Rw3G011270

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000166
Physical Location & Seq
Reverse (-)
10911071 .. 10913507
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011270.1

Sequence Viewer

Length: 2391 bp
ATGAAGTACAGAGCTTTTTCAGTCTCCTTTCTATGTCTTCTCTGTTTTGCAACTGCAAAAACCCTAGGTAGTCTAAAGAGAGGATCTTCTCTTTCAGTGGAGGATGATTCAGATTTCCTAACCTCACCAGACAAGTCTTTCACGTGTGGGTTTCATGGGGTTGGCACAAATGCCTACTGGTTCTCAATTTGGTTCACAAGCTCAAAAGACAGGACCATTGTTTGGATGGCCAACAGGGACAAGCCAGTCAATGGCTTGGGGTCTAGAGTCTATCTGAGCCGAGATGGCTCGATGGTCTTGACTGATGTAGATGGAGCCATCATCTGGAAGAGCAGCACCAACTCGAGTACCAATACTGTTGCTGAAAGAGCTGAGCTTTTGGATTCTGGCAACCTTGTTCTGAAGGACCCTAGTGGTAAAATTCTATGGCAAAGCTTTGATTTTCCTACTGGTACTTTGCTCCCAAACCAGCACTTTAGAAAGAGCAATAAGTTGATTTCTAATTTGGGAAGAGGGGATTTTGGTACTGGCTATTTTAGTTTCTATTTTGATAATGATAATGTTTTGAAGTTGATGTATGATGGTCCTGATATTTCTAGCTTGTACTGGCCTGACCTTGATTATAGTGTGTTTCAGAATGGTAGAAATGCTTATAATAGTAGTAGAGTTGCTGTTTTAGATGAACTTGGTAGTTTTTCGTCTAGTGATGAACTGCAATTTAGTGCTTTTGACATGGGTTTGAGAGTGAAAAGGAGGTTGACAATGGATTATGATGGAAATCTAAGACTTTATAGTCTTAATAGCTCAACAGGATTGTGGATAATCACATGGCAAGCTATGGTGGAGCTTTGTAAGGTTCATGGGATATGTGGGCAAAATGGGATTTGTATATATACACCAGGGCCAAAGTGTTCATGTCCTCCTGGCTATGAAGTAGCTGATGCAAGTAACTTGAACTTGGGCTGCAAGCCTAAGTTCAAGCGTACATGCTCGAAGTCCCAAGAAGAGAAATTTGTGCGGATTCCGCAAGTAGATTTCTATGGATTTGATCTCAATTATAGTGTAGCAATTTCAATTGATGATTGCAGACAGCTCTGCTTGGGGGATTGCCGGTGTGAGGCATTTAGCTATAGGCTAAATGGGGAAGGGAGGTGTTACACCAAAAGTGCTCTTTTCAATGGCTACAAGTCTCCAGATTTTGTGGGCAGTATATACTTGAGAATGCCTGTGAGTGTGGAAGCACCATTACTAGTCACCAGGCTCAATGCCCTGGATTCATGTAAGAGTACTGAGGCCCAAAAAATGGGTTCTGCTTCAATGTATGACAATACTAGTAAAAGGGTAAGATGGGCATATCTGTATTGGTTTGCTACTGCAGCTGGTGCAGTTGAAATTCTCTTCATACTTTCAGGTTGGTGGTTACTTTTCAGAAGACATGGCGCTAGAGCTCCTATCGAAGATGGATATCATGTGATTTCGAGTCAATTTAGAATGTTTCGGTACGCTGAGCTCAAGAAGGCGACCAAAACATTCAAGGAAGAGCTGGGAAGAGGGGCTTCTGGGGCTGTGTTTAAGGGTGTTCTGGAAGATGAGAGAGTGGTTGCTGTGAAGAAATTGGCAGACATTTATCAAGGTGAAGATGTGTTTTGGGCGGAAGTAAGCATAATTGGTAAAATCAATCACATGAACCTAGTGAGAATATGGGGATTCTGTTCAGAAGATAAACACAGACTCCTGGTGTCTGAGTTTATTGAAAATGGATCACTAGACAAGCACTTGTTCCCCCCAAATTTTCTTGGATGGCAAGAAAGGTTTAAAGTTGCAATAGGGGTTGCAAAGGGTTTAGCCTATCTTCACCATGAGTGTCTAGAATGGGTTATACATTGTGATGTGAAGCCTGAAAATATACTCTTAGACAGTAACTTTGAGGCAAAGATTGCAGATTTCGGGCTAGCAAAGTTGACTCAGAGAGGCAGCCAGAGCTCTATGTTCTCTAAAGTTCGAGGAACAAAAGGGTATATGGCCCCGGAGTGGGCTCTAAACCTTCCAATCACTGCGAAAGTGGATGTTTACAGTTACGGAGTGGTGATTCTAGAGTTGGTGAAGGGCATTCGACTTTCGAATTGGGCATTGGAGGATAGCGAGGAGCAAGAGGCAGAGCTGACTAGGTGTGTGAGGATAGTGAAAAGGAAGATTCAATGTGGAGAAGATTCATGGATGGAGGATATGTTGGATCCAAGACTAAAAGGGCAGTTCAGCAGGAAACAAGCAGAAAAGATGGTTGAGATTGGTATTTCTTGTGTGGAAGAAGATAGGAGCAGAAGACCGACAATGGATTCAGTATTACAAGATTTGCTAGAATGTGAAGATGAGTCCTATGTGCATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

796

Amino Acids

89.93

Weight (kDa)

6.66

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 163 4e-24 D-mannose binding lectin
S_locus_glycop PF00954 234 - 311 5.7e-10 S-locus glycoprotein domain
PAN_2 PF08276 330 - 388 2.3e-06 PAN-like domain
PAN_1 PF00024 337 - 389 3.7e-06 PAN domain
PK_Tyr_Ser-Thr PF07714 511 - 785 4.3e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 512 - 784 2.9e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
AasI GACNNNNNNGTC 2 cut(s) 245, 792
AccB7I CCANNNNNTGG 4 cut(s) 222, 251, 324, 1303
AciI CCGC 3 cut(s) 1018, 1025, 1652
AclWI GGATC 4 cut(s) 91, 1768, 2228, 2241
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 4 cut(s) 420, 1010, 1392, 1789
AcuI CTGAAG 1 cut(s) 422
AcvI CACGTG 1 cut(s) 144
AfaI GTAC 8 cut(s) 8, 349, 454, 526, 605, 985, 1288, 1502
AfiI CCNNNNNNNGG 7 cut(s) 222, 251, 324, 1117, 1303, 2031, 2032
AflIII ACRYGT 1 cut(s) 143
AhlI ACTAGT 2 cut(s) 1249, 1331
AjnI CCWGG 5 cut(s) 898, 922, 1256, 1269, 1734
AjuI GAANNNNNNNTTGG 2 cut(s) 2114, 2146
Alw21I GWGCWC 4 cut(s) 1171, 1450, 1512, 1985
Alw26I GTCTC 2 cut(s) 28, 1194
AlwI GGATC 4 cut(s) 91, 1768, 2228, 2241
Ama87I CYCGRG 1 cut(s) 343
AoxI GGCC 5 cut(s) 228, 608, 902, 1293, 2022
ApeKI GCWGC 4 cut(s) 333, 963, 1376, 1974
ApoI RAATTY 4 cut(s) 420, 1010, 1392, 1789
ArsI GACNNNNNNTTYG 2 cut(s) 1907, 1939
AspA2I CCTAGG 1 cut(s) 64
AspLEI GCGC 1 cut(s) 1442
AspS9I GGNCC 6 cut(s) 213, 406, 584, 902, 1294, 2023
AsuC2I CCSGG 1 cut(s) 2027
AsuHPI GGTGA 6 cut(s) 117, 1246, 1646, 1847, 2098, 2113
AsuII TTCGAA 1 cut(s) 2120
AsuNHI GCTAGC 1 cut(s) 1951
AvaI CYCGRG 1 cut(s) 343
AvaII GGWCC 3 cut(s) 213, 406, 584
AvrII CCTAGG 1 cut(s) 64
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BalI TGGCCA 1 cut(s) 230
