Rroxscaffold_7G00187370

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000203
Physical Location & Seq
Reverse (-)
26797589 .. 26802589
5001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187370.1

Sequence Viewer

Length: 3180 bp
ATGGAAGCTATCGTTTCATCAGTTCTTGGAAAACTAGCAGAGTACACAGTTGAACCGGTTGTGCGACAGTTTGGTTATCTGATTCACTATGAGAGTAACGTTGCTCGTCTCAACAACCATGTTGAGAGGCTGAATGCCCAAAGACGCGACGTGGCAGAAAAAATTGAAGCAGCGACCAGGAATTCAGAGACTATTCTTCACGAAGTTGAGATCTGGTTGGACCAGGTGGATCAAACCGTACGAGACAAGGATACATGTTTTGAAAACGAGCGAACAGCAAAGGCGGAATGCTGCTCAAATGGCTGGTTCCCCAATTTGAAGATACGGCATTCCTTGAGTAGGAAAGCGAAGAAGATGACTCGGGATGTCGATAGCCTCATTGATAACCATTGTAAAATCTTGGTAACATCACGAAACCAAGATATCTTCAATGATATGGAAGAAGCTAGAAAGAAATTCCCCATTGACGTTTTGCCCGAAAGTGATGCATGGAGTCTGTTTAAGAAGATGGCTGGAGATGATATTGAATCTGATCCCGAGCTGCGTTCGATAGCAGAACAGGTACTCGAGAAATGTGATGGATTACCAGTTGCAATTTCGACCCTGGGAAGCGCACTACGGCGGAAAACGAAAGCGGTCTGGAATGATGCACTCAGACTATTACAAAAGCCTTTCGGGGGAGATATTCAAGGAATGAAGAAGAACGTGTATCAGTCAATCAGGTTGAGTTACGACTTTCTGGAAAGAGAGGAGGCCAAATCCTGTTTTCTGTTGTGTTGCATATTTCGAGAAAGCACTAATATATGTGTTGAAGATTTGGTGACGCATGGATTTGGTCTTGGGGTCTTACATAGAATTGATTCAGTTGAAGGGGGAAGGGATCGTGTAGAAGCCTTGGTTGACACACTCAAAAGTTGCTATTTATTGTTGGACAGTGACAAAGAAGATTGTGTAAGAATGCATGACGTGGTGCGTGCCGTAGGCTTACATATAGCCTCTGCTGAGAGTGAAGGCACATTTCTTATAAGACACGGAGTGGCATCGGAGAATTGGACCAGGGGATTCATTTGTACATCAATGACCGCCAATAAAAGTACTCATGAGCTTTTGCTGTTGTCATGCACAGATGATGAAAAGAGGTCCGATGTGGACACACTAGGCACATTGCACGGAGTGGAAGATCTTAAGGTCTTGGACATCTCAGTTCCTTATAAGGGTCCTTATACATGGATAACGTTTGCTATTATGTCTAGAACAAGATTGGAATCACTTCCGGTACTTATGAGAAACATTCAAACACTCTGTCTAAGGGGTTTAGAACTCAAATTGGAAACAATTTCTATGATTGGGGACCTACGGACACTCACAATACTCCGCTTAGGTGGATCTTCCATACAGCATGTACCTGAGGAATTTAAAAACTTGTGCAATCTAAAGTTGCTAAATTTGGCGGATTGTTCGGAACTTCAAGAAATCTCACCAGGTGTCATATCAAGTTTAACCAAACTAGAAGAATTGTACTTGTGGGGTAGCTTCGAGGACTGGAATGATGTGCGGATTATTCAACTTTCATCGGAGCTACTGCAGTTGCCTTTGTTGACGAGTTTAGAAACCACTCTGTCTGTTGATATTCTGCAAAACAGCAGGCATCTATTTGCTAAGCTTAAAATATTTAGGCTCTGTATACTAAAAAATAAAAGACGTGTACCCAAGTTTCTCCGCAGTATACCACTTTACAAATTGCCTGCAAATCCTCGTGACAACCTCTTGAGACTTGAAGAGCTTGGCGCAGACTGCTCTCTTCTGGAAGTCTCTGTGTTGCTAAAGACAACTCATTTTCTACACCTAGGAAATGAAACTTTGGCTCACCCTTTCAATATAGTAGATGCAGAGTGCGGTGGAAACTTGAAGGGTCTCACGGTCACAGACTGTGATGCTCTGGAATATCTCGTTGACTTGACATGTGCTGAAACTACTCCTGTAGTCTTTCCTGTCTTGGCTTCACTGGAGATCTATGGTGCACAGAGGCTGAAGGAGATTTTTCATGGATTACTGCCAGTGGGGTCGCTTAAATCACTACGACGTTTAATCATACACCGGGCGCATGAATTAAGGCATGTTTGGAAGACAGCAAGCCAGTTTGACCGCCTGGAAAGTCTAACATTAGCCGATTGTCAATCGTTGCAAGTTATTGTTGCTTGGCAAGAATCGGGACATCATCATGAGGAAGAAGCCAAGGAGATCAACTTCCCTAATTTGACACAGTTGGAACTTGCAGAACTACCAAGCCTCGTTGGGATATGTAGCAAAAAAGTCTGCAAGCTCAACTTCTCTAAATTGTTAGATTTACAACTCAGAACGCTTCCGCGATTCAGTGGTCAGTGGTTGAATGGTTTGGCTCCAACAAGTAACTCTAATGACGTCGATGTACAACATCTATTCGACCCCAAGGTTGATTTTCCACTCTTGAGACTTCTGTATCTTGAACATATGCACAACTTTGAAGAGATATGGCACAACCAACTCTCTCCCTACTCATTCTGCGAACTAAGAGAACTCCACGTGAGACATTGTGACAAAATATCACGTTTGGTACCAACATACATGCAAAACAGATTGCAGAAATTGGAGAAGATACATGTGTCCAAGTGTTCTTCGCTACAAGAGATTTTTGAATTGAGGAGATCGAGGATTGAAGGATCAAGGGAAACACCGTCAAATATTTACCAACCTGATCAAGGAATACTACAAATAAACAATCAGATCAAATTCTTTCAGCATCTAACAAATTTATCCGTCGATGGATGTGAAAGTTTGAGAAAATTGTGCTGTTCTTCCATTGCAAGAAGTCTTGTGAACCTCGAAAAACTAGAAATCAGTCAGTGCAACAGGTTGGGAGAAATAATAGCAGCAGAAGAGGGTGAAGAAGCGGAGGATAACAGCATGTTCCATCTCAGACTTTTGACCCTTCAATATCTACCAAATCTCGTAAGTTTTTTCAGAGAAGAATGTGCTTTATATTGGCCATCCATGGAAGTCATAACTATTAAGGACTGCCCCGAAATGAAGAAATTCTGTTCAGGATCGCTTATCACATCAATGGAAATGAAAATAGAAATAAGTGGTAAAGTTGATGAGAATATTCACAGGGAGCTTGACATGAGTAAGAGGAAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1059

Amino Acids

120.79

Weight (kDa)

5.91

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 447 - 747 3.4e-06 Leucine-rich repeat region
LRR_8 PF13855 453 - 508 1.9e-06 Leucine rich repeat
LRR_RPS2 PF23247 675 - 784 5e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 828 - 897 3.8e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 920 - 1034 3.6e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1025, 1212
AatII GACGTC 1 cut(s) 2424
Acc65I GGTACC 1 cut(s) 2593
AccB1I GGYRCC 1 cut(s) 2593
AccI GTMKAC 2 cut(s) 1684, 1726
AccII CGCG 2 cut(s) 147, 2368
AclI AACGTT 2 cut(s) 99, 1235
AclWI GGATC 6 cut(s) 237, 527, 888, 1393, 2707, 3091
AcoI YGGCCR 1 cut(s) 3023
AcsI RAATTY 7 cut(s) 181, 455, 1412, 1444, 2768, 2788, 3071
AcuI CTGAAG 1 cut(s) 2051
AcvI CACGTG 1 cut(s) 2563
AcyI GRCGYC 1 cut(s) 2421
AdeI CACNNNGTG 3 cut(s) 1036, 1174, 1484
AfiI CCNNNNNNNGG 4 cut(s) 339, 677, 1214, 2738
AflII CTTAAG 1 cut(s) 1184
AflIII ACRYGT 5 cut(s) 254, 705, 1702, 1961, 2638
AgeI ACCGGT 1 cut(s) 55
