AT4G10400

microtubule motor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000140
Physical Location & Seq
Reverse (-)
6446088 .. 6448136
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10400.3

Sequence Viewer

Length: 1230 bp
ATGGACAGAATCAGTGGGTTACCTGATGAAGTACTGGTTAAGATACTGTCGTTTGTTCCGACTAAAGTTGCTGTGTCAACGAGCATTTTGTCGAAACGATGGGAGTTTCTTTGGATGTGGTTGACTAAACTTAAGTTTGGTAGTAAACGTTATTCAGAGTCTGAATTCAAGAGGTTACAGTGTTTTCTTGATAGGAATCTGCCATTACATAGAGCTCCCGTTATAGAAAGTTTCCGTCTTGTGTTAAGTGATTCGCATTTTAAACCTGAGGATATCAGAATGTGGGTTGTAGTAGCAGTTTCTCGCTACATACGTGAGCTGAAGATTTATAGTTCTCACTATGGGGAGAAGCAAAACATATTGCCAAGTAGCTTATATACCTGCAAATCGCTCGTGATCTTGAAACTCGATGGCGGGGTTCTCTTGGATGTTCCTCGAATGGTTTGTCTTCCCTCTTTGAAAACTTTGGAACTGAAAGGTGTGAGATACTTCAAGCAAGGATCTCTTCAGCGGCTTCTATGTAATTGCCCTGTTCTTGAAGATCTAGTAGTGAATCTATCTCATCATGACAATATGGGAAAGCTTACTGTTATTGTCCCATCTTTGCAACGTTTATCACTCTCTACACCTTCTAGTCGTGAGTTTGTCATAGATACTCCTTCTTTACTGTCTTTCCAACTTGTGGATCGTAATGATAATAGTCACACCTTTTTGATTGAGAATATGCCTAAGCTGAGGGAAGCATATATCAATGTTCCATTCGCTGATATCAAGAGTCTCATCGGATCAATCACATCTGTCAAGCGGCTTGCAATATCCTCTGAGGTTGGTTACGGTGAAGGTTTTATCTTCAACCACCTTGAGGAACTAACGCTATGGAACAAGTATTCTTCGAATCTCCTCGTCTGGTTCCTCAAAAACTCTCCTAACTTACGAGAACTAATGCTCGTCTCCGAAACGGATGATCATGAGAATCTTGGTATGCTTTCCTGGAATCAACCGAGTATTGTTCCTGAATGCATGTTGTCGAGTCTACAAAAATTCACTTGGTTTAAATACTTAGGAAGACCACAAGACAGAGATATTGCGGTTTACATCTTGAAAAACGCTTGCCGCTTAAGGACTGCAACAATCAAATCTGATACACGTTTGTTTACAAAACTCGAGATGATAACGGAGTTGAGACTTTCTTCCCAAGCTTCATCAACATGCGAACTCAACTTTTCTTGA

Protein Analysis

409

Amino Acids

47.05

Weight (kDa)

