MD04G1137000.v1.1

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000166
Physical Location & Seq
Forward (+)
22451583 .. 22454081
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1137000.v1.1.491

Sequence Viewer

Length: 2499 bp
ATGTATACTCTGATGCCCTTCCCTCTTTCTTATTCACCAAACTTGAAAAAAATGAAGCACCAACATGTTCGCTGTTTTCTTACCTTTGCGTTCATCACTCTGTTCTCCCACTCTTCTTCTGCAATTCCGAAAACCCGAAACCGTCTTTCGAGAGGCTCTTCGATCTCCGTCCAAGACGATTCCGACTTCCTAACATCCCCGGATGAATCCTTCACTTGTGGTTTCTATGGTGTAGGCACAAATGCTTTCTGGTTTTCTATATGGTTTACGAAATCCATAGACAGAGCTGTTGTTTGGACGGCCAACAGAGACAGACCAGTCAACGGCCTAGGCTCCAGACTCTCCCTCCGCCGAGACGGCGCCATGGTCTTGACGGACGTCGATGGCACAGCCGTCTGGGGGACCAACGTGAACTCCAGCAGCTCCAGAGCTGACATGGCGGTGCTTTTGGATTCCGGCAACCTTGTCTTGAAAGACAAGCGAGGTAAAGTTCTGTGGCAGAGTTTTGATATTCCAACTCACACTCTTCTTCCAAACCAACCCTTTACAAAGAGCAAGAAGCTGATATCTACTTTGGGAAAAGGCACTTTTGGGACCGGCTATTTCAGCTTCTCTTTCGACAATGACAATGTTTTGAAGCTCATGTTCGACGGCCCTGACACTTCGAGCTTGTACTGGCCTGATCCGGATTACAACGTTTTCGCCGGCGGTAGAACAAACTACAACAGCAGCAGGGTTGCTGTTTTGGATGAATTGGGGAATTTTTTGTCCAGTGATGAACTGCAATTCACAGCTTCCGACGCGGGTTCAAGTGCGAAAAGGCGGTTGACGATGGATTATGATGGAAACCTCAGGCTTTATAGCCTAGAAAGCACGACAGGGTTGTGGGTGGTCACCTGGGAAGCCATGACGGAGCTGTGTAAGGTGCATGGGATCTGTGGGAGAAATGGGATTTGTATTAACACGCCGCAGCCAAAATGCTCGTGCCCTCCTGGCTACGAAGTAGCTGACACAAGTGACTGGAAAGAGGGTTGCAAGCCTATGTTTAATCGTTCGTATTTGCGGTCCCAGCAAGAGAAATTCGTGCAGATTGAGCAAGTAGATTTCTATGGCTTTGATCTCAATTATAGCGAACCCACCACGTTCGAAAATTGCACGAATCTCTGCTTGGATGATTTCCGGTGCGAAGGATTTAGTTATAGGATAACAGAAGCGAAATGTTTTACAAAGAGTGCTCTTTTCAATGGGTACAAGTCTCCAAATTTTCCGGGGAGCATGTACATAAGACTGCCTCGTAGTGTGAAAACATCGCAGCCCGTCAATCTCAATGCCTCAAATACGTGCAGGAAGAATGTAACAAAGGTTGTTCCTTCGATGTATGGAAGTACCGTGAGAAGGGTAAAATGGGTGTATATGTATTGGTTTGCTTTTGCAATTGGTGCACTTGAAGTTCTTTTCATAGCTTCGGCCTGGTGGCTACTTTTCCGAAGGCACAGTGCAGCAGCTCCAACGGAAGATGGATATCACATAATTTCGAGTCAGTTTAGAAAGTTTCGCTACGCTGAGCTCAAGAAAGCAACCAAAAACTTCAAGGAAGAGCTTGGAAGAGGGGCCTCAGGTGCTGTGTACAAGGGCGTTCTGGCGGACGAAAGAGTGGTGGCCGTGAAGAAACTGGCGGACATATACCATGGTGAAGAAGTGTTTTGGGCAGAAGTCAGCACAATTGGGAAAATTAATCACATGAACCTAGTGAGGATTTGGGGATTCTGTTCCGATAAGAAGCACAAACTCCTCGTCTCTGAGCACGTCGAAAATGGCTCATTGGACAAGCACCTATTCCCCCCAATAAATTTCCTTGGATGGCAAGAAAGGTTTAAAGTTGCAATTGGAATAGCAAAGGGTTTAGCCTATCTTCATCATGAGTGCCTAGAATGGGTTATCCATTGTGATGTGAAGCCCGAGAACATACTTCTAGATGGAAATTTCGAGCCAAAGATTGCAGATTTCGGGCTAGCAAAGTTAACCCAGAGAGGCAACCTGAGCTCAGTTATTTCTCAAATTCGTGGAACGAAAGGGTATATGGCTCCCGAGTGGGCTCAGAACCTTCCGATCACTGCAAAAGTGGATGTTTATAGTTTTGGAGTGGTAATTCTAGAGTTAGTGAAGGGCATTCGCCTTTCAAGTTGGGTAGTTGAGGACACTGATGACCTAGAGACGGAGTTGACAAGGTTCGTGAGGGTAGCGAAGCGGAAAATTCAATGCGGGAAAGATCTGTGGATCGAGGATATGATGGATCCAAGGCTGAATGGGCAGTTCAGCAGGATCCAAGCGGCAAAGATGGTTGAGATTGGGATTTCCTGTGTGGAGGAAGAGAAGAGCAAAAGACCAACCATGGATTCAGTAGTTAAAAGTTTACTAGAATGTGAAGGTGAATCTGCTGTTGCTTCTCCACAAGACACAAGTAACAGCAATTTAAGCTTTAGAGAGAGAAATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

833

Amino Acids

93.91

Weight (kDa)

8.57

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 97 - 187 3.3e-24 D-mannose binding lectin
S_locus_glycop PF00954 257 - 334 7.4e-12 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 530 - 803 4e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 531 - 803 1.8e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 317
AatII GACGTC 1 cut(s) 381
AccB1I GGYRCC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 803
AccIII TCCGGA 1 cut(s) 685
AclI AACGTT 1 cut(s) 696
AclWI GGATC 7 cut(s) 677, 941, 2285, 2288, 2301, 2317, 2330
AcoI YGGCCR 2 cut(s) 300, 1659
AcsI RAATTY 7 cut(s) 760, 1079, 1261, 1849, 1981, 2058, 2253
AcyI GRCGYC 2 cut(s) 360, 378
AfaI GTAC 5 cut(s) 674, 1250, 1280, 1387, 1628
AfiI CCNNNNNNNGG 5 cut(s) 323, 399, 1395, 1933, 2215
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 9 cut(s) 46, 472, 637, 810, 1243, 1448, 1591, 2181, 2258
AjiI CACGTC 1 cut(s) 1807
AjnI CCWGG 3 cut(s) 896, 991, 1469
AjuI GAANNNNNNNTTGG 4 cut(s) 1151, 1183, 2380, 2412
Alw21I GWGCWC 5 cut(s) 1237, 1444, 1569, 1806, 2045
Alw26I GTCTC 5 cut(s) 303, 348, 1260, 1801, 2207
Alw44I GTGCAC 1 cut(s) 1440
AlwI GGATC 7 cut(s) 677, 941, 2285, 2288, 2301, 2317, 2330
AlwNI CAGNNNCTG 1 cut(s) 1622
Ama87I CYCGRG 2 cut(s) 1958, 2087
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 7 cut(s) 300, 325, 652, 677, 1467, 1611, 1659
ApaLI GTGCAC 1 cut(s) 1440
ApeKI GCWGC 6 cut(s) 420, 729, 970, 1312, 1499, 1502
ApoI RAATTY 7 cut(s) 760, 1079, 1261, 1849, 1981, 2058, 2253
ArsI GACNNNNNNTTYG 2 cut(s) 1779, 1811
AseI ATTAAT 1 cut(s) 1734
AspA2I CCTAGG 1 cut(s) 328
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 5 cut(s) 402, 594, 653, 1065, 1611
AsuC2I CCSGG 2 cut(s) 200, 1269
AsuHPI GGTGA 4 cut(s) 27, 886, 1703, 2441
