Rh6CG260100

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000203
Physical Location & Seq
Reverse (-)
43252660 .. 43259258
6599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG260100.1

Sequence Viewer

Length: 4851 bp
ATGGCAGATATTGTGATTACAGTTGTTGGAAAATTAGCAGAGTACACAGTCGCACCTGTTATGCGACAGTTTGGTTATCTGATTAACTACAAGAGCAATGTTAAAGATCTCAGAGAGAAGGTTACAAGTCTGACTGCCAAAAGAGAGGGGGTACTACAACGTGTGGATACAGCAAACAGGAACTTGGAGGCAATTCTTCCCGAAGTTCAGAACTGGTTGGAGCGAGTAGACCAGACCATAAAAGAAAAGGAGACATGTTTTGAAGAAGGAAGAGTAGCCAAGGCAACAACATGCTGCAGCTCCAATGGGTGGTGTCCAAATCTGAAGGTCCGTCATTCCTTGAGTAGGACAGCAAAGAAGATGGCTCCAAACGTTGATCACCTTATTACAGATGGAAATTTTACCACAATAACTTGTCGTGCTCCTCCGCGGAAGATTGATTATCCAGCCATTCAATATCCTGGGGATTCCACTGAGGCACCAGCGAAGGGGGCTAGCTCCTCCTCAGGAACATCCGATCCTGCTAAGCCACCTGCACCTATGGATCTCTCTGAGCGTCTGAAATATAGGTTGCCTTATACAAAGGATCTCTTGACGGCTCTGCAAGATGACAAGATCAGCATGATCGGCATTTCTGGGACAATCAGAGTGATAGATACAGTAACAACTAAAGAGTTCATGAAAAGAATGAAACAGAAAAAGCTCTTTGAAGAGGTTGCGATGGCAGTTGTGTCCCAGGATCCAGACTTGGAACGAATACAAGCCGTGTATATAGCCTCCAAAGAGTTAGCACAAAAGAAGAGTGATAAGCTTGAAAGGAGATATATGGAAAGAGGAGGGGTGGAATTGAACGATTGGCTCAAGAGCAGTTCACTGAGAGCCGATATGAGTACTACTGAGCTTTTAGTGTTGGGACCCACAGATGGAATTGAACTGGATCTGCCAGCCACAATTTTCGATGGAATGGAAAATCTCAAGGTTTTAATGATCTGGTGTTCAATCCTCCCATCCCTTTCACTTCTGAAGAATCTTCAAACACTCAGTCTAGGGTGTTGCCGTCGCAATATTGATGTCATTGGAGAGCTACGGTCACTCATGATACTCAGGTTATATGGATCTGGCATCAAACAGTTGCCTGATACATTCAAAAATTTATCTAATCTGAGGTTGTTAAACTTGACAGGATGTACAGAGCTTAAAGTAATCTCACCAGGTGTTATATCGAGTTTATCTCGTCTAGAAGAATTGTACATGCCGAATAACTTTCATGGTTGGTTGGTTGAGAAACTGGGCTCAACAGAAACAATCAATTGGATCTCTCAATTGAAGGTTGATGCCCAACCAGAAGAAGTTGACAGATGGAAGAAATTCAACAGGTTGGCGGTTGAGAGGCTAGCCTCAGCAGAAACAACGACTGATCAGGTCTCTCAACTGGAGGATGAGGAAAGTAGCGAATCAGAGGAACTCGACTTCCTTGGATATCTGTTCGCAGAGTATGTTCGAGATTTAAAAGGAGACTACGACGATCTCACAAGGTGGGCCAAATCTATGGACTGGAGAGTTGGATTACTTTCAGAGCTACTTTCCTTGTCCCGTTTGACTACTTTAGAAGTGGTTTTACCACCAATTGAGATTCTGCGAACCAGTAAGCTATTTCATAAGCTTGAAAGATTTAAGATCTCTATTGGATGGGATGAGCATGAAGACTTGGAGGACTTAGAGGGTAACTACTTGAGAGTTTATAGTCTTGATGGAAGCTCTCTTGCGGATACTGAAATCAGTTTGTTGTTGAGGAAAACTAACCAGCTCGAGTTAAAAATGAAGAATTTGAGAGACCCTCTCAATGTCTTAGATGTAAACTGCTGTGCAAACTTGGAGTCACTTGCCCTCAAAGACTGTGATTCTCTGGACTATTTGATTGACATGACTCTTAAGCAAGATACTCCACGAAGCGTGTTTCCTGTCTTGAATTATCTGGAAATCAATGGAGCAAGGAGGCTGAAAGAGATTTTTCATGGTGACCTGCCACTTGGATCGCTTCAGAAACTAGAAAGGCTAACTTTACGGGATTGCAAGTCATTGGAAGCAATTTTTGCCTTGGAAGGATCGGAACATCATGTCAAAGAGATCAACTTCCCTAGTTTGACAAAGTTGGAACTTAAAGGTCTCCCGAGCTTCACTTTCCAGTCATTGGATCTAAACTGCTGTGCAAACTTGGAGTCACTTACCCTCAAAGACTATGATTCTCTGGAATATCTGATTGACATGACTCTTAAGCAAGATACTCCACGAAGCGTCTTTCCTGTCTTGAATTATCTGGAAATCAATAGAGCAAGGAGGTTGAAAGAGATTTTTCATGGTGAGATGCCACTGGGATCGCTTCAGAAACTAGAAAGGCTAACTTTATGGGATTGCAAGTCATTGGAAGCAATTTTTGCCATGGAAGGATCGGGACATCATGTCAAAGAGATCAACTTCCTTTGTTTGACAAAGTTGGAACTTAAAGGTCTCCCGAGCTTCACTTTCCAGTCATTGGATCTAAACTGCTGTGCAAACTTGAAGTCACTTACCCTCAAAGACTGTGATTCTCTGGAATATCTGATTGACATGACTCTTAAGCAAGATACTCCACGAAGCGTCTTTCCTGTCTTGAATTATCTGGAAATCAATAGAGCAAGGAGGATGAAAGAGATTTTTCATGGTGAGATGCCACTGGGATCGCTTCAGAAACTAGAAAGGCTAACTTTAGGGGATTGCAAGTCATTGGAAGCGATTTTTGCCATGGAAGGATCGGGACATCATGTCAAAGAGATCAACTTCCTTTGTTTGACAAAGTTGGAACTTAAAGGTCTCCCGAGCTTCACTTTCCAGTCATTGGATCTAAACTGCTGTGCAAACTTGAAGTCACTTACCCTCAAAGACTGTGATTCTCTGGAATATCTGATTGACATGACTCTTAAGCAAGATACTCCACGAAGCGTCTTTCCTGTCTTGAATTATCTGGAAATCAATGGAGCAAGGAGGCTGAAAGAGATTTTTCATGGTGAGTTGCCACTGGGATCGCTTCAGAAACTAGAAAGGCTAACTTTACGGGATTGCCAGTCATTGGAAGCAATTTTTGCCTTGGAAGGATCAGGACATCATGTCAAAGAGATCAACTTCCTTTGTTTGACAAAGTTGGAACTTAAAGGTCTCCCGAGCTTCACTTGCCAGTCATTGGATCTAAACTGCTGTGCAAACTTGGAGTCACTTACCCTCAAAAACTGTGATTCTCTGGAATATCTGATTGACATGACTCTTAAGCAAGATAATCCACGAAGCGTCTTTCCTGTCTTGAATTATCTGGAAATCAATAGAGCAAGGAGGTTGAAAGAGATTTTTCATGGTGATCTGCCACTGGGATCGCTTCAGAAACTAGAAAGGCTAACTTTACGGGATTGCCAGTCATTGGAAGAAATTTTTGCCTTGGAAGGATCGGGACATCATGTCAAAGAGATCAACTTCCTTTGTTTGACAAAGTTGGAACTTAAAGGTCTCCCGAGCTTCACTTTCCAGTCATTGGATCTAAACTGCTGTGCAAACTTGGAGTCACTTACCCTCGAAGACTGTGATTCTCTGGAATGTCTGATTGACCAAACTCTTAAGCAAGATACTCCACGAAGCGTCTTTCCTGTCTTGAATTATCTGGAAATCAATGGAGCAAGGAGGCTGAAAGAGATTTTTCATGGTGACCTGCCACTGGGGTCGCTTCAGAAACTAGAAAGGCTATTGTTACAGAATCTACCTGCATTGACGACTATTTGGACTATTGGAAGTCAATCTAGATGGTTGGGAGATCTGGCCGCTGTCTATGACATATCAATATGGGATTGCCAGTCATTGGAAGCCATTTTTGCGTTGGAAGGATTGGGACATCATGTCAAAGAGATCAACTTCCTTAGTTTGAGGCGGTTGAAACTTAAAAGTCTACCAAGCTTCACTGGAATGAGCAAAAGTACTTGTAAGGGGATGGAAAGGCAACTGGAAGTCAGCAGTCTCCATCACAATAGTTTGACCGTTGAACATTCACTTTTCTCTGACCCCAAGGTTGTCTTTCCCGTCTTGGAATCCCTGAACATTAGCCGTCTGGGCAACTTGAAAGAGATATGGAATAGCCGACTTTCACCCAACTCCTTCTCTGAATTACGAACGCTCACTGTCGAGGATTGTGACAAACTATTACATTTGGGACCAACACAAATGCAAAATAGATTGCAGAGATTGCAGTACATAAAGGTAAGGAACTGTTCTTCACTGGAAGAGATTTTTGAAGTCGGAAGATTGACCGTCAATGAAGGGAATGCTTCAATTTCTCAGTCAGACAAAATACCTTCAAATATTTCCCAATCTGATCAAGGGATGCAAATAAATGATATCATGGATTTCAAACAATCTTGTCAAGGCTTTCAGAATTTAACCGTACTAGTAGTTAGATCTTGTGGCAGTTTGAGATACCTGCTCTCTCCTTCGATTGCCAGAGGTCTTGTCAAGCTCGGATATCTAGATATAAGATCTTGCGAGAATATCGAAGCAATAGTCGCAGCAGATGAGGGTGAAGAAACAGAGAATGAAAGCATGCTCCCTCAGCTATATTATTTGCATCTTGGGGATCTACCCAATCTTGGAAGCTTTTCGCAAGGCAAATATACTTTTGATTGGCCACGAGTGAGACGGATCAAAATTAAAAAATGCAACAAAATGAACAACTTTTGTTCAGGATCCCTCAGCATCCGAAGGGAAGTGAAGATAATTGTCCGTGATTCTGGTGAGAATCTTGAGCAGGAGCTGAATGATAGCAAGAAAGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1616

