Rh6CG253000

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000203
Physical Location & Seq
Forward (+)
42319938 .. 42325109
5172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG253000.1

Sequence Viewer

Length: 3642 bp
ATGGAAGCTATCGTTTCATCAGTTCTTGGAAAACTAGCAGAGTACACAGTTGAACCGGTTCTGCGACAGTTTGGTTATCTGATTCACTATGAGAGTAACGTTGCTCGTCTCAACAACCAAGTTGAGAGGCTGAATGCCCAAAGACGCGACGTGGCAGAAAAAATTGAAGCAGCGACCAGGAATTCAGAGACTATTCTTCACCAAGTTGAGATCTGGTTGGACCAGGTGGATCGAACCTTACGAGACAAGGATACATGTTTTGAAAACGAGCGAACAGCAAAGGCGGAATGCTGCTCAAATGGCTGGTTCCCCAATTTGAAGATACGGCATTCCTTGAGTAGGAAAGCGAAGAAGATGACTCCGGATGTCGATAGCCTCATTGATAACAAGTTTGAGGTTGTAGGCTTCGCTGCCAGATATGAAGGGGCCAGCTCCTCCCAGGCATCCAACATTATGAATTTTGAATCAAGAAAATCAACCATTGAAGGTGTGATGGAAGCTCTGAAAGGGAATCAAGTCAATCTGATTGTCATATGCGGCATGGGCGGAATTGGCAAGACAACAATGGTGAAAGAAGTTGTTATCAGAGCAAAGAAGGAGGGTCTAATTGATGAGCATGCAAAGGTAGTTGTTAATGACAAACCTGATATAAGTAAGATTCAATCTGATATTGCAGAATTGCTGGGCCTGAAACTTGAAGAACAGGTTTTAGAATCAAGAGCAGATAAGTTATTTAATAGATTATCTGCCAAGAGAGTTCTTGTAGTATTAGATAATGTTTGGGAAACGCTGGATTTGCGGGAAATAGGGATTCCCAGTGGTCTAAACAGTTGTAAAATCTTGGTAACATCACGAAACCAAGATATCTTCAATTATATGGAAGAAGATAGAAAGAAATTCCCCATTGGCGTTTTGCCCGAAAGTGATGCATGGAGTCTGTTTAGGAAGATGGCTGGAGATGACATTGAATCTGATCCCGAGCTGCGTTCTATAGCAGAACAGGTACTCGAGAAATGTGATGGATTACCAGTTGCAATTTCGACTCTGGGAAGCGCACTACGGCGGAAAACGAAAGCGGTCTGGAATGATGCACTCAGACTATTACAAAAGTCTTTCGGGGGAGATATTCAAGGAATGAAGAAGAACGTGTATCAGTCAATCAGGTTGAGTTACGACTTTCTGGAAAGAGAGGAGGCCAAATCCTGTTTTCTGTTGTGTTGCATATTTCGAGAAAGCACTAATATATGTGTTGAAGATTTGGTGACGCATGGATTTGGTCTTGGGGTCTTACATAGAATTGATTCAGTTGAAGGGGGAAGGGATCGTGTAGAAGCCTTGGTTGACACACTCAAAAGTTGCTATTTATTGTTGGACAGTGACAAAGAAGAGTGTGTAAGAATGCACGACGTGGTGCGTGCCGTAGGCTTACATATAGCCTCTGCTGAGAGTGAAGGCACATTTCTTATAAGACACGGAGTGGCATCGGAGAATTGGACCCGGGGATTCATTTGTACATCAATGACCGCCAATAAAAGTACTCATGAGCTTTTGTTGTTGTCATGCACAGATGATGAAAAGAGGTCCGATGTGGACACACTAGGCACATTGTACGGAGTGGAAGATCTTAAGGTCTTGGACATCTCAGTTCCTCATAAAGGTCCTTATACATGGATAACGTTTGCTATTATGTCTAGAACAAGATTAGAATCACTTCCGGTACTTATGAGAAACATTCAAACACTGTGTCTAAGGGGTTTAGAACTCAAATTGGAAACAATTTCTATGATTGGGGACCTACGGACACTCACGATACTCCGCCTAGGTGGATCTTCCATACAGCATGTACCTGAGGAATTTAAAAACTTGTGCAATCTAAAGTTGCTAAATTTGGCGGATTGTTCGGAACTTCAAGAAATCTCACCAGGTGTCATATCAAGTTTAGCCAAACTAGAAGAATTGTACTTGTGGGGTAGCTTCGAGGACTGGAATGATGTGCGGATTATTCAACTTTCATCGGAGCTACTGCAGTTGTCTTTGTTGACGAGTTTAGAAACCACTCTGTCTGTTGATATTCTGCAAAACAGCAGGCATCTATTTGCTAAGCTTAAAAGATTTAGGCTCTGTATACTAAAAAATAAAAGACGTGTACCCAAGTTTCTCCGCAGTATACCACTTTACAAATTGCCTGCAAATCCTCGTGACAACCTCTTGAGACTTGAAGATCTTGGCGCAGACTGCTCTCTTCTGGAAGTCTCTGTGTTGCTAAAGACAACTCATTTTCTACACCTAGGAAATGAAACTTTGGCCCACCCTTTCAATGTAGCAGATGCAGAGTGCGGTAGAAACTTGAAGTGTCTTACGGTCAGAGACTGTGATGCTCTGGAATATCTCGTTGACTTGACACGTGCTGAAACTACTCCTGTAGTCTTTCCTGTCTTGGCTTCACTGAAGATCTATGGTGCACAGAGGTTGCAGGAGATTTTTCATGGATTACTGCCAGTGGGGTCGCTTAAATCACTACGACGTTTAATCATACACCGGGCGGATGAATTAAGGCATGTTTGGAAGACAGAAAGCCAGTTTGATCGCCTGGAAAGTCTAACATTAACCGATTGCCAATCGTTGCAAGTTATTGTTGCTTGGCAAGAATCGGGACATCATCACGAGGAAGAAGCCAAGGAGATCAACTTCCCTAATTTGACACAGTTGGAACTTGCAGAACTACCAAGCCTCGTTGGGATATGTAGCAAAAAAGTCTGCAAGCTCAACTTCTCTAAATTGTTAGATTTACAACTCAGAACGCTTCCGCGATTCAGTGGTCAGTGGTTGAATGGTTTGGCTCCAACAAGTAACTCTAATGACGTCGATGTACAACATCTATTCGACCCCAAGGTTGATTTTCCACTCTTGAGACTTCTGTATCTTGAACATATGCACAACTTTGAAGAGATATGGCACAGCCAACTCTCTCCCTACTCATTCTGCGAACTAAGAGAACTAAAGGTGGAAAGTTGTGACAAAATATCACATTTGGTACCAACATATATGCAAAACAGATTGCAGAAATTGGAGAAGATACATGTGTCCAAGTGTTCTTCGCTACAAGAGATTTTTGAATTGAGGAGGTCGAGGGTCAACGAAGATGAAGGATCAAGGGAAACACCGTCAAATATTTACCAACCTGATCAAGGAATACTACAAATAAATAATCAGATCATGGATTTCAAAATCTTTCAGCATCTAACAAATTTATCCGTCCATAGATGTGAAAGCTTGAGAAAAGTGTGCTCTTCTTCCATTGCAAGAAGTCTTGTGAACCTCGAAAAACTAGAAATCAGTGAGTGCAACAGGTTGGAAGAACTAGTAGCAGCAGAAGAGGGTGAAGAAGCAGAGGATAACAACATGTTCCCTCATCTCAGACTTTTGACTCTTCAATGTCTACCAAATCTCGTAAGTTTTTCCAGAGAAAAATGTGCTTTAGATTGGCCATCCATGGAAGACATAACTATAAAGGACTGCCCCGAAATGAAGAAATTCTGTTCAGGATCGCTTACCACAGCAATGAAAATGAAAATAAAAATAAGTGGTGAAGTTGATGAGAATATTCACAGGGAGCCTGACATGAATAAGAGGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1213

Amino Acids

137.83

Weight (kDa)

5.93

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 158 - 319 9.4e-31 NB-ARC domain
LRR_8 PF13855 600 - 655 1.6e-06 Leucine rich repeat
LRR_RPS2 PF23247 821 - 931 3.2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 975 - 1048 7.5e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1074 - 1188 6.5e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1466
AatII GACGTC 1 cut(s) 2865
Acc65I GGTACC 1 cut(s) 3034
AccB1I GGYRCC 1 cut(s) 3034
AccI GTMKAC 3 cut(s) 2125, 2167, 3439
AccII CGCG 2 cut(s) 147, 2809
AccIII TCCGGA 1 cut(s) 361
AclI AACGTT 2 cut(s) 99, 1676
AclWI GGATC 6 cut(s) 237, 968, 1329, 1834, 3157, 3553
AcoI YGGCCR 1 cut(s) 3485
AcsI RAATTY 7 cut(s) 181, 457, 896, 1853, 1885, 3247, 3533
AcuI CTGAAG 1 cut(s) 2468
AcvI CACGTG 1 cut(s) 2405
AcyI GRCGYC 1 cut(s) 2862
AdeI CACNNNGTG 3 cut(s) 1408, 1477, 1925
AfiI CCNNNNNNNGG 3 cut(s) 339, 1655, 3188
AflII CTTAAG 1 cut(s) 1625
AflIII ACRYGT 6 cut(s) 254, 1146, 2143, 2402, 3079, 3402
AgeI ACCGGT 1 cut(s) 55