AjiI CACGTC 3 cut(s) 151, 967, 1703
AjnI CCWGG 6 cut(s) 176, 222, 603, 1055, 1480, 2148
Alw21I GWGCWC 1 cut(s) 2023
Alw26I GTCTC 8 cut(s) 113, 182, 237, 1765, 1816, 1919, 2464, 2560
Alw44I GTGCAC 1 cut(s) 2019
AlwI GGATC 6 cut(s) 237, 527, 888, 1393, 2707, 3091
AlwNI CAGNNNCTG 1 cut(s) 2029
Ama87I CYCGRG 3 cut(s) 360, 536, 566
AoxI GGCC 3 cut(s) 753, 3023, 3174
ApaLI GTGCAC 1 cut(s) 2019
ApeKI GCWGC 4 cut(s) 170, 291, 541, 2909
ApoI RAATTY 7 cut(s) 181, 455, 1412, 1444, 2768, 2788, 3071
ArsI GACNNNNNNTTYG 2 cut(s) 1288, 1320
AsiGI ACCGGT 1 cut(s) 55
Asp700I GAANNNNTTC 3 cut(s) 859, 1269, 3071
Asp718I GGTACC 1 cut(s) 2593
AspA2I CCTAGG 1 cut(s) 1846
AspLEI GCGC 3 cut(s) 614, 1790, 2104
AspS9I GGNCC 5 cut(s) 220, 1053, 1140, 1217, 1351
AsuC2I CCSGG 1 cut(s) 2099
AsuHPI GGTGA 4 cut(s) 832, 1470, 1859, 2933
AvaI CYCGRG 3 cut(s) 360, 536, 566
AvaII GGWCC 5 cut(s) 220, 1053, 1140, 1217, 1351
AvrII CCTAGG 1 cut(s) 1846
AxyI CCTNAGG 1 cut(s) 1407
BaeGI GKGCMC 1 cut(s) 2023
BaeI ACNNNNGTAYC 4 cut(s) 2462, 2495, 2627, 2660
BalI TGGCCA 1 cut(s) 3025
BanI GGYRCC 1 cut(s) 2593
BauI CACGAG 1 cut(s) 1755
BbrPI CACGTG 1 cut(s) 2563
BbsI GAAGAC 1 cut(s) 2132
Bbv12I GWGCWC 1 cut(s) 2023
BbvI GCAGC 4 cut(s) 182, 278, 528, 2921
BccI CCATC 5 cut(s) 502, 572, 2795, 2958, 3034
BceAI ACGGC 3 cut(s) 341, 635, 962
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 6 cut(s) 178, 224, 605, 1057, 1482, 2150
BciVI GTATCC 1 cut(s) 244
BclI TGATCA 1 cut(s) 2734
BcnI CCSGG 1 cut(s) 2099
BcoDI GTCTC 8 cut(s) 113, 182, 237, 1765, 1816, 1919, 2464, 2560
BfaI CTAG 7 cut(s) 35, 447, 1157, 1251, 1508, 1847, 2870
BfmI CTRYAG 2 cut(s) 1583, 1980
BfrI CTTAAG 1 cut(s) 1184
BfuI GTATCC 1 cut(s) 244
BglII AGATCT 3 cut(s) 210, 1180, 2010
BisI GCNGC 4 cut(s) 171, 292, 542, 2910
BlnI CCTAGG 1 cut(s) 1846
BlpI GCTNAGC 1 cut(s) 1659
BlsI GCNGC 4 cut(s) 172, 293, 543, 2911
BmcAI AGTACT 1 cut(s) 1096
Bme1390I CCNGG 7 cut(s) 178, 224, 605, 1057, 1482, 2099, 2150
Bme18I GGWCC 5 cut(s) 220, 1053, 1140, 1217, 1351
BmeT110I CYCGRG 3 cut(s) 360, 536, 566
BmgBI CACGTC 3 cut(s) 151, 967, 1703
BmgT120I GGNCC 5 cut(s) 220, 1053, 1140, 1217, 1351
BmiI GGNNCC 5 cut(s) 308, 1218, 1352, 2400, 2595
BmrFI CCNGG 7 cut(s) 178, 224, 605, 1057, 1482, 2099, 2150
