Rw6G041670

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000166
Physical Location & Seq
Forward (+)
65306827 .. 65309229
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041670.1

Sequence Viewer

Length: 2403 bp
ATGGATCCTCTGTTTCTCATTCTACTTCTCTTTCTGGCCATCACTTCTCCAACTTCATCTTCAACCTCCGACGCATTGAGTGAAGGGTCATCCCTCTCTGTAGAGAAACCAGAAGATGTTCTGATTTCACCTGCTGGTGTTTTCACTGCCGGCTTTCATCAAGTTGGCAACAACTCGTATTGCTTTGCCATTTGGTTCAATGAGCCATCATCATCGTCATCACCAGTCTCCTACCAGAACCGAACTATAGTCTGGATGGCAAATCGTGACCAACCAGTCAACGGGAAGCGCTCAAAGCTCTCCCTCCTCAAAACTGGTAATCTCATCTTAACTGATGCTGCTCAATCCCATGTTTGGACTACAACAACCACCTCAATCTCCCCTGCCCGTTTAAGCCTCCACGACTCCGGTAATCTGGTTCTGCTTAATCTGAAGACGAAGGTTGTTCTATGGCAGAGCTTTGACTCCCCAACTGACACCCTTCTTCCACTCCAACCATTCACCAGAACCGCCATACTTGTCTCCTCCAGAAGCCAGAGCAACTTCTCCTCTGGCTTCTATAAGCTCTTCTTCGACAATGATAACCTTCTCCGGCTACTCTTTGACGGCCCTGAGATTTCTAGTGTCTACTGGCCCAACCCTGCATATGTGAGCTGGGATAATGCAAGGTCTACTTACAACAGCAGTAGAACTGCTGTGCTTGATTCCTTGGGCAGTTTTGCTTCTTCGGACAATCTGACTTTTCTTGCAGTTGATTATGGTGCAAAGTTGCAGAGGAGATTGACTATTGATTTTGATGGAAATGTTCGATTGTATAGCCGAAAAATGCCAGGAGAGAGTTGGGTGGTTTCATGGCAAGCCTTTTCAGACCCTTGTGTGATTCATGGAATTTGTGGAGCTAACAGCCTCTGCAGCTACGATCCCAGTTTTAGGAGGAAATGCTCTTGCCTTCCAGGATATCAGGTGGAAAATCATACTGATTGGTCTTTGGGGTGTCAACCGGAGTTTCAATTCTCTTATGTAGAAGGTGAATCTCGTTTTCTCCAACTTACGCAAGTTGAGTTCTATGGTTACGATTATAATGAGTACCAAAATTATAGCTATGGTGATTGTGAGAAAAGATGCTTGGAACTGGATAACTGCAAGGGGTTTCAGTACACGTTTGATTCTGGTGTTTATAAGTGTAATCCCAAGACGCAGTTGCGAAGTGGGCACAGGTGGAACACTCAAGGTTACTTCTATCTGAGACTGCCCAAGGCTCACCTCTTTTCTAACACAGACATAAAGCCCCTGAAGAACCTTGGTTTTAGTTGCACAGAAAAAGTTGTTGATCTCGAGAGGGAGTATGTTAAAGATCGAGTCAGTAAGCCGGTGAAATTCCTGCTCTGGTTTGCCTATGGAGTTGGGGGAGTTGAAATCATTTGTATCGTTTTGGTTTGGGGTTTGTTGAGCAGTACATTCTCAAAATCAAATGAAGATATTCACGGCTACCTTCTTACTGCAACCGGGTTCAAAAGATTTAGCTACGCTGAGCTCAAGACTGCAACGCGGGGTTTTAGTGAAGAAATTGGGAGAGGAGCAGGAGGAGTTGTTTACAAAGGCATATTGGCTGATCAGCGAGTGGCAGCGATTAAGCTGCTCAGTGAAGCTAACCAAGCAGAAGCAGAATTTCTAGCAGAAGCTAGTACTATTGGGAAGGTGAATCATATGAACTTGATCGAGATGTGGGGATATTGCTCAGAGGGAAGGCACAGGCTTCTTGTTTATGAGTACATGGAGCATGGTTCCTTGGCACAGAAGTTAGCTTCCCATGAGCTTGATTGGGAGATGAGGTTTGAGATTGCTGTCGGCACTGCAAAGGGTCTAGCTTATTTGCACGAAGAGTGCTTGGAGTGGGTTCTACATTGTGATGTAAAGCCTCAAAACATACTGTTGGACTCCAATTTTCAACCAAAGGTTGCAGATTTCGGGCTCTCCAAGATTCTTAACAGAGATGAGCTCAGGAATTCAAGCTTTTCGAGGATACGAGGAACCAGAGGTTACATTGCTCCGGAGTGGGTGTATAATCTTCCCATCACATCCAAAGTGGATGTATACAGCTATGGAGTGGTTGTATTGGAGATGGTGACAGGAAAGAACCCGACAATGGGTGTGGAAATCACTGATGGTGAGCAGAGAAGACTAATTATGTGGGTGAGGGAGAAACTAAATGGAACTGAAATTGCGGCCAGAATCGAAGAGATCATAGATCCCTCGTTTGAAGGCAACTATGATGTGGAAAAGATGGAAATTTTGTTAACAGTAGCTTTACATTGTGTGGAAGAAGACAAAGATTCAAGACCAACCATGAGTCAAGTCGTTGAGATGCTTCAACACCACGGCAAAGAATCTCAGTGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

800

Amino Acids

90.38

Weight (kDa)

5.68

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 82 - 167 1.2e-23 D-mannose binding lectin
S_locus_glycop PF00954 254 - 322 1.1e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 519 - 790 2.4e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 520 - 788 1e-49 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1080, 1179
AarI CACCTGC 1 cut(s) 139
AasI GACNNNNNNGTC 1 cut(s) 275
Acc36I ACCTGC 1 cut(s) 139
AccI GTMKAC 3 cut(s) 627, 671, 2094
AccII CGCG 1 cut(s) 1549
AccIII TCCGGA 1 cut(s) 2050
AciI CCGC 3 cut(s) 510, 1549, 2225
AclWI GGATC 3 cut(s) 12, 914, 2243
AcoI YGGCCR 2 cut(s) 36, 2226
AcsI RAATTY 5 cut(s) 888, 1376, 1667, 2005, 2289
AcuI CTGAAG 2 cut(s) 452, 1313
AfaI GTAC 5 cut(s) 1088, 1157, 1456, 1687, 1772
AfeI AGCGCT 1 cut(s) 290
AfiI CCNNNNNNNGG 5 cut(s) 281, 354, 930, 2146, 2147
AflIII ACRYGT 1 cut(s) 1158
AjnI CCWGG 2 cut(s) 829, 952
AjuI GAANNNNNNNTTGG 4 cut(s) 43, 75, 1109, 1141
Alw21I GWGCWC 2 cut(s) 1536, 2001
Alw26I GTCTC 3 cut(s) 232, 526, 1240
AlwI GGATC 3 cut(s) 12, 914, 2243
AlwNI CAGNNNCTG 1 cut(s) 909
Ama87I CYCGRG 1 cut(s) 1334
Aor13HI TCCGGA 1 cut(s) 2050
Aor51HI AGCGCT 1 cut(s) 290
AoxI GGCC 4 cut(s) 36, 607, 632, 2226
ApeKI GCWGC 4 cut(s) 338, 912, 1625, 1636
ApoI RAATTY 5 cut(s) 888, 1376, 1667, 2005, 2289
Asp700I GAANNNNTTC 2 cut(s) 117, 1479
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 2 cut(s) 608, 633
AsuC2I CCSGG 1 cut(s) 1507
AvaI CYCGRG 1 cut(s) 1334
BaeGI GKGCMC 1 cut(s) 1215
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 1536, 1974, 2001
BbsI GAAGAC 3 cut(s) 440, 2185, 2331
Bbv12I GWGCWC 2 cut(s) 1536, 2001
BbvI GCAGC 4 cut(s) 325, 924, 1623, 1637
BccI CCATC 8 cut(s) 47, 214, 250, 791, 2081, 2116, 2159, 2278
