Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0000203

Physical Location & Seq

Genomic Coordinates Reverse (-)

34710316 .. 34715547

Gene Structure 5232 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00312030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3456 bp

ATGCAAAATATTATTCTAGCAGTTGCTACACGATTGGCAGAATGGACGGTGTTTATTTGGCGGCAGTTCAGTTATGTGGTTCAGTACAAAAGCAACATTGAGCATCTCAACAGACAGGTTCAGGAACTGCGTGATAAAAGATATGGAGTGGACATGGAGGTGAAACCTGCTACAGAAGCTTCGAAGATTATTGATCCTGGAGTTCAACATTGGCTGGCTGAGGTAGACAAGCTGATAGGAGAAAAGGAGAAGTGTTTCAATGAAGAGACTGTAGCAACAAAGGCAGCATGCTGTAATGGGTCTTTTCCAGATTTGAAGTGCCGTTATTCTTTAGGAAGGAAAGCTAAGAAGATGACTATGGTTTTTGATTACCTCCTTGCTAAAGATCTTGGGACTGCAGCGCATCCTGCTCTTCCCCCAGAGGTGGAATTTCAACCCACACAATATTCTGAGGATTCTTCTCAGCTTGCAACCAACTTCCAGGAAGGTGCCAGCTCCTCAGAAACATCTAACCAAATTCAGCAGCCTACATCTACAGAAAAAAATATTGATTTTGAATCGAGGAGATCGACCATTAGAAGTGTAATGGAAGCTGTGAAGGGGAATGAGATCAATCCGGTTGTCATATGCGGCATGGGAGGTATTGGGAAGACAACATTGATGGGGGAAGTTGTTAACAGAGCAAAGAACGAGGGTCTGTTTGATGAGTATACAAAGGCAACTGTTAAAGAAACTCTTGACAAAACTCCTGACATTTGTCTGATTCAAGATGAACTTGCTCGATATCTAGGTCTTCCACTTGAAGGAAAAGAAATAGATGCAAGAGCAAACAAGATACGTCAAAGATTATCTGCAGGTGCCAAGAAGGTTCTTGTAATATTAGACAATGTTTCGACAACAAACCCAGACTTTTTGTGGGAAGTAGGGATTCCTCGTGATCAAAATAGTTGTAAGCTTTTAGTAACATCAAGAGAACGAGATGTATTCAAGGACATTGACACAAAAAAGAATTTCCCCATCAGTGATTTGCCAGAAAATGAGGCCTGGAGCTTGTTCAAGAAGACAGCAGGAGGCTTGATTGAATCTGATCCCGATTTGCATTTGGTAGCAAAACAGGTTGTGAAGGAATGTGCAGGGTTGCCAATTGCAATTTCAACTGTTGGAAGGGCACTACATGGTGAAGGAATTGCAATTTGGAAGAATGCACTTAGGGAAATGGAAAAGGCTTGCCCAGAAAATGTTCCAGGAGTGATAGGATCATCCTCTGAAGGGTTGACTCAAAAGAAAAGTGATGAGGTTAAAGAGAGAATTGTGGTAAGACACTCGGTGGAAACTGTGAAGTCTATGGGTTGGCCAAAGAGTAGTGTTTCCGAGCAATCTACTTACAGCAGTTCACTGTGTATTGGTGGGCTTTCGGAGCTCTTACTGTTAAGATGCGTAACTGAAAGTGGATTGGAGCCGCCACCCACAATTTTCAATAGAATGGAAGATCTCAAGGTTCTCGTAATTGCAGGTATTTCTCTATTGTCAATACTGCCATCACTTCCAGTTTTGAGAAACCTTCAAACAACATTGTGTTTGGAGGATTGCTATCTAAACTTTGATGTAGTATCTGTCATTGGGGAGATAAGGACACTCATGATACTCAGTTTACGTGGATCTAACATCAAACGACTGCCTGATACATTTAAAAATTTATCTAATCTAAGATTACTTGATTTGTCGAGATGTATGAAACTTGAGATAATCTCACCAGGTGTCACATCAAGTTTATCCCGACTAGAAGAGTTGCATATGTGGGATAGCTTTGAACATTGGGTGGTTGAGAAACTAGCCTCAACAGAAACAACCAATTGGATTTCTCAATTGGAAGCTGAGACAAGACCTAAACCAGAAGAACTCCACGTGGAACTGGAGATGTGGAAGAGATTCAACAGATTGGCGGTTGAGCAACCAGCATATCTAGGAAAGATGTACAGAGAGAAGGTTCAAAATTTATATACGAAAGACGAGGAGCACACATACAGAAGGTGGGCCAACTCCATGGACTGGAAAATTGGGATACTTTCAGAGCTACTTTCCTTGTCCAATTTGACTGCTTTAGAAGTTGTTCTACCACCGGTTGATAGTCTGGTAACCAGCAAGCTATTTGACAAGCTTGAAAGATTTAAGATCTCTATAGGATGGAAGGAGCATATATATAATTGGGAATTAGAGCCTTATGGAAACTACTTGAGAGTTCATGATCTTGATGCAAGCTCTCTTTCTCTTGCACTCGAGGAGTGTGAATCTTTGGAATATCTGATTGACATGACACTTGTAAAACCTACACCACATAGTAGTGTCTTTCCTGTCCTGGATCGTCTGGTTGTATTTGGAGGAAGTAGGCTGAAGGAGATTTTTCATGGTGAGCTGCCAATGGGATCTCTTAGGAAACTACGTGAGATGGATTTACTATTCCTAAAAATAAAAAACTGCCCGTCGTTGGAAGAGATCGTTGCCTTGGAAGGATCAAGACATCAGGAGGAAGGAGCAACCAAAGAGATCAACTTCCAAGGATTGACAGAGTTGACTCTTGCAGATCTACCTAGTTTCACTGGGATAAGCAAAAATATCTATAAAAAGGAGTTGGAAAGGCTACCGGATGTTATCAGTCTCTATCCAAATGTTTTGACAGTGGAACATTCTAATGTGTCCATACAACATCTGTTTGACACCAAGGTTCAATTTCCGGTTTTACGAAGACTACAAATTGAAGGCATGGACAACTTAAAAGGGATATGGAACAGCGAACTTTCACCTGATTCCTTCTGTGAACTAAAATTTCTAATAGTGAACAGTTGTAACAAACTATTGCATTTGGTGCCAACACATATGCAAAATAGATTGCAGAAAATGGAGTTCATATGTGCCTCACACTGTTCTTCACTAGAAGAGATCTTTGATTTCAAGAGATCACCTTCACATATTTCCCAACCTGGCCAAGGGATGCAAAGGAGCAAGATCATGGATTTCAAACAATCTTGTCAAGCCTTTCCGAATCTAACAGATATATCACTCTGGGAATGTAACAGTGTTAGAATTGTGCTCTCTCCTTCAATTTCCGGGGGTCTTGTGAAACTCAAAGTACTAAGGATACGGCGGTGCAAGAAGATTGAAGAAATAGTAGCAGCAGCAGCAGAGGGTGAAGAAACAGAGGATAACAACATGTTTCCTCAGCTGAGTAAATTGGTACTTGAGGATCTACCAAATCTTCGAAGCTTTTCAGAAGGCAAATATAATTTTGAATGGCCACTGCTGAAATGTATAGATATTGGAAAATGCTATATGAAGCATTTTTGTTTTGGATCCCTCAGCATTCCAAGATTTGGGCACTATGCTTCATTTGCTCGTGGGGGAGTGAGTATGCAGGAGCTGCTTGGTACAGCTTGGGTTACGGTTATGCTTCAGGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_8 NB-ARC

Protein Analysis

1151

Amino Acids

130.14

Weight (kDa)

5.89

Isoelectric Point (pI)

44.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATPase_2	PF01637	187 - 300	1.6e-06	ATPase domain predominantly from Archaea
NB-ARC	PF00931	192 - 356	8.9e-21	NB-ARC domain
LRR_8	PF13855	544 - 599	1.5e-07	Leucine rich repeat
LRR_RPS2	PF23247	915 - 987	2.1e-13	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1007 - 1108	1.9e-09	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000203)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica	Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis	pycom05g14750
rosa_chinensis	RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata	RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora	Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii	Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa	Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha	Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana	Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	845
