MD12G1151200.v1.1

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000166
Physical Location & Seq
Forward (+)
23114611 .. 23116995
2385 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1151200.v1.1.491

Sequence Viewer

Length: 2385 bp
ATGGAAGTTCTCAAACTTCTTGTTCTTTCGCTGTTTGCAGCCACGGTTGGTTGGAGTCAAGCGGGGTTCACAGGGCTGCAAAGCCTAAGGCCGGGGAGTTCTTTGATAGTTGAGGAAGAAGGTGACTTCTTGGTTTCGCCCAACGGAACTTTCTCCGCTGGATTTTACCGCATTGGCAGCAATTCATCTTGTTATTCAATATGGTTCACAAAGTCAGCCGATAAAACAGTGGTTTGGATGGCGAATAGAGACAGACCGGTCAGCGTACGAGGATCCAAGCTAACCTTCCACAGAAATGGCAACCTGGTCTTGACTGATGGGGTTGGATCGATTATATGGTCAACCAACACTATCTCCAACACGGGCTTGGAAGCACGGCTTCTTGAGACCGGAAACTTGGTGCTGCTCAACCAAGAAAAGAAGGTCATCTGGCAGAGTTTTGATTTTCCCACGGATACACTTCTACCGTCACAGAAATTTGTCAAGAACACAACCTTGGTGTCTATGAGAAGTCAAGGTACATACTTATCTGGATTTTACAACTTCAAATTTGACGATAATAATGTACTATATCTCATATACAATGGCGCTCTACTTTCAAGTGTTTATTGGCCAAGGACGGACCTAACTGTTTTCCAGAGTGGTAGAACACCTTACAATAGTTCTAGACTAGCAATTCTGGATGAGGCCGGACAATTTATATCGAGTGACAATTTGAAGTTCAATGCATCTGATTACGGGGTTGGTCCAAAACGCCGTTTAACAATGGATTATGATGGCATCTTGAGGTTGTACAGTCTAAATGAATCATCCGGGCTTTGGGAACTGTCATGGTTGCCTGAGGGTGTCGATGCGTGCTTGGTTCACGGCTTGTGTGGTGAATACGGTGTTTGCACTTACAAACCACAACCTACTTGTGCTTGCCCTCATGGTTTCACCAGAAACGATCCTTCAGATGGGTCGAAAGGCTGCTCGCCAGTGTTCAATTTGAGCCATGATTCAAACAATTCAGAATTCATGGAGCTTCCCAACACAGATTACTATGGATATGACTTGGAAACTTATTCTCCCAAAGTTTCTTTTCAAGAATGCCAGAAATCGTGCCAAAACGATACAAGATGTAAAGGGTTCGGATATACTCTGGATGGACTTGGACATTGTTTCCCTAAAAGTTTACTCCTTAACGGATTAAGTATGCCAAGCACTCTCCAAATCATGCATATCAAAATTCCGAAGGGCTTTCTGAGCAATGAAGTCCTAAACGGGCTAGTAACATATGATTTGAACTGTTCAGCGGCACAAGTTGTTCTCCAAAGTAGTGATGTTGAAGTGGAAAAGGGAAATAAAAATCGGTACATGGATTACCTGATTGGTTTTGTGAGCTCATTTGCGGTAATTGAAGCAATCTGCATTGGCTTGACATGGTGGTACATCTTCCGAAAGCAGGCTAAAGAGGAATTTGTGAACATGGGTTACATAGCATTAGCCATGGGATTCAAGCGCTTCACGTACGCAGAACTAAAAAAAGCAACCAATAATTTCAAGCAGGAGGTAGGAAGAGGAGGCTTTGGAACCGTGTATAAAGGGGTCCTAGACGACGAAAGAGTTGTAGCTGTGAAGAGACTAGATGGTGTCTTGCAAGGAGATGCAGAGTTTTGGGCAGAGGTGAGTGTGATAGGGAATATCAATCACCGAAACTTGGTTAAACTGTGGGGTTTCTGCACGGACAATCAGCATAAGCTTCTCGTCTATGAGTACCTTGAGAATGGATCATTGGACAAAATCTTGTTCTCAGATGTGGAACTAGGATTGGAGCAGAGATACAAGATTGCGGTAGGCACTGCAAAGGGTTTGTCGTATTTACATGAGGAGTGCCTTGAGTGGGTGCTCCACTGCGATGTAAAGCCTCAAAACATACTGTTAGATGATCAGCTTGAACCTAAAGTGGCAGACTTTGGGATGTCAAAGCTATTCACAGATATCCATGGCATCAAAGACATGCAAGGGATGGGATTTTCAAAAGCGCGGGGCACCAGAGGTTATATGGCTCCGGAGTGGATGATGAACCTGAACATTGATGCCAAGGCGGATGTGTACAGCTATGGGATTGTTTTGCTGGAACTTCTGAGTGGAAAGAGCGCTTCGATTCTCATTTCAGCGCTTGCCAAAGAGTACAATGAGTGCAACCAACTGGTTCAATTTGTGACTGAAAAGATAAAAAAAGAAGGGCTTAAGCAAGTGATTGACCAGAGACTACTGCCTGAATTCGAATCGAACAAGCTCGAAAGATTGATGAAAGTTGCTCTGTTGTGTGTTCACGAGGACCGCAATGCAAGGCCAGCCATGAGTAAGGTTGTCGAGATTCTGCTTGAAAATATTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

795

Amino Acids

89.02

Weight (kDa)

5.73

Isoelectric Point (pI)

36.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 165 6.6e-26 D-mannose binding lectin
S_locus_glycop PF00954 252 - 314 9.3e-09 S-locus glycoprotein domain
PAN_4 PF14295 345 - 382 9.2e-06 PAN domain
Pkinase PF00069 514 - 786 5.2e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 514 - 789 1.6e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 2030
AccII CGCG 1 cut(s) 2026
AccIII TCCGGA 1 cut(s) 2050
AciI CCGC 9 cut(s) 62, 156, 169, 1295, 1391, 1832, 2026, 2087, 2326
AclWI GGATC 5 cut(s) 267, 280, 334, 941, 1777
AcoI YGGCCR 1 cut(s) 611
AcsI RAATTY 6 cut(s) 476, 548, 1013, 1227, 1457, 2264
AcuI CTGAAG 1 cut(s) 936
AfeI AGCGCT 3 cut(s) 1502, 2140, 2160
AfiI CCNNNNNNNGG 6 cut(s) 91, 819, 956, 1444, 1699, 1882
AflII CTTAAG 1 cut(s) 2231
AgeI ACCGGT 1 cut(s) 256
AjnI CCWGG 1 cut(s) 303
AjuI GAANNNNNNNTTGG 8 cut(s) 134, 166, 269, 301, 479, 511, 1757, 1789
AluBI AGCT 9 cut(s) 280, 1024, 1383, 1613, 1741, 1933, 1969, 2100, 2281
AluI AGCT 9 cut(s) 280, 1024, 1383, 1613, 1741, 1933, 1969, 2100, 2281
Alw21I GWGCWC 2 cut(s) 1385, 1890
Alw26I GTCTC 4 cut(s) 243, 380, 1615, 2245
AlwI GGATC 5 cut(s) 267, 280, 334, 941, 1777
Aor13HI TCCGGA 1 cut(s) 2050
Aor51HI AGCGCT 3 cut(s) 1502, 2140, 2160
AoxI GGCC 4 cut(s) 89, 611, 687, 2336
ApeKI GCWGC 5 cut(s) 38, 76, 177, 403, 969
ApoI RAATTY 6 cut(s) 476, 548, 1013, 1227, 1457, 2264
ArsI GACNNNNNNTTYG 2 cut(s) 1838, 1870
AsiGI ACCGGT 1 cut(s) 256
AspLEI GCGC 5 cut(s) 590, 1503, 2026, 2141, 2161
AspS9I GGNCC 4 cut(s) 622, 746, 1588, 2323
AsuC2I CCSGG 2 cut(s) 93, 814
AsuHPI GGTGA 5 cut(s) 134, 890, 928, 1678, 1682
AsuII TTCGAA 1 cut(s) 2268
AvaII GGWCC 4 cut(s) 622, 746, 1588, 2323
AxyI CCTNAGG 2 cut(s) 86, 840
BaeGI GKGCMC 1 cut(s) 2033
BalI TGGCCA 1 cut(s) 613
BamHI GGATCC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 2030
BanII GRGCYC 1 cut(s) 1385
BarI GAAGNNNNNNTAC 2 cut(s) 502, 534