BmsI GCATC 7 cut(s) 475, 637, 1049, 1657, 1876, 1924, 2788
BpiI GAAGAC 1 cut(s) 2132
BpmI CTGGAG 2 cut(s) 534, 2027
Bpu10I CCTNAGC 1 cut(s) 1378
Bpu1102I GCTNAGC 1 cut(s) 1659
BpuEI CTTGAG 3 cut(s) 355, 1789, 2488
BpuMI CCSGG 1 cut(s) 2099
BsaAI YACGTR 1 cut(s) 2563
BsaBI GATNNNNATC 1 cut(s) 1264
BsaHI GRCGYC 1 cut(s) 2421
BsaI GGTCTC 1 cut(s) 1919
BsaJI CCNNGG 8 cut(s) 603, 604, 894, 1056, 1846, 2235, 2448, 3030
BsaWI WCCGGW 2 cut(s) 55, 1273
Bsc4I CCNNNNNNNGG 4 cut(s) 339, 677, 1214, 2738
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 5 cut(s) 587, 1547, 2010, 2057, 2137
Bse21I CCTNAGG 1 cut(s) 1407
Bse3DI GCAATG 2 cut(s) 1163, 2838
Bse8I GATNNNNATC 1 cut(s) 1264
BseBI CCWGG 6 cut(s) 178, 224, 605, 1057, 1482, 2150
BseDI CCNNGG 8 cut(s) 603, 604, 894, 1056, 1846, 2235, 2448, 3030
BseGI GGATG 3 cut(s) 370, 2810, 3026
BseJI GATNNNNATC 1 cut(s) 1264
BseLI CCNNNNNNNGG 4 cut(s) 339, 677, 1214, 2738
BseMI GCAATG 2 cut(s) 1163, 2838
BseMII CTCAG 6 cut(s) 667, 993, 1215, 1398, 2368, 2968
BseNI ACTGG 5 cut(s) 587, 1547, 2010, 2057, 2137
BseRI GAGGAG 2 cut(s) 764, 2695
BseSI GKGCMC 1 cut(s) 2023
BseXI GCAGC 4 cut(s) 182, 278, 528, 2921
Bsh1236I CGCG 2 cut(s) 147, 2368
BshFI GGCC 3 cut(s) 755, 3025, 3176
BshNI GGYRCC 1 cut(s) 2593
BshTI ACCGGT 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 2023
BsiHKCI CYCGRG 3 cut(s) 360, 536, 566
BsiSI CCGG 3 cut(s) 56, 1274, 2098
BsiWI CGTACG 1 cut(s) 238
BslFI GGGAC 2 cut(s) 1364, 2226
BslI CCNNNNNNNGG 4 cut(s) 339, 677, 1214, 2738
BsmAI GTCTC 8 cut(s) 113, 182, 237, 1765, 1816, 1919, 2464, 2560
BsmBI CGTCTC 1 cut(s) 113
BsmFI GGGAC 2 cut(s) 1364, 2226
BsmI GAATGC 4 cut(s) 139, 293, 328, 963
BsnI GGCC 3 cut(s) 755, 3025, 3176
Bso31I GGTCTC 1 cut(s) 1919
BsoBI CYCGRG 3 cut(s) 360, 536, 566
Bsp1286I GDGCHC 1 cut(s) 2023
Bsp1407I TGTACA 2 cut(s) 1070, 2428
Bsp1720I GCTNAGC 1 cut(s) 1659
Bsp19I CCATGG 1 cut(s) 3030
BspANI GGCC 3 cut(s) 755, 3025, 3176
BspCNI CTCAG 6 cut(s) 666, 994, 1214, 1399, 2367, 2967
BspFNI CGCG 2 cut(s) 147, 2368
BspHI TCATGA 2 cut(s) 1099, 2221
BspLI GGNNCC 5 cut(s) 308, 1218, 1352, 2400, 2595
BspMAI CTGCAG 1 cut(s) 1587
BspPI GGATC 6 cut(s) 237, 527, 888, 1393, 2707, 3091
BspQI GCTCTTC 1 cut(s) 1773
BspT107I GGYRCC 1 cut(s) 2593
BspTI CTTAAG 1 cut(s) 1184