AhlI ACTAGT 1 cut(s) 3361
AjiI CACGTC 3 cut(s) 151, 1408, 2144
AjnI CCWGG 5 cut(s) 176, 222, 438, 1921, 2589
Alw21I GWGCWC 2 cut(s) 2464, 3290
Alw26I GTCTC 7 cut(s) 113, 182, 237, 2206, 2257, 2361, 2905
Alw44I GTGCAC 1 cut(s) 2460
AlwI GGATC 6 cut(s) 237, 968, 1329, 1834, 3157, 3553
AlwNI CAGNNNCTG 1 cut(s) 2370
Ama87I CYCGRG 3 cut(s) 977, 1007, 1497
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 5 cut(s) 426, 685, 1194, 2304, 3485
ApaLI GTGCAC 1 cut(s) 2460
ApeKI GCWGC 5 cut(s) 170, 291, 410, 982, 3368
ApoI RAATTY 7 cut(s) 181, 457, 896, 1853, 1885, 3247, 3533
ArsI GACNNNNNNTTYG 2 cut(s) 1729, 1761
AsiGI ACCGGT 1 cut(s) 55
Asp700I GAANNNNTTC 4 cut(s) 57, 1300, 1710, 3533
Asp718I GGTACC 1 cut(s) 3034
AspA2I CCTAGG 2 cut(s) 1819, 2287
AspLEI GCGC 2 cut(s) 1055, 2231
AspS9I GGNCC 8 cut(s) 220, 426, 685, 1494, 1581, 1658, 1792, 2305
AsuC2I CCSGG 3 cut(s) 1498, 1499, 2540
AsuHPI GGTGA 6 cut(s) 191, 580, 1273, 1911, 3392, 3599
AvaI CYCGRG 3 cut(s) 977, 1007, 1497
AvaII GGWCC 5 cut(s) 220, 1494, 1581, 1658, 1792
AvrII CCTAGG 2 cut(s) 1819, 2287
AxyI CCTNAGG 1 cut(s) 1848
BaeGI GKGCMC 1 cut(s) 2464
BaeI ACNNNNGTAYC 4 cut(s) 2903, 2936, 3068, 3101
BalI TGGCCA 1 cut(s) 3487
BanI GGYRCC 1 cut(s) 3034
BauI CACGAG 2 cut(s) 2196, 2663
BbrPI CACGTG 1 cut(s) 2405
BbsI GAAGAC 2 cut(s) 2573, 3504
Bbv12I GWGCWC 2 cut(s) 2464, 3290
BbvI GCAGC 5 cut(s) 182, 278, 397, 969, 3380
BccI CCATC 4 cut(s) 487, 943, 1013, 3496
BceAI ACGGC 3 cut(s) 341, 1076, 1403
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 5 cut(s) 178, 224, 440, 1923, 2591
BciVI GTATCC 1 cut(s) 244
BclI TGATCA 1 cut(s) 3184
BcnI CCSGG 3 cut(s) 1498, 1499, 2540
BcoDI GTCTC 7 cut(s) 113, 182, 237, 2206, 2257, 2361, 2905
BcuI ACTAGT 1 cut(s) 3361
BfaI CTAG 8 cut(s) 35, 1598, 1692, 1820, 1949, 2288, 3329, 3362
BfmI CTRYAG 3 cut(s) 990, 2024, 2421
BfrI CTTAAG 1 cut(s) 1625
BfuI GTATCC 1 cut(s) 244
BglII AGATCT 4 cut(s) 210, 1621, 2221, 2451
BisI GCNGC 6 cut(s) 171, 292, 411, 538, 983, 3369
BlnI CCTAGG 2 cut(s) 1819, 2287
BlpI GCTNAGC 1 cut(s) 2100
BlsI GCNGC 6 cut(s) 172, 293, 412, 539, 984, 3370
BmcAI AGTACT 1 cut(s) 1537
Bme1390I CCNGG 8 cut(s) 178, 224, 440, 1498, 1499, 1923, 2540, 2591
Bme18I GGWCC 5 cut(s) 220, 1494, 1581, 1658, 1792
BmeT110I CYCGRG 3 cut(s) 977, 1007, 1497
BmgBI CACGTC 3 cut(s) 151, 1408, 2144
BmgT120I GGNCC 8 cut(s) 220, 426, 685, 1494, 1581, 1658, 1792, 2305
BmiI GGNNCC 7 cut(s) 308, 427, 1496, 1793, 2841, 3036, 3615
BmrFI CCNGG 8 cut(s) 178, 224, 440, 1498, 1499, 1923, 2540, 2591