BceAI ACGGC 3 cut(s) 622, 1501, 2395
BcgI CGANNNNNNTGC 2 cut(s) 1618, 1652
BciT130I CCWGG 2 cut(s) 831, 954
BciVI GTATCC 1 cut(s) 2016
BclI TGATCA 1 cut(s) 1612
BcnI CCSGG 1 cut(s) 1507
BcoDI GTCTC 3 cut(s) 232, 526, 1240
BfaI CTAG 4 cut(s) 621, 1673, 1683, 1865
BfmI CTRYAG 3 cut(s) 99, 246, 910
BfoI RGCGCY 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 2016
BisI GCNGC 5 cut(s) 339, 913, 1626, 1637, 2226
BlpI GCTNAGC 1 cut(s) 1530
BlsI GCNGC 5 cut(s) 340, 914, 1627, 1638, 2227
BmcAI AGTACT 1 cut(s) 1687
Bme1390I CCNGG 3 cut(s) 831, 954, 1507
BmeT110I CYCGRG 1 cut(s) 1334
BmgT120I GGNCC 2 cut(s) 608, 633
BmiI GGNNCC 3 cut(s) 6, 1786, 2032
BmrFI CCNGG 3 cut(s) 831, 954, 1507
BmrI ACTGGG 1 cut(s) 918
BmsI GCATC 3 cut(s) 325, 1112, 2355
BmuI ACTGGG 1 cut(s) 918
BpiI GAAGAC 3 cut(s) 440, 2185, 2331
BplI GAGNNNNNCTC 2 cut(s) 1983, 2015
BpmI CTGGAG 1 cut(s) 511
Bpu10I CCTNAGC 1 cut(s) 2000
Bpu1102I GCTNAGC 1 cut(s) 1530
BpuEI CTTGAG 2 cut(s) 1212, 1520
BpuMI CCSGG 1 cut(s) 1507
BsaJI CCNNGG 5 cut(s) 708, 1254, 1300, 1788, 2377
BsaWI WCCGGW 3 cut(s) 407, 1000, 2050
BsaXI ACNNNNNCTCC 8 cut(s) 1575, 1605, 1817, 1847, 2045, 2075, 2097, 2127
Bsc4I CCNNNNNNNGG 5 cut(s) 281, 354, 930, 2146, 2147
Bse118I RCCGGY 2 cut(s) 149, 1369
Bse1I ACTGG 6 cut(s) 224, 275, 319, 635, 924, 1137
Bse3DI GCAATG 1 cut(s) 2043
BseAI TCCGGA 1 cut(s) 2050
BseBI CCWGG 2 cut(s) 831, 954
BseDI CCNNGG 5 cut(s) 708, 1254, 1300, 1788, 2377
BseGI GGATG 4 cut(s) 89, 261, 2078, 2095
BseLI CCNNNNNNNGG 5 cut(s) 281, 354, 930, 2146, 2147
BseMI GCAATG 1 cut(s) 2043
BseMII CTCAG 6 cut(s) 603, 1235, 1521, 1654, 1752, 2014
BseNI ACTGG 6 cut(s) 224, 275, 319, 635, 924, 1137
BseRI GAGGAG 6 cut(s) 296, 514, 538, 790, 1590, 1599
BseSI GKGCMC 1 cut(s) 1215
BseXI GCAGC 4 cut(s) 325, 924, 1623, 1637
BseYI CCCAGC 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 1549
BshFI GGCC 4 cut(s) 38, 609, 634, 2228
BsiHKAI GWGCWC 2 cut(s) 1536, 2001
BsiHKCI CYCGRG 1 cut(s) 1334
BsiSI CCGG 7 cut(s) 150, 408, 592, 1001, 1370, 1506, 2051
BslI CCNNNNNNNGG 5 cut(s) 281, 354, 930, 2146, 2147
BsmAI GTCTC 3 cut(s) 232, 526, 1240
BsnI GGCC 4 cut(s) 38, 609, 634, 2228
BsoBI CYCGRG 1 cut(s) 1334
Bsp1286I GDGCHC 4 cut(s) 1215, 1536, 1974, 2001
