Rmu_sc0005149.1_g000009

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005149.1
OG0000203
Physical Location & Seq
Reverse (-)
52243 .. 58339
6097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005149.1_g000009.1.cds

Sequence Viewer

Length: 4503 bp
atggcagatattgtgattacagttgttgaaaaattagcagggtacacagtctcacctgttctgcgacagtttggttatctgattaactacaagagcaatgttcaagatcttaccgagaaggttacaactttgactgccaaaagagatggggtacaacgacgtgtagatgcagcgaagaggaactcagatgatattcttcctctagttgacgactggttggaacgagtaaagcagaccatagaagaaaaggagacatgttttgaaaaaggaagagtagccaaggcaacaacatgctgcagccccaatgggtggtgtccaaatctgaaggtccgtcattccttgagtaggaaagcaaagaagatggctccaaaggtagatgtcctacttacagaaggagatttcgggacaatatcatgtcctgctcgtgcgcagaagattgattttccagccatgcaatattttgaggattccactgaggcaccggcgaagccccatgaagaggctagctcctcctcaggaacatgcgattctgctaaaccaactgcacctatggatctctctgggcgtctgaaatataggttgccttatacaaaggatctcttgacggctttgcaagatgacaagatcagcatgattggcatttctgggacagtcagagagatagatacagtaacaactagagagttcatgaaaagaatgaaacaaaaaaagctctttgaagaggttgcgatggcagttgtgtcccaggatccagacttggaacgaatacaaggtgaaattgcagaaatgctggacctgaaattggagaatgggggtttggttgaacgggcagagaggctacgtgctgtattggcatccgcttctaagagcattcttgtaatactgactgatgtctatggaatacctgatttggaagcaattggaatttctcatgttgatatcaagaaaagcagcaagatcatgttgatttcacaaccatcagatgtgtttggtgaaatggggactcggagcaatttcaaattgttatcacaatccacttcccgaacaagacccgttgagttcgaatcaagaatttcagtcatcagagatgtaatggatgctctgacagatgatgaatccaaccccattgtaatctgtggcatggggggaattggcaaaacaacgttggtgatggaaattggtgcaaaagcgagcgaagttgatctatttgatgacgttgtaattgcagagtttactcaagaacccgacttgatcaaaatccaaggtaaaattgccaatgctctaggtctggaacttaaagcagaggatgacagagcagccaagttacgagaaagattatcagaggatacgaaaaaggtccttgtaatatttgacaatgtttggggacaactaaatctgtgggaactaggaattccaatttcccgggatccaaaaagttgcaaacttctggtatcatcacggaatcaagatacctttaaagagatgaaaaccaaaaagaacttcctcattggtagtttgctaaaacatgatgcctggagtttgtttaagaaggtagcaggatgttccattgaatccgattccgagctgcgtcctatagcagagcaggttctggatgcatgtgatggcttaccggttgcaatttctactgttggaagggcactacaagatgaaaaaattccagtatggagaaatgcacttcgacaactaagaatggcttgcccaaaagatgttccaggagttatggagcgtgtgtacggaaaaatagagttcagttatgaatgtctaccaagcaagcaagccaaatcatgttttttgctgtgttgcatatttccagaaagtgaagagtttcctattgaattgttggttatgtttgcgattgggcttggactttttaaagacattgattcaatatctgaagcaagggattacgtagcaacattggttgaaacactcaaatgtcgttctttattgttagacgacgatggggaagattgtgtgagaatgcatgatgtggtgcgtgacgtagccgtgtatatagcctccaaagagttagcacaaaagaagagtgataagtttgaaaggaagattatgccaataggaggagtggaattgaaccattggctcaagagcagttcattgacagccgatatgagtacttctgagcttttagtgttgggatccacagatggaattgaactggatgagctgccagctacaattttcaatggaacggaaaatctcaaggttttaatgatctcgaattcaatcctcacatccctttcacttccgaagaatcttcaaacactcattctatcggattgccgtctcaattttgatgtcattggagagctacggtcactcaagatactccagttttcaggtagatctgaactcaaacagttgcctgatacattcaaaaatttatctaatctgaggtcgttagatctgagaggatgtacagagcttacagcaatctcaccaggtgttctatcgagtttatctcgtctagaagaattgtacatgtgggatagctttgaagattgggtggttgagaaacaaggctcaacagaaacaatcaattggatctctcaattggaggttgatgatgaaccagaaggagaaccagaagaatctgaggttgatgcccaaccaatagacatcgacatatggaagacattcaacagattggcggttgagaaactagcctcaacagaaacaacaactgatctgatctctcgactggaggagggcgaggaagaggaggaggaggaggaggaggaggaggaggatgaggatgaggatgcagaaagtagcgaatcagaggaacgcgacagcgttcgagatctgttcgcagggtatgttcgagatttagaagaaaactacgaccatcgtacaaggtgggccaactctatggactggagagttggattactttcagagctactttccttgtcccgtttgactactttagaagttgttttaccaccaattgagattctgcgaaccagtaagctatttcataagcttgaaagatttaagatctctattggatgggatgagcatgaagacttggagtacttagagggtaactatttgagagttcatggtcttgatgcaagctctcttgcggatactggaatcagtttgttgttgaggaaaaccaacaagcttgagttaaaaatgaagaatttgagagaccctctcaatgtcttagatgtaaactgctatgcaaacttggagtcacttaccctcaacgactgtgattctctggaatatgtgattgacatgactggtaagccaaatgctccgcaaagtgtctttcctgtcttgaattatctggatatcgatggggcaagaaggctgaaagagatttttcatggtgacctgccactggggtcgcttcagaaactggatgcgctaactttatggaatctacctgtattgacgattatttggactagtggaagtcaatctagatggttgggagatctgccaactgtcgagtacatagacatacaggattgccaatcattggaagcaatttttgccttggaaggatcggaacatcatgtcaaagagatcaacttccgtagtttgataacgttgaaacttggaggtctaccaagcttcactggaatgagcaaaagtacttgtaaggggatggaaaggcaactggaagtcagcagtctccatcgcaatagtttgaccgttgaacattcacttttctctgaccccaaggttgtctttccagtcttggaagacctgaccattagccgcctgggcaacttgaaagagatatggaataaccaactttcacccaactccttctctgaattaagaacgctcactgtcgaggattgtgacaaactattgcatttggggccaacacaaatgcaaaatagattgcagagattggtgatcatacaggtattcaactgttcttcactggaagagattttcgaagtcggaagattgaccaccaatgaagggaatgcttcaatttctcagtcagacaaaataccctcaaatatttcccaacctgatcaagggatgcaaataaatgatatcattgatttcaaacaatcttgtcaaggctttcagaatttaattcatctaggagttcgatcttgtggcagtttgagataccttctcactccttcgattgccagaggttttgtcaagctcgaaagtctagagataggatcttgcgagaagatcgaagcaatagtcgcagcagatgagggtgaagaaacagaggatgaaggcatgctcccacagctctcttctttgacacttggggatctacccaatcttggaagcttttcgcaaggcaaatataattttgattggccactaatgcaaaagatcaatattaagaattgcaacaaaatgaacaacttttgttcaggatccctcagcatctcaagggaagtgagcatagacgtctctgattctggtgagaatcttgagcaggagctgaatgatagcaagaaagaaagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1500

