AT5G50270

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000140
Physical Location & Seq
Forward (+)
20457487 .. 20458996
1510 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50270.1

Sequence Viewer

Length: 1227 bp
ATGGATAGGATAAGTGGGCTGCCTGATGAGCTACTCTTGAGAGTATTGTCGTTACTACCTAATGTGAAAGATGTTGTTGTCACAATGGTTTTGTCTAAACGATGGCAGTTTCTTTGGATGATGGTGCCAAAACTTGTTTATGATGATAGCTATCAGAATCTCGAGTATGGAAAGTTTTCACGGTTTGTTGACAGATCTTTGTTTATGCGAAAGGCTCCAGGTATAGAAACTTTGCATTTCAAACTAGGTCAAAACTGTGGTAATGGAGATATTCAATGGTGGATTAGAGCTGCTAGTAAATTTTGTTTTCGTGAGCTGATCATCGAAATCAACTGCTCTACTAGTGCATCTCCATCCATACTTCCAAGGAGTTTGTACACAGAGTGTAGAATGCTTGTGACGCTGAAACTAAAAAAAGCGGTTCTTGTAGATGTTTCTTCACCGACGTGTTTTCCATCCCTCAAAAATTTGAGCCTTGTATCTGTGAAGTACCCTGGTAATGAATTTGTCAAGAGTCTTTTATCAAGTTGTCATGTTCTTGAAGACTTGGTTGTGGAACAATGTATAAATGATAATGTGACTATCTTCTCCGTTAAAGTTCCTTCTCTAAAGAGTTTAGTCTTGCGTACATCAAAAGAGAGAGCTCCAGATGGGGAAAGTGGGTTTGTGGTAGAGGCCCCTTCTTTGGAATACTTGGACATTGATCAAACGGGTGGGTTTTGTGTCATTGAGAATGGAATGCCTAATCTTGCTGAGGCATATGTTAGTGTTCTTCACCATCATCCAGTGAAGTTCCTGAGTTCTATTACTTCAGTCAAGCGTCTCTATTTATGTTTATTACCACACAGTATTCTGTCTTGTATGCAGGATATGTATCCTATTAGATGTGTCTTCCACCGTCTTGTACATATCACGCTATGCACTTGTGACGATGAGTGGTTGAATCTACTTGCGTGTCTTCTCAAAGGTTCCCCTAAACTAATATCTCTCAAACTTGAAAAGCACCATGGCCATTTGATTTGTTCACCAAGCCCTTTACGGGATGATCTGAGTTCAGTTCCTGAATGTGTTTTATCGAGTCTTGAAACCGTTGAATGGGTTGACTATGAAGGAACAGAAGCAGAGAGACAACTGGTAGAGTTTATCTTGAGAAACGGAAGCTGTTTAAAAAAGTTCGTTATCTCCCCCGAATCCGTTAATCCCGACAAAAAATATGAGATGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

408

Amino Acids

46.19

Weight (kDa)

