MD12G1151600.v1.1

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000166
Physical Location & Seq
Forward (+)
23134350 .. 23136731
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1151600.v1.1.491

Sequence Viewer

Length: 2382 bp
ATGGAACTTCCCAAGTTTCTTATTCTGTTTTTGTTTGCTGCCACAGTGGCTTGGAGTCAACAGCAATTTCCGGGGCTTCCGAGCTTAAGGCAGGGAAGCTCCTTAACAGTTGAGAAGGAGAGCGACTTGTTGGTTTCACCAAATGGAACTTTCTCAAGTGGATTTTACAAGGTGGGCACTGATGCCTATTGCTATGCAATATGGTTTACCAATTCCGCCAATAAAACAGTTGCATGGATGGCTAACAGAGATGAGCCCGTTAGCGCACGAGGGTCCAAGCTTACCCTCCACAAAGATGGCAACCTTGTCCTGACTGATGGGGTTGGTTCAACTGTATGGTCAACCAGCACTTTCTCTAATGCAGGCGTGGAGGCTCGGCTTCTTGAGACTGGAAACTTGGTGCTGATCAACCAATCAAAGGGGGTCATATGGCAGAGTTTTGACTTTCCGACGGATACAGTTTTGCCATCACAAAGAATTGTCAAGAAAACAATGTTGGTGTCCATGAGAAGCCAAGGTACATATTTATCTGGATACTACAACTTCAAATTTGATGACACCAACATCCTTTATCTCATCTATAATGGTCCAATCATTTCGAGTGTTTACTGGCCGAGAACAGATGCAACTGTTTTTTATAGCAAAAGAACACCTTATAATAGCTCTAGAATAGCAATTTTGAATGAGGCTGGACAATTCAGATCCAGCGACAATTTGAATTTCAACGCATCTGATTATGGAGTTGGCCCAAAGCGCCGTTTAACAATAGATTATGATGGCATTTTGAGATTGTACAGTTTAGATGAATCAACTCGGCTTTGGGAAATCTCATGGTTGCCGAGTAGTGTTGGAGCTTGCCAAGTTGATGGCTTGTGTGGTGAATATGGTATTTGCATTTACAACCCACATCCTACTTGTACTTGCCCTTATGGTTTCACCAGAAACAATCCTTCAGACTGGTCTAGAGGCTGCTCGCCTTCGTTCAATTTGTCTACCGATTCAAGCAAATTGGATTTCATGGAACTTCCTTACACAGATTACTATGGATACGACTTGGGGACGTACAGGTTTGGCATTTCATTTGAAGCCTGCAGGAATTCATGCCTGAATGCTACAAGATGCAGAGGATTTGGATATGCACTGGATGGACAAGGACAGTGTTACCCTAAATCTCTTCTCCTTAATGGATATCGTTCGCCAACTTCACGCATGATCATACATATTAAGGTTCCAAAGGGATTTCTGAGCCCTCATGCGCTCCCAACGAAGTTTGAAACACATGATTTGAACTGTTCAGGTGCACAAGTTGTTTTCAACAGCAACGATCCTGAAGCAGAGCGCAATAGAAACTGGTACATGAAATACCTAATTGGATTTGTAGGTTCTTTTGCAGTAATTGAAGCACTCTGCGTTGGTTTGACGTGGTGGTATCTCTTCCGAAAGCATGCTCATGAAGAGTTGTCCAATATAGCCGGTTACATGGCATTGGCCATGGACTTCAAACGCTTCACCTATGCAGAACTAACAAGAGCTACCGGTAACTTCAAACAGAAGATAGGGAAAGGGGGCTTCGGAACTGTCTATAAAGGGCTTCTGGATGATGGAAGAGTTGTTGCGGTGAAGAGACTAGAAGGCATCTTACAAGGAGAGGCAGAGTTCTGGGCAGAGGTGAGCGTGATAGGGAATGTGAACCACAGGAACTTGGTCAAACTATGGGGTTTCTGTGCCGAGAATGATCACAAACTGATGGTTTACGAATACCTGAAGAACGGGTCCTTGGATAAAATCTTGTTTTCGGATGTGGAATTTGGATTGGAGAAGCGATACAACATTGCACTAGGCACTGCAAAGGGTTTGTCATATTTACACGAGGAGTGCCTGGAATGGGTACTTCACTGTGATGTGAAGCCTCAAAACATACTGTTAGCCGATGATTTGGAACCTCGGGTGGCAGATTTTGGCATGGCAAAGCTATTCAAAGATATCCACGGCGTCAGAGACAGTCATGGGATTGGCTTTTCACAAGTGCGTGGCACCAGAGGTTATTTAGCTCCAGAGTGGATGATGAACCTGAAAATTGATGCCAAGGCGGACGTGTACAGCTATGGGGTTGTGCTGCTGGAACTACTGAGTGGAAGAAGTGCTTCTAGCTTCCTTTCAACTGGTGGCCAAAACAATGAATACAACCACTTGGTACATTGGGTGACTGAAATGATCGGAGAAGAAGGGCTTGAGAAAGTAATCGACCCTCGGCTACACCATGAGTTCAACACGAAGCTCAAACGATTGATGAAAGTCGCTATGTCGTGCGTACAAGAAGATCGCAAAGCAAGGCCTGCCATGAGTAAGGTTGTGGAGCTTCTCCTTGAAAATGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

794

Amino Acids

89.27

Weight (kDa)

6.79

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 163 6e-25 D-mannose binding lectin
S_locus_glycop PF00954 252 - 315 2.3e-08 S-locus glycoprotein domain
Pkinase PF00069 515 - 785 5.8e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 515 - 788 2.7e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 657
AccB1I GGYRCC 1 cut(s) 2033
AccI GTMKAC 1 cut(s) 992
AciI CCGC 3 cut(s) 216, 1616, 2090
AclWI GGATC 2 cut(s) 696, 1319
AcoI YGGCCR 3 cut(s) 611, 1488, 2167
AcsI RAATTY 4 cut(s) 548, 718, 1096, 1805
AcuI CTGAAG 3 cut(s) 936, 1350, 1784
AcyI GRCGYC 1 cut(s) 1992
AfaI GTAC 9 cut(s) 520, 794, 919, 1064, 1355, 1890, 2099, 2196, 2313
AfiI CCNNNNNNNGG 2 cut(s) 418, 1885
AflII CTTAAG 1 cut(s) 85
AflIII ACRYGT 1 cut(s) 2094
AgeI ACCGGT 1 cut(s) 1535
AjiI CACGTC 2 cut(s) 1422, 2095
AjnI CCWGG 1 cut(s) 1878
AjuI GAANNNNNNNTTGG 4 cut(s) 479, 511, 1760, 1792
Alw21I GWGCWC 1 cut(s) 1303
Alw26I GTCTC 3 cut(s) 380, 1618, 1992
Alw44I GTGCAC 1 cut(s) 1299
AlwI GGATC 2 cut(s) 696, 1319
Ama87I CYCGRG 1 cut(s) 1944
AoxI GGCC 5 cut(s) 611, 745, 1488, 2167, 2333
ApaLI GTGCAC 1 cut(s) 1299
ApeKI GCWGC 3 cut(s) 38, 969, 2116
ApoI RAATTY 4 cut(s) 548, 718, 1096, 1805
ArsI GACNNNNNNTTYG 2 cut(s) 1841, 1873
AsiGI ACCGGT 1 cut(s) 1535
Asp700I GAANNNNTTC 1 cut(s) 2143
AspLEI GCGC 4 cut(s) 266, 756, 1258, 1341
AspS9I GGNCC 4 cut(s) 273, 587, 746, 1773
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 7 cut(s) 129, 890, 928, 1501, 1630, 1681, 2215
AvaI CYCGRG 1 cut(s) 1944
AvaII GGWCC 3 cut(s) 273, 587, 1773
BaeGI GKGCMC 2 cut(s) 179, 1303
BalI TGGCCA 2 cut(s) 1490, 2169
BanI GGYRCC 1 cut(s) 2033
BanII GRGCYC 2 cut(s) 258, 1250
