Rw6G038980

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000203
Physical Location & Seq
Reverse (-)
63047236 .. 63056819
9584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G038980.1

Sequence Viewer

Length: 3807 bp
ATGGACATTATTCTAGCATTGGTACAACCATTGGCAGAATGGGCAGTGAGACCAATTCGGCGACAGTTGAGTTATCATCTCAACGAACAGGTTCTGCTGCTGCGTGACAAAAGAGGAGGAGTAAACCTGGACGTGACAGATGCGAGACGAGCTCTACAGGAGATTAGTCCCGGAGTTGATCGCTGGCTCGGTGATGTAGACAAGATCATAGTAGAAGAGGAGACGAGCTTCAGTGAAGAAACTGTAGCAGCAAAGGCAGCGTGCTGTAATGGGTGGTTGCCAAATTTGAAGTGTCGTCATTCTTTGGGAAGGAAAGCTATGAAGATGACAGAGAAGGTGGATAAACTCCTTGCTAATGAACCCAGGGTTATAGCGCATCCTGCTGCTCCCCCAAAGGTGGAATTCCAACCCACAGAGCACTCTACTCTGCTCGGAACCAACTTCCATGAAGGTGCCAGCTCCTCTGCAGGAACATCAACCCAAGAACAGCCACCTGCATCTGAAGGAAATAATATTAATTTTGATTCAAGAAGATCAACCATTAGAGATGTGATGGAGGCTCTCAAGGGGAATCAATTCAATCCGATTATCATATGCGGCATGGGCGGCATCGGGAAGACGACATTGATGGGGCAAGTTTTTGAGAAAGCAAAGGAAGAAGGTCTGTTCGATGAGTATACAAAGGCAACCTTTAAGGAATCTCTTGACAAAGCTCCTGACGTGATTCAGATTCAAGGCGAACTTGCAGAATATCTAGGCAAGAACATCCTTAAGGATGAAAAAGAGAGAGGTCCAAGAGCAAGTAAGCTACGTGCAAGATTATCCCAAGGCAGCAAGAAGATCCTTGTAATGTTAGACAATGTTTCCACAACAACCCCAGACTTTTTGTGGGACATAGGGATTCCCCCTAGTTGTAAACTTTTGGTGACATCAAGACAACAAGATCTATTCAAGGACATGGACACAAGAAAGAATTTCCCCATTCATGGTTTGCCGGATCCTGATGCCTGGAGTCTGTTTAAGAAGACAGCAGGAAGTTCTATCGAATCTGATCGCGAGTTGCAAGTTGTAGCAAAAGAGGTTTTGAAGGAATGTGCTGGGTTGCCCATTGCAATTTCAACTGTTGGAACGGCACTAAAAGGTGAAAACATTGCAGTTTGGAGGAATGCACTAAGGGAACTGAAGAAGGCTAATCCAGAAAACGTTCCAGGAGTGATAGAACATGTGTATGGCAAAATCAAGTTCAGCTATAAATGTTTACCAAGCGAGCAAGCCAAGTCATGCCGCTATATAGAGATAGAAGATTTGGTTAGATATGGGCTCGGTCTCAAGCTCTTTAAAGGCATTGAATCAATGGTTGAAGGAAGAAACTGTATAGAAACATTGGTTCAGACACTCAAATATCGCTTTTTATTGTTAGAGAGCGATGTGCCAGAGCGTGTGAGAATGCATGATGTGGTGCGTGATGTTGCCGTGCATATAGCTTCTGAAGAGTTTGTAGAAAAGAAAAATGATAGAATTGAAAAGAGAATTGTGGTGAGAGACAGAGCAGAAACTGTGAAGTTGATGGATTGGCCTAAGGATAGTGGTTCTGAGCAACCTATTTATTGCAGTTCACTGAGTATCAATGGGCTTTGGCAACTTTTATTGTTGAGACATGAAGTTGGACCTCCATTGAAGCCTCCACCCATAATTTTTAATAGAATGGAAGATCTCAAGGTTCTAGCAATTAGGAGGATGTCTCTGTTATCACTAGTCCCATCACTTACAGTTTTAAAAAACCTTCAAACACTCTGTCTAGAGAGCTGCGATCTGGTGTTTGATGTATCAGTCATTGGGCAGCTAAGGACACTCATGATACTCAGTTTGCGTGAATCCAAAATCAGGGAATTGCCTGATACATTTAAAAATTTGTCTAATCTGAGATTGCTTGATTTGTTCGAATGTGAGGATGTTGTAATAATCCCACCAGGTGTCATATCAAGATTATCCGGATTAGAAGAATTGCACATGTGGAACAGCTTTGAGAATTGGGTTGTTGATAAACTAACCTCAATCGAAACAACCGATTGGATCTCTCAATTAGAGGCTGAGGAAAGAGTTGGACCCGAAGAAGTGGACGAGTGGAAGAGAATCAACACATTGGCAGCTGAACAACCAGCCAAGCAAGAGGAGATTATTTGGCTTTTCTTCAAATGTGAAGAGAGGAGAAGGTGGGCCGAGTCCATGGACTGGACAAGTGGGATACTTTCAGAACTACTCTCCTTGTCCGGTTTGACTCGTTTAAGTGTTTTTCTACCACCGCTTGATATTCTGCTAACAACCGATGTACTGGGCAAGAAGCTTGAGGGATTTCAGATCTCTCTAGGATGGACTAATCCATCGAGATTATATGTCCCGCCTTCTGAGAACTACTTGAGAGTTTTTGATCTTGATGCGAGCTCACTCGTGGGTAGCAATATCAGTTGGTTGCTAAACAAAACCAACACTCTTGAGTTAAGCATGAAGAATTTGACAGATCCTCTGAATGTCTTCGATTTTGCAAACTTGAATTCTCTGGAACTCGAGAACTGTGATGCTCTGGAATATCTGATTGACATGACACGTGTAAATCCTGCTACTCCAAGCAGTAGCATCTTTCCCGTCTTGAAGTCACTGCGAGTCTTTTATGCAGCAAGGCTCAAGCATATTTGTCATGGTGAGCTGCTCCACACCAAGGTATGTTTTTGTCCTCATTTTTTTTTTTTTTTGTTTGAAAGGTTGTCCTTTTCTTATTTGATCATAATCTTCTTCAGTTGTCAGACCTTTAGTTTATCTTTACTCTGCTTGTTGGTTCATTTTCCAGTCTTAACAAAACTAGAGATATGGGGCCTGGGCAACCTAACAGAGATATGGGACAGCCAACTTTCACCTGAATCCTTCTGTGAACTTAAATTTGTAGATGTGCGCGAATGTGACGAACTCTTGTGTGTGGTACCATCATATATGCAAAACAGATTGCAGAAATTGGAGCAGATAGGGATAGGTGGATGTACTTCACTAGTAGAGATTTTTGAATTCAGAAGATTTAGTGTCAATGAAGGGGATGCTTGTGAGTCAGCTGGGAAAACACTTTCACATATTTCCCAACCAGATCAAGGAACAACGCAAACAAACGAGAAGATCATGAATGTCAGACAATCTCATCAAGCCTTTCAGAATCTGACACTCCTAAAAATTTGGGATTGTCAAAGTTTGAGAACTCTGCTCTCCCTTTCAATCGCCAGAGGTCTTGTGAAGCTCCAAAGACTAGAGATAGAAAACTGCGAGGAGATTGAAGAGATAGTAGCAGCAGCAGCAGAGGGTGATGAAGAAACAGAGGATGACAACATCTTCCCTCAGCTAAGATATTTGAGTCTTGACAATCTTCCAAATCTCAGAAGCTTTTCATCATTTCGGAAATGTAGTTTGAGAACTCTGCTCTCTCCTTCAATCGCCAGAGGTCTTGTGAAGCTCCAAAGACTACAGATAGAAAACTGCGAGAAGATTGAAGAGATAGTAGTAGCAGCAGAGGGTGATGAGGAAACAGAGGATGACAACATCTTCCCTCAGCTAACATATTTGCGGCTTGAGGATCTACCCAATCTCAGAAGCTTTTCGCAAGGCAAATATAATTTCAACTGGACACTAGTGAAAAAAATAACAATCCAAGGATGCGGCAATATGAAGAAGTTTTGTTTCGGATCCGTCAGCATACCAAGGAAAGTGACGTTGGATGTTGGTGAGAATGTTTTGCAGGAGCTGAATAACAGTAGAAAAGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1268

Amino Acids

143.79

Weight (kDa)

5.47

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 179 - 345 2e-17 NB-ARC domain
LRR_14 PF23598 584 - 694 3.2e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 960 - 1103 5.1e-25 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1056 - 1140 1.7e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1147 - 1237 6.5e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 502
