FvH4_6g12870

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000166
Physical Location & Seq
Reverse (-)
7802933 .. 7805299
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g12870.t1

Sequence Viewer

Length: 2367 bp
ATGGAAGTTCCTAAATTTCTCATTCTTCTACTTTTTGCCGCAACAGTTGCTTCTTTCCAAACACGGTCACTAGGGCTGCAAAGCTTAAGGCAAGGGAGCTCCTTGGAAGTCGAGGAAGAGAGTGTCTTGGTTTCACCAAAAGGAACTTTCTCTAGTGGATTTTACAACATCAGCACCAATGCCTCTTGCTATTCAGTATGGTACACCAACACTGTCCACAAAACAGTTGTCTGGATGGCTAACAGAGACACACCTGTCAGTGTAAGACAGTCAAAATTAAGCCTCCAGAGAAATGGCAACCTGGTCTTGACTGATGGAGTTGGTTCAACAGTATGGTCAACTAACACTTTATCTAATGCAAGCATGGAGTTTCACCTTCGACTTCTTGAAACCGGAAATTTGGTACTGCTCAACCAAGCAGAGAGGATCATCTGGCAGAGTTTTGATTCTCCCACAGACACACTTTTACCATCGCAGCAATTTGACAAGAGCAAAACCTTGGTGTCCATGAGAAGTCGAGGTACATACTTGTCTGGTTACTATAATTTCAAATTTGATGGTAATAATGTGTTGTACCTCACCCACGATAGCCCTCTAGTGTCAAGTGTTTATTGGCCAAAGATGGATGTGTCTGTTTTTGGTAGTGGCAGATCACCTTACAATAGCTCTAGAGTAGCAGTATTGGATGAACGAGGAAAATTTGTATCAAGTGACAATTTAAAGTTCAAAGCATCTGACTATGGGGTTGGTCCAAAGCGCCGTCTAACCTTGGATTATGATGGCCTCATAAGAATGTACAGTCTAGATGAATCAACTGGACTTTGGAAAGTCTCATGGTTGCCTGAAATTGATTATTGCCGGGTTCATGGCTTATGTGGTGAGTATGGTATTTGCACTTACAAACCAGAACCAACTTGTACTTGCCCTCCTGGTTTTTCCCTGACTGATCCTTCAGACTGGTCCAAAGGCTGCTCACCTCCATACAATTTGTCTCATGACTCAAAAAAGTTGGACTTCATGGAGCTTCCTTACACAGATTACTACGGATCTGATTTGGACACCTATCATATGGGAATCTCTTTCGAAGAATGCAGGAATACATGCCTGAATGATACAAGGTGCAAAGGGTTTGGATTTGCACTAGACGGATTTGGACAGTGTTACCCTAAGAACTTTCTTCTTAATGGATTTCGTGTGCCAAGTACTCCCAGCATCATCCATATTAAGATTTTGAAAGCTTCACTGAGTCCTCAAGTACTCCAGCCAGAGACATATGAGTTGAACTGTTCCAGGGCAGAAGTTGCTTATAAAAGTAGTGGTCCTCGAGTGAAAAAGAGCAATCAGTATATTAAGTACCTGATTGGCTTTGTAACTTCTTTTGCAATATTTGAAGCCATCTGCATTGGCTTGACATGGTGGTGCGTGTTGCGAAAGCATGCTGGTGAGGACTATGTGAGCATTGGTTACATGGAATTAGTCATGGAAATCAAACGTTTCACCTATGCAGAACTGAAAAGAGCAACCAAGGATTTCACACAGGAGATAGGAAAAGGCGGTTTTGGAGCAGTTTATAAAGGGGTTCTTGGCGATGGAAGAGTTGTTGCTGTGAAGAGACTAGAAGGCATCGTGCAAGGAGATGCAGAGTTTTGGGCAGAGGTTAGTGTTATAGGGAATATCAACCACAGAAACTTGGTTAAGCTTTGGGGTTTCTGTGCTGAGAATGAACATAAGCTATTGGTGTATGAGTACCATGAGAATGGATCCTTGGATAAAATCTTGTTCTCAGATGTGGAATTAGGATTGGAGCAGCGATACAACATTGCATTAGGAACTGCAAAGGGTTTGTCATATTTGCACGAGGAGTGCTTGGAATGGGTGCTCCACTGTGATGTAAAGCCTCAGAACATTCTTTTAGATGACCAACTTGAACCAAAAGTGGCAGACTTTGGCATGTCAAAGTTGTTCAAAGACATTCCTGATGACACTGGGTTTTCAAGAGCTCGCGGCACCAGAGGTTACATGGCTCCAGAGTGGATGATGAATCTGAAGATTGATGCAAAGGTAGATGTCTACAGCTACGGGATTGTTTTGTTAGAACTACTGACTGGAAAGAGTGCTTCTATTCTACTTACGCCCCAGGCTAGTCCCCTCCCAAAGAACAAAGAACACAACCAAGTAGTACAATGTGTGATTGAAAAAATCATCAGAGAAGGGCTTGAGGATGTGATTGATCCTAGACTACACCACGAATTCAACAACAAGAAGCTTGAAAGATTGATGAAAGTTGCTCTACTTTGTGTGCATGAAGACCGCAATGCAAGGCCAGCCATGAGTAAGGTTGTGGAGCTTCTAATTCCAAATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

