FvH4_2g36850

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000203
Physical Location & Seq
Forward (+)
26911258 .. 26919017
7760 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36850.t5

Sequence Viewer

Length: 3966 bp
ATGAAATGCCGCTTAAAGACTTCGACTCTCTCTCTCTCGTGGGCCACCGTGATAATGGGTTGTTATATCCCACAGTGCCAATTACATGCACTGAAAGAAATGGAAGAGACAGCTATTACAGAAGGTGCTGTGCCGGTTGCAAAATGGACAATAAAAAAACTTTGGCAAGAGTTGGGTTATATGTTTTGTTACAAAAGCAACATTGGGGATCTCAACGAGCAGGTTCAGCAGCTGCGTCACAAAAAAGATGGAATAGAGCTGGAGGAGAAAGGTGCGAGAGAAAATTCAATGATTATTGGTGGCCAAGTGATTGATTGGCTGGTCAAGGCAGACGAGCAAATAAAAGAAAAGGAGACGACTTTCAGTGAAAAAACTGTAGCAGCGAGGGCGGCATGCTGTAATGGATGGCTTCCAAATATGAAGGGTCGTTTTTCTCTGGGAAGGAATGCTAAGAAGATGACTGAAAATGTGAAAGACCTCCTTGGTAGTGAACCCAGGACTATAGCCCATCCTGCTCCTCACGCAAAGGTGGAATTCCGACCCACAGAGGACTCTACGCAGCTGGGAACCAACTTCCATGAAGGAGCCAGCTCCTCAGGAACATCAAACCATGATCAGCCACCTGCATTTGAGAGGAAAAATATTTATTTTGATTCAAGAAAATCAACCATCAGGAATGTGATGGAAGCTCTCAGGGGGAATCAAATCAATCCGATTATCATATGCGGCTTGGGCGGCATGGGCAAAACAACATTGATGGGGCAAGTTCTTGAGAAAGCAAAGGAAGAGGGTCTGTTCGATGAGTATACAAAGGCAACCGTTAAGGATTCTCTTAACAAAGCTGCTGACGTGATTCAGATTCAAGCTGAACTTGCAGAATATTTAGGCACGAAGCTCCTTGAGGACGTAAAAGAGAGTGATCCAAGAGCAAGTAAGCTACGTCAAAGATTATCTCAAGGCAACAAGAAGATCCTTGTAATGTTAGACAATGTTTGGACAACAACCCCAGACATGTTGTGGGACATAGGGATTCCCCCTAGTTGTCAACTTTTGGTGACATCAAGACAACAAGATGTATTCAAGGACATGGACAGACGACAGATTTTCCCAATTCATGGTTTAAGGGATCCTGATGCCTGGAGACTGTTTAAGGAGAGAGCAGGAAGTTGCATCGAATCTGATCCCGAGGTGCATGTTGTAGCCGATATGATCTTGCAGAAATGTGGTGGGTTGCCCATTGCAATTTCGACGGTTGGAACGGCACTGAAAGATGCAAGCATTGAAATTTGGAATGATGCCCTTGTGCAACTCGAAAATGCATGCCCAGATAACATAGATGGAGTGATAGATCATGTGTATGGGCCAATAACGTTCAGTTATGAGCGTTTACAAGGTGAGGAAGCCAAATCATGTTTTTTGCTGTGTTGCATGTTTGGAGGAAGCGCTGATATACGGATTGAAGATTTGGTCATGTATGGGCTTGGGCTTGAGCTCTTTAATTTTAAAGGCACTGATTCAATGGTTAAAGGAAGAAACCGTGTGAAATCATTGGTTCAGACACTCAAAAGTCGCTTTTTATTGTTAGAGAGCGATAAGAAAGAGTGTGTGAGAATGCATGATGTGGTGCGTGATGTTGCCTTACATATAGCTTCTGAAGAGTTTGTACAAAAGAAAAATGATGGGGTTGAAAAGAGAATCGTGGTCAGGGACAAAGCAGCAACTGTGAAATTGATGGATTGGCCTATTTCTGAGCAATCTGCTATTTGCAGGTCACTGTTTATTGGTGGGCGTTTCGTGCTTTTACAGTACATATCTGAAGTGGATCCGCCACACACAATTTTCAATAGAATGGAACATCTTAAGGTTCTAGCAGTTGGGTCGTGCCCTTCGTTACAACTGTCATCACTTGCAGTTTTGAGAGACATTCAAACACTACGTCTAGTGGACTGCAGTGTGGAGGATGTATCTGTCATTGGGGAGCTAAGTACACTCATGATACTCAGTTTGCGTGACTGTTCGAACATCGAGCAATTGCCTGATACATTTAAAAATTTGTCTAGGTTGAGATTGCTTGATTTGTCGGGGTGTAGGGAGCTCAAAATAATCTCACCAGGTGTGATAAAAAGTTTATTCCGACTAGAAGAACTGTACATGTGGGATAGCTTCCAGGATTGGGTGGTGGATAAACTAGCCTCCCCCGAAACAGCCAACTGGATCTCTCAACTGGAGGCTGAGGGAAGAGCTCGGCGATTCAGATCTTGGGTACTTAAAACGCTGGACACGTGGAAGAGTCTTAACCGATCGGCGGTTGAGCAACCAGCAGACGTCGGAAAGCTGTTCAGACAGGAGATTGGACGTGTGCGTGTACAGGAGGAGAGCACAAGGTGGGCAAACTCCATGGACTGGAACACTGGGATACTTGCAGAGCTGCTTTCCTTGTCCCGTTTGACGACATTAGAAGCTGTTCTACCACCGGTTAATATACTTCTAACCAGCCCTCTCTTTAACAACCTCGAAAGATTTAAGATCACTATAGGGTGGGAGGAGCATATACGTGCTCGGGATTCAGAGAAATATGGGGTTGATGACAACTACTTGAGAGTTGGCAATCTTGATCCAAGCGCTATTGTGGGTAGTGGAATCACTCCGTTCCTAAAGAAAACCAGCATTATCGAGTTTCGAAGGACGAATTCAACTGAGCCTCAACTTATGAATGTCTTAGGTACGGTCTGCTTTGCAAAGATACGGTCTCTTGCACTCACGAGCTGTGAGGATGTGGAGTATCTGATTGACACGACTACTACTCCAAATAGCAGCAGCAGCAGCGCCATCTTTCCTCTCTTGAATGCACTGGAGATTAGTAATGCAATTAGCCTAAATGAGATTTTTCACGGTGAACTGCCAACGGGGTCTCTCAAGGAACTGCGAAGTTTGCGATTCTTTCTTCTACCAGAGTTGGCCTATATTTGGAAGACCAACAGGAAATCTGAATTAGGTGTCTTGAAAAACATTCTAGGACATGTTCAAGTGCTGGACGTAGGACACTGCTCGTCCTTGGAAGGGATTATTTCCTTTGAATCTGATGATGAGGAAGTGATCAACTTCCAGAGTCTGACAGAATTGTCTCTGTCAAAACTACCGTGCTTCATTAGGATAAGCCGAAAGATATCTAAGGGGCCGGAAAGACTCATCAAACAACCTCTGTTCGACACCAAGGTTCATTTCCCAGTCCTGACTAAACTACGCTTGGGCAACATGAACTTCAAAGAGATATGGAACAACCAACTTTCAGCGGAATCCTTTTGTGAACTAAAGTATCTACATGTGACAGCATGTAAGAAACTTTTGCATTTGGTACCAACACATATGCAAAATAGAATGCAGAAATTGGAGCGCATACATGCATGGTCATGTTCTTCACTAGAAGAGATATTTGAATTTAGAAGATTGATTGTCGATGATGAGGGGGATGCCACTGCTTTAACAATTTTTGAGTCAGGAAATCAAGGGACGCGAATAAACAAGATGATGTCCTTTAAAGAATCTCGTCAAGCCTTTCAGAATCTTAGAGAGATAGTGATTAAGCGTTGCGACAGTTTGAGAACTCTGCTCTCTCCCTCAGTTGCCAGAGGTCTCGTGAAGCTCCAAACACTAACAATAGAAAACTGCAAGAGGATTGAAGAGGTAGTAGCAGTACCAGCTGAGGGTGAAGAAACAGAGGATGACAACATGTTCCCTCAGCTGCAAACTTTGGAACTTGAGGATCTACCAAACCTCGGAAGCTTTTCACAAGGCATTTATAATTTCAAATGGCCACTAGTGAGAGCCATAATTATCCTCAGATGCAACAATATGAATAAGTTTTGTTTAGGAACCCTCAGCACACGGAAGAAAGTGGAAATTTTTGTCAAAGGTGCTGGTGAGAGTGTTTTGCAGCAGCTCAATAACAGTAGAAAAGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1322

Amino Acids

149.26

Weight (kDa)

6.49

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATPase_2 PF01637 186 - 298 6e-06 ATPase domain predominantly from Archaea
NB-ARC PF00931 188 - 354 1.8e-19 NB-ARC domain
LRR_14 PF23598 582 - 688 5.6e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 1046 - 1191 1.3e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1149 - 1251 1.3e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3801
AarI CACCTGC 1 cut(s) 631
AasI GACNNNNNNGTC 1 cut(s) 3121
AatII GACGTC 1 cut(s) 2328
Acc36I ACCTGC 3 cut(s) 211, 631, 1758