Acc36I ACCTGC 1 cut(s) 502
Acc65I GGTACC 1 cut(s) 2974
AccB1I GGYRCC 2 cut(s) 452, 2974
AccB7I CCANNNNNTGG 1 cut(s) 2232
AccI GTMKAC 2 cut(s) 198, 677
AccII CGCG 2 cut(s) 1056, 2949
AccIII TCCGGA 1 cut(s) 1991
AciI CCGC 7 cut(s) 597, 606, 1285, 2303, 2399, 3604, 3696
AclI AACGTT 1 cut(s) 1203
AclWI GGATC 8 cut(s) 835, 992, 1005, 2081, 2513, 3621, 3717, 3730
AcsI RAATTY 9 cut(s) 283, 401, 973, 1909, 2509, 2551, 2933, 3056, 3216
AcuI CTGAAG 5 cut(s) 214, 522, 1202, 1509, 2776
AcvI CACGTG 1 cut(s) 2606
AdeI CACNNNGTG 1 cut(s) 1973
AfaI GTAC 4 cut(s) 24, 2331, 2976, 3034
AfiI CCNNNNNNNGG 6 cut(s) 397, 986, 1884, 2232, 2716, 3137
AflII CTTAAG 1 cut(s) 770
AflIII ACRYGT 4 cut(s) 1222, 2010, 2603, 2605
AhlI ACTAGT 3 cut(s) 1753, 3040, 3667
AjiI CACGTC 2 cut(s) 133, 721
AjnI CCWGG 6 cut(s) 126, 362, 1007, 1207, 1969, 2871
AjuI GAANNNNNNNTTGG 2 cut(s) 3734, 3766
Alw21I GWGCWC 3 cut(s) 154, 420, 2444
Alw26I GTCTC 7 cut(s) 43, 139, 215, 1331, 1536, 1648, 1746
AlwI GGATC 8 cut(s) 835, 992, 1005, 2081, 2513, 3621, 3717, 3730
AlwNI CAGNNNCTG 4 cut(s) 94, 1556, 3202, 3781
Ama87I CYCGRG 1 cut(s) 2564
Aor13HI TCCGGA 1 cut(s) 1991
AoxI GGCC 3 cut(s) 1574, 2217, 2869
ApoI RAATTY 9 cut(s) 283, 401, 973, 1909, 2509, 2551, 2933, 3056, 3216
ArsI GACNNNNNNTTYG 2 cut(s) 1780, 1812
AseI ATTAAT 1 cut(s) 516
Asp700I GAANNNNTTC 6 cut(s) 90, 575, 1203, 2531, 3424, 3634
Asp718I GGTACC 1 cut(s) 2974
AspLEI GCGC 2 cut(s) 376, 2949
AspS9I GGNCC 5 cut(s) 791, 1667, 2105, 2217, 2869
AsuC2I CCSGG 1 cut(s) 171
AsuHPI GGTGA 9 cut(s) 203, 937, 1154, 1549, 2711, 2901, 3356, 3566, 3773
AsuII TTCGAA 1 cut(s) 1941
AvaI CYCGRG 1 cut(s) 2564
AvaII GGWCC 3 cut(s) 791, 1667, 2105
AxyI CCTNAGG 1 cut(s) 1578
BamHI GGATCC 2 cut(s) 997, 3722
BanI GGYRCC 2 cut(s) 452, 2974
BanII GRGCYC 3 cut(s) 154, 1323, 2444
BauI CACGAG 1 cut(s) 2447
BbrPI CACGTG 1 cut(s) 2606
BbsI GAAGAC 3 cut(s) 623, 1031, 2524
Bbv12I GWGCWC 3 cut(s) 154, 420, 2444
BbvCI CCTCAGC 3 cut(s) 2091, 3378, 3588
BccI CCATC 7 cut(s) 547, 622, 1561, 1768, 2364, 2389, 2986
BceAI ACGGC 2 cut(s) 1146, 1457
BciT130I CCWGG 6 cut(s) 128, 364, 1009, 1209, 1971, 2873
BciVI GTATCC 1 cut(s) 2238
BclI TGATCA 1 cut(s) 2778
BcnI CCSGG 1 cut(s) 171
