Belongs to the protein kinase superfamily. Ser Thr protein kinase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 380 |
| Acc36I | ACCTGC | 2 cut(s) | 380, 535 |
| Acc65I | GGTACC | 1 cut(s) | 1151 |
| AccB1I | GGYRCC | 4 cut(s) | 578, 1090, 1151, 3038 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 2561, 2849 |
| AccI | GTMKAC | 2 cut(s) | 379, 2886 |
| AciI | CCGC | 7 cut(s) | 88, 95, 120, 201, 1123, 1616, 2037 |
| AclWI | GGATC | 7 cut(s) | 15, 95, 302, 429, 442, 721, 2830 |
| AcoI | YGGCCR | 4 cut(s) | 44, 174, 846, 2406 |
| AcsI | RAATTY | 6 cut(s) | 654, 930, 1486, 1714, 2443, 2592 |
| AcuI | CTGAAG | 4 cut(s) | 1946, 1995, 2265, 3075 |
| AcyI | GRCGYC | 1 cut(s) | 366 |
| AfaI | GTAC | 4 cut(s) | 1153, 1937, 1974, 2321 |
| AflII | CTTAAG | 1 cut(s) | 469 |
| AflIII | ACRYGT | 3 cut(s) | 2373, 2734, 2943 |
| AgeI | ACCGGT | 1 cut(s) | 1154 |
| AhlI | ACTAGT | 3 cut(s) | 64, 2780, 3022 |
| AjnI | CCWGG | 1 cut(s) | 1224 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1913, 1945 |
| Alw21I | GWGCWC | 4 cut(s) | 224, 295, 1726, 3091 |
| Alw26I | GTCTC | 7 cut(s) | 238, 254, 759, 1036, 1186, 2106, 2663 |
| Alw44I | GTGCAC | 1 cut(s) | 1722 |
| AlwI | GGATC | 7 cut(s) | 15, 95, 302, 429, 442, 721, 2830 |
| Ama87I | CYCGRG | 2 cut(s) | 145, 485 |
| AoxI | GGCC | 6 cut(s) | 44, 174, 846, 1014, 1630, 2406 |
| ApaLI | GTGCAC | 1 cut(s) | 1722 |
| ApeKI | GCWGC | 6 cut(s) | 9, 111, 182, 1392, 2390, 2837 |
| ApoI | RAATTY | 6 cut(s) | 654, 930, 1486, 1714, 2443, 2592 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1160, 1192 |
| AsiGI | ACCGGT | 1 cut(s) | 1154 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 342, 1581, 2332 |
| Asp718I | GGTACC | 1 cut(s) | 1151 |
| AspA2I | CCTAGG | 1 cut(s) | 1552 |
| AspLEI | GCGC | 1 cut(s) | 3210 |
| AspS9I | GGNCC | 2 cut(s) | 1014, 1826 |
| AsuC2I | CCSGG | 2 cut(s) | 317, 2137 |
| AsuHPI | GGTGA | 6 cut(s) | 361, 409, 607, 904, 1178, 2732 |
| AsuNHI | GCTAGC | 1 cut(s) | 280 |
| AvaI | CYCGRG | 2 cut(s) | 145, 485 |
| AvaII | GGWCC | 1 cut(s) | 1826 |
| AvrII | CCTAGG | 1 cut(s) | 1552 |
| BaeGI | GKGCMC | 1 cut(s) | 1726 |
| BalI | TGGCCA | 1 cut(s) | 848 |
| BamHI | GGATCC | 1 cut(s) | 434 |
| BanI | GGYRCC | 4 cut(s) | 578, 1090, 1151, 3038 |
| BanII | GRGCYC | 3 cut(s) | 224, 295, 419 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 859, 891, 1528, 1560, 1919, 1951 |
