RLG00000024681

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000166
Physical Location & Seq
Reverse (-)
38156720 .. 38158875
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024681

Sequence Viewer

Length: 2064 bp
ATGGCTAACAGAGACAGACCCGTTGGCCTGGAATCCAGAGTTTCGTTGCGTCGAGATGGCTCCATGGTCTTGACTAATCATGTGGATTTCACAAGCATAATCTGGGAGACCAGCACCGGCTCCACTGGTGTTGACAGAGCGGAGCTTCTGAATTCTGGCAACCTTGTTCTGAAAGACCCAAATGGAAGAGGGAATTTTGGTGCTGGATATTTCAATTTCTATTTTGATAATGATAATGTTTTGAAGTTGATGTATGATGGTCCTGACATTTCAAGCTTGTATTGGCCCGACCCTGGTTATAGTGTGTCTGAGAATGGTAGAAATGCAGTCAATAGTAGTAGAAGTGCTGTTTTAGATGAACTTGGTAGTTTTTCATCTAGTGATGAACTGCAGTTCAGTGCTATCGACATGGGTTCGAGAGTGAAAAGGCGGTTGACAATGGATTATGATGGAAATCTAAGACTTTATAGTCTTAATAGCTCAACGGGATTGTGGGTGATCACATGGCAAGCTATGGCGGAGCCTTGTAAGGTTCATGGAATTTGTGGGAGAAATGGGATTTGCATTTATACTCCAGCACCCAAGTGTTCATGTCCTCCTGGCTATGAAGCAGTCGATGCAAGTAACTTGAACTTGGGCTGCAAGCCTAAATTCAAGCGTGCATGCTCGAAGTCCCAAGAGGAGAAATTCGTGCAGATTCCGCAAGTAAATTTCTATGGATTTGATCTCAATTATACTGCACAAATTTCAATTGATGATTCCAGACAGCTCTGCTTAGGGGATTGCCGGTGTGAGGCATTTAGCTATTGGCTAAATGGGGAAGGGAGGTGTTACACCAAAAGTGTTCTTTTCAATGGCTACAAGTCTCCGGATTTTTGGGATACTATATACTTGAGAATGCCTGTGAGTTTTAGTGTGAATACATCAAAACCCCAATATTCCAATGCCTCTATTCCATGCAAGACCAATGGTCCTGCAATGTATGGCACTACCGGTAAAAGGGTGAGATGGGTATATCTGTATAGTTTTGCTTTTGCAGTTGGTGCTGTTGAGTTTCTTTTTATAGTATCAGGTTGGTGGTTACTTTTCAGAAGACATGGTAGTGCTGTAACTCCTGTGGAAGACGGATATCGTGTGATTTCAAGTCAATTTAGAAGGTTTCGCTTCAATGAGCTCAAGAATGCAACCAAAAACTTCCAGGAAGAGCTGGGAAGAGGGGCTTCAGGGTCTGTCTATAAGGGTGTTCTGGAGGATGAAAGAGTAGTGGCTGTCAAGAAATTGGCAGATATTCATCAAGGGGAAGATGTATTTTGGGCAGAAGTAAGCACAATTGGCAAAATCAATCACATGAATCTAGTAAGAATTTGGGGATTTTGCTCAGAAGATAAGCAGAGACTACTGGTTTCTGAGTTTGTTGAAAATGGGTCTTTGGACAAGCACTTGTTCACCTCAAATTTACTTGGATGGAAAGAGAGGTATAAAGTTGCACTGGGGATAGCAAAGGGTTTAGCCTATCTTCACCATGAATGTCTGGAATGGGTTATACATTGTGACGTGAAGCCTGAAAATATACTCCTAGACAGTGATTTTGAGCCAAAGATTGCAGATTTTGGGCTAGCAAAGCTAACTCAGAGAGATAGGCTCAGTTCAATGTTCTCTCAGATTCGTGGAACAAAAGGTTATATGGCCCCGGAGTGGGCTATGAACCTTCGAATTACTGCAAAGGTTGATGTTTACAGTTATGGGGTGGTGATCCTGGAGATAGTGAAGGGCATTCGACTTTCAAGTTGTGTGCTGGAGGATATTGAAGAGGAGCAAGAAGCAGAGCTTACCAAGTTTGTGAGGGAAGCCAAAAGGAAAATTCAACGAGGGGAAGAGTTGTTGATAGAGGATGTTGCTGATCCAAGACTAGAAGGGCAGTTCAGCAGGAACCAAGCAGCAAAGATGGTTAAAATTGGCATTGCCTGTGTGGAGGAAGATAGGAGCAAAAGACCAACAATGGATTCAGTGGTAAAAAGTTTGCTAGAATGTGAAGATGAATCTTACATTCATTCTTCACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