Amino Acids

168.05

Weight (kDa)

4.77

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 4446
Acc16I TGCGCA 1 cut(s) 429
Acc36I ACCTGC 2 cut(s) 1594, 3393
AccB1I GGYRCC 1 cut(s) 478
AccB7I CCANNNNNTGG 2 cut(s) 309, 3532
AccI GTMKAC 2 cut(s) 1783, 3619
AccII CGCG 1 cut(s) 2829
AciI CCGC 5 cut(s) 858, 2690, 3128, 3308, 3775
AclI AACGTT 2 cut(s) 1163, 3602
AcoI YGGCCR 1 cut(s) 4349
AcsI RAATTY 8 cut(s) 924, 1071, 1410, 1674, 2260, 2419, 3187, 4102
AcuI CTGAAG 3 cut(s) 344, 1935, 3386
AcyI GRCGYC 2 cut(s) 565, 4443
AdeI CACNNNGTG 1 cut(s) 2483
AflIII ACRYGT 3 cut(s) 160, 254, 2520
AgeI ACCGGT 1 cut(s) 1630
AhlI ACTAGT 1 cut(s) 3458
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 5 cut(s) 744, 1532, 1732, 2479, 3777
AjuI GAANNNNNNNTTGG 4 cut(s) 800, 832, 1484, 1516
Alw26I GTCTC 6 cut(s) 55, 245, 2330, 3189, 3692, 4450
AlwNI CAGNNNCTG 3 cut(s) 1609, 3409, 4477
Ama87I CYCGRG 1 cut(s) 1422
AoxI GGCC 3 cut(s) 2901, 3881, 4349
ApeKI GCWGC 8 cut(s) 170, 294, 297, 951, 1316, 1585, 2206, 4232
ApoI RAATTY 8 cut(s) 924, 1071, 1410, 1674, 2260, 2419, 3187, 4102
ArsI GACNNNNNNTTYG 4 cut(s) 3224, 3256, 4212, 4244
AsiGI ACCGGT 1 cut(s) 1630
Asp700I GAANNNNTTC 4 cut(s) 1674, 1845, 2675, 4321
AspLEI GCGC 2 cut(s) 430, 3418
AspS9I GGNCC 5 cut(s) 328, 793, 1357, 2901, 3881
AsuC2I CCSGG 2 cut(s) 1423, 1424
AsuII TTCGAA 2 cut(s) 1062, 3960
AsuNHI GCTAGC 1 cut(s) 503
AvaI CYCGRG 1 cut(s) 1422
AvaII GGWCC 3 cut(s) 328, 793, 1357
AxyI CCTNAGG 1 cut(s) 514
BaeGI GKGCMC 1 cut(s) 1660
BaeI ACNNNNGTAYC 2 cut(s) 2357, 2390
BalI TGGCCA 1 cut(s) 4351
BamHI GGATCC 4 cut(s) 748, 1426, 2177, 4409
BanI GGYRCC 1 cut(s) 478
BarI GAAGNNNNNNTAC 2 cut(s) 2964, 2996
BauI CACGAG 1 cut(s) 423
BbsI GAAGAC 3 cut(s) 2678, 3072, 3765
BbvCI CCTCAGC 1 cut(s) 4415
BbvI GCAGC 8 cut(s) 182, 281, 309, 963, 1328, 1572, 2193, 4244
BceAI ACGGC 3 cut(s) 621, 2012, 2307
BciT130I CCWGG 5 cut(s) 746, 1534, 1734, 2481, 3779
BciVI GTATCC 2 cut(s) 1339, 3124
BclI TGATCA 3 cut(s) 1251, 3918, 4042
BcnI CCSGG 2 cut(s) 1423, 1424
BcoDI GTCTC 6 cut(s) 55, 245, 2330, 3189, 3692, 4450
BcuI ACTAGT 1 cut(s) 3458
BfmI CTRYAG 2 cut(s) 295, 1593
BfuAI ACCTGC 2 cut(s) 1594, 3393