9.05

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 4 - 42 1.1e-06 F-box domain
LRR_At1g61320_AtMIF1 PF23622 84 - 380 2.1e-12 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 94 - 246 1.4e-14 FBD-associated F-box protein At5g56370, LRR repeats
FBD PF08387 333 - 378 2.2e-16 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05080 AT1G19410 AT1G22000 AT1G26815 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G32375 AT1G32375 AT1G50980 AT1G51055 AT1G55030 AT1G55030 AT1G55660 AT1G55660 AT2G04230 AT2G26860 AT2G26860 AT3G10750 AT3G26920 AT3G26920 AT3G26920 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26930 AT3G26930 AT3G49020 AT3G49030 AT3G49030 AT3G49040 AT3G49045 AT3G49480 AT3G49480 AT3G50710 AT3G51530 AT3G52680 AT3G52680 AT3G52690 AT3G53550 AT3G54910 AT3G54910 AT3G54910 AT3G55670 AT4G00160 AT4G00315 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G10400 AT4G10400 AT4G10400 AT4G10410 AT4G10420 AT4G13965 AT4G15060 AT4G15060 AT4G15075 AT4G26340 AT4G26350 AT5G11370 AT5G38565 AT5G38570 AT5G38580 AT5G38590 AT5G38590 AT5G38590 AT5G38590 AT5G40750 AT5G44850 AT5G50270 AT5G50270 AT5G52460 AT5G52460 AT5G56325 AT5G56370 AT5G56370 AT5G56370 AT5G56380 AT5G56380 AT5G56390 AT5G56400 AT5G56410 AT5G56420 AT5G56420 AT5G56420 AT5G56430 AT5G56440 AT5G56452 AT5G56452 AT5G56555 AT5G56560 AT5G56560 AT5G56570 AT5G56690 AT5G56700 AT5G56700 AT5G56800 AT5G56810 AT5G56810 AT5G56820 AT5G60610 AT5G60610 AT5G62970
malus_domestica MD10G1316000.v1.1
prunus_persica Prupe.4G024600_v2.0.a1
pyrus_communis pycom10g26820
rosa_chinensis RchiOBHm_Chr1g0383101 RchiOBHm_Chr5g0003811 RchiOBHm_Chr5g0069761 RchiOBHm_Chr5g0069771 RchiOBHm_Chr5g0069781 RchiOBHm_Chr5g0069791 RchiOBHm_Chr5g0069821 RchiOBHm_Chr5g0069831
rosa_laevigata RLG00000026130 RLG00000026375 RLG00000031193 RLG00000036088 RLG00000036089 RLG00000036090 RLG00000036095 RLG00000036096 RLG00000036097 RLG00000036099 RLG00000036100
rosa_multiflora Rmu_co8192842.1_g000001 Rmu_co8209784.1_g000001 Rmu_sc0000726.1_g000001 Rmu_sc0000726.1_g000003 Rmu_sc0000726.1_g000007 Rmu_sc0000726.1_g000008 Rmu_sc0000738.1_g000009 Rmu_sc0000738.1_g000010 Rmu_sc0000738.1_g000011 Rmu_sc0000738.1_g000015 Rmu_sc0000738.1_g000018 Rmu_sc0000738.1_g000019 Rmu_sc0004212.1_g000009 Rmu_sc0017283.1_g000003 Rmu_sc0024481.1_g000001
rosa_roxburghii Rroxscaffold_1G00011240 Rroxscaffold_1G00011270 Rroxscaffold_1G00011280 Rroxscaffold_1G00011300 Rroxscaffold_1G00011310 Rroxscaffold_1G00011320 Rroxscaffold_4G00277590
rosa_rugosa Rorug01G0413100 Rorug01G0438500 Rorug01G0438600 Rorug04G0406800 Rorug05G0399300 Rorug05G0399400 Rorug05G0399500 Rorug05G0399600 Rorug05G0399700 Rorug05G0399900.1 Rorug05G0400000 Rorug05G0400100
rosa_samantha Rh1AG436100 Rh1AG464200 Rh1BG421500 Rh1CG434900 Rh1DG454100 Rh5AG034000 Rh5AG034100 Rh5AG456400 Rh5AG456500 Rh5AG456600 Rh5AG456800 Rh5AG457000 Rh5BG032600 Rh5BG475500 Rh5BG475600 Rh5BG475800 Rh5BG475900 Rh5CG036600 Rh5CG036700 Rh5CG499100 Rh5CG499200 Rh5CG499400 Rh5CG499600 Rh5DG032800 Rh5DG487000 Rh5DG487100 Rh5DG487200 Rh5DG487400 Rh5DG487500