Amino Acids

183.22

Weight (kDa)

5.71

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 332 - 478 9.8e-06 Leucine-rich repeat region
LRR_8 PF13855 363 - 421 3.1e-07 Leucine rich repeat
LRR_RPS2 PF23247 648 - 759 2.4e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 762 - 873 4.3e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 876 - 987 3.6e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 990 - 1100 6.6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1105 - 1212 9e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1189 - 1299 9.3e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1369 - 1520 4.8e-26 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1472 - 1584 3.5e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000203)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02740 FvH4_1g02750 FvH4_2g17380 FvH4_2g17381 FvH4_2g17400 FvH4_2g17410 FvH4_2g17420 FvH4_2g36800 FvH4_2g36802 FvH4_2g36810 FvH4_2g36830 FvH4_2g36840 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36850 FvH4_2g36860 FvH4_4g35200 FvH4_4g35200 FvH4_4g35200 FvH4_6g02712 FvH4_6g02712 FvH4_6g02713 FvH4_6g02760 FvH4_6g02790 FvH4_6g02820
prunus_persica Prupe.8G185600_v2.0.a1 Prupe.8G185700_v2.0.a1 Prupe.8G185800_v2.0.a1
pyrus_communis pycom05g14750
rosa_chinensis RchiOBHm_Chr1g0341391 RchiOBHm_Chr5g0061651 RchiOBHm_Chr5g0061661 RchiOBHm_Chr5g0080761 RchiOBHm_Chr6g0281031 RchiOBHm_Chr6g0282051 RchiOBHm_Chr6g0304941
rosa_laevigata RLG00000003533 RLG00000005089 RLG00000008660 RLG00000010934 RLG00000010935 RLG00000010936 RLG00000010937 RLG00000012912 RLG00000012917 RLG00000013003 RLG00000013004 RLG00000035489 RLG00000035491 RLG00000035492
rosa_multiflora Rmu_co8167864.1_g000001 Rmu_sc0000014.1_g000008 Rmu_sc0000014.1_g000011 Rmu_sc0000014.1_g000012 Rmu_sc0000047.1_g000008 Rmu_sc0000301.1_g000009 Rmu_sc0000569.1_g000011 Rmu_sc0000569.1_g000020 Rmu_sc0001700.1_g000009 Rmu_sc0001700.1_g000016 Rmu_sc0001700.1_g000032 Rmu_sc0001700.1_g000044 Rmu_sc0002720.1_g000017 Rmu_sc0005149.1_g000009 Rmu_sc0005210.1_g000001 Rmu_sc0009256.1_g000004 Rmu_sc0012869.1_g000004 Rmu_sc0016179.1_g000008 Rmu_sc0016179.1_g000009 Rmu_sc0018903.1_g000001 Rmu_sc0018903.1_g000003 Rmu_sc0024112.1_g000001 Rmu_sc0030824.1_g000001 Rmu_sc0036328.1_g000001 Rmu_sc0041575.1_g000001 Rmu_ssc0000091.1_g000011 Rmu_ssc0000173.1_g000025