Acc65I GGTACC 1 cut(s) 3355
AccB1I GGYRCC 1 cut(s) 3355
AccB7I CCANNNNNTGG 2 cut(s) 1115, 2403
AccI GTMKAC 1 cut(s) 806
AccII CGCG 1 cut(s) 3514
AciI CCGC 7 cut(s) 10, 389, 726, 735, 1826, 2306, 3293
AclI AACGTT 1 cut(s) 1370
AcoI YGGCCR 2 cut(s) 301, 3812
AcsI RAATTY 7 cut(s) 283, 533, 1284, 2050, 2689, 3437, 3900
AcuI CTGAAG 2 cut(s) 1674, 1836
AcvI CACGTG 1 cut(s) 2283
AcyI GRCGYC 1 cut(s) 2325
AdeI CACNNNGTG 2 cut(s) 2114, 2385
AfaI GTAC 9 cut(s) 1665, 1808, 1987, 2150, 2265, 2367, 2725, 3357, 3696
AfeI AGCGCT 2 cut(s) 1444, 2623
AfiI CCNNNNNNNGG 8 cut(s) 1115, 2173, 2305, 2403, 2967, 3060, 3704, 3776
AflII CTTAAG 1 cut(s) 1859
AflIII ACRYGT 7 cut(s) 1011, 2151, 2280, 2356, 3019, 3322, 3729
AgeI ACCGGT 1 cut(s) 2473
AhlI ACTAGT 1 cut(s) 3817
AjiI CACGTC 2 cut(s) 850, 2357
AjnI CCWGG 4 cut(s) 494, 1136, 2110, 2166
AjuI GAANNNNNNNTTGG 2 cut(s) 3206, 3238
Alw21I GWGCWC 5 cut(s) 1494, 2097, 2245, 2381, 2560
Alw26I GTCTC 8 cut(s) 101, 347, 1135, 1914, 2754, 2916, 3129, 3638
AlwNI CAGNNNCTG 3 cut(s) 232, 1721, 3112
Ama87I CYCGRG 2 cut(s) 1184, 2559
Aor51HI AGCGCT 2 cut(s) 1444, 2623
AoxI GGCC 7 cut(s) 42, 301, 1361, 1739, 2958, 3176, 3812
ApoI RAATTY 7 cut(s) 283, 533, 1284, 2050, 2689, 3437, 3900
ArsI GACNNNNNNTTYG 4 cut(s) 1921, 1953, 2732, 2764
AsiGI ACCGGT 1 cut(s) 2473
Asp700I GAANNNNTTC 2 cut(s) 2463, 3784
Asp718I GGTACC 1 cut(s) 3355
AspLEI GCGC 4 cut(s) 1445, 2624, 2828, 3396
AspS9I GGNCC 3 cut(s) 42, 1361, 3176
AsuHPI GGTGA 6 cut(s) 1066, 1406, 2100, 2906, 3719, 3932
AsuII TTCGAA 2 cut(s) 2018, 2680
AvaI CYCGRG 2 cut(s) 1184, 2559
AxyI CCTNAGG 1 cut(s) 595
BaeGI GKGCMC 1 cut(s) 1886
BaeI ACNNNNGTAYC 2 cut(s) 3299, 3332
BalI TGGCCA 2 cut(s) 303, 3814
BamHI GGATCC 2 cut(s) 1126, 1822
BanI GGYRCC 1 cut(s) 3355
BanII GRGCYC 3 cut(s) 1494, 2097, 2245
BarI GAAGNNNNNNTAC 4 cut(s) 1647, 1679, 2451, 2483
BauI CACGAG 3 cut(s) 37, 2761, 3635
BbrPI CACGTG 1 cut(s) 2283
BbsI GAAGAC 1 cut(s) 2978
Bbv12I GWGCWC 5 cut(s) 1494, 2097, 2245, 2381, 2560
BbvCI CCTCAGC 4 cut(s) 2232, 3702, 3738, 3878
BceAI ACGGC 1 cut(s) 1275
BcgI CGANNNNNNTGC 2 cut(s) 1860, 1894
BciT130I CCWGG 4 cut(s) 496, 1138, 2112, 2168
BciVI GTATCC 1 cut(s) 2409
BclI TGATCA 2 cut(s) 613, 3096
BcoDI GTCTC 8 cut(s) 101, 347, 1135, 1914, 2754, 2916, 3129, 3638
BcuI ACTAGT 1 cut(s) 3817
BfaI CTAG 9 cut(s) 1038, 1868, 1940, 2058, 2138, 2189, 3014, 3422, 3818
BfmI CTRYAG 4 cut(s) 375, 501, 1948, 2532