Acc36I	ACCTGC	3 cut(s)	175, 845, 1502
AccB1I	GGYRCC	3 cut(s)	488, 857, 2863
AccB7I	CCANNNNNTGG	2 cut(s)	2049, 3368
AccI	GTMKAC	2 cut(s)	225, 710
AciI	CCGC	5 cut(s)	61, 630, 1460, 1943, 3142
AcoI	YGGCCR	3 cut(s)	1352, 2980, 3290
AcsI	RAATTY	6 cut(s)	428, 516, 1009, 1693, 1993, 2822
AcuI	CTGAAG	3 cut(s)	1287, 2411, 3431
AcvI	CACGTG	1 cut(s)	1906
AdeI	CACNNNGTG	2 cut(s)	1327, 1757
AfaI	GTAC	5 cut(s)	86, 1976, 3129, 3234, 3424
AfiI	CCNNNNNNNGG	7 cut(s)	424, 803, 1269, 2049, 2485, 2984, 3368
AflIII	ACRYGT	1 cut(s)	3207
AgeI	ACCGGT	1 cut(s)	2119
AjnI	CCWGG	7 cut(s)	196, 480, 1043, 1243, 1753, 2355, 2977
AjuI	GAANNNNNNNTTGG	4 cut(s)	1135, 1167, 1177, 1209
Alw21I	GWGCWC	3 cut(s)	1422, 2019, 3090
Alw26I	GTCTC	3 cut(s)	260, 1871, 2660
AlwNI	CAGNNNCTG	2 cut(s)	127, 3415
Ama87I	CYCGRG	1 cut(s)	2276
AoxI	GGCC	5 cut(s)	1041, 1352, 2034, 2980, 3290
ApeKI	GCWGC	8 cut(s)	284, 398, 523, 2413, 3170, 3173, 3176, 3415
ApoI	RAATTY	6 cut(s)	428, 516, 1009, 1693, 1993, 2822
AsiGI	ACCGGT	1 cut(s)	2119
Asp700I	GAANNNNTTC	5 cut(s)	254, 1239, 1928, 2109, 3262
AspLEI	GCGC	1 cut(s)	403
AspS9I	GGNCC	1 cut(s)	2034
AsuC2I	CCSGG	1 cut(s)	3106
AsuHPI	GGTGA	7 cut(s)	172, 1190, 1743, 2420, 2790, 2949, 3197
AsuII	TTCGAA	2 cut(s)	182, 3256
AvaI	CYCGRG	1 cut(s)	2276
BaeGI	GKGCMC	2 cut(s)	1171, 3375
BaeI	ACNNNNGTAYC	2 cut(s)	3128, 3161
BalI	TGGCCA	3 cut(s)	1354, 2982, 3292
BamHI	GGATCC	1 cut(s)	3347
BanI	GGYRCC	3 cut(s)	488, 857, 2863
BanII	GRGCYC	1 cut(s)	1422
BarI	GAAGNNNNNNTAC	2 cut(s)	2097, 2129
BauI	CACGAG	2 cut(s)	933, 3390
BbrPI	CACGTG	1 cut(s)	1906
BbsI	GAAGAC	4 cut(s)	656, 785, 1067, 2749
Bbv12I	GWGCWC	3 cut(s)	1422, 2019, 3090
BbvCI	CCTCAGC	3 cut(s)	219, 3216, 3353
BbvI	GCAGC	8 cut(s)	296, 410, 535, 2400, 3182, 3185, 3188, 3402
BccI	CCATC	5 cut(s)	655, 1025, 1546, 2178, 2440
BceAI	ACGGC	2 cut(s)	306, 3155
BciT130I	CCWGG	7 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979
BciVI	GTATCC	2 cut(s)	2055, 3129
BclI	TGATCA	1 cut(s)	937
BcnI	CCSGG	1 cut(s)	3106
BcoDI	GTCTC	3 cut(s)	260, 1871, 2660
BfaI	CTAG	7 cut(s)	17, 788, 1781, 1832, 1964, 2589, 2930
BfmI	CTRYAG	6 cut(s)	171, 270, 396, 534, 852, 2178
BfuAI	ACCTGC	3 cut(s)	175, 845, 1502
BfuI	GTATCC	2 cut(s)	2055, 3129
BglII	AGATCT	5 cut(s)	385, 1489, 2172, 2581, 2937