rosa_roxburghii Rroxscaffold_1G00019090 Rroxscaffold_3G00270850 Rroxscaffold_3G00270870 Rroxscaffold_4G00312030 Rroxscaffold_7G00163330 Rroxscaffold_7G00186580 Rroxscaffold_7G00187370
rosa_rugosa Rorug01G0156700.1 Rorug01G0156800.1 Rorug01G0156900.1 Rorug03G0141600 Rorug03G0141700 Rorug03G0141800 Rorug04G0084700 Rorug04G0084700 Rorug05G0298800 Rorug06G0145000 Rorug06G0145200 Rorug06G0336400 Rorug06G0336400 Rorug06G0451600 Rorug06G0451800
rosa_samantha Rh1AG171900 Rh1BG139600 Rh1CG159700 Rh1CG159800 Rh1CG159900 Rh1DG172000 Rh4AG149300 Rh4BG146200 Rh4CG156500 Rh4DG142300 Rh5AG403900 Rh5BG416500 Rh5CG440700 Rh5CG440800 Rh5CG441000 Rh5CG441100 Rh5DG430500 Rh5DG430700 Rh6AG250300 Rh6AG258200 Rh6AG447600 Rh6BG252600 Rh6BG261200 Rh6BG261700 Rh6BG453400 Rh6BG453500 Rh6BG453700 Rh6CG253000 Rh6CG260100 Rh6CG260500 Rh6CG461200 Rh6DG244700 Rh6DG252500 Rh6DG448200 Rh7AG055000 Rh7AG055100 Rh7AG055200 Rh7BG054500 Rh7BG054600 Rh7CG055900 Rh7DG054600 Rh7DG054700
rosa_wichuraiana Rw0G009830 Rw0G014970 Rw1G014320 Rw5G037960 Rw6G021650 Rw6G021720 Rw6G022330 Rw6G022350 Rw6G038980 Rw7G004490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 541
Acc36I ACCTGC 5 cut(s) 541, 2031, 3741, 3793, 4503
AccB1I GGYRCC 1 cut(s) 478
AccB7I CCANNNNNTGG 9 cut(s) 309, 2191, 2533, 2875, 3106, 3217, 3448, 3559, 3880
AccI GTMKAC 2 cut(s) 228, 3967
AccII CGCG 1 cut(s) 430
AciI CCGC 6 cut(s) 428, 430, 1382, 1766, 3843, 3949
AclI AACGTT 1 cut(s) 372
AcoI YGGCCR 2 cut(s) 3840, 4697
AcsI RAATTY 6 cut(s) 397, 1150, 1367, 1825, 3456, 4450
AcuI CTGAAG 8 cut(s) 344, 1043, 2024, 2366, 2708, 3050, 3392, 3734
AdeI CACNNNGTG 2 cut(s) 1214, 1536
AfaI GTAC 8 cut(s) 44, 153, 892, 1189, 1250, 3997, 4268, 4461
AflII CTTAAG 6 cut(s) 1931, 2273, 2615, 2957, 3299, 3641
AflIII ACRYGT 2 cut(s) 160, 254
AhdI GACNNNNNGTC 5 cut(s) 2459, 2801, 3143, 3485, 3917
AhlI ACTAGT 1 cut(s) 4462
AjnI CCWGG 3 cut(s) 460, 735, 1210
AloI GAACNNNNNNTCC 4 cut(s) 502, 534, 1455, 1487
Alw21I GWGCWC 1 cut(s) 424
AlwNI CAGNNNCTG 1 cut(s) 4825
Ama87I CYCGRG 6 cut(s) 1808, 2170, 2512, 2854, 3196, 3538
AoxI GGCC 3 cut(s) 1539, 3840, 4697
ApeKI GCWGC 3 cut(s) 294, 297, 4580
ApoI RAATTY 6 cut(s) 397, 1150, 1367, 1825, 3456, 4450
Asp700I GAANNNNTTC 2 cut(s) 1367, 4669
AspS9I GGNCC 4 cut(s) 328, 914, 1539, 4229
AsuNHI GCTAGC 2 cut(s) 494, 1393
AvaI CYCGRG 6 cut(s) 1808, 2170, 2512, 2854, 3196, 3538
AvaII GGWCC 3 cut(s) 328, 914, 4229
AxyI CCTNAGG 1 cut(s) 505
BaeI ACNNNNGTAYC 2 cut(s) 1091, 1124
BalI TGGCCA 1 cut(s) 4699
BamHI GGATCC 2 cut(s) 739, 4757
BanI GGYRCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 1295