688

Amino Acids

77.6

Weight (kDa)

6.33

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 1 - 65 6.2e-07 D-mannose binding lectin
S_locus_glycop PF00954 126 - 204 4.1e-11 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 399 - 673 3.2e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 400 - 672 1e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 468
AccBSI CCGCTC 1 cut(s) 140
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 4 cut(s) 140, 430, 518, 701
AclWI GGATC 2 cut(s) 1747, 1895
AcuI CTGAAG 1 cut(s) 1206
AfiI CCNNNNNNNGG 5 cut(s) 293, 793, 999, 1695, 1696
AgeI ACCGGT 1 cut(s) 992
AhdI GACNNNNNGTC 1 cut(s) 969
AjiI CACGTC 1 cut(s) 1555
AjnI CCWGG 5 cut(s) 27, 292, 598, 1197, 1755
AjuI GAANNNNNNNTTGG 2 cut(s) 1987, 2019
AleI CACNNNNGTG 1 cut(s) 583
Alw21I GWGCWC 1 cut(s) 1176
Alw26I GTCTC 4 cut(s) 6, 101, 870, 1387
AlwI GGATC 2 cut(s) 1747, 1895
AlwNI CAGNNNCTG 1 cut(s) 1229
Aor13HI TCCGGA 1 cut(s) 868
AoxI GGCC 3 cut(s) 25, 284, 1686
ApeKI GCWGC 2 cut(s) 639, 1937
ArsI GACNNNNNNTTYG 2 cut(s) 1571, 1603
AsiGI ACCGGT 1 cut(s) 992
AspS9I GGNCC 4 cut(s) 260, 285, 971, 1687
AsuC2I CCSGG 1 cut(s) 1691
AsuHPI GGTGA 5 cut(s) 508, 1015, 1438, 1511, 1762
AsuII TTCGAA 1 cut(s) 1711
AsuNHI GCTAGC 1 cut(s) 1615
AvaII GGWCC 2 cut(s) 260, 971
BanII GRGCYC 1 cut(s) 1176
BbsI GAAGAC 2 cut(s) 1099, 1128
Bbv12I GWGCWC 1 cut(s) 1176
BbvI GCAGC 2 cut(s) 626, 1949
BccI CCATC 6 cut(s) 50, 251, 443, 1002, 1458, 1939
BciT130I CCWGG 5 cut(s) 29, 294, 600, 1199, 1757
BciVI GTATCC 1 cut(s) 874
BclI TGATCA 1 cut(s) 498
BcnI CCSGG 1 cut(s) 1691
BcoDI GTCTC 4 cut(s) 6, 101, 870, 1387
BfaI CTAG 6 cut(s) 378, 1355, 1577, 1616, 1910, 2024
BfmI CTRYAG 1 cut(s) 389
BfuI GTATCC 1 cut(s) 874
BisI GCNGC 2 cut(s) 640, 1938
BlsI GCNGC 2 cut(s) 641, 1939
Bme1390I CCNGG 6 cut(s) 29, 294, 600, 1199, 1691, 1757
Bme18I GGWCC 2 cut(s) 260, 971
BmeRI GACNNNNNGTC 1 cut(s) 969
BmgBI CACGTC 1 cut(s) 1555
BmgT120I GGNCC 4 cut(s) 260, 285, 971, 1687
BmiI GGNNCC 5 cut(s) 61, 121, 522, 1689, 1931
BmrFI CCNGG 6 cut(s) 29, 294, 600, 1199, 1691, 1757
BmrI ACTGGG 1 cut(s) 1499