BmcAI	AGTACT	1 cut(s)	3129
Bme1390I	CCNGG	8 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979, 3106
BmeT110I	CYCGRG	1 cut(s)	2276
BmgT120I	GGNCC	1 cut(s)	2034
BmiI	GGNNCC	5 cut(s)	490, 859, 1458, 2865, 3349
BmrFI	CCNGG	8 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979, 3106
BmrI	ACTGGG	1 cut(s)	2607
BmsI	GCATC	6 cut(s)	112, 412, 808, 1424, 2242, 2979
BmuI	ACTGGG	1 cut(s)	2607
BoxI	GACNNNNGTC	1 cut(s)	756
BpiI	GAAGAC	4 cut(s)	656, 785, 1067, 2749
BplI	GAGNNNNNCTC	2 cut(s)	1733, 1765
BpmI	CTGGAG	3 cut(s)	219, 1066, 1934
Bpu10I	CCTNAGC	3 cut(s)	219, 3216, 3353
Bpu14I	TTCGAA	2 cut(s)	182, 3256
BpuEI	CTTGAG	4 cut(s)	1478, 1760, 2254, 3257
BpuMI	CCSGG	1 cut(s)	3106
BsaAI	YACGTR	3 cut(s)	1655, 1906, 2441
BsaBI	GATNNNNATC	1 cut(s)	1590
BsaJI	CCNNGG	6 cut(s)	2043, 2502, 2554, 2718, 2983, 3105
BsaWI	WCCGGW	4 cut(s)	616, 2119, 2641, 2731
Bsc4I	CCNNNNNNNGG	7 cut(s)	424, 803, 1269, 2049, 2485, 2984, 3368
Bse118I	RCCGGY	1 cut(s)	2119
Bse1I	ACTGG	4 cut(s)	1547, 1917, 2054, 2602
Bse8I	GATNNNNATC	1 cut(s)	1590
BseBI	CCWGG	7 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979
BseDI	CCNNGG	6 cut(s)	2043, 2502, 2554, 2718, 2983, 3105
BseGI	GGATG	5 cut(s)	403, 1259, 2189, 2650, 2994
BseJI	GATNNNNATC	1 cut(s)	1590
BseLI	CCNNNNNNNGG	7 cut(s)	424, 803, 1269, 2049, 2485, 2984, 3368
BseMII	CTCAG	9 cut(s)	210, 441, 476, 513, 1660, 1866, 3212, 3230, 3367
BseNI	ACTGG	4 cut(s)	1547, 1917, 2054, 2602
BseRI	GAGGAG	4 cut(s)	487, 577, 2027, 2294
BseSI	GKGCMC	2 cut(s)	1171, 3375
BseXI	GCAGC	8 cut(s)	296, 410, 535, 2400, 3182, 3185, 3188, 3402
BsgI	GTGCAG	1 cut(s)	1152
BshFI	GGCC	5 cut(s)	1043, 1354, 2036, 2982, 3292
BshNI	GGYRCC	3 cut(s)	488, 857, 2863
BshTI	ACCGGT	1 cut(s)	2119
BsiHKAI	GWGCWC	3 cut(s)	1422, 2019, 3090
BsiHKCI	CYCGRG	1 cut(s)	2276
BsiSI	CCGG	5 cut(s)	617, 2120, 2642, 2732, 3105
BslFI	GGGAC	1 cut(s)	406
BslI	CCNNNNNNNGG	7 cut(s)	424, 803, 1269, 2049, 2485, 2984, 3368
BsmAI	GTCTC	3 cut(s)	260, 1871, 2660
BsmFI	GGGAC	1 cut(s)	406
BsmI	GAATGC	2 cut(s)	1207, 3357
BsnI	GGCC	5 cut(s)	1043, 1354, 2036, 2982, 3292
BsoBI	CYCGRG	1 cut(s)	2276
Bsp119I	TTCGAA	2 cut(s)	182, 3256
Bsp1286I	GDGCHC	5 cut(s)	1171, 1422, 2019, 3090, 3375
Bsp1407I	TGTACA	1 cut(s)	1974
Bsp19I	CCATGG	1 cut(s)	2043
BspACI	CCGC	5 cut(s)	61, 630, 1460, 1943, 3142
BspANI	GGCC	5 cut(s)	1043, 1354, 2036, 2982, 3292
BspCNI	CTCAG	9 cut(s)	211, 442, 475, 512, 1659, 1867, 3213, 3229, 3366
