RchiOBHm_Chr6g0308661

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000166
Physical Location & Seq
Forward (+)
66727003 .. 66729715
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27755

Sequence Viewer

Length: 2331 bp
ATGGATCCTCTGTTTCTCATTCTACTTCTCTCTCTGGCCATCACTTCTCCAACTTCATCTTCAACCTCCGACTCATTGAGTGAAGGGTCATCTCTCTCTGTAGAGAAACCAGAAGATGTTCTGATTTCACCTGCTGGTGTTTTCACCGCCGGCTTTCATCAAGTTGGCAACAACTCATATTGCATTGCCATTTGGTTCAACGAGCCATCATCATCATCGTCACCAGACTCCTACCAGAACCGAACTGTAGTCTGGATGGCAAATCGTGACCAACCAGTCAACGGGAAGCGCTCAATGCTCTCCCTCCTCAAAACTGGTAATCTCATCTTAACTGATGCTGCTCAATCCCATGTTTGGACTACAACCACCACCTCAATCTCCCCTGCCCGTTTAAGCCTCCACGACTCCGGTAATCTGGTTCTGCTTAATCTGAAGACGAAGGTTGTTCTATGGCAGAGCTTTGACTCCCCAACTGATACCCTTCTTCCACTCCAACCATTCACCAGAACCGCCATACTTGTCTCCTCCAGAAGCCAGAGCAACTTCTCCTCTGGCTTCTATAAGCTCTTCTTCGATAATGATAACCTTCTCCGACTACTCTTTGACGGCCCTGAGATTTCTAGTGTCTACTGGCCCAACCCTGCATATGTGAGCTGGGATAATGAAAGGTCTACTTACAACAGCAGTAGAACTGCCGTGCTTGATTCCTTGGGCAGTTTTGCCTCTTCGGACAATCTGACTTTTCTTGCAGCTGATTATGGTGCAAAGTTGCAGAGAAGATTGACTATTGATTTTGATGGAAATGTTCGATTGTATAGCCGAAAAATGCCAGGAGAGAGTTGGGTGGTTTCATGGCAAGCCTTTTCAGACCCTTGTGTGATTCATGGAATTTGTGGAGCAAACAGCATCTGTAGCTATGATCCCAGTTCTGGTAGGAAATGCTCTTGCCTTTCAGGATATCAGATGGAAAATCATACTGATTGGTCTTCGGGGTGTCAACCGGAATTTCAATTCTCTTATGGAGAAGGTGAGTCTCGTTTTCTCCAACTTGCCCAAGTTGAGTTCTATGGTTACGATTATAATGAGTACCAAAATTATAGCTATGGTGATTGTGAGAAAAGATGCTTGTTGCGAAGTGGGCACAGGTGGAACACTCAAGGTTACTTCTATCTGAGACTGCCCAAGGCTCACCTCTTCTCTAACACAGACATAAAGCCCCTGACGAACCTTGGTTTTAGTTGCACAGAAAAAGTTGTTGATCTCGGGAGGGAGTATGTTAAAGATCGTGTCAGTAAGCCAGAGAAATTCCTGCTTTGGTTTGCCTATGGAGTTGGGGGAGTTGAAATCATTTGTATCATTTTGGTTTGGGGTTTATTGAGCAGTACATTCTCAAAATCAAATGAAGATATTCACGGCTACCTTCTTACTGCAACCGGGTTCAAAAGATTTGGCTACGCTGAGCTCAAGACTGCAACGCGGGGTTTTAGTGAAGAAATTGGGAGAGGAGCAGGAGGAGTTGTTTACAAAGGCATATTGGCTGATCAGCGAGTGGCAGCGATTAAGCTGCTCGGTGAAGCTAACCAAGCAGAAGCCGAATTTCTAGCAGAAGCTAGTACTATTGAGAAGGTGAATCATATGAACTTGATCGAGATGTGGGGATATTGCTCAGAGGGAAAGCACAGGCTTCTTGTTTATGAGTACATGGAGCATGGTTCCTTGGCACAGAAGTTAGCTTCCCATGAGCTTGATTGGGAGAATAGGTTTGAGATTGCTGTCGGCACTGCAAAGGGTCTAGCTTATTTGCACGAAGAGTGCTTGGAGTGGGTTCTACATTGTGATGTAAAGCCTCAAAACATACTGTTGGACTCCAATTTTCAACCAAAGGTTGCAGATTTCGGGCTCTCCAAGATTCTTAACAGAGATGAGCTCAGGAATTCAAGCTTTTCAAGGATACGAGGAACCAGAGGTTACATTGCTCCGGAGTGGGTGTATAATCTTCCCATCACATCCAAAGTGGATGTATACAGCTATGGAGTGGTTGTATTGGAGATGGTGACCGGAAAGAACCCGACAATGGATGTGGAAATCACTGATGGTGAGCAGAGAAGACTAATTATGTGGGTGAGGGAGAAACTAAATGGAACTGAAATTGCGTCCAGAATCGGAGAGATCATAGATCCCTCGTTTGAAGGCAACTATGATGTGGAAAAGATGGAAATTTTGTTAACAGTAGCTTTACATTGTGTGGAAGAAGACAAAGATTCAAGACCAACCATGAGTCAAGTCGTTGAGATGCTTCAACACCACGGCAAAGAATCTCAGTGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