BarI GAAGNNNNNNTAC 2 cut(s) 1602, 1634
BauI CACGAG 1 cut(s) 4701
BbsI GAAGAC 2 cut(s) 1710, 3609
Bbv12I GWGCWC 1 cut(s) 424
BbvCI CCTCAGC 3 cut(s) 1399, 4623, 4763
BbvI GCAGC 3 cut(s) 281, 309, 4592
BceAI ACGGC 4 cut(s) 612, 749, 1041, 4107
BciT130I CCWGG 3 cut(s) 462, 737, 1212
BciVI GTATCC 2 cut(s) 160, 1762
BclI TGATCA 3 cut(s) 376, 1417, 4390
BcuI ACTAGT 1 cut(s) 4462
BfmI CTRYAG 1 cut(s) 295
BfrI CTTAAG 6 cut(s) 1931, 2273, 2615, 2957, 3299, 3641
BfuAI ACCTGC 5 cut(s) 541, 2031, 3741, 3793, 4503
BfuI GTATCC 2 cut(s) 160, 1762
BglI GCCNNNNNGGC 1 cut(s) 4128
BglII AGATCT 5 cut(s) 106, 1677, 3835, 4472, 4550
BisI GCNGC 4 cut(s) 295, 298, 3843, 4581
BlpI GCTNAGC 1 cut(s) 525
BlsI GCNGC 4 cut(s) 296, 299, 3844, 4582
BmcAI AGTACT 2 cut(s) 892, 3997
Bme1390I CCNGG 3 cut(s) 462, 737, 1212
Bme18I GGWCC 3 cut(s) 328, 914, 4229
BmeRI GACNNNNNGTC 5 cut(s) 2459, 2801, 3143, 3485, 3917
BmeT110I CYCGRG 6 cut(s) 1808, 2170, 2512, 2854, 3196, 3538
BmgT120I GGNCC 4 cut(s) 328, 914, 1539, 4229
BmiI GGNNCC 7 cut(s) 366, 480, 741, 915, 916, 4230, 4759
BmrFI CCNGG 3 cut(s) 462, 737, 1212
BmrI ACTGGG 6 cut(s) 1298, 2381, 2723, 3065, 3407, 3749
BmsI GCATC 7 cut(s) 1131, 1324, 2355, 2697, 4389, 4648, 4776
BmtI GCTAGC 2 cut(s) 498, 1397
BmuI ACTGGG 6 cut(s) 1298, 2381, 2723, 3065, 3407, 3749
BpiI GAAGAC 2 cut(s) 1710, 3609
BplI GAGNNNNNCTC 6 cut(s) 1216, 1248, 1822, 1854, 1824, 1856
BpmI CTGGAG 2 cut(s) 1454, 1576
Bpu10I CCTNAGC 3 cut(s) 1399, 4623, 4763
Bpu1102I GCTNAGC 1 cut(s) 525
BpuEI CTTGAG 5 cut(s) 361, 845, 959, 1753, 4835
BsaI GGTCTC 7 cut(s) 1429, 1827, 2171, 2513, 2855, 3197, 3539
Bse21I CCTNAGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 103
BseBI CCWGG 3 cut(s) 462, 737, 1212
BseMI GCAATG 1 cut(s) 103
BseRI GAGGAG 4 cut(s) 414, 490, 493, 849
BseXI GCAGC 3 cut(s) 281, 309, 4592
BsgI GTGCAG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 430
BshFI GGCC 3 cut(s) 1541, 3842, 4699
BshNI GGYRCC 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 424
BsiHKCI CYCGRG 6 cut(s) 1808, 2170, 2512, 2854, 3196, 3538
BslFI GGGAC 9 cut(s) 652, 718, 927, 1576, 2466, 2808, 3492, 3924, 4242
BsmBI CGTCTC 1 cut(s) 4702
BsmFI GGGAC 9 cut(s) 652, 718, 927, 1576, 2466, 2808, 3492, 3924, 4242
BsmI GAATGC 1 cut(s) 4345
BsnI GGCC 3 cut(s) 1541, 3842, 4699
Bso31I GGTCTC 7 cut(s) 1429, 1827, 2171, 2513, 2855, 3197, 3539
BsoBI CYCGRG 6 cut(s) 1808, 2170, 2512, 2854, 3196, 3538
Bsp1286I GDGCHC 2 cut(s) 424, 1295
Bsp1407I TGTACA 2 cut(s) 1187, 1248
Bsp1720I GCTNAGC 1 cut(s) 525
