Rh1BG139600

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000203
Physical Location & Seq
Forward (+)
23265255 .. 23271721
6467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG139600.1

Sequence Viewer

Length: 3912 bp
ATGCAAGATATTATTCTAGCAGTTGCTACACGATTGGCAGAATGGACTGTGAGACCAATTTGGCAGCAGTTCAGTTATGTGATTCAGTACAAGAGCAACATTGAGCATCTCACCAGACAGGTTCAGGAACTGCATGATAAAAGATATGGAGTGGACATGGAGGTGAAACCTGCTACAGAAGCTTCGAAGATTATTGATCCTGGAGTTAAACATTGGCTGGCTGAGGTAGACAAGCTGATAGGAGAAAAGGAGAAGTGTTTCAATGAAGAGACTGTAGCAACAAAGGCAGCATGCTGTAATGGGTCTTTTCCAGATTTGAAGTGCCGTTATTCTTTAGGAAGGAAAGCTAAGAAGATGACTATGGTTTTTGATTACCTCCTTGCTAAAGATCTCAGGACTATAGCGCATCCTGCTCTTCCCCCAGAGGTGGAATTTCAACCCACACAATATTCTGAGGATTCTTCTCAGCTTGCAACCAACTTCCAGGAAGGTGCCAGCTCCTCAGAAACATCTAACCAAATTCAGCCGCCTACATCTACAGGAAAAAATATTGTTTTTGAATCGAGGAGATCGACCATTAGAAGTGTAATGGAAGCTGTGAAGGGGAATGAGATCAATCCGGTTGTCATATGCGGCATGGGGGGTATTGGGAAGACAACATTGATGGGGGAAGTTGTTAACAGAGCAAAGAAAGAGGGTCTGTTTGATGAGTATACAAAGGCAACTGTTAAAGAAACTCTTGACAAAAATCCTGACATTTGTCTGATTCAAGATGAACTTGCTCAATATCTAGGTCTTCCACTTGAAGGAAAAGAAATAGATGCAAGAGCAAACAAGATACGTCAAAGATTATCTGCAGGTGCCAAGAAGGTTCTTGTAATATTAGACAATGTTTCGACAACAAACCCAGACTTTTTGTGGGAAGTAGGGATTCCTCGTGATCAAAATAGTTGTAAGCTTTTAGTAACATCAAGAGAACGAGATGTATTCAAGGACATTGACACAAAAAAGAATTTCCCCATCAGTGATTTGCCAGAAAATGAGGCCTGGAGCTTGTTCAAGAAGACAGCAGGAGGTTTGATTGAATCTGATCCTGATTTGCATTTGGTAGCAAAACAGGTTGTGAAGGAATGTGCAGGGTTGCCAATTGCAATTTCAACTGTTGGAAGGGCACTGCATGGTGAAGGAATTGCAATTTGGAAGAATGCACTTAGGGAAATGGAAAAGGCTTGCCCAGAAAATGTTCCAGGAGTGCTAGATCATGTGTATGGAAAAATAAAATTCAGTTATGAATGTTTACAAAGCAAGCAAGCCAAATCATGCTTTCTGTTATGTTGCATATTTGCAGAAAGCGTTGATATACCGATTGAATATTTTGTTAGATTTGGGCTTGGGCTTGGGATCTTTAAAGGCATTGATTCAATGGCTGAAGGAAGAAACTGTCTAGAAACATTGGTTCACACATTAAAAAGTCGCTTTCTATTGTTGGACAGTGATAAGAAAGAGTGTGTGAGAATGCATGATGTGGTGCACGATGTAGCCTTGCATATAGCCTCTGAAGGGTTGACTCAAAAGAAAAGTGATGAGGTTAAAGAGAGAATTGTGGTAAGACACTCAGTGGAAACTGTGAAGTCTATGGATTGGCCAAAGAGTAGTGTTTCCGAGCAATCTACTTACTGCAGTTCACTGTGTATTGGTGGGCTTTCGGAGCTCTTACTGTTAAGATGCATAACTGAAAGTGGATTGGAGCCGCCACCCACAATTTTCAATAGAATGGAAGATCTCAAGGTTCTCGTAATTGCAGGTATTTCTCTATTGCCAATACTGCCATCACTTCCAGTTTTGAGAAACCTTCAAACATTGTGTTTGGAGGATTGCTATCTAAACTTTGATGTAGTATCTGTCATTGGGGAGATAAGGACACTCATGATACTCAGTTTACGTGGATCTGACATCAAACAACTGACTGATACATTTAAAAATTTATCTAATCTAAGATTACTTGATTTGTCGAGATGTATGAAACTTGAGATAATCTCACCAGGTGTCATATCAAGTTTATCCCGACTAGAAGAGTTGTATATGTGGGATAGCTTTGAAGATTGGGTGGTTGAGAAACTAGCCTCAACAGAAACAACCAATTGGATTTCTCAATTGGAAGCTGAGACAAGAGCTAAACCAGAAGAACTCCACATGGAACTGGAGATGTGGAAGAGATTCAACAGATTGGCGGTTGAGCAACCAGCATATCTAGGAAAGATGTACAGAGAGGAGGTTCAAAATTTATATACGAAAGACGAGGAGCACACATACAGAAGGTGGGCCAACTCCATGGACTGGAAAATTGGGATACTTCCAGAGCTACTTTCCTTGTCCAGTTTGACTACTTTAGAAGTAGTTCTACCACCGGTTGATAGTCTGGTAACCAGCAAGCTATTTGACAAGCTTGAAAGATTTAAGATCTCTATAGGATGGAAGGAGCATATATATAATTGGGAATTAGAGCCTTATGGAAACTACTTGAGAGTTCATGATCTTGATGCAAACTCTGTTGTGAGTACTGGAATCACTTTGTTGCTAAAGAAAACCAAGACTTTTGAGTTAAAAATGAAGAATTTGACAGAACCTCTAAGACATACAGAGTGCTGTTTTGCAAACTTGAAGTCTCTTGCACTCGAGGAGTGTGAATCTTTGGAATATCTGATTGACATGACACTTGTAAAACCTACACCACATAGTAGTGTCTTTCCTGTCCTGGATCGTCTGGTTGTATTTGGAGGAAGTAGGCTGAAGGAGATTTTTCATGGTGAGCTGCCAATGGGATCTCTTAAGAAACTACGTGAGATTGATTTACTATGTCTACCTTCATTGACCTATATTTGGACGACCGAAAGTCATTCTGAATGCTTGAGAAATATTCTAGGACTCATTGAAGTCCTAAAAATAAAAAACTGCCCGTCGTTGGAAGAGATCATTGCCTTGGAAGGATCAAGACATCAGGAGGAAGAAGCAACCAAAGAGATCAACTTCCAAAGATTGACAGAGTTGACTCTTGCAAATCTACCAAGTTTCACTGGGATAAGCAAAAATATCTATAAAAAGGAGTTGGAAAGGCTACCGGATGTTATCAGTCTCTATCCAAATGTTTTGACAGTGGAACATTCTAATGTGTCCGTACAACATCTGTTTGACACCAAGGTTCAATTTCCAGTTTTACGAAGACTAAAAATTGAAGGCATGGACAACTTAAAAGAGATATGGAACAGTGAACTTTCACCTGTTTCCTTTTGTGAACTAAAGTTTCTAATAGTGAACAGATGTAACAAACTATTGCATTTGGTGCCAACACATATGCAAAATAGATTGCAGAAAATGGAGTTCATATGTGCATCACACTGTTCTTCACTGGAAGAGATCTTTGATTTCAGGAGATCACCTTCACACATTTCCCAACCTGGCCAAGGGACCCAAAGGAACAGGATCATGGATTTCAAACAATCTTGTCAAGCCTTTCAGAATCTAACAGATGTATCACTCTGGGAATGTAACAGTGTTAGAATTGTGCTCTCTCCAGTTTCCAGGGGTCTTGTGAAACTCAAAGAACTAAGGATACGGCGGTGCCAGAAGATTGAAGAAATAGTAGCAGCAGCAGCAGAGAGTGAAGAAATAGAGGATAACATGTTTCCTCAGCTGAGTAAATTGGTACTTGAGGATCTGCCAAATCTTCGAAGCTTTTCACAAGGCAAATATAATTTTGAATGGCCACTGATGAAATATATAGATATTGGAAAATGCTATATGAAGCATTTTTGTTTTGGATCCCTCAGCATTCCAAGGGAAGTGATTATATATGTTAGTGATGCTGGTGAGAATCTTTTGCAGGAGCTCAATAACAGTAGAAAAGAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1303

Amino Acids

148.31

Weight (kDa)

5.94

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATPase_2 PF01637 188 - 301 2.8e-06 ATPase domain predominantly from Archaea
NB-ARC PF00931 193 - 357 3.3e-21 NB-ARC domain
LRR_8 PF13855 640 - 695 2.7e-07 Leucine rich repeat
LRR_RPS2 PF23247 1074 - 1145 3e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1167 - 1245 1.2e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 848
Acc36I ACCTGC 3 cut(s) 178, 848, 1793
AccB1I GGYRCC 4 cut(s) 491, 860, 3340, 3618
AccB7I CCANNNNNTGG 1 cut(s) 2337
AccI GTMKAC 3 cut(s) 228, 713, 2860
AciI CCGC 5 cut(s) 527, 633, 1751, 2231, 3616
AcoI YGGCCR 3 cut(s) 1643, 3457, 3761
AcsI RAATTY 7 cut(s) 431, 519, 1012, 1280, 1981, 2281, 2614
