ADP binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 947 |
| AccIII | TCCGGA | 1 cut(s) | 636 |
| AciI | CCGC | 2 cut(s) | 156, 674 |
| AclWI | GGATC | 2 cut(s) | 73, 538 |
| AcsI | RAATTY | 3 cut(s) | 178, 352, 787 |
| AcuI | CTGAAG | 1 cut(s) | 219 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 181, 425 |
| AgeI | ACCGGT | 1 cut(s) | 110 |
| AgsI | TTSAA | 2 cut(s) | 313, 602 |
| AjnI | CCWGG | 1 cut(s) | 209 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 338, 370 |
| AluBI | AGCT | 4 cut(s) | 20, 456, 554, 784 |
| AluI | AGCT | 4 cut(s) | 20, 456, 554, 784 |
| Alw26I | GTCTC | 5 cut(s) | 135, 179, 200, 242, 940 |
| AlwI | GGATC | 2 cut(s) | 73, 538 |
| Aor13HI | TCCGGA | 1 cut(s) | 636 |
| ApeKI | GCWGC | 5 cut(s) | 167, 170, 453, 488, 491 |
| ApoI | RAATTY | 3 cut(s) | 178, 352, 787 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 299, 331 |
| AsiGI | ACCGGT | 1 cut(s) | 110 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 629 |
| AspS9I | GGNCC | 1 cut(s) | 512 |
| AsuC2I | CCSGG | 2 cut(s) | 175, 419 |
| AsuHPI | GGTGA | 4 cut(s) | 100, 160, 344, 896 |
| AsuII | TTCGAA | 1 cut(s) | 305 |
| AvaII | GGWCC | 1 cut(s) | 512 |
| AxyI | CCTNAGG | 1 cut(s) | 460 |
| BauI | CACGAG | 1 cut(s) | 391 |
| BbvCI | CCTCAGC | 1 cut(s) | 516 |
| BbvI | GCAGC | 5 cut(s) | 179, 182, 465, 500, 503 |
| BccI | CCATC | 2 cut(s) | 374, 959 |
| BceAI | ACGGC | 1 cut(s) | 143 |
| BciT130I | CCWGG | 1 cut(s) | 211 |
| BciVI | GTATCC | 2 cut(s) | 414, 705 |
| BcnI | CCSGG | 2 cut(s) | 175, 419 |
| BcoDI | GTCTC | 5 cut(s) | 135, 179, 200, 242, 940 |
| BfaI | CTAG | 2 cut(s) | 792, 977 |
| BfuI | GTATCC | 2 cut(s) | 414, 705 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 164 |
| BisI | GCNGC | 5 cut(s) | 168, 171, 454, 489, 492 |
| BlsI | GCNGC | 5 cut(s) | 169, 172, 455, 490, 493 |
| Bme1390I | CCNGG | 3 cut(s) | 175, 211, 419 |
| Bme18I | GGWCC | 1 cut(s) | 512 |
| BmgT120I | GGNCC | 1 cut(s) | 512 |
| BmiI | GGNNCC | 1 cut(s) | 513 |
| BmrFI | CCNGG | 3 cut(s) | 175, 211, 419 |
| BmsI | GCATC | 4 cut(s) | 112, 601, 890, 971 |
| BpmI | CTGGAG | 2 cut(s) | 468, 922 |
| Bpu10I | CCTNAGC | 1 cut(s) | 516 |
| Bpu14I | TTCGAA | 1 cut(s) | 305 |
| BpuEI | CTTGAG | 1 cut(s) | 788 |
| BpuMI | CCSGG | 2 cut(s) | 175, 419 |