BfuI GTATCC 2 cut(s) 1339, 3124
BglI GCCNNNNNGGC 1 cut(s) 3780
BglII AGATCT 6 cut(s) 106, 2384, 2443, 2842, 3039, 3487
BisI GCNGC 9 cut(s) 171, 295, 298, 952, 1317, 1586, 2207, 3775, 4233
BlsI GCNGC 9 cut(s) 172, 296, 299, 953, 1318, 1587, 2208, 3776, 4234
BmcAI AGTACT 3 cut(s) 2155, 3077, 3649
Bme1390I CCNGG 7 cut(s) 746, 1423, 1424, 1534, 1734, 2481, 3779
Bme18I GGWCC 3 cut(s) 328, 793, 1357
BmeT110I CYCGRG 1 cut(s) 1422
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 5 cut(s) 328, 793, 1357, 2901, 3881
BmiI GGNNCC 7 cut(s) 366, 480, 750, 1428, 2179, 3882, 4411
BmrFI CCNGG 7 cut(s) 746, 1423, 1424, 1534, 1734, 2481, 3779
BmrI ACTGGG 1 cut(s) 3401
BmtI GCTAGC 1 cut(s) 507
BmuI ACTGGG 1 cut(s) 3401
BoxI GACNNNNGTC 1 cut(s) 890
BpiI GAAGAC 3 cut(s) 2678, 3072, 3765
BplI GAGNNNNNCTC 8 cut(s) 491, 523, 2485, 2517, 3184, 3216, 3186, 3218
BpmI CTGGAG 4 cut(s) 1555, 2354, 2762, 2938
Bpu10I CCTNAGC 1 cut(s) 4415
Bpu14I TTCGAA 2 cut(s) 1062, 3960
BpuEI CTTGAG 8 cut(s) 361, 1221, 2108, 2225, 2345, 3191, 4408, 4487
BpuMI CCSGG 2 cut(s) 1423, 1424
Bsa29I ATCGAT 1 cut(s) 3345
BsaAI YACGTR 2 cut(s) 842, 1930
BsaBI GATNNNNATC 1 cut(s) 1256
BsaHI GRCGYC 2 cut(s) 565, 4443
BsaI GGTCTC 1 cut(s) 3189
BsaJI CCNNGG 7 cut(s) 279, 744, 1261, 1422, 3549, 3735, 3778
BsaWI WCCGGW 1 cut(s) 1630
BsaXI ACNNNNNCTCC 2 cut(s) 1736, 1766
Bse118I RCCGGY 2 cut(s) 481, 1630
Bse21I CCTNAGG 1 cut(s) 514
Bse3DI GCAATG 1 cut(s) 103
Bse8I GATNNNNATC 1 cut(s) 1256
BseBI CCWGG 5 cut(s) 746, 1534, 1734, 2481, 3779
BseCI ATCGAT 1 cut(s) 3345
BseDI CCNNGG 7 cut(s) 279, 744, 1261, 1422, 3549, 3735, 3778
BseJI GATNNNNATC 1 cut(s) 1256
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 9 cut(s) 198, 465, 528, 2151, 2423, 2438, 2625, 4019, 4429
BseSI GKGCMC 1 cut(s) 1660
BseXI GCAGC 8 cut(s) 182, 281, 309, 963, 1328, 1572, 2193, 4244
BsgI GTGCAG 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 2829
BshFI GGCC 3 cut(s) 2903, 3883, 4351
BshNI GGYRCC 1 cut(s) 478
BshTI ACCGGT 1 cut(s) 1630
BshVI ATCGAT 1 cut(s) 3345
BsiHKCI CYCGRG 1 cut(s) 1422
BsiSI CCGG 3 cut(s) 482, 1423, 1631
BslFI GGGAC 6 cut(s) 418, 661, 727, 1015, 1398, 2938
BsmAI GTCTC 6 cut(s) 55, 245, 2330, 3189, 3692, 4450
BsmBI CGTCTC 2 cut(s) 2330, 4450
BsmFI GGGAC 6 cut(s) 418, 661, 727, 1015, 1398, 2938
BsmI GAATGC 3 cut(s) 870, 2007, 3997