BcoDI GTCTC 7 cut(s) 43, 139, 215, 1331, 1536, 1648, 1746
BcuI ACTAGT 3 cut(s) 1753, 3040, 3667
BfmI CTRYAG 4 cut(s) 155, 243, 465, 3503
BfrI CTTAAG 1 cut(s) 770
BfuAI ACCTGC 1 cut(s) 502
BfuI GTATCC 1 cut(s) 2238
BglII AGATCT 3 cut(s) 943, 1711, 2358
Bme1390I CCNGG 7 cut(s) 128, 171, 364, 1009, 1209, 1971, 2873
Bme18I GGWCC 3 cut(s) 791, 1667, 2105
BmeT110I CYCGRG 1 cut(s) 2564
BmgBI CACGTC 2 cut(s) 133, 721
BmgT120I GGNCC 5 cut(s) 791, 1667, 2105, 2217, 2869
BmiI GGNNCC 7 cut(s) 436, 454, 999, 2107, 2870, 2976, 3724
BmrFI CCNGG 7 cut(s) 128, 171, 364, 1009, 1209, 1971, 2873
BmrI ACTGGG 1 cut(s) 2342
BmuI ACTGGG 1 cut(s) 2342
BpiI GAAGAC 3 cut(s) 623, 1031, 2524
BplI GAGNNNNNCTC 4 cut(s) 136, 168, 1726, 1758
BpmI CTGGAG 1 cut(s) 1030
Bpu10I CCTNAGC 4 cut(s) 1844, 2091, 3378, 3588
Bpu14I TTCGAA 1 cut(s) 1941
BpuEI CTTGAG 8 cut(s) 548, 1313, 1700, 2366, 2437, 2513, 2666, 3629
BpuMI CCSGG 1 cut(s) 171
BsaAI YACGTR 2 cut(s) 812, 2606
BsaBI GATNNNNATC 1 cut(s) 2783
BsaI GGTCTC 2 cut(s) 43, 1331
BsaJI CCNNGG 8 cut(s) 362, 363, 826, 2226, 2715, 2872, 3688, 3737
BsaWI WCCGGW 2 cut(s) 1991, 2270
Bsc4I CCNNNNNNNGG 6 cut(s) 397, 986, 1884, 2232, 2716, 3137
Bse1I ACTGG 4 cut(s) 2237, 2337, 2843, 3665
Bse21I CCTNAGG 1 cut(s) 1578
Bse3DI GCAATG 2 cut(s) 1107, 1149
Bse8I GATNNNNATC 1 cut(s) 2783
BseAI TCCGGA 1 cut(s) 1991
BseBI CCWGG 6 cut(s) 128, 364, 1009, 1209, 1971, 2873
BseDI CCNNGG 8 cut(s) 362, 363, 826, 2226, 2715, 2872, 3688, 3737
BseJI GATNNNNATC 1 cut(s) 2783
BseLI CCNNNNNNNGG 6 cut(s) 397, 986, 1884, 2232, 2716, 3137
BseMI GCAATG 2 cut(s) 1107, 1149
BseNI ACTGG 4 cut(s) 2237, 2337, 2843, 3665
BseRI GAGGAG 7 cut(s) 129, 132, 233, 451, 2186, 2221, 3323
BseYI CCCAGC 2 cut(s) 1097, 3101
Bsh1236I CGCG 2 cut(s) 1056, 2949
BshFI GGCC 3 cut(s) 1576, 2219, 2871
BshNI GGYRCC 2 cut(s) 452, 2974
BsiHKAI GWGCWC 3 cut(s) 154, 420, 2444
BsiHKCI CYCGRG 1 cut(s) 2564
BsiSI CCGG 4 cut(s) 171, 995, 1992, 2271
BslFI GGGAC 5 cut(s) 153, 905, 1742, 2381, 2909
BslI CCNNNNNNNGG 6 cut(s) 397, 986, 1884, 2232, 2716, 3137
BsmAI GTCTC 7 cut(s) 43, 139, 215, 1331, 1536, 1648, 1746
BsmBI CGTCTC 2 cut(s) 139, 215
BsmFI GGGAC 5 cut(s) 153, 905, 1742, 2381, 2909
BsmI GAATGC 2 cut(s) 1171, 1452
BsnI GGCC 3 cut(s) 1576, 2219, 2871
