FvH4_2g36802

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000203
Physical Location & Seq
Forward (+)
26888047 .. 26893673
5627 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36802.t1

Sequence Viewer

Length: 3939 bp
ATGGCAGAGACAGTTGTTACAGAAGTTGTTGTGCCGGTTGCAAAATGGACATTGAAAAGAATTTGGCAAGAGTTGGGTTATGTGTTTAGTTACAAAAGCAACGTTGGGGATCTCAACAAGCAGGTTCAGGAACTGAGTGACAAAAGATATGGAGTGGACTTAGAGGTGAAACCTGCGAGAGAAGCTTTAAAGACTATTAATCCCGGAGTTGATCGCTGGCTCGGTGATGTAGACAAGATCATAAAAGAAAAGGAGACAAGTTTCAGTGAAGAAACTGTAGCAGCAAAGGCAGCATGCTGTAATGGGTGGCTTCCAAATTTGAAGTGTCGTTATTCTTTGGGAAAGAAAGCTAAGAATATGATTGAGAAGGTTGATGAGCTCTTTGCTAAAGATCTCGGGACAATAGCGCATCGTGCTCCTCACCCAGAAGTGGAATTCCAACCCACAGAGGACTCTATTCAGCTGGGAAAGAACTTCCATGAAGGTCCCAGCTCCTCAGGAACATCAAACCATGAACAGCCACCTGGCCTTCTTAATTTTGATTCAAGAAGATCAACCATTGGAGATGTGATGGAAGCTCTCAGGGGGAATCAAATCAATCCCATTATCATATGCGGCATGGGCGGCATTGGGAAGACAACATTGATGGGGCAGATTCTCGAGAAAGCAAAGGAAGAGGGTCTGTTCAATGAGTATACGAAGGCAACCCTCAAGGAATCTCTTAACAAAGCTGCTGACTTGTGTCTTATTCAAGATGAACTTGCTCGATATCTAGGCCTGTCACTTGACGGAAAGGAGACAGATGCAAGAGCAAGTATGCTACGTCAAAGACTATCTCAAGGCAGCAAGAAGATCCTCGTAATGTTAGACAATGTTTGGACAACAGGTCCAGACTTTCTGTGGGACATAGGGATTCCCCCTAGTTGTCAACTTTTGGTGACATCAAGACAACAGGATCTTTTCAAGAACATGGATACGAGAAAGAATTTCCCAATTCATGGTTTGTCGGATAGAGATGCCTGGAGTCTGTTCAAGAAGACAGCAGGGAGTTCCATCGAATCTGATCCCGAGTTGCATGTTGTAGCGGAAAAGGTTTTGAAGGAATGTGCTGGATTGCCGATTGCAATTTCAACTGTCGGAACGGCACTAAAAGGTGAAAGCATTGCAATTTGGAAGAATGCACTGAGGGAACTAGGAAAGGCTAGCCCAGAAAACGTTCCAGGAGTGGTAGAACATGTGTATGGTAAAATAAAGTTGAGCTATGAATGTTTGCCAAATGAGCAAGCCAAATCATTCTTGTTGCTGTGTTGCATATTTGAAGAAAGCGCTGATATACCGATTGAAGATTTGGTTAGATATGGGCATGGGCTTGGGCTCCTTAAAGGCATTGATTCAATGGTTGAAGGAAGGAACTGTTTGGAAACATTGGTTCACACACTCAAAAGTCGCTTTCTATTGTTAGATAGCGATCATAAAGAGTGTGTGAGAATGCATGATGTGGTACGTGATGTTGCCGTACATATAGCTTCTGAAGAGTTCGAGTTGGTTGAAAAGAAAAATGATGGGGTTGAAAAGAGAATTTTGGTGAGAGACAAAGCAAAAACTGCGCAGTTGATGGATTGGCTTAAGGATAGTGATTCTCATCAATGGAAATCTACTTATTGCAGTTCACTGGGTATCAGTGGGCCTTCTCAGCTTTTATTGTTTGGAAAGATACCTACATGTACAATTTCATTGGAGCTGCCACCCACAATTTTCAATAGAATGGAAGATCTCAGGGTTGTAGCAATTACTAATGGGGATAAATGGCATTGGAGTCATATGTCACTAGACTCACTACTGCCATCACTTGCCAATCTGAGGAACCTTCAAACACTGCGTCTAGAGACCTGTGATTTCCACTGTGGTGTATCCGTAATTGGGGAACTAAGGTCACTGATGATACTCAGTTTGCGTGGATGTTACATCAAGCAATTGCCTGATACATTTAAAAACTTGTCTGATTTAAGATTGCTTGATTTGACGGATTGCGAGTTTAATGTAATCTCACCTGGTGTGATATCAAGTTTAACCCGACTAGAAGAACTGTACATGTGGGGTAGCTTTGAGGACTGGGTGGTTGAGAAACTAGCCTCCACTGAAACAACCAATTGGATCTCTCAGTTGGAGGCTGAGCAGGAAAGTGCTGCACAGGTAGGAACGCAGAAGAAAAAAAGGAAAGTGGAGACGTGGAAGGGGTTCAACAGATTGGCAGTTGAGCAACCAGCAGAGGTAGTAATGGAGGAGTTTAGAGAATGGGTTCAATCTGGTACTTTAAAGAACACTGACTGTACAAGGTGGGCAAACTCTATGGACTGGGAAACTGGAATACTTGCAGAGTTACTTTCCTTGTCCCGTTTGACTACATTACAAGTTGTTCTGCCACCAGTCAATGTACTTCTAAGCAGCCCTCTCTTTAACAAGCTCGAAAGATTTAAGATCTTCATAGGATGGAAGCAGCATTTACGTAGATTTAAATTCAAGCATTTTGGGGTTGATGGCAACTACTTGAGAGTTCACGATCTTGATGCAAGCGCTACTGTGGGTAGTGGAATCACTTTGTTGCTAAAGAAAACCAGCTTTCTTGAGTTTAGTGCGAGTCAGACTCAACTTCTGAGTGTCTTGGATGCGCTCTGTTTTGCAAAGATCAAGCGTCTTAAATTCATGAATTCTAAACATGTGGAGTATTTGATTGACACGAAACTTGTGAATGACAGCAGCGCCATCTTTCCTGTTTTGAATTCATTGAAGATCAGATCTGCAAAGAGGCTGAAAGAGATTTTTCAAGGTGAGCTGCCTACGGGGTCTCTCAAGGAACTACGGAGTTTGACATTAAATAATCTACCTGCACTGACCTATATTTGGAAGACAAAGAGTCAATCTGATCAATGGGGTGTCTTGAGAAACATTTTAGGGCTCATTCAAGTGCTGAAAATAACACGCTGCAACTCCTTGGAAGCGATTGTTGCCTTTGAATATGATAATGAGGAAGTGATCAACTTCTCAAGTCTGACAGAGTTGACTCTCAATTATCTACCAAACTTCACTGGGATAAGCAAAAATATCTCTAAGCAGCCGGAAAGGCTACCAGAAGACAGCAGCTCGGAAGTATATTATGACGTTATCATACAACCTCTGTTTGACAGCAAGGTTCATTTCCCAGTCTTGTCAAAACTATACATTGACTGCGCAAACTTAAAAGAGATATGGAACAGCCAACTTTCAGCAGAATCCTTTTGTGAGCTAAGAATTCTACAGGTAGACGGATGTCATAAACTGTTGCATTTGGTACCATCTCATATGCAAAATAGATTGCAGAAATTGGAGGGCATACAAGCATATCGCTGTTCTTCACTTAAAGAGATTTTTGAATTTAGAAGATTGATTGTCAATGATGAAGGGGATGCTGCTGCTTTAACGATTTCCGAGTCAGGAAATCAAGGGATGCAAATCAACAAGCTGATGTCTTTCACACAATCTGGTCAAGCCTTTCAGAATCTTAGAGAGATATCAATTTATGATTGTAGAAGTTTGACAACTCTGCTCTCTCCCTCAATTGCCAGAGGTCTCGTGAAGCTTCAAGAACTAATGATAAAAAAGTGCGAGAAGATTGAAGAGATAGTAACACCAGCAGCAGAGGGTGAAGAATCAGAGGATGACAACATGTTCCCTCAGCTACATTCTTTGAGACTTGAGGATCTACCAAAGCTCAGAAGCTTTTCGCAAGGCAACTATAATTTCAAATGGCCACTAGCGGAAGACATAGAAATCCTCGAATGCAAAAATATGAAGAAGTTTTGTTCAGGATCCCTCAGCGCACCAAAGGAAGTGGAAATAAAGGTCGAAGGTGCTGGTGGGAGTGTTTTGCAAGAGCTCAATGACAGCAGACAAGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1313

Amino Acids

147.84

Weight (kDa)

5.99

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 186 - 349 2e-19 NB-ARC domain
LRR_14 PF23598 611 - 743 5.1e-09 Leucine-rich repeat region
LRR_8 PF13855 644 - 698 2.7e-07 Leucine rich repeat
LRR_RPS2 PF23247 889 - 973 1e-05 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1072 - 1215 4e-27 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1608, 3229
Acc36I ACCTGC 3 cut(s) 112, 181, 2893