AsuII TTCGAA 1 cut(s) 1146
AsuNHI GCTAGC 1 cut(s) 2011
AvaI CYCGRG 2 cut(s) 1958, 2087
AvaII GGWCC 3 cut(s) 402, 594, 1065
AvrII CCTAGG 1 cut(s) 328
AxyI CCTNAGG 2 cut(s) 851, 1615
BaeGI GKGCMC 2 cut(s) 989, 1444
BamHI GGATCC 2 cut(s) 2293, 2322
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 3 cut(s) 1569, 2045, 2098
BauI CACGAG 1 cut(s) 982
Bbv12I GWGCWC 5 cut(s) 1237, 1444, 1569, 1806, 2045
BbvI GCAGC 6 cut(s) 432, 741, 982, 1324, 1511, 1514
BccI CCATC 8 cut(s) 377, 826, 836, 1511, 1854, 1970, 2284, 2332
BceAI ACGGC 6 cut(s) 315, 340, 373, 377, 667, 1646
BciT130I CCWGG 3 cut(s) 898, 993, 1471
BcnI CCSGG 2 cut(s) 200, 1269
BcoDI GTCTC 5 cut(s) 303, 348, 1260, 1801, 2207
BfaI CTAG 9 cut(s) 329, 866, 1748, 1928, 1973, 2012, 2153, 2210, 2417
BfoI RGCGCY 1 cut(s) 363
BglI GCCNNNNNGGC 2 cut(s) 357, 993
BglII AGATCT 1 cut(s) 2269
BisI GCNGC 8 cut(s) 421, 730, 968, 971, 1313, 1500, 1503, 2331
BlnI CCTAGG 1 cut(s) 328
BlpI GCTNAGC 1 cut(s) 1563
BlsI GCNGC 8 cut(s) 422, 731, 969, 972, 1314, 1501, 1504, 2332
Bme1390I CCNGG 5 cut(s) 200, 898, 993, 1269, 1471
Bme18I GGWCC 3 cut(s) 402, 594, 1065
BmeT110I CYCGRG 2 cut(s) 1958, 2087
BmgBI CACGTC 1 cut(s) 1807
BmgT120I GGNCC 5 cut(s) 402, 594, 653, 1065, 1611
BmiI GGNNCC 9 cut(s) 334, 361, 403, 595, 1067, 1612, 2085, 2295, 2324
BmrFI CCNGG 5 cut(s) 200, 898, 993, 1269, 1471
BmsI GCATC 1 cut(s) 3
BmtI GCTAGC 1 cut(s) 2015
BoxI GACNNNNGTC 1 cut(s) 377
BpmI CTGGAG 3 cut(s) 319, 400, 409
Bpu10I CCTNAGC 1 cut(s) 2039
Bpu1102I GCTNAGC 1 cut(s) 1563
Bpu14I TTCGAA 1 cut(s) 1146
BpuEI CTTGAG 1 cut(s) 1553
BpuMI CCSGG 2 cut(s) 200, 1269
BsaAI YACGTR 1 cut(s) 1341
BsaBI GATNNNNATC 1 cut(s) 1521
BsaHI GRCGYC 2 cut(s) 360, 378
BsaJI CCNNGG 9 cut(s) 198, 328, 363, 897, 1268, 1687, 1855, 2297, 2391
BsaWI WCCGGW 2 cut(s) 685, 1179
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 398, 428
Bsc4I CCNNNNNNNGG 5 cut(s) 323, 399, 1395, 1933, 2215
Bse118I RCCGGY 2 cut(s) 596, 704
Bse1I ACTGG 5 cut(s) 317, 680, 771, 1025, 1677
Bse21I CCTNAGG 2 cut(s) 851, 1615
Bse8I GATNNNNATC 1 cut(s) 1521
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 3 cut(s) 898, 993, 1471
BseDI CCNNGG 9 cut(s) 198, 328, 363, 897, 1268, 1687, 1855, 2297, 2391
BseGI GGATG 6 cut(s) 194, 208, 754, 1177, 1865, 2131
BseJI GATNNNNATC 1 cut(s) 1521
