MD12G1152000.v1.1

Receptor protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000166
Physical Location & Seq
Forward (+)
23182942 .. 23184109
1168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1152000.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGATCATACATATTAAGGTTCCAAAGGGATTTCTGAACCCTCATGCGCTCCCAACGAAGTTTGAAACACATGATTTGAACTGTTCAGGTGCACAAGTTGTTTTCAACAGCAACGATCCTGAAGCAGAGCGCAATAGAAACTGGTACATGAAATACCTAATTGGATTTGTAGGTTCTTTTGCAGTAATTGAAGCACTCTGTGTTGGTTTGACGTGGTGGTATCTCTTCCGAAAGCATGCTCATGAGGAGTTGTCCAATATAGCCGGTTACATGGCATTGGCCATGGACTTCAAACGCTTCACCTATGCAGAACTAACAAGAGCCACCGGTAACTTCAAACAGAAGATAGGGAAAGGGGGCTTCGGAACTGTCTATAAAGGGCTACTGGATGATGGAAGAGTTGTTGCGGTGAAGAGACTAGAAGGCGTCTTGCAAGAAAGGCAGAAACGGATCCTGGAGAAAATCTTGTTTTCGGATGTGGAATTTGGATTGGAGAAGCGATACAATATTGCACTAGGCACTGCAAATGGTTTGTCATATTTACACGAGTGCCTGGAATGGGTGCTTCACTGTGATGTGAAGCCTCAAAACATACTGTTAGCCGATGATTTGGAACCTCGGGTGGCAGATTTTGGCATGGCAAAGCTATTCAAAGATATCCACGGCATCAGAGACTGTCATGGGATTGGCTTTTCACGAGTGCGTGGCACCAGAGGTTATTTGGCTCCAGAGTGGATGATGAACCTGAAAATTGATGCGAAGGCGGTCGTGTACAGCTATGGGGTTGTGCTGCTGGAACTACTGAGTGGAAGAAGTGCTTCTAGCTTCCTTTCAACCGGTGGCCAATACAATGAATACAACCACTTGGTACATTGGGTGACTGAAATGATCGGAGAAGAAGGACTTGAGAAAGTAATCGACCCTCGGCTACACCATGAGTTCAACACGAAGCTCAAACGATTGATGAAAGTCGCTATGTCGTGCGTACAAGAAGATCGCAAAGCAAGGCCTGCCATGAGTAAGGTTGTGGAGCTTCTCCTTGAAAATGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_1 PAN_2 Pkinase S_locus_glycop

Protein Analysis

352

Amino Acids

39.97

Weight (kDa)

8.11

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 152 - 343 1.8e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 161 - 346 1.1e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000166)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g33870 FvH4_5g04230 FvH4_5g04261 FvH4_5g04270 FvH4_5g04300 FvH4_5g04310 FvH4_5g04350 FvH4_6g12870 FvH4_6g12880 FvH4_6g12881 FvH4_6g12890 FvH4_6g12920 FvH4_6g12930
malus_domestica MD04G1137000.v1.1 MD04G1137200.v1.1 MD04G1137300.v1.1 MD12G1151200.v1.1 MD12G1151300.v1.1 MD12G1151400.v1.1 MD12G1151500.v1.1 MD12G1151600.v1.1 MD12G1152000.v1.1 MD15G1076100.v1.1 MD15G1076300.v1.1 MD15G1076400.v1.1
prunus_persica Prupe.1G429600_v2.0.a1 Prupe.1G429700_v2.0.a1 Prupe.5G116500_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.5G116800_v2.0.a1 Prupe.6G263000_v2.0.a1 Prupe.6G263100_v2.0.a1 Prupe.6G263200_v2.0.a1 Prupe.6G263300_v2.0.a1
pyrus_communis pycom04g12430 pycom04g12520 pycom04g12530 pycom04g12550 pycom12g14480 pycom12g14510 pycom15g07110 pycom15g07120
rosa_chinensis RchiOBHm_Chr3g0464611 RchiOBHm_Chr3g0464901 RchiOBHm_Chr3g0464911 RchiOBHm_Chr3g0464941 RchiOBHm_Chr3g0464971 RchiOBHm_Chr3g0464981 RchiOBHm_Chr3g0464991 RchiOBHm_Chr3g0465001 RchiOBHm_Chr3g0465011 RchiOBHm_Chr6g0308531 RchiOBHm_Chr6g0308541 RchiOBHm_Chr6g0308551 RchiOBHm_Chr6g0308581 RchiOBHm_Chr6g0308601 RchiOBHm_Chr6g0308641 RchiOBHm_Chr6g0308651 RchiOBHm_Chr6g0308661 RchiOBHm_Chr6g0308671 RchiOBHm_Chr7g0195221