BauI CACGAG 1 cut(s) 2318
Bbv12I GWGCWC 2 cut(s) 1385, 1890
BbvI GCAGC 5 cut(s) 50, 63, 189, 390, 956
BccI CCATC 7 cut(s) 232, 311, 770, 950, 1139, 1622, 2002
BceAI ACGGC 3 cut(s) 392, 741, 883
BciT130I CCWGG 1 cut(s) 305
BciVI GTATCC 1 cut(s) 448
BclI TGATCA 1 cut(s) 1927
BcnI CCSGG 2 cut(s) 93, 814
BcoDI GTCTC 4 cut(s) 243, 380, 1615, 2245
BfaI CTAG 6 cut(s) 666, 671, 1268, 1592, 1625, 1805
BfoI RGCGCY 4 cut(s) 591, 1504, 2142, 2162
BfrI CTTAAG 1 cut(s) 2231
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 6 cut(s) 39, 77, 178, 404, 970, 1296
BlsI GCNGC 6 cut(s) 40, 78, 179, 405, 971, 1297
Bme1390I CCNGG 3 cut(s) 93, 305, 814
Bme18I GGWCC 4 cut(s) 622, 746, 1588, 2323
BmgT120I GGNCC 4 cut(s) 622, 746, 1588, 2323
BmiI GGNNCC 5 cut(s) 274, 1573, 1589, 2032, 2049
BmrFI CCNGG 3 cut(s) 93, 305, 814
BmsI GCATC 6 cut(s) 737, 789, 841, 1636, 1998, 2068
Bpu14I TTCGAA 1 cut(s) 2268
BpuEI CTTGAG 4 cut(s) 404, 805, 1781, 1898
BpuMI CCSGG 2 cut(s) 93, 814
Bsa29I ATCGAT 1 cut(s) 329
BsaAI YACGTR 1 cut(s) 1509
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 8 cut(s) 42, 92, 450, 495, 614, 1488, 1984, 2082
BsaWI WCCGGW 3 cut(s) 256, 389, 2050
BsaXI ACNNNNNCTCC 4 cut(s) 338, 368, 1051, 1081
Bsc4I CCNNNNNNNGG 6 cut(s) 91, 819, 956, 1444, 1699, 1882
Bse118I RCCGGY 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 977, 2196
Bse21I CCTNAGG 2 cut(s) 86, 840
Bse3DI GCAATG 2 cut(s) 1255, 2335
BseAI TCCGGA 1 cut(s) 2050
BseBI CCWGG 1 cut(s) 305
BseCI ATCGAT 1 cut(s) 329
BseDI CCNNGG 8 cut(s) 42, 92, 450, 495, 614, 1488, 1984, 2082
BseGI GGATG 8 cut(s) 243, 688, 809, 1150, 1965, 2013, 2064, 2095
BseLI CCNNNNNNNGG 6 cut(s) 91, 819, 956, 1444, 1699, 1882
BseMI GCAATG 2 cut(s) 1255, 2335
BseMII CTCAG 4 cut(s) 831, 1235, 1806, 2117
BseNI ACTGG 2 cut(s) 977, 2196
BseRI GAGGAG 2 cut(s) 1575, 1883
BseSI GKGCMC 1 cut(s) 2033
BseXI GCAGC 5 cut(s) 50, 63, 189, 390, 956
BsgI GTGCAG 1 cut(s) 1705
Bsh1236I CGCG 1 cut(s) 2026
BshFI GGCC 4 cut(s) 91, 613, 689, 2338
BshNI GGYRCC 1 cut(s) 2030
BshTI ACCGGT 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 329
BsiHKAI GWGCWC 2 cut(s) 1385, 1890
BsiSI CCGG 6 cut(s) 92, 257, 390, 690, 813, 2051
BsiWI CGTACG 2 cut(s) 265, 1509
BslI CCNNNNNNNGG 6 cut(s) 91, 819, 956, 1444, 1699, 1882
BsmAI GTCTC 4 cut(s) 243, 380, 1615, 2245