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 8 cut(s) 452, 916, 1078, 1490, 2098, 2317, 2365, 3247
BmuI ACTGGG 1 cut(s) 810
BpiI GAAGAC 2 cut(s) 2573, 3504
BpmI CTGGAG 1 cut(s) 975
Bpu1102I GCTNAGC 1 cut(s) 2100
BpuEI CTTGAG 4 cut(s) 355, 2230, 2929, 3295
BpuMI CCSGG 3 cut(s) 1498, 1499, 2540
BsaAI YACGTR 1 cut(s) 2405
BsaBI GATNNNNATC 1 cut(s) 1705
BsaHI GRCGYC 1 cut(s) 2862
BsaJI CCNNGG 9 cut(s) 438, 1335, 1497, 1498, 1819, 2287, 2676, 2889, 3492
BsaWI WCCGGW 3 cut(s) 55, 361, 1714
Bsc4I CCNNNNNNNGG 3 cut(s) 339, 1655, 3188
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 5 cut(s) 816, 1028, 1988, 2498, 2578
Bse21I CCTNAGG 1 cut(s) 1848
Bse3DI GCAATG 2 cut(s) 3297, 3567
Bse8I GATNNNNATC 1 cut(s) 1705
BseAI TCCGGA 1 cut(s) 361
BseBI CCWGG 5 cut(s) 178, 224, 440, 1923, 2591
BseDI CCNNGG 9 cut(s) 438, 1335, 1497, 1498, 1819, 2287, 2676, 2889, 3492
BseGI GGATG 4 cut(s) 370, 443, 2551, 3488
BseJI GATNNNNATC 1 cut(s) 1705
BseLI CCNNNNNNNGG 3 cut(s) 339, 1655, 3188
BseMI GCAATG 2 cut(s) 3297, 3567
BseMII CTCAG 6 cut(s) 1108, 1434, 1656, 1839, 2809, 3430
BseNI ACTGG 5 cut(s) 816, 1028, 1988, 2498, 2578
BseRI GAGGAG 3 cut(s) 424, 1205, 3136
BseSI GKGCMC 1 cut(s) 2464
BseXI GCAGC 5 cut(s) 182, 278, 397, 969, 3380
BseYI CCCAGC 1 cut(s) 682
Bsh1236I CGCG 2 cut(s) 147, 2809
BshFI GGCC 5 cut(s) 428, 687, 1196, 2306, 3487
BshNI GGYRCC 1 cut(s) 3034
BshTI ACCGGT 1 cut(s) 55
BsiHKAI GWGCWC 2 cut(s) 2464, 3290
BsiHKCI CYCGRG 3 cut(s) 977, 1007, 1497
BsiSI CCGG 5 cut(s) 56, 362, 1498, 1715, 2539
BslFI GGGAC 2 cut(s) 1805, 2667
BslI CCNNNNNNNGG 3 cut(s) 339, 1655, 3188
BsmAI GTCTC 7 cut(s) 113, 182, 237, 2206, 2257, 2361, 2905
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 2 cut(s) 1805, 2667
BsmI GAATGC 4 cut(s) 139, 293, 328, 1404
BsnI GGCC 5 cut(s) 428, 687, 1196, 2306, 3487
BsoBI CYCGRG 3 cut(s) 977, 1007, 1497
Bsp1286I GDGCHC 2 cut(s) 2464, 3290
Bsp13I TCCGGA 1 cut(s) 361
Bsp1407I TGTACA 2 cut(s) 1511, 2869
Bsp1720I GCTNAGC 1 cut(s) 2100
Bsp19I CCATGG 1 cut(s) 3492
BspANI GGCC 5 cut(s) 428, 687, 1196, 2306, 3487
BspCNI CTCAG 6 cut(s) 1107, 1435, 1655, 1840, 2808, 3429
BspEI TCCGGA 1 cut(s) 361
BspFNI CGCG 2 cut(s) 147, 2809
BspHI TCATGA 1 cut(s) 1540
BspLI GGNNCC 7 cut(s) 308, 427, 1496, 1793, 2841, 3036, 3615
BspMAI CTGCAG 1 cut(s) 2028
BspPI GGATC 6 cut(s) 237, 968, 1329, 1834, 3157, 3553
BspQI GCTCTTC 1 cut(s) 3295
BspT107I GGYRCC 1 cut(s) 3034
BspTI CTTAAG 1 cut(s) 1625
BsrDI GCAATG 2 cut(s) 3297, 3567