rosa_wichuraiana Rw5G003130 Rw5G042610 Rw5G042630 Rw5G042650 Rw5G042660 Rw5G042670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 389
AccB7I CCANNNNNTGG 1 cut(s) 682
AccI GTMKAC 1 cut(s) 1033
AciI CCGC 5 cut(s) 414, 511, 805, 1088, 1114
AclI AACGTT 2 cut(s) 148, 610
AclWI GGATC 3 cut(s) 508, 693, 793
AcsI RAATTY 2 cut(s) 164, 1040
AcuI CTGAAG 2 cut(s) 341, 491
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 2 cut(s) 682, 862
AflII CTTAAG 2 cut(s) 131, 1117
AflIII ACRYGT 1 cut(s) 1145
AgsI TTSAA 7 cut(s) 169, 403, 460, 493, 539, 853, 1102
AjnI CCWGG 1 cut(s) 989
AluBI AGCT 6 cut(s) 215, 319, 372, 583, 733, 1199
AluI AGCT 6 cut(s) 215, 319, 372, 583, 733, 1199
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 3 cut(s) 782, 955, 1177
AlwI GGATC 3 cut(s) 508, 693, 793
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 1163
ApoI RAATTY 2 cut(s) 164, 1040
ArsI GACNNNNNNTTYG 2 cut(s) 430, 462
AsuHPI GGTGA 1 cut(s) 848
AsuII TTCGAA 1 cut(s) 893
AvaI CYCGRG 1 cut(s) 1163
AxyI CCTNAGG 1 cut(s) 267
BaeI ACNNNNGTAYC 2 cut(s) 35, 68
BanII GRGCYC 1 cut(s) 217
BauI CACGAG 1 cut(s) 392
BbsI GAAGAC 2 cut(s) 440, 1072
Bbv12I GWGCWC 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 734
BccI CCATC 3 cut(s) 93, 404, 607
BcgI CGANNNNNNTGC 2 cut(s) 373, 407
BciT130I CCWGG 1 cut(s) 991
BclI TGATCA 1 cut(s) 964
BcoDI GTCTC 3 cut(s) 782, 955, 1177
BfaI CTAG 2 cut(s) 545, 633
BfrI CTTAAG 2 cut(s) 131, 1117
BfuAI ACCTGC 1 cut(s) 389
BglII AGATCT 1 cut(s) 541
BisI GCNGC 3 cut(s) 512, 806, 1114
BlsI GCNGC 3 cut(s) 513, 807, 1115
BmcAI AGTACT 1 cut(s) 33
Bme1390I CCNGG 1 cut(s) 991
BmeT110I CYCGRG 1 cut(s) 1163
BmiI GGNNCC 1 cut(s) 911
BmrFI CCNGG 1 cut(s) 991
BpiI GAAGAC 2 cut(s) 440, 1072
Bpu10I CCTNAGC 2 cut(s) 729, 734
Bpu14I TTCGAA 1 cut(s) 893
BpuEI CTTGAG 1 cut(s) 881
BsaAI YACGTR 1 cut(s) 314
BsaBI GATNNNNATC 1 cut(s) 195
Bsc4I CCNNNNNNNGG 2 cut(s) 682, 862
Bse1I ACTGG 1 cut(s) 39
Bse21I CCTNAGG 1 cut(s) 267
Bse8I GATNNNNATC 1 cut(s) 195
BseBI CCWGG 1 cut(s) 991
BseGI GGATG 3 cut(s) 120, 433, 967
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 682, 862
BseMII CTCAG 3 cut(s) 258, 725, 813
BseNI ACTGG 1 cut(s) 39
BseRI GAGGAG 1 cut(s) 890
BsiHKAI GWGCWC 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 1163
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 2 cut(s) 682, 862
BsmAI GTCTC 3 cut(s) 782, 955, 1177
BsmBI CGTCTC 1 cut(s) 955
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 1 cut(s) 1022
BsoBI CYCGRG 1 cut(s) 1163
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 6 cut(s) 396, 500, 541, 685, 785, 964
BspACI CCGC 5 cut(s) 414, 511, 805, 1088, 1114
BspCNI CTCAG 3 cut(s) 259, 726, 814
BspHI TCATGA 2 cut(s) 565, 967
BspLI GGNNCC 1 cut(s) 911
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 3 cut(s) 508, 693, 793
BspT104I TTCGAA 1 cut(s) 893
BspTI CTTAAG 2 cut(s) 131, 1117
BsrI ACTGG 1 cut(s) 39
BssMI GATC 6 cut(s) 396, 500, 541, 685, 785, 964
BssSI CACGAG 1 cut(s) 392
Bst2BI CACGAG 1 cut(s) 392
Bst2UI CCWGG 1 cut(s) 991
Bst4CI ACNGT 5 cut(s) 48, 180, 589, 669, 836