BspTNI GGTCTC 1 cut(s) 1919
BsrDI GCAATG 2 cut(s) 1163, 2838
BsrFI RCCGGY 1 cut(s) 55
BsrGI TGTACA 2 cut(s) 1070, 2428
BsrI ACTGG 5 cut(s) 587, 1547, 2010, 2057, 2137
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 8 cut(s) 603, 604, 894, 1056, 1846, 2235, 2448, 3030
BssNAI GTATAC 2 cut(s) 1685, 1727
BssNI GRCGYC 1 cut(s) 2421
BssSI CACGAG 1 cut(s) 1755
BssT1I CCWWGG 5 cut(s) 894, 1846, 2235, 2448, 3030
Bst1107I GTATAC 2 cut(s) 1685, 1727
Bst2BI CACGAG 1 cut(s) 1755
Bst2UI CCWGG 6 cut(s) 178, 224, 605, 1057, 1482, 2150
Bst4CI ACNGT 8 cut(s) 49, 69, 238, 935, 1921, 1931, 2265, 2715
Bst6I CTCTTC 4 cut(s) 1773, 1806, 2499, 2910
BstACI GRCGYC 1 cut(s) 2421
BstAFI CTTAAG 1 cut(s) 1184
BstAUI TGTACA 2 cut(s) 1070, 2428
BstBAI YACGTR 1 cut(s) 2563
BstC8I GCNNGC 5 cut(s) 975, 1646, 1746, 2134, 2321
BstDSI CCRYGG 1 cut(s) 3030
BstENI CCTNNNNNAGG 2 cut(s) 337, 2736
BstF5I GGATG 3 cut(s) 370, 2810, 3026
BstFNI CGCG 2 cut(s) 147, 2368
BstHHI GCGC 3 cut(s) 614, 1790, 2104
BstMAI GTCTC 8 cut(s) 113, 182, 237, 1765, 1816, 1919, 2464, 2560
BstMWI GCNNNNNNNGC 2 cut(s) 300, 1794
BstNI CCWGG 6 cut(s) 178, 224, 605, 1057, 1482, 2150
BstNSI RCATGY 7 cut(s) 258, 1403, 1965, 2120, 2608, 2642, 2947
BstSCI CCNGG 7 cut(s) 176, 222, 603, 1055, 1480, 2097, 2148
BstSFI CTRYAG 2 cut(s) 1583, 1980
BstSLI GKGCMC 1 cut(s) 2023
BstUI CGCG 2 cut(s) 147, 2368
BstV1I GCAGC 4 cut(s) 182, 278, 528, 2921
BstV2I GAAGAC 1 cut(s) 2132
BstX2I RGATCY 4 cut(s) 210, 1180, 1385, 2010
BstYI RGATCY 4 cut(s) 210, 1180, 1385, 2010
BstZ17I GTATAC 2 cut(s) 1685, 1727
Bsu36I CCTNAGG 1 cut(s) 1407
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 3 cut(s) 755, 3025, 3176
BtgI CCRYGG 1 cut(s) 3030
BtrI CACGTC 3 cut(s) 151, 967, 1703
BtsCI GGATG 3 cut(s) 370, 2810, 3026
BtsIMutI CAGTG 6 cut(s) 940, 2003, 2064, 2380, 2387, 2888
Cac8I GCNNGC 5 cut(s) 975, 1646, 1746, 2134, 2321
CaiI CAGNNNCTG 1 cut(s) 2029
CciI TCATGA 2 cut(s) 1099, 2221
CfoI GCGC 3 cut(s) 614, 1790, 2104
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 5 cut(s) 220, 1053, 1140, 1217, 1351
CseI GACGC 2 cut(s) 153, 832
CsiI ACCWGGT 2 cut(s) 222, 1480
CspAI ACCGGT 1 cut(s) 55
DraI TTTAAA 1 cut(s) 1417
DraIII CACNNNGTG 3 cut(s) 1036, 1174, 1484
EaeI YGGCCR 1 cut(s) 3023
Eam1104I CTCTTC 4 cut(s) 1773, 1806, 2499, 2910
