Rh5BG475600

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000140
Physical Location & Seq
Forward (+)
75721561 .. 75726279
4719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG475600.1

Sequence Viewer

Length: 1392 bp
ATGGATACCAATTCTCGGATAGTAAAACGCGAAAAACAAAAGTTGGATGGAGGAGATGAGATTGCAGGAGGCAGCAAGAGTTTTGGCAATTTACCTGAGGAACTTATTAAATACATTCTGTCCTTGCTTCCAACAGAAGATCTTATCAAAACAAGCGTTTTGTGTAAAAAGTGGGAGTACCTATGGACATCGTTTTCCAAGTTTAGTTTTGTTCAAGGGTCGTCTGATGATAATAGATCTTCTTTCTTGAAAGCTGTGGATAGAGCACTTATTCTTCGGGATGCAGATATAGAACAGTTCAGTGTTTCGTTTACAGTGAAGAAGGGTGATGCATCTCGTGTTAGTTCGTGGACTTCTGGCTTAGTCTGGCGCAACGTTGAAAAGCTTTCCCTTGCTCTTGATGGGGCTAGGTCAACATTTTCGTTGGCTCCGTGTTTATTTACTTGTGCGACACTGAGGGACTTAGAACTATATATCCCTAGTAAATTGAAAGTTCCTGCTACTGTTTGTTTCCCATGTCTAAGGAGATTGTCTCTGAACTCTGTGGTGTTTACTGATGATTATTCAACTCAAAAGCTGTTTTCTGGTTGCCCAGTTCTCAATGAATTATCTCTAATCAATTGCAATTGGGCGAAACTCAAGTGTGTGACTATTTCTGCTTCCAATCTTTTGAGCTTGATCATATATGAAACTGGTGGTCAGTTGCAGCTTTTCGGACGTTGTTGTCAGATTATGGTAAATGGAGTCGGTCTCAAATATTTTCAGTACAAGGGCGGGTTCCTAAATGATTATTACTTTTATAACTCATCGGTAGATAAGGCTGAGATTCTTATTCCTACTTCGAGATTGAGACATGTGAGTTACCGGTTGTATAAGCTGCTTCGGGAGTTCTCTAGTATGAAAATTTTTACTACCTCTTCTTCATCCTTTAAGGTGGTTCAGACTAATGCATCAGAACTTCTCGCCCAGATGCCCTTGTTTAGTAATCTAGCCACTTTGATATTTGAAGAAAAGGAAGTAAATATTGGTTGTAAGGGACTACTGAGGATGTTCCAAAACTGTCCTTCTCTCCAAACTCTAGTATGCAGTGAGGGCATTATGCTGTCCTCCGATGATGCAGAAGATGATGGGGTGTTAGAGCCACTGCCTCCTTGTTTCTTGAAAAGTCTTAAAAAGATTGAAGTTCATGCGTTTCTTGGAAACCAGGAAGAGCTTGATGCATTAAAGGTTCTGTTAAAGAATGCAATGGTCTTGGAGAAGCTGATTATAACTTGCTCCGATTCTTTTCAAGGCAGACCGGAGAAGTTGAGGGATGTTAACAATCAGTTAGCAGATTTACCTAGAGGCTCATCCAAATGCTGTGAAGTTATTTGTTCGTTGAGCCCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

463

Amino Acids

51.74

Weight (kDa)