BarI GAAGNNNNNNTAC 4 cut(s) 502, 534, 1623, 1655
BauI CACGAG 2 cut(s) 267, 1868
Bbv12I GWGCWC 1 cut(s) 1303
BbvI GCAGC 3 cut(s) 25, 956, 2103
BccI CCATC 9 cut(s) 232, 290, 311, 475, 770, 860, 1139, 1595, 1741
BceAI ACGGC 2 cut(s) 741, 2005
BciT130I CCWGG 1 cut(s) 1880
BciVI GTATCC 3 cut(s) 448, 527, 1040
BclI TGATCA 3 cut(s) 405, 1212, 1735
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 3 cut(s) 380, 1618, 1992
BfaI CTAG 5 cut(s) 666, 963, 1628, 1838, 2148
BfmI CTRYAG 1 cut(s) 1090
BfoI RGCGCY 1 cut(s) 757
BfrI CTTAAG 1 cut(s) 85
BfuI GTATCC 3 cut(s) 448, 527, 1040
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 3 cut(s) 39, 970, 2117
BlsI GCNGC 3 cut(s) 40, 971, 2118
Bme1390I CCNGG 2 cut(s) 72, 1880
Bme18I GGWCC 3 cut(s) 273, 587, 1773
BmeT110I CYCGRG 1 cut(s) 1944
BmgBI CACGTC 2 cut(s) 1422, 2095
BmgT120I GGNCC 4 cut(s) 273, 587, 746, 1773
BmiI GGNNCC 5 cut(s) 274, 1230, 1774, 1941, 2035
BmrFI CCNGG 2 cut(s) 72, 1880
BmsI GCATC 6 cut(s) 172, 613, 737, 1109, 1644, 2071
BpmI CTGGAG 1 cut(s) 2037
BpuEI CTTGAG 3 cut(s) 139, 404, 2252
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 1992
BsaJI CCNNGG 8 cut(s) 71, 514, 1491, 1776, 1943, 1987, 2085, 2249
BsaWI WCCGGW 1 cut(s) 1535
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 1885
Bse118I RCCGGY 2 cut(s) 1472, 1535
Bse1I ACTGG 6 cut(s) 394, 614, 962, 1146, 1355, 2167
Bse3DI GCAATG 1 cut(s) 1830
BseBI CCWGG 1 cut(s) 1880
BseDI CCNNGG 8 cut(s) 71, 514, 1491, 1776, 1943, 1987, 2085, 2249
BseGI GGATG 7 cut(s) 243, 564, 907, 1150, 1603, 1804, 2067
BseLI CCNNNNNNNGG 2 cut(s) 418, 1885
BseMI GCAATG 1 cut(s) 1830
BseMII CTCAG 2 cut(s) 1235, 2120
BseNI ACTGG 6 cut(s) 394, 614, 962, 1146, 1355, 2167
BseRI GAGGAG 1 cut(s) 1886
BseSI GKGCMC 2 cut(s) 179, 1303
BseXI GCAGC 3 cut(s) 25, 956, 2103
BshFI GGCC 5 cut(s) 613, 747, 1490, 2169, 2335
BshNI GGYRCC 1 cut(s) 2033
BshTI ACCGGT 1 cut(s) 1535
BsiHKAI GWGCWC 1 cut(s) 1303
BsiHKCI CYCGRG 1 cut(s) 1944
BsiSI CCGG 3 cut(s) 71, 1473, 1536
BslFI GGGAC 1 cut(s) 1072
BslI CCNNNNNNNGG 2 cut(s) 418, 1885
BsmAI GTCTC 3 cut(s) 380, 1618, 1992
BsmFI GGGAC 1 cut(s) 1072
BsmI GAATGC 1 cut(s) 1114
BsnI GGCC 5 cut(s) 613, 747, 1490, 2169, 2335