Bsp13I TCCGGA 1 cut(s) 2050
Bsp143I GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
Bsp1720I GCTNAGC 1 cut(s) 1530
BspACI CCGC 3 cut(s) 510, 1549, 2225
BspANI GGCC 4 cut(s) 38, 609, 634, 2228
BspCNI CTCAG 6 cut(s) 604, 1236, 1522, 1653, 1751, 2013
BspEI TCCGGA 1 cut(s) 2050
BspFNI CGCG 1 cut(s) 1549
BspLI GGNNCC 3 cut(s) 6, 1786, 2032
BspMAI CTGCAG 1 cut(s) 914
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 3 cut(s) 12, 914, 2243
BspQI GCTCTTC 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 2043
BsrFI RCCGGY 2 cut(s) 149, 1369
BsrI ACTGG 6 cut(s) 224, 275, 319, 635, 924, 1137
BssAI RCCGGY 2 cut(s) 149, 1369
BssECI CCNNGG 5 cut(s) 708, 1254, 1300, 1788, 2377
BssMI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
BssNAI GTATAC 1 cut(s) 2095
BssT1I CCWWGG 4 cut(s) 708, 1254, 1300, 1788
Bst1107I GTATAC 1 cut(s) 2095
Bst2UI CCWGG 2 cut(s) 831, 954
Bst4CI ACNGT 2 cut(s) 1932, 2302
Bst6I CTCTTC 3 cut(s) 572, 1875, 2232
BstC8I GCNNGC 2 cut(s) 151, 858
BstDEI CTNAG 7 cut(s) 612, 1244, 1530, 1640, 1738, 2000, 2391
BstDSI CCRYGG 1 cut(s) 2377
BstF5I GGATG 4 cut(s) 89, 261, 2078, 2095
BstFNI CGCG 1 cut(s) 1549
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 8 cut(s) 7, 922, 1333, 1357, 1615, 1719, 2244, 2251
BstMAI GTCTC 3 cut(s) 232, 526, 1240
BstMBI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
BstMWI GCNNNNNNNGC 4 cut(s) 295, 912, 1210, 1655
BstNI CCWGG 2 cut(s) 831, 954
BstSCI CCNGG 3 cut(s) 829, 952, 1505
BstSFI CTRYAG 3 cut(s) 99, 246, 910
BstSLI GKGCMC 1 cut(s) 1215
BstUI CGCG 1 cut(s) 1549
BstV1I GCAGC 4 cut(s) 325, 924, 1623, 1637
BstV2I GAAGAC 3 cut(s) 440, 2185, 2331
BstX2I RGATCY 2 cut(s) 4, 2248
BstYI RGATCY 2 cut(s) 4, 2248
BstZ17I GTATAC 1 cut(s) 2095
BsuI GTATCC 1 cut(s) 2016
BsuRI GGCC 4 cut(s) 38, 609, 634, 2228
BtgI CCRYGG 1 cut(s) 2377
BtsCI GGATG 4 cut(s) 89, 261, 2078, 2095
BtsI GCAGTG 2 cut(s) 144, 1851
BtsIMutI CAGTG 5 cut(s) 144, 1648, 1851, 2160, 2399
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 151, 858
CaiI CAGNNNCTG 1 cut(s) 909
CfoI GCGC 1 cut(s) 291
Cfr10I RCCGGY 2 cut(s) 149, 1369
Cfr13I GGNCC 2 cut(s) 608, 633
CseI GACGC 2 cut(s) 80, 1204
Csp6I GTAC 5 cut(s) 1087, 1156, 1455, 1686, 1771
CspCI CAANNNNNGTGG 2 cut(s) 2133, 2168