BamHI GGATCC 1 cut(s) 2233
BanII GRGCYC 4 cut(s) 1450, 1512, 1985, 2038
BarI GAAGNNNNNNTAC 2 cut(s) 1230, 1262
BbrPI CACGTG 1 cut(s) 144
BbsI GAAGAC 3 cut(s) 29, 1438, 2329
Bbv12I GWGCWC 4 cut(s) 1171, 1450, 1512, 1985
BbvI GCAGC 4 cut(s) 345, 950, 1388, 1986
BciT130I CCWGG 5 cut(s) 900, 924, 1258, 1271, 1736
BcnI CCSGG 1 cut(s) 2027
BcoDI GTCTC 2 cut(s) 28, 1194
BcuI ACTAGT 2 cut(s) 1249, 1331
BfmI CTRYAG 2 cut(s) 1129, 1374
BfoI RGCGCY 1 cut(s) 1443
BglI GCCNNNNNGGC 1 cut(s) 285
BisI GCNGC 4 cut(s) 334, 964, 1377, 1975
BlnI CCTAGG 1 cut(s) 64
BlpI GCTNAGC 2 cut(s) 372, 1506
BlsI GCNGC 4 cut(s) 335, 965, 1378, 1976
BmcAI AGTACT 1 cut(s) 1288
Bme1390I CCNGG 6 cut(s) 900, 924, 1258, 1271, 1736, 2027
Bme18I GGWCC 3 cut(s) 213, 406, 584
BmeT110I CYCGRG 1 cut(s) 343
BmgT120I GGNCC 6 cut(s) 213, 406, 584, 902, 1294, 2023
BmiI GGNNCC 4 cut(s) 316, 408, 2025, 2235
BmrFI CCNGG 6 cut(s) 900, 924, 1258, 1271, 1736, 2027
BmsI GCATC 1 cut(s) 931
BmtI GCTAGC 1 cut(s) 1955
BpiI GAAGAC 3 cut(s) 29, 1438, 2329
BpmI CTGGAG 1 cut(s) 1176
Bpu1102I GCTNAGC 2 cut(s) 372, 1506
Bpu14I TTCGAA 1 cut(s) 2120
BpuEI CTTGAG 2 cut(s) 1237, 1496
BpuMI CCSGG 1 cut(s) 2027
BsaAI YACGTR 1 cut(s) 144
BsaBI GATNNNNATC 2 cut(s) 777, 1464
BsaJI CCNNGG 4 cut(s) 64, 899, 1269, 2025
BsaXI ACNNNNNCTCC 4 cut(s) 1716, 1746, 2213, 2243
Bsc4I CCNNNNNNNGG 7 cut(s) 222, 251, 324, 1117, 1303, 2031, 2032
Bse118I RCCGGY 1 cut(s) 1110
Bse1I ACTGG 5 cut(s) 182, 245, 454, 532, 611
Bse8I GATNNNNATC 2 cut(s) 777, 1464
BseBI CCWGG 5 cut(s) 900, 924, 1258, 1271, 1736
BseDI CCNNGG 4 cut(s) 64, 899, 1269, 2025
BseGI GGATG 5 cut(s) 109, 231, 1805, 2071, 2223
BseJI GATNNNNATC 2 cut(s) 777, 1464
BseLI CCNNNNNNNGG 7 cut(s) 222, 251, 324, 1117, 1303, 2031, 2032
BseMII CTCAG 6 cut(s) 266, 363, 1281, 1497, 1734, 1979
BseNI ACTGG 5 cut(s) 182, 245, 454, 532, 611
BseRI GAGGAG 1 cut(s) 2159
BseXI GCAGC 4 cut(s) 345, 950, 1388, 1986
BseYI CCCAGC 1 cut(s) 1543
BsgI GTGCAG 1 cut(s) 1404
BshFI GGCC 5 cut(s) 230, 610, 904, 1295, 2024
BsiHKAI GWGCWC 4 cut(s) 1171, 1450, 1512, 1985
BsiHKCI CYCGRG 1 cut(s) 343
BsiSI CCGG 2 cut(s) 1111, 2027
BslFI GGGAC 2 cut(s) 251, 982
BslI CCNNNNNNNGG 7 cut(s) 222, 251, 324, 1117, 1303, 2031, 2032