| BbsI | GAAGAC | 3 cut(s) | 25, 935, 2987 |
| Bbv12I | GWGCWC | 4 cut(s) | 224, 295, 1726, 3091 |
| BbvCI | CCTCAGC | 1 cut(s) | 1772 |
| BbvI | GCAGC | 6 cut(s) | 21, 123, 169, 1404, 2402, 2824 |
| BceAI | ACGGC | 2 cut(s) | 133, 379 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 208, 242 |
| BciT130I | CCWGG | 1 cut(s) | 1226 |
| BciVI | GTATCC | 1 cut(s) | 2878 |
| BclI | TGATCA | 1 cut(s) | 1191 |
| BcnI | CCSGG | 2 cut(s) | 317, 2137 |
| BcoDI | GTCTC | 7 cut(s) | 238, 254, 759, 1036, 1186, 2106, 2663 |
| BcuI | ACTAGT | 3 cut(s) | 64, 2780, 3022 |
| BfmI | CTRYAG | 2 cut(s) | 373, 2887 |
| BfrI | CTTAAG | 1 cut(s) | 469 |
| BfuAI | ACCTGC | 2 cut(s) | 380, 535 |
| BfuI | GTATCC | 1 cut(s) | 2878 |
| BglII | AGATCT | 1 cut(s) | 2454 |
| BisI | GCNGC | 9 cut(s) | 10, 89, 96, 112, 121, 183, 1393, 2391, 2838 |
| BlnI | CCTAGG | 1 cut(s) | 1552 |
| BlsI | GCNGC | 9 cut(s) | 11, 90, 97, 113, 122, 184, 1394, 2392, 2839 |
| Bme1390I | CCNGG | 3 cut(s) | 317, 1226, 2137 |
| Bme18I | GGWCC | 1 cut(s) | 1826 |
| BmeT110I | CYCGRG | 2 cut(s) | 145, 485 |
| BmgT120I | GGNCC | 2 cut(s) | 1014, 1826 |
| BmrFI | CCNGG | 3 cut(s) | 317, 1226, 2137 |
| BmrI | ACTGGG | 2 cut(s) | 1020, 1758 |
| BmsI | GCATC | 8 cut(s) | 753, 807, 952, 1332, 1404, 1548, 2560, 3194 |
| BmtI | GCTAGC | 1 cut(s) | 284 |
| BmuI | ACTGGG | 2 cut(s) | 1020, 1758 |
| BpiI | GAAGAC | 3 cut(s) | 25, 935, 2987 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 401, 433 |
| BpmI | CTGGAG | 5 cut(s) | 138, 428, 1773, 2001, 2589 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1772, 2994 |
| BpuEI | CTTGAG | 5 cut(s) | 405, 743, 976, 1937, 2678 |
| BpuMI | CCSGG | 2 cut(s) | 317, 2137 |
| Bsa29I | ATCGAT | 1 cut(s) | 2825 |
| BsaBI | GATNNNNATC | 2 cut(s) | 439, 1143 |
| BsaHI | GRCGYC | 1 cut(s) | 366 |
| BsaJI | CCNNGG | 5 cut(s) | 177, 250, 1035, 1225, 1552 |
| BsaWI | WCCGGW | 1 cut(s) | 1154 |
| Bse118I | RCCGGY | 1 cut(s) | 1154 |
| Bse3DI | GCAATG | 5 cut(s) | 275, 990, 1214, 2497, 2619 |
| Bse8I | GATNNNNATC | 2 cut(s) | 439, 1143 |
| BseBI | CCWGG | 1 cut(s) | 1226 |
| BseCI | ATCGAT | 1 cut(s) | 2825 |
| BseDI | CCNNGG | 5 cut(s) | 177, 250, 1035, 1225, 1552 |
| BseJI | GATNNNNATC | 2 cut(s) | 439, 1143 |
| BseMI | GCAATG | 5 cut(s) | 275, 990, 1214, 2497, 2619 |
| BseMII | CTCAG | 7 cut(s) | 308, 1041, 1197, 1786, 2272, 2781, 2985 |