BsnI GGCC 3 cut(s) 2903, 3883, 4351
Bso31I GGTCTC 1 cut(s) 3189
BsoBI CYCGRG 1 cut(s) 1422
Bsp119I TTCGAA 2 cut(s) 1062, 3960
Bsp1286I GDGCHC 1 cut(s) 1660
Bsp1407I TGTACA 2 cut(s) 2456, 2517
BspACI CCGC 5 cut(s) 858, 2690, 3128, 3308, 3775
BspANI GGCC 3 cut(s) 2903, 3883, 4351
BspCNI CTCAG 9 cut(s) 197, 466, 527, 2152, 2424, 2439, 2626, 4018, 4428
BspDI ATCGAT 1 cut(s) 3345
BspFNI CGCG 1 cut(s) 2829
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 7 cut(s) 366, 480, 750, 1428, 2179, 3882, 4411
BspMAI CTGCAG 1 cut(s) 299
BspMI ACCTGC 2 cut(s) 1594, 3393
BspOI GCTAGC 1 cut(s) 507
BspT104I TTCGAA 2 cut(s) 1062, 3960
BspT107I GGYRCC 1 cut(s) 478
BspTNI GGTCTC 1 cut(s) 3189
BsrDI GCAATG 1 cut(s) 103
BsrFI RCCGGY 2 cut(s) 481, 1630
BsrGI TGTACA 2 cut(s) 2456, 2517
BssAI RCCGGY 2 cut(s) 481, 1630
BssECI CCNNGG 7 cut(s) 279, 744, 1261, 1422, 3549, 3735, 3778
BssNI GRCGYC 2 cut(s) 565, 4443
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 4 cut(s) 279, 1261, 3549, 3735
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 5 cut(s) 746, 1534, 1734, 2481, 3779
Bst6I CTCTTC 9 cut(s) 170, 265, 492, 714, 1836, 2057, 2751, 3945, 4288
BstACI GRCGYC 2 cut(s) 565, 4443
BstAPI GCANNNNNTGC 1 cut(s) 3545
BstAUI TGTACA 2 cut(s) 2456, 2517
BstBAI YACGTR 2 cut(s) 842, 1930
BstBI TTCGAA 2 cut(s) 1062, 3960
BstC8I GCNNGC 8 cut(s) 505, 1192, 1717, 1793, 1797, 2211, 3118, 4268
BstEII GGTNACC 1 cut(s) 3380
BstENI CCTNNNNNAGG 2 cut(s) 343, 4044
BstFNI CGCG 1 cut(s) 2829
BstHHI GCGC 2 cut(s) 430, 3418
BstMAI GTCTC 6 cut(s) 55, 245, 2330, 3189, 3692, 4450
BstMWI GCNNNNNNNGC 8 cut(s) 636, 851, 3545, 3780, 3880, 4229, 4276, 4357
BstNI CCWGG 5 cut(s) 746, 1534, 1734, 2481, 3779
BstNSI RCATGY 6 cut(s) 258, 294, 525, 1620, 2524, 4270
BstPAI GACNNNNGTC 1 cut(s) 890
BstPI GGTNACC 1 cut(s) 3380
BstSCI CCNGG 7 cut(s) 744, 1421, 1422, 1532, 1732, 2479, 3777
BstSFI CTRYAG 2 cut(s) 295, 1593
BstSLI GKGCMC 1 cut(s) 1660
BstSNI TACGTA 1 cut(s) 1930
BstUI CGCG 1 cut(s) 2829
BstV1I GCAGC 8 cut(s) 182, 281, 309, 963, 1328, 1572, 2193, 4244
BstV2I GAAGAC 3 cut(s) 2678, 3072, 3765
BstXI CCANNNNNNTGG 1 cut(s) 2911
Bsu15I ATCGAT 1 cut(s) 3345
Bsu36I CCTNAGG 1 cut(s) 514
BsuI GTATCC 2 cut(s) 1339, 3124
BsuRI GGCC 3 cut(s) 2903, 3883, 4351
BsuTUI ATCGAT 1 cut(s) 3345
BtgZI GCGATG 2 cut(s) 743, 3677
BtrI CACGTC 1 cut(s) 161
