTNI GGTCTC 2 cut(s) 2719, 3752
BsrDI GCAATG 1 cut(s) 3361
BsrFI RCCGGY 1 cut(s) 481
BsrGI TGTACA 1 cut(s) 2538
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 8 cut(s) 279, 744, 745, 1167, 1264, 2767, 3390, 3576
BssSI CACGAG 3 cut(s) 423, 1583, 2384
BssT1I CCWWGG 5 cut(s) 279, 1167, 1264, 3390, 3576
Bst2BI CACGAG 3 cut(s) 423, 1583, 2384
Bst2UI CCWGG 3 cut(s) 746, 1737, 4248
Bst6I CTCTTC 4 cut(s) 265, 719, 2043, 3786
BstAPI GCANNNNNTGC 1 cut(s) 3386
BstAUI TGTACA 1 cut(s) 2538
BstBAI YACGTR 2 cut(s) 842, 2402
BstBI TTCGAA 2 cut(s) 717, 1020
BstC8I GCNNGC 8 cut(s) 505, 1720, 1796, 2232, 2345, 3049, 3177, 4109
BstEII GGTNACC 1 cut(s) 1629
BstENI CCTNNNNNAGG 1 cut(s) 343
BstF5I GGATG 9 cut(s) 521, 857, 1105, 1315, 1621, 2294, 2987, 3507, 4105
BstFNI CGCG 1 cut(s) 2022
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 4 cut(s) 430, 503, 2022, 2121
BstMAI GTCTC 6 cut(s) 245, 2050, 2719, 3533, 3752, 4137
BstMWI GCNNNNNNNGC 7 cut(s) 35, 362, 645, 3386, 3621, 4070, 4117
BstNI CCWGG 3 cut(s) 746, 1737, 4248
BstNSI RCATGY 7 cut(s) 258, 294, 1754, 2545, 2650, 2792, 4111
BstPAI GACNNNNGTC 1 cut(s) 893
BstPI GGTNACC 1 cut(s) 1629
BstSCI CCNGG 4 cut(s) 744, 1735, 2500, 4246
BstSFI CTRYAG 3 cut(s) 295, 1596, 3154
BstSLI GKGCMC 1 cut(s) 1663
BstUI CGCG 1 cut(s) 2022
BstV2I GAAGAC 2 cut(s) 1734, 3003
BstXI CCANNNNNNTGG 1 cut(s) 2842
Bsu36I CCTNAGG 1 cut(s) 514
BsuI GTATCC 2 cut(s) 2725, 3055
BsuRI GGCC 4 cut(s) 485, 751, 2834, 4192
BtgZI GCGATG 1 cut(s) 2037
BtrI CACGTC 1 cut(s) 3412
BtsCI GGATG 9 cut(s) 521, 857, 1105, 1315, 1621, 2294, 2987, 3507, 4105
BtsIMutI CAGTG 8 cut(s) 471, 1000, 2159, 2205, 3471, 3687, 3785, 4193
BveI ACCTGC 2 cut(s) 550, 3996
Cac8I GCNNGC 8 cut(s) 505, 1720, 1796, 2232, 2345, 3049, 3177, 4109
CaiI CAGNNNCTG 2 cut(s) 1612, 4318
CciI TCATGA 2 cut(s) 687, 3034
CfoI GCGC 4 cut(s) 430, 503, 2022, 2121
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 4 cut(s) 220, 234, 749, 2832
CseI GACGC 4 cut(s) 554, 1396, 1580, 3235
Csp6I GTAC 7 cut(s) 43, 152, 2178, 2478, 2539, 3489, 3722
CviQI GTAC 7 cut(s) 43, 152, 2178, 2478, 2539, 3489, 3722
DraI TTTAAA 4 cut(s) 1549, 1897, 2002, 3150
DraIII CACNNNGTG 2 cut(s) 2504, 2829
DriI GACNNNNNGTC 2 cut(s) 3410, 3959
EaeI YGGCCR 2 cut(s) 483, 4190
Eam1104I CTCTTC 4 cut(s) 265, 719, 2043, 3786
Eam1105I GACNNNNNGTC 2 cut(s) 3410, 3959
EarI CTCTTC 4 cut(s) 265, 719, 2043, 3786
Eco130I CCWWGG 5 cut(s) 279, 1167, 1264, 3390, 3576
Eco31I GGTCTC 2 cut(s) 2719, 3752
Eco32I GATATC 4 cut(s) 943, 1474, 2202, 3907
Eco47I GGWCC 2 cut(s) 220, 234
Eco57I CTGAAG 3 cut(s) 344, 1938, 3317
Eco81I CCTNAGG 1 cut(s) 514
Eco88I CYCGRG 2 cut(s) 3101, 4307
Eco91I GGTNACC 1 cut(s) 1629