BsmI GAATGC 1 cut(s) 1094
BsnI GGCC 4 cut(s) 91, 613, 689, 2338
Bso31I GGTCTC 1 cut(s) 380
Bsp119I TTCGAA 1 cut(s) 2268
Bsp1286I GDGCHC 3 cut(s) 1385, 1890, 2033
Bsp13I TCCGGA 1 cut(s) 2050
Bsp1407I TGTACA 2 cut(s) 792, 2094
Bsp143I GATC 5 cut(s) 272, 326, 946, 1769, 1927
Bsp19I CCATGG 2 cut(s) 1488, 1984
BspACI CCGC 9 cut(s) 62, 156, 169, 1295, 1391, 1832, 2026, 2087, 2326
BspANI GGCC 4 cut(s) 91, 613, 689, 2338
BspCNI CTCAG 4 cut(s) 832, 1236, 1805, 2118
BspDI ATCGAT 1 cut(s) 329
BspEI TCCGGA 1 cut(s) 2050
BspFNI CGCG 1 cut(s) 2026
BspLI GGNNCC 5 cut(s) 274, 1573, 1589, 2032, 2049
BspPI GGATC 5 cut(s) 267, 280, 334, 941, 1777
BspT104I TTCGAA 1 cut(s) 2268
BspT107I GGYRCC 1 cut(s) 2030
BspTI CTTAAG 1 cut(s) 2231
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 2 cut(s) 1255, 2335
BsrFI RCCGGY 1 cut(s) 256
BsrGI TGTACA 2 cut(s) 792, 2094
BsrI ACTGG 2 cut(s) 977, 2196
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 8 cut(s) 42, 92, 450, 495, 614, 1488, 1984, 2082
BssMI GATC 5 cut(s) 272, 326, 946, 1769, 1927
BssSI CACGAG 1 cut(s) 2318
BssT1I CCWWGG 5 cut(s) 495, 614, 1488, 1984, 2082
Bst2BI CACGAG 1 cut(s) 2318
Bst2UI CCWGG 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 1552, 1613
BstAFI CTTAAG 1 cut(s) 2231
BstAUI TGTACA 2 cut(s) 792, 2094
BstBAI YACGTR 1 cut(s) 1509
BstBI TTCGAA 1 cut(s) 2268
BstC8I GCNNGC 6 cut(s) 856, 922, 974, 1446, 2163, 2340
BstDEI CTNAG 5 cut(s) 86, 840, 1244, 1792, 2126
BstDSI CCRYGG 4 cut(s) 42, 450, 1488, 1984
BstF5I GGATG 8 cut(s) 243, 688, 809, 1150, 1965, 2013, 2064, 2095
BstFNI CGCG 1 cut(s) 2026
BstH2I RGCGCY 4 cut(s) 591, 1504, 2142, 2162
BstHHI GCGC 5 cut(s) 590, 1503, 2026, 2141, 2161
BstKTI GATC 5 cut(s) 275, 329, 949, 1772, 1930
BstMAI GTCTC 4 cut(s) 243, 380, 1615, 2245
BstMBI GATC 5 cut(s) 272, 326, 946, 1769, 1927
BstMWI GCNNNNNNNGC 3 cut(s) 177, 1245, 2339
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 2002
BstSCI CCNGG 3 cut(s) 91, 303, 812
BstSLI GKGCMC 1 cut(s) 2033
BstUI CGCG 1 cut(s) 2026
BstV1I GCAGC 5 cut(s) 50, 63, 189, 390, 956
BstX2I RGATCY 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 1 cut(s) 272
Bsu15I ATCGAT 1 cut(s) 329
Bsu36I CCTNAGG 2 cut(s) 86, 840
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 4 cut(s) 91, 613, 689, 2338
BsuTUI ATCGAT 1 cut(s) 329