Bso31I GGTCTC 2 cut(s) 43, 1331
BsoBI CYCGRG 1 cut(s) 2564
Bsp119I TTCGAA 1 cut(s) 1941
Bsp1286I GDGCHC 4 cut(s) 154, 420, 1323, 2444
Bsp13I TCCGGA 1 cut(s) 1991
Bsp19I CCATGG 1 cut(s) 2226
Bsp68I TCGCGA 1 cut(s) 1056
BspACI CCGC 7 cut(s) 597, 606, 1285, 2303, 2399, 3604, 3696
BspANI GGCC 3 cut(s) 1576, 2219, 2871
BspEI TCCGGA 1 cut(s) 1991
BspFNI CGCG 2 cut(s) 1056, 2949
BspHI TCATGA 2 cut(s) 1854, 3165
BspLI GGNNCC 7 cut(s) 436, 454, 999, 2107, 2870, 2976, 3724
BspMAI CTGCAG 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 502
BspPI GGATC 8 cut(s) 835, 992, 1005, 2081, 2513, 3621, 3717, 3730
BspT104I TTCGAA 1 cut(s) 1941
BspT107I GGYRCC 2 cut(s) 452, 2974
BspTI CTTAAG 1 cut(s) 770
BspTNI GGTCTC 2 cut(s) 43, 1331
BsrDI GCAATG 2 cut(s) 1107, 1149
BsrI ACTGG 4 cut(s) 2237, 2337, 2843, 3665
BssECI CCNNGG 8 cut(s) 362, 363, 826, 2226, 2715, 2872, 3688, 3737
BssNAI GTATAC 1 cut(s) 678
BssSI CACGAG 1 cut(s) 2447
BssT1I CCWWGG 5 cut(s) 826, 2226, 2715, 3688, 3737
Bst1107I GTATAC 1 cut(s) 678
Bst2BI CACGAG 1 cut(s) 2447
Bst2UI CCWGG 6 cut(s) 128, 364, 1009, 1209, 1971, 2873
Bst4CI ACNGT 8 cut(s) 66, 244, 1123, 1373, 1558, 1771, 2573, 3791
Bst6I CTCTTC 6 cut(s) 210, 1485, 2123, 2196, 3312, 3525
BstAFI CTTAAG 1 cut(s) 770
BstBAI YACGTR 2 cut(s) 812, 2606
BstBI TTCGAA 1 cut(s) 1941
BstC8I GCNNGC 6 cut(s) 185, 262, 457, 1268, 1272, 2440
BstDSI CCRYGG 1 cut(s) 2226
BstFNI CGCG 2 cut(s) 1056, 2949
BstHHI GCGC 2 cut(s) 376, 2949
BstMAI GTCTC 7 cut(s) 43, 139, 215, 1331, 1536, 1648, 1746
BstMWI GCNNNNNNNGC 8 cut(s) 41, 149, 254, 257, 380, 603, 606, 3335
BstNI CCWGG 6 cut(s) 128, 364, 1009, 1209, 1971, 2873
BstNSI RCATGY 2 cut(s) 1226, 2014
BstSCI CCNGG 7 cut(s) 126, 169, 362, 1007, 1207, 1969, 2871
BstSFI CTRYAG 4 cut(s) 155, 243, 465, 3503
BstUI CGCG 2 cut(s) 1056, 2949
BstV2I GAAGAC 3 cut(s) 623, 1031, 2524
BstX2I RGATCY 9 cut(s) 840, 943, 997, 1711, 2073, 2358, 2518, 3613, 3722
BstYI RGATCY 9 cut(s) 840, 943, 997, 1711, 2073, 2358, 2518, 3613, 3722
BstZ17I GTATAC 1 cut(s) 678
Bsu36I CCTNAGG 1 cut(s) 1578
BsuI GTATCC 1 cut(s) 2238
BsuRI GGCC 3 cut(s) 1576, 2219, 2871
BtgI CCRYGG 1 cut(s) 2226
BtgZI GCGATG 1 cut(s) 1440
BtrI CACGTC 2 cut(s) 133, 721
BtsI GCAGTG 2 cut(s) 51, 2654