789

Amino Acids

88.89

Weight (kDa)

6.42

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 165 1.4e-26 D-mannose binding lectin
S_locus_glycop PF00954 253 - 313 6.4e-10 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 511 - 784 7.3e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 512 - 781 6e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1308, 1572
AasI GACNNNNNNGTC 1 cut(s) 254
AccB1I GGYRCC 1 cut(s) 2006
AccI GTMKAC 1 cut(s) 2070
AccII CGCG 1 cut(s) 2004
AciI CCGC 4 cut(s) 39, 1554, 2004, 2311
AclI AACGTT 1 cut(s) 1492
AclWI GGATC 6 cut(s) 434, 941, 1054, 1755, 1768, 2225
AcoI YGGCCR 1 cut(s) 614
AcsI RAATTY 5 cut(s) 14, 397, 551, 698, 2249
AcuI CTGAAG 2 cut(s) 936, 2066
AflII CTTAAG 1 cut(s) 85
AjnI CCWGG 4 cut(s) 300, 928, 1289, 2136
AjuI GAANNNNNNNTTGG 2 cut(s) 1748, 1780
Alw21I GWGCWC 3 cut(s) 101, 1881, 2002
Alw26I GTCTC 5 cut(s) 240, 835, 996, 1262, 1606
AlwI GGATC 6 cut(s) 434, 941, 1054, 1755, 1768, 2225
Ama87I CYCGRG 1 cut(s) 1323
AoxI GGCC 3 cut(s) 614, 781, 2321
ApeKI GCWGC 4 cut(s) 76, 475, 969, 1807
ApoI RAATTY 5 cut(s) 14, 397, 551, 698, 2249
ArsI GACNNNNNNTTYG 4 cut(s) 213, 245, 1829, 1861
AspLEI GCGC 1 cut(s) 759
AspS9I GGNCC 3 cut(s) 749, 960, 1319
AsuC2I CCSGG 1 cut(s) 860
AsuHPI GGTGA 8 cut(s) 126, 365, 571, 645, 890, 966, 1454, 1489
AsuII TTCGAA 1 cut(s) 1083
AvaI CYCGRG 1 cut(s) 1323
AvaII GGWCC 3 cut(s) 749, 960, 1319
BalI TGGCCA 1 cut(s) 616
BamHI GGATCC 1 cut(s) 1760
BanI GGYRCC 1 cut(s) 2006
BanII GRGCYC 2 cut(s) 101, 2002
BarI GAAGNNNNNNTAC 2 cut(s) 505, 537
BauI CACGAG 1 cut(s) 1856
BbsI GAAGAC 1 cut(s) 2313
Bbv12I GWGCWC 3 cut(s) 101, 1881, 2002
BbvI GCAGC 4 cut(s) 63, 487, 956, 1819
BccI CCATC 8 cut(s) 229, 308, 478, 551, 616, 773, 1403, 1583
BceAI ACGGC 1 cut(s) 744
BciT130I CCWGG 4 cut(s) 302, 930, 1291, 2138
BcnI CCSGG 1 cut(s) 860
BcoDI GTCTC 5 cut(s) 240, 835, 996, 1262, 1606
BfaI CTAG 9 cut(s) 71, 153, 596, 669, 803, 1142, 1616, 2142, 2235
BfmI CTRYAG 1 cut(s) 2071
BfoI RGCGCY 1 cut(s) 760
BfrI CTTAAG 1 cut(s) 85
BisI GCNGC 6 cut(s) 39, 77, 476, 970, 1808, 2005
BlsI GCNGC 6 cut(s) 40, 78, 477, 971, 1809, 2006
BmcAI AGTACT 2 cut(s) 1204, 1257
Bme1390I CCNGG 5 cut(s) 302, 860, 930, 1291, 2138
Bme18I GGWCC 3 cut(s) 749, 960, 1319