| Bsa29I | ATCGAT | 1 cut(s) | 573 |
| BsaI | GGTCTC | 1 cut(s) | 200 |
| BsaJI | CCNNGG | 5 cut(s) | 159, 281, 417, 804, 877 |
| BsaWI | WCCGGW | 3 cut(s) | 52, 110, 636 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 34, 64, 178, 208, 413, 443 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 181, 425 |
| Bse118I | RCCGGY | 1 cut(s) | 110 |
| Bse1I | ACTGG | 2 cut(s) | 36, 939 |
| Bse21I | CCTNAGG | 1 cut(s) | 460 |
| Bse3DI | GCAATG | 2 cut(s) | 226, 501 |
| BseAI | TCCGGA | 1 cut(s) | 636 |
| BseBI | CCWGG | 1 cut(s) | 211 |
| BseCI | ATCGAT | 1 cut(s) | 573 |
| BseDI | CCNNGG | 5 cut(s) | 159, 281, 417, 804, 877 |
| BseGI | GGATG | 3 cut(s) | 633, 925, 951 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 181, 425 |
| BseMI | GCAATG | 2 cut(s) | 226, 501 |
| BseMII | CTCAG | 4 cut(s) | 50, 111, 474, 530 |
| BseNI | ACTGG | 2 cut(s) | 36, 939 |
| BseRI | GAGGAG | 4 cut(s) | 402, 448, 471, 554 |
| BseXI | GCAGC | 5 cut(s) | 179, 182, 465, 500, 503 |
| BseYI | CCCAGC | 1 cut(s) | 736 |
| BshTI | ACCGGT | 1 cut(s) | 110 |
| BshVI | ATCGAT | 1 cut(s) | 573 |
| BsiSI | CCGG | 5 cut(s) | 53, 111, 175, 419, 637 |
| BslFI | GGGAC | 1 cut(s) | 673 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 181, 425 |
| BsmAI | GTCTC | 5 cut(s) | 135, 179, 200, 242, 940 |
| BsmBI | CGTCTC | 1 cut(s) | 135 |
| BsmFI | GGGAC | 1 cut(s) | 673 |
| Bso31I | GGTCTC | 1 cut(s) | 200 |
| Bsp119I | TTCGAA | 1 cut(s) | 305 |
| Bsp13I | TCCGGA | 1 cut(s) | 636 |
| Bsp143I | GATC | 3 cut(s) | 78, 543, 906 |
| Bsp19I | CCATGG | 1 cut(s) | 804 |
| BspACI | CCGC | 2 cut(s) | 156, 674 |
| BspCNI | CTCAG | 4 cut(s) | 49, 112, 473, 529 |
| BspDI | ATCGAT | 1 cut(s) | 573 |
| BspEI | TCCGGA | 1 cut(s) | 636 |
| BspLI | GGNNCC | 1 cut(s) | 513 |
| BspPI | GGATC | 2 cut(s) | 73, 538 |
| BspT104I | TTCGAA | 1 cut(s) | 305 |
| BspTNI | GGTCTC | 1 cut(s) | 200 |
| BsrDI | GCAATG | 2 cut(s) | 226, 501 |
| BsrFI | RCCGGY | 1 cut(s) | 110 |
| BsrI | ACTGG | 2 cut(s) | 36, 939 |
| BssAI | RCCGGY | 1 cut(s) | 110 |
| BssECI | CCNNGG | 5 cut(s) | 159, 281, 417, 804, 877 |
| BssMI | GATC | 3 cut(s) | 78, 543, 906 |
| BssNAI | GTATAC | 1 cut(s) | 948 |
| BssSI | CACGAG | 1 cut(s) | 391 |
| BssT1I | CCWWGG | 3 cut(s) | 281, 804, 877 |
| Bst1107I | GTATAC | 1 cut(s) | 948 |