BtsIMutI CAGTG 5 cut(s) 471, 3389, 3630, 3846, 3944
BveI ACCTGC 2 cut(s) 1594, 3393
Cac8I GCNNGC 8 cut(s) 505, 1192, 1717, 1793, 1797, 2211, 3118, 4268
CaiI CAGNNNCTG 3 cut(s) 1609, 3409, 4477
CciI TCATGA 1 cut(s) 687
CfoI GCGC 2 cut(s) 430, 3418
Cfr10I RCCGGY 2 cut(s) 481, 1630
Cfr13I GGNCC 5 cut(s) 328, 793, 1357, 2901, 3881
Cfr9I CCCGGG 1 cut(s) 1422
ClaI ATCGAT 1 cut(s) 3345
CseI GACGC 2 cut(s) 554, 1577
CsiI ACCWGGT 1 cut(s) 2479
CspAI ACCGGT 1 cut(s) 1630
DraI TTTAAA 2 cut(s) 1477, 1894
DraIII CACNNNGTG 1 cut(s) 2483
EaeI YGGCCR 1 cut(s) 4349
Eam1104I CTCTTC 9 cut(s) 170, 265, 492, 714, 1836, 2057, 2751, 3945, 4288
EarI CTCTTC 9 cut(s) 170, 265, 492, 714, 1836, 2057, 2751, 3945, 4288
Eco105I TACGTA 1 cut(s) 1930
Eco130I CCWWGG 4 cut(s) 279, 1261, 3549, 3735
Eco31I GGTCTC 1 cut(s) 3189
Eco32I GATATC 3 cut(s) 940, 3343, 4066
Eco47I GGWCC 3 cut(s) 328, 793, 1357
Eco57I CTGAAG 3 cut(s) 344, 1935, 3386
Eco81I CCTNAGG 1 cut(s) 514
Eco88I CYCGRG 1 cut(s) 1422
Eco91I GGTNACC 1 cut(s) 3380
EcoNI CCTNNNNNAGG 2 cut(s) 343, 4044
EcoO109I RGGNCCY 1 cut(s) 1357
EcoO65I GGTNACC 1 cut(s) 3380
EcoRI GAATTC 2 cut(s) 1410, 2260
EcoRII CCWGG 5 cut(s) 744, 1532, 1732, 2479, 3777
EcoRV GATATC 3 cut(s) 940, 3343, 4066
EcoT14I CCWWGG 4 cut(s) 279, 1261, 3549, 3735
EcoT22I ATGCAT 2 cut(s) 1618, 2007
ErhI CCWWGG 4 cut(s) 279, 1261, 3549, 3735
Esp3I CGTCTC 2 cut(s) 2330, 4450
FalI AAGNNNNNCTT 6 cut(s) 584, 616, 1699, 1731, 3728, 3760
FaqI GGGAC 6 cut(s) 418, 661, 727, 1015, 1398, 2938
FauNDI CATATG 1 cut(s) 2666
FbaI TGATCA 3 cut(s) 1251, 3918, 4042
FblI GTMKAC 2 cut(s) 1783, 3619
Fnu4HI GCNGC 9 cut(s) 171, 295, 298, 952, 1317, 1586, 2207, 3775, 4233
Fsp4HI GCNGC 9 cut(s) 171, 295, 298, 952, 1317, 1586, 2207, 3775, 4233
FspI TGCGCA 1 cut(s) 429
GlaI GCGC 2 cut(s) 429, 3417
GluI GCNGC 9 cut(s) 171, 295, 298, 952, 1317, 1586, 2207, 3775, 4233
GsuI CTGGAG 4 cut(s) 1555, 2354, 2762, 2938
HaeIII GGCC 3 cut(s) 2903, 3883, 4351
HapII CCGG 3 cut(s) 482, 1423, 1631
HgaI GACGC 2 cut(s) 554, 1577
HhaI GCGC 2 cut(s) 430, 3418
Hin1I GRCGYC 2 cut(s) 565, 4443
Hin6I GCGC 2 cut(s) 428, 3416
HinP1I GCGC 2 cut(s) 428, 3416
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 4 cut(s) 3023, 3167, 3625, 4318
HpaII CCGG 3 cut(s) 482, 1423, 1631
Hpy166II GTNNAC 7 cut(s) 45, 208, 1233, 1753, 1784, 3220, 3620
