AT5G56570

FBD-associated F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000140
Physical Location & Seq
Forward (+)
22902736 .. 22904596
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56570.1

Sequence Viewer

Length: 1320 bp
ATGAGAAAACTTTTCTTCTTCCTTTGCACACACGTCCCAAAACAGAGAGCAAAATTCACCAGAATCATGGAGAGCTGCCAGAAGAAACAAAAATTGGAGACACCAACAGAGCTCAGTGATATGCCTGATGACTTAATCTTCAAAATATTCTCGTTCCTTCCGTTTTTTAAAGAGGATTTAGCAACACGTTTCATATCAGAATATGGGAAGGGTCTTTGGAATCCAGACCCGAATGCCATATTTGATGATGAATCACTCGATATGACTTGTATGAGTTTTGTATATGGATCTTTGATGTCCAAGGATGCTCAGATTCTAGATAGCTTGCATCTCAAGCTTAGGAAATACTATATTGCTTCAGATATCAATTTTCTTGTTCAACTTGGGGTTAATCGATCTATGAGAGAGCTGAGAATCGACTTCTTTGGAAAAACCCTAGAATTACCGTGTTGCTTGAGTACCTGCACAACCCTAAAAGTTTTGGTACTCGATCATTTAAATATCATGTCTGTCCCGGGTTGGTTTCGTTTGCCATCAGTCGAAACTCTCCAGCTTTCATCGGTTACCGGGGGCAGCAACTATGTTCCAAGTCTTATAAGATTATGCTCTGTTCATGAACGTTTGGTTGTAGACCAAAACAGAAATAAAGATCTGGTTAATATCAATGTGCCTACTTTGAGGAGCTTATCTATCGATAACAAGAGAAGGGGACATGTGCCACTCGGTAGCTTTTGGATAAATGTTCCTTCTTTGATGTTTTTGAACATTAAGGATACATTCCAAACAATATTTGAATCTATGCCTGCGATGATCAAAGCGAATATCGAGGTTGCTCATGACCAATCGCAATCTGTGAAGTTCATAGAATCTCTTACCTCAACCCGACATCTTTGTCTTTGTTCTCCAACTTCAGAGAATCCATATCCTAATGGCACAAAGTTCTCCAATCTTGTACATCTAAAGCTATGTACATGTTCTGCGGGATGGCAGAATCTACTTGCGTGTATGCTCAATGACGCTCCCAATCTTCGTTCTCTTACTCTTAAGTTGAGACAAAAATCCGATGTCAATCCGAAGAAAGTCTGGGAAAAGCCAACAGTCGTTCCTGAATGTTTATCTACGCGTCTTGAGATCTTGAAATGGAGAGATTATGAAGGCACTGAGCATGAGAAGGATATGGTTGGGTACATACTAGCAAACGCTACTTTTCTACAGAGGGCAACATTCTCAACGAAAGATAGAAACCAATGTGATTCCAGGTTCAGCGAGTTACAGTCTATGGAGAGAGTTTCAGAGATATGTGAGTTTGTGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

439

Amino Acids

50.46

Weight (kDa)

8.28

Isoelectric Point (pI)