BsoBI CYCGRG 1 cut(s) 1944
Bsp1286I GDGCHC 4 cut(s) 179, 258, 1250, 1303
Bsp1407I TGTACA 2 cut(s) 792, 2097
Bsp143I GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
Bsp19I CCATGG 1 cut(s) 1491
BspACI CCGC 3 cut(s) 216, 1616, 2090
BspANI GGCC 5 cut(s) 613, 747, 1490, 2169, 2335
BspCNI CTCAG 2 cut(s) 1236, 2121
BspHI TCATGA 1 cut(s) 1450
BspLI GGNNCC 5 cut(s) 274, 1230, 1774, 1941, 2035
BspMAI CTGCAG 1 cut(s) 1094
BspPI GGATC 2 cut(s) 696, 1319
BspT107I GGYRCC 1 cut(s) 2033
BspTI CTTAAG 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 1830
BsrFI RCCGGY 2 cut(s) 1472, 1535
BsrGI TGTACA 2 cut(s) 792, 2097
BsrI ACTGG 6 cut(s) 394, 614, 962, 1146, 1355, 2167
BssAI RCCGGY 2 cut(s) 1472, 1535
BssECI CCNNGG 8 cut(s) 71, 514, 1491, 1776, 1943, 1987, 2085, 2249
BssMI GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
BssNI GRCGYC 1 cut(s) 1992
BssSI CACGAG 2 cut(s) 267, 1868
BssT1I CCWWGG 4 cut(s) 514, 1491, 1776, 2085
Bst2BI CACGAG 2 cut(s) 267, 1868
Bst2UI CCWGG 1 cut(s) 1880
Bst6I CTCTTC 5 cut(s) 1179, 1439, 1449, 1600, 1616
BstACI GRCGYC 1 cut(s) 1992
BstAFI CTTAAG 1 cut(s) 85
BstAPI GCANNNNNTGC 1 cut(s) 2336
BstAUI TGTACA 2 cut(s) 792, 2097
BstC8I GCNNGC 6 cut(s) 364, 856, 974, 1090, 1446, 2337
BstDEI CTNAG 2 cut(s) 1244, 2129
BstDSI CCRYGG 2 cut(s) 1491, 1987
BstF5I GGATG 7 cut(s) 243, 564, 907, 1150, 1603, 1804, 2067
BstH2I RGCGCY 1 cut(s) 757
BstHHI GCGC 4 cut(s) 266, 756, 1258, 1341
BstKTI GATC 7 cut(s) 408, 704, 1215, 1327, 1738, 2217, 2323
BstMAI GTCTC 3 cut(s) 380, 1618, 1992
BstMBI GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
BstMWI GCNNNNNNNGC 4 cut(s) 47, 239, 753, 2336
BstNI CCWGG 1 cut(s) 1880
BstNSI RCATGY 1 cut(s) 1448
BstSCI CCNGG 2 cut(s) 70, 1878
BstSFI CTRYAG 1 cut(s) 1090
BstSLI GKGCMC 2 cut(s) 179, 1303
BstV1I GCAGC 3 cut(s) 25, 956, 2103
BstX2I RGATCY 1 cut(s) 701
BstXI CCANNNNNNTGG 2 cut(s) 296, 866
BstYI RGATCY 1 cut(s) 701
BsuI GTATCC 3 cut(s) 448, 527, 1040
BsuRI GGCC 5 cut(s) 613, 747, 1490, 2169, 2335
BtgI CCRYGG 2 cut(s) 1491, 1987
BtrI CACGTC 2 cut(s) 1422, 2095
BtsCI GGATG 7 cut(s) 243, 564, 907, 1150, 1603, 1804, 2067
BtsI GCAGTG 1 cut(s) 1842
BtsIMutI CAGTG 6 cut(s) 51, 177, 1139, 1163, 1842, 1894
