Rh5DG487000

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000140
Physical Location & Seq
Forward (+)
76626322 .. 76629798
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG487000.1

Sequence Viewer

Length: 1380 bp
ATGGATGCAAGCTCTCAAACAGGACAACCCAAAAAGCGGAAGTTATCTGGGGAAAAAGAGGAAGGAGGCAGCATGAGCTTAAGCAAGTTACCTGATGTGCTTATTGAACAAATTATCTCCTTCCTTCCAACAAAAGATGCTATCCGAACAAGCATTCTGTCTAAAAGATGGGAATACCTATGGACATCACTTCCCGAACTTAAGTTCATTCAAGGGATGAATGAAACGAGATGTTCCTTTGTGACTGCTGTGGACAGGGCACTAATTCTTCGTGGCCCTGCTGATCTAAAACAGTTTCTTCTTTTGTTTAGAGGGTTAGAGCAGCTAGATGATGCATCTCGTGTTAATCGGTGGATCACTGCCATGCTAAGACGTAATGTTGAAGAGCTTATACTTCGCCTGTCTAACTATAAGGAGCTTTCACTGCCTCGTTCTTTATTTATTTCTGCAACACTCACGTACTTGGACATAATTGTGCCCTGTATTTTCAAAGTTCCTTCTACTGTTTGTCTATCAAGTTTAAGGACCTTGAGACTTCAATGCGTGGTGTTTTCTGATGACAGCTCAACCCAGCAGCTGTTTTCAGGTTGTCCAGTCCTTGAGGACTTGATACTAGGAGAATGCAGCTGGGGCAATTGCAAGTTTGTGAGTATTTGTGCCCCAAAACTTTTGAGTTTGGAAATTGATGAAGACATTGAATTATGTCCCAAAGGTTCAGATGGTTGCCAGATTATGATATTTGGTGTTAATCTCACAAACTTTTATTACTCGGGTGAATTCCAAAATCATTATTGCTTCAATAACTCATCAGTAAATGAGGCTCATATTTTTCATTGCTCTAATGAGAAGTCGAGGCATATGAGTTATGGGTTTAATAAGCTTCTTGGAGGACTATCTAGTGTGAAGACCCTAGCTACCAATTCTGATTCCCTTGAGGTGATTCAGACTCATGCAACAGAACTGCTTACCCAGATGCCCCTGTTGAATTACCTAACCACTTTGAAATTGGAAGCAGAGGAAGTAGTTCCCTGTAGCAAAGCACTATTGAGGATGCTTGGAAACTGTCCTTGCCTTCAAACTCTGATATTCTTTCAGGAGATTAACCTGTCCTCAGATGATGCTAATGACGATGGGGTGTTGGAGCCACTGCCTCCATGTTTCCTGACTAGTCTTAAACAGATTGAAGTTCATGCATTCTATGGAGACGAGAATAATCTTTGTGCAATAAAGATTTTGCTAAAGAATGCCATGGTCTTGGAGAAGATGATCTTAACTTGCATGCACAATTCTGTTGGAGGTTCAGAGAAGAAAAAGGATGTTAACAAACAGCTATCAGATCTCCCAAAAGGGTCAGAAAGCTGTGAAGTTGTTCTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

459

Amino Acids

51.36

Weight (kDa)