BspHI	TCATGA	2 cut(s)	1638, 2242
BspLI	GGNNCC	5 cut(s)	490, 859, 1458, 2865, 3349
BspMAI	CTGCAG	2 cut(s)	400, 856
BspMI	ACCTGC	3 cut(s)	175, 845, 1502
BspQI	GCTCTTC	1 cut(s)	417
BspT104I	TTCGAA	2 cut(s)	182, 3256
BspT107I	GGYRCC	3 cut(s)	488, 857, 2863
BsrFI	RCCGGY	1 cut(s)	2119
BsrGI	TGTACA	1 cut(s)	1974
BsrI	ACTGG	4 cut(s)	1547, 1917, 2054, 2602
BssAI	RCCGGY	1 cut(s)	2119
BssECI	CCNNGG	6 cut(s)	2043, 2502, 2554, 2718, 2983, 3105
BssNAI	GTATAC	1 cut(s)	711
BssSI	CACGAG	2 cut(s)	933, 3390
BssT1I	CCWWGG	5 cut(s)	2043, 2502, 2554, 2718, 2983
Bst1107I	GTATAC	1 cut(s)	711
Bst2BI	CACGAG	2 cut(s)	933, 3390
Bst2UI	CCWGG	7 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979
Bst6I	CTCTTC	6 cut(s)	258, 417, 1779, 1919, 2484, 2928
BstAPI	GCANNNNNTGC	2 cut(s)	2863, 3415
BstAUI	TGTACA	1 cut(s)	1974
BstBAI	YACGTR	3 cut(s)	1655, 1906, 2441
BstBI	TTCGAA	2 cut(s)	182, 3256
BstC8I	GCNNGC	7 cut(s)	216, 289, 468, 493, 1228, 2144, 2257
BstDSI	CCRYGG	1 cut(s)	2043
BstEII	GGTNACC	1 cut(s)	2134
BstENI	CCTNNNNNAGG	1 cut(s)	2982
BstF5I	GGATG	5 cut(s)	403, 1259, 2189, 2650, 2994
BstHHI	GCGC	1 cut(s)	403
BstMAI	GTCTC	3 cut(s)	260, 1871, 2660
BstMWI	GCNNNNNNNGC	8 cut(s)	176, 281, 407, 1417, 2863, 3176, 3386, 3415
BstNI	CCWGG	7 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979
BstNSI	RCATGY	2 cut(s)	291, 3211
BstPAI	GACNNNNGTC	1 cut(s)	756
BstPI	GGTNACC	1 cut(s)	2134
BstSCI	CCNGG	8 cut(s)	196, 480, 1043, 1243, 1753, 2355, 2977, 3104
BstSFI	CTRYAG	6 cut(s)	171, 270, 396, 534, 852, 2178
BstSLI	GKGCMC	2 cut(s)	1171, 3375
BstV1I	GCAGC	8 cut(s)	296, 410, 535, 2400, 3182, 3185, 3188, 3402
BstV2I	GAAGAC	4 cut(s)	656, 785, 1067, 2749
BstX2I	RGATCY	9 cut(s)	385, 1489, 1658, 2172, 2423, 2581, 2937, 3241, 3347
BstXI	CCANNNNNNTGG	1 cut(s)	2044
BstYI	RGATCY	9 cut(s)	385, 1489, 1658, 2172, 2423, 2581, 2937, 3241, 3347
BstZ17I	GTATAC	1 cut(s)	711
BsuI	GTATCC	2 cut(s)	2055, 3129
BsuRI	GGCC	5 cut(s)	1043, 1354, 2036, 2982, 3292
BtgI	CCRYGG	1 cut(s)	2043
BtsCI	GGATG	5 cut(s)	403, 1259, 2189, 2650, 2994
BtsI	GCAGTG	1 cut(s)	3293
BtsIMutI	CAGTG	7 cut(s)	1027, 1394, 2595, 2682, 2917, 3079, 3293
BveI	ACCTGC	3 cut(s)	175, 845, 1502
Cac8I	GCNNGC	7 cut(s)	216, 289, 468, 493, 1228, 2144, 2257
CaiI	CAGNNNCTG	2 cut(s)	127, 3415
CciI	TCATGA	2 cut(s)	1638, 2242
CfoI	GCGC	1 cut(s)	403
Cfr10I	RCCGGY	1 cut(s)	2119
Cfr13I	GGNCC	1 cut(s)	2034