BsmAI GTCTC 2 cut(s) 28, 1194
BsmFI GGGAC 2 cut(s) 251, 982
BsmI GAATGC 2 cut(s) 1227, 2109
BsnI GGCC 5 cut(s) 230, 610, 904, 1295, 2024
BsoBI CYCGRG 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 2120
Bsp1286I GDGCHC 5 cut(s) 1171, 1450, 1512, 1985, 2038
Bsp143I GATC 4 cut(s) 83, 1048, 1760, 2233
Bsp1720I GCTNAGC 2 cut(s) 372, 1506
BspACI CCGC 3 cut(s) 1018, 1025, 1652
BspANI GGCC 5 cut(s) 230, 610, 904, 1295, 2024
BspCNI CTCAG 6 cut(s) 267, 364, 1282, 1498, 1735, 1978
BspLI GGNNCC 4 cut(s) 316, 408, 2025, 2235
BspMAI CTGCAG 1 cut(s) 1378
BspOI GCTAGC 1 cut(s) 1955
BspPI GGATC 4 cut(s) 91, 1768, 2228, 2241
BspQI GCTCTTC 2 cut(s) 323, 1533
BspT104I TTCGAA 1 cut(s) 2120
BsrFI RCCGGY 1 cut(s) 1110
BsrI ACTGG 5 cut(s) 182, 245, 454, 532, 611
BssAI RCCGGY 1 cut(s) 1110
BssECI CCNNGG 4 cut(s) 64, 899, 1269, 2025
BssMI GATC 4 cut(s) 83, 1048, 1760, 2233
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 5 cut(s) 900, 924, 1258, 1271, 1736
Bst4CI ACNGT 3 cut(s) 358, 1919, 2075
Bst6I CTCTTC 6 cut(s) 323, 505, 999, 1403, 1533, 1543
BstAPI GCANNNNNTGC 2 cut(s) 1382, 1937
BstBAI YACGTR 1 cut(s) 144
BstBI TTCGAA 1 cut(s) 2120
BstC8I GCNNGC 3 cut(s) 834, 968, 1953
BstDEI CTNAG 9 cut(s) 275, 372, 782, 972, 1290, 1506, 1743, 1912, 1965
BstF5I GGATG 5 cut(s) 109, 231, 1805, 2071, 2223
BstH2I RGCGCY 1 cut(s) 1443
BstHHI GCGC 1 cut(s) 1442
BstKTI GATC 4 cut(s) 86, 1051, 1763, 2236
BstMAI GTCTC 2 cut(s) 28, 1194
BstMBI GATC 4 cut(s) 83, 1048, 1760, 2233
BstMWI GCNNNNNNNGC 8 cut(s) 285, 368, 1024, 1376, 1382, 1562, 1937, 1980
BstNI CCWGG 5 cut(s) 900, 924, 1258, 1271, 1736
BstNSI RCATGY 1 cut(s) 990
BstSCI CCNGG 6 cut(s) 898, 922, 1256, 1269, 1734, 2025
BstSFI CTRYAG 2 cut(s) 1129, 1374
BstV1I GCAGC 4 cut(s) 345, 950, 1388, 1986
BstV2I GAAGAC 3 cut(s) 29, 1438, 2329
BstX2I RGATCY 2 cut(s) 83, 2233
BstYI RGATCY 2 cut(s) 83, 2233
BsuRI GGCC 5 cut(s) 230, 610, 904, 1295, 2024
BtsCI GGATG 5 cut(s) 109, 231, 1805, 2071, 2223
BtsI GCAGTG 1 cut(s) 2052
BtsIMutI CAGTG 2 cut(s) 102, 2052
Cac8I GCNNGC 3 cut(s) 834, 968, 1953
CfoI GCGC 1 cut(s) 1442
Cfr10I RCCGGY 1 cut(s) 1110
Cfr13I GGNCC 6 cut(s) 213, 406, 584, 902, 1294, 2023