BseLI CCNNNNNNNGG 5 cut(s) 323, 399, 1395, 1933, 2215
BseMII CTCAG 7 cut(s) 865, 1554, 1629, 1791, 2030, 2058, 2111
BseNI ACTGG 5 cut(s) 317, 680, 771, 1025, 1677
BseRI GAGGAG 1 cut(s) 1781
BseSI GKGCMC 2 cut(s) 989, 1444
BseXI GCAGC 6 cut(s) 432, 741, 982, 1324, 1511, 1514
BseYI CCCAGC 1 cut(s) 1068
BsgI GTGCAG 3 cut(s) 1106, 1363, 1518
Bsh1236I CGCG 1 cut(s) 803
BshFI GGCC 7 cut(s) 302, 327, 654, 679, 1469, 1613, 1661
BshNI GGYRCC 1 cut(s) 359
BsiHKAI GWGCWC 5 cut(s) 1237, 1444, 1569, 1806, 2045
BsiHKCI CYCGRG 2 cut(s) 1958, 2087
BsiSI CCGG 7 cut(s) 200, 456, 597, 686, 705, 1180, 1268
BslFI GGGAC 3 cut(s) 415, 607, 1051
BslI CCNNNNNNNGG 5 cut(s) 323, 399, 1395, 1933, 2215
BsmAI GTCTC 5 cut(s) 303, 348, 1260, 1801, 2207
BsmBI CGTCTC 3 cut(s) 348, 1801, 2207
BsmFI GGGAC 3 cut(s) 415, 607, 1051
BsmI GAATGC 1 cut(s) 2169
BsnI GGCC 7 cut(s) 302, 327, 654, 679, 1469, 1613, 1661
BsoBI CYCGRG 2 cut(s) 1958, 2087
Bsp119I TTCGAA 1 cut(s) 1146
Bsp1286I GDGCHC 7 cut(s) 989, 1237, 1444, 1569, 1806, 2045, 2098
Bsp13I TCCGGA 1 cut(s) 685
Bsp1407I TGTACA 2 cut(s) 1278, 1626
Bsp143I GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
Bsp1720I GCTNAGC 1 cut(s) 1563
Bsp19I CCATGG 3 cut(s) 363, 1687, 2391
BspANI GGCC 7 cut(s) 302, 327, 654, 679, 1469, 1613, 1661
BspCNI CTCAG 7 cut(s) 864, 1555, 1628, 1792, 2031, 2057, 2110
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 1 cut(s) 803
BspHI TCATGA 1 cut(s) 1918
BspLI GGNNCC 9 cut(s) 334, 361, 403, 595, 1067, 1612, 2085, 2295, 2324
BspOI GCTAGC 1 cut(s) 2015
BspPI GGATC 7 cut(s) 677, 941, 2285, 2288, 2301, 2317, 2330
BspQI GCTCTTC 3 cut(s) 163, 1590, 2369
BspT104I TTCGAA 1 cut(s) 1146
BspT107I GGYRCC 1 cut(s) 359
BsrFI RCCGGY 2 cut(s) 596, 704
BsrGI TGTACA 2 cut(s) 1278, 1626
BsrI ACTGG 5 cut(s) 317, 680, 771, 1025, 1677
BssAI RCCGGY 2 cut(s) 596, 704
BssECI CCNNGG 9 cut(s) 198, 328, 363, 897, 1268, 1687, 1855, 2297, 2391
BssMI GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
BssNAI GTATAC 1 cut(s) 6
BssNI GRCGYC 2 cut(s) 360, 378
BssSI CACGAG 1 cut(s) 982
BssT1I CCWWGG 6 cut(s) 328, 363, 1687, 1855, 2297, 2391
Bst1107I GTATAC 1 cut(s) 6
Bst2BI CACGAG 1 cut(s) 982
Bst2UI CCWGG 3 cut(s) 898, 993, 1471
Bst4CI ACNGT 3 cut(s) 143, 1390, 1496
Bst6I CTCTTC 7 cut(s) 118, 163, 531, 1590, 1600, 2364, 2369
BstACI GRCGYC 2 cut(s) 360, 378