EarI CTCTTC 4 cut(s) 1773, 1806, 2499, 2910
EciI GGCGGA 3 cut(s) 299, 637, 1466
Eco130I CCWWGG 5 cut(s) 894, 1846, 2235, 2448, 3030
Eco147I AGGCCT 1 cut(s) 3176
Eco31I GGTCTC 1 cut(s) 1919
Eco32I GATATC 1 cut(s) 424
Eco47I GGWCC 5 cut(s) 220, 1053, 1140, 1217, 1351
Eco57I CTGAAG 1 cut(s) 2051
Eco72I CACGTG 1 cut(s) 2563
Eco81I CCTNAGG 1 cut(s) 1407
Eco88I CYCGRG 3 cut(s) 360, 536, 566
EcoNI CCTNNNNNAGG 2 cut(s) 337, 2736
EcoO109I RGGNCCY 2 cut(s) 1217, 1351
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 6 cut(s) 176, 222, 603, 1055, 1480, 2148
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 5 cut(s) 894, 1846, 2235, 2448, 3030
EcoT22I ATGCAT 2 cut(s) 490, 963
ErhI CCWWGG 5 cut(s) 894, 1846, 2235, 2448, 3030
Esp3I CGTCTC 1 cut(s) 113
FalI AAGNNNNNCTT 2 cut(s) 2312, 2344
FaqI GGGAC 2 cut(s) 1364, 2226
FauNDI CATATG 1 cut(s) 2490
FbaI TGATCA 1 cut(s) 2734
FblI GTMKAC 2 cut(s) 1684, 1726
Fnu4HI GCNGC 4 cut(s) 171, 292, 542, 2910
FokI GGATG 3 cut(s) 377, 2817, 3013
Fsp4HI GCNGC 4 cut(s) 171, 292, 542, 2910
FspBI CTAG 7 cut(s) 35, 447, 1157, 1251, 1508, 1847, 2870
GlaI GCGC 3 cut(s) 613, 1789, 2103
GluI GCNGC 4 cut(s) 171, 292, 542, 2910
GsuI CTGGAG 2 cut(s) 534, 2027
HaeIII GGCC 3 cut(s) 755, 3025, 3176
HapII CCGG 3 cut(s) 56, 1274, 2098
HgaI GACGC 2 cut(s) 153, 832
HhaI GCGC 3 cut(s) 614, 1790, 2104
Hin1I GRCGYC 1 cut(s) 2421
Hin6I GCGC 3 cut(s) 612, 1788, 2102
HinP1I GCGC 3 cut(s) 612, 1788, 2102
HincII GTYRAC 3 cut(s) 901, 1599, 1954
HindII GTYRAC 3 cut(s) 901, 1599, 1954
HindIII AAGCTT 1 cut(s) 1661
HinfI GANTC 9 cut(s) 82, 358, 493, 527, 860, 1062, 1265, 2207, 2370
HpaII CCGG 3 cut(s) 56, 1274, 2098
HphI GGTGA 4 cut(s) 832, 1470, 1859, 2933
Hpy99I CGWCG 4 cut(s) 152, 2085, 2426, 2801
HpyAV CCTTC 8 cut(s) 863, 870, 1004, 1903, 2026, 2690, 2978, 3166
HpyCH4III ACNGT 8 cut(s) 49, 69, 238, 935, 1921, 1931, 2265, 2715
HpyF10VI GCNNNNNNNGC 2 cut(s) 300, 1794
Hsp92I GRCGYC 1 cut(s) 2421
HspAI GCGC 3 cut(s) 612, 1788, 2102
KpnI GGTACC 1 cut(s) 2597
Ksp22I TGATCA 1 cut(s) 2734
LguI GCTCTTC 1 cut(s) 1773
LmnI GCTCC 3 cut(s) 1576, 2404, 3151
Lsp1109I GCAGC 4 cut(s) 182, 278, 528, 2921
LweI GCATC 7 cut(s) 475, 637, 1049, 1657, 1876, 1924, 2788
MabI ACCWGGT 2 cut(s) 222, 1480