BtgI CCRYGG 4 cut(s) 42, 450, 1488, 1984
BtgZI GCGATG 1 cut(s) 1911
BtsCI GGATG 8 cut(s) 243, 688, 809, 1150, 1965, 2013, 2064, 2095
BtsI GCAGTG 2 cut(s) 1839, 1891
BtsIMutI CAGTG 4 cut(s) 234, 984, 1839, 1891
Cac8I GCNNGC 6 cut(s) 856, 922, 974, 1446, 2163, 2340
CfoI GCGC 5 cut(s) 590, 1503, 2026, 2141, 2161
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 4 cut(s) 622, 746, 1588, 2323
ClaI ATCGAT 1 cut(s) 329
CsiI ACCWGGT 1 cut(s) 303
CspAI ACCGGT 1 cut(s) 256
DdeI CTNAG 5 cut(s) 86, 840, 1244, 1792, 2126
DpnI GATC 5 cut(s) 274, 328, 948, 1771, 1929
DpnII GATC 5 cut(s) 272, 326, 946, 1769, 1927
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 1 cut(s) 611
Eam1104I CTCTTC 2 cut(s) 1552, 1613
EarI CTCTTC 2 cut(s) 1552, 1613
EciI GGCGGA 1 cut(s) 2102
Ecl136II GAGCTC 1 cut(s) 1383
Eco130I CCWWGG 5 cut(s) 495, 614, 1488, 1984, 2082
Eco24I GRGCYC 1 cut(s) 1385
Eco31I GGTCTC 1 cut(s) 380
Eco32I GATATC 1 cut(s) 1981
Eco47I GGWCC 4 cut(s) 622, 746, 1588, 2323
Eco47III AGCGCT 3 cut(s) 1502, 2140, 2160
Eco53kI GAGCTC 1 cut(s) 1383
Eco57I CTGAAG 1 cut(s) 936
Eco81I CCTNAGG 2 cut(s) 86, 840
EcoICRI GAGCTC 1 cut(s) 1383
EcoO109I RGGNCCY 1 cut(s) 1588
EcoRI GAATTC 2 cut(s) 1013, 2264
EcoRII CCWGG 1 cut(s) 303
EcoRV GATATC 1 cut(s) 1981
EcoT14I CCWWGG 5 cut(s) 495, 614, 1488, 1984, 2082
EcoT22I ATGCAT 2 cut(s) 730, 1221
EcoT38I GRGCYC 1 cut(s) 1385
ErhI CCWWGG 5 cut(s) 495, 614, 1488, 1984, 2082
FalI AAGNNNNNCTT 6 cut(s) 269, 301, 363, 395, 2214, 2246
FauI CCCGC 2 cut(s) 55, 2019
FauNDI CATATG 1 cut(s) 1276
FbaI TGATCA 1 cut(s) 1927
Fnu4HI GCNGC 6 cut(s) 39, 77, 178, 404, 970, 1296
FokI GGATG 8 cut(s) 250, 695, 796, 1157, 1972, 2020, 2071, 2102
FriOI GRGCYC 1 cut(s) 1385
Fsp4HI GCNGC 6 cut(s) 39, 77, 178, 404, 970, 1296
FspBI CTAG 6 cut(s) 666, 671, 1268, 1592, 1625, 1805
GlaI GCGC 5 cut(s) 589, 1502, 2025, 2140, 2160
GluI GCNGC 6 cut(s) 39, 77, 178, 404, 970, 1296
HaeII RGCGCY 4 cut(s) 591, 1504, 2142, 2162
HaeIII GGCC 4 cut(s) 91, 613, 689, 2338
HapII CCGG 6 cut(s) 92, 257, 390, 690, 813, 2051
HhaI GCGC 5 cut(s) 590, 1503, 2026, 2141, 2161
Hin6I GCGC 5 cut(s) 588, 1501, 2024, 2139, 2159
HinP1I GCGC 5 cut(s) 588, 1501, 2024, 2139, 2159
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 1739
HinfI GANTC 7 cut(s) 55, 806, 998, 1494, 2146, 2270, 2362