BmsI GCATC 1 cut(s) 607
BmtI GCTAGC 1 cut(s) 1619
BmuI ACTGGG 1 cut(s) 1499
BpiI GAAGAC 2 cut(s) 1099, 1128
BplI GAGNNNNNCTC 2 cut(s) 1626, 1658
BpmI CTGGAG 4 cut(s) 558, 1268, 1778, 1817
Bpu10I CCTNAGC 1 cut(s) 775
Bpu14I TTCGAA 1 cut(s) 1711
BpuEI CTTGAG 2 cut(s) 913, 1160
BpuMI CCSGG 1 cut(s) 1691
BsaBI GATNNNNATC 2 cut(s) 453, 1290
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 3 cut(s) 63, 292, 1689
BsaWI WCCGGW 2 cut(s) 868, 992
Bsc4I CCNNNNNNNGG 5 cut(s) 293, 793, 999, 1695, 1696
Bse118I RCCGGY 3 cut(s) 116, 786, 992
Bse1I ACTGG 3 cut(s) 130, 1404, 1494
Bse3DI GCAATG 2 cut(s) 984, 1959
Bse8I GATNNNNATC 2 cut(s) 453, 1290
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 5 cut(s) 29, 294, 600, 1199, 1757
BseDI CCNNGG 3 cut(s) 63, 292, 1689
BseGI GGATG 3 cut(s) 1258, 1469, 1897
BseJI GATNNNNATC 2 cut(s) 453, 1290
BseLI CCNNNNNNNGG 5 cut(s) 293, 793, 999, 1695, 1696
BseMI GCAATG 2 cut(s) 984, 1959
BseMII CTCAG 6 cut(s) 300, 1392, 1398, 1643, 1657, 1673
BseNI ACTGG 3 cut(s) 130, 1404, 1494
BseRI GAGGAG 2 cut(s) 695, 1826
BseXI GCAGC 2 cut(s) 626, 1949
BseYI CCCAGC 1 cut(s) 1207
BsgI GTGCAG 2 cut(s) 713, 723
BshFI GGCC 3 cut(s) 27, 286, 1688
BshTI ACCGGT 1 cut(s) 992
BsiHKAI GWGCWC 1 cut(s) 1176
BsiSI CCGG 5 cut(s) 117, 787, 869, 993, 1691
BslFI GGGAC 1 cut(s) 658
BslI CCNNNNNNNGG 5 cut(s) 293, 793, 999, 1695, 1696
BsmAI GTCTC 4 cut(s) 6, 101, 870, 1387
BsmFI GGGAC 1 cut(s) 658
BsmI GAATGC 3 cut(s) 903, 1186, 1773
BsnI GGCC 3 cut(s) 27, 286, 1688
Bso31I GGTCTC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 1711
Bsp1286I GDGCHC 1 cut(s) 1176
Bsp13I TCCGGA 1 cut(s) 868
Bsp143I GATC 4 cut(s) 498, 724, 1752, 1900
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 4 cut(s) 140, 430, 518, 701
BspANI GGCC 3 cut(s) 27, 286, 1688
BspCNI CTCAG 6 cut(s) 301, 1391, 1399, 1642, 1656, 1672
BspEI TCCGGA 1 cut(s) 868
BspLI GGNNCC 5 cut(s) 61, 121, 522, 1689, 1931
BspMAI CTGCAG 1 cut(s) 393
BspOI GCTAGC 1 cut(s) 1619
BspPI GGATC 2 cut(s) 1747, 1895
BspQI GCTCTTC 1 cut(s) 1197
BspT104I TTCGAA 1 cut(s) 1711
BspTNI GGTCTC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 140
BsrDI GCAATG 2 cut(s) 984, 1959
BsrFI RCCGGY 3 cut(s) 116, 786, 992
BsrI ACTGG 3 cut(s) 130, 1404, 1494