MaeI CTAG 7 cut(s) 35, 447, 1157, 1251, 1508, 1847, 2870
MaeIII GTNAC 9 cut(s) 95, 403, 728, 820, 935, 1757, 1921, 2408, 2573
MflI RGATCY 4 cut(s) 210, 1180, 1385, 2010
MhlI GDGCHC 1 cut(s) 2023
MlsI TGGCCA 1 cut(s) 3025
MluNI TGGCCA 1 cut(s) 3025
MlyI GAGTC 2 cut(s) 352, 502
MmeI TCCRAC 4 cut(s) 198, 909, 2247, 2426
Mox20I TGGCCA 1 cut(s) 3025
Mph1103I ATGCAT 2 cut(s) 490, 963
MroXI GAANNNNTTC 3 cut(s) 859, 1269, 3071
MscI TGGCCA 1 cut(s) 3025
MseI TTAA 9 cut(s) 501, 1185, 1416, 1499, 1665, 2070, 2087, 2111, 3048
MslI CAYNNNNRTG 2 cut(s) 2220, 3098
Msp20I TGGCCA 1 cut(s) 3025
MspCI CTTAAG 1 cut(s) 1184
MspI CCGG 3 cut(s) 56, 1274, 2098
MspR9I CCNGG 7 cut(s) 178, 224, 605, 1057, 1482, 2099, 2150
Mva1269I GAATGC 4 cut(s) 139, 293, 328, 963
MvaI CCWGG 6 cut(s) 178, 224, 605, 1057, 1482, 2150
MvnI CGCG 2 cut(s) 147, 2368
MwoI GCNNNNNNNGC 2 cut(s) 300, 1794
NciI CCSGG 1 cut(s) 2099
NcoI CCATGG 1 cut(s) 3030
NdeI CATATG 1 cut(s) 2490
NlaIV GGNNCC 5 cut(s) 308, 1218, 1352, 2400, 2595
NmuCI GTSAC 5 cut(s) 820, 935, 1757, 1921, 2573
NsiI ATGCAT 2 cut(s) 490, 963
NspI RCATGY 7 cut(s) 258, 1403, 1965, 2120, 2608, 2642, 2947
PaeR7I CTCGAG 1 cut(s) 566
PagI TCATGA 2 cut(s) 1099, 2221
PasI CCCWGGG 1 cut(s) 604
PceI AGGCCT 1 cut(s) 3176
PciI ACATGT 3 cut(s) 254, 1961, 2638
PciSI GCTCTTC 1 cut(s) 1773
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PctI GAATGC 4 cut(s) 139, 293, 328, 963
PdmI GAANNNNTTC 3 cut(s) 859, 1269, 3071
PfeI GAWTC 7 cut(s) 82, 527, 860, 1062, 1265, 2207, 2370
Pfl23II CGTACG 1 cut(s) 238
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 4 cut(s) 172, 293, 543, 2911
PleI GAGTC 2 cut(s) 352, 501
PmaCI CACGTG 1 cut(s) 2563
PmlI CACGTG 1 cut(s) 2563
PpsI GAGTC 2 cut(s) 352, 501
Ppu21I YACGTR 1 cut(s) 2563
PpuMI RGGWCCY 2 cut(s) 1217, 1351
PscI ACATGT 3 cut(s) 254, 1961, 2638
PsiI TTATAA 2 cut(s) 1025, 1212
Psp1406I AACGTT 2 cut(s) 99, 1235
Psp5II RGGWCCY 2 cut(s) 1217, 1351
Psp6I CCWGG 6 cut(s) 176, 222, 603, 1055, 1480, 2148
PspCI CACGTG 1 cut(s) 2563
PspGI CCWGG 6 cut(s) 176, 222, 603, 1055, 1480, 2148
PspLI CGTACG 1 cut(s) 238
PspN4I GGNNCC 5 cut(s) 308, 1218, 1352, 2400, 2595
PspPI GGNCC 5 cut(s) 220, 1053, 1140, 1217, 1351
PspPPI RGGWCCY 2 cut(s) 1217, 1351
PstI CTGCAG 1 cut(s) 1587
PstNI CAGNNNCTG 1 cut(s) 2029