56.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 365 - 409 8.5e-17 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05080 AT1G19410 AT1G22000 AT1G26815 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G32375 AT1G32375 AT1G50980 AT1G51055 AT1G55030 AT1G55030 AT1G55660 AT1G55660 AT2G04230 AT2G26860 AT2G26860 AT3G10750 AT3G26920 AT3G26920 AT3G26920 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26930 AT3G26930 AT3G49020 AT3G49030 AT3G49030 AT3G49040 AT3G49045 AT3G49480 AT3G49480 AT3G50710 AT3G51530 AT3G52680 AT3G52680 AT3G52690 AT3G53550 AT3G54910 AT3G54910 AT3G54910 AT3G55670 AT4G00160 AT4G00315 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G10400 AT4G10400 AT4G10400 AT4G10410 AT4G10420 AT4G13965 AT4G15060 AT4G15060 AT4G15075 AT4G26340 AT4G26350 AT5G11370 AT5G38565 AT5G38570 AT5G38580 AT5G38590 AT5G38590 AT5G38590 AT5G38590 AT5G40750 AT5G44850 AT5G50270 AT5G50270 AT5G52460 AT5G52460 AT5G56325 AT5G56370 AT5G56370 AT5G56370 AT5G56380 AT5G56380 AT5G56390 AT5G56400 AT5G56410 AT5G56420 AT5G56420 AT5G56420 AT5G56430 AT5G56440 AT5G56452 AT5G56452 AT5G56555 AT5G56560 AT5G56560 AT5G56570 AT5G56690 AT5G56700 AT5G56700 AT5G56800 AT5G56810 AT5G56810 AT5G56820 AT5G60610 AT5G60610 AT5G62970
malus_domestica MD10G1316000.v1.1
prunus_persica Prupe.4G024600_v2.0.a1
pyrus_communis pycom10g26820
rosa_chinensis RchiOBHm_Chr1g0383101 RchiOBHm_Chr5g0003811 RchiOBHm_Chr5g0069761 RchiOBHm_Chr5g0069771 RchiOBHm_Chr5g0069781 RchiOBHm_Chr5g0069791 RchiOBHm_Chr5g0069821 RchiOBHm_Chr5g0069831
rosa_laevigata RLG00000026130 RLG00000026375 RLG00000031193 RLG00000036088 RLG00000036089 RLG00000036090 RLG00000036095 RLG00000036096 RLG00000036097 RLG00000036099 RLG00000036100
rosa_multiflora Rmu_co8192842.1_g000001 Rmu_co8209784.1_g000001 Rmu_sc0000726.1_g000001 Rmu_sc0000726.1_g000003 Rmu_sc0000726.1_g000007 Rmu_sc0000726.1_g000008 Rmu_sc0000738.1_g000009 Rmu_sc0000738.1_g000010 Rmu_sc0000738.1_g000011 Rmu_sc0000738.1_g000015 Rmu_sc0000738.1_g000018 Rmu_sc0000738.1_g000019 Rmu_sc0004212.1_g000009 Rmu_sc0017283.1_g000003 Rmu_sc0024481.1_g000001
rosa_roxburghii Rroxscaffold_1G00011240 Rroxscaffold_1G00011270 Rroxscaffold_1G00011280 Rroxscaffold_1G00011300 Rroxscaffold_1G00011310 Rroxscaffold_1G00011320 Rroxscaffold_4G00277590
rosa_rugosa Rorug01G0413100 Rorug01G0438500 Rorug01G0438600 Rorug04G0406800 Rorug05G0399300 Rorug05G0399400 Rorug05G0399500 Rorug05G0399600 Rorug05G0399700 Rorug05G0399900.1 Rorug05G0400000 Rorug05G0400100
rosa_samantha Rh1AG436100 Rh1AG464200 Rh1BG421500 Rh1CG434900 Rh1DG454100 Rh5AG034000 Rh5AG034100 Rh5AG456400 Rh5AG456500 Rh5AG456600 Rh5AG456800 Rh5AG457000 Rh5BG032600 Rh5BG475500 Rh5BG475600 Rh5BG475800 Rh5BG475900 Rh5CG036600 Rh5CG036700 Rh5CG499100 Rh5CG499200 Rh5CG499400 Rh5CG499600 Rh5DG032800 Rh5DG487000 Rh5DG487100 Rh5DG487200 Rh5DG487400 Rh5DG487500
rosa_wichuraiana Rw5G003130 Rw5G042610 Rw5G042630 Rw5G042650 Rw5G042660 Rw5G042670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
AasI GACNNNNNNGTC 1 cut(s) 891