CsiI	ACCWGGT	1 cut(s)	1753
Csp6I	GTAC	5 cut(s)	85, 1975, 3128, 3233, 3423
CspAI	ACCGGT	1 cut(s)	2119
CviQI	GTAC	5 cut(s)	85, 1975, 3128, 3233, 3423
DraI	TTTAAA	1 cut(s)	1690
DraIII	CACNNNGTG	2 cut(s)	1327, 1757
EaeI	YGGCCR	3 cut(s)	1352, 2980, 3290
Eam1104I	CTCTTC	6 cut(s)	258, 417, 1779, 1919, 2484, 2928
EarI	CTCTTC	6 cut(s)	258, 417, 1779, 1919, 2484, 2928
Ecl136II	GAGCTC	1 cut(s)	1420
Eco130I	CCWWGG	5 cut(s)	2043, 2502, 2554, 2718, 2983
Eco147I	AGGCCT	1 cut(s)	1043
Eco24I	GRGCYC	1 cut(s)	1422
Eco32I	GATATC	1 cut(s)	785
Eco53kI	GAGCTC	1 cut(s)	1420
Eco57I	CTGAAG	3 cut(s)	1287, 2411, 3431
Eco72I	CACGTG	1 cut(s)	1906
Eco88I	CYCGRG	1 cut(s)	2276
Eco91I	GGTNACC	1 cut(s)	2134
EcoICRI	GAGCTC	1 cut(s)	1420
EcoNI	CCTNNNNNAGG	1 cut(s)	2982
EcoO65I	GGTNACC	1 cut(s)	2134
EcoRII	CCWGG	7 cut(s)	196, 480, 1043, 1243, 1753, 2355, 2977
EcoRV	GATATC	1 cut(s)	785
EcoT14I	CCWWGG	5 cut(s)	2043, 2502, 2554, 2718, 2983
EcoT38I	GRGCYC	1 cut(s)	1422
ErhI	CCWWGG	5 cut(s)	2043, 2502, 2554, 2718, 2983
FalI	AAGNNNNNCTT	4 cut(s)	720, 752, 759, 791
FaqI	GGGAC	1 cut(s)	406
FauNDI	CATATG	4 cut(s)	626, 1794, 2874, 2906
FbaI	TGATCA	1 cut(s)	937
FblI	GTMKAC	2 cut(s)	225, 710
FokI	GGATG	5 cut(s)	390, 1246, 2196, 2657, 3001
FriOI	GRGCYC	1 cut(s)	1422
FspBI	CTAG	7 cut(s)	17, 788, 1781, 1832, 1964, 2589, 2930
GlaI	GCGC	1 cut(s)	402
GsuI	CTGGAG	3 cut(s)	219, 1066, 1934
HaeIII	GGCC	5 cut(s)	1043, 1354, 2036, 2982, 3292
HapII	CCGG	5 cut(s)	617, 2120, 2642, 2732, 3105
HhaI	GCGC	1 cut(s)	403
Hin6I	GCGC	1 cut(s)	401
HinP1I	GCGC	1 cut(s)	401
HincII	GTYRAC	3 cut(s)	676, 1275, 2571
HindII	GTYRAC	3 cut(s)	676, 1275, 2571
HindIII	AAGCTT	4 cut(s)	177, 953, 2156, 3259
HpaI	GTTAAC	1 cut(s)	676
HpaII	CCGG	5 cut(s)	617, 2120, 2642, 2732, 3105
HphI	GGTGA	7 cut(s)	172, 1190, 1743, 2420, 2790, 2949, 3197
Hpy99I	CGWCG	1 cut(s)	2485
HpyCH4IV	ACGT	4 cut(s)	838, 1654, 1905, 2440
HpyF10VI	GCNNNNNNNGC	8 cut(s)	176, 281, 407, 1417, 2863, 3176, 3386, 3415
HpySE526I	ACGT	4 cut(s)	838, 1654, 1905, 2440
HspAI	GCGC	1 cut(s)	401
Ksp22I	TGATCA	1 cut(s)	937
KspAI	GTTAAC	1 cut(s)	676
LguI	GCTCTTC	1 cut(s)	417
LmnI	GCTCC	9 cut(s)	500, 1047, 1417, 1456, 2014, 2191, 2531, 2997, 3412
Lsp1109I	GCAGC	8 cut(s)	296, 410, 535, 2400, 3182, 3185, 3188, 3402
LweI	GCATC	6 cut(s)	112, 412, 808, 1424, 2242, 2979
MabI	ACCWGGT	1 cut(s)	1753
MaeI	CTAG	7 cut(s)	17, 788, 1781, 1832, 1964, 2589, 2930