CviAII CATG 7 cut(s) 350, 852, 884, 1774, 1781, 1811, 2347
CviQI GTAC 5 cut(s) 1087, 1156, 1455, 1686, 1771
DdeI CTNAG 7 cut(s) 612, 1244, 1530, 1640, 1738, 2000, 2391
DpnI GATC 8 cut(s) 6, 921, 1332, 1356, 1614, 1718, 2243, 2250
DpnII GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
DrdI GACNNNNNNGTC 1 cut(s) 275
DseDI GACNNNNNNGTC 1 cut(s) 275
EaeI YGGCCR 2 cut(s) 36, 2226
Eam1104I CTCTTC 3 cut(s) 572, 1875, 2232
EarI CTCTTC 3 cut(s) 572, 1875, 2232
Ecl136II GAGCTC 2 cut(s) 1534, 1999
Eco130I CCWWGG 4 cut(s) 708, 1254, 1300, 1788
Eco24I GRGCYC 3 cut(s) 1536, 1974, 2001
Eco32I GATATC 1 cut(s) 959
Eco47III AGCGCT 1 cut(s) 290
Eco53kI GAGCTC 2 cut(s) 1534, 1999
Eco57I CTGAAG 2 cut(s) 452, 1313
Eco88I CYCGRG 1 cut(s) 1334
EcoICRI GAGCTC 2 cut(s) 1534, 1999
EcoRI GAATTC 1 cut(s) 2005
EcoRII CCWGG 2 cut(s) 829, 952
EcoRV GATATC 1 cut(s) 959
EcoT14I CCWWGG 4 cut(s) 708, 1254, 1300, 1788
EcoT38I GRGCYC 3 cut(s) 1536, 1974, 2001
ErhI CCWWGG 4 cut(s) 708, 1254, 1300, 1788
FaeI CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
FalI AAGNNNNNCTT 4 cut(s) 554, 586, 658, 690
FatI CATG 7 cut(s) 349, 851, 883, 1773, 1780, 1810, 2346
FauI CCCGC 1 cut(s) 1542
FauNDI CATATG 2 cut(s) 646, 1707
FbaI TGATCA 1 cut(s) 1612
FblI GTMKAC 3 cut(s) 627, 671, 2094
Fnu4HI GCNGC 5 cut(s) 339, 913, 1626, 1637, 2226
FokI GGATG 4 cut(s) 76, 268, 2065, 2102
FriOI GRGCYC 3 cut(s) 1536, 1974, 2001
Fsp4HI GCNGC 5 cut(s) 339, 913, 1626, 1637, 2226
FspBI CTAG 4 cut(s) 621, 1673, 1683, 1865
GlaI GCGC 1 cut(s) 290
GluI GCNGC 5 cut(s) 339, 913, 1626, 1637, 2226
GsaI CCCAGC 1 cut(s) 658
GsuI CTGGAG 1 cut(s) 511
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 4 cut(s) 38, 609, 634, 2228
HapII CCGG 7 cut(s) 150, 408, 592, 1001, 1370, 1506, 2051
HgaI GACGC 2 cut(s) 80, 1204
HhaI GCGC 1 cut(s) 291
Hin1II CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 3 cut(s) 280, 998, 2298
HindII GTYRAC 3 cut(s) 280, 998, 2298
HindIII AAGCTT 1 cut(s) 2011
HpaI GTTAAC 1 cut(s) 2298
HpaII CCGG 7 cut(s) 150, 408, 592, 1001, 1370, 1506, 2051
Hpy166II GTNNAC 8 cut(s) 280, 628, 672, 998, 1158, 1594, 2095, 2298
Hpy188I TCNGA 8 cut(s) 70, 123, 432, 730, 738, 868, 1245, 1741
Hpy8I GTNNAC 8 cut(s) 280, 628, 672, 998, 1158, 1594, 2095, 2298