Acc36I ACCTGC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 630
AccII CGCG 1 cut(s) 1123
AciI CCGC 1 cut(s) 980
AclI AACGTT 1 cut(s) 619
AclWI GGATC 1 cut(s) 295
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 2 cut(s) 342, 894
AfaI GTAC 5 cut(s) 460, 486, 954, 970, 1187
AflII CTTAAG 1 cut(s) 1043
AflIII ACRYGT 5 cut(s) 31, 185, 712, 971, 1121
AgsI TTSAA 5 cut(s) 142, 380, 763, 794, 1138
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 1 cut(s) 1256
AjuI GAANNNNNNNTTGG 2 cut(s) 77, 109
AluBI AGCT 9 cut(s) 75, 112, 324, 337, 409, 553, 684, 729, 964
AluI AGCT 9 cut(s) 75, 112, 324, 337, 409, 553, 684, 729, 964
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 2 cut(s) 92, 1045
AlwI GGATC 1 cut(s) 295
Ama87I CYCGRG 1 cut(s) 514
ApeKI GCWGC 2 cut(s) 75, 573
ApoI RAATTY 1 cut(s) 53
AsuC2I CCSGG 3 cut(s) 515, 516, 568
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 514
BanII GRGCYC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 62, 585
BccI CCATC 2 cut(s) 541, 978
BciT130I CCWGG 1 cut(s) 1258
BciVI GTATCC 1 cut(s) 766
BclI TGATCA 1 cut(s) 810
BcnI CCSGG 3 cut(s) 515, 516, 568
BcoDI GTCTC 2 cut(s) 92, 1045
BfaI CTAG 3 cut(s) 317, 437, 1193
BfmI CTRYAG 1 cut(s) 1211
BfrI CTTAAG 1 cut(s) 1043
BfuAI ACCTGC 1 cut(s) 470
BfuI GTATCC 1 cut(s) 766
BglII AGATCT 2 cut(s) 649, 1131
BisI GCNGC 2 cut(s) 76, 574
BlsI GCNGC 2 cut(s) 77, 575
Bme1390I CCNGG 4 cut(s) 515, 516, 568, 1258
BmeT110I CYCGRG 1 cut(s) 514
BmgBI CACGTC 1 cut(s) 34
BmrFI CCNGG 4 cut(s) 515, 516, 568, 1258
BmsI GCATC 2 cut(s) 295, 337
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 3 cut(s) 317, 475, 1148
BpuMI CCSGG 3 cut(s) 515, 516, 568
Bsa29I ATCGAT 2 cut(s) 394, 693
BsaBI GATNNNNATC 1 cut(s) 1068
BsaJI CCNNGG 3 cut(s) 300, 514, 567
Bse8I GATNNNNATC 1 cut(s) 1068
BseBI CCWGG 1 cut(s) 1258
BseCI ATCGAT 2 cut(s) 394, 693
BseDI CCNNGG 3 cut(s) 300, 514, 567
BseGI GGATG 2 cut(s) 310, 989
BseJI GATNNNNATC 1 cut(s) 1068
BseMII CTCAG 4 cut(s) 127, 323, 401, 1152
BseRI GAGGAG 1 cut(s) 694
BseXI GCAGC 2 cut(s) 62, 585
BsgI GTGCAG 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 1123
BshVI ATCGAT 2 cut(s) 394, 693
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 2 cut(s) 515, 567
BslFI GGGAC 3 cut(s) 20, 497, 723
BsmAI GTCTC 2 cut(s) 92, 1045
BsmFI GGGAC 3 cut(s) 20, 497, 723
BsmI GAATGC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 1 cut(s) 114
Bsp1407I TGTACA 2 cut(s) 952, 968
Bsp143I GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
BspACI CCGC 1 cut(s) 980
BspCNI CTCAG 4 cut(s) 126, 322, 402, 1153
BspDI ATCGAT 2 cut(s) 394, 693
BspFNI CGCG 1 cut(s) 1123
BspHI TCATGA 2 cut(s) 613, 835
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 1 cut(s) 295
BspTI CTTAAG 1 cut(s) 1043
BsrGI TGTACA 2 cut(s) 952, 968
BssECI CCNNGG 3 cut(s) 300, 514, 567
BssMI GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 1258
Bst4CI ACNGT 3 cut(s) 447, 1099, 1275
BstAFI CTTAAG 1 cut(s) 1043
BstAUI TGTACA 2 cut(s) 952, 968
BstC8I GCNNGC 2 cut(s) 326, 804