AcuI CTGAAG 3 cut(s) 1449, 1578, 2810
AdeI CACNNNGTG 2 cut(s) 1618, 2045
AfaI GTAC 5 cut(s) 89, 2264, 2560, 3177, 3705
AfiI CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2880, 2962, 3461
AflII CTTAAG 1 cut(s) 2828
AflIII ACRYGT 1 cut(s) 3678
AgeI ACCGGT 1 cut(s) 2407
AhdI GACNNNNNGTC 1 cut(s) 2892
AjnI CCWGG 8 cut(s) 199, 483, 1046, 1246, 2041, 2754, 3454, 3578
AjuI GAANNNNNNNTTGG 4 cut(s) 1138, 1170, 1180, 1212
Alw21I GWGCWC 5 cut(s) 1533, 1713, 2307, 3567, 3888
Alw26I GTCTC 5 cut(s) 46, 263, 2159, 2670, 3137
Alw44I GTGCAC 1 cut(s) 1529
AlwNI CAGNNNCTG 1 cut(s) 130
Ama87I CYCGRG 1 cut(s) 2675
AoxI GGCC 5 cut(s) 1044, 1643, 2322, 3457, 3761
ApaLI GTGCAC 1 cut(s) 1529
ApeKI GCWGC 6 cut(s) 64, 287, 2812, 3644, 3647, 3650
ApoI RAATTY 7 cut(s) 431, 519, 1012, 1280, 1981, 2281, 2614
ArsI GACNNNNNNTTYG 2 cut(s) 2648, 2680
AsiGI ACCGGT 1 cut(s) 2407
Asp700I GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3733
AspLEI GCGC 1 cut(s) 406
AspS9I GGNCC 2 cut(s) 2322, 3465
AsuHPI GGTGA 8 cut(s) 103, 175, 1193, 2031, 2819, 3267, 3426, 3878
AsuII TTCGAA 2 cut(s) 185, 3727
AvaI CYCGRG 1 cut(s) 2675
AvaII GGWCC 1 cut(s) 3465
BaeGI GKGCMC 2 cut(s) 1174, 1533
BaeI ACNNNNGTAYC 4 cut(s) 3513, 3546, 3602, 3635
BalI TGGCCA 3 cut(s) 1645, 3459, 3763
BamHI GGATCC 1 cut(s) 3818
BanI GGYRCC 4 cut(s) 491, 860, 3340, 3618
BanII GRGCYC 2 cut(s) 1713, 3888
BarI GAAGNNNNNNTAC 2 cut(s) 2385, 2417
BauI CACGAG 1 cut(s) 936
BbsI GAAGAC 4 cut(s) 659, 788, 1070, 3226
Bbv12I GWGCWC 5 cut(s) 1533, 1713, 2307, 3567, 3888
BbvCI CCTCAGC 3 cut(s) 222, 3687, 3824
BbvI GCAGC 6 cut(s) 76, 299, 2799, 3656, 3659, 3662
BccI CCATC 4 cut(s) 658, 1028, 1837, 2466
BceAI ACGGC 2 cut(s) 309, 3629
BciT130I CCWGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
BciVI GTATCC 2 cut(s) 2343, 3603
BclI TGATCA 1 cut(s) 940
BcoDI GTCTC 5 cut(s) 46, 263, 2159, 2670, 3137
BfaI CTAG 8 cut(s) 17, 791, 1256, 1445, 2069, 2120, 2252, 2921
BfmI CTRYAG 7 cut(s) 174, 273, 399, 537, 855, 1678, 2466
BfrI CTTAAG 1 cut(s) 2828
BfuAI ACCTGC 3 cut(s) 178, 848, 1793
BfuI GTATCC 2 cut(s) 2343, 3603
BglII AGATCT 4 cut(s) 388, 1780, 2460, 3414
BisI GCNGC 9 cut(s) 65, 288, 527, 634, 1751, 2813, 3645, 3648, 3651
BlsI GCNGC 9 cut(s) 66, 289, 528, 635, 1752, 2814, 3646, 3649, 3652
BmcAI AGTACT 1 cut(s) 2560
Bme1390I CCNGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
Bme18I GGWCC 1 cut(s) 3465
BmeRI GACNNNNNGTC 1 cut(s) 2892
BmeT110I CYCGRG 1 cut(s) 2675