Hpy99I CGWCG 1 cut(s) 74
HpyCH4III ACNGT 2 cut(s) 1932, 2302
HpyCH4IV ACGT 1 cut(s) 1160
HpyF10VI GCNNNNNNNGC 4 cut(s) 295, 912, 1210, 1655
HpyF3I CTNAG 7 cut(s) 612, 1244, 1530, 1640, 1738, 2000, 2391
HpySE526I ACGT 1 cut(s) 1160
Hsp92II CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
HspAI GCGC 1 cut(s) 289
Kpn2I TCCGGA 1 cut(s) 2050
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 1612
KspAI GTTAAC 1 cut(s) 2298
Kzo9I GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
LguI GCTCTTC 1 cut(s) 572
LmnI GCTCC 4 cut(s) 896, 1577, 1777, 2053
Lsp1109I GCAGC 4 cut(s) 325, 924, 1623, 1637
LweI GCATC 3 cut(s) 325, 1112, 2355
MaeI CTAG 4 cut(s) 621, 1673, 1683, 1865
MaeII ACGT 1 cut(s) 1160
MaeIII GTNAC 5 cut(s) 266, 1070, 1232, 2039, 2125
MalI GATC 8 cut(s) 6, 921, 1332, 1356, 1614, 1718, 2243, 2250
MboI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
MflI RGATCY 2 cut(s) 4, 2248
MhlI GDGCHC 4 cut(s) 1215, 1536, 1974, 2001
MlsI TGGCCA 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 5 cut(s) 398, 458, 1368, 1931, 2359
MmeI TCCRAC 5 cut(s) 74, 93, 517, 1069, 1914
Mox20I TGGCCA 1 cut(s) 38
MroI TCCGGA 1 cut(s) 2050
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 2 cut(s) 117, 1479
MscI TGGCCA 1 cut(s) 38
MseI TTAA 7 cut(s) 329, 392, 426, 1350, 1632, 1986, 2297
MslI CAYNNNNRTG 1 cut(s) 1908
Msp20I TGGCCA 1 cut(s) 38
MspI CCGG 7 cut(s) 150, 408, 592, 1001, 1370, 1506, 2051
MspR9I CCNGG 3 cut(s) 831, 954, 1507
MvaI CCWGG 2 cut(s) 831, 954
MvnI CGCG 1 cut(s) 1549
MwoI GCNNNNNNNGC 4 cut(s) 295, 912, 1210, 1655
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 1507
NdeI CATATG 2 cut(s) 646, 1707
NdeII GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 7 cut(s) 353, 855, 887, 1777, 1784, 1814, 2350
NlaIV GGNNCC 3 cut(s) 6, 1786, 2032
NmuCI GTSAC 2 cut(s) 266, 2125
PaeR7I CTCGAG 1 cut(s) 1334
PaqCI CACCTGC 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 572
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 117, 1479
PfeI GAWTC 9 cut(s) 704, 880, 1031, 1166, 1702, 1981, 2232, 2333, 2387
PfoI TCCNGGA 1 cut(s) 952
PkrI GCNGC 5 cut(s) 340, 914, 1627, 1638, 2227
PleI GAGTC 5 cut(s) 398, 458, 1367, 1931, 2358