| Bst2BI | CACGAG | 1 cut(s) | 391 |
| Bst2UI | CCWGG | 1 cut(s) | 211 |
| Bst4CI | ACNGT | 1 cut(s) | 46 |
| Bst6I | CTCTTC | 2 cut(s) | 660, 1001 |
| BstBI | TTCGAA | 1 cut(s) | 305 |
| BstC8I | GCNNGC | 1 cut(s) | 774 |
| BstDEI | CTNAG | 6 cut(s) | 36, 120, 342, 374, 460, 516 |
| BstDSI | CCRYGG | 2 cut(s) | 159, 804 |
| BstF5I | GGATG | 3 cut(s) | 633, 925, 951 |
| BstKTI | GATC | 3 cut(s) | 81, 546, 909 |
| BstMAI | GTCTC | 5 cut(s) | 135, 179, 200, 242, 940 |
| BstMBI | GATC | 3 cut(s) | 78, 543, 906 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 164, 485 |
| BstNI | CCWGG | 1 cut(s) | 211 |
| BstSCI | CCNGG | 3 cut(s) | 173, 209, 417 |
| BstV1I | GCAGC | 5 cut(s) | 179, 182, 465, 500, 503 |
| BstX2I | RGATCY | 1 cut(s) | 543 |
| BstYI | RGATCY | 1 cut(s) | 543 |
| BstZ17I | GTATAC | 1 cut(s) | 948 |
| Bsu15I | ATCGAT | 1 cut(s) | 573 |
| Bsu36I | CCTNAGG | 1 cut(s) | 460 |
| BsuI | GTATCC | 2 cut(s) | 414, 705 |
| BsuTUI | ATCGAT | 1 cut(s) | 573 |
| BtgI | CCRYGG | 2 cut(s) | 159, 804 |
| BtsCI | GGATG | 3 cut(s) | 633, 925, 951 |
| BtsIMutI | CAGTG | 2 cut(s) | 42, 946 |
| Cac8I | GCNNGC | 1 cut(s) | 774 |
| Cfr10I | RCCGGY | 1 cut(s) | 110 |
| Cfr13I | GGNCC | 1 cut(s) | 512 |
| ClaI | ATCGAT | 1 cut(s) | 573 |
| CspAI | ACCGGT | 1 cut(s) | 110 |
| CviAII | CATG | 3 cut(s) | 443, 799, 805 |
| DdeI | CTNAG | 6 cut(s) | 36, 120, 342, 374, 460, 516 |
| DpnI | GATC | 3 cut(s) | 80, 545, 908 |
| DpnII | GATC | 3 cut(s) | 78, 543, 906 |
| Eam1104I | CTCTTC | 2 cut(s) | 660, 1001 |
| EarI | CTCTTC | 2 cut(s) | 660, 1001 |
| Eco130I | CCWWGG | 3 cut(s) | 281, 804, 877 |
| Eco31I | GGTCTC | 1 cut(s) | 200 |
| Eco47I | GGWCC | 1 cut(s) | 512 |
| Eco57I | CTGAAG | 1 cut(s) | 219 |
| Eco81I | CCTNAGG | 1 cut(s) | 460 |
| EcoO109I | RGGNCCY | 1 cut(s) | 512 |
| EcoRII | CCWGG | 1 cut(s) | 209 |
| EcoT14I | CCWWGG | 3 cut(s) | 281, 804, 877 |
| ErhI | CCWWGG | 3 cut(s) | 281, 804, 877 |
| Esp3I | CGTCTC | 1 cut(s) | 135 |
| FaeI | CATG | 3 cut(s) | 446, 802, 808 |
| FaqI | GGGAC | 1 cut(s) | 673 |
| FatI | CATG | 3 cut(s) | 442, 798, 804 |
| FauI | CCCGC | 1 cut(s) | 163 |
| FblI | GTMKAC | 1 cut(s) | 947 |
| Fnu4HI | GCNGC | 5 cut(s) | 168, 171, 454, 489, 492 |
| FokI | GGATG | 3 cut(s) | 620, 912, 938 |
| Fsp4HI | GCNGC | 5 cut(s) | 168, 171, 454, 489, 492 |