776

Amino Acids

87.25

Weight (kDa)

5.37

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 82 - 167 8.1e-24 D-mannose binding lectin
S_locus_glycop PF00954 254 - 323 5.2e-08 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 495 - 766 4e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 496 - 764 4e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1080
AarI CACCTGC 1 cut(s) 139
AasI GACNNNNNNGTC 1 cut(s) 275
Acc36I ACCTGC 1 cut(s) 139
AccI GTMKAC 3 cut(s) 627, 671, 2022
AccII CGCG 1 cut(s) 1477
AccIII TCCGGA 1 cut(s) 1978
AciI CCGC 3 cut(s) 147, 510, 1477
AclWI GGATC 3 cut(s) 12, 914, 2171
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 6 cut(s) 888, 1004, 1304, 1595, 1933, 2217
AcuI CTGAAG 1 cut(s) 452
AfaI GTAC 4 cut(s) 1088, 1384, 1615, 1700
AfeI AGCGCT 1 cut(s) 290
AfiI CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2074
AjnI CCWGG 1 cut(s) 829
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
Alw21I GWGCWC 2 cut(s) 1464, 1929
Alw26I GTCTC 3 cut(s) 526, 1038, 1168
AlwI GGATC 3 cut(s) 12, 914, 2171
AlwNI CAGNNNCTG 1 cut(s) 909
Ama87I CYCGRG 1 cut(s) 1262
Aor13HI TCCGGA 1 cut(s) 1978
Aor51HI AGCGCT 1 cut(s) 290
AoxI GGCC 3 cut(s) 36, 607, 632
ApeKI GCWGC 4 cut(s) 338, 749, 1553, 1564
ApoI RAATTY 6 cut(s) 888, 1004, 1304, 1595, 1933, 2217
Asp700I GAANNNNTTC 2 cut(s) 117, 1407
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 2 cut(s) 608, 633
AsuC2I CCSGG 1 cut(s) 1435
AvaI CYCGRG 1 cut(s) 1262
BaeGI GKGCMC 1 cut(s) 1143
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 1464, 1902, 1929
BbsI GAAGAC 4 cut(s) 440, 978, 2113, 2259
Bbv12I GWGCWC 2 cut(s) 1464, 1929
BbvI GCAGC 4 cut(s) 325, 761, 1551, 1565
BccI CCATC 9 cut(s) 47, 214, 250, 791, 958, 2009, 2044, 2087, 2206
BceAI ACGGC 4 cut(s) 622, 680, 1429, 2323
BcgI CGANNNNNNTGC 2 cut(s) 1546, 1580
BciT130I CCWGG 1 cut(s) 831
BciVI GTATCC 1 cut(s) 1944
BclI TGATCA 1 cut(s) 1540
BcnI CCSGG 1 cut(s) 1435
BcoDI GTCTC 3 cut(s) 526, 1038, 1168
BfaI CTAG 4 cut(s) 621, 1601, 1611, 1793
BfmI CTRYAG 3 cut(s) 99, 246, 910
BfoI RGCGCY 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 1944
BisI GCNGC 4 cut(s) 339, 750, 1554, 1565
BlpI GCTNAGC 1 cut(s) 1458
BlsI GCNGC 4 cut(s) 340, 751, 1555, 1566
BmcAI AGTACT 1 cut(s) 1615
Bme1390I CCNGG 2 cut(s) 831, 1435
BmeT110I CYCGRG 1 cut(s) 1262
BmgT120I GGNCC 2 cut(s) 608, 633
BmiI GGNNCC 3 cut(s) 6, 1714, 1960
BmrFI CCNGG 2 cut(s) 831, 1435