BsrFI RCCGGY 1 cut(s) 55
BsrGI TGTACA 2 cut(s) 1511, 2869
BsrI ACTGG 5 cut(s) 816, 1028, 1988, 2498, 2578
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 9 cut(s) 438, 1335, 1497, 1498, 1819, 2287, 2676, 2889, 3492
BssNAI GTATAC 2 cut(s) 2126, 2168
BssNI GRCGYC 1 cut(s) 2862
BssSI CACGAG 2 cut(s) 2196, 2663
BssT1I CCWWGG 6 cut(s) 1335, 1819, 2287, 2676, 2889, 3492
Bst1107I GTATAC 2 cut(s) 2126, 2168
Bst2BI CACGAG 2 cut(s) 2196, 2663
Bst2UI CCWGG 5 cut(s) 178, 224, 440, 1923, 2591
Bst4CI ACNGT 9 cut(s) 49, 69, 830, 1376, 1743, 2362, 2372, 2706, 3165
Bst6I CTCTTC 6 cut(s) 1380, 2247, 2940, 3295, 3369, 3435
BstACI GRCGYC 1 cut(s) 2862
BstAFI CTTAAG 1 cut(s) 1625
BstAUI TGTACA 2 cut(s) 1511, 2869
BstBAI YACGTR 1 cut(s) 2405
BstC8I GCNNGC 6 cut(s) 430, 618, 1416, 2087, 2187, 2762
BstDEI CTNAG 9 cut(s) 1094, 1443, 1642, 1748, 1848, 2100, 2795, 2990, 3416
BstDSI CCRYGG 1 cut(s) 3492
BstENI CCTNNNNNAGG 3 cut(s) 337, 1653, 3186
BstF5I GGATG 4 cut(s) 370, 443, 2551, 3488
BstFNI CGCG 2 cut(s) 147, 2809
BstHHI GCGC 2 cut(s) 1055, 2231
BstMAI GTCTC 7 cut(s) 113, 182, 237, 2206, 2257, 2361, 2905
BstMWI GCNNNNNNNGC 5 cut(s) 300, 543, 552, 796, 2235
BstNI CCWGG 5 cut(s) 178, 224, 440, 1923, 2591
BstNSI RCATGY 6 cut(s) 258, 620, 1844, 2561, 3083, 3406
BstSCI CCNGG 8 cut(s) 176, 222, 438, 1496, 1497, 1921, 2538, 2589
BstSFI CTRYAG 3 cut(s) 990, 2024, 2421
BstSLI GKGCMC 1 cut(s) 2464
BstUI CGCG 2 cut(s) 147, 2809
BstV1I GCAGC 5 cut(s) 182, 278, 397, 969, 3380
BstV2I GAAGAC 2 cut(s) 2573, 3504
BstX2I RGATCY 5 cut(s) 210, 1621, 1826, 2221, 2451
BstYI RGATCY 5 cut(s) 210, 1621, 1826, 2221, 2451
BstZ17I GTATAC 2 cut(s) 2126, 2168
Bsu36I CCTNAGG 1 cut(s) 1848
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 5 cut(s) 428, 687, 1196, 2306, 3487
BtgI CCRYGG 1 cut(s) 3492
BtrI CACGTC 3 cut(s) 151, 1408, 2144
BtsCI GGATG 4 cut(s) 370, 443, 2551, 3488
BtsIMutI CAGTG 8 cut(s) 823, 1381, 1739, 2444, 2505, 2821, 2828, 3343
Cac8I GCNNGC 6 cut(s) 430, 618, 1416, 2087, 2187, 2762
CaiI CAGNNNCTG 1 cut(s) 2370
CciI TCATGA 1 cut(s) 1540
CfoI GCGC 2 cut(s) 1055, 2231
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 8 cut(s) 220, 426, 685, 1494, 1581, 1658, 1792, 2305
Cfr9I CCCGGG 1 cut(s) 1497
CseI GACGC 2 cut(s) 153, 1273
CsiI ACCWGGT 2 cut(s) 222, 1921
CspAI ACCGGT 1 cut(s) 55
DdeI CTNAG 9 cut(s) 1094, 1443, 1642, 1748, 1848, 2100, 2795, 2990, 3416
DraI TTTAAA 1 cut(s) 1858
DraIII CACNNNGTG 3 cut(s) 1408, 1477, 1925
EaeI YGGCCR 1 cut(s) 3485