BmeT110I CYCGRG 1 cut(s) 1323
BmgT120I GGNCC 3 cut(s) 749, 960, 1319
BmiI GGNNCC 3 cut(s) 1762, 2008, 2025
BmrFI CCNGG 5 cut(s) 302, 860, 930, 1291, 2138
BmrI ACTGGG 1 cut(s) 1995
BmsI GCATC 5 cut(s) 740, 1221, 1627, 1632, 2044
BmuI ACTGGG 1 cut(s) 1995
BpiI GAAGAC 1 cut(s) 2313
BpmI CTGGAG 3 cut(s) 269, 1244, 2010
Bpu14I TTCGAA 1 cut(s) 1083
BpuEI CTTGAG 2 cut(s) 1236, 2237
BpuMI CCSGG 1 cut(s) 860
BsaJI CCNNGG 7 cut(s) 102, 498, 768, 1290, 1524, 1764, 2136
BsaWI WCCGGW 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 910, 940
Bse1I ACTGG 4 cut(s) 820, 962, 1990, 2110
Bse3DI GCAATG 2 cut(s) 1818, 2320
BseBI CCWGG 4 cut(s) 302, 930, 1291, 2138
BseDI CCNNGG 7 cut(s) 102, 498, 768, 1290, 1524, 1764, 2136
BseGI GGATG 6 cut(s) 240, 631, 691, 1215, 2040, 2227
BseMI GCAATG 2 cut(s) 1818, 2320
BseMII CTCAG 4 cut(s) 1235, 1707, 1797, 1913
BseNI ACTGG 4 cut(s) 820, 962, 1990, 2110
BseRI GAGGAG 1 cut(s) 1874
BseXI GCAGC 4 cut(s) 63, 487, 956, 1819
BseYI CCCAGC 1 cut(s) 1208
Bsh1236I CGCG 1 cut(s) 2004
BshFI GGCC 3 cut(s) 616, 783, 2323
BshNI GGYRCC 1 cut(s) 2006
BsiHKAI GWGCWC 3 cut(s) 101, 1881, 2002
BsiHKCI CYCGRG 1 cut(s) 1323
BsiSI CCGG 2 cut(s) 393, 859
BslFI GGGAC 1 cut(s) 2130
BsmAI GTCTC 5 cut(s) 240, 835, 996, 1262, 1606
BsmFI GGGAC 1 cut(s) 2130
BsmI GAATGC 1 cut(s) 1094
BsnI GGCC 3 cut(s) 616, 783, 2323
BsoBI CYCGRG 1 cut(s) 1323
Bsp119I TTCGAA 1 cut(s) 1083
Bsp1286I GDGCHC 3 cut(s) 101, 1881, 2002
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
BspACI CCGC 4 cut(s) 39, 1554, 2004, 2311
BspANI GGCC 3 cut(s) 616, 783, 2323
BspCNI CTCAG 4 cut(s) 1236, 1708, 1796, 1912
BspFNI CGCG 1 cut(s) 2004
BspHI TCATGA 1 cut(s) 994
BspLI GGNNCC 3 cut(s) 1762, 2008, 2025
BspPI GGATC 6 cut(s) 434, 941, 1054, 1755, 1768, 2225
BspT104I TTCGAA 1 cut(s) 1083
BspT107I GGYRCC 1 cut(s) 2006
BspTI CTTAAG 1 cut(s) 85
BsrDI GCAATG 2 cut(s) 1818, 2320
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 4 cut(s) 820, 962, 1990, 2110
BssECI CCNNGG 7 cut(s) 102, 498, 768, 1290, 1524, 1764, 2136
BssMI GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
BssSI CACGAG 1 cut(s) 1856
BssT1I CCWWGG 5 cut(s) 102, 498, 768, 1524, 1764
Bst2BI CACGAG 1 cut(s) 1856
Bst2UI CCWGG 4 cut(s) 302, 930, 1291, 2138
Bst6I CTCTTC 3 cut(s) 111, 1588, 1604
BstAFI CTTAAG 1 cut(s) 85
BstAPI GCANNNNNTGC 2 cut(s) 47, 1301
BstAUI TGTACA 1 cut(s) 795
BstBI TTCGAA 1 cut(s) 1083
BstC8I GCNNGC 4 cut(s) 361, 1437, 2002, 2325