Csp6I GTAC 8 cut(s) 7, 348, 453, 525, 604, 984, 1287, 1501
CviQI GTAC 8 cut(s) 7, 348, 453, 525, 604, 984, 1287, 1501
DdeI CTNAG 9 cut(s) 275, 372, 782, 972, 1290, 1506, 1743, 1912, 1965
DpnI GATC 4 cut(s) 85, 1050, 1762, 2235
DpnII GATC 4 cut(s) 83, 1048, 1760, 2233
DraI TTTAAA 1 cut(s) 1816
DrdI GACNNNNNNGTC 2 cut(s) 245, 792
DseDI GACNNNNNNGTC 2 cut(s) 245, 792
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 6 cut(s) 323, 505, 999, 1403, 1533, 1543
EarI CTCTTC 6 cut(s) 323, 505, 999, 1403, 1533, 1543
EciI GGCGGA 1 cut(s) 1667
Ecl136II GAGCTC 3 cut(s) 1448, 1510, 1983
Eco130I CCWWGG 1 cut(s) 64
Eco24I GRGCYC 4 cut(s) 1450, 1512, 1985, 2038
Eco32I GATATC 1 cut(s) 1466
Eco47I GGWCC 3 cut(s) 213, 406, 584
Eco53kI GAGCTC 3 cut(s) 1448, 1510, 1983
Eco57I CTGAAG 1 cut(s) 422
Eco72I CACGTG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 343
EcoICRI GAGCTC 3 cut(s) 1448, 1510, 1983
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 5 cut(s) 898, 922, 1256, 1269, 1734
EcoRV GATATC 1 cut(s) 1466
EcoT14I CCWWGG 1 cut(s) 64
EcoT38I GRGCYC 4 cut(s) 1450, 1512, 1985, 2038
ErhI CCWWGG 1 cut(s) 64
FalI AAGNNNNNCTT 2 cut(s) 1540, 1572
FaqI GGGAC 2 cut(s) 251, 982
Fnu4HI GCNGC 4 cut(s) 334, 964, 1377, 1975
FokI GGATG 5 cut(s) 116, 238, 1812, 2078, 2230
FriOI GRGCYC 4 cut(s) 1450, 1512, 1985, 2038
Fsp4HI GCNGC 4 cut(s) 334, 964, 1377, 1975
GlaI GCGC 1 cut(s) 1441
GluI GCNGC 4 cut(s) 334, 964, 1377, 1975
GsaI CCCAGC 1 cut(s) 1547
GsuI CTGGAG 1 cut(s) 1176
HaeII RGCGCY 1 cut(s) 1443
HaeIII GGCC 5 cut(s) 230, 610, 904, 1295, 2024
HapII CCGG 2 cut(s) 1111, 2027
HhaI GCGC 1 cut(s) 1442
Hin6I GCGC 1 cut(s) 1440
HinP1I GCGC 1 cut(s) 1440
HincII GTYRAC 2 cut(s) 759, 1962
HindII GTYRAC 2 cut(s) 759, 1962
HindIII AAGCTT 1 cut(s) 433
HpaII CCGG 2 cut(s) 1111, 2027
HphI GGTGA 6 cut(s) 117, 1246, 1646, 1847, 2098, 2113
Hpy166II GTNNAC 4 cut(s) 195, 759, 1962, 2071
Hpy188I TCNGA 8 cut(s) 112, 276, 402, 636, 1430, 1717, 1744, 1968
Hpy188III TCNNGA 9 cut(s) 264, 298, 325, 587, 1193, 1513, 1583, 1868, 2093
Hpy8I GTNNAC 4 cut(s) 195, 759, 1962, 2071
HpyAV CCTTC 5 cut(s) 397, 1139, 1510, 2054, 2098
HpyCH4III ACNGT 3 cut(s) 358, 1919, 2075