BtsIMutI CAGTG 4 cut(s) 51, 238, 1616, 2654
BtuMI TCGCGA 1 cut(s) 1056
BveI ACCTGC 1 cut(s) 502
Cac8I GCNNGC 6 cut(s) 185, 262, 457, 1268, 1272, 2440
CaiI CAGNNNCTG 4 cut(s) 94, 1556, 3202, 3781
CciI TCATGA 2 cut(s) 1854, 3165
CfoI GCGC 2 cut(s) 376, 2949
Cfr13I GGNCC 5 cut(s) 791, 1667, 2105, 2217, 2869
CsiI ACCWGGT 1 cut(s) 1969
Csp6I GTAC 4 cut(s) 23, 2330, 2975, 3033
CviQI GTAC 4 cut(s) 23, 2330, 2975, 3033
DraI TTTAAA 3 cut(s) 1339, 1776, 1906
DraIII CACNNNGTG 1 cut(s) 1973
Eam1104I CTCTTC 6 cut(s) 210, 1485, 2123, 2196, 3312, 3525
EarI CTCTTC 6 cut(s) 210, 1485, 2123, 2196, 3312, 3525
Ecl136II GAGCTC 2 cut(s) 152, 2442
Eco130I CCWWGG 5 cut(s) 826, 2226, 2715, 3688, 3737
Eco24I GRGCYC 3 cut(s) 154, 1323, 2444
Eco31I GGTCTC 2 cut(s) 43, 1331
Eco47I GGWCC 3 cut(s) 791, 1667, 2105
Eco53kI GAGCTC 2 cut(s) 152, 2442
Eco57I CTGAAG 5 cut(s) 214, 522, 1202, 1509, 2776
Eco72I CACGTG 1 cut(s) 2606
Eco81I CCTNAGG 1 cut(s) 1578
Eco88I CYCGRG 1 cut(s) 2564
EcoICRI GAGCTC 2 cut(s) 152, 2442
EcoO109I RGGNCCY 1 cut(s) 2869
EcoRI GAATTC 3 cut(s) 401, 2551, 3056
EcoRII CCWGG 6 cut(s) 126, 362, 1007, 1207, 1969, 2871
EcoT14I CCWWGG 5 cut(s) 826, 2226, 2715, 3688, 3737
EcoT22I ATGCAT 1 cut(s) 1452
EcoT38I GRGCYC 3 cut(s) 154, 1323, 2444
ErhI CCWWGG 5 cut(s) 826, 2226, 2715, 3688, 3737
Esp3I CGTCTC 2 cut(s) 139, 215
FalI AAGNNNNNCTT 4 cut(s) 674, 706, 726, 758
FaqI GGGAC 5 cut(s) 153, 905, 1742, 2381, 2909
FauI CCCGC 1 cut(s) 2406
FauNDI CATATG 1 cut(s) 593
FbaI TGATCA 1 cut(s) 2778
FblI GTMKAC 2 cut(s) 198, 677
FriOI GRGCYC 3 cut(s) 154, 1323, 2444
GlaI GCGC 2 cut(s) 375, 2948
GsaI CCCAGC 2 cut(s) 1101, 3105
GsuI CTGGAG 1 cut(s) 1030
HaeIII GGCC 3 cut(s) 1576, 2219, 2871
HapII CCGG 4 cut(s) 171, 995, 1992, 2271
HhaI GCGC 2 cut(s) 376, 2949
Hin6I GCGC 2 cut(s) 374, 2947
HinP1I GCGC 2 cut(s) 374, 2947
HindIII AAGCTT 3 cut(s) 2342, 3421, 3631
HpaII CCGG 4 cut(s) 171, 995, 1992, 2271
HphI GGTGA 9 cut(s) 203, 937, 1154, 1549, 2711, 2901, 3356, 3566, 3773
Hpy166II GTNNAC 8 cut(s) 124, 199, 678, 917, 1259, 1616, 2119, 2927
Hpy8I GTNNAC 8 cut(s) 124, 199, 678, 917, 1259, 1616, 2119, 2927
HpyCH4III ACNGT 8 cut(s) 66, 244, 1123, 1373, 1558, 1771, 2573, 3791
HpyCH4IV ACGT 6 cut(s) 132, 720, 811, 1203, 2605, 3749