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoO65I GGTNACC 1 cut(s) 1629
EcoRI GAATTC 1 cut(s) 1574
EcoRII CCWGG 3 cut(s) 744, 1735, 4246
EcoRV GATATC 4 cut(s) 943, 1474, 2202, 3907
EcoT14I CCWWGG 5 cut(s) 279, 1167, 1264, 3390, 3576
EcoT22I ATGCAT 1 cut(s) 2010
ErhI CCWWGG 5 cut(s) 279, 1167, 1264, 3390, 3576
Esp3I CGTCTC 1 cut(s) 2050
FalI AAGNNNNNCTT 6 cut(s) 1929, 1961, 3315, 3347, 3569, 3601
FaqI GGGAC 5 cut(s) 418, 727, 1018, 1940, 3417
FbaI TGATCA 3 cut(s) 1254, 2707, 3883
FblI GTMKAC 1 cut(s) 3460
FokI GGATG 9 cut(s) 508, 844, 1112, 1322, 1628, 2281, 2994, 3514, 4112
FspI TGCGCA 1 cut(s) 429
GlaI GCGC 4 cut(s) 429, 502, 2021, 2120
GsuI CTGGAG 2 cut(s) 2243, 2869
HaeII RGCGCY 1 cut(s) 504
HaeIII GGCC 4 cut(s) 485, 751, 2834, 4192
HapII CCGG 3 cut(s) 232, 482, 2502
HgaI GACGC 4 cut(s) 554, 1396, 1580, 3235
HhaI GCGC 4 cut(s) 430, 503, 2022, 2121
Hin6I GCGC 4 cut(s) 428, 501, 2020, 2119
HinP1I GCGC 4 cut(s) 428, 501, 2020, 2119
HincII GTYRAC 3 cut(s) 2134, 2644, 3775
HindII GTYRAC 3 cut(s) 2134, 2644, 3775
HindIII AAGCTT 5 cut(s) 1716, 1938, 2954, 3466, 4159
HpaI GTTAAC 1 cut(s) 2134
HpaII CCGG 3 cut(s) 232, 482, 2502
HpyCH4IV ACGT 7 cut(s) 841, 1731, 2130, 2401, 3009, 3411, 4030
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 362, 645, 3386, 3621, 4070, 4117
HpySE526I ACGT 7 cut(s) 841, 1731, 2130, 2401, 3009, 3411, 4030
HspAI GCGC 4 cut(s) 428, 501, 2020, 2119
KpnI GGTACC 1 cut(s) 3725
Ksp22I TGATCA 3 cut(s) 1254, 2707, 3883
KspAI GTTAAC 1 cut(s) 2134
LguI GCTCTTC 1 cut(s) 719
LmnI GCTCC 8 cut(s) 305, 370, 512, 1346, 2224, 2482, 4116, 4315
LweI GCATC 7 cut(s) 866, 1090, 1522, 2379, 2614, 3882, 4269
MaeII ACGT 7 cut(s) 841, 1731, 2130, 2401, 3009, 3411, 4030
MaeIII GTNAC 8 cut(s) 49, 121, 670, 1326, 1629, 2859, 3017, 3701
MfeI CAATTG 4 cut(s) 921, 1224, 2599, 2919
MhlI GDGCHC 1 cut(s) 1663
MlsI TGGCCA 1 cut(s) 4192
MluNI TGGCCA 1 cut(s) 4192
MlyI GAGTC 3 cut(s) 1000, 3179, 3335
MmeI TCCRAC 3 cut(s) 198, 1146, 1633
Mox20I TGGCCA 1 cut(s) 4192
Mph1103I ATGCAT 1 cut(s) 2010
MroXI GAANNNNTTC 5 cut(s) 1608, 1731, 2657, 4162, 4238
MscI TGGCCA 1 cut(s) 4192
MslI CAYNNNNRTG 1 cut(s) 3476
Msp20I TGGCCA 1 cut(s) 4192
MspI CCGG 3 cut(s) 232, 482, 2502
MspR9I CCNGG 4 cut(s) 746, 1737, 2502, 4248
MunI CAATTG 4 cut(s) 921, 1224, 2599, 2919
Mva1269I GAATGC 2 cut(s) 334, 3838
MvaI CCWGG 3 cut(s) 746, 1737, 4248
MvnI CGCG 1 cut(s) 2022
MwoI GCNNNNNNNGC 7 cut(s) 35, 362, 645, 3386, 3621, 4070, 4117
NciI CCSGG 1 cut(s) 2502
NheI GCTAGC 1 cut(s) 503
NlaIV GGNNCC 6 cut(s) 366, 480, 750, 1567, 3723, 4252
NmuCI GTSAC 2 cut(s) 49, 3701
NsbI TGCGCA 1 cut(s) 429
NsiI ATGCAT 1 cut(s) 2010
NspI RCATGY 7 cut(s) 258, 294, 1754, 2545, 2650, 2792, 4111