BfoI RGCGCY 3 cut(s) 1446, 2625, 2829
BfrI CTTAAG 1 cut(s) 1859
BfuAI ACCTGC 3 cut(s) 211, 631, 1758
BfuI GTATCC 1 cut(s) 2409
BglII AGATCT 1 cut(s) 2255
Bme1390I CCNGG 4 cut(s) 496, 1138, 2112, 2168
BmeT110I CYCGRG 2 cut(s) 1184, 2559
BmgBI CACGTC 2 cut(s) 850, 2357
BmgT120I GGNCC 3 cut(s) 42, 1361, 3176
BmiI GGNNCC 7 cut(s) 568, 586, 1128, 1824, 3177, 3357, 3874
BmrFI CCNGG 4 cut(s) 496, 1138, 2112, 2168
BmrI ACTGGG 2 cut(s) 2421, 3221
BmsI GCATC 6 cut(s) 1123, 1179, 1261, 1285, 3460, 3833
BmuI ACTGGG 2 cut(s) 2421, 3221
BpiI GAAGAC 1 cut(s) 2978
BpmI CTGGAG 4 cut(s) 281, 1159, 2246, 2873
Bpu10I CCTNAGC 4 cut(s) 2232, 3702, 3738, 3878
Bpu14I TTCGAA 2 cut(s) 2018, 2680
BpuEI CTTGAG 7 cut(s) 791, 920, 939, 1508, 2617, 2900, 3779
BsaAI YACGTR 2 cut(s) 2283, 2555
BsaBI GATNNNNATC 1 cut(s) 2254
BsaHI GRCGYC 1 cut(s) 2325
BsaI GGTCTC 3 cut(s) 2754, 2916, 3638
BsaJI CCNNGG 7 cut(s) 481, 494, 1185, 2397, 3054, 3213, 3775
BsaWI WCCGGW 1 cut(s) 2473
BsaXI ACNNNNNCTCC 2 cut(s) 2788, 2818
Bsc4I CCNNNNNNNGG 8 cut(s) 1115, 2173, 2305, 2403, 2967, 3060, 3704, 3776
Bse118I RCCGGY 2 cut(s) 133, 2473
Bse1I ACTGG 6 cut(s) 2216, 2229, 2408, 2416, 2856, 3227
Bse21I CCTNAGG 1 cut(s) 595
Bse3DI GCAATG 1 cut(s) 1236
Bse8I GATNNNNATC 1 cut(s) 2254
BseBI CCWGG 4 cut(s) 496, 1138, 2112, 2168
BseDI CCNNGG 7 cut(s) 481, 494, 1185, 2397, 3054, 3213, 3775
BseGI GGATG 6 cut(s) 410, 508, 1966, 2779, 3475, 3727
BseJI GATNNNNATC 1 cut(s) 2254
BseLI CCNNNNNNNGG 8 cut(s) 1115, 2173, 2305, 2403, 2967, 3060, 3704, 3776
BseMI GCAATG 1 cut(s) 1236
BseNI ACTGG 6 cut(s) 2216, 2229, 2408, 2416, 2856, 3227
BseRI GAGGAG 5 cut(s) 278, 507, 583, 2387, 2558
BseSI GKGCMC 1 cut(s) 1886
BseYI CCCAGC 1 cut(s) 562
Bsh1236I CGCG 1 cut(s) 3514
Bsh1285I CGRYCG 1 cut(s) 2303
BshFI GGCC 7 cut(s) 44, 303, 1363, 1741, 2960, 3178, 3814
BshNI GGYRCC 1 cut(s) 3355
BshTI ACCGGT 1 cut(s) 2473
BsiEI CGRYCG 1 cut(s) 2303
BsiHKAI GWGCWC 5 cut(s) 1494, 2097, 2245, 2381, 2560
BsiHKCI CYCGRG 2 cut(s) 1184, 2559
BsiSI CCGG 3 cut(s) 134, 2474, 3179
BslFI GGGAC 4 cut(s) 1034, 1721, 2425, 3523
BslI CCNNNNNNNGG 8 cut(s) 1115, 2173, 2305, 2403, 2967, 3060, 3704, 3776
BsmAI GTCTC 8 cut(s) 101, 347, 1135, 1914, 2754, 2916, 3129, 3638
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 4 cut(s) 1034, 1721, 2425, 3523
BsmI GAATGC 4 cut(s) 451, 1617, 2851, 3384
BsnI GGCC 7 cut(s) 44, 303, 1363, 1741, 2960, 3178, 3814