Acc65I GGTACC 1 cut(s) 3328
AccB1I GGYRCC 1 cut(s) 3328
AccB7I CCANNNNNTGG 1 cut(s) 998
AccI GTMKAC 3 cut(s) 231, 695, 3300
AciI CCGC 4 cut(s) 615, 624, 1085, 3794
AclI AACGTT 2 cut(s) 102, 1215
AclWI GGATC 8 cut(s) 117, 847, 963, 1058, 2162, 3744, 3840, 3853
AcoI YGGCCR 1 cut(s) 3785
AcuI CTGAAG 1 cut(s) 1551
AdeI CACNNNGTG 1 cut(s) 2054
AfaI GTAC 8 cut(s) 1503, 1518, 1726, 2090, 2311, 2332, 2436, 3330
AfeI AGCGCT 2 cut(s) 1327, 2575
AfiI CCNNNNNNNGG 5 cut(s) 430, 998, 1860, 1920, 2901
AflII CTTAAG 1 cut(s) 1625
AflIII ACRYGT 5 cut(s) 1234, 1721, 2091, 2716, 3702
AhdI GACNNNNNGTC 2 cut(s) 885, 2913
AjiI CACGTC 1 cut(s) 2229
AjnI CCWGG 4 cut(s) 523, 1019, 1219, 2050
AleI CACNNNNGTG 1 cut(s) 1905
Alw21I GWGCWC 3 cut(s) 381, 418, 3915
Alw26I GTCTC 9 cut(s) 2, 248, 791, 1584, 1880, 2219, 2850, 3611, 3721
AlwI GGATC 8 cut(s) 117, 847, 963, 1058, 2162, 3744, 3840, 3853
AlwNI CAGNNNCTG 1 cut(s) 133
Ama87I CYCGRG 3 cut(s) 395, 659, 1067
Aor51HI AGCGCT 2 cut(s) 1327, 2575
AoxI GGCC 4 cut(s) 526, 775, 1685, 3785
ArsI GACNNNNNNTTYG 2 cut(s) 1864, 1896
AseI ATTAAT 1 cut(s) 198
Asp700I GAANNNNTTC 4 cut(s) 1215, 2237, 2298, 3757
Asp718I GGTACC 1 cut(s) 3328
AspLEI GCGC 8 cut(s) 409, 1328, 1609, 2576, 2671, 2762, 3230, 3857
AspS9I GGNCC 3 cut(s) 485, 887, 1685
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 9 cut(s) 178, 236, 413, 949, 1166, 1597, 2040, 2840, 3692
AsuNHI GCTAGC 1 cut(s) 1202
AvaI CYCGRG 3 cut(s) 395, 659, 1067
AvaII GGWCC 2 cut(s) 485, 887
AxyI CCTNAGG 1 cut(s) 496
BalI TGGCCA 1 cut(s) 3787
BamHI GGATCC 1 cut(s) 3845
BanI GGYRCC 1 cut(s) 3328
BanII GRGCYC 4 cut(s) 381, 1377, 2958, 3915
BauI CACGAG 1 cut(s) 3608
BbsI GAAGAC 5 cut(s) 641, 1043, 2912, 3138, 3804
Bbv12I GWGCWC 3 cut(s) 381, 418, 3915
BbvCI CCTCAGC 2 cut(s) 3711, 3851
BceAI ACGGC 2 cut(s) 1158, 1499
BcgI CGANNNNNNTGC 2 cut(s) 2549, 2583
BciT130I CCWGG 4 cut(s) 525, 1021, 1221, 2052
BciVI GTATCC 2 cut(s) 967, 1921
BclI TGATCA 2 cut(s) 2923, 3033
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 9 cut(s) 2, 248, 791, 1584, 1880, 2219, 2850, 3611, 3721
BfaI CTAG 9 cut(s) 773, 921, 1193, 1203, 1829, 1883, 2078, 2129, 3791
BfmI CTRYAG 2 cut(s) 276, 3293
BfoI RGCGCY 3 cut(s) 1329, 2577, 2763
BfrI CTTAAG 1 cut(s) 1625
BfuAI ACCTGC 3 cut(s) 112, 181, 2893
BfuI GTATCC 2 cut(s) 967, 1921
BglI GCCNNNNNGGC 1 cut(s) 3121