Cac8I GCNNGC 6 cut(s) 364, 856, 974, 1090, 1446, 2337
CciI TCATGA 1 cut(s) 1450
CfoI GCGC 4 cut(s) 266, 756, 1258, 1341
Cfr10I RCCGGY 2 cut(s) 1472, 1535
Cfr13I GGNCC 4 cut(s) 273, 587, 746, 1773
CseI GACGC 1 cut(s) 1981
Csp6I GTAC 9 cut(s) 519, 793, 918, 1063, 1354, 1889, 2098, 2195, 2312
CspAI ACCGGT 1 cut(s) 1535
CviQI GTAC 9 cut(s) 519, 793, 918, 1063, 1354, 1889, 2098, 2195, 2312
DdeI CTNAG 2 cut(s) 1244, 2129
DpnI GATC 7 cut(s) 407, 703, 1214, 1326, 1737, 2216, 2322
DpnII GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
EaeI YGGCCR 3 cut(s) 611, 1488, 2167
Eam1104I CTCTTC 5 cut(s) 1179, 1439, 1449, 1600, 1616
EarI CTCTTC 5 cut(s) 1179, 1439, 1449, 1600, 1616
EciI GGCGGA 2 cut(s) 205, 2105
Eco130I CCWWGG 4 cut(s) 514, 1491, 1776, 2085
Eco147I AGGCCT 1 cut(s) 2335
Eco24I GRGCYC 2 cut(s) 258, 1250
Eco32I GATATC 2 cut(s) 1190, 1984
Eco47I GGWCC 3 cut(s) 273, 587, 1773
Eco57I CTGAAG 3 cut(s) 936, 1350, 1784
Eco88I CYCGRG 1 cut(s) 1944
EcoO109I RGGNCCY 1 cut(s) 1773
EcoRI GAATTC 1 cut(s) 1096
EcoRII CCWGG 1 cut(s) 1878
EcoRV GATATC 2 cut(s) 1190, 1984
EcoT14I CCWWGG 4 cut(s) 514, 1491, 1776, 2085
EcoT38I GRGCYC 2 cut(s) 258, 1250
ErhI CCWWGG 4 cut(s) 514, 1491, 1776, 2085
FalI AAGNNNNNCTT 6 cut(s) 637, 669, 2128, 2160, 2214, 2246
FaqI GGGAC 1 cut(s) 1072
FauNDI CATATG 1 cut(s) 428
FbaI TGATCA 3 cut(s) 405, 1212, 1735
FblI GTMKAC 1 cut(s) 992
Fnu4HI GCNGC 3 cut(s) 39, 970, 2117
FokI GGATG 7 cut(s) 250, 551, 894, 1157, 1610, 1811, 2074
FriOI GRGCYC 2 cut(s) 258, 1250
Fsp4HI GCNGC 3 cut(s) 39, 970, 2117
FspBI CTAG 5 cut(s) 666, 963, 1628, 1838, 2148
GlaI GCGC 4 cut(s) 265, 755, 1257, 1340
GluI GCNGC 3 cut(s) 39, 970, 2117
GsuI CTGGAG 1 cut(s) 2037
HaeII RGCGCY 1 cut(s) 757
HaeIII GGCC 5 cut(s) 613, 747, 1490, 2169, 2335
HapII CCGG 3 cut(s) 71, 1473, 1536
HgaI GACGC 1 cut(s) 1981
HhaI GCGC 4 cut(s) 266, 756, 1258, 1341
Hin1I GRCGYC 1 cut(s) 1992
Hin6I GCGC 4 cut(s) 264, 754, 1256, 1339
HinP1I GCGC 4 cut(s) 264, 754, 1256, 1339
HincII GTYRAC 2 cut(s) 59, 342
HindII GTYRAC 2 cut(s) 59, 342
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 55, 806, 998
HpaII CCGG 3 cut(s) 71, 1473, 1536
HphI GGTGA 7 cut(s) 129, 890, 928, 1501, 1630, 1681, 2215