rosa_laevigata RLG00000001258 RLG00000001261 RLG00000001262 RLG00000001263 RLG00000004137 RLG00000010630 RLG00000010631 RLG00000010633 RLG00000010634 RLG00000024661 RLG00000024662 RLG00000024663 RLG00000024664 RLG00000024665 RLG00000024666 RLG00000024681
rosa_multiflora Rmu_co8160990.1_g000001 Rmu_co8270195.1_g000001 Rmu_co8301123.1_g000001 Rmu_co8390765.1_g000001 Rmu_co8418605.1_g000001 Rmu_sc0000871.1_g000001 Rmu_sc0000871.1_g000007 Rmu_sc0000871.1_g000008 Rmu_sc0001161.1_g000016 Rmu_sc0001161.1_g000017 Rmu_sc0001161.1_g000018 Rmu_sc0002031.1_g000009 Rmu_sc0002381.1_g000010 Rmu_sc0004362.1_g000025 Rmu_sc0004362.1_g000026 Rmu_sc0013106.1_g000001 Rmu_sc0014534.1_g000001 Rmu_sc0014550.1_g000011 Rmu_sc0018858.1_g000002 Rmu_sc0019815.1_g000004 Rmu_sc0029154.1_g000002
rosa_roxburghii Rroxscaffold_3G00260650 Rroxscaffold_6G00415330 Rroxscaffold_6G00415340 Rroxscaffold_6G00415350 Rroxscaffold_6G00415360 Rroxscaffold_6G00415530 Rroxscaffold_7G00160020 Rroxscaffold_7G00160030 Rroxscaffold_7G00160040
rosa_rugosa Rorug03G0073200.1 Rorug03G0074600 Rorug03G0074600 Rorug03G0074700 Rorug03G0074800 Rorug06G0367700 Rorug07G0021800 Rorug07G0183500 Rorug07G0183600 Rorug07G0183700 Rorug07G0183800
rosa_samantha Rh3BG135400 Rh3BG137500 Rh3BG137700 Rh3BG137800 Rh3BG137900 Rh3BG138000 Rh3BG138100 Rh3BG138200 Rh3BG138300 Rh3BG284900 Rh3CG138100 Rh3CG140100 Rh3CG140300 Rh3CG140400 Rh3CG140500 Rh3CG140600 Rh3CG140700 Rh3CG148700 Rh3CG148800 Rh3CG148900 Rh3CG149000 Rh3DG137000 Rh3DG138900 Rh3DG139100 Rh3DG139200 Rh3DG139300 Rh3DG139400 Rh3DG139500 Rh3DG139600 Rh3DG139700 Rh3DG139800 Rh6AG477500 Rh6AG477800 Rh6AG478300 Rh6AG478500 Rh6AG478600 Rh6BG487800 Rh6CG492800 Rh6DG479000 Rh6DG479100 Rh7AG149000 Rh7BG150200 Rh7CG153800 Rh7DG150100
rosa_wichuraiana Rw3G011090 Rw3G011240 Rw3G011250 Rw3G011260 Rw3G011270 Rw3G011280 Rw3G011290 Rw3G011300 Rw3G011310 Rw6G041670 Rw6G041680 Rw6G041700 Rw6G041710 Rw7G012730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 707
AciI CCGC 2 cut(s) 407, 764
AclWI GGATC 3 cut(s) 110, 445, 458
AcoI YGGCCR 2 cut(s) 279, 841
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 426
AdeI CACNNNGTG 1 cut(s) 200
AfaI GTAC 4 cut(s) 146, 773, 870, 987
AfiI CCNNNNNNNGG 1 cut(s) 559
AgeI ACCGGT 2 cut(s) 326, 836
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 453, 552
AluBI AGCT 5 cut(s) 646, 777, 825, 952, 1033
AluI AGCT 5 cut(s) 646, 777, 825, 952, 1033
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 2 cut(s) 409, 666
Alw44I GTGCAC 1 cut(s) 90
AlwI GGATC 3 cut(s) 110, 445, 458
AlwNI CAGNNNCTG 1 cut(s) 675