BstAPI GCANNNNNTGC 1 cut(s) 1439
BstAUI TGTACA 2 cut(s) 1278, 1626
BstBAI YACGTR 1 cut(s) 1341
BstBI TTCGAA 1 cut(s) 1146
BstC8I GCNNGC 3 cut(s) 706, 1037, 2013
BstDEI CTNAG 7 cut(s) 851, 1563, 1615, 1800, 2039, 2044, 2097
BstDSI CCRYGG 3 cut(s) 363, 1687, 2391
BstEII GGTNACC 1 cut(s) 892
BstF5I GGATG 6 cut(s) 194, 208, 754, 1177, 1865, 2131
BstFNI CGCG 1 cut(s) 803
BstH2I RGCGCY 1 cut(s) 363
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 9 cut(s) 165, 685, 936, 1120, 2112, 2272, 2280, 2296, 2325
BstMAI GTCTC 5 cut(s) 303, 348, 1260, 1801, 2207
BstMBI GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
BstNI CCWGG 3 cut(s) 898, 993, 1471
BstNSI RCATGY 2 cut(s) 68, 1279
BstPAI GACNNNNGTC 1 cut(s) 377
BstPI GGTNACC 1 cut(s) 892
BstSCI CCNGG 5 cut(s) 198, 896, 991, 1267, 1469
BstSLI GKGCMC 2 cut(s) 989, 1444
BstUI CGCG 1 cut(s) 803
BstV1I GCAGC 6 cut(s) 432, 741, 982, 1324, 1511, 1514
BstX2I RGATCY 4 cut(s) 933, 2269, 2293, 2322
BstYI RGATCY 4 cut(s) 933, 2269, 2293, 2322
BstZ17I GTATAC 1 cut(s) 6
Bsu36I CCTNAGG 2 cut(s) 851, 1615
BsuRI GGCC 7 cut(s) 302, 327, 654, 679, 1469, 1613, 1661
BtgI CCRYGG 3 cut(s) 363, 1687, 2391
BtgZI GCGATG 1 cut(s) 1293
BtrI CACGTC 1 cut(s) 1807
BtsCI GGATG 6 cut(s) 194, 208, 754, 1177, 1865, 2131
BtsI GCAGTG 1 cut(s) 2112
BtsIMutI CAGTG 4 cut(s) 778, 1501, 2112, 2199
Cac8I GCNNGC 3 cut(s) 706, 1037, 2013
CaiI CAGNNNCTG 1 cut(s) 1622
CciI TCATGA 1 cut(s) 1918
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 2 cut(s) 596, 704
Cfr13I GGNCC 5 cut(s) 402, 594, 653, 1065, 1611
CseI GACGC 1 cut(s) 809
Csp6I GTAC 5 cut(s) 673, 1249, 1279, 1386, 1627
CviQI GTAC 5 cut(s) 673, 1249, 1279, 1386, 1627
DdeI CTNAG 7 cut(s) 851, 1563, 1615, 1800, 2039, 2044, 2097
DinI GGCGCC 1 cut(s) 361
DpnI GATC 9 cut(s) 164, 684, 935, 1119, 2111, 2271, 2279, 2295, 2324
DpnII GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
DraI TTTAAA 1 cut(s) 1876
DrdI GACNNNNNNGTC 1 cut(s) 317
DseDI GACNNNNNNGTC 1 cut(s) 317
EaeI YGGCCR 2 cut(s) 300, 1659
Eam1104I CTCTTC 7 cut(s) 118, 163, 531, 1590, 1600, 2364, 2369
EarI CTCTTC 7 cut(s) 118, 163, 531, 1590, 1600, 2364, 2369
EciI GGCGGA 3 cut(s) 338, 1658, 1691
Ecl136II GAGCTC 2 cut(s) 1567, 2043
Eco130I CCWWGG 6 cut(s) 328, 363, 1687, 1855, 2297, 2391
Eco24I GRGCYC 3 cut(s) 1569, 2045, 2098
