MD15G1076400.v1.1

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000166
Physical Location & Seq
Forward (+)
5186601 .. 5189021
2421 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1076400.v1.1.491

Sequence Viewer

Length: 2421 bp
ATGTTGTATTCATTCATTGGTCACATGGGCACCTTGTTTCTCATTTTTCTTCTTTCTCTAGCCATTAGTCCCCTTCCTTCGTCTTCAGCATCGGACACTTTGCGTAGAGGCTCATCCCTCTCTGTGGAGAAACCCAATGATGTCTTAATCTCACCGGATGGGGTTTTCACTGCCGGCTTTCACCAAGTTGGCGACAACTCATTTTGCTTCGCCATTTGGTTCACCGAGCCTTCTTCCAATCACTACCAAAACCGCGCGGTTGTTTGGATGGCGAATCGCGACCAACCGGTGAACGGGAAATACTCAAAGCTCTCACTCCAAAGTTCTGGCAATCTCATCTTAACTGATGCCGGCCAGTCTATTGTTTGGGCAACAACCACTACGTCACTCTCGGCAGCTCACTTGTCCCTCCAGAACTCAGGCAATCTGGTTTTACTAAACCTTGATCTTGTCGTTTTATGGCAGAGCTTTGATTTTCCAACCGACACCCTTCTTCCACATCAACCGCTCACCCGAAACACAATGATTGTCTCTATGAGAAGCCAGAGCAACTTCTCCTCTGGCTTCTACAAGCTTTTCTTTGACAATGACAATCTTCTGCGTCTACTTTTCGATGGTGTTGAGATTTCAAGCGTATATTGGTTTGAGCCATGGCTTACAGGCCAGACTGCTGGAAGGTCCACGGATAACAGCAGCAGAACTGCAGTGCTTGATTCGTTAGGCAACTTTACTTCGTCTGATAGTTTCACATTAATGGCAGCTGATTATGGTGCAGGATTGCAGAGAAGACTGACGGTTGATTCTGATGGAAATGTTCGATTGTATAGTCGAGAAAAGCCCGGAGACAAATGGAGCGTTTCAGCGCAAGTCTTCTCCGATCCATGTAAAATTCACGGCGTTTGCGGAGTTAACAGCGTCTGCAGCTACGATCCATTTCATGGTAGAAAGTGCTCTTGTATTCCGGGTTACAAGATGAAAAATCATACTGATTGGTACTATGGTTGTGAGCCTCAATTTAATTACACTTGCAACAAAGGTGAGTCAACTTTCTTTAAACTTTCAAAGCTTGAGTTTTTCGGGTATGATTACGGTTACTTTGAAAACTACACCTATACGGATTGTGAGAATTTATGCTTGAAACTATGTAACTGCAAAGGGTTCCAATACTCTCGCATCCCTGACCGACCGATATTTAGCTGTTACCCCAAGACGGTATTGCTCAATGGATACCGGTCCCCTAGCTTTTTTGGGGACCTGTATTTGAGAGTGCCTAAAACCCATGTTTCGTATTATCAAAAGCCCCAGGAAGAGTATAGGTTAAGCTGCTTAAGCAGAGTTGTTGTTGGTCTAAATAGAAATTATGTGAAGAGCAGTGTGGCTAGTTCAGTGAAATTCCTGCTCTGGTTTGCCATTGGAGTTGGCGGGTTTGAGATCATTTGTATCCTTTTGGTTTGGGGATTGTTAAGCAATACTACTCGGCGAAATTCCAACGAGGATATGCAGGGATATGTTCTTGCTGGAACAGGGTTCAAAAGATTTAGCTATGCCGAGCTTAAGAAGGCGACACGGGGCTTTAGTGAAGAGATTGGAAGAGGGGCTGGAGGAATCGTGTACAAAGGCGTGCTGGCTGATCAAAGAGTTGCAGCGATTAAGCTTCTCAGCGAGGCTGATCAAGGAGAAGTTGAATTTCTAGGTGAAGCTAACACAATTGGGAGGCTGAACCATATGCACTTGATTGACATGTGGGGATATTGCTCAGAGAAAAAGCACAAGCTTCTCGTTTACGAGTACATGGAGCATGGTTCCTTGGCTGATAAGTTATCTTCCAATGTTCTTGATTGGGAGAAGAGGTTTGAGATTGCTGTCGGGACAGCGAAAGGCCTTGCTTATCTGCACGAAGAGTGTTTGGAGTGGGTTTTGCACTGTGATGTTAAGCCTCAAAACATTCTCTTGGACTCAGATTACCAACCAAAGGTTGCAGATTTTGGACTCTCCAAGCTAATCAACAGGGGCGGCCTTGAAAACTCGAGCATTTCGAGGATACGAGGAACGAGAGGATACATTGCACCGGAGTGGGTGTACAACCAGCCGATTACGTCGAAAGTGGATGTTTACAGCTATGGGATTGTTGTGTTGGAGATGTTGACCGGAAAGAACCCTACCATTGGCGTTGGTGGCGTTGAGGGTGAGCAGAGAGGACTGATTAATTGGGTGAGGGAGAAGGTTGATGGAAGTGGTGGGATTGGATCAAGGATGGGAGAGATCATAGACCGTTCAGTTGGAGAGAATTATGATGTGAGAAAGATGGAGATTCTGTTGGAAGTAGCTTTGCGTTGTGTAGAGGAAGAAAAAAATGCAAGACCTACCATGACCCAAGTGGTTGAAAAGCTCCTGCGGATTCACAAACACAGAGACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

807

Amino Acids

90.53

Weight (kDa)

6.99

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 87 - 174 9.2e-23 D-mannose binding lectin
S_locus_glycop PF00954 258 - 326 1e-07 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 525 - 797 3e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 526 - 795 1.8e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AccB7I CCANNNNNTGG 1 cut(s) 938
AccBSI CCGCTC 1 cut(s) 508
AccI GTMKAC 1 cut(s) 604
AccII CGCG 3 cut(s) 255, 257, 279
AciI CCGC 7 cut(s) 253, 257, 506, 903, 1422, 2013, 2395
AclWI GGATC 3 cut(s) 872, 923, 2254
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 5 cut(s) 888, 1126, 1391, 1483, 1685
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 4 cut(s) 995, 1613, 1790, 2081
AfiI CCNNNNNNNGG 6 cut(s) 124, 293, 938, 1210, 1972, 2165
AflII CTTAAG 2 cut(s) 1327, 1553
AflIII ACRYGT 1 cut(s) 1740
AgeI ACCGGT 2 cut(s) 286, 1230
AgsI TTSAA 8 cut(s) 630, 1062, 1100, 1138, 1531, 1685, 2021, 2384
AjnI CCWGG 1 cut(s) 1302
AleI CACNNNNGTG 1 cut(s) 2071
Alw21I GWGCWC 1 cut(s) 953
Alw26I GTCTC 3 cut(s) 535, 837, 2406
AlwI GGATC 3 cut(s) 872, 923, 2254
AlwNI CAGNNNCTG 1 cut(s) 918
Ama87I CYCGRG 1 cut(s) 2026
AoxI GGCC 4 cut(s) 352, 661, 1879, 2014
ApeKI GCWGC 6 cut(s) 395, 693, 758, 921, 1323, 1643
ApoI RAATTY 5 cut(s) 888, 1126, 1391, 1483, 1685
AseI ATTAAT 2 cut(s) 752, 2205
AsiGI ACCGGT 2 cut(s) 286, 1230
AspLEI GCGC 2 cut(s) 257, 865
AspS9I GGNCC 3 cut(s) 678, 1233, 1252
AsuC2I CCSGG 2 cut(s) 840, 963
AsuHPI GGTGA 9 cut(s) 144, 173, 214, 301, 502, 1049, 1706, 2198, 2224
AvaI CYCGRG 1 cut(s) 2026
AvaII GGWCC 3 cut(s) 678, 1233, 1252
BaeGI GKGCMC 1 cut(s) 32
BaeI ACNNNNGTAYC 2 cut(s) 985, 1018
BanI GGYRCC 1 cut(s) 29
BbsI GAAGAC 3 cut(s) 75, 793, 862
Bbv12I GWGCWC 1 cut(s) 953
BbvI GCAGC 6 cut(s) 407, 705, 770, 933, 1310, 1655
BccI CCATC 7 cut(s) 152, 262, 608, 800, 2222, 2248, 2299
BceAI ACGGC 1 cut(s) 910
BciT130I CCWGG 1 cut(s) 1304