Bsp19I CCATGG 2 cut(s) 2439, 2781
BspACI CCGC 6 cut(s) 428, 430, 1382, 1766, 3843, 3949
BspANI GGCC 3 cut(s) 1541, 3842, 4699
BspFNI CGCG 1 cut(s) 430
BspHI TCATGA 2 cut(s) 678, 1095
BspLI GGNNCC 7 cut(s) 366, 480, 741, 915, 916, 4230, 4759
BspMAI CTGCAG 1 cut(s) 299
BspMI ACCTGC 5 cut(s) 541, 2031, 3741, 3793, 4503
BspOI GCTAGC 2 cut(s) 498, 1397
BspT107I GGYRCC 1 cut(s) 478
BspTI CTTAAG 6 cut(s) 1931, 2273, 2615, 2957, 3299, 3641
BspTNI GGTCTC 7 cut(s) 1429, 1827, 2171, 2513, 2855, 3197, 3539
BsrDI GCAATG 1 cut(s) 103
BsrGI TGTACA 2 cut(s) 1187, 1248
BssSI CACGAG 1 cut(s) 4701
BssT1I CCWWGG 8 cut(s) 279, 1474, 2097, 2439, 2781, 3123, 3465, 4083
Bst2BI CACGAG 1 cut(s) 4701
Bst2UI CCWGG 3 cut(s) 462, 737, 1212
Bst6I CTCTTC 4 cut(s) 265, 705, 794, 4293
BstAFI CTTAAG 6 cut(s) 1931, 2273, 2615, 2957, 3299, 3641
BstAPI GCANNNNNTGC 4 cut(s) 2093, 2435, 3119, 4261
BstAUI TGTACA 2 cut(s) 1187, 1248
BstC8I GCNNGC 4 cut(s) 496, 945, 1395, 4616
BstDSI CCRYGG 3 cut(s) 428, 2439, 2781
BstEII GGTNACC 2 cut(s) 2018, 3728
BstENI CCTNNNNNAGG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 430
BstNI CCWGG 3 cut(s) 462, 737, 1212
BstNSI RCATGY 4 cut(s) 258, 294, 1255, 4618
BstPI GGTNACC 2 cut(s) 2018, 3728
BstSCI CCNGG 3 cut(s) 460, 735, 1210
BstSFI CTRYAG 1 cut(s) 295
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 3 cut(s) 281, 309, 4592
BstV2I GAAGAC 2 cut(s) 1710, 3609
BstXI CCANNNNNNTGG 1 cut(s) 1549
Bsu36I CCTNAGG 1 cut(s) 505
BsuI GTATCC 2 cut(s) 160, 1762
BsuRI GGCC 3 cut(s) 1541, 3842, 4699
BtgI CCRYGG 3 cut(s) 428, 2439, 2781
BtgZI GCGATG 1 cut(s) 734
BveI ACCTGC 5 cut(s) 541, 2031, 3741, 3793, 4503
Cac8I GCNNGC 4 cut(s) 496, 945, 1395, 4616
CaiI CAGNNNCTG 1 cut(s) 4825
CciI TCATGA 2 cut(s) 678, 1095
Cfr13I GGNCC 4 cut(s) 328, 914, 1539, 4229
Cfr42I CCGCGG 1 cut(s) 431
CseI GACGC 6 cut(s) 545, 2284, 2626, 2968, 3310, 3652
CsiI ACCWGGT 1 cut(s) 1210
Csp6I GTAC 8 cut(s) 43, 152, 891, 1188, 1249, 3996, 4267, 4460
CviQI GTAC 8 cut(s) 43, 152, 891, 1188, 1249, 3996, 4267, 4460
DraI TTTAAA 1 cut(s) 1509
DraIII CACNNNGTG 2 cut(s) 1214, 1536
DriI GACNNNNNGTC 5 cut(s) 2459, 2801, 3143, 3485, 3917
EaeI YGGCCR 2 cut(s) 3840, 4697
Eam1104I CTCTTC 4 cut(s) 265, 705, 794, 4293
Eam1105I GACNNNNNGTC 5 cut(s) 2459, 2801, 3143, 3485, 3917
EarI CTCTTC 4 cut(s) 265, 705, 794, 4293
Eco130I CCWWGG 8 cut(s) 279, 1474, 2097, 2439, 2781, 3123, 3465, 4083
Eco24I GRGCYC 1 cut(s) 1295
Eco31I GGTCTC 7 cut(s) 1429, 1827, 2171, 2513, 2855, 3197, 3539
Eco32I GATATC 3 cut(s) 1481, 4414, 4538