HpyCH4IV ACGT 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 8 cut(s) 285, 368, 1024, 1376, 1382, 1562, 1937, 1980
HpyF3I CTNAG 9 cut(s) 275, 372, 782, 972, 1290, 1506, 1743, 1912, 1965
HpySE526I ACGT 1 cut(s) 143
HspAI GCGC 1 cut(s) 1440
Kzo9I GATC 4 cut(s) 83, 1048, 1760, 2233
LguI GCTCTTC 2 cut(s) 323, 1533
LmnI GCTCC 6 cut(s) 314, 465, 844, 1453, 2146, 2316
Lsp1109I GCAGC 4 cut(s) 345, 950, 1388, 1986
LweI GCATC 1 cut(s) 931
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 6 cut(s) 947, 1154, 1252, 1419, 1919, 2075
MalI GATC 4 cut(s) 85, 1050, 1762, 2235
MboI GATC 4 cut(s) 83, 1048, 1760, 2233
MfeI CAATTG 1 cut(s) 1074
MflI RGATCY 2 cut(s) 83, 2233
MhlI GDGCHC 5 cut(s) 1171, 1450, 1512, 1985, 2038
MlsI TGGCCA 1 cut(s) 230
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 5 cut(s) 276, 1489, 1725, 1957, 2381
MmeI TCCRAC 1 cut(s) 2211
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 3 cut(s) 798, 1572, 1815
MslI CAYNNNNRTG 1 cut(s) 1887
Msp20I TGGCCA 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 1379
MspI CCGG 2 cut(s) 1111, 2027
MspR9I CCNGG 6 cut(s) 900, 924, 1258, 1271, 1736, 2027
MunI CAATTG 1 cut(s) 1074
Mva1269I GAATGC 2 cut(s) 1227, 2109
MvaI CCWGG 5 cut(s) 900, 924, 1258, 1271, 1736
MwoI GCNNNNNNNGC 8 cut(s) 285, 368, 1024, 1376, 1382, 1562, 1937, 1980
NciI CCSGG 1 cut(s) 2027
NdeII GATC 4 cut(s) 83, 1048, 1760, 2233
NheI GCTAGC 1 cut(s) 1951
NlaIV GGNNCC 4 cut(s) 316, 408, 2025, 2235
NmeAIII GCCGAG 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 1252
NspI RCATGY 1 cut(s) 990
NspV TTCGAA 1 cut(s) 2120
PaeR7I CTCGAG 1 cut(s) 343
PciSI GCTCTTC 2 cut(s) 323, 1533
PctI GAATGC 2 cut(s) 1227, 2109
PfeI GAWTC 9 cut(s) 107, 383, 1021, 1274, 1707, 2089, 2194, 2210, 2336
PflMI CCANNNNNTGG 4 cut(s) 222, 251, 324, 1303
PkrI GCNGC 4 cut(s) 335, 965, 1378, 1976
PleI GAGTC 5 cut(s) 275, 1488, 1725, 1957, 2380
PmaCI CACGTG 1 cut(s) 144
PmlI CACGTG 1 cut(s) 144
PpsI GAGTC 5 cut(s) 275, 1488, 1725, 1957, 2380
Ppu21I YACGTR 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 406
PsiI TTATAA 1 cut(s) 654
Psp124BI GAGCTC 3 cut(s) 1450, 1512, 1985
Psp5II RGGWCCY 1 cut(s) 406
Psp6I CCWGG 5 cut(s) 898, 922, 1256, 1269, 1734
PspCI CACGTG 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 1543