BmrI ACTGGG 1 cut(s) 918
BmsI GCATC 4 cut(s) 325, 915, 1112, 2283
BmuI ACTGGG 1 cut(s) 918
BpiI GAAGAC 4 cut(s) 440, 978, 2113, 2259
BplI GAGNNNNNCTC 2 cut(s) 1911, 1943
BpmI CTGGAG 1 cut(s) 511
Bpu10I CCTNAGC 1 cut(s) 1928
Bpu1102I GCTNAGC 1 cut(s) 1458
BpuEI CTTGAG 2 cut(s) 1140, 1448
BpuMI CCSGG 1 cut(s) 1435
BsaJI CCNNGG 5 cut(s) 708, 1182, 1228, 1716, 2305
BsaWI WCCGGW 4 cut(s) 407, 1000, 1978, 2057
BsaXI ACNNNNNCTCC 8 cut(s) 1503, 1533, 1745, 1775, 1973, 2003, 2025, 2055
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2074
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 4 cut(s) 275, 319, 635, 924
Bse3DI GCAATG 2 cut(s) 183, 1971
BseAI TCCGGA 1 cut(s) 1978
BseBI CCWGG 1 cut(s) 831
BseDI CCNNGG 5 cut(s) 708, 1182, 1228, 1716, 2305
BseGI GGATG 4 cut(s) 261, 2006, 2023, 2083
BseLI CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2074
BseMI GCAATG 2 cut(s) 183, 1971
BseMII CTCAG 5 cut(s) 603, 1163, 1449, 1680, 1942
BseNI ACTGG 4 cut(s) 275, 319, 635, 924
BseRI GAGGAG 5 cut(s) 296, 514, 538, 1518, 1527
BseSI GKGCMC 1 cut(s) 1143
BseXI GCAGC 4 cut(s) 325, 761, 1551, 1565
BseYI CCCAGC 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 1477
BshFI GGCC 3 cut(s) 38, 609, 634
BsiHKAI GWGCWC 2 cut(s) 1464, 1929
BsiHKCI CYCGRG 1 cut(s) 1262
BsiSI CCGG 6 cut(s) 150, 408, 1001, 1434, 1979, 2058
BslI CCNNNNNNNGG 4 cut(s) 281, 354, 929, 2074
BsmAI GTCTC 3 cut(s) 526, 1038, 1168
BsnI GGCC 3 cut(s) 38, 609, 634
BsoBI CYCGRG 1 cut(s) 1262
Bsp1286I GDGCHC 4 cut(s) 1143, 1464, 1902, 1929
Bsp13I TCCGGA 1 cut(s) 1978
Bsp143I GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
Bsp1720I GCTNAGC 1 cut(s) 1458
BspACI CCGC 3 cut(s) 147, 510, 1477
BspANI GGCC 3 cut(s) 38, 609, 634
BspCNI CTCAG 5 cut(s) 604, 1164, 1450, 1679, 1941
BspEI TCCGGA 1 cut(s) 1978
BspFNI CGCG 1 cut(s) 1477
BspLI GGNNCC 3 cut(s) 6, 1714, 1960
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 3 cut(s) 12, 914, 2171
BspQI GCTCTTC 1 cut(s) 572
BsrDI GCAATG 2 cut(s) 183, 1971
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 4 cut(s) 275, 319, 635, 924
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 5 cut(s) 708, 1182, 1228, 1716, 2305
BssMI GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
BssNAI GTATAC 1 cut(s) 2023
BssT1I CCWWGG 4 cut(s) 708, 1182, 1228, 1716
Bst1107I GTATAC 1 cut(s) 2023
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 3 cut(s) 247, 1860, 2230
Bst6I CTCTTC 4 cut(s) 572, 730, 1199, 1803
BstC8I GCNNGC 2 cut(s) 151, 858
BstDEI CTNAG 6 cut(s) 612, 1172, 1458, 1666, 1928, 2319
