Belongs to the protein kinase superfamily. Ser Thr protein kinase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 501, 861 |
| AccII | CGCG | 1 cut(s) | 375 |
| AciI | CCGC | 4 cut(s) | 27, 347, 375, 430 |
| AcoI | YGGCCR | 1 cut(s) | 1116 |
| AcsI | RAATTY | 2 cut(s) | 126, 763 |
| AfaI | GTAC | 6 cut(s) | 161, 199, 357, 447, 546, 827 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 178, 442, 827, 883 |
| AflIII | ACRYGT | 1 cut(s) | 918 |
| AjnI | CCWGG | 2 cut(s) | 162, 924 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 829, 861 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 905, 937 |
| AluBI | AGCT | 9 cut(s) | 103, 254, 297, 380, 398, 525, 783, 962, 1056 |
| AluI | AGCT | 9 cut(s) | 103, 254, 297, 380, 398, 525, 783, 962, 1056 |
| Alw21I | GWGCWC | 2 cut(s) | 324, 675 |
| Alw26I | GTCTC | 1 cut(s) | 329 |
| Alw44I | GTGCAC | 1 cut(s) | 671 |
| Ama87I | CYCGRG | 1 cut(s) | 35 |
| AoxI | GGCC | 3 cut(s) | 408, 630, 1116 |
| ApaLI | GTGCAC | 1 cut(s) | 671 |
| ApeKI | GCWGC | 4 cut(s) | 190, 294, 395, 538 |
| ApoI | RAATTY | 2 cut(s) | 126, 763 |
| AspA2I | CCTAGG | 1 cut(s) | 968 |
| AspS9I | GGNCC | 1 cut(s) | 409 |
| AsuHPI | GGTGA | 4 cut(s) | 130, 344, 491, 743 |
| AvaI | CYCGRG | 1 cut(s) | 35 |
| AvrII | CCTAGG | 1 cut(s) | 968 |
| BaeGI | GKGCMC | 1 cut(s) | 675 |
| BalI | TGGCCA | 1 cut(s) | 1118 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 738, 770 |
| Bbv12I | GWGCWC | 2 cut(s) | 324, 675 |
| BbvI | GCAGC | 4 cut(s) | 202, 306, 407, 525 |
| BccI | CCATC | 2 cut(s) | 586, 1108 |
| BceAI | ACGGC | 1 cut(s) | 92 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 571, 605 |
| BciT130I | CCWGG | 2 cut(s) | 164, 926 |
| BclI | TGATCA | 1 cut(s) | 991 |
| BcoDI | GTCTC | 1 cut(s) | 329 |
| BfaI | CTAG | 6 cut(s) | 116, 149, 704, 969, 1001, 1104 |
| BfmI | CTRYAG | 1 cut(s) | 1092 |
| BisI | GCNGC | 5 cut(s) | 28, 191, 295, 396, 539 |
| BlnI | CCTAGG | 1 cut(s) | 968 |
| BlsI | GCNGC | 5 cut(s) | 29, 192, 296, 397, 540 |
| BmcAI | AGTACT | 2 cut(s) | 199, 357 |
| Bme1390I | CCNGG | 2 cut(s) | 164, 926 |
| BmeT110I | CYCGRG | 1 cut(s) | 35 |
| BmgT120I | GGNCC | 1 cut(s) | 409 |
| BmrFI | CCNGG | 2 cut(s) | 164, 926 |
| BmrI | ACTGGG | 1 cut(s) | 1045 |
| BmsI | GCATC | 2 cut(s) | 882, 1117 |
| BmuI | ACTGGG | 1 cut(s) | 1045 |
| BpmI | CTGGAG | 1 cut(s) | 804 |
| BpuEI | CTTGAG | 1 cut(s) | 668 |
| BsaJI | CCNNGG | 4 cut(s) | 163, 328, 633, 968 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 972, 1002 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 178, 442, 827, 883 |
| Bse1I | ACTGG | 1 cut(s) | 1040 |
| Bse3DI | GCAATG | 2 cut(s) | 609, 700 |
| BseBI | CCWGG | 2 cut(s) | 164, 926 |
| BseDI | CCNNGG | 4 cut(s) | 163, 328, 633, 968 |
| BseGI | GGATG | 3 cut(s) | 67, 873, 1119 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 178, 442, 827, 883 |