BstDEI CTNAG 5 cut(s) 1167, 1244, 1716, 1783, 1899
BstF5I GGATG 6 cut(s) 240, 631, 691, 1215, 2040, 2227
BstFNI CGCG 1 cut(s) 2004
BstH2I RGCGCY 1 cut(s) 760
BstHHI GCGC 1 cut(s) 759
BstKTI GATC 6 cut(s) 429, 653, 949, 1049, 1763, 2233
BstMAI GTCTC 5 cut(s) 240, 835, 996, 1262, 1606
BstMBI GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
BstMWI GCNNNNNNNGC 3 cut(s) 47, 1301, 2324
BstNI CCWGG 4 cut(s) 302, 930, 1291, 2138
BstNSI RCATGY 3 cut(s) 1104, 1439, 1954
BstSCI CCNGG 5 cut(s) 300, 858, 928, 1289, 2136
BstSFI CTRYAG 1 cut(s) 2071
BstUI CGCG 1 cut(s) 2004
BstV1I GCAGC 4 cut(s) 63, 487, 956, 1819
BstV2I GAAGAC 1 cut(s) 2313
BstX2I RGATCY 2 cut(s) 1046, 1760
BstXI CCANNNNNNTGG 2 cut(s) 293, 1757
BstYI RGATCY 2 cut(s) 1046, 1760
BsuRI GGCC 3 cut(s) 616, 783, 2323
BtgZI GCGATG 2 cut(s) 456, 1602
BtsCI GGATG 6 cut(s) 240, 631, 691, 1215, 2040, 2227
BtsIMutI CAGTG 6 cut(s) 210, 265, 1163, 1241, 1882, 1983
Cac8I GCNNGC 4 cut(s) 361, 1437, 2002, 2325
CciI TCATGA 1 cut(s) 994
CfoI GCGC 1 cut(s) 759
Cfr13I GGNCC 3 cut(s) 749, 960, 1319
CsiI ACCWGGT 1 cut(s) 300
DdeI CTNAG 5 cut(s) 1167, 1244, 1716, 1783, 1899
DpnI GATC 6 cut(s) 428, 652, 948, 1048, 1762, 2232
DpnII GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
DraI TTTAAA 1 cut(s) 720
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 614
Eam1104I CTCTTC 3 cut(s) 111, 1588, 1604
EarI CTCTTC 3 cut(s) 111, 1588, 1604
Ecl136II GAGCTC 2 cut(s) 99, 2000
Eco130I CCWWGG 5 cut(s) 102, 498, 768, 1524, 1764
Eco24I GRGCYC 2 cut(s) 101, 2002
Eco47I GGWCC 3 cut(s) 749, 960, 1319
Eco53kI GAGCTC 2 cut(s) 99, 2000
Eco57I CTGAAG 2 cut(s) 936, 2066
Eco88I CYCGRG 1 cut(s) 1323
EcoICRI GAGCTC 2 cut(s) 99, 2000
EcoRI GAATTC 1 cut(s) 2249
EcoRII CCWGG 4 cut(s) 300, 928, 1289, 2136
EcoT14I CCWWGG 5 cut(s) 102, 498, 768, 1524, 1764
EcoT38I GRGCYC 2 cut(s) 101, 2002
ErhI CCWWGG 5 cut(s) 102, 498, 768, 1524, 1764
FalI AAGNNNNNCTT 4 cut(s) 998, 1030, 1566, 1598
FaqI GGGAC 1 cut(s) 2130
FauNDI CATATG 2 cut(s) 1068, 1273
FblI GTMKAC 1 cut(s) 2070
Fnu4HI GCNGC 6 cut(s) 39, 77, 476, 970, 1808, 2005
FokI GGATG 6 cut(s) 247, 638, 698, 1202, 2047, 2234
FriOI GRGCYC 2 cut(s) 101, 2002
Fsp4HI GCNGC 6 cut(s) 39, 77, 476, 970, 1808, 2005
FspBI CTAG 9 cut(s) 71, 153, 596, 669, 803, 1142, 1616, 2142, 2235
GlaI GCGC 1 cut(s) 758
GluI GCNGC 6 cut(s) 39, 77, 476, 970, 1808, 2005
GsaI CCCAGC 1 cut(s) 1212
GsuI CTGGAG 3 cut(s) 269, 1244, 2010
HaeII RGCGCY 1 cut(s) 760