6.6

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 94 - 234 2.7e-12 FBD-associated F-box protein At5g56370, LRR repeats
LRR_2 PF07723 153 - 178 7e-06 Leucine Rich Repeat
FBD PF08387 351 - 394 4.4e-20 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05080 AT1G19410 AT1G22000 AT1G26815 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G32375 AT1G32375 AT1G50980 AT1G51055 AT1G55030 AT1G55030 AT1G55660 AT1G55660 AT2G04230 AT2G26860 AT2G26860 AT3G10750 AT3G26920 AT3G26920 AT3G26920 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26930 AT3G26930 AT3G49020 AT3G49030 AT3G49030 AT3G49040 AT3G49045 AT3G49480 AT3G49480 AT3G50710 AT3G51530 AT3G52680 AT3G52680 AT3G52690 AT3G53550 AT3G54910 AT3G54910 AT3G54910 AT3G55670 AT4G00160 AT4G00315 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G10400 AT4G10400 AT4G10400 AT4G10410 AT4G10420 AT4G13965 AT4G15060 AT4G15060 AT4G15075 AT4G26340 AT4G26350 AT5G11370 AT5G38565 AT5G38570 AT5G38580 AT5G38590 AT5G38590 AT5G38590 AT5G38590 AT5G40750 AT5G44850 AT5G50270 AT5G50270 AT5G52460 AT5G52460 AT5G56325 AT5G56370 AT5G56370 AT5G56370 AT5G56380 AT5G56380 AT5G56390 AT5G56400 AT5G56410 AT5G56420 AT5G56420 AT5G56420 AT5G56430 AT5G56440 AT5G56452 AT5G56452 AT5G56555 AT5G56560 AT5G56560 AT5G56570 AT5G56690 AT5G56700 AT5G56700 AT5G56800 AT5G56810 AT5G56810 AT5G56820 AT5G60610 AT5G60610 AT5G62970
malus_domestica MD10G1316000.v1.1
prunus_persica Prupe.4G024600_v2.0.a1
pyrus_communis pycom10g26820
rosa_chinensis RchiOBHm_Chr1g0383101 RchiOBHm_Chr5g0003811 RchiOBHm_Chr5g0069761 RchiOBHm_Chr5g0069771 RchiOBHm_Chr5g0069781 RchiOBHm_Chr5g0069791 RchiOBHm_Chr5g0069821 RchiOBHm_Chr5g0069831
rosa_laevigata RLG00000026130 RLG00000026375 RLG00000031193 RLG00000036088 RLG00000036089 RLG00000036090 RLG00000036095 RLG00000036096 RLG00000036097 RLG00000036099 RLG00000036100
rosa_multiflora Rmu_co8192842.1_g000001 Rmu_co8209784.1_g000001 Rmu_sc0000726.1_g000001 Rmu_sc0000726.1_g000003 Rmu_sc0000726.1_g000007 Rmu_sc0000726.1_g000008 Rmu_sc0000738.1_g000009 Rmu_sc0000738.1_g000010 Rmu_sc0000738.1_g000011 Rmu_sc0000738.1_g000015 Rmu_sc0000738.1_g000018 Rmu_sc0000738.1_g000019 Rmu_sc0004212.1_g000009 Rmu_sc0017283.1_g000003 Rmu_sc0024481.1_g000001
rosa_roxburghii Rroxscaffold_1G00011240 Rroxscaffold_1G00011270 Rroxscaffold_1G00011280 Rroxscaffold_1G00011300 Rroxscaffold_1G00011310 Rroxscaffold_1G00011320 Rroxscaffold_4G00277590
rosa_rugosa Rorug01G0413100 Rorug01G0438500 Rorug01G0438600 Rorug04G0406800 Rorug05G0399300 Rorug05G0399400 Rorug05G0399500 Rorug05G0399600 Rorug05G0399700 Rorug05G0399900.1 Rorug05G0400000 Rorug05G0400100
rosa_samantha Rh1AG436100 Rh1AG464200 Rh1BG421500 Rh1CG434900 Rh1DG454100 Rh5AG034000 Rh5AG034100 Rh5AG456400 Rh5AG456500 Rh5AG456600 Rh5AG456800 Rh5AG457000 Rh5BG032600 Rh5BG475500 Rh5BG475600 Rh5BG475800 Rh5BG475900 Rh5CG036600 Rh5CG036700 Rh5CG499100 Rh5CG499200 Rh5CG499400 Rh5CG499600 Rh5DG032800 Rh5DG487000 Rh5DG487100 Rh5DG487200 Rh5DG487400 Rh5DG487500