Hpy8I GTNNAC 7 cut(s) 45, 208, 1233, 1753, 1784, 3220, 3620
Hpy99I CGWCG 2 cut(s) 162, 1982
HpyCH4IV ACGT 8 cut(s) 160, 841, 1163, 1215, 1929, 2022, 3602, 4443
HpyF10VI GCNNNNNNNGC 8 cut(s) 636, 851, 3545, 3780, 3880, 4229, 4276, 4357
HpySE526I ACGT 8 cut(s) 160, 841, 1163, 1215, 1929, 2022, 3602, 4443
Hsp92I GRCGYC 2 cut(s) 565, 4443
HspAI GCGC 2 cut(s) 428, 3416
Ksp22I TGATCA 3 cut(s) 1251, 3918, 4042
LmnI GCTCC 7 cut(s) 370, 512, 1008, 1744, 3310, 4275, 4474
Lsp1109I GCAGC 8 cut(s) 182, 281, 309, 963, 1328, 1572, 2193, 4244
MabI ACCWGGT 1 cut(s) 2479
MaeII ACGT 8 cut(s) 160, 841, 1163, 1215, 1929, 2022, 3602, 4443
MaeIII GTNAC 9 cut(s) 121, 670, 1323, 2018, 2355, 3086, 3240, 3380, 3860
MfeI CAATTG 4 cut(s) 918, 2578, 2591, 2988
MhlI GDGCHC 1 cut(s) 1660
MlsI TGGCCA 1 cut(s) 4351
MluNI TGGCCA 1 cut(s) 4351
MlyI GAGTC 2 cut(s) 997, 3248
MmeI TCCRAC 5 cut(s) 198, 1143, 1630, 2905, 3946
Mox20I TGGCCA 1 cut(s) 4351
Mph1103I ATGCAT 2 cut(s) 1618, 2007
MroXI GAANNNNTTC 4 cut(s) 1674, 1845, 2675, 4321
MscI TGGCCA 1 cut(s) 4351
MslI CAYNNNNRTG 2 cut(s) 1954, 3635
Msp20I TGGCCA 1 cut(s) 4351
MspI CCGG 3 cut(s) 482, 1423, 1631
MspR9I CCNGG 7 cut(s) 746, 1423, 1424, 1534, 1734, 2481, 3779
MunI CAATTG 4 cut(s) 918, 2578, 2591, 2988
Mva1269I GAATGC 3 cut(s) 870, 2007, 3997
MvaI CCWGG 5 cut(s) 746, 1534, 1734, 2481, 3779
MvnI CGCG 1 cut(s) 2829
MwoI GCNNNNNNNGC 8 cut(s) 636, 851, 3545, 3780, 3880, 4229, 4276, 4357
NciI CCSGG 2 cut(s) 1423, 1424
NdeI CATATG 1 cut(s) 2666
NheI GCTAGC 1 cut(s) 503
NlaIV GGNNCC 7 cut(s) 366, 480, 750, 1428, 2179, 3882, 4411
NmuCI GTSAC 5 cut(s) 2018, 2355, 3240, 3380, 3860
NsbI TGCGCA 1 cut(s) 429
NsiI ATGCAT 2 cut(s) 1618, 2007
NspI RCATGY 6 cut(s) 258, 294, 525, 1620, 2524, 4270
NspV TTCGAA 2 cut(s) 1062, 3960
PaeI GCATGC 1 cut(s) 4270
PagI TCATGA 1 cut(s) 687
PciI ACATGT 2 cut(s) 254, 2520
PctI GAATGC 3 cut(s) 870, 2007, 3997
PdmI GAANNNNTTC 4 cut(s) 1674, 1845, 2675, 4321
PflMI CCANNNNNTGG 2 cut(s) 309, 3532
PfoI TCCNGGA 1 cut(s) 1732
PinAI ACCGGT 1 cut(s) 1630
PkrI GCNGC 9 cut(s) 172, 296, 299, 953, 1318, 1587, 2208, 3776, 4234
PleI GAGTC 2 cut(s) 997, 3247
PpsI GAGTC 2 cut(s) 997, 3247
Ppu21I YACGTR 2 cut(s) 842, 1930
PpuMI RGGWCCY 1 cut(s) 1357
PscI ACATGT 2 cut(s) 254, 2520
PshAI GACNNNNGTC 1 cut(s) 890
Psp1406I AACGTT 2 cut(s) 1163, 3602
Psp5II RGGWCCY 1 cut(s) 1357