Eco47I GGWCC 3 cut(s) 328, 914, 4229
Eco57I CTGAAG 8 cut(s) 344, 1043, 2024, 2366, 2708, 3050, 3392, 3734
Eco81I CCTNAGG 1 cut(s) 505
Eco88I CYCGRG 6 cut(s) 1808, 2170, 2512, 2854, 3196, 3538
Eco91I GGTNACC 2 cut(s) 2018, 3728
EcoNI CCTNNNNNAGG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 914
EcoO65I GGTNACC 2 cut(s) 2018, 3728
EcoRII CCWGG 3 cut(s) 460, 735, 1210
EcoRV GATATC 3 cut(s) 1481, 4414, 4538
EcoT14I CCWWGG 8 cut(s) 279, 1474, 2097, 2439, 2781, 3123, 3465, 4083
EcoT38I GRGCYC 1 cut(s) 1295
ErhI CCWWGG 8 cut(s) 279, 1474, 2097, 2439, 2781, 3123, 3465, 4083
Esp3I CGTCTC 1 cut(s) 4702
FaqI GGGAC 9 cut(s) 652, 718, 927, 1576, 2466, 2808, 3492, 3924, 4242
FbaI TGATCA 3 cut(s) 376, 1417, 4390
FblI GTMKAC 2 cut(s) 228, 3967
Fnu4HI GCNGC 4 cut(s) 295, 298, 3843, 4581
FriOI GRGCYC 1 cut(s) 1295
Fsp4HI GCNGC 4 cut(s) 295, 298, 3843, 4581
GluI GCNGC 4 cut(s) 295, 298, 3843, 4581
GsuI CTGGAG 2 cut(s) 1454, 1576
HaeIII GGCC 3 cut(s) 1541, 3842, 4699
HgaI GACGC 6 cut(s) 545, 2284, 2626, 2968, 3310, 3652
HincII GTYRAC 1 cut(s) 1354
HindII GTYRAC 1 cut(s) 1354
HindIII AAGCTT 4 cut(s) 809, 1661, 3973, 4666
Hpy166II GTNNAC 6 cut(s) 45, 229, 872, 1354, 1858, 3968
Hpy8I GTNNAC 6 cut(s) 45, 229, 872, 1354, 1858, 3968
Hpy99I CGWCG 2 cut(s) 1062, 1526
HpyCH4IV ACGT 2 cut(s) 160, 372
HpySE526I ACGT 2 cut(s) 160, 372
KflI GGGWCCC 1 cut(s) 914
Ksp22I TGATCA 3 cut(s) 376, 1417, 4390
KspI CCGCGG 1 cut(s) 431
Lsp1109I GCAGC 3 cut(s) 281, 309, 4592
LweI GCATC 7 cut(s) 1131, 1324, 2355, 2697, 4389, 4648, 4776
MabI ACCWGGT 1 cut(s) 1210
MaeII ACGT 2 cut(s) 160, 372
MfeI CAATTG 3 cut(s) 1309, 1322, 1626
MhlI GDGCHC 2 cut(s) 424, 1295
MlsI TGGCCA 1 cut(s) 4699
MluNI TGGCCA 1 cut(s) 4699
MlyI GAGTC 9 cut(s) 1886, 1921, 2228, 2263, 2605, 2947, 3254, 3289, 3596
Mox20I TGGCCA 1 cut(s) 4699
MroXI GAANNNNTTC 2 cut(s) 1367, 4669
MscI TGGCCA 1 cut(s) 4699
MslI CAYNNNNRTG 1 cut(s) 3983
Msp20I TGGCCA 1 cut(s) 4699
MspA1I CMGCKG 2 cut(s) 430, 3845
MspCI CTTAAG 6 cut(s) 1931, 2273, 2615, 2957, 3299, 3641
MspR9I CCNGG 3 cut(s) 462, 737, 1212
MunI CAATTG 3 cut(s) 1309, 1322, 1626
Mva1269I GAATGC 1 cut(s) 4345
MvaI CCWGG 3 cut(s) 462, 737, 1212
MvnI CGCG 1 cut(s) 430
NcoI CCATGG 2 cut(s) 2439, 2781
NheI GCTAGC 2 cut(s) 494, 1393
NlaIV GGNNCC 7 cut(s) 366, 480, 741, 915, 916, 4230, 4759
NspI RCATGY 4 cut(s) 258, 294, 1255, 4618
PaeI GCATGC 1 cut(s) 4618
PaeR7I CTCGAG 1 cut(s) 1808
PagI TCATGA 2 cut(s) 678, 1095
PaqCI CACCTGC 1 cut(s) 541
PciI ACATGT 1 cut(s) 254
PctI GAATGC 1 cut(s) 4345