| BseMI | GCAATG | 2 cut(s) | 609, 700 |
| BseMII | CTCAG | 5 cut(s) | 77, 390, 563, 588, 1128 |
| BseNI | ACTGG | 1 cut(s) | 1040 |
| BseRI | GAGGAG | 1 cut(s) | 807 |
| BseSI | GKGCMC | 1 cut(s) | 675 |
| BseXI | GCAGC | 4 cut(s) | 202, 306, 407, 525 |
| BsgI | GTGCAG | 1 cut(s) | 309 |
| Bsh1236I | CGCG | 1 cut(s) | 375 |
| BshFI | GGCC | 3 cut(s) | 410, 632, 1118 |
| BsiHKAI | GWGCWC | 2 cut(s) | 324, 675 |
| BsiHKCI | CYCGRG | 1 cut(s) | 35 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 178, 442, 827, 883 |
| BsmAI | GTCTC | 1 cut(s) | 329 |
| BsnI | GGCC | 3 cut(s) | 410, 632, 1118 |
| BsoBI | CYCGRG | 1 cut(s) | 35 |
| Bsp1286I | GDGCHC | 2 cut(s) | 324, 675 |
| Bsp143I | GATC | 1 cut(s) | 991 |
| BspACI | CCGC | 4 cut(s) | 27, 347, 375, 430 |
| BspANI | GGCC | 3 cut(s) | 410, 632, 1118 |
| BspCNI | CTCAG | 5 cut(s) | 78, 391, 562, 589, 1129 |
| BspFNI | CGCG | 1 cut(s) | 375 |
| BspMAI | CTGCAG | 1 cut(s) | 1096 |
| BsrDI | GCAATG | 2 cut(s) | 609, 700 |
| BsrI | ACTGG | 1 cut(s) | 1040 |
| BssECI | CCNNGG | 4 cut(s) | 163, 328, 633, 968 |
| BssMI | GATC | 1 cut(s) | 991 |
| BssNAI | GTATAC | 2 cut(s) | 502, 862 |
| BssT1I | CCWWGG | 3 cut(s) | 328, 633, 968 |
| Bst1107I | GTATAC | 2 cut(s) | 502, 862 |
| Bst2UI | CCWGG | 2 cut(s) | 164, 926 |
| Bst4CI | ACNGT | 4 cut(s) | 450, 677, 830, 866 |
| BstC8I | GCNNGC | 2 cut(s) | 292, 1054 |
| BstDEI | CTNAG | 7 cut(s) | 86, 399, 422, 526, 549, 597, 1137 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 176, 440 |
| BstF5I | GGATG | 3 cut(s) | 67, 873, 1119 |
| BstFNI | CGCG | 1 cut(s) | 375 |
| BstKTI | GATC | 1 cut(s) | 994 |
| BstMAI | GTCTC | 1 cut(s) | 329 |
| BstMBI | GATC | 1 cut(s) | 991 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 670, 1091 |
| BstNI | CCWGG | 2 cut(s) | 164, 926 |
| BstNSI | RCATGY | 4 cut(s) | 459, 545, 728, 922 |
| BstSCI | CCNGG | 2 cut(s) | 162, 924 |
| BstSFI | CTRYAG | 1 cut(s) | 1092 |
| BstSLI | GKGCMC | 1 cut(s) | 675 |
| BstUI | CGCG | 1 cut(s) | 375 |
| BstV1I | GCAGC | 4 cut(s) | 202, 306, 407, 525 |
| BstZ17I | GTATAC | 2 cut(s) | 502, 862 |
| BsuRI | GGCC | 3 cut(s) | 410, 632, 1118 |
| BtgZI | GCGATG | 2 cut(s) | 356, 995 |
| BtsCI | GGATG | 3 cut(s) | 67, 873, 1119 |
| BtsIMutI | CAGTG | 1 cut(s) | 673 |
| Cac8I | GCNNGC | 2 cut(s) | 292, 1054 |
| Cfr13I | GGNCC | 1 cut(s) | 409 |
| Csp6I | GTAC | 6 cut(s) | 160, 198, 356, 446, 545, 826 |
| CviAII | CATG | 8 cut(s) | 54, 456, 511, 542, 725, 919, 995, 1166 |
| CviQI | GTAC | 6 cut(s) | 160, 198, 356, 446, 545, 826 |
| DdeI | CTNAG | 7 cut(s) | 86, 399, 422, 526, 549, 597, 1137 |
| DpnI | GATC | 1 cut(s) | 993 |
| DpnII | GATC | 1 cut(s) | 991 |
| EaeI | YGGCCR | 1 cut(s) | 1116 |
| Eco130I | CCWWGG | 3 cut(s) | 328, 633, 968 |
| Eco88I | CYCGRG | 1 cut(s) | 35 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 176, 440 |