8.18

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 30 - 68 1.6e-09 F-box domain
LRR_At5g56370 PF24758 115 - 230 1.6e-13 FBD-associated F-box protein At5g56370, LRR repeats
FBD PF08387 382 - 423 2.7e-09 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05080 AT1G19410 AT1G22000 AT1G26815 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G26890 AT1G32375 AT1G32375 AT1G50980 AT1G51055 AT1G55030 AT1G55030 AT1G55660 AT1G55660 AT2G04230 AT2G26860 AT2G26860 AT3G10750 AT3G26920 AT3G26920 AT3G26920 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26922 AT3G26930 AT3G26930 AT3G49020 AT3G49030 AT3G49030 AT3G49040 AT3G49045 AT3G49480 AT3G49480 AT3G50710 AT3G51530 AT3G52680 AT3G52680 AT3G52690 AT3G53550 AT3G54910 AT3G54910 AT3G54910 AT3G55670 AT4G00160 AT4G00315 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G09920 AT4G10400 AT4G10400 AT4G10400 AT4G10410 AT4G10420 AT4G13965 AT4G15060 AT4G15060 AT4G15075 AT4G26340 AT4G26350 AT5G11370 AT5G38565 AT5G38570 AT5G38580 AT5G38590 AT5G38590 AT5G38590 AT5G38590 AT5G40750 AT5G44850 AT5G50270 AT5G50270 AT5G52460 AT5G52460 AT5G56325 AT5G56370 AT5G56370 AT5G56370 AT5G56380 AT5G56380 AT5G56390 AT5G56400 AT5G56410 AT5G56420 AT5G56420 AT5G56420 AT5G56430 AT5G56440 AT5G56452 AT5G56452 AT5G56555 AT5G56560 AT5G56560 AT5G56570 AT5G56690 AT5G56700 AT5G56700 AT5G56800 AT5G56810 AT5G56810 AT5G56820 AT5G60610 AT5G60610 AT5G62970
malus_domestica MD10G1316000.v1.1
prunus_persica Prupe.4G024600_v2.0.a1
pyrus_communis pycom10g26820
rosa_chinensis RchiOBHm_Chr1g0383101 RchiOBHm_Chr5g0003811 RchiOBHm_Chr5g0069761 RchiOBHm_Chr5g0069771 RchiOBHm_Chr5g0069781 RchiOBHm_Chr5g0069791 RchiOBHm_Chr5g0069821 RchiOBHm_Chr5g0069831
rosa_laevigata RLG00000026130 RLG00000026375 RLG00000031193 RLG00000036088 RLG00000036089 RLG00000036090 RLG00000036095 RLG00000036096 RLG00000036097 RLG00000036099 RLG00000036100
rosa_multiflora Rmu_co8192842.1_g000001 Rmu_co8209784.1_g000001 Rmu_sc0000726.1_g000001 Rmu_sc0000726.1_g000003 Rmu_sc0000726.1_g000007 Rmu_sc0000726.1_g000008 Rmu_sc0000738.1_g000009 Rmu_sc0000738.1_g000010 Rmu_sc0000738.1_g000011 Rmu_sc0000738.1_g000015 Rmu_sc0000738.1_g000018 Rmu_sc0000738.1_g000019 Rmu_sc0004212.1_g000009 Rmu_sc0017283.1_g000003 Rmu_sc0024481.1_g000001
rosa_roxburghii Rroxscaffold_1G00011240 Rroxscaffold_1G00011270 Rroxscaffold_1G00011280 Rroxscaffold_1G00011300 Rroxscaffold_1G00011310 Rroxscaffold_1G00011320 Rroxscaffold_4G00277590
rosa_rugosa Rorug01G0413100 Rorug01G0438500 Rorug01G0438600 Rorug04G0406800 Rorug05G0399300 Rorug05G0399400 Rorug05G0399500 Rorug05G0399600 Rorug05G0399700 Rorug05G0399900.1 Rorug05G0400000 Rorug05G0400100
rosa_samantha Rh1AG436100 Rh1AG464200 Rh1BG421500 Rh1CG434900 Rh1DG454100 Rh5AG034000 Rh5AG034100 Rh5AG456400 Rh5AG456500 Rh5AG456600 Rh5AG456800 Rh5AG457000 Rh5BG032600 Rh5BG475500 Rh5BG475600 Rh5BG475800 Rh5BG475900 Rh5CG036600 Rh5CG036700 Rh5CG499100 Rh5CG499200 Rh5CG499400 Rh5CG499600 Rh5DG032800 Rh5DG487000 Rh5DG487100 Rh5DG487200 Rh5DG487400 Rh5DG487500