BssAI RCCGGY 3 cut(s) 116, 786, 992
BssECI CCNNGG 3 cut(s) 63, 292, 1689
BssMI GATC 4 cut(s) 498, 724, 1752, 1900
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 5 cut(s) 29, 294, 600, 1199, 1757
Bst4CI ACNGT 2 cut(s) 1583, 1739
Bst6I CTCTTC 5 cut(s) 181, 1197, 1207, 1803, 1869
BstAPI GCANNNNNTGC 2 cut(s) 617, 1043
BstBI TTCGAA 1 cut(s) 1711
BstC8I GCNNGC 5 cut(s) 510, 644, 660, 664, 1617
BstDEI CTNAG 8 cut(s) 309, 458, 775, 1378, 1407, 1629, 1643, 1659
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 3 cut(s) 1258, 1469, 1897
BstKTI GATC 4 cut(s) 501, 727, 1755, 1903
BstMAI GTCTC 4 cut(s) 6, 101, 870, 1387
BstMBI GATC 4 cut(s) 498, 724, 1752, 1900
BstMWI GCNNNNNNNGC 5 cut(s) 617, 700, 1043, 1332, 1621
BstNI CCWGG 5 cut(s) 29, 294, 600, 1199, 1757
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 6 cut(s) 27, 292, 598, 1197, 1689, 1755
BstSFI CTRYAG 1 cut(s) 389
BstV1I GCAGC 2 cut(s) 626, 1949
BstV2I GAAGAC 2 cut(s) 1099, 1128
BsuI GTATCC 1 cut(s) 874
BsuRI GGCC 3 cut(s) 27, 286, 1688
BtgI CCRYGG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 1555
BtsCI GGATG 3 cut(s) 1258, 1469, 1897
BtsIMutI CAGTG 5 cut(s) 123, 403, 1487, 1588, 2013
Cac8I GCNNGC 5 cut(s) 510, 644, 660, 664, 1617
CaiI CAGNNNCTG 1 cut(s) 1229
Cfr10I RCCGGY 3 cut(s) 116, 786, 992
Cfr13I GGNCC 4 cut(s) 260, 285, 971, 1687
CseI GACGC 1 cut(s) 38
CspAI ACCGGT 1 cut(s) 992
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
DdeI CTNAG 8 cut(s) 309, 458, 775, 1378, 1407, 1629, 1643, 1659
DpnI GATC 4 cut(s) 500, 726, 1754, 1902
DpnII GATC 4 cut(s) 498, 724, 1752, 1900
DrdI GACNNNNNNGTC 1 cut(s) 468
DriI GACNNNNNGTC 1 cut(s) 969
DseDI GACNNNNNNGTC 1 cut(s) 468
Eam1104I CTCTTC 5 cut(s) 181, 1197, 1207, 1803, 1869
Eam1105I GACNNNNNGTC 1 cut(s) 969
EarI CTCTTC 5 cut(s) 181, 1197, 1207, 1803, 1869
EciI GGCGGA 1 cut(s) 533
Ecl136II GAGCTC 1 cut(s) 1174
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 1176
Eco31I GGTCTC 1 cut(s) 101
Eco32I GATATC 1 cut(s) 1130
Eco47I GGWCC 2 cut(s) 260, 971
Eco53kI GAGCTC 1 cut(s) 1174
Eco57I CTGAAG 1 cut(s) 1206
EcoICRI GAGCTC 1 cut(s) 1174
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 5 cut(s) 27, 292, 598, 1197, 1755
EcoRV GATATC 1 cut(s) 1130
