FvH4_2g36810

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000203
Physical Location & Seq
Forward (+)
26895790 .. 26899733
3944 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36810.t1

Sequence Viewer

Length: 3867 bp
ATGGCACTGAAAGAAATGGCAGAGACAGCTATTACAGAAGGTGCTGTGCCGGTTGCAAATTGGACATTGAAAAAACTTTGGCAAGAGCTGGGTTATATGTGTTGTTACAAAAGCAACATTGGAGATCTCAACGAGCAGGTTCGGCTGCTGCATGACAAAAGAGCTGAAATAAAGCTGGAGGAGGAAACTGCAAGAGAAGATTCAAAGATTATTAATGGCCAAGTTATTGATTGGCTGGCCAAGGCAGACGAGCAACTAAAAGAAAAGGAGACGACTTTCAGTGAAGAAGCTGTAGCAGCAAAGGCAGCATGCTGTAATGGATGGCTTCCAAATATGAAGGGCCGTTTTTATCTGGCAAGGAATGCTAAGAAGATGACTCTGGCGGTTGATCAGCTCCTTACTAAAGATATCGGGATTATAGCGCATCCTGCTCCTCACCCAGAAGTGGAATTTCAACCCACAGAGGACCCTAATCAGCTGGAAACCAACTTCCATGAAGGTGCCAGCTCCTCAGGAACATCAAACGATGAACAGCCACCTGCATCTGAGAGGAAAAATATTTTTTTTGATTCAAGAAGATCAACAGTTGGCGAAGTGATGGAAGCTTTGAGGGGGAATCAAATCAATCCGATTATCATATGCGGCATGGGCGGCATTGGTAAAACAACATTGATGGGGCAAGTTCTTGAGAAAGCAAAGAAAGAGGGTCTGTTCGATGAGTATACAAAGGCAACCGTTAAGGAGTCTCTTAACAAAGCTGCTGACGTGATTCAGATTCAAGCTGAACTTGCAGAATATTTAGGCACGAAGCTCCTTGAGGACGTAAAAGAGAGCGGTCCAAGAGCAAGTAAGCTACGTGAAAGATTATCTAAAGGCAGCAAGAAGATCCTTGTAATGTTAGACAATGTTTCGACAACAACCCCAGACTTTTTGTGGGACATAGGGATTCCCCTTAGTTGTCAAATTTTGGTAACATCAAGACAGCAAGATCTTTTCATGGACATGGACACAAGAAAGAATTTCCCGATTCATGGTTTGCCGGCTGGAGATGCCTGGAGTCTGTTCAAGAAGACAGCAGGAAGTTCCATCGAATCTGATCCCGAGTTGCATGTTCAAGCGGAAAAGGTTCTGAAGGAATGTGGTGGGTTGCCGATTGCAATTTCGACGGTTGGAACGGCACTTAAAGGTCAAAGCATTGGAATTTGGACGAATGCACTGAGGGAACTAGAAAAGGCTTGCCCAGAAAACGTTCCAGGAGTGATAGAACATGTGTATGGGAAAATCAAGTTCAGCTATGAATGTTTGCCAAATGAGCAAGCCAAATCATGCTTGTTGCTGTGTTGCATATTTGAAGAATGTAGTGATATACTGATTGAAGATTTGGTAATATATGGGCATGGGCTTGGGCTCCTTAGAGGCATTGATTCAATACTTGAAGGAAGGAATCGTGTGGAAACATTGGTTCACACACTCAAAAGTCGCTTTCTATTGTTAGAAAGCAATAATAAAGAGACAGTGAAAATGCACGATGTGGTGCGTGATGTTGCCATACATATAGCCTCTGAAGAGTTTGTAGAAAAGAAAAGTGATGGGGTTGAAAAGAGAATAGTTGTGAGAGACAAAGCAGCAACTGTGAAGTTGATGGATTGGCCTCAGGATAGTGATTCTGAGAAATCTACTTATTGCAGTTCTCTGAGTATCAGTGGGTTTTGTCAGCTTTTACTGTTGAGACAAATAACTAGGTTTCCAATGGAGCCGCCACCCACAATTTTCAAAAGAACGGAAGATCTCATGGTTCTAACAATCAGTGGATATGATAGATGGACGACTCTGACTTTGTCAGTAGACTCGGTACTACCATCGCTTGCAGTAATGAGAAACCTTCAAACACTGAGGGTAGAGAGGTGCGGATTCAACTGTGATGTATCTGTCATTGGGGAGCTAAAGACACTCATGATACTCAGTCTACGTGGATGTCGGAGACTTGAGCAATTGCCTGATACATTTAAAAACTTGTCTGATCTAAGACTGCTTGATTTGACAGATTGTTACAAGCTTGAAGTAATCTCGCCAGGTGTGATATCAAGTTTAACAAGACTAGAAGAATTGTACATGTTGGATGGCTTTAAAAATTGGGAGGTTGAGAAACTAGCCTCCACCGAAACAACCGAGTGGATCTCTCAATTGGAGGAGGCTGAGCAGGAAAGAGCCGCTGCTGGATCCCAAGACGCGGAAACTACACTTTACACGTGGAAGGGACTCAATGAATTGGCGGTTGTGCAACCAGCAGAGGTAGGAAAGCAGTTTAGAGAAGAGGTTCAAAAACCTCGGAAACAAAACGAGGATCATACAAGGTGGGCCAACTCGATGGACTGGAACACTGGGATCCTTGCAGAACTGCTCTCCTTGTCCCGTTTGACTACCTTAGAAGTTGCTCTACCACCTCTCAATATACTTCTAACCACTCCTCTCTTTAACAAGCTCGAAAGATTTAAGATCTCTATAGGGTGGAAGGAGTATAGTGGTCTCACTTATGAGGAAATAGAGGAGGAGCAGAGTGGCAACTACTTGAGAGTTCACCATCTTGATGTAAGCAACGCTGCAATGGGTAGTGGAGTCACTTCGTTGCTAGAGAAAGCCAGCTATGTTGAGTTTAAATTGACTCAGCCTCAACTTCTGAATGTCTTGGATAGAGTCTGTTTCCCAAAGACAAAGCGTCTTAAGTTCCAGGAATGTAATGATGTGGAGTATCTGATTGACACGACTACTACTACACATAGCAGCAGCGCCATATTTCCTGTCTTGAATTCACTGGAGATTAGTAAGGCAAGGAGGCTGAAAGTGATTTTTCATGGTGAACTGCCAACGGGGTTTCTCAAGCAACTACGTGCATTGATATTATTCAACCTACCAGCATTGACCTATATTTGGAAGACCAACAGTCGATCTCAGTATGATAATGAGGAAGTGATCAACTTCCAAAGTCTGACAAAATTGGATCTCTCAGATTTACCAAGCTTTACTAGCATAACCAAATATGCCTCTAAGGGGACGGATAGGCTACCAGAAGACAGCAGCCCGGAACAATCTGACATCACTATACTATCTCTGTTCGACACCAAGGTTCATTTCCCAGTCCTGACAGAACTACGCTTGGGCTCCATGAACTTCAAAGAGATATGGAACAACCAACTTTCAGCAGAATCCTTTTGTGAGCTAAGAATTCTACGGGTAGAGCGATGTCACGAACTGTTGCATTTGGTACCAACACATATGCAAAATAGATTGCAGAAATTGGAGTACATAGAAGCTTGGCGCTGTTCATCACTTAAAGAGATATTTGAATTTAGAAGATTGATTGTTGATGATGAAGGGGATGCTACAACAATATCTGAGTCAGGAGATCAAGGGGCGCAAATAAACAAGATGATGTCTTTCAAACAATCTCGTCAAGCCTTTCAGAATCTTAGAGATATGTCAATTTGGCGTTGTGAAAGTTTGAGAACTCTGCTCTCTCCCTCAGTTGCTAGAGGTCTCGTGAAGCTTCAAACGCTAAAGATAGAAAAGTGTAGGAAGATTGAAGAGATAGTAGCAGCACCAACAGAGGGTGAAGAAACAGAGGATGACAACATGTTCCCTCAGCTATGTACTCTGGAACTTATGGTTCTACCAAATCTCAGAAGCTTTTCACAAGGCAAATATAATTTCAGATGGCCACTGGTGAAAGACATCACAATCCTCCACTGCTACAATATGAAGATGTTTTGTTTAGGATCCCTCAGCACACCGAAGGGAGTGAAAATAGATGTCCAATGTGCTGGTGAGAGTGTTTTGCAGGAGCTCAATGACAGTAGACAAGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1289

Amino Acids

145.64

Weight (kDa)

5.41