Bso31I GGTCTC 3 cut(s) 2754, 2916, 3638
BsoBI CYCGRG 2 cut(s) 1184, 2559
Bsp119I TTCGAA 2 cut(s) 2018, 2680
Bsp1286I GDGCHC 6 cut(s) 1494, 1886, 2097, 2245, 2381, 2560
Bsp1407I TGTACA 3 cut(s) 1663, 2148, 2365
Bsp19I CCATGG 1 cut(s) 2397
BspACI CCGC 7 cut(s) 10, 389, 726, 735, 1826, 2306, 3293
BspANI GGCC 7 cut(s) 44, 303, 1363, 1741, 2960, 3178, 3814
BspFNI CGCG 1 cut(s) 3514
BspHI TCATGA 1 cut(s) 1992
BspLI GGNNCC 7 cut(s) 568, 586, 1128, 1824, 3177, 3357, 3874
BspMAI CTGCAG 1 cut(s) 1952
BspMI ACCTGC 3 cut(s) 211, 631, 1758
BspQI GCTCTTC 1 cut(s) 2233
BspT104I TTCGAA 2 cut(s) 2018, 2680
BspT107I GGYRCC 1 cut(s) 3355
BspTI CTTAAG 1 cut(s) 1859
BspTNI GGTCTC 3 cut(s) 2754, 2916, 3638
BsrDI GCAATG 1 cut(s) 1236
BsrFI RCCGGY 2 cut(s) 133, 2473
BsrGI TGTACA 3 cut(s) 1663, 2148, 2365
BsrI ACTGG 6 cut(s) 2216, 2229, 2408, 2416, 2856, 3227
BssAI RCCGGY 2 cut(s) 133, 2473
BssECI CCNNGG 7 cut(s) 481, 494, 1185, 2397, 3054, 3213, 3775
BssNAI GTATAC 1 cut(s) 807
BssNI GRCGYC 1 cut(s) 2325
BssSI CACGAG 3 cut(s) 37, 2761, 3635
BssT1I CCWWGG 4 cut(s) 481, 2397, 3054, 3213
Bst1107I GTATAC 1 cut(s) 807
Bst2BI CACGAG 3 cut(s) 37, 2761, 3635
Bst2UI CCWGG 4 cut(s) 496, 1138, 2112, 2168
Bst6I CTCTTC 7 cut(s) 99, 780, 1650, 2233, 2282, 3420, 3675
BstACI GRCGYC 1 cut(s) 2325
BstAFI CTTAAG 1 cut(s) 1859
BstAUI TGTACA 3 cut(s) 1663, 2148, 2365
BstBAI YACGTR 2 cut(s) 2283, 2555
BstBI TTCGAA 2 cut(s) 2018, 2680
BstC8I GCNNGC 4 cut(s) 394, 589, 1276, 1321
BstDSI CCRYGG 1 cut(s) 2397
BstF5I GGATG 6 cut(s) 410, 508, 1966, 2779, 3475, 3727
BstFNI CGCG 1 cut(s) 3514
BstH2I RGCGCY 3 cut(s) 1446, 2625, 2829
BstHHI GCGC 4 cut(s) 1445, 2624, 2828, 3396
BstMAI GTCTC 8 cut(s) 101, 347, 1135, 1914, 2754, 2916, 3129, 3638
BstMCI CGRYCG 1 cut(s) 2303
BstNI CCWGG 4 cut(s) 496, 1138, 2112, 2168
BstSCI CCNGG 4 cut(s) 494, 1136, 2110, 2166
BstSFI CTRYAG 4 cut(s) 375, 501, 1948, 2532
BstSLI GKGCMC 1 cut(s) 1886
BstUI CGCG 1 cut(s) 3514
BstV2I GAAGAC 1 cut(s) 2978
BstX2I RGATCY 7 cut(s) 208, 969, 1126, 1822, 2214, 2255, 3763
BstYI RGATCY 7 cut(s) 208, 969, 1126, 1822, 2214, 2255, 3763
BstZ17I GTATAC 1 cut(s) 807
Bsu36I CCTNAGG 1 cut(s) 595
BsuI GTATCC 1 cut(s) 2409
BsuRI GGCC 7 cut(s) 44, 303, 1363, 1741, 2960, 3178, 3814
BtgI CCRYGG 1 cut(s) 2397
BtrI CACGTC 2 cut(s) 850, 2357
BtsCI GGATG 6 cut(s) 410, 508, 1966, 2779, 3475, 3727
BtsI GCAGTG 3 cut(s) 1957, 3043, 3474
BveI ACCTGC 3 cut(s) 211, 631, 1758