BmgT120I GGNCC 2 cut(s) 2322, 3465
BmiI GGNNCC 8 cut(s) 493, 862, 1749, 3342, 3466, 3467, 3620, 3820
BmrFI CCNGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
BmrI ACTGGG 1 cut(s) 3084
BmsI GCATC 7 cut(s) 115, 415, 811, 1715, 2530, 3398, 3850
BmuI ACTGGG 1 cut(s) 3084
BoxI GACNNNNGTC 1 cut(s) 759
BpiI GAAGAC 4 cut(s) 659, 788, 1070, 3226
BplI GAGNNNNNCTC 2 cut(s) 2021, 2053
BpmI CTGGAG 4 cut(s) 222, 1069, 2222, 3555
Bpu10I CCTNAGC 3 cut(s) 222, 3687, 3824
Bpu14I TTCGAA 2 cut(s) 185, 3727
BpuEI CTTGAG 5 cut(s) 1769, 2048, 2542, 2929, 3728
BsaAI YACGTR 2 cut(s) 1943, 2840
BsaBI GATNNNNATC 1 cut(s) 1878
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 6 cut(s) 2331, 2979, 3195, 3460, 3579, 3833
BsaWI WCCGGW 3 cut(s) 619, 2407, 3118
Bsc4I CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2880, 2962, 3461
Bse118I RCCGGY 1 cut(s) 2407
Bse1I ACTGG 9 cut(s) 1838, 2205, 2342, 2376, 2566, 3079, 3209, 3411, 3572
Bse3DI GCAATG 1 cut(s) 2973
Bse8I GATNNNNATC 1 cut(s) 1878
BseBI CCWGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
BseDI CCNNGG 6 cut(s) 2331, 2979, 3195, 3460, 3579, 3833
BseGI GGATG 3 cut(s) 406, 2477, 3127
BseJI GATNNNNATC 1 cut(s) 1878
BseLI CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2880, 2962, 3461
BseMI GCAATG 1 cut(s) 2973
BseNI ACTGG 9 cut(s) 1838, 2205, 2342, 2376, 2566, 3079, 3209, 3411, 3572
BseRI GAGGAG 5 cut(s) 490, 580, 2285, 2315, 2693
BseSI GKGCMC 2 cut(s) 1174, 1533
BseXI GCAGC 6 cut(s) 76, 299, 2799, 3656, 3659, 3662
BsgI GTGCAG 1 cut(s) 1155
Bsh1285I CGRYCG 1 cut(s) 2889
BshFI GGCC 5 cut(s) 1046, 1645, 2324, 3459, 3763
BshNI GGYRCC 4 cut(s) 491, 860, 3340, 3618
BshTI ACCGGT 1 cut(s) 2407
BsiEI CGRYCG 1 cut(s) 2889
BsiHKAI GWGCWC 5 cut(s) 1533, 1713, 2307, 3567, 3888
BsiHKCI CYCGRG 1 cut(s) 2675
BsiSI CCGG 3 cut(s) 620, 2408, 3119
BslFI GGGAC 1 cut(s) 3478
BslI CCNNNNNNNGG 7 cut(s) 427, 806, 1560, 2337, 2880, 2962, 3461
BsmAI GTCTC 5 cut(s) 46, 263, 2159, 2670, 3137
BsmFI GGGAC 1 cut(s) 3478
BsmI GAATGC 4 cut(s) 1210, 1521, 2909, 3828
BsnI GGCC 5 cut(s) 1046, 1645, 2324, 3459, 3763
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 2675
Bsp119I TTCGAA 2 cut(s) 185, 3727
Bsp1286I GDGCHC 6 cut(s) 1174, 1533, 1713, 2307, 3567, 3888
Bsp1407I TGTACA 1 cut(s) 2262
Bsp19I CCATGG 1 cut(s) 2331
BspACI CCGC 5 cut(s) 527, 633, 1751, 2231, 3616
BspANI GGCC 5 cut(s) 1046, 1645, 2324, 3459, 3763
BspHI TCATGA 2 cut(s) 1926, 2530
BspLI GGNNCC 8 cut(s) 493, 862, 1749, 3342, 3466, 3467, 3620, 3820