| FspBI | CTAG | 2 cut(s) | 792, 977 |
| GluI | GCNGC | 5 cut(s) | 168, 171, 454, 489, 492 |
| GsaI | CCCAGC | 1 cut(s) | 740 |
| GsuI | CTGGAG | 2 cut(s) | 468, 922 |
| HapII | CCGG | 5 cut(s) | 53, 111, 175, 419, 637 |
| Hin1II | CATG | 3 cut(s) | 446, 802, 808 |
| HincII | GTYRAC | 1 cut(s) | 364 |
| HindII | GTYRAC | 1 cut(s) | 364 |
| HinfI | GANTC | 6 cut(s) | 49, 642, 694, 795, 809, 873 |
| HpaII | CCGG | 5 cut(s) | 53, 111, 175, 419, 637 |
| HphI | GGTGA | 4 cut(s) | 100, 160, 344, 896 |
| Hpy166II | GTNNAC | 5 cut(s) | 42, 226, 364, 764, 948 |
| Hpy188I | TCNGA | 4 cut(s) | 472, 538, 930, 967 |
| Hpy188III | TCNNGA | 8 cut(s) | 197, 391, 462, 547, 637, 698, 792, 826 |
| Hpy8I | GTNNAC | 5 cut(s) | 42, 226, 364, 764, 948 |
| Hpy99I | CGWCG | 1 cut(s) | 827 |
| HpyAV | CCTTC | 3 cut(s) | 331, 380, 440 |
| HpyCH4III | ACNGT | 1 cut(s) | 46 |
| HpyCH4V | TGCA | 4 cut(s) | 12, 219, 559, 903 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 164, 485 |
| HpyF3I | CTNAG | 6 cut(s) | 36, 120, 342, 374, 460, 516 |
| Hsp92II | CATG | 3 cut(s) | 446, 802, 808 |
| Kpn2I | TCCGGA | 1 cut(s) | 636 |
| Kzo9I | GATC | 3 cut(s) | 78, 543, 906 |
| LmnI | GCTCC | 4 cut(s) | 199, 415, 461, 484 |
| Lsp1109I | GCAGC | 5 cut(s) | 179, 182, 465, 500, 503 |
| LweI | GCATC | 4 cut(s) | 112, 601, 890, 971 |
| MaeI | CTAG | 2 cut(s) | 792, 977 |
| MaeIII | GTNAC | 3 cut(s) | 22, 46, 616 |
| MalI | GATC | 3 cut(s) | 80, 545, 908 |
| MboI | GATC | 3 cut(s) | 78, 543, 906 |
| MboII | GAAGA | 4 cut(s) | 358, 620, 677, 764 |
| MflI | RGATCY | 1 cut(s) | 543 |
| MluCI | AATT | 6 cut(s) | 178, 352, 597, 787, 848, 985 |
| MlyI | GAGTC | 3 cut(s) | 43, 651, 803 |
| MmeI | TCCRAC | 1 cut(s) | 850 |
| MroI | TCCGGA | 1 cut(s) | 636 |
| MroXI | GAANNNNTTC | 1 cut(s) | 629 |
| MseI | TTAA | 2 cut(s) | 68, 291 |
| MslI | CAYNNNNRTG | 2 cut(s) | 803, 930 |
| MspA1I | CMGCKG | 1 cut(s) | 20 |
| MspI | CCGG | 5 cut(s) | 53, 111, 175, 419, 637 |
| MspR9I | CCNGG | 3 cut(s) | 175, 211, 419 |
| MvaI | CCWGG | 1 cut(s) | 211 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 164, 485 |
| NciI | CCSGG | 2 cut(s) | 175, 419 |
| NcoI | CCATGG | 1 cut(s) | 804 |
| NdeII | GATC | 3 cut(s) | 78, 543, 906 |
| NlaIII | CATG | 3 cut(s) | 446, 802, 808 |
| NlaIV | GGNNCC | 1 cut(s) | 513 |