BciVI GTATCC 4 cut(s) 1220, 1451, 2034, 2051
BclI TGATCA 2 cut(s) 1630, 1669
BcnI CCSGG 2 cut(s) 840, 963
BcoDI GTCTC 3 cut(s) 535, 837, 2406
BfaI CTAG 4 cut(s) 59, 1239, 1380, 1691
BfmI CTRYAG 2 cut(s) 702, 919
BfrI CTTAAG 2 cut(s) 1327, 1553
BfuI GTATCC 4 cut(s) 1220, 1451, 2034, 2051
BisI GCNGC 7 cut(s) 396, 694, 759, 922, 1324, 1644, 2014
BlsI GCNGC 7 cut(s) 397, 695, 760, 923, 1325, 1645, 2015
Bme1390I CCNGG 3 cut(s) 840, 963, 1304
Bme18I GGWCC 3 cut(s) 678, 1233, 1252
BmeT110I CYCGRG 1 cut(s) 2026
BmgT120I GGNCC 3 cut(s) 678, 1233, 1252
BmiI GGNNCC 5 cut(s) 31, 1160, 1235, 1253, 1804
BmrFI CCNGG 3 cut(s) 840, 963, 1304
BmsI GCATC 3 cut(s) 98, 337, 1182
BpiI GAAGAC 3 cut(s) 75, 793, 862
BpmI CTGGAG 2 cut(s) 395, 1620
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 2 cut(s) 840, 963
BsaI GGTCTC 1 cut(s) 2406
BsaJI CCNNGG 4 cut(s) 650, 681, 1302, 1806
BsaWI WCCGGW 5 cut(s) 154, 286, 1230, 2068, 2147
BsaXI ACNNNNNCTCC 8 cut(s) 1593, 1623, 1835, 1865, 2063, 2093, 2300, 2330
Bsc4I CCNNNNNNNGG 6 cut(s) 124, 293, 938, 1210, 1972, 2165
Bse118I RCCGGY 4 cut(s) 173, 286, 350, 1230
Bse1I ACTGG 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 2061
BseBI CCWGG 1 cut(s) 1304
BseDI CCNNGG 4 cut(s) 650, 681, 1302, 1806
BseGI GGATG 6 cut(s) 113, 163, 273, 1173, 2113, 2259
BseLI CCNNNNNNNGG 6 cut(s) 124, 293, 938, 1210, 1972, 2165
BseMI GCAATG 1 cut(s) 2061
BseMII CTCAG 4 cut(s) 432, 1672, 1770, 1971
BseNI ACTGG 1 cut(s) 355
BseRI GAGGAG 1 cut(s) 547
BseSI GKGCMC 1 cut(s) 32
BseXI GCAGC 6 cut(s) 407, 705, 770, 933, 1310, 1655
BsgI GTGCAG 2 cut(s) 792, 1877
Bsh1236I CGCG 3 cut(s) 255, 257, 279
Bsh1285I CGRYCG 1 cut(s) 1187
BshFI GGCC 4 cut(s) 354, 663, 1881, 2016
BshNI GGYRCC 1 cut(s) 29
BshTI ACCGGT 2 cut(s) 286, 1230
BsiEI CGRYCG 1 cut(s) 1187
BsiHKAI GWGCWC 1 cut(s) 953
BsiHKCI CYCGRG 1 cut(s) 2026
BsiSI CCGG 9 cut(s) 155, 174, 287, 351, 840, 962, 1231, 2069, 2148
BslFI GGGAC 5 cut(s) 54, 391, 1219, 1265, 1882
BslI CCNNNNNNNGG 6 cut(s) 124, 293, 938, 1210, 1972, 2165
BsmAI GTCTC 3 cut(s) 535, 837, 2406
BsmFI GGGAC 5 cut(s) 54, 391, 1219, 1265, 1882
BsnI GGCC 4 cut(s) 354, 663, 1881, 2016
Bso31I GGTCTC 1 cut(s) 2406
BsoBI CYCGRG 1 cut(s) 2026
Bsp1286I GDGCHC 2 cut(s) 32, 953
Bsp1407I TGTACA 2 cut(s) 1611, 2079
Bsp143I GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