BglII AGATCT 4 cut(s) 391, 1771, 2478, 2795
BlpI GCTNAGC 1 cut(s) 2172
Bme1390I CCNGG 5 cut(s) 204, 525, 1021, 1221, 2052
Bme18I GGWCC 2 cut(s) 485, 887
BmeRI GACNNNNNGTC 2 cut(s) 885, 2913
BmeT110I CYCGRG 3 cut(s) 395, 659, 1067
BmgBI CACGTC 1 cut(s) 2229
BmgT120I GGNCC 3 cut(s) 485, 887, 1685
BmiI GGNNCC 5 cut(s) 487, 1376, 1865, 3330, 3847
BmrFI CCNGG 5 cut(s) 204, 525, 1021, 1221, 2052
BmrI ACTGGG 5 cut(s) 1682, 2122, 2365, 3096, 3194
BmsI GCATC 7 cut(s) 418, 793, 1006, 2557, 2656, 3433, 3474
BmtI GCTAGC 1 cut(s) 1206
BmuI ACTGGG 5 cut(s) 1682, 2122, 2365, 3096, 3194
BoxI GACNNNNGTC 2 cut(s) 741, 3306
BpiI GAAGAC 5 cut(s) 641, 1043, 2912, 3138, 3804
BplI GAGNNNNNCTC 2 cut(s) 2629, 2661
BpmI CTGGAG 1 cut(s) 1042
Bpu10I CCTNAGC 2 cut(s) 3711, 3851
Bpu1102I GCTNAGC 1 cut(s) 2172
BpuEI CTTGAG 8 cut(s) 695, 822, 2569, 2645, 2834, 2959, 3028, 3752
BpuMI CCSGG 1 cut(s) 204
BsaAI YACGTR 2 cut(s) 1505, 2507
BsaBI GATNNNNATC 3 cut(s) 1467, 1641, 3488
BsaI GGTCTC 3 cut(s) 1880, 2850, 3611
BsaJI CCNNGG 1 cut(s) 2991
Bsc4I CCNNNNNNNGG 5 cut(s) 430, 998, 1860, 1920, 2901
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 7 cut(s) 1677, 2117, 2360, 2368, 2426, 3091, 3200
Bse21I CCTNAGG 1 cut(s) 496
Bse3DI GCAATG 1 cut(s) 1161
Bse8I GATNNNNATC 3 cut(s) 1467, 1641, 3488
BseBI CCWGG 4 cut(s) 525, 1021, 1221, 2052
BseDI CCNNGG 1 cut(s) 2991
BseGI GGATG 7 cut(s) 1964, 2495, 2671, 3311, 3448, 3489, 3700
BseJI GATNNNNATC 3 cut(s) 1467, 1641, 3488
BseLI CCNNNNNNNGG 5 cut(s) 430, 998, 1860, 1920, 2901
BseMI GCAATG 1 cut(s) 1161
BseNI ACTGG 7 cut(s) 1677, 2117, 2360, 2368, 2426, 3091, 3200
BseRI GAGGAG 3 cut(s) 408, 484, 2297
BseYI CCCAGC 2 cut(s) 463, 488
BsgI GTGCAG 2 cut(s) 2172, 2871
BshFI GGCC 4 cut(s) 528, 777, 1687, 3787
BshNI GGYRCC 1 cut(s) 3328
BsiHKAI GWGCWC 3 cut(s) 381, 418, 3915
BsiHKCI CYCGRG 3 cut(s) 395, 659, 1067
BsiSI CCGG 3 cut(s) 35, 204, 3116
BslFI GGGAC 4 cut(s) 412, 471, 917, 2377
BslI CCNNNNNNNGG 5 cut(s) 430, 998, 1860, 1920, 2901
BsmAI GTCTC 9 cut(s) 2, 248, 791, 1584, 1880, 2219, 2850, 3611, 3721
BsmBI CGTCTC 1 cut(s) 2219
BsmFI GGGAC 4 cut(s) 412, 471, 917, 2377
BsmI GAATGC 3 cut(s) 1183, 1494, 3821
BsnI GGCC 4 cut(s) 528, 777, 1687, 3787
Bso31I GGTCTC 3 cut(s) 1880, 2850, 3611
BsoBI CYCGRG 3 cut(s) 395, 659, 1067
Bsp1286I GDGCHC 5 cut(s) 381, 418, 1377, 2958, 3915