Hpy166II GTNNAC 9 cut(s) 59, 207, 342, 607, 993, 1301, 1690, 1753, 2098
Hpy8I GTNNAC 9 cut(s) 59, 207, 342, 607, 993, 1301, 1690, 1753, 2098
Hpy99I CGWCG 1 cut(s) 454
HpyAV CCTTC 5 cut(s) 109, 960, 987, 1625, 2219
HpyCH4IV ACGT 3 cut(s) 1061, 1421, 2094
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 239, 753, 2336
HpyF3I CTNAG 2 cut(s) 1244, 2129
HpySE526I ACGT 3 cut(s) 1061, 1421, 2094
Hsp92I GRCGYC 1 cut(s) 1992
HspAI GCGC 4 cut(s) 264, 754, 1256, 1339
Ksp22I TGATCA 3 cut(s) 405, 1212, 1735
Kzo9I GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
LmnI GCTCC 5 cut(s) 104, 851, 1263, 2056, 2356
Lsp1109I GCAGC 3 cut(s) 25, 956, 2103
LweI GCATC 6 cut(s) 172, 613, 737, 1109, 1644, 2071
MaeI CTAG 5 cut(s) 666, 963, 1628, 1838, 2148
MaeII ACGT 3 cut(s) 1061, 1421, 2094
MaeIII GTNAC 4 cut(s) 1160, 1475, 1538, 2203
MalI GATC 7 cut(s) 407, 703, 1214, 1326, 1737, 2216, 2322
MboI GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 4 cut(s) 179, 258, 1250, 1303
MlsI TGGCCA 2 cut(s) 1490, 2169
MluNI TGGCCA 2 cut(s) 1490, 2169
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 2 cut(s) 473, 829
Mox20I TGGCCA 2 cut(s) 1490, 2169
MroXI GAANNNNTTC 1 cut(s) 2143
MscI TGGCCA 2 cut(s) 1490, 2169
MseI TTAA 5 cut(s) 86, 104, 761, 1182, 1224
MslI CAYNNNNRTG 3 cut(s) 294, 1449, 1899
Msp20I TGGCCA 2 cut(s) 1490, 2169
MspCI CTTAAG 1 cut(s) 85
MspI CCGG 3 cut(s) 71, 1473, 1536
MspR9I CCNGG 2 cut(s) 72, 1880
Mva1269I GAATGC 1 cut(s) 1114
MvaI CCWGG 1 cut(s) 1880
MwoI GCNNNNNNNGC 4 cut(s) 47, 239, 753, 2336
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 1 cut(s) 1491
NdeI CATATG 1 cut(s) 428
NdeII GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
NlaIV GGNNCC 5 cut(s) 274, 1230, 1774, 1941, 2035
NmeAIII GCCGAG 6 cut(s) 355, 639, 793, 864, 1753, 2230
NmuCI GTSAC 1 cut(s) 2203
NspI RCATGY 1 cut(s) 1448
PaeI GCATGC 1 cut(s) 1448
PagI TCATGA 1 cut(s) 1450
PceI AGGCCT 1 cut(s) 2335
PctI GAATGC 1 cut(s) 1114
PdmI GAANNNNTTC 1 cut(s) 2143
PfeI GAWTC 2 cut(s) 806, 998
PinAI ACCGGT 1 cut(s) 1535
PkrI GCNGC 3 cut(s) 40, 971, 2118
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PpuMI RGGWCCY 1 cut(s) 1773