| BseRI | GAGGAG | 2 cut(s) | 507, 2246 |
| BseSI | GKGCMC | 1 cut(s) | 1726 |
| BseXI | GCAGC | 6 cut(s) | 21, 123, 169, 1404, 2402, 2824 |
| BseYI | CCCAGC | 1 cut(s) | 412 |
| BsgI | GTGCAG | 1 cut(s) | 2823 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2679 |
| BshFI | GGCC | 6 cut(s) | 46, 176, 848, 1016, 1632, 2408 |
| BshNI | GGYRCC | 4 cut(s) | 578, 1090, 1151, 3038 |
| BshTI | ACCGGT | 1 cut(s) | 1154 |
| BshVI | ATCGAT | 1 cut(s) | 2825 |
| BsiEI | CGRYCG | 1 cut(s) | 2679 |
| BsiHKAI | GWGCWC | 4 cut(s) | 224, 295, 1726, 3091 |
| BsiHKCI | CYCGRG | 2 cut(s) | 145, 485 |
| BsiSI | CCGG | 5 cut(s) | 43, 173, 316, 1155, 2136 |
| BslFI | GGGAC | 3 cut(s) | 1258, 1839, 2166 |
| BsmAI | GTCTC | 7 cut(s) | 238, 254, 759, 1036, 1186, 2106, 2663 |
| BsmBI | CGTCTC | 2 cut(s) | 254, 759 |
| BsmFI | GGGAC | 3 cut(s) | 1258, 1839, 2166 |
| BsmI | GAATGC | 3 cut(s) | 1467, 2002, 2898 |
| BsnI | GGCC | 6 cut(s) | 46, 176, 848, 1016, 1632, 2408 |
| BsoBI | CYCGRG | 2 cut(s) | 145, 485 |
| Bsp1286I | GDGCHC | 5 cut(s) | 224, 295, 419, 1726, 3091 |
| Bsp1407I | TGTACA | 1 cut(s) | 2319 |
| BspACI | CCGC | 7 cut(s) | 88, 95, 120, 201, 1123, 1616, 2037 |
| BspANI | GGCC | 6 cut(s) | 46, 176, 848, 1016, 1632, 2408 |
| BspCNI | CTCAG | 7 cut(s) | 307, 1040, 1196, 1785, 2271, 2780, 2986 |
| BspDI | ATCGAT | 1 cut(s) | 2825 |
| BspMAI | CTGCAG | 1 cut(s) | 377 |
| BspMI | ACCTGC | 2 cut(s) | 380, 535 |
| BspOI | GCTAGC | 1 cut(s) | 284 |
| BspPI | GGATC | 7 cut(s) | 15, 95, 302, 429, 442, 721, 2830 |
| BspT107I | GGYRCC | 4 cut(s) | 578, 1090, 1151, 3038 |
| BspTI | CTTAAG | 1 cut(s) | 469 |
| BsrDI | GCAATG | 5 cut(s) | 275, 990, 1214, 2497, 2619 |
| BsrFI | RCCGGY | 1 cut(s) | 1154 |
| BsrGI | TGTACA | 1 cut(s) | 2319 |
| BssAI | RCCGGY | 1 cut(s) | 1154 |
| BssECI | CCNNGG | 5 cut(s) | 177, 250, 1035, 1225, 1552 |
| BssNI | GRCGYC | 1 cut(s) | 366 |
| BssT1I | CCWWGG | 4 cut(s) | 177, 250, 1035, 1552 |
| Bst2UI | CCWGG | 1 cut(s) | 1226 |
| Bst4CI | ACNGT | 3 cut(s) | 916, 2957, 3071 |
| Bst6I | CTCTTC | 2 cut(s) | 2535, 3096 |
| BstACI | GRCGYC | 1 cut(s) | 366 |
| BstAFI | CTTAAG | 1 cut(s) | 469 |
| BstAUI | TGTACA | 1 cut(s) | 2319 |
| BstC8I | GCNNGC | 7 cut(s) | 93, 187, 220, 273, 282, 768, 2072 |
| BstEII | GGTNACC | 1 cut(s) | 2720 |
| BstHHI | GCGC | 1 cut(s) | 3210 |
| BstMAI | GTCTC | 7 cut(s) | 238, 254, 759, 1036, 1186, 2106, 2663 |