| NmuCI | GTSAC | 1 cut(s) | 46 |
| NspV | TTCGAA | 1 cut(s) | 305 |
| PdmI | GAANNNNTTC | 1 cut(s) | 629 |
| PfeI | GAWTC | 3 cut(s) | 694, 795, 873 |
| PinAI | ACCGGT | 1 cut(s) | 110 |
| PkrI | GCNGC | 5 cut(s) | 169, 172, 455, 490, 493 |
| PleI | GAGTC | 3 cut(s) | 43, 650, 803 |
| PpsI | GAGTC | 3 cut(s) | 43, 650, 803 |
| PpuMI | RGGWCCY | 1 cut(s) | 512 |
| Psp5II | RGGWCCY | 1 cut(s) | 512 |
| Psp6I | CCWGG | 1 cut(s) | 209 |
| PspFI | CCCAGC | 1 cut(s) | 736 |
| PspGI | CCWGG | 1 cut(s) | 209 |
| PspN4I | GGNNCC | 1 cut(s) | 513 |
| PspPI | GGNCC | 1 cut(s) | 512 |
| PspPPI | RGGWCCY | 1 cut(s) | 512 |
| PsuI | RGATCY | 1 cut(s) | 543 |
| PvuII | CAGCTG | 1 cut(s) | 20 |
| RseI | CAYNNNNRTG | 2 cut(s) | 803, 930 |
| SaqAI | TTAA | 2 cut(s) | 68, 291 |
| SatI | GCNGC | 5 cut(s) | 168, 171, 454, 489, 492 |
| Sau3AI | GATC | 3 cut(s) | 78, 543, 906 |
| Sau96I | GGNCC | 1 cut(s) | 512 |
| SchI | GAGTC | 3 cut(s) | 43, 651, 803 |
| ScrFI | CCNGG | 3 cut(s) | 175, 211, 419 |
| SfaNI | GCATC | 4 cut(s) | 112, 601, 890, 971 |
| SfuI | TTCGAA | 1 cut(s) | 305 |
| SinI | GGWCC | 1 cut(s) | 512 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 803, 930 |
| SmlI | CTYRAG | 1 cut(s) | 767 |
| SmoI | CTYRAG | 1 cut(s) | 767 |
| Sse9I | AATT | 6 cut(s) | 178, 352, 597, 787, 848, 985 |
| SsiI | CCGC | 2 cut(s) | 156, 674 |
| SspMI | CTAG | 2 cut(s) | 792, 977 |
| StyD4I | CCNGG | 3 cut(s) | 173, 209, 417 |
| StyI | CCWWGG | 3 cut(s) | 281, 804, 877 |
| TaaI | ACNGT | 1 cut(s) | 46 |
| TaqI | TCGA | 3 cut(s) | 305, 573, 812 |
| TasI | AATT | 6 cut(s) | 178, 352, 597, 787, 848, 985 |
| TfiI | GAWTC | 3 cut(s) | 694, 795, 873 |
| Tru1I | TTAA | 2 cut(s) | 68, 291 |
| Tru9I | TTAA | 2 cut(s) | 68, 291 |
| TscAI | CASTG | 2 cut(s) | 49, 946 |
| TseFI | GTSAC | 1 cut(s) | 46 |
| TseI | GCWGC | 5 cut(s) | 167, 170, 453, 488, 491 |
| Tsp45I | GTSAC | 1 cut(s) | 46 |
| TspDTI | ATGAA | 4 cut(s) | 365, 459, 622, 678 |
| TspRI | CASTG | 2 cut(s) | 49, 946 |
| VpaK11BI | GGWCC | 1 cut(s) | 512 |
| XapI | RAATTY | 3 cut(s) | 178, 352, 787 |
| XbaI | TCTAGA | 1 cut(s) | 791 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 241 |
| XmiI | GTMKAC | 1 cut(s) | 947 |
| XmnI | GAANNNNTTC | 1 cut(s) | 629 |
| XspI | CTAG | 2 cut(s) | 792, 977 |