rosa_wichuraiana Rw5G003130 Rw5G042610 Rw5G042630 Rw5G042650 Rw5G042660 Rw5G042670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 801, 1268
AasI GACNNNNNNGTC 1 cut(s) 723
AccII CGCG 1 cut(s) 30
AciI CCGC 1 cut(s) 774
AclI AACGTT 1 cut(s) 375
AcsI RAATTY 1 cut(s) 903
AfaI GTAC 2 cut(s) 179, 767
AfiI CCNNNNNNNGG 1 cut(s) 15
AflIII ACRYGT 1 cut(s) 853
AgeI ACCGGT 1 cut(s) 864
AgsI TTSAA 9 cut(s) 215, 250, 380, 490, 567, 1007, 1162, 1181, 1289
AjnI CCWGG 1 cut(s) 1203
AjuI GAANNNNNNNTTGG 2 cut(s) 1008, 1040
AloI GAACNNNNNNTCC 2 cut(s) 459, 491
AluBI AGCT 8 cut(s) 254, 385, 577, 675, 709, 877, 1213, 1261
AluI AGCT 8 cut(s) 254, 385, 577, 675, 709, 877, 1213, 1261
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 3 cut(s) 537, 755, 844
ApeKI GCWGC 3 cut(s) 72, 706, 877
ApoI RAATTY 1 cut(s) 903
AsiGI ACCGGT 1 cut(s) 864
AspLEI GCGC 1 cut(s) 372
AsuHPI GGTGA 1 cut(s) 338
AxyI CCTNAGG 1 cut(s) 96
BanII GRGCYC 1 cut(s) 1385
BauI CACGAG 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 3 cut(s) 84, 718, 864
BccI CCATC 3 cut(s) 41, 395, 1121
BciT130I CCWGG 1 cut(s) 1205
BclI TGATCA 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 537, 755, 844
BfaI CTAG 6 cut(s) 408, 480, 894, 989, 1079, 1341
BglII AGATCT 2 cut(s) 139, 236
BisI GCNGC 3 cut(s) 73, 707, 878
BlsI GCNGC 3 cut(s) 74, 708, 879
Bme1390I CCNGG 1 cut(s) 1205
BmiI GGNNCC 2 cut(s) 429, 779
BmrFI CCNGG 1 cut(s) 1205
BmrI ACTGGG 1 cut(s) 587
BmsI GCATC 7 cut(s) 271, 319, 341, 959, 960, 1105, 1207
BmuI ACTGGG 1 cut(s) 587
BplI GAGNNNNNCTC 4 cut(s) 517, 549, 735, 767
BpuEI CTTGAG 1 cut(s) 623
BsaI GGTCTC 1 cut(s) 755
BsaWI WCCGGW 2 cut(s) 864, 1297
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 1 cut(s) 864
Bse1I ACTGG 2 cut(s) 593, 697
Bse21I CCTNAGG 1 cut(s) 96
Bse3DI GCAATG 1 cut(s) 1251
BseBI CCWGG 1 cut(s) 1205
BseGI GGATG 6 cut(s) 52, 286, 923, 1053, 1318, 1349
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMI GCAATG 1 cut(s) 1251
BseMII CTCAG 4 cut(s) 87, 446, 813, 1034
BseNI ACTGG 2 cut(s) 593, 697
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 3 cut(s) 84, 718, 864
Bsh1236I CGCG 1 cut(s) 30
BshTI ACCGGT 1 cut(s) 864
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 2 cut(s) 865, 1298
BslFI GGGAC 2 cut(s) 473, 1050
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 3 cut(s) 537, 755, 844
BsmFI GGGAC 2 cut(s) 473, 1050
BsmI GAATGC 1 cut(s) 1246
Bso31I GGTCTC 1 cut(s) 755
Bsp1286I GDGCHC 2 cut(s) 268, 1385
Bsp143I GATC 3 cut(s) 139, 236, 678
BspACI CCGC 1 cut(s) 774
BspCNI CTCAG 4 cut(s) 88, 447, 814, 1035
BspFNI CGCG 1 cut(s) 30
BspHI TCATGA 1 cut(s) 1388
BspLI GGNNCC 2 cut(s) 429, 779
BspQI GCTCTTC 1 cut(s) 1203
BspTNI GGTCTC 1 cut(s) 755
BsrDI GCAATG 1 cut(s) 1251
BsrFI RCCGGY 1 cut(s) 864
BsrI ACTGG 2 cut(s) 593, 697
BssAI RCCGGY 1 cut(s) 864
BssMI GATC 3 cut(s) 139, 236, 678
BssSI CACGAG 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 1205