BspMAI CTGCAG 2 cut(s) 859, 1682
BspMI ACCTGC 3 cut(s) 178, 848, 1793
BspQI GCTCTTC 1 cut(s) 420
BspT104I TTCGAA 2 cut(s) 185, 3727
BspT107I GGYRCC 4 cut(s) 491, 860, 3340, 3618
BspTI CTTAAG 1 cut(s) 2828
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 2973
BsrFI RCCGGY 1 cut(s) 2407
BsrGI TGTACA 1 cut(s) 2262
BsrI ACTGG 9 cut(s) 1838, 2205, 2342, 2376, 2566, 3079, 3209, 3411, 3572
BssAI RCCGGY 1 cut(s) 2407
BssECI CCNNGG 6 cut(s) 2331, 2979, 3195, 3460, 3579, 3833
BssNAI GTATAC 1 cut(s) 714
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 5 cut(s) 2331, 2979, 3195, 3460, 3833
Bst1107I GTATAC 1 cut(s) 714
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
Bst6I CTCTTC 6 cut(s) 261, 420, 2067, 2207, 2961, 3405
BstAFI CTTAAG 1 cut(s) 2828
BstAPI GCANNNNNTGC 1 cut(s) 3340
BstAUI TGTACA 1 cut(s) 2262
BstBAI YACGTR 2 cut(s) 1943, 2840
BstBI TTCGAA 2 cut(s) 185, 3727
BstC8I GCNNGC 8 cut(s) 219, 292, 471, 496, 1231, 1307, 1311, 2432
BstDSI CCRYGG 1 cut(s) 2331
BstEII GGTNACC 1 cut(s) 2422
BstENI CCTNNNNNAGG 1 cut(s) 3459
BstF5I GGATG 3 cut(s) 406, 2477, 3127
BstHHI GCGC 1 cut(s) 406
BstMAI GTCTC 5 cut(s) 46, 263, 2159, 2670, 3137
BstMCI CGRYCG 1 cut(s) 2889
BstMWI GCNNNNNNNGC 7 cut(s) 179, 284, 410, 1708, 1825, 3340, 3650
BstNI CCWGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
BstNSI RCATGY 2 cut(s) 294, 3682
BstPAI GACNNNNGTC 1 cut(s) 759
BstPI GGTNACC 1 cut(s) 2422
BstSCI CCNGG 8 cut(s) 199, 483, 1046, 1246, 2041, 2754, 3454, 3578
BstSFI CTRYAG 7 cut(s) 174, 273, 399, 537, 855, 1678, 2466
BstSLI GKGCMC 2 cut(s) 1174, 1533
BstV1I GCAGC 6 cut(s) 76, 299, 2799, 3656, 3659, 3662
BstV2I GAAGAC 4 cut(s) 659, 788, 1070, 3226
BstX2I RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2822, 3414, 3712, 3818
BstXI CCANNNNNNTGG 1 cut(s) 2332
BstYI RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2822, 3414, 3712, 3818
BstZ17I GTATAC 1 cut(s) 714
BsuI GTATCC 2 cut(s) 2343, 3603
BsuRI GGCC 5 cut(s) 1046, 1645, 2324, 3459, 3763
BtgI CCRYGG 1 cut(s) 2331
BtsCI GGATG 3 cut(s) 406, 2477, 3127
BtsI GCAGTG 1 cut(s) 1172
BveI ACCTGC 3 cut(s) 178, 848, 1793
Cac8I GCNNGC 8 cut(s) 219, 292, 471, 496, 1231, 1307, 1311, 2432
CaiI CAGNNNCTG 1 cut(s) 130
CciI TCATGA 2 cut(s) 1926, 2530
CfoI GCGC 1 cut(s) 406
Cfr10I RCCGGY 1 cut(s) 2407
Cfr13I GGNCC 2 cut(s) 2322, 3465
CsiI ACCWGGT 1 cut(s) 2041
Csp6I GTAC 5 cut(s) 88, 2263, 2559, 3176, 3704
CspAI ACCGGT 1 cut(s) 2407