Psp6I CCWGG 5 cut(s) 744, 1532, 1732, 2479, 3777
PspEI GGTNACC 1 cut(s) 3380
PspGI CCWGG 5 cut(s) 744, 1532, 1732, 2479, 3777
PspN4I GGNNCC 7 cut(s) 366, 480, 750, 1428, 2179, 3882, 4411
PspPI GGNCC 5 cut(s) 328, 793, 1357, 2901, 3881
PspPPI RGGWCCY 1 cut(s) 1357
PstI CTGCAG 1 cut(s) 299
PstNI CAGNNNCTG 3 cut(s) 1609, 3409, 4477
RseI CAYNNNNRTG 2 cut(s) 1954, 3635
SatI GCNGC 9 cut(s) 171, 295, 298, 952, 1317, 1586, 2207, 3775, 4233
Sau96I GGNCC 5 cut(s) 328, 793, 1357, 2901, 3881
ScaI AGTACT 3 cut(s) 2155, 3077, 3649
SchI GAGTC 2 cut(s) 997, 3248
ScrFI CCNGG 7 cut(s) 746, 1423, 1424, 1534, 1734, 2481, 3779
SduI GDGCHC 1 cut(s) 1660
SexAI ACCWGGT 1 cut(s) 2479
SfcI CTRYAG 2 cut(s) 295, 1593
SfuI TTCGAA 2 cut(s) 1062, 3960
SgrAI CRCCGGYG 1 cut(s) 481
SinI GGWCC 3 cut(s) 328, 793, 1357
SmaI CCCGGG 1 cut(s) 1424
SmiMI CAYNNNNRTG 2 cut(s) 1954, 3635
SmlI CTYRAG 8 cut(s) 340, 1236, 2123, 2240, 2360, 3170, 4423, 4466
SmoI CTYRAG 8 cut(s) 340, 1236, 2123, 2240, 2360, 3170, 4423, 4466
SnaBI TACGTA 1 cut(s) 1930
SpeI ACTAGT 1 cut(s) 3458
SphI GCATGC 1 cut(s) 4270
SsiI CCGC 5 cut(s) 858, 2690, 3128, 3308, 3775
SspI AATATT 4 cut(s) 458, 1368, 4030, 4372
StyD4I CCNGG 7 cut(s) 744, 1421, 1422, 1532, 1732, 2479, 3777
StyI CCWWGG 4 cut(s) 279, 1261, 3549, 3735
TaiI ACGT 8 cut(s) 163, 844, 1166, 1218, 1932, 2025, 3605, 4446
TatI WGTACW 6 cut(s) 2153, 2456, 2517, 3075, 3504, 3647
TauI GCSGC 1 cut(s) 3777
TscAI CASTG 5 cut(s) 478, 3396, 3637, 3853, 3951
TseFI GTSAC 5 cut(s) 2018, 2355, 3240, 3380, 3860
TseI GCWGC 8 cut(s) 170, 294, 297, 951, 1316, 1585, 2206, 4232
Tsp45I GTSAC 5 cut(s) 2018, 2355, 3240, 3380, 3860
TspGWI ACGGA 5 cut(s) 320, 1474, 1770, 2246, 3578
TspMI CCCGGG 1 cut(s) 1422
TspRI CASTG 5 cut(s) 478, 3396, 3637, 3853, 3951
Van91I CCANNNNNTGG 2 cut(s) 309, 3532
VpaK11BI GGWCC 3 cut(s) 328, 793, 1357
XagI CCTNNNNNAGG 2 cut(s) 343, 4044
XapI RAATTY 8 cut(s) 924, 1071, 1410, 1674, 2260, 2419, 3187, 4102
XbaI TCTAGA 3 cut(s) 2506, 3473, 4192
XceI RCATGY 6 cut(s) 258, 294, 525, 1620, 2524, 4270
XcmI CCANNNNNNNNNTGG 1 cut(s) 3775
XmaI CCCGGG 1 cut(s) 1422
XmiI GTMKAC 2 cut(s) 1783, 3619
XmnI GAANNNNTTC 4 cut(s) 1674, 1845, 2675, 4321
ZraI GACGTC 1 cut(s) 4444
ZrmI AGTACT 3 cut(s) 2155, 3077, 3649
Zsp2I ATGCAT 2 cut(s) 1618, 2007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.