HpaII CCGG 6 cut(s) 92, 257, 390, 690, 813, 2051
HphI GGTGA 5 cut(s) 134, 890, 928, 1678, 1682
Hpy166II GTNNAC 8 cut(s) 69, 207, 342, 865, 1175, 1465, 2095, 2317
Hpy188I TCNGA 9 cut(s) 733, 955, 1012, 1133, 1233, 1245, 1439, 1795, 2127
Hpy8I GTNNAC 8 cut(s) 69, 207, 342, 865, 1175, 1465, 2095, 2317
Hpy99I CGWCG 1 cut(s) 1601
HpyAV CCTTC 6 cut(s) 113, 295, 415, 960, 1228, 2219
HpyCH4IV ACGT 1 cut(s) 1508
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 1245, 2339
HpyF3I CTNAG 5 cut(s) 86, 840, 1244, 1792, 2126
HpySE526I ACGT 1 cut(s) 1508
HspAI GCGC 5 cut(s) 588, 1501, 2024, 2139, 2159
Kpn2I TCCGGA 1 cut(s) 2050
Ksp22I TGATCA 1 cut(s) 1927
Kzo9I GATC 5 cut(s) 272, 326, 946, 1769, 1927
LmnI GCTCC 4 cut(s) 1021, 1813, 1893, 2053
Lsp1109I GCAGC 5 cut(s) 50, 63, 189, 390, 956
LweI GCATC 6 cut(s) 737, 789, 841, 1636, 1998, 2068
MabI ACCWGGT 1 cut(s) 303
MaeI CTAG 6 cut(s) 666, 671, 1268, 1592, 1625, 1805
MaeII ACGT 1 cut(s) 1508
MaeIII GTNAC 6 cut(s) 122, 468, 707, 1270, 1472, 2203
MalI GATC 5 cut(s) 274, 328, 948, 1771, 1929
MboI GATC 5 cut(s) 272, 326, 946, 1769, 1927
MboII GAAGA 4 cut(s) 128, 1426, 1569, 1630
MflI RGATCY 1 cut(s) 272
MhlI GDGCHC 3 cut(s) 1385, 1890, 2033
MlsI TGGCCA 1 cut(s) 613
MluNI TGGCCA 1 cut(s) 613
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 3 cut(s) 32, 304, 381
Mox20I TGGCCA 1 cut(s) 613
Mph1103I ATGCAT 2 cut(s) 730, 1221
MroI TCCGGA 1 cut(s) 2050
MscI TGGCCA 1 cut(s) 613
MseI TTAA 5 cut(s) 761, 1182, 1190, 1704, 2232
MslI CAYNNNNRTG 2 cut(s) 294, 1896
Msp20I TGGCCA 1 cut(s) 613
MspA1I CMGCKG 2 cut(s) 158, 1295
MspCI CTTAAG 1 cut(s) 2231
MspI CCGG 6 cut(s) 92, 257, 390, 690, 813, 2051
MspR9I CCNGG 3 cut(s) 93, 305, 814
Mva1269I GAATGC 1 cut(s) 1094
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 1 cut(s) 2026
MwoI GCNNNNNNNGC 3 cut(s) 177, 1245, 2339
NciI CCSGG 2 cut(s) 93, 814
NcoI CCATGG 2 cut(s) 1488, 1984
NdeI CATATG 1 cut(s) 1276
NdeII GATC 5 cut(s) 272, 326, 946, 1769, 1927
NlaIV GGNNCC 5 cut(s) 274, 1573, 1589, 2032, 2049
NmuCI GTSAC 4 cut(s) 122, 468, 707, 2203
NsiI ATGCAT 2 cut(s) 730, 1221
NspI RCATGY 1 cut(s) 2002
NspV TTCGAA 1 cut(s) 2268
PctI GAATGC 1 cut(s) 1094
PfeI GAWTC 6 cut(s) 806, 998, 1494, 2146, 2270, 2362
Pfl23II CGTACG 2 cut(s) 265, 1509
PinAI ACCGGT 1 cut(s) 256
PkrI GCNGC 6 cut(s) 40, 78, 179, 405, 971, 1297