Bst6I CTCTTC 1 cut(s) 510
BstAFI CTTAAG 2 cut(s) 131, 1117
BstBAI YACGTR 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 2 cut(s) 810, 1111
BstDEI CTNAG 5 cut(s) 267, 729, 734, 822, 1060
BstEII GGTNACC 1 cut(s) 18
BstF5I GGATG 3 cut(s) 120, 433, 967
BstKTI GATC 6 cut(s) 399, 503, 544, 688, 788, 967
BstMAI GTCTC 3 cut(s) 782, 955, 1177
BstMBI GATC 6 cut(s) 396, 500, 541, 685, 785, 964
BstNI CCWGG 1 cut(s) 991
BstNSI RCATGY 2 cut(s) 1024, 1212
BstPI GGTNACC 1 cut(s) 18
BstSCI CCNGG 1 cut(s) 989
BstV2I GAAGAC 2 cut(s) 440, 1072
BstX2I RGATCY 2 cut(s) 500, 541
BstYI RGATCY 2 cut(s) 500, 541
Bsu36I CCTNAGG 1 cut(s) 267
BtsCI GGATG 3 cut(s) 120, 433, 967
BtsIMutI CAGTG 2 cut(s) 19, 185
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 2 cut(s) 810, 1111
CaiI CAGNNNCTG 1 cut(s) 161
CciI TCATGA 2 cut(s) 565, 967
Csp6I GTAC 1 cut(s) 32
CviAII CATG 4 cut(s) 566, 968, 1021, 1209
CviJI RGCY 8 cut(s) 215, 319, 372, 514, 583, 733, 808, 1199
CviKI_1 RGCY 8 cut(s) 215, 319, 372, 514, 583, 733, 808, 1199
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 5 cut(s) 267, 729, 734, 822, 1060
DpnI GATC 6 cut(s) 398, 502, 543, 687, 787, 966
DpnII GATC 6 cut(s) 396, 500, 541, 685, 785, 964
DraI TTTAAA 2 cut(s) 262, 1054
Eam1104I CTCTTC 1 cut(s) 510
EarI CTCTTC 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 217
Eco32I GATATC 2 cut(s) 274, 769
Eco53kI GAGCTC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 341, 491
Eco81I CCTNAGG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 1163
Eco91I GGTNACC 1 cut(s) 18
EcoICRI GAGCTC 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 989
EcoRV GATATC 2 cut(s) 274, 769
EcoT22I ATGCAT 1 cut(s) 1022
EcoT38I GRGCYC 1 cut(s) 217
Esp3I CGTCTC 1 cut(s) 955
FaeI CATG 4 cut(s) 569, 971, 1024, 1212
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FaqI GGGAC 1 cut(s) 581
FatI CATG 4 cut(s) 565, 967, 1020, 1208
FauI CCCGC 1 cut(s) 407
FbaI TGATCA 1 cut(s) 964
FblI GTMKAC 1 cut(s) 1033
Fnu4HI GCNGC 3 cut(s) 512, 806, 1114
FokI GGATG 3 cut(s) 127, 440, 974
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 3 cut(s) 512, 806, 1114
FspBI CTAG 2 cut(s) 545, 633
GluI GCNGC 3 cut(s) 512, 806, 1114
Hin1II CATG 4 cut(s) 569, 971, 1024, 1212
HincII GTYRAC 2 cut(s) 78, 123
HindII GTYRAC 2 cut(s) 78, 123
HindIII AAGCTT 2 cut(s) 581, 1197
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 6 cut(s) 78, 123, 146, 1034, 1093, 1155
Hpy188I TCNGA 8 cut(s) 60, 157, 163, 278, 785, 823, 955, 1141
Hpy8I GTNNAC 6 cut(s) 78, 123, 146, 1034, 1093, 1155
HpyAV CCTTC 3 cut(s) 639, 669, 833
HpyCH4III ACNGT 5 cut(s) 48, 180, 589, 669, 836
HpyCH4IV ACGT 4 cut(s) 148, 313, 610, 1147
HpyCH4V TGCA 5 cut(s) 384, 607, 812, 1020, 1127
HpyF3I CTNAG 5 cut(s) 267, 729, 734, 822, 1060
HpySE526I ACGT 4 cut(s) 148, 313, 610, 1147
Hsp92II CATG 4 cut(s) 569, 971, 1024, 1212
Ksp22I TGATCA 1 cut(s) 964
Kzo9I GATC 6 cut(s) 396, 500, 541, 685, 785, 964
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 9 cut(s) 20, 36, 279, 394, 543, 892, 976, 1003, 1026
MaeI CTAG 2 cut(s) 545, 633