| BstMCI | CGRYCG | 1 cut(s) | 2679 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 117, 120, 182, 277, 281, 290, 372, 541 |
| BstNI | CCWGG | 1 cut(s) | 1226 |
| BstNSI | RCATGY | 5 cut(s) | 2377, 2469, 2738, 2947, 3141 |
| BstPI | GGTNACC | 1 cut(s) | 2720 |
| BstSCI | CCNGG | 3 cut(s) | 315, 1224, 2135 |
| BstSFI | CTRYAG | 2 cut(s) | 373, 2887 |
| BstSLI | GKGCMC | 1 cut(s) | 1726 |
| BstV1I | GCAGC | 6 cut(s) | 21, 123, 169, 1404, 2402, 2824 |
| BstV2I | GAAGAC | 3 cut(s) | 25, 935, 2987 |
| BstX2I | RGATCY | 4 cut(s) | 100, 434, 726, 2454 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 1292, 1316, 1628, 1679 |
| BstYI | RGATCY | 4 cut(s) | 100, 434, 726, 2454 |
| Bsu15I | ATCGAT | 1 cut(s) | 2825 |
| BsuI | GTATCC | 1 cut(s) | 2878 |
| BsuRI | GGCC | 6 cut(s) | 46, 176, 848, 1016, 1632, 2408 |
| BsuTUI | ATCGAT | 1 cut(s) | 2825 |
| BtgZI | GCGATG | 4 cut(s) | 33, 43, 371, 975 |
| BtsIMutI | CAGTG | 6 cut(s) | 362, 1135, 1677, 1724, 2934, 2956 |
| BveI | ACCTGC | 2 cut(s) | 380, 535 |
| Cac8I | GCNNGC | 7 cut(s) | 93, 187, 220, 273, 282, 768, 2072 |
| CfoI | GCGC | 1 cut(s) | 3210 |
| Cfr10I | RCCGGY | 1 cut(s) | 1154 |
| Cfr13I | GGNCC | 2 cut(s) | 1014, 1826 |
| ClaI | ATCGAT | 1 cut(s) | 2825 |
| CseI | GACGC | 1 cut(s) | 355 |
| Csp6I | GTAC | 4 cut(s) | 1152, 1936, 1973, 2320 |
| CspAI | ACCGGT | 1 cut(s) | 1154 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 178, 213, 1032, 1067, 1390, 1425 |
| CviQI | GTAC | 4 cut(s) | 1152, 1936, 1973, 2320 |
| EaeI | YGGCCR | 4 cut(s) | 44, 174, 846, 2406 |
| Eam1104I | CTCTTC | 2 cut(s) | 2535, 3096 |
| EarI | CTCTTC | 2 cut(s) | 2535, 3096 |
| Ecl136II | GAGCTC | 2 cut(s) | 222, 293 |
| Eco130I | CCWWGG | 4 cut(s) | 177, 250, 1035, 1552 |
| Eco24I | GRGCYC | 3 cut(s) | 224, 295, 419 |
| Eco47I | GGWCC | 1 cut(s) | 1826 |
| Eco53kI | GAGCTC | 2 cut(s) | 222, 293 |
| Eco57I | CTGAAG | 4 cut(s) | 1946, 1995, 2265, 3075 |
| Eco88I | CYCGRG | 2 cut(s) | 145, 485 |
| Eco91I | GGTNACC | 1 cut(s) | 2720 |
| EcoICRI | GAGCTC | 2 cut(s) | 222, 293 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1826 |
| EcoO65I | GGTNACC | 1 cut(s) | 2720 |
| EcoRII | CCWGG | 1 cut(s) | 1224 |
| EcoT14I | CCWWGG | 4 cut(s) | 177, 250, 1035, 1552 |
| EcoT38I | GRGCYC | 3 cut(s) | 224, 295, 419 |
| ErhI | CCWWGG | 4 cut(s) | 177, 250, 1035, 1552 |