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 194 - 360 3.2e-19 NB-ARC domain
LRR_14 PF23598 618 - 707 3.6e-06 Leucine-rich repeat region
LRR_8 PF13855 650 - 705 5.3e-06 Leucine rich repeat
LRR_RPS2 PF23247 897 - 1010 3e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1048 - 1191 1.4e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 547
Acc36I ACCTGC 2 cut(s) 127, 547
Acc65I GGTACC 1 cut(s) 3262
AccB1I GGYRCC 2 cut(s) 500, 3262
AccBSI CCGCTC 1 cut(s) 834
AccI GTMKAC 4 cut(s) 722, 1845, 1966, 3853
AccII CGCG 1 cut(s) 2231
AclI AACGTT 1 cut(s) 1248
AcoI YGGCCR 3 cut(s) 217, 237, 3713
AcsI RAATTY 7 cut(s) 449, 963, 1018, 1200, 2806, 3222, 3344
AcuI CTGAAG 2 cut(s) 1151, 1584
AcvI CACGTG 1 cut(s) 2250
AdeI CACNNNGTG 1 cut(s) 1531
AfaI GTAC 5 cut(s) 1854, 2111, 3264, 3302, 3649
AfiI CCNNNNNNNGG 6 cut(s) 445, 1031, 2230, 2928, 3048, 3605
AflII CTTAAG 1 cut(s) 2720
AflIII ACRYGT 4 cut(s) 1267, 2112, 2247, 3630
AhdI GACNNNNNGTC 2 cut(s) 2715, 2940
AjiI CACGTC 1 cut(s) 766
AjnI CCWGG 4 cut(s) 1052, 1252, 2071, 2727
AjuI GAANNNNNNNTTGG 2 cut(s) 3113, 3145
Alw21I GWGCWC 1 cut(s) 3843
Alw26I GTCTC 9 cut(s) 17, 263, 750, 1505, 1611, 1723, 1975, 2531, 3539
AlwNI CAGNNNCTG 1 cut(s) 1631
Ama87I CYCGRG 1 cut(s) 1100
AoxI GGCC 6 cut(s) 217, 237, 340, 1649, 2358, 3713
ApoI RAATTY 7 cut(s) 449, 963, 1018, 1200, 2806, 3222, 3344
ArsI GACNNNNNNTTYG 2 cut(s) 1819, 1851
AseI ATTAAT 1 cut(s) 213
Asp700I GAANNNNTTC 4 cut(s) 1125, 1248, 2316, 3685
Asp718I GGTACC 1 cut(s) 3262
AspLEI GCGC 4 cut(s) 424, 2789, 3318, 3415
AspS9I GGNCC 4 cut(s) 340, 466, 836, 2358
AsuC2I CCSGG 1 cut(s) 3080
AsuHPI GGTGA 6 cut(s) 428, 2570, 2867, 3620, 3733, 3833
AvaI CYCGRG 1 cut(s) 1100
AvaII GGWCC 2 cut(s) 466, 836
AxyI CCTNAGG 2 cut(s) 511, 1653
BalI TGGCCA 3 cut(s) 219, 239, 3715
BamHI GGATCC 3 cut(s) 2219, 2385, 3773
BanI GGYRCC 2 cut(s) 500, 3262
BanII GRGCYC 3 cut(s) 1410, 3161, 3843
BarI GAAGNNNNNNTAC 2 cut(s) 2421, 2453
BauI CACGAG 1 cut(s) 3536
BbrPI CACGTG 1 cut(s) 2250
BbsI GAAGAC 3 cut(s) 1076, 2939, 3075
Bbv12I GWGCWC 1 cut(s) 3843
BbvCI CCTCAGC 2 cut(s) 3639, 3779
BceAI ACGGC 2 cut(s) 327, 1191
BciT130I CCWGG 4 cut(s) 1054, 1254, 2073, 2729