CviQI GTAC 5 cut(s) 88, 2263, 2559, 3176, 3704
DraI TTTAAA 2 cut(s) 1408, 1978
DraIII CACNNNGTG 2 cut(s) 1618, 2045
DriI GACNNNNNGTC 1 cut(s) 2892
EaeI YGGCCR 3 cut(s) 1643, 3457, 3761
Eam1104I CTCTTC 6 cut(s) 261, 420, 2067, 2207, 2961, 3405
Eam1105I GACNNNNNGTC 1 cut(s) 2892
EarI CTCTTC 6 cut(s) 261, 420, 2067, 2207, 2961, 3405
Ecl136II GAGCTC 2 cut(s) 1711, 3886
Eco130I CCWWGG 5 cut(s) 2331, 2979, 3195, 3460, 3833
Eco147I AGGCCT 1 cut(s) 1046
Eco24I GRGCYC 2 cut(s) 1713, 3888
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 3465
Eco53kI GAGCTC 2 cut(s) 1711, 3886
Eco57I CTGAAG 3 cut(s) 1449, 1578, 2810
Eco88I CYCGRG 1 cut(s) 2675
Eco91I GGTNACC 1 cut(s) 2422
EcoICRI GAGCTC 2 cut(s) 1711, 3886
EcoNI CCTNNNNNAGG 1 cut(s) 3459
EcoO109I RGGNCCY 1 cut(s) 3465
EcoO65I GGTNACC 1 cut(s) 2422
EcoRII CCWGG 8 cut(s) 199, 483, 1046, 1246, 2041, 2754, 3454, 3578
EcoT14I CCWWGG 5 cut(s) 2331, 2979, 3195, 3460, 3833
EcoT22I ATGCAT 2 cut(s) 1521, 1730
EcoT38I GRGCYC 2 cut(s) 1713, 3888
ErhI CCWWGG 5 cut(s) 2331, 2979, 3195, 3460, 3833
FalI AAGNNNNNCTT 4 cut(s) 723, 755, 762, 794
FaqI GGGAC 1 cut(s) 3478
FauNDI CATATG 3 cut(s) 629, 3351, 3383
FbaI TGATCA 1 cut(s) 940
FblI GTMKAC 3 cut(s) 228, 713, 2860
Fnu4HI GCNGC 9 cut(s) 65, 288, 527, 634, 1751, 2813, 3645, 3648, 3651
FokI GGATG 3 cut(s) 393, 2484, 3134
FriOI GRGCYC 2 cut(s) 1713, 3888
Fsp4HI GCNGC 9 cut(s) 65, 288, 527, 634, 1751, 2813, 3645, 3648, 3651
FspBI CTAG 8 cut(s) 17, 791, 1256, 1445, 2069, 2120, 2252, 2921
GlaI GCGC 1 cut(s) 405
GluI GCNGC 9 cut(s) 65, 288, 527, 634, 1751, 2813, 3645, 3648, 3651
GsuI CTGGAG 4 cut(s) 222, 1069, 2222, 3555
HaeIII GGCC 5 cut(s) 1046, 1645, 2324, 3459, 3763
HapII CCGG 3 cut(s) 620, 2408, 3119
HhaI GCGC 1 cut(s) 406
Hin6I GCGC 1 cut(s) 404
HinP1I GCGC 1 cut(s) 404
HincII GTYRAC 3 cut(s) 679, 1566, 3048
HindII GTYRAC 3 cut(s) 679, 1566, 3048
HindIII AAGCTT 4 cut(s) 180, 956, 2444, 3730
HpaI GTTAAC 1 cut(s) 679
HpaII CCGG 3 cut(s) 620, 2408, 3119
HphI GGTGA 8 cut(s) 103, 175, 1193, 2031, 2819, 3267, 3426, 3878
Hpy99I CGWCG 1 cut(s) 2962
HpyCH4IV ACGT 3 cut(s) 841, 1942, 2839
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 284, 410, 1708, 1825, 3340, 3650
HpySE526I ACGT 3 cut(s) 841, 1942, 2839
HspAI GCGC 1 cut(s) 404
KflI GGGWCCC 1 cut(s) 3465
Ksp22I TGATCA 1 cut(s) 940
KspAI GTTAAC 1 cut(s) 679
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 7 cut(s) 503, 1050, 1708, 1747, 2302, 2479, 3883
Lsp1109I GCAGC 6 cut(s) 76, 299, 2799, 3656, 3659, 3662