BstDSI CCRYGG 1 cut(s) 2305
BstEII GGTNACC 1 cut(s) 2053
BstF5I GGATG 4 cut(s) 261, 2006, 2023, 2083
BstFNI CGCG 1 cut(s) 1477
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 8 cut(s) 7, 922, 1261, 1285, 1543, 1647, 2172, 2179
BstMAI GTCTC 3 cut(s) 526, 1038, 1168
BstMBI GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
BstMWI GCNNNNNNNGC 4 cut(s) 295, 912, 1138, 1583
BstNI CCWGG 1 cut(s) 831
BstPI GGTNACC 1 cut(s) 2053
BstSCI CCNGG 2 cut(s) 829, 1433
BstSFI CTRYAG 3 cut(s) 99, 246, 910
BstSLI GKGCMC 1 cut(s) 1143
BstUI CGCG 1 cut(s) 1477
BstV1I GCAGC 4 cut(s) 325, 761, 1551, 1565
BstV2I GAAGAC 4 cut(s) 440, 978, 2113, 2259
BstX2I RGATCY 2 cut(s) 4, 2176
BstYI RGATCY 2 cut(s) 4, 2176
BstZ17I GTATAC 1 cut(s) 2023
BsuI GTATCC 1 cut(s) 1944
BsuRI GGCC 3 cut(s) 38, 609, 634
BtgI CCRYGG 1 cut(s) 2305
BtsCI GGATG 4 cut(s) 261, 2006, 2023, 2083
BtsI GCAGTG 1 cut(s) 1779
BtsIMutI CAGTG 3 cut(s) 1779, 2088, 2327
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 151, 858
CaiI CAGNNNCTG 1 cut(s) 909
CfoI GCGC 1 cut(s) 291
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 608, 633
CseI GACGC 1 cut(s) 2142
Csp6I GTAC 4 cut(s) 1087, 1383, 1614, 1699
CspCI CAANNNNNGTGG 2 cut(s) 2061, 2096
CviAII CATG 7 cut(s) 350, 852, 884, 1702, 1709, 1739, 2275
CviQI GTAC 4 cut(s) 1087, 1383, 1614, 1699
DdeI CTNAG 6 cut(s) 612, 1172, 1458, 1666, 1928, 2319
DpnI GATC 8 cut(s) 6, 921, 1260, 1284, 1542, 1646, 2171, 2178
DpnII GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 572, 730, 1199, 1803
EarI CTCTTC 4 cut(s) 572, 730, 1199, 1803
Ecl136II GAGCTC 2 cut(s) 1462, 1927
Eco130I CCWWGG 4 cut(s) 708, 1182, 1228, 1716
Eco24I GRGCYC 3 cut(s) 1464, 1902, 1929
Eco32I GATATC 1 cut(s) 959
Eco47III AGCGCT 1 cut(s) 290
Eco53kI GAGCTC 2 cut(s) 1462, 1927
Eco57I CTGAAG 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 1262
Eco91I GGTNACC 1 cut(s) 2053
EcoICRI GAGCTC 2 cut(s) 1462, 1927
EcoO65I GGTNACC 1 cut(s) 2053
EcoRI GAATTC 1 cut(s) 1933
EcoRII CCWGG 1 cut(s) 829
EcoRV GATATC 1 cut(s) 959
EcoT14I CCWWGG 4 cut(s) 708, 1182, 1228, 1716
EcoT38I GRGCYC 3 cut(s) 1464, 1902, 1929
ErhI CCWWGG 4 cut(s) 708, 1182, 1228, 1716
FaeI CATG 7 cut(s) 353, 855, 887, 1705, 1712, 1742, 2278
FalI AAGNNNNNCTT 4 cut(s) 554, 586, 658, 690
FatI CATG 7 cut(s) 349, 851, 883, 1701, 1708, 1738, 2274
FauI CCCGC 1 cut(s) 1470
FauNDI CATATG 2 cut(s) 646, 1635
FbaI TGATCA 1 cut(s) 1540
FblI GTMKAC 3 cut(s) 627, 671, 2022
Fnu4HI GCNGC 4 cut(s) 339, 750, 1554, 1565
FokI GGATG 4 cut(s) 268, 1993, 2030, 2090