Bsp1407I TGTACA 3 cut(s) 1724, 2088, 2330
Bsp1720I GCTNAGC 1 cut(s) 2172
BspACI CCGC 4 cut(s) 615, 624, 1085, 3794
BspANI GGCC 4 cut(s) 528, 777, 1687, 3787
BspHI TCATGA 1 cut(s) 2703
BspLI GGNNCC 5 cut(s) 487, 1376, 1865, 3330, 3847
BspMI ACCTGC 3 cut(s) 112, 181, 2893
BspOI GCTAGC 1 cut(s) 1206
BspPI GGATC 8 cut(s) 117, 847, 963, 1058, 2162, 3744, 3840, 3853
BspT107I GGYRCC 1 cut(s) 3328
BspTI CTTAAG 1 cut(s) 1625
BspTNI GGTCTC 3 cut(s) 1880, 2850, 3611
BsrDI GCAATG 1 cut(s) 1161
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 3 cut(s) 1724, 2088, 2330
BsrI ACTGG 7 cut(s) 1677, 2117, 2360, 2368, 2426, 3091, 3200
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 2991
BssNAI GTATAC 1 cut(s) 696
BssSI CACGAG 1 cut(s) 3608
BssT1I CCWWGG 1 cut(s) 2991
Bst1107I GTATAC 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 3608
Bst2UI CCWGG 4 cut(s) 525, 1021, 1221, 2052
Bst4CI ACNGT 9 cut(s) 13, 277, 1135, 1415, 1904, 2088, 2330, 2581, 3318
Bst6I CTCTTC 3 cut(s) 669, 1527, 3648
BstAFI CTTAAG 1 cut(s) 1625
BstAPI GCANNNNNTGC 1 cut(s) 1604
BstAUI TGTACA 3 cut(s) 1724, 2088, 2330
BstBAI YACGTR 2 cut(s) 1505, 2507
BstC8I GCNNGC 5 cut(s) 218, 295, 1204, 1284, 2572
BstF5I GGATG 7 cut(s) 1964, 2495, 2671, 3311, 3448, 3489, 3700
BstH2I RGCGCY 3 cut(s) 1329, 2577, 2763
BstHHI GCGC 8 cut(s) 409, 1328, 1609, 2576, 2671, 2762, 3230, 3857
BstMAI GTCTC 9 cut(s) 2, 248, 791, 1584, 1880, 2219, 2850, 3611, 3721
BstNI CCWGG 4 cut(s) 525, 1021, 1221, 2052
BstNSI RCATGY 7 cut(s) 297, 1079, 1238, 1725, 2095, 2720, 3706
BstPAI GACNNNNGTC 2 cut(s) 741, 3306
BstSCI CCNGG 5 cut(s) 202, 523, 1019, 1219, 2050
BstSFI CTRYAG 2 cut(s) 276, 3293
BstSNI TACGTA 1 cut(s) 2507
BstV2I GAAGAC 5 cut(s) 641, 1043, 2912, 3138, 3804
BstZ17I GTATAC 1 cut(s) 696
Bsu36I CCTNAGG 1 cut(s) 496
BsuI GTATCC 2 cut(s) 967, 1921
BsuRI GGCC 4 cut(s) 528, 777, 1687, 3787
BtrI CACGTC 1 cut(s) 2229
BtsCI GGATG 7 cut(s) 1964, 2495, 2671, 3311, 3448, 3489, 3700
BtsI GCAGTG 1 cut(s) 1874
BveI ACCTGC 3 cut(s) 112, 181, 2893
Cac8I GCNNGC 5 cut(s) 218, 295, 1204, 1284, 2572
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 1 cut(s) 2703
CfoI GCGC 8 cut(s) 409, 1328, 1609, 2576, 2671, 2762, 3230, 3857
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 485, 887, 1685
CseI GACGC 2 cut(s) 1868, 2681
CsiI ACCWGGT 1 cut(s) 2050