Bsp19I CCATGG 1 cut(s) 650
Bsp68I TCGCGA 1 cut(s) 279
BspACI CCGC 7 cut(s) 253, 257, 506, 903, 1422, 2013, 2395
BspANI GGCC 4 cut(s) 354, 663, 1881, 2016
BspCNI CTCAG 4 cut(s) 431, 1671, 1769, 1970
BspFNI CGCG 3 cut(s) 255, 257, 279
BspLI GGNNCC 5 cut(s) 31, 1160, 1235, 1253, 1804
BspMAI CTGCAG 2 cut(s) 706, 923
BspPI GGATC 3 cut(s) 872, 923, 2254
BspQI GCTCTTC 1 cut(s) 1361
BspT107I GGYRCC 1 cut(s) 29
BspTI CTTAAG 2 cut(s) 1327, 1553
BspTNI GGTCTC 1 cut(s) 2406
BsrBI CCGCTC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 2061
BsrFI RCCGGY 4 cut(s) 173, 286, 350, 1230
BsrGI TGTACA 2 cut(s) 1611, 2079
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 4 cut(s) 173, 286, 350, 1230
BssECI CCNNGG 4 cut(s) 650, 681, 1302, 1806
BssMI GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
BssT1I CCWWGG 2 cut(s) 650, 1806
Bst2UI CCWGG 1 cut(s) 1304
Bst4CI ACNGT 5 cut(s) 796, 1091, 1213, 1925, 2273
Bst6I CTCTTC 6 cut(s) 1302, 1361, 1575, 1585, 1841, 1893
BstAFI CTTAAG 2 cut(s) 1327, 1553
BstAUI TGTACA 2 cut(s) 1611, 2079
BstC8I GCNNGC 4 cut(s) 175, 352, 1622, 1626
BstDEI CTNAG 4 cut(s) 418, 1658, 1756, 1957
BstDSI CCRYGG 2 cut(s) 650, 681
BstF5I GGATG 6 cut(s) 113, 163, 273, 1173, 2113, 2259
BstFNI CGCG 3 cut(s) 255, 257, 279
BstHHI GCGC 2 cut(s) 257, 865
BstKTI GATC 8 cut(s) 448, 880, 931, 1434, 1633, 1672, 2249, 2265
BstMAI GTCTC 3 cut(s) 535, 837, 2406
BstMBI GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
BstMCI CGRYCG 1 cut(s) 1187
BstMWI GCNNNNNNNGC 3 cut(s) 921, 1329, 2175
BstNI CCWGG 1 cut(s) 1304
BstNSI RCATGY 1 cut(s) 1744
BstSCI CCNGG 3 cut(s) 838, 961, 1302
BstSFI CTRYAG 2 cut(s) 702, 919
BstSLI GKGCMC 1 cut(s) 32
BstUI CGCG 3 cut(s) 255, 257, 279
BstV1I GCAGC 6 cut(s) 407, 705, 770, 933, 1310, 1655
BstV2I GAAGAC 3 cut(s) 75, 793, 862
BstXI CCANNNNNNTGG 2 cut(s) 326, 671
BsuI GTATCC 4 cut(s) 1220, 1451, 2034, 2051
BsuRI GGCC 4 cut(s) 354, 663, 1881, 2016
BtgI CCRYGG 2 cut(s) 650, 681
BtsCI GGATG 6 cut(s) 113, 163, 273, 1173, 2113, 2259
BtsI GCAGTG 3 cut(s) 168, 711, 1378
BtsIMutI CAGTG 5 cut(s) 168, 711, 1378, 1392, 1921
BtuMI TCGCGA 1 cut(s) 279
Cac8I GCNNGC 4 cut(s) 175, 352, 1622, 1626
CaiI CAGNNNCTG 1 cut(s) 918
CfoI GCGC 2 cut(s) 257, 865
Cfr10I RCCGGY 4 cut(s) 173, 286, 350, 1230
Cfr13I GGNCC 3 cut(s) 678, 1233, 1252
CseI GACGC 2 cut(s) 590, 904