FriOI GRGCYC 3 cut(s) 1464, 1902, 1929
Fsp4HI GCNGC 4 cut(s) 339, 750, 1554, 1565
FspBI CTAG 4 cut(s) 621, 1601, 1611, 1793
GlaI GCGC 1 cut(s) 290
GluI GCNGC 4 cut(s) 339, 750, 1554, 1565
GsaI CCCAGC 1 cut(s) 658
GsuI CTGGAG 1 cut(s) 511
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 3 cut(s) 38, 609, 634
HapII CCGG 6 cut(s) 150, 408, 1001, 1434, 1979, 2058
HgaI GACGC 1 cut(s) 2142
HhaI GCGC 1 cut(s) 291
Hin1II CATG 7 cut(s) 353, 855, 887, 1705, 1712, 1742, 2278
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 3 cut(s) 280, 998, 2226
HindII GTYRAC 3 cut(s) 280, 998, 2226
HindIII AAGCTT 1 cut(s) 1939
HpaI GTTAAC 1 cut(s) 2226
HpaII CCGG 6 cut(s) 150, 408, 1001, 1434, 1979, 2058
Hpy166II GTNNAC 7 cut(s) 280, 628, 672, 998, 1522, 2023, 2226
Hpy8I GTNNAC 7 cut(s) 280, 628, 672, 998, 1522, 2023, 2226
HpyAV CCTTC 8 cut(s) 77, 433, 491, 596, 1019, 1430, 1618, 2183
HpyCH4III ACNGT 3 cut(s) 247, 1860, 2230
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 912, 1138, 1583
HpyF3I CTNAG 6 cut(s) 612, 1172, 1458, 1666, 1928, 2319
Hsp92II CATG 7 cut(s) 353, 855, 887, 1705, 1712, 1742, 2278
HspAI GCGC 1 cut(s) 289
Kpn2I TCCGGA 1 cut(s) 1978
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 1540
KspAI GTTAAC 1 cut(s) 2226
Kzo9I GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
LguI GCTCTTC 1 cut(s) 572
LmnI GCTCC 4 cut(s) 896, 1505, 1705, 1981
Lsp1109I GCAGC 4 cut(s) 325, 761, 1551, 1565
LweI GCATC 4 cut(s) 325, 915, 1112, 2283
MaeI CTAG 4 cut(s) 621, 1601, 1611, 1793
MaeIII GTNAC 6 cut(s) 219, 266, 1070, 1160, 1967, 2053
MalI GATC 8 cut(s) 6, 921, 1260, 1284, 1542, 1646, 2171, 2178
MboI GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
MflI RGATCY 2 cut(s) 4, 2176
MhlI GDGCHC 4 cut(s) 1143, 1464, 1902, 1929
MlsI TGGCCA 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 7 cut(s) 65, 221, 398, 458, 1040, 1859, 2287
MmeI TCCRAC 6 cut(s) 74, 93, 517, 616, 1069, 1842
Mox20I TGGCCA 1 cut(s) 38
MroI TCCGGA 1 cut(s) 1978
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 2 cut(s) 117, 1407
MscI TGGCCA 1 cut(s) 38
MseI TTAA 7 cut(s) 329, 392, 426, 1278, 1560, 1914, 2225
MslI CAYNNNNRTG 1 cut(s) 1836
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 6 cut(s) 150, 408, 1001, 1434, 1979, 2058
MspR9I CCNGG 2 cut(s) 831, 1435
MvaI CCWGG 1 cut(s) 831
MvnI CGCG 1 cut(s) 1477
MwoI GCNNNNNNNGC 4 cut(s) 295, 912, 1138, 1583
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 1435
NdeI CATATG 2 cut(s) 646, 1635