Eam1104I CTCTTC 6 cut(s) 1380, 2247, 2940, 3295, 3369, 3435
EarI CTCTTC 6 cut(s) 1380, 2247, 2940, 3295, 3369, 3435
EciI GGCGGA 6 cut(s) 299, 561, 1078, 1805, 1907, 2558
Eco130I CCWWGG 6 cut(s) 1335, 1819, 2287, 2676, 2889, 3492
Eco32I GATATC 1 cut(s) 865
Eco47I GGWCC 5 cut(s) 220, 1494, 1581, 1658, 1792
Eco57I CTGAAG 1 cut(s) 2468
Eco72I CACGTG 1 cut(s) 2405
Eco81I CCTNAGG 1 cut(s) 1848
Eco88I CYCGRG 3 cut(s) 977, 1007, 1497
EcoNI CCTNNNNNAGG 3 cut(s) 337, 1653, 3186
EcoO109I RGGNCCY 2 cut(s) 1658, 1792
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 5 cut(s) 176, 222, 438, 1921, 2589
EcoRV GATATC 1 cut(s) 865
EcoT14I CCWWGG 6 cut(s) 1335, 1819, 2287, 2676, 2889, 3492
EcoT22I ATGCAT 1 cut(s) 931
ErhI CCWWGG 6 cut(s) 1335, 1819, 2287, 2676, 2889, 3492
Esp3I CGTCTC 1 cut(s) 113
FalI AAGNNNNNCTT 2 cut(s) 2753, 2785
FaqI GGGAC 2 cut(s) 1805, 2667
FauI CCCGC 1 cut(s) 792
FauNDI CATATG 2 cut(s) 533, 2931
FbaI TGATCA 1 cut(s) 3184
FblI GTMKAC 3 cut(s) 2125, 2167, 3439
Fnu4HI GCNGC 6 cut(s) 171, 292, 411, 538, 983, 3369
FokI GGATG 4 cut(s) 377, 430, 2558, 3475
Fsp4HI GCNGC 6 cut(s) 171, 292, 411, 538, 983, 3369
FspBI CTAG 8 cut(s) 35, 1598, 1692, 1820, 1949, 2288, 3329, 3362
GlaI GCGC 2 cut(s) 1054, 2230
GluI GCNGC 6 cut(s) 171, 292, 411, 538, 983, 3369
GsaI CCCAGC 1 cut(s) 686
GsuI CTGGAG 1 cut(s) 975
HaeIII GGCC 5 cut(s) 428, 687, 1196, 2306, 3487
HapII CCGG 5 cut(s) 56, 362, 1498, 1715, 2539
HgaI GACGC 2 cut(s) 153, 1273
HhaI GCGC 2 cut(s) 1055, 2231
Hin1I GRCGYC 1 cut(s) 2862
Hin6I GCGC 2 cut(s) 1053, 2229
HinP1I GCGC 2 cut(s) 1053, 2229
HincII GTYRAC 4 cut(s) 1342, 2040, 2395, 3136
HindII GTYRAC 4 cut(s) 1342, 2040, 2395, 3136
HindIII AAGCTT 2 cut(s) 2102, 3271
HpaII CCGG 5 cut(s) 56, 362, 1498, 1715, 2539
HphI GGTGA 6 cut(s) 191, 580, 1273, 1911, 3392, 3599
Hpy99I CGWCG 4 cut(s) 152, 1409, 2526, 2867
HpyAV CCTTC 7 cut(s) 416, 479, 589, 1304, 1311, 1445, 3140
HpyCH4III ACNGT 9 cut(s) 49, 69, 830, 1376, 1743, 2362, 2372, 2706, 3165
HpyCH4IV ACGT 9 cut(s) 99, 150, 1146, 1407, 1676, 2143, 2404, 2524, 2862
HpyF10VI GCNNNNNNNGC 5 cut(s) 300, 543, 552, 796, 2235
HpyF3I CTNAG 9 cut(s) 1094, 1443, 1642, 1748, 1848, 2100, 2795, 2990, 3416
HpySE526I ACGT 9 cut(s) 99, 150, 1146, 1407, 1676, 2143, 2404, 2524, 2862
Hsp92I GRCGYC 1 cut(s) 2862
HspAI GCGC 2 cut(s) 1053, 2229
Kpn2I TCCGGA 1 cut(s) 361
KpnI GGTACC 1 cut(s) 3038
Ksp22I TGATCA 1 cut(s) 3184
LguI GCTCTTC 1 cut(s) 3295
LmnI GCTCC 4 cut(s) 437, 2017, 2845, 3613
Lsp1109I GCAGC 5 cut(s) 182, 278, 397, 969, 3380