Csp6I GTAC 4 cut(s) 994, 1612, 1789, 2080
CspAI ACCGGT 2 cut(s) 286, 1230
CviAII CATG 9 cut(s) 25, 651, 882, 938, 1280, 1741, 1792, 1799, 2368
CviQI GTAC 4 cut(s) 994, 1612, 1789, 2080
DdeI CTNAG 4 cut(s) 418, 1658, 1756, 1957
DpnI GATC 8 cut(s) 447, 879, 930, 1433, 1632, 1671, 2248, 2264
DpnII GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
DraI TTTAAA 1 cut(s) 1054
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 6 cut(s) 1302, 1361, 1575, 1585, 1841, 1893
EarI CTCTTC 6 cut(s) 1302, 1361, 1575, 1585, 1841, 1893
Eco130I CCWWGG 2 cut(s) 650, 1806
Eco147I AGGCCT 1 cut(s) 1881
Eco31I GGTCTC 1 cut(s) 2406
Eco47I GGWCC 3 cut(s) 678, 1233, 1252
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 2026
EcoO109I RGGNCCY 1 cut(s) 1252
EcoRII CCWGG 1 cut(s) 1302
EcoT14I CCWWGG 2 cut(s) 650, 1806
ErhI CCWWGG 2 cut(s) 650, 1806
FaeI CATG 9 cut(s) 28, 654, 885, 941, 1283, 1744, 1795, 1802, 2371
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 5 cut(s) 54, 391, 1219, 1265, 1882
FatI CATG 9 cut(s) 24, 650, 881, 937, 1279, 1740, 1791, 1798, 2367
FauI CCCGC 1 cut(s) 1415
FauNDI CATATG 1 cut(s) 1725
FbaI TGATCA 2 cut(s) 1630, 1669
FblI GTMKAC 1 cut(s) 604
Fnu4HI GCNGC 7 cut(s) 396, 694, 759, 922, 1324, 1644, 2014
FokI GGATG 6 cut(s) 100, 170, 280, 1160, 2120, 2266
Fsp4HI GCNGC 7 cut(s) 396, 694, 759, 922, 1324, 1644, 2014
FspBI CTAG 4 cut(s) 59, 1239, 1380, 1691
GlaI GCGC 2 cut(s) 256, 864
GluI GCNGC 7 cut(s) 396, 694, 759, 922, 1324, 1644, 2014
GsuI CTGGAG 2 cut(s) 395, 1620
HaeIII GGCC 4 cut(s) 354, 663, 1881, 2016
HapII CCGG 9 cut(s) 155, 174, 287, 351, 840, 962, 1231, 2069, 2148
HgaI GACGC 2 cut(s) 590, 904
HhaI GCGC 2 cut(s) 257, 865
Hin1II CATG 9 cut(s) 28, 654, 885, 941, 1283, 1744, 1795, 1802, 2371
Hin6I GCGC 2 cut(s) 255, 863
HinP1I GCGC 2 cut(s) 255, 863
HincII GTYRAC 3 cut(s) 910, 1044, 2145
HindII GTYRAC 3 cut(s) 910, 1044, 2145
HindIII AAGCTT 4 cut(s) 572, 1064, 1652, 1772
HinfI GANTC 9 cut(s) 274, 713, 800, 1040, 1605, 1955, 1989, 2311, 2398
HpaI GTTAAC 1 cut(s) 910
HpaII CCGG 9 cut(s) 155, 174, 287, 351, 840, 962, 1231, 2069, 2148
HphI GGTGA 9 cut(s) 144, 173, 214, 301, 502, 1049, 1706, 2198, 2224
Hpy188I TCNGA 6 cut(s) 94, 739, 805, 877, 1759, 1960
Hpy188III TCNNGA 5 cut(s) 278, 412, 830, 1835, 1867
Hpy99I CGWCG 1 cut(s) 2101
HpyAV CCTTC 7 cut(s) 83, 87, 240, 500, 669, 1552, 2215