Csp6I GTAC 8 cut(s) 1502, 1517, 1725, 2089, 2310, 2331, 2435, 3329
CspCI CAANNNNNGTGG 2 cut(s) 3849, 3884
CviQI GTAC 8 cut(s) 1502, 1517, 1725, 2089, 2310, 2331, 2435, 3329
DraI TTTAAA 4 cut(s) 189, 1990, 2316, 2515
DraIII CACNNNGTG 1 cut(s) 2054
DriI GACNNNNNGTC 2 cut(s) 885, 2913
EaeI YGGCCR 1 cut(s) 3785
Eam1104I CTCTTC 3 cut(s) 669, 1527, 3648
Eam1105I GACNNNNNGTC 2 cut(s) 885, 2913
EarI CTCTTC 3 cut(s) 669, 1527, 3648
Ecl136II GAGCTC 2 cut(s) 379, 3913
Eco105I TACGTA 1 cut(s) 2507
Eco130I CCWWGG 1 cut(s) 2991
Eco147I AGGCCT 1 cut(s) 777
Eco24I GRGCYC 4 cut(s) 381, 1377, 2958, 3915
Eco31I GGTCTC 3 cut(s) 1880, 2850, 3611
Eco32I GATATC 3 cut(s) 770, 2061, 3549
Eco47I GGWCC 2 cut(s) 485, 887
Eco47III AGCGCT 2 cut(s) 1327, 2575
Eco53kI GAGCTC 2 cut(s) 379, 3913
Eco57I CTGAAG 1 cut(s) 1551
Eco81I CCTNAGG 1 cut(s) 496
Eco88I CYCGRG 3 cut(s) 395, 659, 1067
EcoICRI GAGCTC 2 cut(s) 379, 3913
EcoO109I RGGNCCY 1 cut(s) 485
EcoRI GAATTC 4 cut(s) 434, 2707, 2779, 3288
EcoRII CCWGG 4 cut(s) 523, 1019, 1219, 2050
EcoRV GATATC 3 cut(s) 770, 2061, 3549
EcoT14I CCWWGG 1 cut(s) 2991
EcoT22I ATGCAT 1 cut(s) 1494
EcoT38I GRGCYC 4 cut(s) 381, 1377, 2958, 3915
ErhI CCWWGG 1 cut(s) 2991
Esp3I CGTCTC 1 cut(s) 2219
FalI AAGNNNNNCTT 2 cut(s) 744, 776
FaqI GGGAC 4 cut(s) 412, 471, 917, 2377
FauNDI CATATG 3 cut(s) 611, 1821, 3339
FbaI TGATCA 2 cut(s) 2923, 3033
FblI GTMKAC 3 cut(s) 231, 695, 3300
FokI GGATG 7 cut(s) 1971, 2502, 2678, 3318, 3455, 3496, 3707
FriOI GRGCYC 4 cut(s) 381, 1377, 2958, 3915
FspBI CTAG 9 cut(s) 773, 921, 1193, 1203, 1829, 1883, 2078, 2129, 3791
FspI TGCGCA 2 cut(s) 1608, 3229
GlaI GCGC 8 cut(s) 408, 1327, 1608, 2575, 2670, 2761, 3229, 3856
GsaI CCCAGC 2 cut(s) 467, 492
GsuI CTGGAG 1 cut(s) 1042
HaeII RGCGCY 3 cut(s) 1329, 2577, 2763
HaeIII GGCC 4 cut(s) 528, 777, 1687, 3787
HapII CCGG 3 cut(s) 35, 204, 3116
HgaI GACGC 2 cut(s) 1868, 2681
HhaI GCGC 8 cut(s) 409, 1328, 1609, 2576, 2671, 2762, 3230, 3857
Hin6I GCGC 8 cut(s) 407, 1326, 1607, 2574, 2669, 2760, 3228, 3855
HinP1I GCGC 8 cut(s) 407, 1326, 1607, 2574, 2669, 2760, 3228, 3855
HincII GTYRAC 2 cut(s) 929, 3060
HindII GTYRAC 2 cut(s) 929, 3060
HindIII AAGCTT 3 cut(s) 183, 3614, 3754
HpaII CCGG 3 cut(s) 35, 204, 3116
HphI GGTGA 9 cut(s) 178, 236, 413, 949, 1166, 1597, 2040, 2840, 3692
Hpy166II GTNNAC 9 cut(s) 157, 232, 696, 929, 1432, 1670, 2557, 3060, 3301