MaeII	ACGT	4 cut(s)	838, 1654, 1905, 2440
MaeIII	GTNAC	7 cut(s)	961, 1438, 1759, 2134, 2843, 3068, 3433
MfeI	CAATTG	3 cut(s)	1143, 1852, 1865
MflI	RGATCY	9 cut(s)	385, 1489, 1658, 2172, 2423, 2581, 2937, 3241, 3347
MhlI	GDGCHC	5 cut(s)	1171, 1422, 2019, 3090, 3375
MlsI	TGGCCA	3 cut(s)	1354, 2982, 3292
MluNI	TGGCCA	3 cut(s)	1354, 2982, 3292
MlyI	GAGTC	2 cut(s)	1270, 2566
MmeI	TCCRAC	3 cut(s)	1141, 2466, 2610
Mox20I	TGGCCA	3 cut(s)	1354, 2982, 3292
MroXI	GAANNNNTTC	5 cut(s)	254, 1239, 1928, 2109, 3262
MscI	TGGCCA	3 cut(s)	1354, 2982, 3292
MseI	TTAA	7 cut(s)	675, 726, 1299, 1430, 1689, 2169, 2771
MslI	CAYNNNNRTG	3 cut(s)	158, 2340, 2688
Msp20I	TGGCCA	3 cut(s)	1354, 2982, 3292
MspA1I	CMGCKG	1 cut(s)	3220
MspI	CCGG	5 cut(s)	617, 2120, 2642, 2732, 3105
MspR9I	CCNGG	8 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979, 3106
MunI	CAATTG	3 cut(s)	1143, 1852, 1865
Mva1269I	GAATGC	2 cut(s)	1207, 3357
MvaI	CCWGG	7 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979
MwoI	GCNNNNNNNGC	8 cut(s)	176, 281, 407, 1417, 2863, 3176, 3386, 3415
NciI	CCSGG	1 cut(s)	3106
NcoI	CCATGG	1 cut(s)	2043
NdeI	CATATG	4 cut(s)	626, 1794, 2874, 2906
NlaIV	GGNNCC	5 cut(s)	490, 859, 1458, 2865, 3349
NmuCI	GTSAC	1 cut(s)	1759
NspI	RCATGY	2 cut(s)	291, 3211
NspV	TTCGAA	2 cut(s)	182, 3256
PaeI	GCATGC	1 cut(s)	291
PaeR7I	CTCGAG	1 cut(s)	2276
PagI	TCATGA	2 cut(s)	1638, 2242
PaqCI	CACCTGC	1 cut(s)	845
PceI	AGGCCT	1 cut(s)	1043
PciI	ACATGT	1 cut(s)	3207
PciSI	GCTCTTC	1 cut(s)	417
PcsI	WCGNNNNNNNCGW	1 cut(s)	566
PctI	GAATGC	2 cut(s)	1207, 3357
PdmI	GAANNNNTTC	5 cut(s)	254, 1239, 1928, 2109, 3262
PfeI	GAWTC	9 cut(s)	455, 557, 763, 928, 1082, 1929, 2288, 2804, 3040
PflMI	CCANNNNNTGG	2 cut(s)	2049, 3368
PfoI	TCCNGGA	4 cut(s)	196, 480, 1243, 2355
PinAI	ACCGGT	1 cut(s)	2119
PleI	GAGTC	2 cut(s)	1270, 2566
PmaCI	CACGTG	1 cut(s)	1906
PmlI	CACGTG	1 cut(s)	1906
PpsI	GAGTC	2 cut(s)	1270, 2566
Ppu21I	YACGTR	3 cut(s)	1655, 1906, 2441
PscI	ACATGT	1 cut(s)	3207
PshAI	GACNNNNGTC	1 cut(s)	756
Psp124BI	GAGCTC	1 cut(s)	1422
Psp6I	CCWGG	7 cut(s)	196, 480, 1043, 1243, 1753, 2355, 2977
PspCI	CACGTG	1 cut(s)	1906
PspEI	GGTNACC	1 cut(s)	2134
PspGI	CCWGG	7 cut(s)	196, 480, 1043, 1243, 1753, 2355, 2977
PspN4I	GGNNCC	5 cut(s)	490, 859, 1458, 2865, 3349
PspPI	GGNCC	1 cut(s)	2034
PspXI	VCTCGAGB	1 cut(s)	2276
PstI	CTGCAG	2 cut(s)	400, 856
PstNI	CAGNNNCTG	2 cut(s)	127, 3415