PspGI CCWGG 5 cut(s) 898, 922, 1256, 1269, 1734
PspN4I GGNNCC 4 cut(s) 316, 408, 2025, 2235
PspPI GGNCC 6 cut(s) 213, 406, 584, 902, 1294, 2023
PspPPI RGGWCCY 1 cut(s) 406
PspXI VCTCGAGB 1 cut(s) 343
PstI CTGCAG 1 cut(s) 1378
PsuI RGATCY 2 cut(s) 83, 2233
PvuII CAGCTG 1 cut(s) 1379
RsaI GTAC 8 cut(s) 8, 349, 454, 526, 605, 985, 1288, 1502
RsaNI GTAC 8 cut(s) 7, 348, 453, 525, 604, 984, 1287, 1501
RseI CAYNNNNRTG 1 cut(s) 1887
SacI GAGCTC 3 cut(s) 1450, 1512, 1985
SapI GCTCTTC 2 cut(s) 323, 1533
SaqAI TTAA 3 cut(s) 798, 1572, 1815
SatI GCNGC 4 cut(s) 334, 964, 1377, 1975
Sau3AI GATC 4 cut(s) 83, 1048, 1760, 2233
Sau96I GGNCC 6 cut(s) 213, 406, 584, 902, 1294, 2023
ScaI AGTACT 1 cut(s) 1288
SchI GAGTC 5 cut(s) 276, 1489, 1725, 1957, 2381
ScrFI CCNGG 6 cut(s) 900, 924, 1258, 1271, 1736, 2027
SduI GDGCHC 5 cut(s) 1171, 1450, 1512, 1985, 2038
SfaNI GCATC 1 cut(s) 931
SfcI CTRYAG 2 cut(s) 1129, 1374
Sfr274I CTCGAG 1 cut(s) 343
SfuI TTCGAA 1 cut(s) 2120
SinI GGWCC 3 cut(s) 213, 406, 584
SlaI CTCGAG 1 cut(s) 343
SmiMI CAYNNNNRTG 1 cut(s) 1887
SmlI CTYRAG 3 cut(s) 343, 1216, 1511
SmoI CTYRAG 3 cut(s) 343, 1216, 1511
SpeI ACTAGT 2 cut(s) 1249, 1331
SsiI CCGC 3 cut(s) 1018, 1025, 1652
SstI GAGCTC 3 cut(s) 1450, 1512, 1985
StyD4I CCNGG 6 cut(s) 898, 922, 1256, 1269, 1734, 2025
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 358, 1919, 2075
TaiI ACGT 1 cut(s) 146
TaqI TCGA 8 cut(s) 290, 344, 992, 1455, 1478, 2002, 2113, 2120
TaqII GACCGA 1 cut(s) 2341
TatI WGTACW 3 cut(s) 6, 603, 1286
TfiI GAWTC 9 cut(s) 107, 383, 1021, 1274, 1707, 2089, 2194, 2210, 2336
Tru1I TTAA 3 cut(s) 798, 1572, 1815
Tru9I TTAA 3 cut(s) 798, 1572, 1815
TscAI CASTG 2 cut(s) 102, 2059
TseFI GTSAC 1 cut(s) 1252
TseI GCWGC 4 cut(s) 333, 963, 1376, 1974
Tsp45I GTSAC 1 cut(s) 1252
TspGWI ACGGA 1 cut(s) 2094
TspRI CASTG 2 cut(s) 102, 2059
Van91I CCANNNNNTGG 4 cut(s) 222, 251, 324, 1303
VpaK11BI GGWCC 3 cut(s) 213, 406, 584
XapI RAATTY 4 cut(s) 420, 1010, 1392, 1789
XbaI TCTAGA 3 cut(s) 263, 1867, 2092
XceI RCATGY 1 cut(s) 990
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 343
XmaJI CCTAGG 1 cut(s) 64
ZrmI AGTACT 1 cut(s) 1288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.