Eco32I GATATC 2 cut(s) 567, 1523
Eco47I GGWCC 3 cut(s) 402, 594, 1065
Eco53kI GAGCTC 2 cut(s) 1567, 2043
Eco81I CCTNAGG 2 cut(s) 851, 1615
Eco88I CYCGRG 2 cut(s) 1958, 2087
Eco91I GGTNACC 1 cut(s) 892
EcoICRI GAGCTC 2 cut(s) 1567, 2043
EcoO109I RGGNCCY 1 cut(s) 1611
EcoO65I GGTNACC 1 cut(s) 892
EcoRII CCWGG 3 cut(s) 896, 991, 1469
EcoRV GATATC 2 cut(s) 567, 1523
EcoT14I CCWWGG 6 cut(s) 328, 363, 1687, 1855, 2297, 2391
EcoT38I GRGCYC 3 cut(s) 1569, 2045, 2098
EgeI GGCGCC 1 cut(s) 361
EheI GGCGCC 1 cut(s) 361
ErhI CCWWGG 6 cut(s) 328, 363, 1687, 1855, 2297, 2391
Esp3I CGTCTC 3 cut(s) 348, 1801, 2207
FaqI GGGAC 3 cut(s) 415, 607, 1051
FauI CCCGC 2 cut(s) 796, 2255
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 8 cut(s) 421, 730, 968, 971, 1313, 1500, 1503, 2331
FokI GGATG 6 cut(s) 181, 215, 761, 1184, 1872, 2138
FriOI GRGCYC 3 cut(s) 1569, 2045, 2098
Fsp4HI GCNGC 8 cut(s) 421, 730, 968, 971, 1313, 1500, 1503, 2331
FspBI CTAG 9 cut(s) 329, 866, 1748, 1928, 1973, 2012, 2153, 2210, 2417
GlaI GCGC 1 cut(s) 361
GluI GCNGC 8 cut(s) 421, 730, 968, 971, 1313, 1500, 1503, 2331
GsaI CCCAGC 1 cut(s) 1072
GsuI CTGGAG 3 cut(s) 319, 400, 409
HaeII RGCGCY 1 cut(s) 363
HaeIII GGCC 7 cut(s) 302, 327, 654, 679, 1469, 1613, 1661
HapII CCGG 7 cut(s) 200, 456, 597, 686, 705, 1180, 1268
HgaI GACGC 1 cut(s) 809
HhaI GCGC 1 cut(s) 362
Hin1I GRCGYC 2 cut(s) 360, 378
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 4 cut(s) 322, 828, 2022, 2223
HindII GTYRAC 4 cut(s) 322, 828, 2022, 2223
HindIII AAGCTT 1 cut(s) 2476
HinfI GANTC 9 cut(s) 179, 206, 339, 452, 1159, 1537, 1764, 2396, 2432
HpaI GTTAAC 1 cut(s) 2022
HpaII CCGG 7 cut(s) 200, 456, 597, 686, 705, 1180, 1268
HphI GGTGA 4 cut(s) 27, 886, 1703, 2441
Hpy188I TCNGA 9 cut(s) 12, 129, 184, 799, 1487, 1774, 1801, 2100, 2109
Hpy99I CGWCG 4 cut(s) 383, 653, 803, 1811
HpyAV CCTTC 9 cut(s) 28, 220, 1181, 1380, 1389, 1482, 2114, 2158, 2420
HpyCH4III ACNGT 3 cut(s) 143, 1390, 1496
HpyCH4IV ACGT 6 cut(s) 378, 408, 696, 1142, 1340, 1806
HpyF3I CTNAG 7 cut(s) 851, 1563, 1615, 1800, 2039, 2044, 2097
HpySE526I ACGT 6 cut(s) 378, 408, 696, 1142, 1340, 1806
Hsp92I GRCGYC 2 cut(s) 360, 378
HspAI GCGC 1 cut(s) 360
KasI GGCGCC 1 cut(s) 359
Kpn2I TCCGGA 1 cut(s) 685
KroI GCCGGC 1 cut(s) 704
KroNI GCCGGC 1 cut(s) 706
KspAI GTTAAC 1 cut(s) 2022