Hpy8I GTNNAC 9 cut(s) 157, 232, 696, 929, 1432, 1670, 2557, 3060, 3301
HpyCH4III ACNGT 9 cut(s) 13, 277, 1135, 1415, 1904, 2088, 2330, 2581, 3318
HpyCH4IV ACGT 7 cut(s) 102, 823, 1215, 1504, 2228, 2506, 3159
HpySE526I ACGT 7 cut(s) 102, 823, 1215, 1504, 2228, 2506, 3159
HspAI GCGC 8 cut(s) 407, 1326, 1607, 2574, 2669, 2760, 3228, 3855
KpnI GGTACC 1 cut(s) 3332
Ksp22I TGATCA 2 cut(s) 2923, 3033
LmnI GCTCC 4 cut(s) 421, 497, 1380, 1738
LweI GCATC 7 cut(s) 418, 793, 1006, 2557, 2656, 3433, 3474
MabI ACCWGGT 1 cut(s) 2050
MaeI CTAG 9 cut(s) 773, 921, 1193, 1203, 1829, 1883, 2078, 2129, 3791
MaeII ACGT 7 cut(s) 102, 823, 1215, 1504, 2228, 2506, 3159
MfeI CAATTG 3 cut(s) 1973, 2149, 3594
MhlI GDGCHC 5 cut(s) 381, 418, 1377, 2958, 3915
MlsI TGGCCA 1 cut(s) 3787
MluNI TGGCCA 1 cut(s) 3787
MlyI GAGTC 9 cut(s) 446, 1033, 1825, 1826, 2638, 2647, 2923, 3055, 3476
MmeI TCCRAC 4 cut(s) 463, 987, 1117, 2145
Mox20I TGGCCA 1 cut(s) 3787
Mph1103I ATGCAT 1 cut(s) 1494
MroXI GAANNNNTTC 4 cut(s) 1215, 2237, 2298, 3757
MscI TGGCCA 1 cut(s) 3787
MslI CAYNNNNRTG 3 cut(s) 1239, 1905, 2963
Msp20I TGGCCA 1 cut(s) 3787
MspA1I CMGCKG 1 cut(s) 463
MspCI CTTAAG 1 cut(s) 1625
MspI CCGG 3 cut(s) 35, 204, 3116
MspR9I CCNGG 5 cut(s) 204, 525, 1021, 1221, 2052
MunI CAATTG 3 cut(s) 1973, 2149, 3594
Mva1269I GAATGC 3 cut(s) 1183, 1494, 3821
MvaI CCWGG 4 cut(s) 525, 1021, 1221, 2052
NciI CCSGG 1 cut(s) 204
NdeI CATATG 3 cut(s) 611, 1821, 3339
NheI GCTAGC 1 cut(s) 1202
NlaIV GGNNCC 5 cut(s) 487, 1376, 1865, 3330, 3847
NmuCI GTSAC 5 cut(s) 137, 780, 937, 1824, 1932
NsbI TGCGCA 2 cut(s) 1608, 3229
NsiI ATGCAT 1 cut(s) 1494
NspI RCATGY 7 cut(s) 297, 1079, 1238, 1725, 2095, 2720, 3706
OliI CACNNNNGTG 1 cut(s) 1905
PaeI GCATGC 1 cut(s) 297
PaeR7I CTCGAG 1 cut(s) 659
PagI TCATGA 1 cut(s) 2703
PceI AGGCCT 1 cut(s) 777
PciI ACATGT 5 cut(s) 1234, 1721, 2091, 2716, 3702
PctI GAATGC 3 cut(s) 1183, 1494, 3821
PdmI GAANNNNTTC 4 cut(s) 1215, 2237, 2298, 3757
PflMI CCANNNNNTGG 1 cut(s) 998
PfoI TCCNGGA 2 cut(s) 202, 1219
PleI GAGTC 9 cut(s) 446, 1032, 1824, 1826, 2638, 2646, 2922, 3055, 3475
PpsI GAGTC 9 cut(s) 446, 1032, 1824, 1826, 2638, 2646, 2922, 3055, 3475
Ppu21I YACGTR 2 cut(s) 1505, 2507
PpuMI RGGWCCY 1 cut(s) 485
PscI ACATGT 5 cut(s) 1234, 1721, 2091, 2716, 3702
PshAI GACNNNNGTC 2 cut(s) 741, 3306
PshBI ATTAAT 1 cut(s) 198