LweI GCATC 8 cut(s) 452, 916, 1078, 1490, 2098, 2317, 2365, 3247
MabI ACCWGGT 2 cut(s) 222, 1921
MaeI CTAG 8 cut(s) 35, 1598, 1692, 1820, 1949, 2288, 3329, 3362
MaeII ACGT 9 cut(s) 99, 150, 1146, 1407, 1676, 2143, 2404, 2524, 2862
MaeIII GTNAC 8 cut(s) 95, 844, 1169, 1261, 1376, 2198, 2849, 3014
MflI RGATCY 5 cut(s) 210, 1621, 1826, 2221, 2451
MhlI GDGCHC 2 cut(s) 2464, 3290
MlsI TGGCCA 1 cut(s) 3487
MluNI TGGCCA 1 cut(s) 3487
MlyI GAGTC 4 cut(s) 352, 943, 1036, 3421
MmeI TCCRAC 6 cut(s) 198, 471, 1350, 2688, 2867, 3333
Mox20I TGGCCA 1 cut(s) 3487
Mph1103I ATGCAT 1 cut(s) 931
MroI TCCGGA 1 cut(s) 361
MroXI GAANNNNTTC 4 cut(s) 57, 1300, 1710, 3533
MscI TGGCCA 1 cut(s) 3487
MseI TTAA 9 cut(s) 633, 735, 1626, 1857, 2106, 2511, 2528, 2552, 2606
MslI CAYNNNNRTG 2 cut(s) 3264, 3560
Msp20I TGGCCA 1 cut(s) 3487
MspCI CTTAAG 1 cut(s) 1625
MspI CCGG 5 cut(s) 56, 362, 1498, 1715, 2539
MspR9I CCNGG 8 cut(s) 178, 224, 440, 1498, 1499, 1923, 2540, 2591
Mva1269I GAATGC 4 cut(s) 139, 293, 328, 1404
MvaI CCWGG 5 cut(s) 178, 224, 440, 1923, 2591
MvnI CGCG 2 cut(s) 147, 2809
MwoI GCNNNNNNNGC 5 cut(s) 300, 543, 552, 796, 2235
NciI CCSGG 3 cut(s) 1498, 1499, 2540
NcoI CCATGG 1 cut(s) 3492
NdeI CATATG 2 cut(s) 533, 2931
NlaIV GGNNCC 7 cut(s) 308, 427, 1496, 1793, 2841, 3036, 3615
NmuCI GTSAC 4 cut(s) 1261, 1376, 2198, 3014
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 6 cut(s) 258, 620, 1844, 2561, 3083, 3406
PaeI GCATGC 1 cut(s) 620
PaeR7I CTCGAG 1 cut(s) 1007
PagI TCATGA 1 cut(s) 1540
PciI ACATGT 3 cut(s) 254, 3079, 3402
PciSI GCTCTTC 1 cut(s) 3295
PcsI WCGNNNNNNNCGW 1 cut(s) 238
PctI GAATGC 4 cut(s) 139, 293, 328, 1404
PdmI GAANNNNTTC 4 cut(s) 57, 1300, 1710, 3533
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 6 cut(s) 172, 293, 412, 539, 984, 3370
PleI GAGTC 4 cut(s) 352, 942, 1036, 3421
PmaCI CACGTG 1 cut(s) 2405
PmlI CACGTG 1 cut(s) 2405
PpsI GAGTC 4 cut(s) 352, 942, 1036, 3421
Ppu21I YACGTR 1 cut(s) 2405
PpuMI RGGWCCY 2 cut(s) 1658, 1792
PscI ACATGT 3 cut(s) 254, 3079, 3402
PsiI TTATAA 1 cut(s) 1466
Psp1406I AACGTT 2 cut(s) 99, 1676
Psp5II RGGWCCY 2 cut(s) 1658, 1792
Psp6I CCWGG 5 cut(s) 176, 222, 438, 1921, 2589
PspCI CACGTG 1 cut(s) 2405
PspFI CCCAGC 1 cut(s) 682
PspGI CCWGG 5 cut(s) 176, 222, 438, 1921, 2589
PspN4I GGNNCC 7 cut(s) 308, 427, 1496, 1793, 2841, 3036, 3615
PspPI GGNCC 8 cut(s) 220, 426, 685, 1494, 1581, 1658, 1792, 2305
PspPPI RGGWCCY 2 cut(s) 1658, 1792
PstI CTGCAG 1 cut(s) 2028
PstNI CAGNNNCTG 1 cut(s) 2370