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
Ppu21I YACGTR 1 cut(s) 1509
PpuMI RGGWCCY 1 cut(s) 1588
Psp124BI GAGCTC 1 cut(s) 1385
Psp5II RGGWCCY 1 cut(s) 1588
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspLI CGTACG 2 cut(s) 265, 1509
PspN4I GGNNCC 5 cut(s) 274, 1573, 1589, 2032, 2049
PspPI GGNCC 4 cut(s) 622, 746, 1588, 2323
PspPPI RGGWCCY 1 cut(s) 1588
PsrI GAACNNNNNNTAC 2 cut(s) 1457, 1489
PsuI RGATCY 1 cut(s) 272
RseI CAYNNNNRTG 2 cut(s) 294, 1896
SacI GAGCTC 1 cut(s) 1385
SaqAI TTAA 5 cut(s) 761, 1182, 1190, 1704, 2232
SatI GCNGC 6 cut(s) 39, 77, 178, 404, 970, 1296
Sau3AI GATC 5 cut(s) 272, 326, 946, 1769, 1927
Sau96I GGNCC 4 cut(s) 622, 746, 1588, 2323
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 3 cut(s) 93, 305, 814
SduI GDGCHC 3 cut(s) 1385, 1890, 2033
SexAI ACCWGGT 1 cut(s) 303
SfaNI GCATC 6 cut(s) 737, 789, 841, 1636, 1998, 2068
SfuI TTCGAA 1 cut(s) 2268
SinI GGWCC 4 cut(s) 622, 746, 1588, 2323
SmiMI CAYNNNNRTG 2 cut(s) 294, 1896
SmlI CTYRAG 5 cut(s) 383, 784, 1760, 1877, 2231
SmoI CTYRAG 5 cut(s) 383, 784, 1760, 1877, 2231
SsiI CCGC 9 cut(s) 62, 156, 169, 1295, 1391, 1832, 2026, 2087, 2326
SspI AATATT 1 cut(s) 2377
SspMI CTAG 6 cut(s) 666, 671, 1268, 1592, 1625, 1805
SstI GAGCTC 1 cut(s) 1385
StyD4I CCNGG 3 cut(s) 91, 303, 812
StyI CCWWGG 5 cut(s) 495, 614, 1488, 1984, 2082
TaiI ACGT 1 cut(s) 1511
TaqI TCGA 9 cut(s) 329, 704, 849, 962, 2144, 2268, 2273, 2283, 2358
TatI WGTACW 4 cut(s) 565, 792, 2094, 2172
TauI GCSGC 1 cut(s) 1298
TfiI GAWTC 6 cut(s) 806, 998, 1494, 2146, 2270, 2362
Tru1I TTAA 5 cut(s) 761, 1182, 1190, 1704, 2232
Tru9I TTAA 5 cut(s) 761, 1182, 1190, 1704, 2232
TscAI CASTG 4 cut(s) 234, 984, 1846, 1898
TseFI GTSAC 4 cut(s) 122, 468, 707, 2203
TseI GCWGC 5 cut(s) 38, 76, 177, 403, 969
Tsp45I GTSAC 4 cut(s) 122, 468, 707, 2203
TspDTI ATGAA 6 cut(s) 174, 819, 1006, 1266, 2078, 2309
TspGWI ACGGA 5 cut(s) 159, 467, 635, 1200, 1739
TspRI CASTG 4 cut(s) 234, 984, 1846, 1898
Vha464I CTTAAG 1 cut(s) 2231
VpaK11BI GGWCC 4 cut(s) 622, 746, 1588, 2323
XapI RAATTY 6 cut(s) 476, 548, 1013, 1227, 1457, 2264
XbaI TCTAGA 1 cut(s) 665
XceI RCATGY 1 cut(s) 2002
XcmI CCANNNNNNNNNTGG 2 cut(s) 364, 2041
XspI CTAG 6 cut(s) 666, 671, 1268, 1592, 1625, 1805
Zsp2I ATGCAT 2 cut(s) 730, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.