Kzo9I GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
LguI GCTCTTC 3 cut(s) 163, 1590, 2369
LmnI GCTCC 6 cut(s) 338, 428, 913, 1272, 1510, 2089
Lsp1109I GCAGC 6 cut(s) 432, 741, 982, 1324, 1511, 1514
LweI GCATC 1 cut(s) 3
MaeI CTAG 9 cut(s) 329, 866, 1748, 1928, 1973, 2012, 2153, 2210, 2417
MaeII ACGT 6 cut(s) 378, 408, 696, 1142, 1340, 1806
MaeIII GTNAC 4 cut(s) 892, 1016, 1354, 2462
MalI GATC 9 cut(s) 164, 684, 935, 1119, 2111, 2271, 2279, 2295, 2324
MboI GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
MfeI CAATTG 3 cut(s) 1434, 1722, 1884
MflI RGATCY 4 cut(s) 933, 2269, 2293, 2322
MhlI GDGCHC 7 cut(s) 989, 1237, 1444, 1569, 1806, 2045, 2098
Mly113I GGCGCC 1 cut(s) 360
MlyI GAGTC 2 cut(s) 333, 1546
MmeI TCCRAC 4 cut(s) 207, 539, 822, 1532
MreI CGCCGGCG 1 cut(s) 704
MroI TCCGGA 1 cut(s) 685
MroNI GCCGGC 1 cut(s) 704
MseI TTAA 7 cut(s) 960, 1047, 1734, 1875, 2021, 2406, 2474
MslI CAYNNNNRTG 3 cut(s) 63, 440, 1947
MspI CCGG 7 cut(s) 200, 456, 597, 686, 705, 1180, 1268
MspR9I CCNGG 5 cut(s) 200, 898, 993, 1269, 1471
MunI CAATTG 3 cut(s) 1434, 1722, 1884
Mva1269I GAATGC 1 cut(s) 2169
MvaI CCWGG 3 cut(s) 898, 993, 1471
MvnI CGCG 1 cut(s) 803
NaeI GCCGGC 1 cut(s) 706
NarI GGCGCC 1 cut(s) 360
NciI CCSGG 2 cut(s) 200, 1269
NcoI CCATGG 3 cut(s) 363, 1687, 2391
NdeII GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
NgoMIV GCCGGC 1 cut(s) 704
NheI GCTAGC 1 cut(s) 2011
NlaIV GGNNCC 9 cut(s) 334, 361, 403, 595, 1067, 1612, 2085, 2295, 2324
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 892, 1016
NspI RCATGY 2 cut(s) 68, 1279
NspV TTCGAA 1 cut(s) 1146
PagI TCATGA 1 cut(s) 1918
PciI ACATGT 1 cut(s) 64
PciSI GCTCTTC 3 cut(s) 163, 1590, 2369
PctI GAATGC 1 cut(s) 2169
PdiI GCCGGC 1 cut(s) 706
PfeI GAWTC 7 cut(s) 179, 206, 452, 1159, 1764, 2396, 2432
PkrI GCNGC 8 cut(s) 422, 731, 969, 972, 1314, 1501, 1504, 2332
PleI GAGTC 2 cut(s) 333, 1545
PluTI GGCGCC 1 cut(s) 363
PpsI GAGTC 2 cut(s) 333, 1545
Ppu21I YACGTR 1 cut(s) 1341
PscI ACATGT 1 cut(s) 64
PshAI GACNNNNGTC 1 cut(s) 377
PshBI ATTAAT 1 cut(s) 1734
Psp124BI GAGCTC 2 cut(s) 1569, 2045
Psp1406I AACGTT 1 cut(s) 696
Psp6I CCWGG 3 cut(s) 896, 991, 1469
PspEI GGTNACC 1 cut(s) 892
PspFI CCCAGC 1 cut(s) 1068
PspGI CCWGG 3 cut(s) 896, 991, 1469
PspN4I GGNNCC 9 cut(s) 334, 361, 403, 595, 1067, 1612, 2085, 2295, 2324