| Esp3I | CGTCTC | 2 cut(s) | 254, 759 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 927, 959, 1993, 2025, 2316, 2348 |
| FaqI | GGGAC | 3 cut(s) | 1258, 1839, 2166 |
| FauNDI | CATATG | 1 cut(s) | 2245 |
| FbaI | TGATCA | 1 cut(s) | 1191 |
| FblI | GTMKAC | 2 cut(s) | 379, 2886 |
| Fnu4HI | GCNGC | 9 cut(s) | 10, 89, 96, 112, 121, 183, 1393, 2391, 2838 |
| FriOI | GRGCYC | 3 cut(s) | 224, 295, 419 |
| Fsp4HI | GCNGC | 9 cut(s) | 10, 89, 96, 112, 121, 183, 1393, 2391, 2838 |
| GlaI | GCGC | 1 cut(s) | 3209 |
| GluI | GCNGC | 9 cut(s) | 10, 89, 96, 112, 121, 183, 1393, 2391, 2838 |
| GsaI | CCCAGC | 1 cut(s) | 416 |
| GsuI | CTGGAG | 5 cut(s) | 138, 428, 1773, 2001, 2589 |
| HaeIII | GGCC | 6 cut(s) | 46, 176, 848, 1016, 1632, 2408 |
| HapII | CCGG | 5 cut(s) | 43, 173, 316, 1155, 2136 |
| HgaI | GACGC | 1 cut(s) | 355 |
| HhaI | GCGC | 1 cut(s) | 3210 |
| Hin1I | GRCGYC | 1 cut(s) | 366 |
| Hin6I | GCGC | 1 cut(s) | 3208 |
| HinP1I | GCGC | 1 cut(s) | 3208 |
| HincII | GTYRAC | 2 cut(s) | 380, 1500 |
| HindII | GTYRAC | 2 cut(s) | 380, 1500 |
| HindIII | AAGCTT | 5 cut(s) | 423, 463, 683, 2072, 2803 |
| HpaI | GTTAAC | 1 cut(s) | 1500 |
| HpaII | CCGG | 5 cut(s) | 43, 173, 316, 1155, 2136 |
| HphI | GGTGA | 6 cut(s) | 361, 409, 607, 904, 1178, 2732 |
| Hpy166II | GTNNAC | 8 cut(s) | 380, 1500, 1724, 2142, 2320, 2512, 2887, 2919 |
| Hpy8I | GTNNAC | 8 cut(s) | 380, 1500, 1724, 2142, 2320, 2512, 2887, 2919 |
| Hpy99I | CGWCG | 3 cut(s) | 17, 119, 384 |
| HpyCH4III | ACNGT | 3 cut(s) | 916, 2957, 3071 |
| HpyCH4IV | ACGT | 2 cut(s) | 1247, 1731 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 117, 120, 182, 277, 281, 290, 372, 541 |
| HpySE526I | ACGT | 2 cut(s) | 1247, 1731 |
| Hsp92I | GRCGYC | 1 cut(s) | 366 |
| HspAI | GCGC | 1 cut(s) | 3208 |
| KflI | GGGWCCC | 1 cut(s) | 1826 |
| KpnI | GGTACC | 1 cut(s) | 1155 |
| Ksp22I | TGATCA | 1 cut(s) | 1191 |
| KspAI | GTTAAC | 1 cut(s) | 1500 |
| LmnI | GCTCC | 7 cut(s) | 227, 409, 1385, 1464, 1967, 2387, 2532 |
| Lsp1109I | GCAGC | 6 cut(s) | 21, 123, 169, 1404, 2402, 2824 |
| LweI | GCATC | 8 cut(s) | 753, 807, 952, 1332, 1404, 1548, 2560, 3194 |
| MaeII | ACGT | 2 cut(s) | 1247, 1731 |
| MfeI | CAATTG | 4 cut(s) | 230, 601, 818, 1511 |
| MflI | RGATCY | 4 cut(s) | 100, 434, 726, 2454 |
| MhlI | GDGCHC | 5 cut(s) | 224, 295, 419, 1726, 3091 |
| MlsI | TGGCCA | 1 cut(s) | 848 |