rosa_wichuraiana Rw5G003130 Rw5G042610 Rw5G042630 Rw5G042650 Rw5G042660 Rw5G042670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 124
AciI CCGC 1 cut(s) 419
AcoI YGGCCR 1 cut(s) 1009
AcsI RAATTY 3 cut(s) 299, 466, 503
AcuI CTGAAG 1 cut(s) 795
AdeI CACNNNGTG 1 cut(s) 384
AfaI GTAC 4 cut(s) 377, 491, 628, 906
AfiI CCNNNNNNNGG 3 cut(s) 685, 1039, 1095
AflIII ACRYGT 1 cut(s) 446
AgsI TTSAA 7 cut(s) 241, 275, 542, 943, 998, 1085, 1094
AhlI ACTAGT 1 cut(s) 341
AjiI CACGTC 1 cut(s) 447
AjnI CCWGG 2 cut(s) 217, 493
AleI CACNNNNGTG 1 cut(s) 445
AluBI AGCT 6 cut(s) 31, 150, 290, 316, 644, 1161
AluI AGCT 6 cut(s) 31, 150, 290, 316, 644, 1161
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 2 cut(s) 827, 1120
AlwNI CAGNNNCTG 1 cut(s) 1061
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 2 cut(s) 675, 1009
ApeKI GCWGC 2 cut(s) 19, 290
ApoI RAATTY 3 cut(s) 299, 466, 503
Asp700I GAANNNNTTC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 3 cut(s) 432, 767, 1017
AvaI CYCGRG 1 cut(s) 161
BalI TGGCCA 1 cut(s) 1011
BanI GGYRCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 646
BbsI GAAGAC 3 cut(s) 549, 883, 950
Bbv12I GWGCWC 1 cut(s) 646
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 2 cut(s) 6, 277
BccI CCATC 6 cut(s) 96, 115, 361, 463, 644, 786
BciT130I CCWGG 2 cut(s) 219, 495
BciVI GTATCC 1 cut(s) 885
BclI TGATCA 2 cut(s) 318, 703
BcoDI GTCTC 2 cut(s) 827, 1120
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 3 cut(s) 245, 294, 342
BfuI GTATCC 1 cut(s) 885
BglII AGATCT 1 cut(s) 194
BisI GCNGC 2 cut(s) 20, 291
BlsI GCNGC 2 cut(s) 21, 292
Bme1390I CCNGG 2 cut(s) 219, 495
BmeT110I CYCGRG 1 cut(s) 161
BmgBI CACGTC 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 4 cut(s) 126, 216, 678, 970
BmrFI CCNGG 2 cut(s) 219, 495
BmsI GCATC 1 cut(s) 356
BpiI GAAGAC 3 cut(s) 549, 883, 950
BpmI CTGGAG 2 cut(s) 201, 630
Bpu10I CCTNAGC 1 cut(s) 753
BpuEI CTTGAG 2 cut(s) 58, 1168
BsaBI GATNNNNATC 2 cut(s) 150, 873
BsaJI CCNNGG 3 cut(s) 365, 493, 1006
Bsc4I CCNNNNNNNGG 3 cut(s) 685, 1039, 1095
Bse1I ACTGG 2 cut(s) 785, 1137
Bse8I GATNNNNATC 2 cut(s) 150, 873
BseBI CCWGG 2 cut(s) 219, 495
BseDI CCNNGG 3 cut(s) 365, 493, 1006
BseGI GGATG 5 cut(s) 123, 353, 455, 781, 1048
BseJI GATNNNNATC 2 cut(s) 150, 873
BseLI CCNNNNNNNGG 3 cut(s) 685, 1039, 1095
BseMII CTCAG 3 cut(s) 744, 788, 1040
BseNI ACTGG 2 cut(s) 785, 1137
BseXI GCAGC 2 cut(s) 6, 277
BshFI GGCC 2 cut(s) 677, 1011
BshNI GGYRCC 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 1 cut(s) 161
BslI CCNNNNNNNGG 3 cut(s) 685, 1039, 1095
BsmAI GTCTC 2 cut(s) 827, 1120
BsmBI CGTCTC 1 cut(s) 827
BsmI GAATGC 2 cut(s) 396, 744
BsnI GGCC 2 cut(s) 677, 1011
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 646
Bsp1407I TGTACA 2 cut(s) 375, 904
Bsp143I GATC 4 cut(s) 194, 318, 703, 1045
Bsp19I CCATGG 1 cut(s) 1006
BspACI CCGC 1 cut(s) 419
BspANI GGCC 2 cut(s) 677, 1011