BstDEI CTNAG 5 cut(s) 113, 309, 338, 410, 1161
BstEII GGTNACC 1 cut(s) 562
BstF5I GGATG 2 cut(s) 310, 989
BstFNI CGCG 1 cut(s) 1123
BstKTI GATC 6 cut(s) 290, 398, 493, 652, 813, 1134
BstMAI GTCTC 2 cut(s) 92, 1045
BstMBI GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNI CCWGG 1 cut(s) 1258
BstNSI RCATGY 2 cut(s) 716, 975
BstPI GGTNACC 1 cut(s) 562
BstSCI CCNGG 4 cut(s) 513, 514, 566, 1256
BstSFI CTRYAG 1 cut(s) 1211
BstUI CGCG 1 cut(s) 1123
BstV1I GCAGC 2 cut(s) 62, 585
BstX2I RGATCY 3 cut(s) 287, 649, 1131
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 3 cut(s) 287, 649, 1131
Bsu15I ATCGAT 2 cut(s) 394, 693
BsuI GTATCC 1 cut(s) 766
BsuTUI ATCGAT 2 cut(s) 394, 693
BtgZI GCGATG 1 cut(s) 821
BtrI CACGTC 1 cut(s) 34
BtsCI GGATG 2 cut(s) 310, 989
BtsIMutI CAGTG 2 cut(s) 121, 1158
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 2 cut(s) 326, 804
CciI TCATGA 2 cut(s) 613, 835
Cfr9I CCCGGG 1 cut(s) 514
ClaI ATCGAT 2 cut(s) 394, 693
CseI GACGC 2 cut(s) 1025, 1112
Csp6I GTAC 5 cut(s) 459, 485, 953, 969, 1186
CviAII CATG 7 cut(s) 67, 505, 614, 713, 836, 972, 1166
CviQI GTAC 5 cut(s) 459, 485, 953, 969, 1186
DdeI CTNAG 5 cut(s) 113, 309, 338, 410, 1161
DpnI GATC 6 cut(s) 289, 397, 492, 651, 812, 1133
DpnII GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
DraI TTTAAA 2 cut(s) 169, 498
DrdI GACNNNNNNGTC 1 cut(s) 891
DseDI GACNNNNNNGTC 1 cut(s) 891
Ecl136II GAGCTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 114
Eco32I GATATC 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 112
Eco57I CTGAAG 2 cut(s) 342, 894
Eco88I CYCGRG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 562
EcoICRI GAGCTC 1 cut(s) 112
EcoO65I GGTNACC 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 1256
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 7 cut(s) 70, 508, 617, 716, 839, 975, 1169
FaqI GGGAC 3 cut(s) 20, 497, 723
FatI CATG 7 cut(s) 66, 504, 613, 712, 835, 971, 1165
FauI CCCGC 1 cut(s) 973
FbaI TGATCA 1 cut(s) 810
FblI GTMKAC 1 cut(s) 630
Fnu4HI GCNGC 2 cut(s) 76, 574
FokI GGATG 2 cut(s) 317, 996
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 2 cut(s) 76, 574
FspBI CTAG 3 cut(s) 317, 437, 1193
GluI GCNGC 2 cut(s) 76, 574
GsuI CTGGAG 1 cut(s) 533
HapII CCGG 2 cut(s) 515, 567
HgaI GACGC 2 cut(s) 1025, 1112
Hin1II CATG 7 cut(s) 70, 508, 617, 716, 839, 975, 1169
HindIII AAGCTT 1 cut(s) 335
HpaII CCGG 2 cut(s) 515, 567
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 7 cut(s) 199, 312, 361, 913, 1063, 1074, 1294
Hpy188III TCNNGA 7 cut(s) 224, 317, 614, 836, 1106, 1127, 1135
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 6 cut(s) 167, 202, 699, 756, 1148, 1165
HpyCH4III ACNGT 3 cut(s) 447, 1099, 1275
HpyCH4IV ACGT 3 cut(s) 33, 187, 619
HpyCH4V TGCA 3 cut(s) 27, 328, 465
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 5 cut(s) 113, 309, 338, 410, 1161
HpySE526I ACGT 3 cut(s) 33, 187, 619
Hsp92II CATG 7 cut(s) 70, 508, 617, 716, 839, 975, 1169
Ksp22I TGATCA 1 cut(s) 810