Ama87I CYCGRG 1 cut(s) 618
AoxI GGCC 3 cut(s) 279, 841, 1007
ApaLI GTGCAC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 790
ApoI RAATTY 1 cut(s) 482
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
AsiGI ACCGGT 2 cut(s) 326, 836
Asp700I GAANNNNTTC 1 cut(s) 817
AspLEI GCGC 2 cut(s) 49, 132
AsuHPI GGTGA 3 cut(s) 292, 421, 889
AvaI CYCGRG 1 cut(s) 618
BaeGI GKGCMC 1 cut(s) 94
BalI TGGCCA 2 cut(s) 281, 843
BamHI GGATCC 1 cut(s) 450
BanI GGYRCC 1 cut(s) 707
BauI CACGAG 2 cut(s) 545, 696
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 777
BccI CCATC 1 cut(s) 386
BceAI ACGGC 1 cut(s) 679
BciT130I CCWGG 2 cut(s) 455, 554
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 2 cut(s) 409, 666
BfaI CTAG 3 cut(s) 419, 515, 822
BisI GCNGC 1 cut(s) 791
BlsI GCNGC 1 cut(s) 792
Bme1390I CCNGG 2 cut(s) 455, 554
BmeT110I CYCGRG 1 cut(s) 618
BmgBI CACGTC 1 cut(s) 213
BmiI GGNNCC 5 cut(s) 21, 452, 615, 709, 726
BmrFI CCNGG 2 cut(s) 455, 554
BmsI GCATC 2 cut(s) 675, 745
BpmI CTGGAG 2 cut(s) 476, 711
BpuEI CTTGAG 1 cut(s) 926
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 4 cut(s) 282, 617, 661, 923
BsaWI WCCGGW 2 cut(s) 326, 836
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse118I RCCGGY 3 cut(s) 263, 326, 836
Bse1I ACTGG 2 cut(s) 146, 390
BseBI CCWGG 2 cut(s) 455, 554
BseDI CCNNGG 4 cut(s) 282, 617, 661, 923
BseGI GGATG 3 cut(s) 394, 481, 741
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMII CTCAG 1 cut(s) 794
BseNI ACTGG 2 cut(s) 146, 390
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 1 cut(s) 777
Bsh1285I CGRYCG 1 cut(s) 768
BshFI GGCC 3 cut(s) 281, 843, 1009
BshNI GGYRCC 1 cut(s) 707
BshTI ACCGGT 2 cut(s) 326, 836
BsiEI CGRYCG 1 cut(s) 768
BsiHKAI GWGCWC 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 3 cut(s) 264, 327, 837
BslI CCNNNNNNNGG 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 409, 666
BsnI GGCC 3 cut(s) 281, 843, 1009
BsoBI CYCGRG 1 cut(s) 618
Bsp1286I GDGCHC 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 771
Bsp143I GATC 5 cut(s) 3, 115, 450, 888, 994
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 2 cut(s) 407, 764
BspANI GGCC 3 cut(s) 281, 843, 1009
BspCNI CTCAG 1 cut(s) 795
BspHI TCATGA 1 cut(s) 241
BspLI GGNNCC 5 cut(s) 21, 452, 615, 709, 726
BspPI GGATC 3 cut(s) 110, 445, 458
BspT107I GGYRCC 1 cut(s) 707
BsrFI RCCGGY 3 cut(s) 263, 326, 836
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 2 cut(s) 146, 390
BssAI RCCGGY 3 cut(s) 263, 326, 836
BssECI CCNNGG 4 cut(s) 282, 617, 661, 923
BssMI GATC 5 cut(s) 3, 115, 450, 888, 994
BssNI GRCGYC 1 cut(s) 426
BssSI CACGAG 2 cut(s) 545, 696
BssT1I CCWWGG 1 cut(s) 282
Bst2BI CACGAG 2 cut(s) 545, 696
Bst2UI CCWGG 2 cut(s) 455, 554
Bst4CI ACNGT 5 cut(s) 83, 370, 572, 597, 677