Cac8I GCNNGC 4 cut(s) 394, 589, 1276, 1321
CaiI CAGNNNCTG 3 cut(s) 232, 1721, 3112
CciI TCATGA 1 cut(s) 1992
CfoI GCGC 4 cut(s) 1445, 2624, 2828, 3396
Cfr10I RCCGGY 2 cut(s) 133, 2473
Cfr13I GGNCC 3 cut(s) 42, 1361, 3176
CseI GACGC 2 cut(s) 224, 3520
CsiI ACCWGGT 1 cut(s) 2110
Csp6I GTAC 9 cut(s) 1664, 1807, 1986, 2149, 2264, 2366, 2724, 3356, 3695
CspAI ACCGGT 1 cut(s) 2473
CviQI GTAC 9 cut(s) 1664, 1807, 1986, 2149, 2264, 2366, 2724, 3356, 3695
DraI TTTAAA 3 cut(s) 1504, 2047, 3538
DraIII CACNNNGTG 2 cut(s) 2114, 2385
DrdI GACNNNNNNGTC 1 cut(s) 3121
DseDI GACNNNNNNGTC 1 cut(s) 3121
EaeI YGGCCR 2 cut(s) 301, 3812
Eam1104I CTCTTC 7 cut(s) 99, 780, 1650, 2233, 2282, 3420, 3675
EarI CTCTTC 7 cut(s) 99, 780, 1650, 2233, 2282, 3420, 3675
EciI GGCGGA 1 cut(s) 1815
Ecl136II GAGCTC 3 cut(s) 1492, 2095, 2243
Eco130I CCWWGG 4 cut(s) 481, 2397, 3054, 3213
Eco24I GRGCYC 3 cut(s) 1494, 2097, 2245
Eco31I GGTCTC 3 cut(s) 2754, 2916, 3638
Eco32I GATATC 1 cut(s) 3168
Eco47III AGCGCT 2 cut(s) 1444, 2623
Eco53kI GAGCTC 3 cut(s) 1492, 2095, 2243
Eco57I CTGAAG 2 cut(s) 1674, 1836
Eco72I CACGTG 1 cut(s) 2283
Eco81I CCTNAGG 1 cut(s) 595
Eco88I CYCGRG 2 cut(s) 1184, 2559
EcoICRI GAGCTC 3 cut(s) 1492, 2095, 2243
EcoRI GAATTC 2 cut(s) 533, 2689
EcoRII CCWGG 4 cut(s) 494, 1136, 2110, 2166
EcoRV GATATC 1 cut(s) 3168
EcoT14I CCWWGG 4 cut(s) 481, 2397, 3054, 3213
EcoT22I ATGCAT 3 cut(s) 1321, 1617, 3406
EcoT38I GRGCYC 3 cut(s) 1494, 2097, 2245
ErhI CCWWGG 4 cut(s) 481, 2397, 3054, 3213
Esp3I CGTCTC 1 cut(s) 347
FalI AAGNNNNNCTT 4 cut(s) 465, 497, 855, 887
FaqI GGGAC 4 cut(s) 1034, 1721, 2425, 3523
FauNDI CATATG 2 cut(s) 722, 3366
FbaI TGATCA 2 cut(s) 613, 3096
FblI GTMKAC 1 cut(s) 806
FokI GGATG 6 cut(s) 417, 495, 1973, 2786, 3482, 3734
FriOI GRGCYC 3 cut(s) 1494, 2097, 2245
FspBI CTAG 9 cut(s) 1038, 1868, 1940, 2058, 2138, 2189, 3014, 3422, 3818
GlaI GCGC 4 cut(s) 1444, 2623, 2827, 3395
GsaI CCCAGC 1 cut(s) 566
GsuI CTGGAG 4 cut(s) 281, 1159, 2246, 2873
HaeII RGCGCY 3 cut(s) 1446, 2625, 2829
HaeIII GGCC 7 cut(s) 44, 303, 1363, 1741, 2960, 3178, 3814
HapII CCGG 3 cut(s) 134, 2474, 3179
HgaI GACGC 2 cut(s) 224, 3520
HhaI GCGC 4 cut(s) 1445, 2624, 2828, 3396
Hin1I GRCGYC 1 cut(s) 2325
Hin6I GCGC 4 cut(s) 1443, 2622, 2826, 3394
HinP1I GCGC 4 cut(s) 1443, 2622, 2826, 3394
HincII GTYRAC 1 cut(s) 1046
HindII GTYRAC 1 cut(s) 1046
HindIII AAGCTT 1 cut(s) 3781
HpaII CCGG 3 cut(s) 134, 2474, 3179