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 149, 254, 257, 380, 603, 606, 3335
HpySE526I ACGT 6 cut(s) 132, 720, 811, 1203, 2605, 3749
HspAI GCGC 2 cut(s) 374, 2947
Kpn2I TCCGGA 1 cut(s) 1991
KpnI GGTACC 1 cut(s) 2978
Ksp22I TGATCA 1 cut(s) 2778
LmnI GCTCC 8 cut(s) 391, 464, 718, 2712, 3010, 3285, 3498, 3778
MabI ACCWGGT 1 cut(s) 1969
MaeII ACGT 6 cut(s) 132, 720, 811, 1203, 2605, 3749
MaeIII GTNAC 6 cut(s) 104, 133, 925, 2652, 2954, 3745
MflI RGATCY 9 cut(s) 840, 943, 997, 1711, 2073, 2358, 2518, 3613, 3722
MhlI GDGCHC 4 cut(s) 154, 420, 1323, 2444
MlyI GAGTC 6 cut(s) 1021, 2231, 2272, 2670, 3104, 3403
MmeI TCCRAC 5 cut(s) 430, 1105, 1645, 2083, 3732
Mph1103I ATGCAT 1 cut(s) 1452
MroI TCCGGA 1 cut(s) 1991
MroXI GAANNNNTTC 6 cut(s) 90, 575, 1203, 2531, 3424, 3634
MslI CAYNNNNRTG 2 cut(s) 450, 1227
MspA1I CMGCKG 2 cut(s) 2150, 3101
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 4 cut(s) 171, 995, 1992, 2271
MspR9I CCNGG 7 cut(s) 128, 171, 364, 1009, 1209, 1971, 2873
Mva1269I GAATGC 2 cut(s) 1171, 1452
MvaI CCWGG 6 cut(s) 128, 364, 1009, 1209, 1971, 2873
MvnI CGCG 2 cut(s) 1056, 2949
MwoI GCNNNNNNNGC 8 cut(s) 41, 149, 254, 257, 380, 603, 606, 3335
NciI CCSGG 1 cut(s) 171
NcoI CCATGG 1 cut(s) 2226
NdeI CATATG 1 cut(s) 593
NlaIV GGNNCC 7 cut(s) 436, 454, 999, 2107, 2870, 2976, 3724
NmeAIII GCCGAG 1 cut(s) 2245
NmuCI GTSAC 6 cut(s) 104, 133, 925, 2652, 2954, 3745
NruI TCGCGA 1 cut(s) 1056
NsiI ATGCAT 1 cut(s) 1452
NspI RCATGY 2 cut(s) 1226, 2014
NspV TTCGAA 1 cut(s) 1941
PaeR7I CTCGAG 1 cut(s) 2564
PagI TCATGA 2 cut(s) 1854, 3165
PaqCI CACCTGC 1 cut(s) 502
PasI CCCWGGG 1 cut(s) 363
PciI ACATGT 2 cut(s) 1222, 2010
PcsI WCGNNNNNNNCGW 1 cut(s) 2064
PctI GAATGC 2 cut(s) 1171, 1452
PdmI GAANNNNTTC 6 cut(s) 90, 575, 1203, 2531, 3424, 3634
PflMI CCANNNNNTGG 1 cut(s) 2232
PfoI TCCNGGA 2 cut(s) 169, 1207
PleI GAGTC 6 cut(s) 1020, 2230, 2272, 2669, 3103, 3402
PmaCI CACGTG 1 cut(s) 2606
PmlI CACGTG 1 cut(s) 2606
PpsI GAGTC 6 cut(s) 1020, 2230, 2272, 2669, 3103, 3402
Ppu21I YACGTR 2 cut(s) 812, 2606
PscI ACATGT 2 cut(s) 1222, 2010
PshBI ATTAAT 1 cut(s) 516
Psp124BI GAGCTC 2 cut(s) 154, 2444
Psp1406I AACGTT 1 cut(s) 1203
Psp6I CCWGG 6 cut(s) 126, 362, 1007, 1207, 1969, 2871
PspCI CACGTG 1 cut(s) 2606