HaeIII GGCC 3 cut(s) 616, 783, 2323
HapII CCGG 2 cut(s) 393, 859
HhaI GCGC 1 cut(s) 759
Hin6I GCGC 1 cut(s) 757
HinP1I GCGC 1 cut(s) 757
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 4 cut(s) 82, 1236, 1697, 2264
HinfI GANTC 6 cut(s) 446, 809, 998, 1074, 1246, 2041
HpaII CCGG 2 cut(s) 393, 859
HphI GGTGA 8 cut(s) 126, 365, 571, 645, 890, 966, 1454, 1489
Hpy166II GTNNAC 4 cut(s) 204, 217, 339, 2071
Hpy188I TCNGA 7 cut(s) 736, 955, 1051, 1786, 1902, 2046, 2207
Hpy8I GTNNAC 4 cut(s) 204, 217, 339, 2071
HpyAV CCTTC 4 cut(s) 386, 960, 1613, 2204
HpyCH4IV ACGT 1 cut(s) 1492
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 1301, 2324
HpyF3I CTNAG 5 cut(s) 1167, 1244, 1716, 1783, 1899
HpySE526I ACGT 1 cut(s) 1492
HspAI GCGC 1 cut(s) 757
Kzo9I GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
LmnI GCTCC 8 cut(s) 96, 104, 1021, 1562, 1804, 1884, 2029, 2344
Lsp1109I GCAGC 4 cut(s) 63, 487, 956, 1819
LweI GCATC 5 cut(s) 740, 1221, 1627, 1632, 2044
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 9 cut(s) 71, 153, 596, 669, 803, 1142, 1616, 2142, 2235
MaeII ACGT 1 cut(s) 1492
MaeIII GTNAC 7 cut(s) 66, 536, 710, 1160, 1369, 1463, 2015
MalI GATC 6 cut(s) 428, 652, 948, 1048, 1762, 2232
MboI GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
MboII GAAGA 8 cut(s) 17, 128, 1097, 1169, 1605, 1621, 2059, 2318
MflI RGATCY 2 cut(s) 1046, 1760
MhlI GDGCHC 3 cut(s) 101, 1881, 2002
MlsI TGGCCA 1 cut(s) 616
MluNI TGGCCA 1 cut(s) 616
MlyI GAGTC 2 cut(s) 992, 1255
MmeI TCCRAC 1 cut(s) 990
Mox20I TGGCCA 1 cut(s) 616
MscI TGGCCA 1 cut(s) 616
MseI TTAA 7 cut(s) 86, 278, 719, 1182, 1224, 1350, 1695
MslI CAYNNNNRTG 5 cut(s) 791, 1417, 1440, 1755, 1887
Msp20I TGGCCA 1 cut(s) 616
MspCI CTTAAG 1 cut(s) 85
MspI CCGG 2 cut(s) 393, 859
MspR9I CCNGG 5 cut(s) 302, 860, 930, 1291, 2138
Mva1269I GAATGC 1 cut(s) 1094
MvaI CCWGG 4 cut(s) 302, 930, 1291, 2138
MvnI CGCG 1 cut(s) 2004
MwoI GCNNNNNNNGC 3 cut(s) 47, 1301, 2324
NciI CCSGG 1 cut(s) 860
NdeI CATATG 2 cut(s) 1068, 1273
NdeII GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
NlaIV GGNNCC 3 cut(s) 1762, 2008, 2025
NmuCI GTSAC 2 cut(s) 66, 710
NspI RCATGY 3 cut(s) 1104, 1439, 1954
NspV TTCGAA 1 cut(s) 1083
PaeI GCATGC 1 cut(s) 1439
PaeR7I CTCGAG 1 cut(s) 1323
PagI TCATGA 1 cut(s) 994
PctI GAATGC 1 cut(s) 1094
PfeI GAWTC 4 cut(s) 446, 809, 1074, 2041
PkrI GCNGC 6 cut(s) 40, 78, 477, 971, 1809, 2006
PleI GAGTC 2 cut(s) 992, 1254
PpsI GAGTC 2 cut(s) 992, 1254