PpsI GAGTC 5 cut(s) 398, 458, 1367, 1931, 2358
PsiI TTATAA 2 cut(s) 1080, 1179
Psp124BI GAGCTC 2 cut(s) 1536, 2001
Psp6I CCWGG 2 cut(s) 829, 952
PspFI CCCAGC 1 cut(s) 654
PspGI CCWGG 2 cut(s) 829, 952
PspN4I GGNNCC 3 cut(s) 6, 1786, 2032
PspPI GGNCC 2 cut(s) 608, 633
PstI CTGCAG 1 cut(s) 914
PstNI CAGNNNCTG 1 cut(s) 909
PsuI RGATCY 2 cut(s) 4, 2248
RsaI GTAC 5 cut(s) 1088, 1157, 1456, 1687, 1772
RsaNI GTAC 5 cut(s) 1087, 1156, 1455, 1686, 1771
RseI CAYNNNNRTG 1 cut(s) 1908
SacI GAGCTC 2 cut(s) 1536, 2001
SapI GCTCTTC 1 cut(s) 572
SaqAI TTAA 7 cut(s) 329, 392, 426, 1350, 1632, 1986, 2297
SatI GCNGC 5 cut(s) 339, 913, 1626, 1637, 2226
Sau3AI GATC 8 cut(s) 4, 919, 1330, 1354, 1612, 1716, 2241, 2248
Sau96I GGNCC 2 cut(s) 608, 633
ScaI AGTACT 1 cut(s) 1687
SchI GAGTC 5 cut(s) 398, 458, 1368, 1931, 2359
ScrFI CCNGG 3 cut(s) 831, 954, 1507
SduI GDGCHC 4 cut(s) 1215, 1536, 1974, 2001
SfaNI GCATC 3 cut(s) 325, 1112, 2355
SfcI CTRYAG 3 cut(s) 99, 246, 910
Sfr274I CTCGAG 1 cut(s) 1334
SlaI CTCGAG 1 cut(s) 1334
SmiMI CAYNNNNRTG 1 cut(s) 1908
SmlI CTYRAG 3 cut(s) 1227, 1334, 1535
SmoI CTYRAG 3 cut(s) 1227, 1334, 1535
SsiI CCGC 3 cut(s) 510, 1549, 2225
SspMI CTAG 4 cut(s) 621, 1673, 1683, 1865
SstI GAGCTC 2 cut(s) 1536, 2001
StyD4I CCNGG 3 cut(s) 829, 952, 1505
StyI CCWWGG 4 cut(s) 708, 1254, 1300, 1788
TaaI ACNGT 2 cut(s) 1932, 2302
TaiI ACGT 1 cut(s) 1163
TaqI TCGA 7 cut(s) 573, 808, 1335, 1357, 1719, 2018, 2235
TatI WGTACW 4 cut(s) 1155, 1454, 1685, 1770
TauI GCSGC 1 cut(s) 2228
TfiI GAWTC 9 cut(s) 704, 880, 1031, 1166, 1702, 1981, 2232, 2333, 2387
Tru1I TTAA 7 cut(s) 329, 392, 426, 1350, 1632, 1986, 2297
Tru9I TTAA 7 cut(s) 329, 392, 426, 1350, 1632, 1986, 2297
TscAI CASTG 5 cut(s) 151, 1648, 1858, 2167, 2399
TseFI GTSAC 2 cut(s) 266, 2125
TseI GCWGC 4 cut(s) 338, 912, 1625, 1636
Tsp45I GTSAC 2 cut(s) 266, 2125
TspDTI ATGAA 6 cut(s) 45, 146, 840, 872, 1488, 1724
TspRI CASTG 5 cut(s) 151, 1648, 1858, 2167, 2399
XapI RAATTY 5 cut(s) 888, 1376, 1667, 2005, 2289
XcmI CCANNNNNNNNNTGG 1 cut(s) 837
XhoI CTCGAG 1 cut(s) 1334
XmiI GTMKAC 3 cut(s) 627, 671, 2094
XmnI GAANNNNTTC 2 cut(s) 117, 1479
XspI CTAG 4 cut(s) 621, 1673, 1683, 1865
ZrmI AGTACT 1 cut(s) 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.