LweI GCATC 7 cut(s) 115, 415, 811, 1715, 2530, 3398, 3850
MabI ACCWGGT 1 cut(s) 2041
MaeI CTAG 8 cut(s) 17, 791, 1256, 1445, 2069, 2120, 2252, 2921
MaeII ACGT 3 cut(s) 841, 1942, 2839
MaeIII GTNAC 4 cut(s) 964, 2422, 3320, 3545
MfeI CAATTG 3 cut(s) 1146, 2140, 2153
MflI RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2822, 3414, 3712, 3818
MhlI GDGCHC 6 cut(s) 1174, 1533, 1713, 2307, 3567, 3888
MlsI TGGCCA 3 cut(s) 1645, 3459, 3763
MluNI TGGCCA 3 cut(s) 1645, 3459, 3763
MlyI GAGTC 3 cut(s) 1561, 2919, 3043
MmeI TCCRAC 4 cut(s) 1144, 1467, 2943, 3087
Mox20I TGGCCA 3 cut(s) 1645, 3459, 3763
Mph1103I ATGCAT 2 cut(s) 1521, 1730
MroXI GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3733
MscI TGGCCA 3 cut(s) 1645, 3459, 3763
MslI CAYNNNNRTG 4 cut(s) 161, 1266, 2739, 3165
Msp20I TGGCCA 3 cut(s) 1645, 3459, 3763
MspA1I CMGCKG 1 cut(s) 3691
MspCI CTTAAG 1 cut(s) 2828
MspI CCGG 3 cut(s) 620, 2408, 3119
MspR9I CCNGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
MunI CAATTG 3 cut(s) 1146, 2140, 2153
Mva1269I GAATGC 4 cut(s) 1210, 1521, 2909, 3828
MvaI CCWGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
MwoI GCNNNNNNNGC 7 cut(s) 179, 284, 410, 1708, 1825, 3340, 3650
NcoI CCATGG 1 cut(s) 2331
NdeI CATATG 3 cut(s) 629, 3351, 3383
NlaIV GGNNCC 8 cut(s) 493, 862, 1749, 3342, 3466, 3467, 3620, 3820
NsiI ATGCAT 2 cut(s) 1521, 1730
NspI RCATGY 2 cut(s) 294, 3682
NspV TTCGAA 2 cut(s) 185, 3727
PaeI GCATGC 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 2675
PagI TCATGA 2 cut(s) 1926, 2530
PaqCI CACCTGC 1 cut(s) 848
PceI AGGCCT 1 cut(s) 1046
PciI ACATGT 1 cut(s) 3678
PciSI GCTCTTC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 569
PctI GAATGC 4 cut(s) 1210, 1521, 2909, 3828
PdmI GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3733
PflMI CCANNNNNTGG 1 cut(s) 2337
PfoI TCCNGGA 4 cut(s) 199, 483, 1246, 2754
PinAI ACCGGT 1 cut(s) 2407
PkrI GCNGC 9 cut(s) 66, 289, 528, 635, 1752, 2814, 3646, 3649, 3652
PleI GAGTC 3 cut(s) 1561, 2919, 3043
PpsI GAGTC 3 cut(s) 1561, 2919, 3043
Ppu21I YACGTR 2 cut(s) 1943, 2840
PpuMI RGGWCCY 1 cut(s) 3465
PscI ACATGT 1 cut(s) 3678
PshAI GACNNNNGTC 1 cut(s) 759
Psp124BI GAGCTC 2 cut(s) 1713, 3888
Psp5II RGGWCCY 1 cut(s) 3465
Psp6I CCWGG 8 cut(s) 199, 483, 1046, 1246, 2041, 2754, 3454, 3578
PspEI GGTNACC 1 cut(s) 2422
PspGI CCWGG 8 cut(s) 199, 483, 1046, 1246, 2041, 2754, 3454, 3578
PspN4I GGNNCC 8 cut(s) 493, 862, 1749, 3342, 3466, 3467, 3620, 3820