| EcoO109I | RGGNCCY | 1 cut(s) | 409 |
| EcoRI | GAATTC | 1 cut(s) | 126 |
| EcoRII | CCWGG | 2 cut(s) | 162, 924 |
| EcoT14I | CCWWGG | 3 cut(s) | 328, 633, 968 |
| ErhI | CCWWGG | 3 cut(s) | 328, 633, 968 |
| FaeI | CATG | 8 cut(s) | 57, 459, 514, 545, 728, 922, 998, 1169 |
| FatI | CATG | 8 cut(s) | 53, 455, 510, 541, 724, 918, 994, 1165 |
| FbaI | TGATCA | 1 cut(s) | 991 |
| FblI | GTMKAC | 2 cut(s) | 501, 861 |
| Fnu4HI | GCNGC | 5 cut(s) | 28, 191, 295, 396, 539 |
| FokI | GGATG | 3 cut(s) | 54, 860, 1126 |
| Fsp4HI | GCNGC | 5 cut(s) | 28, 191, 295, 396, 539 |
| FspBI | CTAG | 6 cut(s) | 116, 149, 704, 969, 1001, 1104 |
| GluI | GCNGC | 5 cut(s) | 28, 191, 295, 396, 539 |
| GsuI | CTGGAG | 1 cut(s) | 804 |
| HaeIII | GGCC | 3 cut(s) | 410, 632, 1118 |
| Hin1II | CATG | 8 cut(s) | 57, 459, 514, 545, 728, 922, 998, 1169 |
| HincII | GTYRAC | 1 cut(s) | 517 |
| HindII | GTYRAC | 1 cut(s) | 517 |
| HindIII | AAGCTT | 2 cut(s) | 523, 960 |
| HinfI | GANTC | 3 cut(s) | 135, 209, 750 |
| HpaI | GTTAAC | 1 cut(s) | 517 |
| HphI | GGTGA | 4 cut(s) | 130, 344, 491, 743 |
| Hpy166II | GTNNAC | 5 cut(s) | 502, 517, 673, 862, 894 |
| Hpy188I | TCNGA | 4 cut(s) | 17, 552, 598, 1149 |
| Hpy188III | TCNNGA | 3 cut(s) | 704, 768, 821 |
| Hpy8I | GTNNAC | 5 cut(s) | 502, 517, 673, 862, 894 |
| Hpy99I | CGWCG | 1 cut(s) | 913 |
| HpyAV | CCTTC | 5 cut(s) | 106, 172, 436, 764, 950 |
| HpyCH4III | ACNGT | 4 cut(s) | 450, 677, 830, 866 |
| HpyCH4IV | ACGT | 1 cut(s) | 621 |
| HpyCH4V | TGCA | 8 cut(s) | 57, 290, 395, 455, 541, 614, 673, 1094 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 670, 1091 |
| HpyF3I | CTNAG | 7 cut(s) | 86, 399, 422, 526, 549, 597, 1137 |
| HpySE526I | ACGT | 1 cut(s) | 621 |
| Hsp92II | CATG | 8 cut(s) | 57, 459, 514, 545, 728, 922, 998, 1169 |
| Ksp22I | TGATCA | 1 cut(s) | 991 |
| KspAI | GTTAAC | 1 cut(s) | 517 |
| Kzo9I | GATC | 1 cut(s) | 991 |
| LmnI | GCTCC | 2 cut(s) | 377, 820 |
| Lsp1109I | GCAGC | 4 cut(s) | 202, 306, 407, 525 |
| LweI | GCATC | 2 cut(s) | 882, 1117 |
| MaeI | CTAG | 6 cut(s) | 116, 149, 704, 969, 1001, 1104 |
| MaeII | ACGT | 1 cut(s) | 621 |
| MaeIII | GTNAC | 1 cut(s) | 677 |
| MalI | GATC | 1 cut(s) | 993 |
| MboI | GATC | 1 cut(s) | 991 |
| MboII | GAAGA | 2 cut(s) | 261, 692 |
| MfeI | CAATTG | 3 cut(s) | 342, 615, 804 |
| MhlI | GDGCHC | 2 cut(s) | 324, 675 |
| MlsI | TGGCCA | 1 cut(s) | 1118 |
| MluNI | TGGCCA | 1 cut(s) | 1118 |
| MmeI | TCCRAC | 2 cut(s) | 864, 1127 |
| MnlI | CCTC | 7 cut(s) | 74, 400, 523, 558, 594, 692, 828 |
| Mox20I | TGGCCA | 1 cut(s) | 1118 |
| MscI | TGGCCA | 1 cut(s) | 1118 |
| MseI | TTAA | 5 cut(s) | 249, 281, 516, 587, 795 |
| MslI | CAYNNNNRTG | 1 cut(s) | 729 |
| Msp20I | TGGCCA | 1 cut(s) | 1118 |
| MspA1I | CMGCKG | 1 cut(s) | 398 |
| MspR9I | CCNGG | 2 cut(s) | 164, 926 |
| MunI | CAATTG | 3 cut(s) | 342, 615, 804 |