PsuI RGATCY 5 cut(s) 210, 1621, 1826, 2221, 2451
RseI CAYNNNNRTG 2 cut(s) 3264, 3560
SapI GCTCTTC 1 cut(s) 3295
SaqAI TTAA 9 cut(s) 633, 735, 1626, 1857, 2106, 2511, 2528, 2552, 2606
SatI GCNGC 6 cut(s) 171, 292, 411, 538, 983, 3369
Sau96I GGNCC 8 cut(s) 220, 426, 685, 1494, 1581, 1658, 1792, 2305
ScaI AGTACT 1 cut(s) 1537
SchI GAGTC 4 cut(s) 352, 943, 1036, 3421
ScrFI CCNGG 8 cut(s) 178, 224, 440, 1498, 1499, 1923, 2540, 2591
SduI GDGCHC 2 cut(s) 2464, 3290
SexAI ACCWGGT 2 cut(s) 222, 1921
SfaNI GCATC 8 cut(s) 452, 916, 1078, 1490, 2098, 2317, 2365, 3247
SfcI CTRYAG 3 cut(s) 990, 2024, 2421
Sfr274I CTCGAG 1 cut(s) 1007
SinI GGWCC 5 cut(s) 220, 1494, 1581, 1658, 1792
SlaI CTCGAG 1 cut(s) 1007
SmaI CCCGGG 1 cut(s) 1499
SmiMI CAYNNNNRTG 2 cut(s) 3264, 3560
SmlI CTYRAG 6 cut(s) 334, 1007, 1625, 2209, 2908, 3274
SmoI CTYRAG 6 cut(s) 334, 1007, 1625, 2209, 2908, 3274
SpeI ACTAGT 1 cut(s) 3361
SphI GCATGC 1 cut(s) 620
SspI AATATT 2 cut(s) 3172, 3604
SspMI CTAG 8 cut(s) 35, 1598, 1692, 1820, 1949, 2288, 3329, 3362
StyD4I CCNGG 8 cut(s) 176, 222, 438, 1496, 1497, 1921, 2538, 2589
StyI CCWWGG 6 cut(s) 1335, 1819, 2287, 2676, 2889, 3492
TaaI ACNGT 9 cut(s) 49, 69, 830, 1376, 1743, 2362, 2372, 2706, 3165
TaiI ACGT 9 cut(s) 102, 153, 1149, 1410, 1679, 2146, 2407, 2527, 2865
TatI WGTACW 5 cut(s) 42, 1511, 1535, 1959, 2869
TauI GCSGC 1 cut(s) 540
Tru1I TTAA 9 cut(s) 633, 735, 1626, 1857, 2106, 2511, 2528, 2552, 2606
Tru9I TTAA 9 cut(s) 633, 735, 1626, 1857, 2106, 2511, 2528, 2552, 2606
TscAI CASTG 8 cut(s) 823, 1381, 1746, 2451, 2505, 2821, 2828, 3343
TseFI GTSAC 4 cut(s) 1261, 1376, 2198, 3014
TseI GCWGC 5 cut(s) 170, 291, 410, 982, 3368
Tsp45I GTSAC 4 cut(s) 1261, 1376, 2198, 3014
TspGWI ACGGA 4 cut(s) 1488, 1626, 1813, 3244
TspMI CCCGGG 1 cut(s) 1497
TspRI CASTG 8 cut(s) 823, 1381, 1746, 2451, 2505, 2821, 2828, 3343
Vha464I CTTAAG 1 cut(s) 1625
VneI GTGCAC 1 cut(s) 2460
VpaK11BI GGWCC 5 cut(s) 220, 1494, 1581, 1658, 1792
XagI CCTNNNNNAGG 3 cut(s) 337, 1653, 3186
XapI RAATTY 7 cut(s) 181, 457, 896, 1853, 1885, 3247, 3533
XbaI TCTAGA 1 cut(s) 1691
XceI RCATGY 6 cut(s) 258, 620, 1844, 2561, 3083, 3406
XhoI CTCGAG 1 cut(s) 1007
XmaI CCCGGG 1 cut(s) 1497
XmaJI CCTAGG 2 cut(s) 1819, 2287
XmiI GTMKAC 3 cut(s) 2125, 2167, 3439
XmnI GAANNNNTTC 4 cut(s) 57, 1300, 1710, 3533
XspI CTAG 8 cut(s) 35, 1598, 1692, 1820, 1949, 2288, 3329, 3362
ZraI GACGTC 1 cut(s) 2863
ZrmI AGTACT 1 cut(s) 1537
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.