HpyCH4III ACNGT 5 cut(s) 796, 1091, 1213, 1925, 2273
HpyCH4IV ACGT 2 cut(s) 383, 2096
HpyF10VI GCNNNNNNNGC 3 cut(s) 921, 1329, 2175
HpyF3I CTNAG 4 cut(s) 418, 1658, 1756, 1957
HpySE526I ACGT 2 cut(s) 383, 2096
Hsp92II CATG 9 cut(s) 28, 654, 885, 941, 1283, 1744, 1795, 1802, 2371
HspAI GCGC 2 cut(s) 255, 863
KroI GCCGGC 2 cut(s) 173, 350
KroNI GCCGGC 2 cut(s) 175, 352
Ksp22I TGATCA 2 cut(s) 1630, 1669
KspAI GTTAAC 1 cut(s) 910
Kzo9I GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
LguI GCTCTTC 1 cut(s) 1361
LmnI GCTCC 3 cut(s) 852, 1795, 2394
Lsp1109I GCAGC 6 cut(s) 407, 705, 770, 933, 1310, 1655
LweI GCATC 3 cut(s) 98, 337, 1182
MaeI CTAG 4 cut(s) 59, 1239, 1380, 1691
MaeII ACGT 2 cut(s) 383, 2096
MaeIII GTNAC 6 cut(s) 20, 384, 965, 1091, 1145, 1199
MalI GATC 8 cut(s) 447, 879, 930, 1433, 1632, 1671, 2248, 2264
MbiI CCGCTC 1 cut(s) 508
MboI GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
MfeI CAATTG 1 cut(s) 1707
MhlI GDGCHC 2 cut(s) 32, 953
MlyI GAGTC 3 cut(s) 1049, 1949, 1983
MmeI TCCRAC 5 cut(s) 503, 1512, 2115, 2260, 2298
MroNI GCCGGC 2 cut(s) 173, 350
MslI CAYNNNNRTG 3 cut(s) 752, 1926, 2071
MspA1I CMGCKG 1 cut(s) 761
MspCI CTTAAG 2 cut(s) 1327, 1553
MspI CCGG 9 cut(s) 155, 174, 287, 351, 840, 962, 1231, 2069, 2148
MspR9I CCNGG 3 cut(s) 840, 963, 1304
MunI CAATTG 1 cut(s) 1707
MvaI CCWGG 1 cut(s) 1304
MvnI CGCG 3 cut(s) 255, 257, 279
MwoI GCNNNNNNNGC 3 cut(s) 921, 1329, 2175
NaeI GCCGGC 2 cut(s) 175, 352
NciI CCSGG 2 cut(s) 840, 963
NcoI CCATGG 1 cut(s) 650
NdeI CATATG 1 cut(s) 1725
NdeII GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
NgoMIV GCCGGC 2 cut(s) 173, 350
NlaIII CATG 9 cut(s) 28, 654, 885, 941, 1283, 1744, 1795, 1802, 2371
NlaIV GGNNCC 5 cut(s) 31, 1160, 1235, 1253, 1804
NmeAIII GCCGAG 3 cut(s) 371, 1456, 1573
NmuCI GTSAC 2 cut(s) 20, 384
NruI TCGCGA 1 cut(s) 279
NspI RCATGY 1 cut(s) 1744
OliI CACNNNNGTG 1 cut(s) 2071
PaeR7I CTCGAG 1 cut(s) 2026
PceI AGGCCT 1 cut(s) 1881
PciI ACATGT 1 cut(s) 1740
PciSI GCTCTTC 1 cut(s) 1361
PcsI WCGNNNNNNNCGW 1 cut(s) 2033
PdiI GCCGGC 2 cut(s) 175, 352
PfeI GAWTC 6 cut(s) 274, 713, 800, 1605, 2311, 2398
PflMI CCANNNNNTGG 1 cut(s) 938
PinAI ACCGGT 2 cut(s) 286, 1230
PkrI GCNGC 7 cut(s) 397, 695, 760, 923, 1325, 1645, 2015
PleI GAGTC 3 cut(s) 1048, 1949, 1983
PpsI GAGTC 3 cut(s) 1048, 1949, 1983