PsuI	RGATCY	9 cut(s)	385, 1489, 1658, 2172, 2423, 2581, 2937, 3241, 3347
PvuII	CAGCTG	1 cut(s)	3220
RsaI	GTAC	5 cut(s)	86, 1976, 3129, 3234, 3424
RsaNI	GTAC	5 cut(s)	85, 1975, 3128, 3233, 3423
RseI	CAYNNNNRTG	3 cut(s)	158, 2340, 2688
SacI	GAGCTC	1 cut(s)	1422
SapI	GCTCTTC	1 cut(s)	417
SaqAI	TTAA	7 cut(s)	675, 726, 1299, 1430, 1689, 2169, 2771
Sau96I	GGNCC	1 cut(s)	2034
ScaI	AGTACT	1 cut(s)	3129
SchI	GAGTC	2 cut(s)	1270, 2566
ScrFI	CCNGG	8 cut(s)	198, 482, 1045, 1245, 1755, 2357, 2979, 3106
SduI	GDGCHC	5 cut(s)	1171, 1422, 2019, 3090, 3375
SexAI	ACCWGGT	1 cut(s)	1753
SfaNI	GCATC	6 cut(s)	112, 412, 808, 1424, 2242, 2979
SfcI	CTRYAG	6 cut(s)	171, 270, 396, 534, 852, 2178
Sfr274I	CTCGAG	1 cut(s)	2276
SfuI	TTCGAA	2 cut(s)	182, 3256
SlaI	CTCGAG	1 cut(s)	2276
SmiMI	CAYNNNNRTG	3 cut(s)	158, 2340, 2688
SmlI	CTYRAG	5 cut(s)	1493, 1739, 2233, 2276, 3236
SmoI	CTYRAG	5 cut(s)	1493, 1739, 2233, 2276, 3236
SphI	GCATGC	1 cut(s)	291
SseBI	AGGCCT	1 cut(s)	1043
SsiI	CCGC	5 cut(s)	61, 630, 1460, 1943, 3142
SspI	AATATT	4 cut(s)	10, 446, 547, 879
SspMI	CTAG	7 cut(s)	17, 788, 1781, 1832, 1964, 2589, 2930
SstI	GAGCTC	1 cut(s)	1422
StuI	AGGCCT	1 cut(s)	1043
StyD4I	CCNGG	8 cut(s)	196, 480, 1043, 1243, 1753, 2355, 2977, 3104
StyI	CCWWGG	5 cut(s)	2043, 2502, 2554, 2718, 2983
TaiI	ACGT	4 cut(s)	841, 1657, 1908, 2443
TaqI	TCGA	8 cut(s)	182, 560, 569, 781, 893, 1724, 2277, 3256
TatI	WGTACW	3 cut(s)	84, 1974, 3127
TauI	GCSGC	3 cut(s)	64, 633, 1462
TfiI	GAWTC	9 cut(s)	455, 557, 763, 928, 1082, 1929, 2288, 2804, 3040
Tru1I	TTAA	7 cut(s)	675, 726, 1299, 1430, 1689, 2169, 2771
Tru9I	TTAA	7 cut(s)	675, 726, 1299, 1430, 1689, 2169, 2771
TscAI	CASTG	7 cut(s)	1027, 1401, 2602, 2682, 2924, 3079, 3300
TseFI	GTSAC	1 cut(s)	1759
TseI	GCWGC	8 cut(s)	284, 398, 523, 2413, 3170, 3173, 3176, 3415
Tsp45I	GTSAC	1 cut(s)	1759
TspDTI	ATGAA	8 cut(s)	276, 786, 1748, 2231, 2393, 2893, 3344, 3372
TspRI	CASTG	7 cut(s)	1027, 1401, 2602, 2682, 2924, 3079, 3300
Van91I	CCANNNNNTGG	2 cut(s)	2049, 3368
XagI	CCTNNNNNAGG	1 cut(s)	2982
XapI	RAATTY	6 cut(s)	428, 516, 1009, 1693, 1993, 2822
XceI	RCATGY	2 cut(s)	291, 3211
XcmI	CCANNNNNNNNNTGG	1 cut(s)	912
XhoI	CTCGAG	1 cut(s)	2276
XmiI	GTMKAC	2 cut(s)	225, 710
XmnI	GAANNNNTTC	5 cut(s)	254, 1239, 1928, 2109, 3262
XspI	CTAG	7 cut(s)	17, 788, 1781, 1832, 1964, 2589, 2930
ZrmI	AGTACT	1 cut(s)	3129

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.