5.46

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 27 - 66 1.6e-09 F-box domain
LRR_At5g56370 PF24758 115 - 227 1.9e-12 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 173 - 198 8e-06 Leucine Rich Repeat
FBD PF08387 383 - 424 3.5e-06 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05080 AT1G19410 AT1G22000 AT1G26815 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G32375 AT1G32375 AT1G50980 AT1G51055 AT1G55030 AT1G55030 AT1G55660 AT1G55660 AT2G04230 AT2G26860 AT2G26860 AT3G10750 AT3G26920 AT3G26920 AT3G26920 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26930 AT3G26930 AT3G49020 AT3G49030 AT3G49030 AT3G49040 AT3G49045 AT3G49480 AT3G49480 AT3G50710 AT3G51530 AT3G52680 AT3G52680 AT3G52690 AT3G53550 AT3G54910 AT3G54910 AT3G54910 AT3G55670 AT4G00160 AT4G00315 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G10400 AT4G10400 AT4G10400 AT4G10410 AT4G10420 AT4G13965 AT4G15060 AT4G15060 AT4G15075 AT4G26340 AT4G26350 AT5G11370 AT5G38565 AT5G38570 AT5G38580 AT5G38590 AT5G38590 AT5G38590 AT5G38590 AT5G40750 AT5G44850 AT5G50270 AT5G50270 AT5G52460 AT5G52460 AT5G56325 AT5G56370 AT5G56370 AT5G56370 AT5G56380 AT5G56380 AT5G56390 AT5G56400 AT5G56410 AT5G56420 AT5G56420 AT5G56420 AT5G56430 AT5G56440 AT5G56452 AT5G56452 AT5G56555 AT5G56560 AT5G56560 AT5G56570 AT5G56690 AT5G56700 AT5G56700 AT5G56800 AT5G56810 AT5G56810 AT5G56820 AT5G60610 AT5G60610 AT5G62970
malus_domestica MD10G1316000.v1.1
prunus_persica Prupe.4G024600_v2.0.a1
pyrus_communis pycom10g26820
rosa_chinensis RchiOBHm_Chr1g0383101 RchiOBHm_Chr5g0003811 RchiOBHm_Chr5g0069761 RchiOBHm_Chr5g0069771 RchiOBHm_Chr5g0069781 RchiOBHm_Chr5g0069791 RchiOBHm_Chr5g0069821 RchiOBHm_Chr5g0069831
rosa_laevigata RLG00000026130 RLG00000026375 RLG00000031193 RLG00000036088 RLG00000036089 RLG00000036090 RLG00000036095 RLG00000036096 RLG00000036097 RLG00000036099 RLG00000036100
rosa_multiflora Rmu_co8192842.1_g000001 Rmu_co8209784.1_g000001 Rmu_sc0000726.1_g000001 Rmu_sc0000726.1_g000003 Rmu_sc0000726.1_g000007 Rmu_sc0000726.1_g000008 Rmu_sc0000738.1_g000009 Rmu_sc0000738.1_g000010 Rmu_sc0000738.1_g000011 Rmu_sc0000738.1_g000015 Rmu_sc0000738.1_g000018 Rmu_sc0000738.1_g000019 Rmu_sc0004212.1_g000009 Rmu_sc0017283.1_g000003 Rmu_sc0024481.1_g000001
rosa_roxburghii Rroxscaffold_1G00011240 Rroxscaffold_1G00011270 Rroxscaffold_1G00011280 Rroxscaffold_1G00011300 Rroxscaffold_1G00011310 Rroxscaffold_1G00011320 Rroxscaffold_4G00277590
rosa_rugosa Rorug01G0413100 Rorug01G0438500 Rorug01G0438600 Rorug04G0406800 Rorug05G0399300 Rorug05G0399400 Rorug05G0399500 Rorug05G0399600 Rorug05G0399700 Rorug05G0399900.1 Rorug05G0400000 Rorug05G0400100
rosa_samantha Rh1AG436100 Rh1AG464200 Rh1BG421500 Rh1CG434900 Rh1DG454100 Rh5AG034000 Rh5AG034100 Rh5AG456400 Rh5AG456500 Rh5AG456600 Rh5AG456800 Rh5AG457000 Rh5BG032600 Rh5BG475500 Rh5BG475600 Rh5BG475800 Rh5BG475900 Rh5CG036600 Rh5CG036700 Rh5CG499100 Rh5CG499200 Rh5CG499400 Rh5CG499600 Rh5DG032800 Rh5DG487000 Rh5DG487100 Rh5DG487200 Rh5DG487400 Rh5DG487500