NdeII GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 7 cut(s) 353, 855, 887, 1705, 1712, 1742, 2278
NlaIV GGNNCC 3 cut(s) 6, 1714, 1960
NmuCI GTSAC 3 cut(s) 219, 266, 2053
PaqCI CACCTGC 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 572
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 117, 1407
PfeI GAWTC 7 cut(s) 704, 880, 1630, 1909, 2160, 2261, 2315
PkrI GCNGC 4 cut(s) 340, 751, 1555, 1566
PleI GAGTC 7 cut(s) 65, 221, 398, 458, 1039, 1859, 2286
PpsI GAGTC 7 cut(s) 65, 221, 398, 458, 1039, 1859, 2286
PsiI TTATAA 1 cut(s) 1080
Psp124BI GAGCTC 2 cut(s) 1464, 1929
Psp6I CCWGG 1 cut(s) 829
PspEI GGTNACC 1 cut(s) 2053
PspFI CCCAGC 1 cut(s) 654
PspGI CCWGG 1 cut(s) 829
PspN4I GGNNCC 3 cut(s) 6, 1714, 1960
PspPI GGNCC 2 cut(s) 608, 633
PstNI CAGNNNCTG 1 cut(s) 909
PsuI RGATCY 2 cut(s) 4, 2176
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 4 cut(s) 1088, 1384, 1615, 1700
RsaNI GTAC 4 cut(s) 1087, 1383, 1614, 1699
RseI CAYNNNNRTG 1 cut(s) 1836
SacI GAGCTC 2 cut(s) 1464, 1929
SapI GCTCTTC 1 cut(s) 572
SaqAI TTAA 7 cut(s) 329, 392, 426, 1278, 1560, 1914, 2225
SatI GCNGC 4 cut(s) 339, 750, 1554, 1565
Sau3AI GATC 8 cut(s) 4, 919, 1258, 1282, 1540, 1644, 2169, 2176
Sau96I GGNCC 2 cut(s) 608, 633
ScaI AGTACT 1 cut(s) 1615
SchI GAGTC 7 cut(s) 65, 221, 398, 458, 1040, 1859, 2287
ScrFI CCNGG 2 cut(s) 831, 1435
SduI GDGCHC 4 cut(s) 1143, 1464, 1902, 1929
SfaNI GCATC 4 cut(s) 325, 915, 1112, 2283
SfcI CTRYAG 3 cut(s) 99, 246, 910
SmiMI CAYNNNNRTG 1 cut(s) 1836
SmlI CTYRAG 2 cut(s) 1155, 1463
SmoI CTYRAG 2 cut(s) 1155, 1463
SsiI CCGC 3 cut(s) 147, 510, 1477
SspMI CTAG 4 cut(s) 621, 1601, 1611, 1793
SstI GAGCTC 2 cut(s) 1464, 1929
StyD4I CCNGG 2 cut(s) 829, 1433
StyI CCWWGG 4 cut(s) 708, 1182, 1228, 1716
TaaI ACNGT 3 cut(s) 247, 1860, 2230
TaqI TCGA 3 cut(s) 573, 808, 1647
TatI WGTACW 3 cut(s) 1382, 1613, 1698
TfiI GAWTC 7 cut(s) 704, 880, 1630, 1909, 2160, 2261, 2315
Tru1I TTAA 7 cut(s) 329, 392, 426, 1278, 1560, 1914, 2225
Tru9I TTAA 7 cut(s) 329, 392, 426, 1278, 1560, 1914, 2225
TscAI CASTG 3 cut(s) 1786, 2095, 2327
TseFI GTSAC 3 cut(s) 219, 266, 2053
TseI GCWGC 4 cut(s) 338, 749, 1553, 1564
Tsp45I GTSAC 3 cut(s) 219, 266, 2053
TspDTI ATGAA 7 cut(s) 45, 146, 678, 840, 872, 1416, 1652
TspRI CASTG 3 cut(s) 1786, 2095, 2327
XapI RAATTY 6 cut(s) 888, 1004, 1304, 1595, 1933, 2217
XcmI CCANNNNNNNNNTGG 1 cut(s) 837
XmiI GTMKAC 3 cut(s) 627, 671, 2022
XmnI GAANNNNTTC 2 cut(s) 117, 1407
XspI CTAG 4 cut(s) 621, 1601, 1611, 1793
ZrmI AGTACT 1 cut(s) 1615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.