Kzo9I GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
LmnI GCTCC 2 cut(s) 681, 1024
Lsp1109I GCAGC 2 cut(s) 62, 585
LweI GCATC 2 cut(s) 295, 337
MaeI CTAG 3 cut(s) 317, 437, 1193
MaeII ACGT 3 cut(s) 33, 187, 619
MaeIII GTNAC 2 cut(s) 562, 1269
MalI GATC 6 cut(s) 289, 397, 492, 651, 812, 1133
MboI GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
MboII GAAGA 6 cut(s) 7, 10, 94, 130, 1019, 1087
MflI RGATCY 3 cut(s) 287, 649, 1131
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 4 cut(s) 53, 92, 367, 440
MluI ACGCGT 1 cut(s) 1121
MmeI TCCRAC 1 cut(s) 929
MnlI CCTC 5 cut(s) 166, 672, 820, 886, 1209
MseI TTAA 7 cut(s) 134, 168, 390, 497, 657, 768, 1044
MspCI CTTAAG 1 cut(s) 1043
MspI CCGG 2 cut(s) 515, 567
MspR9I CCNGG 4 cut(s) 515, 516, 568, 1258
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 1258
MvnI CGCG 1 cut(s) 1123
MwoI GCNNNNNNNGC 1 cut(s) 334
NciI CCSGG 3 cut(s) 515, 516, 568
NdeII GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
NlaIII CATG 7 cut(s) 70, 508, 617, 716, 839, 975, 1169
NspI RCATGY 2 cut(s) 716, 975
PagI TCATGA 2 cut(s) 613, 835
PciI ACATGT 2 cut(s) 712, 971
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PctI GAATGC 1 cut(s) 238
PkrI GCNGC 2 cut(s) 77, 575
PscI ACATGT 2 cut(s) 712, 971
PsiI TTATAA 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 114
Psp1406I AACGTT 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 1256
PspEI GGTNACC 1 cut(s) 562
PspGI CCWGG 1 cut(s) 1256
PsuI RGATCY 3 cut(s) 287, 649, 1131
RsaI GTAC 5 cut(s) 460, 486, 954, 970, 1187
RsaNI GTAC 5 cut(s) 459, 485, 953, 969, 1186
SacI GAGCTC 1 cut(s) 114
SaqAI TTAA 7 cut(s) 134, 168, 390, 497, 657, 768, 1044
SatI GCNGC 2 cut(s) 76, 574
Sau3AI GATC 6 cut(s) 287, 395, 490, 649, 810, 1131
ScrFI CCNGG 4 cut(s) 515, 516, 568, 1258
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 2 cut(s) 295, 337
SfcI CTRYAG 1 cut(s) 1211
SmaI CCCGGG 1 cut(s) 516
SmiI ATTTAAAT 1 cut(s) 498
SmlI CTYRAG 4 cut(s) 332, 454, 1043, 1127
SmoI CTYRAG 4 cut(s) 332, 454, 1043, 1127
Sse9I AATT 4 cut(s) 53, 92, 367, 440
SsiI CCGC 1 cut(s) 980
SspI AATATT 2 cut(s) 147, 789
SspMI CTAG 3 cut(s) 317, 437, 1193
SstI GAGCTC 1 cut(s) 114
StyD4I CCNGG 4 cut(s) 513, 514, 566, 1256
StyI CCWWGG 1 cut(s) 300
SwaI ATTTAAAT 1 cut(s) 498
TaaI ACNGT 3 cut(s) 447, 1099, 1275
TaiI ACGT 3 cut(s) 36, 190, 622
TaqI TCGA 7 cut(s) 258, 394, 417, 489, 540, 693, 825
TasI AATT 4 cut(s) 53, 92, 367, 440
TatI WGTACW 2 cut(s) 952, 968
Tru1I TTAA 7 cut(s) 134, 168, 390, 497, 657, 768, 1044
Tru9I TTAA 7 cut(s) 134, 168, 390, 497, 657, 768, 1044
TscAI CASTG 2 cut(s) 121, 1165
TseI GCWGC 2 cut(s) 75, 573
TspDTI ATGAA 7 cut(s) 181, 264, 546, 602, 630, 850, 1167
TspGWI ACGGA 1 cut(s) 150
TspMI CCCGGG 1 cut(s) 514
TspRI CASTG 2 cut(s) 121, 1165
Vha464I CTTAAG 1 cut(s) 1043
XapI RAATTY 1 cut(s) 53
XbaI TCTAGA 1 cut(s) 316
XceI RCATGY 2 cut(s) 716, 975
XmaI CCCGGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 3 cut(s) 317, 437, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.