rosa_wichuraiana Rw5G003130 Rw5G042610 Rw5G042630 Rw5G042650 Rw5G042660 Rw5G042670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 37
AclWI GGATC 1 cut(s) 362
AcsI RAATTY 1 cut(s) 776
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 1 cut(s) 36
AflII CTTAAG 2 cut(s) 79, 200
AhlI ACTAGT 1 cut(s) 1166
Alw26I GTCTC 2 cut(s) 526, 1197
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 577
Ama87I CYCGRG 1 cut(s) 769
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 4 cut(s) 69, 322, 574, 624
ApoI RAATTY 1 cut(s) 776
Asp700I GAANNNNTTC 2 cut(s) 1023, 1368
AspS9I GGNCC 2 cut(s) 275, 525
AsuHPI GGTGA 2 cut(s) 785, 949
AvaI CYCGRG 1 cut(s) 769
AvaII GGWCC 1 cut(s) 525
BaeGI GKGCMC 3 cut(s) 262, 480, 661
BarI GAAGNNNNNNTAC 2 cut(s) 375, 407
BauI CACGAG 1 cut(s) 339
BbsI GAAGAC 2 cut(s) 696, 911
BbvI GCAGC 4 cut(s) 81, 334, 586, 636
BccI CCATC 3 cut(s) 162, 713, 1124
BcoDI GTCTC 2 cut(s) 526, 1197
BcuI ACTAGT 1 cut(s) 1166
BfaI CTAG 5 cut(s) 326, 614, 897, 911, 1167
BfmI CTRYAG 1 cut(s) 1030
BfrI CTTAAG 2 cut(s) 79, 200
BglII AGATCT 1 cut(s) 1336
BisI GCNGC 4 cut(s) 70, 323, 575, 625
BlsI GCNGC 4 cut(s) 71, 324, 576, 626
Bme18I GGWCC 1 cut(s) 525
BmeT110I CYCGRG 1 cut(s) 769
BmgT120I GGNCC 2 cut(s) 275, 525
BmiI GGNNCC 1 cut(s) 1143
BmsI GCATC 6 cut(s) 127, 322, 344, 963, 1041, 1108
BpiI GAAGAC 2 cut(s) 696, 911
BpuEI CTTGAG 3 cut(s) 550, 620, 953
BsaAI YACGTR 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 1248
Bsc4I CCNNNNNNNGG 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 593
Bse3DI GCAATG 1 cut(s) 832
BseDI CCNNGG 1 cut(s) 1248
BseGI GGATG 4 cut(s) 10, 222, 1056, 1321
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMI GCAATG 1 cut(s) 832
BseMII CTCAG 1 cut(s) 1125
BseNI ACTGG 1 cut(s) 593
BseSI GKGCMC 3 cut(s) 262, 480, 661
BseXI GCAGC 4 cut(s) 81, 334, 586, 636
BseYI CCCAGC 2 cut(s) 570, 627
BshFI GGCC 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 769
BslFI GGGAC 1 cut(s) 690
BslI CCNNNNNNNGG 1 cut(s) 36
BsmAI GTCTC 2 cut(s) 526, 1197
BsmBI CGTCTC 1 cut(s) 1197
BsmFI GGGAC 1 cut(s) 690
BsmI GAATGC 4 cut(s) 153, 626, 1193, 1249
BsnI GGCC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 769
Bsp1286I GDGCHC 3 cut(s) 262, 480, 661
Bsp143I GATC 4 cut(s) 283, 354, 1266, 1336
Bsp19I CCATGG 1 cut(s) 1248
BspACI CCGC 1 cut(s) 37
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 1 cut(s) 1124
BspLI GGNNCC 1 cut(s) 1143
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 378
BspTI CTTAAG 2 cut(s) 79, 200
BsrDI GCAATG 1 cut(s) 832
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 1 cut(s) 1248
BssMI GATC 4 cut(s) 283, 354, 1266, 1336
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 1248