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 1176
ErhI CCWWGG 1 cut(s) 63
FalI AAGNNNNNCTT 4 cut(s) 1204, 1236, 1812, 1844
FaqI GGGAC 1 cut(s) 658
FbaI TGATCA 1 cut(s) 498
Fnu4HI GCNGC 2 cut(s) 640, 1938
FokI GGATG 3 cut(s) 1265, 1476, 1904
FriOI GRGCYC 1 cut(s) 1176
Fsp4HI GCNGC 2 cut(s) 640, 1938
FspBI CTAG 6 cut(s) 378, 1355, 1577, 1616, 1910, 2024
GluI GCNGC 2 cut(s) 640, 1938
GsaI CCCAGC 1 cut(s) 1211
GsuI CTGGAG 4 cut(s) 558, 1268, 1778, 1817
HaeIII GGCC 3 cut(s) 27, 286, 1688
HapII CCGG 5 cut(s) 117, 787, 869, 993, 1691
HgaI GACGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 133, 435
HindII GTYRAC 2 cut(s) 133, 435
HindIII AAGCTT 1 cut(s) 274
HinfI GANTC 7 cut(s) 32, 697, 758, 1351, 1663, 2003, 2039
HpaII CCGG 5 cut(s) 117, 787, 869, 993, 1691
HphI GGTGA 5 cut(s) 508, 1015, 1438, 1511, 1762
Hpy166II GTNNAC 4 cut(s) 133, 435, 1446, 1735
Hpy188I TCNGA 8 cut(s) 150, 171, 310, 1091, 1381, 1408, 1632, 1662
Hpy8I GTNNAC 4 cut(s) 133, 435, 1446, 1735
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 5 cut(s) 815, 1149, 1718, 1762, 1907
HpyCH4III ACNGT 2 cut(s) 1583, 1739
HpyCH4IV ACGT 1 cut(s) 1554
HpyF10VI GCNNNNNNNGC 5 cut(s) 617, 700, 1043, 1332, 1621
HpyF3I CTNAG 8 cut(s) 309, 458, 775, 1378, 1407, 1629, 1643, 1659
HpySE526I ACGT 1 cut(s) 1554
Kpn2I TCCGGA 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 498
Kzo9I GATC 4 cut(s) 498, 724, 1752, 1900
LguI GCTCTTC 1 cut(s) 1197
LmnI GCTCC 6 cut(s) 65, 125, 142, 520, 1813, 1983
Lsp1109I GCAGC 2 cut(s) 626, 1949
LweI GCATC 1 cut(s) 607
MaeI CTAG 6 cut(s) 378, 1355, 1577, 1616, 1910, 2024
MaeII ACGT 1 cut(s) 1554
MaeIII GTNAC 5 cut(s) 623, 830, 1080, 1108, 1550
MalI GATC 4 cut(s) 500, 726, 1754, 1902
MbiI CCGCTC 1 cut(s) 140
MboI GATC 4 cut(s) 498, 724, 1752, 1900
MfeI CAATTG 2 cut(s) 750, 1329
MhlI GDGCHC 1 cut(s) 1176
MroI TCCGGA 1 cut(s) 868
MseI TTAA 3 cut(s) 474, 1950, 2062
MslI CAYNNNNRTG 2 cut(s) 583, 1101
MspI CCGG 5 cut(s) 117, 787, 869, 993, 1691
MspR9I CCNGG 6 cut(s) 29, 294, 600, 1199, 1691, 1757
MunI CAATTG 2 cut(s) 750, 1329
Mva1269I GAATGC 3 cut(s) 903, 1186, 1773
MvaI CCWGG 5 cut(s) 29, 294, 600, 1199, 1757
MwoI GCNNNNNNNGC 5 cut(s) 617, 700, 1043, 1332, 1621
NciI CCSGG 1 cut(s) 1691