PsiI TTATAA 2 cut(s) 1308, 1572
Psp124BI GAGCTC 2 cut(s) 101, 2002
Psp1406I AACGTT 1 cut(s) 1492
Psp6I CCWGG 4 cut(s) 300, 928, 1289, 2136
PspFI CCCAGC 1 cut(s) 1208
PspGI CCWGG 4 cut(s) 300, 928, 1289, 2136
PspN4I GGNNCC 3 cut(s) 1762, 2008, 2025
PspPI GGNCC 3 cut(s) 749, 960, 1319
PspXI VCTCGAGB 1 cut(s) 1323
PsuI RGATCY 2 cut(s) 1046, 1760
RseI CAYNNNNRTG 5 cut(s) 791, 1417, 1440, 1755, 1887
SacI GAGCTC 2 cut(s) 101, 2002
SaqAI TTAA 7 cut(s) 86, 278, 719, 1182, 1224, 1350, 1695
SatI GCNGC 6 cut(s) 39, 77, 476, 970, 1808, 2005
Sau3AI GATC 6 cut(s) 426, 650, 946, 1046, 1760, 2230
Sau96I GGNCC 3 cut(s) 749, 960, 1319
ScaI AGTACT 2 cut(s) 1204, 1257
SchI GAGTC 2 cut(s) 992, 1255
ScrFI CCNGG 5 cut(s) 302, 860, 930, 1291, 2138
SduI GDGCHC 3 cut(s) 101, 1881, 2002
SexAI ACCWGGT 1 cut(s) 300
SfaNI GCATC 5 cut(s) 740, 1221, 1627, 1632, 2044
SfcI CTRYAG 1 cut(s) 2071
Sfr274I CTCGAG 1 cut(s) 1323
SfuI TTCGAA 1 cut(s) 1083
SinI GGWCC 3 cut(s) 749, 960, 1319
SlaI CTCGAG 1 cut(s) 1323
SmiMI CAYNNNNRTG 5 cut(s) 791, 1417, 1440, 1755, 1887
SmlI CTYRAG 4 cut(s) 85, 1251, 1323, 2216
SmoI CTYRAG 4 cut(s) 85, 1251, 1323, 2216
SphI GCATGC 1 cut(s) 1439
SsiI CCGC 4 cut(s) 39, 1554, 2004, 2311
SspI AATATT 1 cut(s) 1386
SspMI CTAG 9 cut(s) 71, 153, 596, 669, 803, 1142, 1616, 2142, 2235
SstI GAGCTC 2 cut(s) 101, 2002
StyD4I CCNGG 5 cut(s) 300, 858, 928, 1289, 2136
StyI CCWWGG 5 cut(s) 102, 498, 768, 1524, 1764
TaiI ACGT 1 cut(s) 1495
TaqI TCGA 5 cut(s) 111, 379, 517, 1083, 1324
TatI WGTACW 5 cut(s) 795, 917, 1202, 1255, 2179
TauI GCSGC 2 cut(s) 41, 2007
TfiI GAWTC 4 cut(s) 446, 809, 1074, 2041
Tru1I TTAA 7 cut(s) 86, 278, 719, 1182, 1224, 1350, 1695
Tru9I TTAA 7 cut(s) 86, 278, 719, 1182, 1224, 1350, 1695
TscAI CASTG 6 cut(s) 217, 265, 1163, 1248, 1889, 1990
TseFI GTSAC 2 cut(s) 66, 710
TseI GCWGC 4 cut(s) 76, 475, 969, 1807
Tsp45I GTSAC 2 cut(s) 66, 710
TspDTI ATGAA 8 cut(s) 702, 822, 854, 1006, 1737, 2054, 2294, 2319
TspGWI ACGGA 2 cut(s) 1059, 1161
TspRI CASTG 6 cut(s) 217, 265, 1163, 1248, 1889, 1990
Vha464I CTTAAG 1 cut(s) 85
VpaK11BI GGWCC 3 cut(s) 749, 960, 1319
XapI RAATTY 5 cut(s) 14, 397, 551, 698, 2249
XbaI TCTAGA 2 cut(s) 668, 802
XceI RCATGY 3 cut(s) 1104, 1439, 1954
XcmI CCANNNNNNNNNTGG 1 cut(s) 2017
XhoI CTCGAG 1 cut(s) 1323
XmiI GTMKAC 1 cut(s) 2070
XspI CTAG 9 cut(s) 71, 153, 596, 669, 803, 1142, 1616, 2142, 2235
ZrmI AGTACT 2 cut(s) 1204, 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.