PspFI CCCAGC 2 cut(s) 1097, 3101
PspGI CCWGG 6 cut(s) 126, 362, 1007, 1207, 1969, 2871
PspN4I GGNNCC 7 cut(s) 436, 454, 999, 2107, 2870, 2976, 3724
PspPI GGNCC 5 cut(s) 791, 1667, 2105, 2217, 2869
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 4 cut(s) 94, 1556, 3202, 3781
PsuI RGATCY 9 cut(s) 840, 943, 997, 1711, 2073, 2358, 2518, 3613, 3722
PvuII CAGCTG 2 cut(s) 2150, 3101
RruI TCGCGA 1 cut(s) 1056
RsaI GTAC 4 cut(s) 24, 2331, 2976, 3034
RsaNI GTAC 4 cut(s) 23, 2330, 2975, 3033
RseI CAYNNNNRTG 2 cut(s) 450, 1227
SacI GAGCTC 2 cut(s) 154, 2444
Sau96I GGNCC 5 cut(s) 791, 1667, 2105, 2217, 2869
SchI GAGTC 6 cut(s) 1021, 2231, 2272, 2670, 3104, 3403
ScrFI CCNGG 7 cut(s) 128, 171, 364, 1009, 1209, 1971, 2873
SduI GDGCHC 4 cut(s) 154, 420, 1323, 2444
SexAI ACCWGGT 1 cut(s) 1969
SfcI CTRYAG 4 cut(s) 155, 243, 465, 3503
Sfr274I CTCGAG 1 cut(s) 2564
SfuI TTCGAA 1 cut(s) 1941
SinI GGWCC 3 cut(s) 791, 1667, 2105
SlaI CTCGAG 1 cut(s) 2564
SmiMI CAYNNNNRTG 2 cut(s) 450, 1227
SpeI ACTAGT 3 cut(s) 1753, 3040, 3667
SsiI CCGC 7 cut(s) 597, 606, 1285, 2303, 2399, 3604, 3696
SspI AATATT 1 cut(s) 514
SstI GAGCTC 2 cut(s) 154, 2444
StyD4I CCNGG 7 cut(s) 126, 169, 362, 1007, 1207, 1969, 2871
StyI CCWWGG 5 cut(s) 826, 2226, 2715, 3688, 3737
TaaI ACNGT 8 cut(s) 66, 244, 1123, 1373, 1558, 1771, 2573, 3791
TaiI ACGT 6 cut(s) 135, 723, 814, 1206, 2608, 3752
TaqI TCGA 7 cut(s) 669, 1044, 1941, 2058, 2384, 2535, 2565
TaqII GACCGA 1 cut(s) 1313
TatI WGTACW 2 cut(s) 2329, 3032
TauI GCSGC 5 cut(s) 600, 609, 1287, 3607, 3699
TscAI CASTG 4 cut(s) 51, 238, 1623, 2661
TseFI GTSAC 6 cut(s) 104, 133, 925, 2652, 2954, 3745
Tsp45I GTSAC 6 cut(s) 104, 133, 925, 2652, 2954, 3745
TspGWI ACGGA 1 cut(s) 3715
TspRI CASTG 4 cut(s) 51, 238, 1623, 2661
Van91I CCANNNNNTGG 1 cut(s) 2232
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 3 cut(s) 791, 1667, 2105
VspI ATTAAT 1 cut(s) 516
XapI RAATTY 9 cut(s) 283, 401, 973, 1909, 2509, 2551, 2933, 3056, 3216
XbaI TCTAGA 1 cut(s) 1798
XceI RCATGY 2 cut(s) 1226, 2014
XcmI CCANNNNNNNNNTGG 2 cut(s) 36, 885
XhoI CTCGAG 1 cut(s) 2564
XmiI GTMKAC 2 cut(s) 198, 677
XmnI GAANNNNTTC 6 cut(s) 90, 575, 1203, 2531, 3424, 3634
Zsp2I ATGCAT 1 cut(s) 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.