BclI TGATCA 2 cut(s) 388, 2970
BcnI CCSGG 1 cut(s) 3080
BcoDI GTCTC 9 cut(s) 17, 263, 750, 1505, 1611, 1723, 1975, 2531, 3539
BfaI CTAG 7 cut(s) 1226, 1740, 2099, 2150, 2630, 3024, 3528
BfmI CTRYAG 2 cut(s) 291, 2502
BfoI RGCGCY 2 cut(s) 2790, 3319
BfrI CTTAAG 1 cut(s) 2720
BfuAI ACCTGC 2 cut(s) 127, 547
BglII AGATCT 4 cut(s) 124, 988, 1786, 2496
BlpI GCTNAGC 1 cut(s) 2196
Bme1390I CCNGG 5 cut(s) 1054, 1254, 2073, 2729, 3080
Bme18I GGWCC 2 cut(s) 466, 836
BmeRI GACNNNNNGTC 2 cut(s) 2715, 2940
BmeT110I CYCGRG 1 cut(s) 1100
BmgBI CACGTC 1 cut(s) 766
BmgT120I GGNCC 4 cut(s) 340, 466, 836, 2358
BmiI GGNNCC 9 cut(s) 468, 502, 1409, 1755, 2221, 2387, 3160, 3264, 3775
BmrFI CCNGG 5 cut(s) 1054, 1254, 2073, 2729, 3080
BmrI ACTGGG 2 cut(s) 2391, 3128
BmsI GCATC 4 cut(s) 433, 551, 1039, 3367
BmuI ACTGGG 2 cut(s) 2391, 3128
BpiI GAAGAC 3 cut(s) 1076, 2939, 3075
BplI GAGNNNNNCTC 2 cut(s) 2162, 2194
BpmI CTGGAG 4 cut(s) 197, 1065, 1075, 2834
Bpu10I CCTNAGC 2 cut(s) 3639, 3779
Bpu1102I GCTNAGC 1 cut(s) 2196
BpuEI CTTGAG 5 cut(s) 707, 836, 2006, 2590, 2861
BpuMI CCSGG 1 cut(s) 3080
BsaAI YACGTR 4 cut(s) 857, 1970, 2250, 2888
BsaI GGTCTC 2 cut(s) 2531, 3539
BsaJI CCNNGG 3 cut(s) 240, 2327, 3120
Bsc4I CCNNNNNNNGG 6 cut(s) 445, 1031, 2230, 2928, 3048, 3605
Bse118I RCCGGY 2 cut(s) 49, 1039
Bse1I ACTGG 5 cut(s) 2378, 2386, 2817, 3134, 3723
Bse21I CCTNAGG 2 cut(s) 511, 1653
Bse3DI GCAATG 1 cut(s) 2610
BseBI CCWGG 4 cut(s) 1054, 1254, 2073, 2729
BseDI CCNNGG 3 cut(s) 240, 2327, 3120
BseGI GGATG 6 cut(s) 326, 424, 1979, 2125, 3382, 3628
BseLI CCNNNNNNNGG 6 cut(s) 445, 1031, 2230, 2928, 3048, 3605
BseMI GCAATG 1 cut(s) 2610
BseNI ACTGG 5 cut(s) 2378, 2386, 2817, 3134, 3723
BseRI GAGGAG 7 cut(s) 194, 423, 499, 2204, 2457, 2561, 2564
BseYI CCCAGC 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 2231
BshFI GGCC 6 cut(s) 219, 239, 342, 1651, 2360, 3715
BshNI GGYRCC 2 cut(s) 500, 3262
BsiHKAI GWGCWC 1 cut(s) 3843
BsiHKCI CYCGRG 1 cut(s) 1100
BsiSI CCGG 3 cut(s) 50, 1040, 3080
BslFI GGGAC 4 cut(s) 950, 2271, 2395, 3064
BslI CCNNNNNNNGG 6 cut(s) 445, 1031, 2230, 2928, 3048, 3605