Bst2BI CACGAG 1 cut(s) 339
Bst4CI ACNGT 3 cut(s) 294, 505, 1064
Bst6I CTCTTC 1 cut(s) 378
BstAFI CTTAAG 2 cut(s) 79, 200
BstBAI YACGTR 1 cut(s) 459
BstC8I GCNNGC 2 cut(s) 10, 1280
BstDEI CTNAG 2 cut(s) 368, 1111
BstDSI CCRYGG 1 cut(s) 1248
BstF5I GGATG 4 cut(s) 10, 222, 1056, 1321
BstKTI GATC 4 cut(s) 286, 357, 1269, 1339
BstMAI GTCTC 2 cut(s) 526, 1197
BstMBI GATC 4 cut(s) 283, 354, 1266, 1336
BstMWI GCNNNNNNNGC 3 cut(s) 75, 424, 630
BstNSI RCATGY 1 cut(s) 1282
BstSFI CTRYAG 1 cut(s) 1030
BstSLI GKGCMC 3 cut(s) 262, 480, 661
BstV1I GCAGC 4 cut(s) 81, 334, 586, 636
BstV2I GAAGAC 2 cut(s) 696, 911
BstX2I RGATCY 1 cut(s) 1336
BstXI CCANNNNNNTGG 1 cut(s) 1255
BstYI RGATCY 1 cut(s) 1336
BsuRI GGCC 1 cut(s) 276
BtgI CCRYGG 1 cut(s) 1248
BtsCI GGATG 4 cut(s) 10, 222, 1056, 1321
BtsI GCAGTG 3 cut(s) 357, 422, 1145
BtsIMutI CAGTG 3 cut(s) 357, 422, 1145
Cac8I GCNNGC 2 cut(s) 10, 1280
CaiI CAGNNNCTG 1 cut(s) 577
Cfr13I GGNCC 2 cut(s) 275, 525
Csp6I GTAC 1 cut(s) 460
CviAII CATG 7 cut(s) 73, 364, 950, 1155, 1190, 1249, 1279
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 2 cut(s) 368, 1111
DpnI GATC 4 cut(s) 285, 356, 1268, 1338
DpnII GATC 4 cut(s) 283, 354, 1266, 1336
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 1248
Eco47I GGWCC 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 769
EcoO109I RGGNCCY 1 cut(s) 525
EcoRI GAATTC 1 cut(s) 776
EcoT14I CCWWGG 1 cut(s) 1248
EcoT22I ATGCAT 2 cut(s) 337, 1195
ErhI CCWWGG 1 cut(s) 1248
Esp3I CGTCTC 1 cut(s) 1197
FaeI CATG 7 cut(s) 76, 367, 953, 1158, 1193, 1252, 1282
FalI AAGNNNNNCTT 3 cut(s) 1253, 1285, 1356
FaqI GGGAC 1 cut(s) 690
FatI CATG 7 cut(s) 72, 363, 949, 1154, 1189, 1248, 1278
FauNDI CATATG 1 cut(s) 858
Fnu4HI GCNGC 4 cut(s) 70, 323, 575, 625
FokI GGATG 4 cut(s) 17, 229, 1063, 1328
Fsp4HI GCNGC 4 cut(s) 70, 323, 575, 625
FspBI CTAG 5 cut(s) 326, 614, 897, 911, 1167
GluI GCNGC 4 cut(s) 70, 323, 575, 625
GsaI CCCAGC 2 cut(s) 574, 631
HaeIII GGCC 1 cut(s) 276
Hin1II CATG 7 cut(s) 76, 367, 953, 1158, 1193, 1252, 1282
HincII GTYRAC 1 cut(s) 1321
HindII GTYRAC 1 cut(s) 1321
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 3 cut(s) 926, 940, 946
HpaI GTTAAC 1 cut(s) 1321
HphI GGTGA 2 cut(s) 785, 949
Hpy166II GTNNAC 2 cut(s) 253, 1321
Hpy188III TCNNGA 3 cut(s) 194, 1094, 1162
Hpy8I GTNNAC 2 cut(s) 253, 1321
HpyAV CCTTC 5 cut(s) 56, 130, 134, 507, 1082
HpyCH4III ACNGT 3 cut(s) 294, 505, 1064
HpyCH4IV ACGT 2 cut(s) 373, 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 424, 630
HpyF3I CTNAG 2 cut(s) 368, 1111
HpySE526I ACGT 2 cut(s) 373, 458
Hsp92II CATG 7 cut(s) 76, 367, 953, 1158, 1193, 1252, 1282
KspAI GTTAAC 1 cut(s) 1321
Kzo9I GATC 4 cut(s) 283, 354, 1266, 1336
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 2 cut(s) 415, 1141
Lsp1109I GCAGC 4 cut(s) 81, 334, 586, 636