PspPI GGNCC 5 cut(s) 402, 594, 653, 1065, 1611
PstNI CAGNNNCTG 1 cut(s) 1622
PsuI RGATCY 4 cut(s) 933, 2269, 2293, 2322
RsaI GTAC 5 cut(s) 674, 1250, 1280, 1387, 1628
RsaNI GTAC 5 cut(s) 673, 1249, 1279, 1386, 1627
RseI CAYNNNNRTG 3 cut(s) 63, 440, 1947
SacI GAGCTC 2 cut(s) 1569, 2045
SapI GCTCTTC 3 cut(s) 163, 1590, 2369
SaqAI TTAA 7 cut(s) 960, 1047, 1734, 1875, 2021, 2406, 2474
SatI GCNGC 8 cut(s) 421, 730, 968, 971, 1313, 1500, 1503, 2331
Sau3AI GATC 9 cut(s) 162, 682, 933, 1117, 2109, 2269, 2277, 2293, 2322
Sau96I GGNCC 5 cut(s) 402, 594, 653, 1065, 1611
SchI GAGTC 2 cut(s) 333, 1546
ScrFI CCNGG 5 cut(s) 200, 898, 993, 1269, 1471
SduI GDGCHC 7 cut(s) 989, 1237, 1444, 1569, 1806, 2045, 2098
SfaNI GCATC 1 cut(s) 3
SfoI GGCGCC 1 cut(s) 361
SfuI TTCGAA 1 cut(s) 1146
SgrAI CRCCGGYG 1 cut(s) 704
SinI GGWCC 3 cut(s) 402, 594, 1065
SmiMI CAYNNNNRTG 3 cut(s) 63, 440, 1947
SmlI CTYRAG 1 cut(s) 1568
SmoI CTYRAG 1 cut(s) 1568
SspDI GGCGCC 1 cut(s) 359
SspMI CTAG 9 cut(s) 329, 866, 1748, 1928, 1973, 2012, 2153, 2210, 2417
SstI GAGCTC 2 cut(s) 1569, 2045
StyD4I CCNGG 5 cut(s) 198, 896, 991, 1267, 1469
StyI CCWWGG 6 cut(s) 328, 363, 1687, 1855, 2297, 2391
TaaI ACNGT 3 cut(s) 143, 1390, 1496
TaiI ACGT 6 cut(s) 381, 411, 699, 1145, 1343, 1809
TatI WGTACW 3 cut(s) 672, 1278, 1626
TauI GCSGC 2 cut(s) 970, 2333
TfiI GAWTC 7 cut(s) 179, 206, 452, 1159, 1764, 2396, 2432
Tru1I TTAA 7 cut(s) 960, 1047, 1734, 1875, 2021, 2406, 2474
Tru9I TTAA 7 cut(s) 960, 1047, 1734, 1875, 2021, 2406, 2474
TscAI CASTG 4 cut(s) 778, 1501, 2119, 2206
TseFI GTSAC 2 cut(s) 892, 1016
TseI GCWGC 6 cut(s) 420, 729, 970, 1312, 1499, 1502
Tsp45I GTSAC 2 cut(s) 892, 1016
TspDTI ATGAA 8 cut(s) 68, 82, 219, 765, 792, 1447, 1757, 1904
TspGWI ACGGA 5 cut(s) 157, 389, 926, 1526, 2231
TspRI CASTG 4 cut(s) 778, 1501, 2119, 2206
VneI GTGCAC 1 cut(s) 1440
VpaK11BI GGWCC 3 cut(s) 402, 594, 1065
VspI ATTAAT 1 cut(s) 1734
XapI RAATTY 7 cut(s) 760, 1079, 1261, 1849, 1981, 2058, 2253
XbaI TCTAGA 2 cut(s) 1972, 2152
XceI RCATGY 2 cut(s) 68, 1279
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XmaJI CCTAGG 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 9 cut(s) 329, 866, 1748, 1928, 1973, 2012, 2153, 2210, 2417
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.