BsmAI GTCTC 9 cut(s) 17, 263, 750, 1505, 1611, 1723, 1975, 2531, 3539
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 4 cut(s) 950, 2271, 2395, 3064
BsmI GAATGC 2 cut(s) 367, 1216
BsnI GGCC 6 cut(s) 219, 239, 342, 1651, 2360, 3715
Bso31I GGTCTC 2 cut(s) 2531, 3539
BsoBI CYCGRG 1 cut(s) 1100
Bsp1286I GDGCHC 3 cut(s) 1410, 3161, 3843
Bsp1407I TGTACA 1 cut(s) 2109
Bsp1720I GCTNAGC 1 cut(s) 2196
BspANI GGCC 6 cut(s) 219, 239, 342, 1651, 2360, 3715
BspFNI CGCG 1 cut(s) 2231
BspHI TCATGA 1 cut(s) 1953
BspLI GGNNCC 9 cut(s) 468, 502, 1409, 1755, 2221, 2387, 3160, 3264, 3775
BspMI ACCTGC 2 cut(s) 127, 547
BspT107I GGYRCC 2 cut(s) 500, 3262
BspTI CTTAAG 1 cut(s) 2720
BspTNI GGTCTC 2 cut(s) 2531, 3539
BsrBI CCGCTC 1 cut(s) 834
BsrDI GCAATG 1 cut(s) 2610
BsrFI RCCGGY 2 cut(s) 49, 1039
BsrGI TGTACA 1 cut(s) 2109
BsrI ACTGG 5 cut(s) 2378, 2386, 2817, 3134, 3723
BssAI RCCGGY 2 cut(s) 49, 1039
BssECI CCNNGG 3 cut(s) 240, 2327, 3120
BssNAI GTATAC 1 cut(s) 723
BssSI CACGAG 1 cut(s) 3536
BssT1I CCWWGG 2 cut(s) 240, 3120
Bst1107I GTATAC 1 cut(s) 723
Bst2BI CACGAG 1 cut(s) 3536
Bst2UI CCWGG 4 cut(s) 1054, 1254, 2073, 2729
Bst6I CTCTTC 3 cut(s) 1560, 2307, 3576
BstAFI CTTAAG 1 cut(s) 2720
BstAPI GCANNNNNTGC 1 cut(s) 362
BstAUI TGTACA 1 cut(s) 2109
BstBAI YACGTR 4 cut(s) 857, 1970, 2250, 2888
BstC8I GCNNGC 8 cut(s) 237, 310, 505, 1041, 1237, 1317, 1866, 2641
BstF5I GGATG 6 cut(s) 326, 424, 1979, 2125, 3382, 3628
BstFNI CGCG 1 cut(s) 2231
BstH2I RGCGCY 2 cut(s) 2790, 3319
BstHHI GCGC 4 cut(s) 424, 2789, 3318, 3415
BstMAI GTCTC 9 cut(s) 17, 263, 750, 1505, 1611, 1723, 1975, 2531, 3539
BstNI CCWGG 4 cut(s) 1054, 1254, 2073, 2729
BstNSI RCATGY 5 cut(s) 312, 1112, 1271, 2116, 3634
BstSCI CCNGG 5 cut(s) 1052, 1252, 2071, 2727, 3078
BstSFI CTRYAG 2 cut(s) 291, 2502
BstUI CGCG 1 cut(s) 2231
BstV2I GAAGAC 3 cut(s) 1076, 2939, 3075
BstXI CCANNNNNNTGG 2 cut(s) 2368, 3818
BstZ17I GTATAC 1 cut(s) 723
Bsu36I CCTNAGG 2 cut(s) 511, 1653
BsuRI GGCC 6 cut(s) 219, 239, 342, 1651, 2360, 3715
BtgZI GCGATG 2 cut(s) 1845, 3253
BtrI CACGTC 1 cut(s) 766
BtsCI GGATG 6 cut(s) 326, 424, 1979, 2125, 3382, 3628
BtsI GCAGTG 1 cut(s) 3742