BspCNI CTCAG 3 cut(s) 745, 789, 1041
BspLI GGNNCC 4 cut(s) 126, 216, 678, 970
BspT107I GGYRCC 1 cut(s) 124
BsrGI TGTACA 2 cut(s) 375, 904
BsrI ACTGG 2 cut(s) 785, 1137
BssECI CCNNGG 3 cut(s) 365, 493, 1006
BssMI GATC 4 cut(s) 194, 318, 703, 1045
BssT1I CCWWGG 2 cut(s) 365, 1006
Bst2UI CCWGG 2 cut(s) 219, 495
Bst4CI ACNGT 5 cut(s) 183, 257, 848, 899, 1090
BstAUI TGTACA 2 cut(s) 375, 904
BstDEI CTNAG 3 cut(s) 753, 797, 1049
BstDSI CCRYGG 1 cut(s) 1006
BstF5I GGATG 5 cut(s) 123, 353, 455, 781, 1048
BstKTI GATC 4 cut(s) 197, 321, 706, 1048
BstMAI GTCTC 2 cut(s) 827, 1120
BstMBI GATC 4 cut(s) 194, 318, 703, 1045
BstMWI GCNNNNNNNGC 2 cut(s) 28, 400
BstNI CCWGG 2 cut(s) 219, 495
BstSCI CCNGG 2 cut(s) 217, 493
BstV1I GCAGC 2 cut(s) 6, 277
BstV2I GAAGAC 3 cut(s) 549, 883, 950
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 2 cut(s) 677, 1011
BtgI CCRYGG 1 cut(s) 1006
BtrI CACGTC 1 cut(s) 447
BtsCI GGATG 5 cut(s) 123, 353, 455, 781, 1048
BtsIMutI CAGTG 1 cut(s) 792
CaiI CAGNNNCTG 1 cut(s) 1061
Cfr13I GGNCC 1 cut(s) 676
CseI GACGC 2 cut(s) 409, 809
Csp6I GTAC 4 cut(s) 376, 490, 627, 905
CviAII CATG 2 cut(s) 533, 1007
CviQI GTAC 4 cut(s) 376, 490, 627, 905
DdeI CTNAG 3 cut(s) 753, 797, 1049
DpnI GATC 4 cut(s) 196, 320, 705, 1047
DpnII GATC 4 cut(s) 194, 318, 703, 1045
DraI TTTAAA 1 cut(s) 1167
DraIII CACNNNGTG 1 cut(s) 384
EaeI YGGCCR 1 cut(s) 1009
Ecl136II GAGCTC 1 cut(s) 644
Eco130I CCWWGG 2 cut(s) 365, 1006
Eco24I GRGCYC 1 cut(s) 646
Eco53kI GAGCTC 1 cut(s) 644
Eco57I CTGAAG 1 cut(s) 795
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 217, 493
EcoT14I CCWWGG 2 cut(s) 365, 1006
EcoT38I GRGCYC 1 cut(s) 646
ErhI CCWWGG 2 cut(s) 365, 1006
Esp3I CGTCTC 1 cut(s) 827
FaeI CATG 2 cut(s) 536, 1010
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FatI CATG 2 cut(s) 532, 1006
FauNDI CATATG 1 cut(s) 760
FbaI TGATCA 2 cut(s) 318, 703
Fnu4HI GCNGC 2 cut(s) 20, 291
FokI GGATG 5 cut(s) 130, 340, 442, 768, 1055
FriOI GRGCYC 1 cut(s) 646
Fsp4HI GCNGC 2 cut(s) 20, 291
FspBI CTAG 3 cut(s) 245, 294, 342
GluI GCNGC 2 cut(s) 20, 291
GsuI CTGGAG 2 cut(s) 201, 630
HaeIII GGCC 2 cut(s) 677, 1011
HgaI GACGC 2 cut(s) 409, 809
Hin1II CATG 2 cut(s) 536, 1010
HincII GTYRAC 2 cut(s) 190, 1102
HindII GTYRAC 2 cut(s) 190, 1102
HinfI GANTC 5 cut(s) 157, 514, 943, 1078, 1190
HphI GGTGA 3 cut(s) 432, 767, 1017
Hpy166II GTNNAC 4 cut(s) 190, 378, 1025, 1102
Hpy188I TCNGA 2 cut(s) 156, 1050
Hpy8I GTNNAC 4 cut(s) 190, 378, 1025, 1102
Hpy99I CGWCG 1 cut(s) 448
HpyAV CCTTC 3 cut(s) 612, 690, 1103
HpyCH4III ACNGT 5 cut(s) 183, 257, 848, 899, 1090
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 4 cut(s) 235, 347, 865, 921
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 400
HpyF3I CTNAG 3 cut(s) 753, 797, 1049
HpySE526I ACGT 1 cut(s) 446
Hsp92II CATG 2 cut(s) 536, 1010
Ksp22I TGATCA 2 cut(s) 318, 703
Kzo9I GATC 4 cut(s) 194, 318, 703, 1045
LmnI GCTCC 2 cut(s) 220, 649
Lsp1109I GCAGC 2 cut(s) 6, 277