NcoI CCATGG 1 cut(s) 63
NdeII GATC 4 cut(s) 498, 724, 1752, 1900
NheI GCTAGC 1 cut(s) 1615
NlaIV GGNNCC 5 cut(s) 61, 121, 522, 1689, 1931
NmuCI GTSAC 1 cut(s) 1550
NspI RCATGY 1 cut(s) 666
NspV TTCGAA 1 cut(s) 1711
OliI CACNNNNGTG 1 cut(s) 583
PaeI GCATGC 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 1197
PctI GAATGC 3 cut(s) 903, 1186, 1773
PfeI GAWTC 7 cut(s) 32, 697, 758, 1351, 1663, 2003, 2039
PfoI TCCNGGA 2 cut(s) 1197, 1755
PinAI ACCGGT 1 cut(s) 992
PkrI GCNGC 2 cut(s) 641, 1939
Psp124BI GAGCTC 1 cut(s) 1176
Psp6I CCWGG 5 cut(s) 27, 292, 598, 1197, 1755
PspFI CCCAGC 1 cut(s) 1207
PspGI CCWGG 5 cut(s) 27, 292, 598, 1197, 1755
PspN4I GGNNCC 5 cut(s) 61, 121, 522, 1689, 1931
PspPI GGNCC 4 cut(s) 260, 285, 971, 1687
PstI CTGCAG 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 1229
RseI CAYNNNNRTG 2 cut(s) 583, 1101
SacI GAGCTC 1 cut(s) 1176
SapI GCTCTTC 1 cut(s) 1197
SaqAI TTAA 3 cut(s) 474, 1950, 2062
SatI GCNGC 2 cut(s) 640, 1938
Sau3AI GATC 4 cut(s) 498, 724, 1752, 1900
Sau96I GGNCC 4 cut(s) 260, 285, 971, 1687
ScrFI CCNGG 6 cut(s) 29, 294, 600, 1199, 1691, 1757
SduI GDGCHC 1 cut(s) 1176
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 1 cut(s) 389
SfuI TTCGAA 1 cut(s) 1711
SinI GGWCC 2 cut(s) 260, 971
SmiMI CAYNNNNRTG 2 cut(s) 583, 1101
SmlI CTYRAG 2 cut(s) 892, 1175
SmoI CTYRAG 2 cut(s) 892, 1175
SphI GCATGC 1 cut(s) 666
SsiI CCGC 4 cut(s) 140, 430, 518, 701
SspI AATATT 1 cut(s) 938
SspMI CTAG 6 cut(s) 378, 1355, 1577, 1616, 1910, 2024
SstI GAGCTC 1 cut(s) 1176
StyD4I CCNGG 6 cut(s) 27, 292, 598, 1197, 1689, 1755
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 2 cut(s) 1583, 1739
TaiI ACGT 1 cut(s) 1557
TaqI TCGA 7 cut(s) 52, 405, 416, 615, 668, 1711, 1777
TfiI GAWTC 7 cut(s) 32, 697, 758, 1351, 1663, 2003, 2039
Tru1I TTAA 3 cut(s) 474, 1950, 2062
Tru9I TTAA 3 cut(s) 474, 1950, 2062
TscAI CASTG 5 cut(s) 130, 403, 1494, 1588, 2013
TseFI GTSAC 1 cut(s) 1550
TseI GCWGC 2 cut(s) 639, 1937
Tsp45I GTSAC 1 cut(s) 1550
TspGWI ACGGA 1 cut(s) 1140
TspRI CASTG 5 cut(s) 130, 403, 1494, 1588, 2013
VpaK11BI GGWCC 2 cut(s) 260, 971
XceI RCATGY 1 cut(s) 666
XspI CTAG 6 cut(s) 378, 1355, 1577, 1616, 1910, 2024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.