BveI ACCTGC 2 cut(s) 127, 547
Cac8I GCNNGC 8 cut(s) 237, 310, 505, 1041, 1237, 1317, 1866, 2641
CaiI CAGNNNCTG 1 cut(s) 1631
CciI TCATGA 1 cut(s) 1953
CfoI GCGC 4 cut(s) 424, 2789, 3318, 3415
Cfr10I RCCGGY 2 cut(s) 49, 1039
Cfr13I GGNCC 4 cut(s) 340, 466, 836, 2358
CseI GACGC 2 cut(s) 2237, 2705
Csp6I GTAC 5 cut(s) 1853, 2110, 3263, 3301, 3648
CviQI GTAC 5 cut(s) 1853, 2110, 3263, 3301, 3648
DraI TTTAAA 3 cut(s) 2008, 2128, 2656
DraIII CACNNNGTG 1 cut(s) 1531
DriI GACNNNNNGTC 2 cut(s) 2715, 2940
EaeI YGGCCR 3 cut(s) 217, 237, 3713
Eam1104I CTCTTC 3 cut(s) 1560, 2307, 3576
Eam1105I GACNNNNNGTC 2 cut(s) 2715, 2940
EarI CTCTTC 3 cut(s) 1560, 2307, 3576
Ecl136II GAGCTC 1 cut(s) 3841
Eco130I CCWWGG 2 cut(s) 240, 3120
Eco24I GRGCYC 3 cut(s) 1410, 3161, 3843
Eco31I GGTCTC 2 cut(s) 2531, 3539
Eco32I GATATC 2 cut(s) 409, 2082
Eco47I GGWCC 2 cut(s) 466, 836
Eco53kI GAGCTC 1 cut(s) 3841
Eco57I CTGAAG 2 cut(s) 1151, 1584
Eco72I CACGTG 1 cut(s) 2250
Eco81I CCTNAGG 2 cut(s) 511, 1653
Eco88I CYCGRG 1 cut(s) 1100
EcoICRI GAGCTC 1 cut(s) 3841
EcoO109I RGGNCCY 1 cut(s) 466
EcoRI GAATTC 2 cut(s) 2806, 3222
EcoRII CCWGG 4 cut(s) 1052, 1252, 2071, 2727
EcoRV GATATC 2 cut(s) 409, 2082
EcoT14I CCWWGG 2 cut(s) 240, 3120
EcoT38I GRGCYC 3 cut(s) 1410, 3161, 3843
ErhI CCWWGG 2 cut(s) 240, 3120
Esp3I CGTCTC 1 cut(s) 263
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 4 cut(s) 950, 2271, 2395, 3064
FauNDI CATATG 2 cut(s) 638, 3273
FbaI TGATCA 2 cut(s) 388, 2970
FblI GTMKAC 4 cut(s) 722, 1845, 1966, 3853
FokI GGATG 6 cut(s) 333, 411, 1986, 2132, 3389, 3635
FriOI GRGCYC 3 cut(s) 1410, 3161, 3843
FspBI CTAG 7 cut(s) 1226, 1740, 2099, 2150, 2630, 3024, 3528
GlaI GCGC 4 cut(s) 423, 2788, 3317, 3414
GsaI CCCAGC 1 cut(s) 92
GsuI CTGGAG 4 cut(s) 197, 1065, 1075, 2834
HaeII RGCGCY 2 cut(s) 2790, 3319
HaeIII GGCC 6 cut(s) 219, 239, 342, 1651, 2360, 3715
HapII CCGG 3 cut(s) 50, 1040, 3080
HgaI GACGC 2 cut(s) 2237, 2705
HhaI GCGC 4 cut(s) 424, 2789, 3318, 3415
Hin6I GCGC 4 cut(s) 422, 2787, 3316, 3413
HinP1I GCGC 4 cut(s) 422, 2787, 3316, 3413
HindIII AAGCTT 6 cut(s) 603, 2054, 3016, 3309, 3542, 3682