Bst4CI ACNGT 4 cut(s) 297, 316, 505, 1061
Bst6I CTCTTC 2 cut(s) 922, 1203
BstDEI CTNAG 7 cut(s) 96, 361, 455, 463, 521, 822, 1043
BstF5I GGATG 6 cut(s) 52, 286, 923, 1053, 1318, 1349
BstFNI CGCG 1 cut(s) 30
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 3 cut(s) 142, 239, 681
BstMAI GTCTC 3 cut(s) 537, 755, 844
BstMBI GATC 3 cut(s) 139, 236, 678
BstMWI GCNNNNNNNGC 1 cut(s) 1092
BstNI CCWGG 1 cut(s) 1205
BstNSI RCATGY 1 cut(s) 857
BstSCI CCNGG 1 cut(s) 1203
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 84, 718, 864
BstX2I RGATCY 2 cut(s) 139, 236
BstYI RGATCY 2 cut(s) 139, 236
Bsu36I CCTNAGG 1 cut(s) 96
BtsCI GGATG 6 cut(s) 52, 286, 923, 1053, 1318, 1349
BtsI GCAGTG 2 cut(s) 1093, 1142
BtsIMutI CAGTG 5 cut(s) 307, 321, 452, 1093, 1142
CciI TCATGA 1 cut(s) 1388
CfoI GCGC 1 cut(s) 372
Cfr10I RCCGGY 1 cut(s) 864
Csp6I GTAC 2 cut(s) 178, 766
CspAI ACCGGT 1 cut(s) 864
CviAII CATG 4 cut(s) 516, 854, 1187, 1389
CviQI GTAC 2 cut(s) 178, 766
DdeI CTNAG 7 cut(s) 96, 361, 455, 463, 521, 822, 1043
DpnI GATC 3 cut(s) 141, 238, 680
DpnII GATC 3 cut(s) 139, 236, 678
DrdI GACNNNNNNGTC 1 cut(s) 723
DseDI GACNNNNNNGTC 1 cut(s) 723
Eam1104I CTCTTC 2 cut(s) 922, 1203
EarI CTCTTC 2 cut(s) 922, 1203
Eco24I GRGCYC 1 cut(s) 1385
Eco31I GGTCTC 1 cut(s) 755
Eco81I CCTNAGG 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 1203
EcoT22I ATGCAT 3 cut(s) 334, 952, 1222
EcoT38I GRGCYC 1 cut(s) 1385
FaeI CATG 4 cut(s) 519, 857, 1190, 1392
FaqI GGGAC 2 cut(s) 473, 1050
FatI CATG 4 cut(s) 515, 853, 1186, 1388
FauI CCCGC 1 cut(s) 767
FbaI TGATCA 1 cut(s) 678
Fnu4HI GCNGC 3 cut(s) 73, 707, 878
FokI GGATG 6 cut(s) 59, 293, 910, 1060, 1325, 1336
FriOI GRGCYC 1 cut(s) 1385
Fsp4HI GCNGC 3 cut(s) 73, 707, 878
FspBI CTAG 6 cut(s) 408, 480, 894, 989, 1079, 1341
GlaI GCGC 1 cut(s) 371
GluI GCNGC 3 cut(s) 73, 707, 878
HapII CCGG 2 cut(s) 865, 1298
HhaI GCGC 1 cut(s) 372
Hin1II CATG 4 cut(s) 519, 857, 1190, 1392
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 2 cut(s) 414, 1318
HindII GTYRAC 2 cut(s) 414, 1318
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 744, 826, 1280
HpaI GTTAAC 1 cut(s) 1318
HpaII CCGG 2 cut(s) 865, 1298
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 5 cut(s) 312, 351, 414, 552, 1318
Hpy188I TCNGA 9 cut(s) 18, 226, 537, 716, 729, 942, 955, 1111, 1279
Hpy188III TCNNGA 7 cut(s) 247, 278, 398, 843, 884, 1159, 1389
Hpy8I GTNNAC 5 cut(s) 312, 351, 414, 552, 1318
HpyAV CCTTC 2 cut(s) 316, 1074
HpyCH4III ACNGT 4 cut(s) 297, 316, 505, 1061
HpyCH4IV ACGT 2 cut(s) 375, 718
HpyF10VI GCNNNNNNNGC 1 cut(s) 1092
HpyF3I CTNAG 7 cut(s) 96, 361, 455, 463, 521, 822, 1043
HpySE526I ACGT 2 cut(s) 375, 718
Hsp92II CATG 4 cut(s) 519, 857, 1190, 1392
HspAI GCGC 1 cut(s) 370
Ksp22I TGATCA 1 cut(s) 678
KspAI GTTAAC 1 cut(s) 1318
Kzo9I GATC 3 cut(s) 139, 236, 678
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 2 cut(s) 433, 1280
Lsp1109I GCAGC 3 cut(s) 84, 718, 864