| MluNI | TGGCCA | 1 cut(s) | 848 |
| MlyI | GAGTC | 6 cut(s) | 240, 1500, 2025, 2075, 3026, 3106 |
| MmeI | TCCRAC | 5 cut(s) | 48, 128, 1279, 1911, 2939 |
| Mox20I | TGGCCA | 1 cut(s) | 848 |
| MroXI | GAANNNNTTC | 3 cut(s) | 342, 1581, 2332 |
| MscI | TGGCCA | 1 cut(s) | 848 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2903, 2973 |
| Msp20I | TGGCCA | 1 cut(s) | 848 |
| MspCI | CTTAAG | 1 cut(s) | 469 |
| MspI | CCGG | 5 cut(s) | 43, 173, 316, 1155, 2136 |
| MspR9I | CCNGG | 3 cut(s) | 317, 1226, 2137 |
| MunI | CAATTG | 4 cut(s) | 230, 601, 818, 1511 |
| Mva1269I | GAATGC | 3 cut(s) | 1467, 2002, 2898 |
| MvaI | CCWGG | 1 cut(s) | 1226 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 117, 120, 182, 277, 281, 290, 372, 541 |
| NciI | CCSGG | 2 cut(s) | 317, 2137 |
| NdeI | CATATG | 1 cut(s) | 2245 |
| NheI | GCTAGC | 1 cut(s) | 280 |
| NmeAIII | GCCGAG | 1 cut(s) | 64 |
| NmuCI | GTSAC | 7 cut(s) | 319, 367, 397, 1166, 2720, 2951, 3018 |
| NspI | RCATGY | 5 cut(s) | 2377, 2469, 2738, 2947, 3141 |
| PaeR7I | CTCGAG | 1 cut(s) | 485 |
| PaqCI | CACCTGC | 1 cut(s) | 380 |
| PciI | ACATGT | 3 cut(s) | 2373, 2734, 2943 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 21 |
| PctI | GAATGC | 3 cut(s) | 1467, 2002, 2898 |
| PdmI | GAANNNNTTC | 3 cut(s) | 342, 1581, 2332 |
| PfeI | GAWTC | 7 cut(s) | 141, 1342, 1861, 1921, 2328, 2962, 3149 |
| PflFI | GACNNNGTC | 1 cut(s) | 29 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 2561, 2849 |
| PinAI | ACCGGT | 1 cut(s) | 1154 |
| PkrI | GCNGC | 9 cut(s) | 11, 90, 97, 113, 122, 184, 1394, 2392, 2839 |
| Ple19I | CGATCG | 1 cut(s) | 2679 |
| PleI | GAGTC | 6 cut(s) | 240, 1500, 2025, 2075, 3025, 3106 |
| PpsI | GAGTC | 6 cut(s) | 240, 1500, 2025, 2075, 3025, 3106 |
| PpuMI | RGGWCCY | 1 cut(s) | 1826 |
| PscI | ACATGT | 3 cut(s) | 2373, 2734, 2943 |
| Psp124BI | GAGCTC | 2 cut(s) | 224, 295 |
| Psp5II | RGGWCCY | 1 cut(s) | 1826 |
| Psp6I | CCWGG | 1 cut(s) | 1224 |
| PspEI | GGTNACC | 1 cut(s) | 2720 |
| PspFI | CCCAGC | 1 cut(s) | 412 |
| PspGI | CCWGG | 1 cut(s) | 1224 |
| PspPI | GGNCC | 2 cut(s) | 1014, 1826 |
| PspPPI | RGGWCCY | 1 cut(s) | 1826 |
| PstI | CTGCAG | 1 cut(s) | 377 |
| PsuI | RGATCY | 4 cut(s) | 100, 434, 726, 2454 |
| PsyI | GACNNNGTC | 1 cut(s) | 29 |
| PvuI | CGATCG | 1 cut(s) | 2679 |
| RsaI | GTAC | 4 cut(s) | 1153, 1937, 1974, 2321 |