HpaII CCGG 3 cut(s) 50, 1040, 3080
HphI GGTGA 6 cut(s) 428, 2570, 2867, 3620, 3733, 3833
Hpy166II GTNNAC 7 cut(s) 723, 1465, 1846, 1967, 2578, 2858, 3854
Hpy8I GTNNAC 7 cut(s) 723, 1465, 1846, 1967, 2578, 2858, 3854
Hpy99I CGWCG 1 cut(s) 1168
HpyCH4IV ACGT 7 cut(s) 765, 822, 856, 1248, 1969, 2249, 2887
HpySE526I ACGT 7 cut(s) 765, 822, 856, 1248, 1969, 2249, 2887
HspAI GCGC 4 cut(s) 422, 2787, 3316, 3413
KpnI GGTACC 1 cut(s) 3266
KroI GCCGGC 1 cut(s) 1039
KroNI GCCGGC 1 cut(s) 1041
Ksp22I TGATCA 2 cut(s) 388, 2970
LweI GCATC 4 cut(s) 433, 551, 1039, 3367
MaeI CTAG 7 cut(s) 1226, 1740, 2099, 2150, 2630, 3024, 3528
MaeII ACGT 7 cut(s) 765, 822, 856, 1248, 1969, 2249, 2887
MaeIII GTNAC 5 cut(s) 104, 970, 2048, 2617, 3242
MbiI CCGCTC 1 cut(s) 834
MfeI CAATTG 2 cut(s) 1991, 2183
MhlI GDGCHC 3 cut(s) 1410, 3161, 3843
MlsI TGGCCA 3 cut(s) 219, 239, 3715
MluNI TGGCCA 3 cut(s) 219, 239, 3715
MmeI TCCRAC 3 cut(s) 1150, 1958, 2097
Mox20I TGGCCA 3 cut(s) 219, 239, 3715
MroNI GCCGGC 1 cut(s) 1039
MroXI GAANNNNTTC 4 cut(s) 1125, 1248, 2316, 3685
MscI TGGCCA 3 cut(s) 219, 239, 3715
MslI CAYNNNNRTG 4 cut(s) 498, 1001, 1272, 2586
Msp20I TGGCCA 3 cut(s) 219, 239, 3715
MspA1I CMGCKG 2 cut(s) 478, 2213
MspCI CTTAAG 1 cut(s) 2720
MspI CCGG 3 cut(s) 50, 1040, 3080
MspR9I CCNGG 5 cut(s) 1054, 1254, 2073, 2729, 3080
MunI CAATTG 2 cut(s) 1991, 2183
Mva1269I GAATGC 2 cut(s) 367, 1216
MvaI CCWGG 4 cut(s) 1054, 1254, 2073, 2729
MvnI CGCG 1 cut(s) 2231
NaeI GCCGGC 1 cut(s) 1041
NciI CCSGG 1 cut(s) 3080
NdeI CATATG 2 cut(s) 638, 3273
NgoMIV GCCGGC 1 cut(s) 1039
NlaIV GGNNCC 9 cut(s) 468, 502, 1409, 1755, 2221, 2387, 3160, 3264, 3775
NmuCI GTSAC 2 cut(s) 2617, 3242
NspI RCATGY 5 cut(s) 312, 1112, 1271, 2116, 3634
PaeI GCATGC 1 cut(s) 312
PagI TCATGA 1 cut(s) 1953
PaqCI CACCTGC 1 cut(s) 547
PciI ACATGT 3 cut(s) 1267, 2112, 3630
PcsI WCGNNNNNNNCGW 1 cut(s) 3235
PctI GAATGC 2 cut(s) 367, 1216
PdiI GCCGGC 1 cut(s) 1041
PdmI GAANNNNTTC 4 cut(s) 1125, 1248, 2316, 3685
PflFI GACNNNGTC 1 cut(s) 1837
PfoI TCCNGGA 2 cut(s) 1252, 2727
PmaCI CACGTG 1 cut(s) 2250
PmlI CACGTG 1 cut(s) 2250
Ppu21I YACGTR 4 cut(s) 857, 1970, 2250, 2888