Bst6I CTCTTC 3 cut(s) 230, 391, 407
BstACI GRCGYC 1 cut(s) 426
BstAPI GCANNNNNTGC 1 cut(s) 1010
BstAUI TGTACA 1 cut(s) 771
BstC8I GCNNGC 2 cut(s) 237, 1011
BstDEI CTNAG 1 cut(s) 803
BstDSI CCRYGG 2 cut(s) 282, 661
BstF5I GGATG 3 cut(s) 394, 481, 741
BstHHI GCGC 2 cut(s) 49, 132
BstKTI GATC 5 cut(s) 6, 118, 453, 891, 997
BstMAI GTCTC 2 cut(s) 409, 666
BstMBI GATC 5 cut(s) 3, 115, 450, 888, 994
BstMCI CGRYCG 1 cut(s) 768
BstMWI GCNNNNNNNGC 2 cut(s) 439, 1010
BstNI CCWGG 2 cut(s) 455, 554
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 453, 552
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 777
BstX2I RGATCY 1 cut(s) 450
BstYI RGATCY 1 cut(s) 450
BsuRI GGCC 3 cut(s) 281, 843, 1009
BtgI CCRYGG 2 cut(s) 282, 661
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 3 cut(s) 394, 481, 741
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 519, 568
Cac8I GCNNGC 2 cut(s) 237, 1011
CaiI CAGNNNCTG 1 cut(s) 675
CciI TCATGA 1 cut(s) 241
CfoI GCGC 2 cut(s) 49, 132
Cfr10I RCCGGY 3 cut(s) 263, 326, 836
CseI GACGC 1 cut(s) 415
Csp6I GTAC 4 cut(s) 145, 772, 869, 986
CspAI ACCGGT 2 cut(s) 326, 836
CviQI GTAC 4 cut(s) 145, 772, 869, 986
DdeI CTNAG 1 cut(s) 803
DpnI GATC 5 cut(s) 5, 117, 452, 890, 996
DpnII GATC 5 cut(s) 3, 115, 450, 888, 994
DraIII CACNNNGTG 1 cut(s) 200
EaeI YGGCCR 2 cut(s) 279, 841
Eam1104I CTCTTC 3 cut(s) 230, 391, 407
EarI CTCTTC 3 cut(s) 230, 391, 407
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 1009
Eco32I GATATC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 618
EcoRII CCWGG 2 cut(s) 453, 552
EcoRV GATATC 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FalI AAGNNNNNCTT 4 cut(s) 802, 834, 888, 920
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 791
FokI GGATG 3 cut(s) 401, 488, 748
Fsp4HI GCNGC 1 cut(s) 791
FspBI CTAG 3 cut(s) 419, 515, 822
GlaI GCGC 2 cut(s) 48, 131
GluI GCNGC 1 cut(s) 791
GsuI CTGGAG 2 cut(s) 476, 711
HaeIII GGCC 3 cut(s) 281, 843, 1009
HapII CCGG 3 cut(s) 264, 327, 837
HgaI GACGC 1 cut(s) 415
HhaI GCGC 2 cut(s) 49, 132
Hin1I GRCGYC 1 cut(s) 426
Hin6I GCGC 2 cut(s) 47, 130
HinP1I GCGC 2 cut(s) 47, 130
HpaII CCGG 3 cut(s) 264, 327, 837
HphI GGTGA 3 cut(s) 292, 421, 889
Hpy166II GTNNAC 2 cut(s) 92, 772
Hpy188I TCNGA 6 cut(s) 36, 230, 365, 475, 671, 893
Hpy188III TCNNGA 4 cut(s) 119, 242, 696, 728
Hpy8I GTNNAC 2 cut(s) 92, 772
HpyAV CCTTC 3 cut(s) 416, 754, 893
HpyCH4III ACNGT 5 cut(s) 83, 370, 572, 597, 677
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 6 cut(s) 92, 182, 308, 433, 512, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 439, 1010
HpyF3I CTNAG 1 cut(s) 803
HpySE526I ACGT 1 cut(s) 212
Hsp92I GRCGYC 1 cut(s) 426
HspAI GCGC 2 cut(s) 47, 130
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 5 cut(s) 3, 115, 450, 888, 994