PsuI RGATCY 4 cut(s) 210, 1180, 1385, 2010
RseI CAYNNNNRTG 2 cut(s) 2220, 3098
SapI GCTCTTC 1 cut(s) 1773
SaqAI TTAA 9 cut(s) 501, 1185, 1416, 1499, 1665, 2070, 2087, 2111, 3048
SatI GCNGC 4 cut(s) 171, 292, 542, 2910
Sau96I GGNCC 5 cut(s) 220, 1053, 1140, 1217, 1351
ScaI AGTACT 1 cut(s) 1096
SchI GAGTC 2 cut(s) 352, 502
ScrFI CCNGG 7 cut(s) 178, 224, 605, 1057, 1482, 2099, 2150
SduI GDGCHC 1 cut(s) 2023
SexAI ACCWGGT 2 cut(s) 222, 1480
SfaNI GCATC 7 cut(s) 475, 637, 1049, 1657, 1876, 1924, 2788
SfcI CTRYAG 2 cut(s) 1583, 1980
Sfr274I CTCGAG 1 cut(s) 566
SinI GGWCC 5 cut(s) 220, 1053, 1140, 1217, 1351
SlaI CTCGAG 1 cut(s) 566
SmiMI CAYNNNNRTG 2 cut(s) 2220, 3098
SmlI CTYRAG 5 cut(s) 334, 566, 1184, 1768, 2467
SmoI CTYRAG 5 cut(s) 334, 566, 1184, 1768, 2467
SseBI AGGCCT 1 cut(s) 3176
SspI AATATT 3 cut(s) 1671, 2722, 3142
SspMI CTAG 7 cut(s) 35, 447, 1157, 1251, 1508, 1847, 2870
StuI AGGCCT 1 cut(s) 3176
StyD4I CCNGG 7 cut(s) 176, 222, 603, 1055, 1480, 2097, 2148
StyI CCWWGG 5 cut(s) 894, 1846, 2235, 2448, 3030
TaaI ACNGT 8 cut(s) 49, 69, 238, 935, 1921, 1931, 2265, 2715
TatI WGTACW 5 cut(s) 42, 1070, 1094, 1518, 2428
TfiI GAWTC 7 cut(s) 82, 527, 860, 1062, 1265, 2207, 2370
Tru1I TTAA 9 cut(s) 501, 1185, 1416, 1499, 1665, 2070, 2087, 2111, 3048
Tru9I TTAA 9 cut(s) 501, 1185, 1416, 1499, 1665, 2070, 2087, 2111, 3048
TscAI CASTG 6 cut(s) 940, 2010, 2064, 2380, 2387, 2888
TseFI GTSAC 5 cut(s) 820, 935, 1757, 1921, 2573
TseI GCWGC 4 cut(s) 170, 291, 541, 2909
Tsp45I GTSAC 5 cut(s) 820, 935, 1757, 1921, 2573
TspGWI ACGGA 4 cut(s) 1047, 1185, 1372, 2785
TspRI CASTG 6 cut(s) 940, 2010, 2064, 2380, 2387, 2888
Vha464I CTTAAG 1 cut(s) 1184
VneI GTGCAC 1 cut(s) 2019
VpaK11BI GGWCC 5 cut(s) 220, 1053, 1140, 1217, 1351
XagI CCTNNNNNAGG 2 cut(s) 337, 2736
XapI RAATTY 7 cut(s) 181, 455, 1412, 1444, 2768, 2788, 3071
XbaI TCTAGA 1 cut(s) 1250
XceI RCATGY 7 cut(s) 258, 1403, 1965, 2120, 2608, 2642, 2947
XhoI CTCGAG 1 cut(s) 566
XmaJI CCTAGG 1 cut(s) 1846
XmiI GTMKAC 2 cut(s) 1684, 1726
XmnI GAANNNNTTC 3 cut(s) 859, 1269, 3071
XspI CTAG 7 cut(s) 35, 447, 1157, 1251, 1508, 1847, 2870
ZraI GACGTC 1 cut(s) 2422
ZrmI AGTACT 1 cut(s) 1096
Zsp2I ATGCAT 2 cut(s) 490, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.