LweI GCATC 1 cut(s) 356
MaeI CTAG 3 cut(s) 245, 294, 342
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 5 cut(s) 51, 79, 397, 577, 926
MalI GATC 4 cut(s) 196, 320, 705, 1047
MboI GATC 4 cut(s) 194, 318, 703, 1045
MboII GAAGA 6 cut(s) 429, 554, 577, 764, 883, 950
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 646
MlsI TGGCCA 1 cut(s) 1011
MluCI AATT 3 cut(s) 299, 466, 503
MluNI TGGCCA 1 cut(s) 1011
MlyI GAGTC 2 cut(s) 523, 1087
MnlI CCTC 3 cut(s) 470, 667, 748
Mox20I TGGCCA 1 cut(s) 1011
MroXI GAANNNNTTC 1 cut(s) 175
MscI TGGCCA 1 cut(s) 1011
MseI TTAA 3 cut(s) 594, 1166, 1197
MslI CAYNNNNRTG 1 cut(s) 445
Msp20I TGGCCA 1 cut(s) 1011
MspR9I CCNGG 2 cut(s) 219, 495
Mva1269I GAATGC 2 cut(s) 396, 744
MvaI CCWGG 2 cut(s) 219, 495
MwoI GCNNNNNNNGC 2 cut(s) 28, 400
NcoI CCATGG 1 cut(s) 1006
NdeI CATATG 1 cut(s) 760
NdeII GATC 4 cut(s) 194, 318, 703, 1045
NlaIII CATG 2 cut(s) 536, 1010
NlaIV GGNNCC 4 cut(s) 126, 216, 678, 970
NmuCI GTSAC 4 cut(s) 79, 397, 577, 926
OliI CACNNNNGTG 1 cut(s) 445
PaeR7I CTCGAG 1 cut(s) 161
PctI GAATGC 2 cut(s) 396, 744
PdmI GAANNNNTTC 1 cut(s) 175
PfeI GAWTC 3 cut(s) 157, 943, 1190
PkrI GCNGC 2 cut(s) 21, 292
PleI GAGTC 2 cut(s) 522, 1086
PpsI GAGTC 2 cut(s) 522, 1086
Psp124BI GAGCTC 1 cut(s) 646
Psp6I CCWGG 2 cut(s) 217, 493
PspGI CCWGG 2 cut(s) 217, 493
PspN4I GGNNCC 4 cut(s) 126, 216, 678, 970
PspPI GGNCC 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 1061
PsuI RGATCY 1 cut(s) 194
RsaI GTAC 4 cut(s) 377, 491, 628, 906
RsaNI GTAC 4 cut(s) 376, 490, 627, 905
RseI CAYNNNNRTG 1 cut(s) 445
SacI GAGCTC 1 cut(s) 646
SaqAI TTAA 3 cut(s) 594, 1166, 1197
SatI GCNGC 2 cut(s) 20, 291
Sau3AI GATC 4 cut(s) 194, 318, 703, 1045
Sau96I GGNCC 1 cut(s) 676
SchI GAGTC 2 cut(s) 523, 1087
ScrFI CCNGG 2 cut(s) 219, 495
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 1 cut(s) 356
Sfr274I CTCGAG 1 cut(s) 161
SlaI CTCGAG 1 cut(s) 161
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 3 cut(s) 37, 161, 1147
SmoI CTYRAG 3 cut(s) 37, 161, 1147
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 3 cut(s) 299, 466, 503
SsiI CCGC 1 cut(s) 419
SspMI CTAG 3 cut(s) 245, 294, 342
SstI GAGCTC 1 cut(s) 646
StyD4I CCNGG 2 cut(s) 217, 493
StyI CCWWGG 2 cut(s) 365, 1006
TaaI ACNGT 5 cut(s) 183, 257, 848, 899, 1090
TaiI ACGT 1 cut(s) 449
TaqI TCGA 3 cut(s) 162, 324, 1076
TasI AATT 3 cut(s) 299, 466, 503
TatI WGTACW 2 cut(s) 375, 904
TfiI GAWTC 3 cut(s) 157, 943, 1190
Tru1I TTAA 3 cut(s) 594, 1166, 1197
Tru9I TTAA 3 cut(s) 594, 1166, 1197
TscAI CASTG 1 cut(s) 792
TseFI GTSAC 4 cut(s) 79, 397, 577, 926
TseI GCWGC 2 cut(s) 19, 290
Tsp45I GTSAC 4 cut(s) 79, 397, 577, 926
TspDTI ATGAA 2 cut(s) 516, 1122
TspGWI ACGGA 3 cut(s) 580, 1170, 1183
TspRI CASTG 1 cut(s) 792
XapI RAATTY 3 cut(s) 299, 466, 503
XhoI CTCGAG 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 175
XspI CTAG 3 cut(s) 245, 294, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.