| RsaNI | GTAC | 4 cut(s) | 1152, 1936, 1973, 2320 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2903, 2973 |
| SacI | GAGCTC | 2 cut(s) | 224, 295 |
| SalI | GTCGAC | 1 cut(s) | 378 |
| SatI | GCNGC | 9 cut(s) | 10, 89, 96, 112, 121, 183, 1393, 2391, 2838 |
| Sau96I | GGNCC | 2 cut(s) | 1014, 1826 |
| SchI | GAGTC | 6 cut(s) | 240, 1500, 2025, 2075, 3026, 3106 |
| ScrFI | CCNGG | 3 cut(s) | 317, 1226, 2137 |
| SduI | GDGCHC | 5 cut(s) | 224, 295, 419, 1726, 3091 |
| SfaNI | GCATC | 8 cut(s) | 753, 807, 952, 1332, 1404, 1548, 2560, 3194 |
| SfcI | CTRYAG | 2 cut(s) | 373, 2887 |
| Sfr274I | CTCGAG | 1 cut(s) | 485 |
| SinI | GGWCC | 1 cut(s) | 1826 |
| SlaI | CTCGAG | 1 cut(s) | 485 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2903, 2973 |
| SmlI | CTYRAG | 7 cut(s) | 420, 469, 485, 758, 955, 1916, 2693 |
| SmoI | CTYRAG | 7 cut(s) | 420, 469, 485, 758, 955, 1916, 2693 |
| SpeI | ACTAGT | 3 cut(s) | 64, 2780, 3022 |
| SsiI | CCGC | 7 cut(s) | 88, 95, 120, 201, 1123, 1616, 2037 |
| SstI | GAGCTC | 2 cut(s) | 224, 295 |
| StyD4I | CCNGG | 3 cut(s) | 315, 1224, 2135 |
| StyI | CCWWGG | 4 cut(s) | 177, 250, 1035, 1552 |
| TaaI | ACNGT | 3 cut(s) | 916, 2957, 3071 |
| TaiI | ACGT | 2 cut(s) | 1250, 1734 |
| TaqI | TCGA | 7 cut(s) | 379, 486, 1864, 2056, 2440, 2825, 3073 |
| TatI | WGTACW | 1 cut(s) | 2319 |
| TauI | GCSGC | 3 cut(s) | 91, 98, 123 |
| TfiI | GAWTC | 7 cut(s) | 141, 1342, 1861, 1921, 2328, 2962, 3149 |
| TscAI | CASTG | 6 cut(s) | 362, 1135, 1684, 1731, 2941, 2956 |
| TseFI | GTSAC | 7 cut(s) | 319, 367, 397, 1166, 2720, 2951, 3018 |
| TseI | GCWGC | 6 cut(s) | 9, 111, 182, 1392, 2390, 2837 |
| Tsp45I | GTSAC | 7 cut(s) | 319, 367, 397, 1166, 2720, 2951, 3018 |
| TspGWI | ACGGA | 1 cut(s) | 278 |
| TspRI | CASTG | 6 cut(s) | 362, 1135, 1684, 1731, 2941, 2956 |
| Tth111I | GACNNNGTC | 1 cut(s) | 29 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 2561, 2849 |
| Vha464I | CTTAAG | 1 cut(s) | 469 |
| VneI | GTGCAC | 1 cut(s) | 1722 |
| VpaK11BI | GGWCC | 1 cut(s) | 1826 |
| XapI | RAATTY | 6 cut(s) | 654, 930, 1486, 1714, 2443, 2592 |
| XbaI | TCTAGA | 1 cut(s) | 1528 |
| XceI | RCATGY | 5 cut(s) | 2377, 2469, 2738, 2947, 3141 |
| XhoI | CTCGAG | 1 cut(s) | 485 |
| XmaJI | CCTAGG | 1 cut(s) | 1552 |
| XmiI | GTMKAC | 2 cut(s) | 379, 2886 |
| XmnI | GAANNNNTTC | 3 cut(s) | 342, 1581, 2332 |