NspV TTCGAA 2 cut(s) 717, 1020
PaeI GCATGC 1 cut(s) 4111
PaeR7I CTCGAG 2 cut(s) 3101, 4307
PagI TCATGA 2 cut(s) 687, 3034
PaqCI CACCTGC 1 cut(s) 550
PasI CCCWGGG 1 cut(s) 745
PciI ACATGT 3 cut(s) 254, 2541, 2646
PciSI GCTCTTC 1 cut(s) 719
PctI GAATGC 2 cut(s) 334, 3838
PdmI GAANNNNTTC 5 cut(s) 1608, 1731, 2657, 4162, 4238
PflMI CCANNNNNTGG 2 cut(s) 309, 3373
PfoI TCCNGGA 2 cut(s) 1735, 4246
PleI GAGTC 3 cut(s) 1000, 3178, 3334
PpsI GAGTC 3 cut(s) 1000, 3178, 3334
Ppu21I YACGTR 2 cut(s) 842, 2402
PscI ACATGT 3 cut(s) 254, 2541, 2646
PshAI GACNNNNGTC 1 cut(s) 893
Psp1406I AACGTT 1 cut(s) 2130
Psp6I CCWGG 3 cut(s) 744, 1735, 4246
PspEI GGTNACC 1 cut(s) 1629
PspGI CCWGG 3 cut(s) 744, 1735, 4246
PspN4I GGNNCC 6 cut(s) 366, 480, 750, 1567, 3723, 4252
PspPI GGNCC 4 cut(s) 220, 234, 749, 2832
PspXI VCTCGAGB 1 cut(s) 3101
PstI CTGCAG 2 cut(s) 299, 3158
PstNI CAGNNNCTG 2 cut(s) 1612, 4318
RsaI GTAC 7 cut(s) 44, 153, 2179, 2479, 2540, 3490, 3723
RsaNI GTAC 7 cut(s) 43, 152, 2178, 2478, 2539, 3489, 3722
RseI CAYNNNNRTG 1 cut(s) 3476
SapI GCTCTTC 1 cut(s) 719
Sau96I GGNCC 4 cut(s) 220, 234, 749, 2832
ScaI AGTACT 2 cut(s) 2179, 3490
SchI GAGTC 3 cut(s) 1000, 3179, 3335
ScrFI CCNGG 4 cut(s) 746, 1737, 2502, 4248
SduI GDGCHC 1 cut(s) 1663
SfaNI GCATC 7 cut(s) 866, 1090, 1522, 2379, 2614, 3882, 4269
SfcI CTRYAG 3 cut(s) 295, 1596, 3154
Sfr274I CTCGAG 2 cut(s) 3101, 4307
SfuI TTCGAA 2 cut(s) 717, 1020
SinI GGWCC 2 cut(s) 220, 234
SlaI CTCGAG 2 cut(s) 3101, 4307
SmiMI CAYNNNNRTG 1 cut(s) 3476
SmlI CTYRAG 7 cut(s) 340, 1239, 1886, 2261, 3101, 4264, 4307
SmoI CTYRAG 7 cut(s) 340, 1239, 1886, 2261, 3101, 4264, 4307
SphI GCATGC 1 cut(s) 4111
SsiI CCGC 2 cut(s) 2672, 3059
SspI AATATT 2 cut(s) 2356, 3871
StyD4I CCNGG 4 cut(s) 744, 1735, 2500, 4246
StyI CCWWGG 5 cut(s) 279, 1167, 1264, 3390, 3576
TaiI ACGT 7 cut(s) 844, 1734, 2133, 2404, 3012, 3414, 4033
TaqII GACCGA 2 cut(s) 237, 3140
TatI WGTACW 4 cut(s) 42, 2177, 2538, 3488
TscAI CASTG 8 cut(s) 478, 1000, 2166, 2212, 3478, 3694, 3792, 4200
TseFI GTSAC 2 cut(s) 49, 3701
Tsp45I GTSAC 2 cut(s) 49, 3701
TspGWI ACGGA 4 cut(s) 2392, 2495, 2750, 3266
TspRI CASTG 8 cut(s) 478, 1000, 2166, 2212, 3478, 3694, 3792, 4200
Van91I CCANNNNNTGG 2 cut(s) 309, 3373
VpaK11BI GGWCC 2 cut(s) 220, 234
XagI CCTNNNNNAGG 1 cut(s) 343
XapI RAATTY 8 cut(s) 927, 1074, 1501, 1574, 1847, 2657, 3118, 3943
XbaI TCTAGA 1 cut(s) 2527
XceI RCATGY 7 cut(s) 258, 294, 1754, 2545, 2650, 2792, 4111
XcmI CCANNNNNNNNNTGG 1 cut(s) 4200
XhoI CTCGAG 2 cut(s) 3101, 4307
XmiI GTMKAC 1 cut(s) 3460
XmnI GAANNNNTTC 5 cut(s) 1608, 1731, 2657, 4162, 4238
ZrmI AGTACT 2 cut(s) 2179, 3490
Zsp2I ATGCAT 1 cut(s) 2010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.