LmnI GCTCC 3 cut(s) 54, 730, 1030
Lsp1109I GCAGC 1 cut(s) 777
LweI GCATC 2 cut(s) 675, 745
MaeI CTAG 3 cut(s) 419, 515, 822
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 3 cut(s) 266, 329, 877
MalI GATC 5 cut(s) 5, 117, 452, 890, 996
MboI GATC 5 cut(s) 3, 115, 450, 888, 994
MboII GAAGA 7 cut(s) 217, 355, 408, 424, 822, 908, 1004
MflI RGATCY 1 cut(s) 450
MhlI GDGCHC 1 cut(s) 94
MlsI TGGCCA 2 cut(s) 281, 843
MluCI AATT 4 cut(s) 159, 186, 482, 750
MluNI TGGCCA 2 cut(s) 281, 843
MnlI CCTC 6 cut(s) 51, 238, 594, 627, 707, 933
Mox20I TGGCCA 2 cut(s) 281, 843
MroXI GAANNNNTTC 1 cut(s) 817
MscI TGGCCA 2 cut(s) 281, 843
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 2 cut(s) 240, 573
Msp20I TGGCCA 2 cut(s) 281, 843
MspI CCGG 3 cut(s) 264, 327, 837
MspR9I CCNGG 2 cut(s) 455, 554
MvaI CCWGG 2 cut(s) 455, 554
MwoI GCNNNNNNNGC 2 cut(s) 439, 1010
NcoI CCATGG 1 cut(s) 282
NdeII GATC 5 cut(s) 3, 115, 450, 888, 994
NlaIV GGNNCC 5 cut(s) 21, 452, 615, 709, 726
NmeAIII GCCGAG 1 cut(s) 904
NmuCI GTSAC 1 cut(s) 877
NspI RCATGY 1 cut(s) 239
PaeI GCATGC 1 cut(s) 239
PagI TCATGA 1 cut(s) 241
PceI AGGCCT 1 cut(s) 1009
PdmI GAANNNNTTC 1 cut(s) 817
PfoI TCCNGGA 1 cut(s) 453
PinAI ACCGGT 2 cut(s) 326, 836
PkrI GCNGC 1 cut(s) 792
Psp6I CCWGG 2 cut(s) 453, 552
PspGI CCWGG 2 cut(s) 453, 552
PspN4I GGNNCC 5 cut(s) 21, 452, 615, 709, 726
PstNI CAGNNNCTG 1 cut(s) 675
PsuI RGATCY 1 cut(s) 450
RsaI GTAC 4 cut(s) 146, 773, 870, 987
RsaNI GTAC 4 cut(s) 145, 772, 869, 986
RseI CAYNNNNRTG 2 cut(s) 240, 573
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 1 cut(s) 791
Sau3AI GATC 5 cut(s) 3, 115, 450, 888, 994
ScrFI CCNGG 2 cut(s) 455, 554
SduI GDGCHC 1 cut(s) 94
SfaNI GCATC 2 cut(s) 675, 745
SmiMI CAYNNNNRTG 2 cut(s) 240, 573
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
SphI GCATGC 1 cut(s) 239
Sse9I AATT 4 cut(s) 159, 186, 482, 750
SseBI AGGCCT 1 cut(s) 1009
SsiI CCGC 2 cut(s) 407, 764
SspI AATATT 1 cut(s) 508
SspMI CTAG 3 cut(s) 419, 515, 822
StuI AGGCCT 1 cut(s) 1009
StyD4I CCNGG 2 cut(s) 453, 552
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 5 cut(s) 83, 370, 572, 597, 677
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 918
TasI AATT 4 cut(s) 159, 186, 482, 750
TatI WGTACW 1 cut(s) 771
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 2 cut(s) 526, 575
TseFI GTSAC 1 cut(s) 877
TseI GCWGC 1 cut(s) 790
Tsp45I GTSAC 1 cut(s) 877
TspDTI ATGAA 4 cut(s) 164, 755, 867, 980
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 2 cut(s) 526, 575
VneI GTGCAC 1 cut(s) 90
XapI RAATTY 1 cut(s) 482
XceI RCATGY 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 817
XspI CTAG 3 cut(s) 419, 515, 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.