PdmI GAANNNNTTC 2 cut(s) 1367, 4669
PflMI CCANNNNNTGG 9 cut(s) 309, 2191, 2533, 2875, 3106, 3217, 3448, 3559, 3880
PkrI GCNGC 4 cut(s) 296, 299, 3844, 4582
PleI GAGTC 9 cut(s) 1885, 1921, 2227, 2263, 2605, 2947, 3253, 3289, 3595
PpsI GAGTC 9 cut(s) 1885, 1921, 2227, 2263, 2605, 2947, 3253, 3289, 3595
PpuMI RGGWCCY 1 cut(s) 914
PscI ACATGT 1 cut(s) 254
Psp1406I AACGTT 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 3 cut(s) 460, 735, 1210
PspEI GGTNACC 2 cut(s) 2018, 3728
PspGI CCWGG 3 cut(s) 460, 735, 1210
PspN4I GGNNCC 7 cut(s) 366, 480, 741, 915, 916, 4230, 4759
PspPI GGNCC 4 cut(s) 328, 914, 1539, 4229
PspPPI RGGWCCY 1 cut(s) 914
PspXI VCTCGAGB 1 cut(s) 1808
PstI CTGCAG 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 4825
RsaI GTAC 8 cut(s) 44, 153, 892, 1189, 1250, 3997, 4268, 4461
RsaNI GTAC 8 cut(s) 43, 152, 891, 1188, 1249, 3996, 4267, 4460
RseI CAYNNNNRTG 1 cut(s) 3983
SacII CCGCGG 1 cut(s) 431
SatI GCNGC 4 cut(s) 295, 298, 3843, 4581
Sau96I GGNCC 4 cut(s) 328, 914, 1539, 4229
ScaI AGTACT 2 cut(s) 892, 3997
SchI GAGTC 9 cut(s) 1886, 1921, 2228, 2263, 2605, 2947, 3254, 3289, 3596
ScrFI CCNGG 3 cut(s) 462, 737, 1212
SduI GDGCHC 2 cut(s) 424, 1295
SexAI ACCWGGT 1 cut(s) 1210
SfaNI GCATC 7 cut(s) 1131, 1324, 2355, 2697, 4389, 4648, 4776
SfcI CTRYAG 1 cut(s) 295
Sfr274I CTCGAG 1 cut(s) 1808
Sfr303I CCGCGG 1 cut(s) 431
SgrBI CCGCGG 1 cut(s) 431
SinI GGWCC 3 cut(s) 328, 914, 4229
SlaI CTCGAG 1 cut(s) 1808
SmiMI CAYNNNNRTG 1 cut(s) 3983
SpeI ACTAGT 1 cut(s) 4462
SphI GCATGC 1 cut(s) 4618
SsiI CCGC 6 cut(s) 428, 430, 1382, 1766, 3843, 3949
SspI AATATT 2 cut(s) 1066, 4378
StyD4I CCNGG 3 cut(s) 460, 735, 1210
StyI CCWWGG 8 cut(s) 279, 1474, 2097, 2439, 2781, 3123, 3465, 4083
TaiI ACGT 2 cut(s) 163, 375
TaqI TCGA 9 cut(s) 957, 1223, 1467, 1501, 1809, 3600, 4200, 4508, 4566
TatI WGTACW 6 cut(s) 42, 890, 1187, 1248, 3995, 4266
TauI GCSGC 1 cut(s) 3845
TseI GCWGC 3 cut(s) 294, 297, 4580
TspGWI ACGGA 3 cut(s) 320, 4726, 4784
Van91I CCANNNNNTGG 9 cut(s) 309, 2191, 2533, 2875, 3106, 3217, 3448, 3559, 3880
Vha464I CTTAAG 6 cut(s) 1931, 2273, 2615, 2957, 3299, 3641
VpaK11BI GGWCC 3 cut(s) 328, 914, 4229
XagI CCTNNNNNAGG 1 cut(s) 343
XapI RAATTY 6 cut(s) 397, 1150, 1367, 1825, 3456, 4450
XbaI TCTAGA 3 cut(s) 1237, 3821, 4540
XceI RCATGY 4 cut(s) 258, 294, 1255, 4618
XcmI CCANNNNNNNNNTGG 1 cut(s) 3895
XhoI CTCGAG 1 cut(s) 1808
XmiI GTMKAC 2 cut(s) 228, 3967
XmnI GAANNNNTTC 2 cut(s) 1367, 4669
ZrmI AGTACT 2 cut(s) 892, 3997
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.