PsiI TTATAA 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 1773
Psp6I CCWGG 1 cut(s) 1878
PspGI CCWGG 1 cut(s) 1878
PspN4I GGNNCC 5 cut(s) 274, 1230, 1774, 1941, 2035
PspPI GGNCC 4 cut(s) 273, 587, 746, 1773
PspPPI RGGWCCY 1 cut(s) 1773
PstI CTGCAG 1 cut(s) 1094
PsuI RGATCY 1 cut(s) 701
RsaI GTAC 9 cut(s) 520, 794, 919, 1064, 1355, 1890, 2099, 2196, 2313
RsaNI GTAC 9 cut(s) 519, 793, 918, 1063, 1354, 1889, 2098, 2195, 2312
RseI CAYNNNNRTG 3 cut(s) 294, 1449, 1899
SaqAI TTAA 5 cut(s) 86, 104, 761, 1182, 1224
SatI GCNGC 3 cut(s) 39, 970, 2117
Sau3AI GATC 7 cut(s) 405, 701, 1212, 1324, 1735, 2214, 2320
Sau96I GGNCC 4 cut(s) 273, 587, 746, 1773
SbfI CCTGCAGG 1 cut(s) 1094
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 2 cut(s) 72, 1880
SdaI CCTGCAGG 1 cut(s) 1094
SduI GDGCHC 4 cut(s) 179, 258, 1250, 1303
SfaNI GCATC 6 cut(s) 172, 613, 737, 1109, 1644, 2071
SfcI CTRYAG 1 cut(s) 1090
SinI GGWCC 3 cut(s) 273, 587, 1773
SmiMI CAYNNNNRTG 3 cut(s) 294, 1449, 1899
SmlI CTYRAG 4 cut(s) 85, 154, 383, 2231
SmoI CTYRAG 4 cut(s) 85, 154, 383, 2231
SphI GCATGC 1 cut(s) 1448
Sse8387I CCTGCAGG 1 cut(s) 1094
SseBI AGGCCT 1 cut(s) 2335
SsiI CCGC 3 cut(s) 216, 1616, 2090
SspMI CTAG 5 cut(s) 666, 963, 1628, 1838, 2148
StuI AGGCCT 1 cut(s) 2335
StyD4I CCNGG 2 cut(s) 70, 1878
StyI CCWWGG 4 cut(s) 514, 1491, 1776, 2085
TaiI ACGT 3 cut(s) 1064, 1424, 2097
TaqI TCGA 2 cut(s) 599, 2244
TatI WGTACW 3 cut(s) 792, 917, 2097
TfiI GAWTC 2 cut(s) 806, 998
Tru1I TTAA 5 cut(s) 86, 104, 761, 1182, 1224
Tru9I TTAA 5 cut(s) 86, 104, 761, 1182, 1224
TscAI CASTG 6 cut(s) 51, 184, 1146, 1163, 1849, 1901
TseFI GTSAC 1 cut(s) 2203
TseI GCWGC 3 cut(s) 38, 969, 2116
Tsp45I GTSAC 1 cut(s) 2203
TspDTI ATGAA 9 cut(s) 819, 1006, 1068, 1089, 1373, 1467, 2081, 2193, 2306
TspGWI ACGGA 1 cut(s) 467
TspRI CASTG 6 cut(s) 51, 184, 1146, 1163, 1849, 1901
Vha464I CTTAAG 1 cut(s) 85
VneI GTGCAC 1 cut(s) 1299
VpaK11BI GGWCC 3 cut(s) 273, 587, 1773
XapI RAATTY 4 cut(s) 548, 718, 1096, 1805
XbaI TCTAGA 2 cut(s) 665, 962
XceI RCATGY 1 cut(s) 1448
XmiI GTMKAC 1 cut(s) 992
XmnI GAANNNNTTC 1 cut(s) 2143
XspI CTAG 5 cut(s) 666, 963, 1628, 1838, 2148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.