PspPI GGNCC 2 cut(s) 2322, 3465
PspPPI RGGWCCY 1 cut(s) 3465
PspXI VCTCGAGB 1 cut(s) 2675
PsrI GAACNNNNNNTAC 2 cut(s) 3594, 3626
PstI CTGCAG 2 cut(s) 859, 1682
PstNI CAGNNNCTG 1 cut(s) 130
PsuI RGATCY 9 cut(s) 388, 1401, 1780, 1946, 2460, 2822, 3414, 3712, 3818
PvuII CAGCTG 1 cut(s) 3691
RsaI GTAC 5 cut(s) 89, 2264, 2560, 3177, 3705
RsaNI GTAC 5 cut(s) 88, 2263, 2559, 3176, 3704
RseI CAYNNNNRTG 4 cut(s) 161, 1266, 2739, 3165
SacI GAGCTC 2 cut(s) 1713, 3888
SapI GCTCTTC 1 cut(s) 420
SatI GCNGC 9 cut(s) 65, 288, 527, 634, 1751, 2813, 3645, 3648, 3651
Sau96I GGNCC 2 cut(s) 2322, 3465
ScaI AGTACT 1 cut(s) 2560
SchI GAGTC 3 cut(s) 1561, 2919, 3043
ScrFI CCNGG 8 cut(s) 201, 485, 1048, 1248, 2043, 2756, 3456, 3580
SduI GDGCHC 6 cut(s) 1174, 1533, 1713, 2307, 3567, 3888
SexAI ACCWGGT 1 cut(s) 2041
SfaNI GCATC 7 cut(s) 115, 415, 811, 1715, 2530, 3398, 3850
SfcI CTRYAG 7 cut(s) 174, 273, 399, 537, 855, 1678, 2466
Sfr274I CTCGAG 1 cut(s) 2675
SfuI TTCGAA 2 cut(s) 185, 3727
SinI GGWCC 1 cut(s) 3465
SlaI CTCGAG 1 cut(s) 2675
SmiMI CAYNNNNRTG 4 cut(s) 161, 1266, 2739, 3165
SmlI CTYRAG 7 cut(s) 1784, 2027, 2521, 2675, 2828, 2908, 3707
SmoI CTYRAG 7 cut(s) 1784, 2027, 2521, 2675, 2828, 2908, 3707
SphI GCATGC 1 cut(s) 294
SseBI AGGCCT 1 cut(s) 1046
SsiI CCGC 5 cut(s) 527, 633, 1751, 2231, 3616
SspI AATATT 5 cut(s) 449, 550, 882, 1373, 2917
SspMI CTAG 8 cut(s) 17, 791, 1256, 1445, 2069, 2120, 2252, 2921
SstI GAGCTC 2 cut(s) 1713, 3888
StuI AGGCCT 1 cut(s) 1046
StyD4I CCNGG 8 cut(s) 199, 483, 1046, 1246, 2041, 2754, 3454, 3578
StyI CCWWGG 5 cut(s) 2331, 2979, 3195, 3460, 3833
TaiI ACGT 3 cut(s) 844, 1945, 2842
TaqI TCGA 7 cut(s) 185, 563, 572, 896, 2012, 2676, 3727
TaqII GACCGA 1 cut(s) 2903
TatI WGTACW 3 cut(s) 87, 2262, 2558
TauI GCSGC 3 cut(s) 529, 636, 1753
TseI GCWGC 6 cut(s) 64, 287, 2812, 3644, 3647, 3650
TspGWI ACGGA 1 cut(s) 3163
Van91I CCANNNNNTGG 1 cut(s) 2337
Vha464I CTTAAG 1 cut(s) 2828
VneI GTGCAC 1 cut(s) 1529
VpaK11BI GGWCC 1 cut(s) 3465
XagI CCTNNNNNAGG 1 cut(s) 3459
XapI RAATTY 7 cut(s) 431, 519, 1012, 1280, 1981, 2281, 2614
XbaI TCTAGA 1 cut(s) 1444
XceI RCATGY 2 cut(s) 294, 3682
XcmI CCANNNNNNNNNTGG 1 cut(s) 915
XhoI CTCGAG 1 cut(s) 2675
XmiI GTMKAC 3 cut(s) 228, 713, 2860
XmnI GAANNNNTTC 5 cut(s) 257, 1242, 2216, 2397, 3733
XspI CTAG 8 cut(s) 17, 791, 1256, 1445, 2069, 2120, 2252, 2921
ZrmI AGTACT 1 cut(s) 2560
Zsp2I ATGCAT 2 cut(s) 1521, 1730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.