MaeII ACGT 4 cut(s) 148, 313, 610, 1147
MaeIII GTNAC 4 cut(s) 18, 174, 701, 830
MalI GATC 6 cut(s) 398, 502, 543, 687, 787, 966
MboI GATC 6 cut(s) 396, 500, 541, 685, 785, 964
MboII GAAGA 8 cut(s) 334, 440, 497, 551, 841, 882, 1077, 1182
MflI RGATCY 2 cut(s) 500, 541
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 3 cut(s) 164, 523, 1040
MlyI GAGTC 3 cut(s) 167, 784, 1039
MmeI TCCRAC 2 cut(s) 83, 700
Mph1103I ATGCAT 1 cut(s) 1022
MseI TTAA 6 cut(s) 39, 132, 245, 261, 1053, 1118
MslI CAYNNNNRTG 1 cut(s) 1207
MspA1I CMGCKG 1 cut(s) 511
MspCI CTTAAG 2 cut(s) 131, 1117
MspR9I CCNGG 1 cut(s) 991
Mva1269I GAATGC 1 cut(s) 1022
MvaI CCWGG 1 cut(s) 991
NdeII GATC 6 cut(s) 396, 500, 541, 685, 785, 964
NlaIII CATG 4 cut(s) 569, 971, 1024, 1212
NlaIV GGNNCC 1 cut(s) 911
NmuCI GTSAC 1 cut(s) 701
NsiI ATGCAT 1 cut(s) 1022
NspI RCATGY 2 cut(s) 1024, 1212
NspV TTCGAA 1 cut(s) 893
PaeR7I CTCGAG 1 cut(s) 1163
PagI TCATGA 2 cut(s) 565, 967
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 310
PctI GAATGC 1 cut(s) 1022
PfeI GAWTC 7 cut(s) 9, 196, 251, 553, 895, 973, 994
PflMI CCANNNNNTGG 1 cut(s) 682
PfoI TCCNGGA 1 cut(s) 989
PkrI GCNGC 3 cut(s) 513, 807, 1115
PleI GAGTC 3 cut(s) 166, 783, 1038
PpsI GAGTC 3 cut(s) 166, 783, 1038
Ppu21I YACGTR 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 217
Psp1406I AACGTT 2 cut(s) 148, 610
Psp6I CCWGG 1 cut(s) 989
PspEI GGTNACC 1 cut(s) 18
PspGI CCWGG 1 cut(s) 989
PspN4I GGNNCC 1 cut(s) 911
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 2 cut(s) 500, 541
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 1207
SacI GAGCTC 1 cut(s) 217
SaqAI TTAA 6 cut(s) 39, 132, 245, 261, 1053, 1118
SatI GCNGC 3 cut(s) 512, 806, 1114
Sau3AI GATC 6 cut(s) 396, 500, 541, 685, 785, 964
ScaI AGTACT 1 cut(s) 33
SchI GAGTC 3 cut(s) 167, 784, 1039
ScrFI CCNGG 1 cut(s) 991
SduI GDGCHC 1 cut(s) 217
Sfr274I CTCGAG 1 cut(s) 1163
SfuI TTCGAA 1 cut(s) 893
SlaI CTCGAG 1 cut(s) 1163
SmiMI CAYNNNNRTG 1 cut(s) 1207
SmlI CTYRAG 4 cut(s) 131, 860, 1117, 1163
SmoI CTYRAG 4 cut(s) 131, 860, 1117, 1163
Sse9I AATT 3 cut(s) 164, 523, 1040
SsiI CCGC 5 cut(s) 414, 511, 805, 1088, 1114
SspMI CTAG 2 cut(s) 545, 633
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 1 cut(s) 989
TaaI ACNGT 5 cut(s) 48, 180, 589, 669, 836
TaiI ACGT 4 cut(s) 151, 316, 613, 1150
TaqI TCGA 6 cut(s) 92, 408, 436, 893, 1028, 1164
TasI AATT 3 cut(s) 164, 523, 1040
TatI WGTACW 1 cut(s) 31
TauI GCSGC 3 cut(s) 514, 808, 1116
TfiI GAWTC 7 cut(s) 9, 196, 251, 553, 895, 973, 994
Tru1I TTAA 6 cut(s) 39, 132, 245, 261, 1053, 1118
Tru9I TTAA 6 cut(s) 39, 132, 245, 261, 1053, 1118
TscAI CASTG 2 cut(s) 19, 185
TseFI GTSAC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 701
TspDTI ATGAA 2 cut(s) 42, 1191
TspGWI ACGGA 3 cut(s) 224, 974, 1190
TspRI CASTG 2 cut(s) 19, 185
Van91I CCANNNNNTGG 1 cut(s) 682
Vha464I CTTAAG 2 cut(s) 131, 1117
XapI RAATTY 2 cut(s) 164, 1040
XceI RCATGY 2 cut(s) 1024, 1212
XhoI CTCGAG 1 cut(s) 1163
XmiI GTMKAC 1 cut(s) 1033
XspI CTAG 2 cut(s) 545, 633
ZrmI AGTACT 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.