HphI GGTGA 6 cut(s) 1066, 1406, 2100, 2906, 3719, 3932
Hpy166II GTNNAC 9 cut(s) 491, 807, 1046, 1388, 1945, 1988, 2366, 2897, 3308
Hpy8I GTNNAC 9 cut(s) 491, 807, 1046, 1388, 1945, 1988, 2366, 2897, 3308
Hpy99I CGWCG 2 cut(s) 1252, 2330
HpyAV CCTTC 7 cut(s) 116, 415, 435, 575, 1896, 2676, 3053
Hsp92I GRCGYC 1 cut(s) 2325
HspAI GCGC 4 cut(s) 1443, 2622, 2826, 3394
KpnI GGTACC 1 cut(s) 3359
Ksp22I TGATCA 2 cut(s) 613, 3096
LguI GCTCTTC 1 cut(s) 2233
LmnI GCTCC 9 cut(s) 520, 584, 596, 900, 1978, 2092, 2545, 3391, 3648
LweI GCATC 6 cut(s) 1123, 1179, 1261, 1285, 3460, 3833
MabI ACCWGGT 1 cut(s) 2110
MaeI CTAG 9 cut(s) 1038, 1868, 1940, 2058, 2138, 2189, 3014, 3422, 3818
MaeIII GTNAC 7 cut(s) 188, 236, 1054, 1770, 1890, 2009, 3325
MfeI CAATTG 1 cut(s) 2030
MflI RGATCY 7 cut(s) 208, 969, 1126, 1822, 2214, 2255, 3763
MhlI GDGCHC 6 cut(s) 1494, 1886, 2097, 2245, 2381, 2560
MlsI TGGCCA 2 cut(s) 303, 3814
MluNI TGGCCA 2 cut(s) 303, 3814
MlyI GAGTC 6 cut(s) 19, 545, 2299, 3118, 3180, 3503
MmeI TCCRAC 4 cut(s) 562, 1234, 2158, 2308
Mox20I TGGCCA 2 cut(s) 303, 3814
Mph1103I ATGCAT 3 cut(s) 1321, 1617, 3406
MroXI GAANNNNTTC 2 cut(s) 2463, 3784
MscI TGGCCA 2 cut(s) 303, 3814
MslI CAYNNNNRTG 3 cut(s) 1356, 2553, 3409
Msp20I TGGCCA 2 cut(s) 303, 3814
MspA1I CMGCKG 5 cut(s) 232, 562, 3293, 3701, 3742
MspCI CTTAAG 1 cut(s) 1859
MspI CCGG 3 cut(s) 134, 2474, 3179
MspR9I CCNGG 4 cut(s) 496, 1138, 2112, 2168
MunI CAATTG 1 cut(s) 2030
Mva1269I GAATGC 4 cut(s) 451, 1617, 2851, 3384
MvaI CCWGG 4 cut(s) 496, 1138, 2112, 2168
MvnI CGCG 1 cut(s) 3514
NcoI CCATGG 1 cut(s) 2397
NdeI CATATG 2 cut(s) 722, 3366
NlaIV GGNNCC 7 cut(s) 568, 586, 1128, 1824, 3177, 3357, 3874
NmeAIII GCCGAG 1 cut(s) 2224
NmuCI GTSAC 5 cut(s) 236, 1054, 1770, 2009, 3325
NsiI ATGCAT 3 cut(s) 1321, 1617, 3406
NspV TTCGAA 2 cut(s) 2018, 2680
PaeI GCATGC 2 cut(s) 396, 1323
PagI TCATGA 1 cut(s) 1992
PaqCI CACCTGC 1 cut(s) 631
PciI ACATGT 5 cut(s) 1011, 2151, 3019, 3322, 3729
PciSI GCTCTTC 1 cut(s) 2233
PcsI WCGNNNNNNNCGW 2 cut(s) 1886, 2279
PctI GAATGC 4 cut(s) 451, 1617, 2851, 3384
PdmI GAANNNNTTC 2 cut(s) 2463, 3784
PflMI CCANNNNNTGG 2 cut(s) 1115, 2403
PfoI TCCNGGA 1 cut(s) 2166
PinAI ACCGGT 1 cut(s) 2473
Ple19I CGATCG 1 cut(s) 2303
PleI GAGTC 6 cut(s) 19, 545, 2298, 3117, 3180, 3502
PmaCI CACGTG 1 cut(s) 2283
PmlI CACGTG 1 cut(s) 2283
PpsI GAGTC 6 cut(s) 19, 545, 2298, 3117, 3180, 3502
Ppu21I YACGTR 2 cut(s) 2283, 2555