Psp124BI GAGCTC 2 cut(s) 381, 3915
Psp1406I AACGTT 2 cut(s) 102, 1215
Psp5II RGGWCCY 1 cut(s) 485
Psp6I CCWGG 4 cut(s) 523, 1019, 1219, 2050
PspFI CCCAGC 2 cut(s) 463, 488
PspGI CCWGG 4 cut(s) 523, 1019, 1219, 2050
PspN4I GGNNCC 5 cut(s) 487, 1376, 1865, 3330, 3847
PspPI GGNCC 3 cut(s) 485, 887, 1685
PspPPI RGGWCCY 1 cut(s) 485
PstNI CAGNNNCTG 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 8 cut(s) 1503, 1518, 1726, 2090, 2311, 2332, 2436, 3330
RsaNI GTAC 8 cut(s) 1502, 1517, 1725, 2089, 2310, 2331, 2435, 3329
RseI CAYNNNNRTG 3 cut(s) 1239, 1905, 2963
SacI GAGCTC 2 cut(s) 381, 3915
Sau96I GGNCC 3 cut(s) 485, 887, 1685
SchI GAGTC 9 cut(s) 446, 1033, 1825, 1826, 2638, 2647, 2923, 3055, 3476
ScrFI CCNGG 5 cut(s) 204, 525, 1021, 1221, 2052
SduI GDGCHC 5 cut(s) 381, 418, 1377, 2958, 3915
SexAI ACCWGGT 1 cut(s) 2050
SfaNI GCATC 7 cut(s) 418, 793, 1006, 2557, 2656, 3433, 3474
SfcI CTRYAG 2 cut(s) 276, 3293
Sfr274I CTCGAG 1 cut(s) 659
SinI GGWCC 2 cut(s) 485, 887
SlaI CTCGAG 1 cut(s) 659
SmiI ATTTAAAT 1 cut(s) 2515
SmiMI CAYNNNNRTG 3 cut(s) 1239, 1905, 2963
SnaBI TACGTA 1 cut(s) 2507
SphI GCATGC 1 cut(s) 297
SseBI AGGCCT 1 cut(s) 777
SsiI CCGC 4 cut(s) 615, 624, 1085, 3794
SspMI CTAG 9 cut(s) 773, 921, 1193, 1203, 1829, 1883, 2078, 2129, 3791
SstI GAGCTC 2 cut(s) 381, 3915
StuI AGGCCT 1 cut(s) 777
StyD4I CCNGG 5 cut(s) 202, 523, 1019, 1219, 2050
StyI CCWWGG 1 cut(s) 2991
SwaI ATTTAAAT 1 cut(s) 2515
TaaI ACNGT 9 cut(s) 13, 277, 1135, 1415, 1904, 2088, 2330, 2581, 3318
TaiI ACGT 7 cut(s) 105, 826, 1218, 1507, 2231, 2509, 3162
TaqI TCGA 7 cut(s) 660, 766, 1056, 1539, 2466, 3813, 3882
TatI WGTACW 4 cut(s) 1724, 2088, 2330, 2434
TauI GCSGC 2 cut(s) 618, 627
TseFI GTSAC 5 cut(s) 137, 780, 937, 1824, 1932
Tsp45I GTSAC 5 cut(s) 137, 780, 937, 1824, 1932
TspGWI ACGGA 5 cut(s) 804, 1903, 2039, 2875, 3318
Van91I CCANNNNNTGG 1 cut(s) 998
Vha464I CTTAAG 1 cut(s) 1625
VpaK11BI GGWCC 2 cut(s) 485, 887
VspI ATTAAT 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 1882
XceI RCATGY 7 cut(s) 297, 1079, 1238, 1725, 2095, 2720, 3706
XcmI CCANNNNNNNNNTGG 1 cut(s) 897
XhoI CTCGAG 1 cut(s) 659
XmiI GTMKAC 3 cut(s) 231, 695, 3300
XmnI GAANNNNTTC 4 cut(s) 1215, 2237, 2298, 3757
XspI CTAG 9 cut(s) 773, 921, 1193, 1203, 1829, 1883, 2078, 2129, 3791
Zsp2I ATGCAT 1 cut(s) 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.