| MvaI | CCWGG | 2 cut(s) | 164, 926 |
| MvnI | CGCG | 1 cut(s) | 375 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 670, 1091 |
| NdeII | GATC | 1 cut(s) | 991 |
| NlaIII | CATG | 8 cut(s) | 57, 459, 514, 545, 728, 922, 998, 1169 |
| NmeAIII | GCCGAG | 1 cut(s) | 714 |
| NmuCI | GTSAC | 1 cut(s) | 677 |
| NspI | RCATGY | 4 cut(s) | 459, 545, 728, 922 |
| PciI | ACATGT | 1 cut(s) | 918 |
| PfeI | GAWTC | 3 cut(s) | 135, 209, 750 |
| PflFI | GACNNNGTC | 1 cut(s) | 1037 |
| PfoI | TCCNGGA | 1 cut(s) | 924 |
| PkrI | GCNGC | 5 cut(s) | 29, 192, 296, 397, 540 |
| PscI | ACATGT | 1 cut(s) | 918 |
| Psp6I | CCWGG | 2 cut(s) | 162, 924 |
| PspGI | CCWGG | 2 cut(s) | 162, 924 |
| PspPI | GGNCC | 1 cut(s) | 409 |
| PstI | CTGCAG | 1 cut(s) | 1096 |
| PsyI | GACNNNGTC | 1 cut(s) | 1037 |
| PvuII | CAGCTG | 1 cut(s) | 398 |
| RsaI | GTAC | 6 cut(s) | 161, 199, 357, 447, 546, 827 |
| RsaNI | GTAC | 6 cut(s) | 160, 198, 356, 446, 545, 826 |
| RseI | CAYNNNNRTG | 1 cut(s) | 729 |
| SaqAI | TTAA | 5 cut(s) | 249, 281, 516, 587, 795 |
| SatI | GCNGC | 5 cut(s) | 28, 191, 295, 396, 539 |
| Sau3AI | GATC | 1 cut(s) | 991 |
| Sau96I | GGNCC | 1 cut(s) | 409 |
| ScaI | AGTACT | 2 cut(s) | 199, 357 |
| ScrFI | CCNGG | 2 cut(s) | 164, 926 |
| SduI | GDGCHC | 2 cut(s) | 324, 675 |
| SfaNI | GCATC | 2 cut(s) | 882, 1117 |
| SfcI | CTRYAG | 1 cut(s) | 1092 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 729 |
| SmlI | CTYRAG | 1 cut(s) | 683 |
| SmoI | CTYRAG | 1 cut(s) | 683 |
| SsiI | CCGC | 4 cut(s) | 27, 347, 375, 430 |
| SspMI | CTAG | 6 cut(s) | 116, 149, 704, 969, 1001, 1104 |
| StyD4I | CCNGG | 2 cut(s) | 162, 924 |
| StyI | CCWWGG | 3 cut(s) | 328, 633, 968 |
| TaaI | ACNGT | 4 cut(s) | 450, 677, 830, 866 |
| TaiI | ACGT | 1 cut(s) | 624 |
| TaqI | TCGA | 2 cut(s) | 324, 581 |
| TaqII | GACCGA | 1 cut(s) | 922 |
| TatI | WGTACW | 2 cut(s) | 197, 355 |
| TauI | GCSGC | 1 cut(s) | 30 |
| TfiI | GAWTC | 3 cut(s) | 135, 209, 750 |
| Tru1I | TTAA | 5 cut(s) | 249, 281, 516, 587, 795 |
| Tru9I | TTAA | 5 cut(s) | 249, 281, 516, 587, 795 |
| TscAI | CASTG | 1 cut(s) | 680 |
| TseFI | GTSAC | 1 cut(s) | 677 |
| TseI | GCWGC | 4 cut(s) | 190, 294, 395, 538 |
| Tsp45I | GTSAC | 1 cut(s) | 677 |
| TspDTI | ATGAA | 7 cut(s) | 17, 359, 499, 585, 855, 932, 1154 |
| TspGWI | ACGGA | 4 cut(s) | 190, 534, 926, 1126 |
| TspRI | CASTG | 1 cut(s) | 680 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1037 |
| VneI | GTGCAC | 1 cut(s) | 671 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 176, 440 |
| XapI | RAATTY | 2 cut(s) | 126, 763 |
| XbaI | TCTAGA | 1 cut(s) | 703 |
| XceI | RCATGY | 4 cut(s) | 459, 545, 728, 922 |
| XmaJI | CCTAGG | 1 cut(s) | 968 |
| XmiI | GTMKAC | 2 cut(s) | 501, 861 |
| XspI | CTAG | 6 cut(s) | 116, 149, 704, 969, 1001, 1104 |
| ZrmI | AGTACT | 2 cut(s) | 199, 357 |