PpuMI RGGWCCY 1 cut(s) 466
PscI ACATGT 3 cut(s) 1267, 2112, 3630
PshBI ATTAAT 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 3843
Psp1406I AACGTT 1 cut(s) 1248
Psp5II RGGWCCY 1 cut(s) 466
Psp6I CCWGG 4 cut(s) 1052, 1252, 2071, 2727
PspCI CACGTG 1 cut(s) 2250
PspFI CCCAGC 1 cut(s) 88
PspGI CCWGG 4 cut(s) 1052, 1252, 2071, 2727
PspN4I GGNNCC 9 cut(s) 468, 502, 1409, 1755, 2221, 2387, 3160, 3264, 3775
PspPI GGNCC 4 cut(s) 340, 466, 836, 2358
PspPPI RGGWCCY 1 cut(s) 466
PstNI CAGNNNCTG 1 cut(s) 1631
PsyI GACNNNGTC 1 cut(s) 1837
PvuII CAGCTG 1 cut(s) 478
RsaI GTAC 5 cut(s) 1854, 2111, 3264, 3302, 3649
RsaNI GTAC 5 cut(s) 1853, 2110, 3263, 3301, 3648
RseI CAYNNNNRTG 4 cut(s) 498, 1001, 1272, 2586
SacI GAGCTC 1 cut(s) 3843
Sau96I GGNCC 4 cut(s) 340, 466, 836, 2358
ScrFI CCNGG 5 cut(s) 1054, 1254, 2073, 2729, 3080
SduI GDGCHC 3 cut(s) 1410, 3161, 3843
SfaNI GCATC 4 cut(s) 433, 551, 1039, 3367
SfcI CTRYAG 2 cut(s) 291, 2502
SinI GGWCC 2 cut(s) 466, 836
SmiMI CAYNNNNRTG 4 cut(s) 498, 1001, 1272, 2586
SmlI CTYRAG 6 cut(s) 686, 815, 1985, 2569, 2720, 2876
SmoI CTYRAG 6 cut(s) 686, 815, 1985, 2569, 2720, 2876
SphI GCATGC 1 cut(s) 312
SspI AATATT 2 cut(s) 559, 797
SspMI CTAG 7 cut(s) 1226, 1740, 2099, 2150, 2630, 3024, 3528
SstI GAGCTC 1 cut(s) 3843
StyD4I CCNGG 5 cut(s) 1052, 1252, 2071, 2727, 3078
StyI CCWWGG 2 cut(s) 240, 3120
TaiI ACGT 7 cut(s) 768, 825, 859, 1251, 1972, 2252, 2890
TaqI TCGA 8 cut(s) 714, 911, 1089, 1163, 2366, 2484, 2944, 3114
TatI WGTACW 3 cut(s) 2109, 3300, 3647
TauI GCSGC 4 cut(s) 645, 654, 1759, 2213
TseFI GTSAC 2 cut(s) 2617, 3242
Tsp45I GTSAC 2 cut(s) 2617, 3242
TspGWI ACGGA 2 cut(s) 1796, 3068
Tth111I GACNNNGTC 1 cut(s) 1837
Vha464I CTTAAG 1 cut(s) 2720
VpaK11BI GGWCC 2 cut(s) 466, 836
VspI ATTAAT 1 cut(s) 213
XapI RAATTY 7 cut(s) 449, 963, 1018, 1200, 2806, 3222, 3344
XceI RCATGY 5 cut(s) 312, 1112, 1271, 2116, 3634
XcmI CCANNNNNNNNNTGG 1 cut(s) 930
XmiI GTMKAC 4 cut(s) 722, 1845, 1966, 3853
XmnI GAANNNNTTC 4 cut(s) 1125, 1248, 2316, 3685
XspI CTAG 7 cut(s) 1226, 1740, 2099, 2150, 2630, 3024, 3528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.