PscI ACATGT 5 cut(s) 1011, 2151, 3019, 3322, 3729
PsiI TTATAA 1 cut(s) 3801
Psp124BI GAGCTC 3 cut(s) 1494, 2097, 2245
Psp1406I AACGTT 1 cut(s) 1370
Psp6I CCWGG 4 cut(s) 494, 1136, 2110, 2166
PspCI CACGTG 1 cut(s) 2283
PspFI CCCAGC 1 cut(s) 562
PspGI CCWGG 4 cut(s) 494, 1136, 2110, 2166
PspN4I GGNNCC 7 cut(s) 568, 586, 1128, 1824, 3177, 3357, 3874
PspPI GGNCC 3 cut(s) 42, 1361, 3176
PstI CTGCAG 1 cut(s) 1952
PstNI CAGNNNCTG 3 cut(s) 232, 1721, 3112
PsuI RGATCY 7 cut(s) 208, 969, 1126, 1822, 2214, 2255, 3763
PvuI CGATCG 1 cut(s) 2303
PvuII CAGCTG 4 cut(s) 232, 562, 3701, 3742
RsaI GTAC 9 cut(s) 1665, 1808, 1987, 2150, 2265, 2367, 2725, 3357, 3696
RsaNI GTAC 9 cut(s) 1664, 1807, 1986, 2149, 2264, 2366, 2724, 3356, 3695
RseI CAYNNNNRTG 3 cut(s) 1356, 2553, 3409
SacI GAGCTC 3 cut(s) 1494, 2097, 2245
SapI GCTCTTC 1 cut(s) 2233
Sau96I GGNCC 3 cut(s) 42, 1361, 3176
SchI GAGTC 6 cut(s) 19, 545, 2299, 3118, 3180, 3503
ScrFI CCNGG 4 cut(s) 496, 1138, 2112, 2168
SduI GDGCHC 6 cut(s) 1494, 1886, 2097, 2245, 2381, 2560
SexAI ACCWGGT 1 cut(s) 2110
SfaNI GCATC 6 cut(s) 1123, 1179, 1261, 1285, 3460, 3833
SfcI CTRYAG 4 cut(s) 375, 501, 1948, 2532
SfuI TTCGAA 2 cut(s) 2018, 2680
SmiMI CAYNNNNRTG 3 cut(s) 1356, 2553, 3409
SmlI CTYRAG 8 cut(s) 770, 899, 954, 1487, 1859, 2596, 2915, 3758
SmoI CTYRAG 8 cut(s) 770, 899, 954, 1487, 1859, 2596, 2915, 3758
SpeI ACTAGT 1 cut(s) 3817
SphI GCATGC 2 cut(s) 396, 1323
SsiI CCGC 7 cut(s) 10, 389, 726, 735, 1826, 2306, 3293
SspI AATATT 2 cut(s) 643, 881
SspMI CTAG 9 cut(s) 1038, 1868, 1940, 2058, 2138, 2189, 3014, 3422, 3818
SstI GAGCTC 3 cut(s) 1494, 2097, 2245
StyD4I CCNGG 4 cut(s) 494, 1136, 2110, 2166
StyI CCWWGG 4 cut(s) 481, 2397, 3054, 3213
TatI WGTACW 5 cut(s) 1663, 1806, 1985, 2148, 2365
TauI GCSGC 4 cut(s) 12, 392, 729, 738
TseFI GTSAC 5 cut(s) 236, 1054, 1770, 2009, 3325
Tsp45I GTSAC 5 cut(s) 236, 1054, 1770, 2009, 3325
TspDTI ATGAA 9 cut(s) 17, 434, 594, 1103, 2726, 3136, 3209, 3272, 3869
TspGWI ACGGA 3 cut(s) 1468, 2637, 3901
Van91I CCANNNNNTGG 2 cut(s) 1115, 2403
Vha464I CTTAAG 1 cut(s) 1859
XapI RAATTY 7 cut(s) 283, 533, 1284, 2050, 2689, 3437, 3900
XcmI CCANNNNNNNNNTGG 2 cut(s) 52, 1014
XmiI GTMKAC 1 cut(s) 806
XmnI GAANNNNTTC 2 cut(s) 2463, 3784
XspI CTAG 9 cut(s) 1038, 1868, 1940, 2058, 2138, 2189, 3014, 3422, 3818
ZraI GACGTC 1 cut(s) 2326
Zsp2I ATGCAT 3 cut(s) 1321, 1617, 3406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.