LweI GCATC 6 cut(s) 127, 322, 344, 963, 1041, 1108
MaeI CTAG 5 cut(s) 326, 614, 897, 911, 1167
MaeII ACGT 2 cut(s) 373, 458
MaeIII GTNAC 2 cut(s) 87, 241
MalI GATC 4 cut(s) 285, 356, 1268, 1338
MboI GATC 4 cut(s) 283, 354, 1266, 1336
MboII GAAGA 7 cut(s) 260, 290, 395, 701, 916, 1273, 1318
MfeI CAATTG 1 cut(s) 634
MflI RGATCY 1 cut(s) 1336
MhlI GDGCHC 3 cut(s) 262, 480, 661
MlyI GAGTC 1 cut(s) 940
MmeI TCCRAC 3 cut(s) 152, 1119, 1273
Mph1103I ATGCAT 2 cut(s) 337, 1195
MroXI GAANNNNTTC 2 cut(s) 1023, 1368
MslI CAYNNNNRTG 2 cut(s) 362, 473
MspA1I CMGCKG 2 cut(s) 577, 627
MspCI CTTAAG 2 cut(s) 79, 200
MunI CAATTG 1 cut(s) 634
Mva1269I GAATGC 4 cut(s) 153, 626, 1193, 1249
MwoI GCNNNNNNNGC 3 cut(s) 75, 424, 630
NcoI CCATGG 1 cut(s) 1248
NdeI CATATG 1 cut(s) 858
NdeII GATC 4 cut(s) 283, 354, 1266, 1336
NlaIII CATG 7 cut(s) 76, 367, 953, 1158, 1193, 1252, 1282
NlaIV GGNNCC 1 cut(s) 1143
NmuCI GTSAC 1 cut(s) 241
NsiI ATGCAT 2 cut(s) 337, 1195
NspI RCATGY 1 cut(s) 1282
PaeI GCATGC 1 cut(s) 1282
PciSI GCTCTTC 1 cut(s) 378
PctI GAATGC 4 cut(s) 153, 626, 1193, 1249
PdmI GAANNNNTTC 2 cut(s) 1023, 1368
PfeI GAWTC 2 cut(s) 926, 940
PkrI GCNGC 4 cut(s) 71, 324, 576, 626
PleI GAGTC 1 cut(s) 940
PpsI GAGTC 1 cut(s) 940
Ppu21I YACGTR 1 cut(s) 459
PpuMI RGGWCCY 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 525
PspFI CCCAGC 2 cut(s) 570, 627
PspN4I GGNNCC 1 cut(s) 1143
PspPI GGNCC 2 cut(s) 275, 525
PspPPI RGGWCCY 1 cut(s) 525
PstNI CAGNNNCTG 1 cut(s) 577
PsuI RGATCY 1 cut(s) 1336
PvuII CAGCTG 2 cut(s) 577, 627
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
RseI CAYNNNNRTG 2 cut(s) 362, 473
SapI GCTCTTC 1 cut(s) 378
SatI GCNGC 4 cut(s) 70, 323, 575, 625
Sau3AI GATC 4 cut(s) 283, 354, 1266, 1336
Sau96I GGNCC 2 cut(s) 275, 525
SchI GAGTC 1 cut(s) 940
SduI GDGCHC 3 cut(s) 262, 480, 661
SfaNI GCATC 6 cut(s) 127, 322, 344, 963, 1041, 1108
SfcI CTRYAG 1 cut(s) 1030
SinI GGWCC 1 cut(s) 525
SmiMI CAYNNNNRTG 2 cut(s) 362, 473
SmlI CTYRAG 5 cut(s) 79, 200, 529, 599, 932
SmoI CTYRAG 5 cut(s) 79, 200, 529, 599, 932
SpeI ACTAGT 1 cut(s) 1166
SphI GCATGC 1 cut(s) 1282
SsiI CCGC 1 cut(s) 37
SspMI CTAG 5 cut(s) 326, 614, 897, 911, 1167
StyI CCWWGG 1 cut(s) 1248
TaaI ACNGT 3 cut(s) 294, 505, 1064
TaiI ACGT 2 cut(s) 376, 461
TaqI TCGA 1 cut(s) 851
TfiI GAWTC 2 cut(s) 926, 940
TscAI CASTG 3 cut(s) 364, 429, 1152
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 4 cut(s) 69, 322, 574, 624
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 6 cut(s) 196, 233, 237, 702, 821, 1178
TspRI CASTG 3 cut(s) 364, 429, 1152
Vha464I CTTAAG 2 cut(s) 79, 200
VpaK11BI GGWCC 1 cut(s) 525
XapI RAATTY 1 cut(s) 776
XceI RCATGY 1 cut(s) 1282
XcmI CCANNNNNNNNNTGG 1 cut(s) 1003
XmnI GAANNNNTTC 2 cut(s) 1023, 1368
XspI CTAG 5 cut(s) 326, 614, 897, 911, 1167
Zsp2I ATGCAT 2 cut(s) 337, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.