PpuMI RGGWCCY 1 cut(s) 1252
PscI ACATGT 1 cut(s) 1740
PshBI ATTAAT 2 cut(s) 752, 2205
Psp5II RGGWCCY 1 cut(s) 1252
Psp6I CCWGG 1 cut(s) 1302
PspGI CCWGG 1 cut(s) 1302
PspN4I GGNNCC 5 cut(s) 31, 1160, 1235, 1253, 1804
PspPI GGNCC 3 cut(s) 678, 1233, 1252
PspPPI RGGWCCY 1 cut(s) 1252
PspXI VCTCGAGB 1 cut(s) 2026
PsrI GAACNNNNNNTAC 2 cut(s) 284, 316
PstI CTGCAG 2 cut(s) 706, 923
PstNI CAGNNNCTG 1 cut(s) 918
PvuII CAGCTG 1 cut(s) 761
RruI TCGCGA 1 cut(s) 279
RsaI GTAC 4 cut(s) 995, 1613, 1790, 2081
RsaNI GTAC 4 cut(s) 994, 1612, 1789, 2080
RseI CAYNNNNRTG 3 cut(s) 752, 1926, 2071
SapI GCTCTTC 1 cut(s) 1361
SatI GCNGC 7 cut(s) 396, 694, 759, 922, 1324, 1644, 2014
Sau3AI GATC 8 cut(s) 445, 877, 928, 1431, 1630, 1669, 2246, 2262
Sau96I GGNCC 3 cut(s) 678, 1233, 1252
SchI GAGTC 3 cut(s) 1049, 1949, 1983
ScrFI CCNGG 3 cut(s) 840, 963, 1304
SduI GDGCHC 2 cut(s) 32, 953
SfaNI GCATC 3 cut(s) 98, 337, 1182
SfcI CTRYAG 2 cut(s) 702, 919
Sfr274I CTCGAG 1 cut(s) 2026
SinI GGWCC 3 cut(s) 678, 1233, 1252
SlaI CTCGAG 1 cut(s) 2026
SmiMI CAYNNNNRTG 3 cut(s) 752, 1926, 2071
SmlI CTYRAG 4 cut(s) 1067, 1327, 1553, 2026
SmoI CTYRAG 4 cut(s) 1067, 1327, 1553, 2026
SseBI AGGCCT 1 cut(s) 1881
SsiI CCGC 7 cut(s) 253, 257, 506, 903, 1422, 2013, 2395
SspMI CTAG 4 cut(s) 59, 1239, 1380, 1691
StuI AGGCCT 1 cut(s) 1881
StyD4I CCNGG 3 cut(s) 838, 961, 1302
StyI CCWWGG 2 cut(s) 650, 1806
TaaI ACNGT 5 cut(s) 796, 1091, 1213, 1925, 2273
TaiI ACGT 2 cut(s) 386, 2099
TaqI TCGA 6 cut(s) 612, 817, 829, 2027, 2036, 2099
TaqII GACCGA 2 cut(s) 1197, 1201
TatI WGTACW 3 cut(s) 1611, 1788, 2079
TauI GCSGC 1 cut(s) 2016
TfiI GAWTC 6 cut(s) 274, 713, 800, 1605, 2311, 2398
TscAI CASTG 5 cut(s) 175, 711, 1378, 1392, 1928
TseFI GTSAC 2 cut(s) 20, 384
TseI GCWGC 6 cut(s) 395, 693, 758, 921, 1323, 1643
Tsp45I GTSAC 2 cut(s) 20, 384
TspDTI ATGAA 3 cut(s) 4, 926, 989
TspGWI ACGGA 2 cut(s) 698, 1130
TspRI CASTG 5 cut(s) 175, 711, 1378, 1392, 1928
Van91I CCANNNNNTGG 1 cut(s) 938
Vha464I CTTAAG 2 cut(s) 1327, 1553
VpaK11BI GGWCC 3 cut(s) 678, 1233, 1252
VspI ATTAAT 2 cut(s) 752, 2205
XapI RAATTY 5 cut(s) 888, 1126, 1391, 1483, 1685
XceI RCATGY 1 cut(s) 1744
XcmI CCANNNNNNNNNTGG 1 cut(s) 2374
XhoI CTCGAG 1 cut(s) 2026
XmiI GTMKAC 1 cut(s) 604
XspI CTAG 4 cut(s) 59, 1239, 1380, 1691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.