LweI GCATC 7 cut(s) 271, 319, 341, 959, 960, 1105, 1207
MaeI CTAG 6 cut(s) 408, 480, 894, 989, 1079, 1341
MaeII ACGT 2 cut(s) 375, 718
MaeIII GTNAC 2 cut(s) 646, 860
MalI GATC 3 cut(s) 141, 238, 680
MboI GATC 3 cut(s) 139, 236, 678
MboII GAAGA 9 cut(s) 149, 231, 266, 331, 909, 912, 1019, 1133, 1220
MfeI CAATTG 2 cut(s) 619, 625
MflI RGATCY 2 cut(s) 139, 236
MhlI GDGCHC 2 cut(s) 268, 1385
MluCI AATT 7 cut(s) 10, 88, 485, 605, 619, 625, 903
MlyI GAGTC 1 cut(s) 753
MmeI TCCRAC 2 cut(s) 24, 155
Mph1103I ATGCAT 3 cut(s) 334, 952, 1222
MseI TTAA 6 cut(s) 108, 930, 1170, 1223, 1235, 1317
MslI CAYNNNNRTG 1 cut(s) 1354
MspI CCGG 2 cut(s) 865, 1298
MspR9I CCNGG 1 cut(s) 1205
MunI CAATTG 2 cut(s) 619, 625
Mva1269I GAATGC 1 cut(s) 1246
MvaI CCWGG 1 cut(s) 1205
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 1092
NdeII GATC 3 cut(s) 139, 236, 678
NlaIII CATG 4 cut(s) 519, 857, 1190, 1392
NlaIV GGNNCC 2 cut(s) 429, 779
NmuCI GTSAC 1 cut(s) 646
NsiI ATGCAT 3 cut(s) 334, 952, 1222
NspI RCATGY 1 cut(s) 857
PagI TCATGA 1 cut(s) 1388
PciI ACATGT 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 1 cut(s) 428
PctI GAATGC 1 cut(s) 1246
PfeI GAWTC 2 cut(s) 826, 1280
PinAI ACCGGT 1 cut(s) 864
PkrI GCNGC 3 cut(s) 74, 708, 879
PleI GAGTC 1 cut(s) 752
PpsI GAGTC 1 cut(s) 752
PscI ACATGT 1 cut(s) 853
PsiI TTATAA 2 cut(s) 801, 1268
Psp1406I AACGTT 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 1203
PspGI CCWGG 1 cut(s) 1203
PspN4I GGNNCC 2 cut(s) 429, 779
PsuI RGATCY 2 cut(s) 139, 236
RsaI GTAC 2 cut(s) 179, 767
RsaNI GTAC 2 cut(s) 178, 766
RseI CAYNNNNRTG 1 cut(s) 1354
SapI GCTCTTC 1 cut(s) 1203
SaqAI TTAA 6 cut(s) 108, 930, 1170, 1223, 1235, 1317
SatI GCNGC 3 cut(s) 73, 707, 878
Sau3AI GATC 3 cut(s) 139, 236, 678
SchI GAGTC 1 cut(s) 753
ScrFI CCNGG 1 cut(s) 1205
SduI GDGCHC 2 cut(s) 268, 1385
SfaNI GCATC 7 cut(s) 271, 319, 341, 959, 960, 1105, 1207
SmiMI CAYNNNNRTG 1 cut(s) 1354
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 7 cut(s) 10, 88, 485, 605, 619, 625, 903
SsiI CCGC 1 cut(s) 774
SspI AATATT 2 cut(s) 758, 1024
SspMI CTAG 6 cut(s) 408, 480, 894, 989, 1079, 1341
StyD4I CCNGG 1 cut(s) 1203
TaaI ACNGT 4 cut(s) 297, 316, 505, 1061
TaiI ACGT 2 cut(s) 378, 721
TaqI TCGA 1 cut(s) 842
TaqII GACCGA 1 cut(s) 737
TasI AATT 7 cut(s) 10, 88, 485, 605, 619, 625, 903
TatI WGTACW 1 cut(s) 765
TfiI GAWTC 2 cut(s) 826, 1280
Tru1I TTAA 6 cut(s) 108, 930, 1170, 1223, 1235, 1317
Tru9I TTAA 6 cut(s) 108, 930, 1170, 1223, 1235, 1317
TscAI CASTG 5 cut(s) 307, 321, 459, 1093, 1149
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 3 cut(s) 72, 706, 877
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 6 cut(s) 618, 702, 912, 914, 1175, 1377
TspGWI ACGGA 1 cut(s) 420
TspRI CASTG 5 cut(s) 307, 321, 459, 1093, 1149
XapI RAATTY 1 cut(s) 903
XceI RCATGY 1 cut(s) 857
XspI CTAG 6 cut(s) 408, 480, 894, 989, 1079, 1341
Zsp2I ATGCAT 3 cut(s) 334, 952, 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.